Cytoplasmic Linker Protein-170 Enhances Spreading and Phagocytosis in Activated Macrophages by Stabilizing Microtubules
J Immunol Binker et al.
179: 3780
Data Supplement
Files in this Data Supplement:
Video 1 (MOV, 4.48 Mb)
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CLIP-170 exhibits the typical MT plus-end tracking behavior in control resting macrophages. Live cell time-lapse imaging of a resting RAW 264.7 cell transfected with WT-CLIP-170-GFP to visualize CLIP-170 dynamics. Fluorescent images were acquired every 3 sec for 180 sec.
Video 2 (MOV, 6.06 Mb)
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CLIP-170 is present in long tracks in activated macrophages. Live cell time-lapse imaging of an IFN-g/LPS activated RAW 264.7 cell transfected with WT-CLIP-170-GFP to visualize CLIP-170 dynamics. Fluorescent images were acquired every 3 sec for 180 sec.
Video 3 (MOV, 2.83 Mb)
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CLIP-170 exhibits the typical MT plus-end tracking behavior in control (vehicle) resting macrophages. Live cell time-lapse imaging of a control resting RAW 264.7 cell transfected with WT-CLIP-170-GFP to visualize CLIP-170 dynamics. Fluorescent images were acquired every 3 sec for 180 sec.
Video 4 (MOV, 3.82 Mb)
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Resting macrophages treated with OA show CLIP-170 along long tracks. Live cell time-lapse imaging of an OA-treated resting RAW 264.7 cell transfected with WT-CLIP-170-GFP to visualize CLIP-170 dynamics. Fluorescent images were acquired every 3 sec for 180 sec.