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The duration of nuclear residence of NFAT determines the pattern of cytokine expression in human SCID T cells

Feske, S., R. Draeger, H.-H. Peter, K. Eichmann, and A. Rao. 2000. The duration of nuclear residence of NFAT determines the pattern of cytokine expression in human SCID T cells. J. Immunol. 165: 297–305 .

Stefan Feske and Anjana Rao wish to correct errors made in the preparation of Figs. 2, 3, 4, and 5. The co-authors (Ruth Draeger, Hans-Hartmut Peter, and Klaus Eichmann), who were not involved in the preparation of the manuscript, bear no responsibility for these mistakes. The integrity of the data and the conclusions of the paper are not affected.

In Fig. 2, no indication was given that bands with "smiles" were straightened and that lanes for the 15-min treatment with cyclosporin A were repositioned from the center to the right side in seven of the nine gel photographs. Two photographs in the upper row (left and right) and two in the middle row (left and middle) were later duplicated as Fig. 1a, A and C in the following article: Feske, S., J. Giltnane, R. Dolmetsch, L. M. Staudt, and A. Rao. 2001. Gene regulation mediated by calcium signals in T lymphocytes. Nat. Immunol. 2: 316–324. For Figs. 2 and 3, please see the corrigendum for the Nature Immunology article acknowledging initial publication of the figures in The Journal of Immunology.

In Fig. 3, photographs d and e represent 15-min, not 30-min time points, and photograph m represents 30 min, not 60 min. Controls for this experiment were from more than one source; therefore, the label should read "Co" not "Co1." Photographs in Fig. 3, panels a, b, d, and e originally published in The Journal of Immunology were later duplicated as Fig. 1b, panels A, B, C, and D in the Nature Immunology article referenced in the previous paragraph.

In Fig. 4, photograph "l" (L) was from patient 2 (P2), not patient 1 (P1), and does not represent the indicated treatment. The label for the controls should be "Co" because the experiment used sources in addition to "Co1" and "Co2." The corrected Fig. 4 with panel "l" showing the correct patient and the treatment as described in the figure legend and the corrected figure legend is shown below.

View larger version (85K): [in this window] [in a new window]   FIGURE 4. Low level translocation of NFAT1 in SCID T cell lines in the presence of the nuclear export inhibitor LMB. Control (Co) T cell lines and T cell lines from the two SCID patients (P1 and P2) were left unstimulated (a–d) or were stimulated with 1 µM ionomycin for 30 min (e–m) in the presence of 0.8 mM extracellular calcium. LMB (200 nM) was added 30 min before stimulation (i–m). Cells were spun onto poly-L-lysine-coated coverslips, fixed, and stained for NFAT1. Diagrams represent the average percentage of cells with cytoplasmic, cytoplasmic plus nuclear, or nuclear immunofluorescence, corresponding to i–m. One representative experiment of three is shown.

In Results, under the heading No inherent defect of NFAT or CN in the SCID patients’ T cells, the reference to "60 min" in the last sentence of the first paragraph is incorrect. The corrected sentence should read: "Cytoplasmic extracts from patient and control lines were incubated with CN plus calmodulin for 20 and 70 min at 30°C, and NFAT1 was detected by Western blotting (Fig. 5A), revealing the same amount and kinetics of dephosphorylation in control and patient T cells."

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