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FIGURE 5. B, Induction of β-defensin 2 in human epithelial cells triggered by meso-DAP. Oral epithelial HO-1-u-1, pharyngeal epithelial HEp-2, esophageal epithelial TE-1, colonic epithelial SW620, and cervical epithelial HeLa cells were incubated for 24 h in the presence or absence of 100 µg/ml meso-DAP, DD-DAP, or LL-DAP. After fixation, cells were treated with anti-β-defensin 2 Ab or goat IgG and then visualized with Alexa Fluor 488 (green). Nuclei were visualized by staining with DAPI blue. Scale bar, 20 µM.
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