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The Journal of Immunology, 2007, 179, 7891-7898
Copyright © 2007 by The American Association of Immunologists, Inc.
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Tissue- and Stimulus-Dependent Role of Phosphatidylinositol 3-Kinase Isoforms for Neutrophil Recruitment Induced by Chemoattractants In Vivo1

Vanessa Pinho2,{dagger}, Remo de Castro Russo*, Flávio A. Amaral*, Lirlândia P. de Sousa*, Michele M. Barsante*, Danielle G. de Souza§, José C. Alves-Filho, Denise C. Cara{ddagger}, Joel S. Hayflick||, Christian Rommel#, Thomas Ruckle#, Adriano G. Rossi** and Mauro M. Teixeira*

* Departamento de Bioquímica e Imunologia, {dagger} Departamento de Morfologia, {ddagger} Departamento de Patologia, and § Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil; Departmento de Farmacologia, Escola de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil; || ICOS Corporation, Bothell, WA 98021; # Merck Serono International, Geneva, Switzerland; and ** Medical Research Council Centre for Inflammation Research, Queen’s Medical Research Institute, University of Edinburgh, Edinburgh, United Kingdom


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
PI3K plays a fundamental role in regulating neutrophil recruitment into sites of inflammation but the role of the different isoforms of PI3K remains unclear. In this study, we evaluated the role of PI3K{gamma} and PI3K{delta} for neutrophil influx induced by the exogenous administration or the endogenous generation of the chemokine CXCL1. Administration of CXCL1 in PI3K{gamma}–/– or wild-type (WT) mice induced similar increases in leukocyte rolling, adhesion, and emigration in the cremaster muscle when examined by intravital microscopy. The induction of neutrophil recruitment into the pleural cavity or the tibia-femoral joint induced by the injection of CXCL1 was not significantly different in PI3K{gamma}–/– or WT mice. Neutrophil influx was not altered by treatment of WT mice with a specific PI3K{delta} inhibitor, IC87114, or a specific PI3K{gamma} inhibitor, AS605240. The administration of IC87114 prevented CXCL1-induced neutrophil recruitment only in presence of the PI3K{gamma} inhibitor or in PI3K{gamma}–/– mice. Ag challenge of immunized mice induced CXCR2-dependent neutrophil recruitment that was inhibited by wortmannin or by blockade of and PI3K{delta} in PI3K{gamma}–/– mice. Neutrophil recruitment to bronchoalveolar lavage induced by exogenously added or endogenous production of CXCL1 was prevented in PI3K{gamma}–/– mice. The accumulation of the neutrophils in lung tissues was significantly inhibited only in PI3K{gamma}–/– mice treated with IC87114. Neutrophil recruitment induced by exogenous administration of C5a or fMLP appeared to rely solely on PI3K{gamma}. Altogether, our data demonstrate that there is a tissue- and stimulus-dependent role of PI3K{gamma} and PI3K{delta} for neutrophil recruitment induced by different chemoattractants in vivo.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Phosphatidylinositol 3-kinases are a family of proteins that catalyze the phosphorylation of the 3'-OH position of the inositol ring of phosphatidylinositols (PtdIns)3 resulting in the formation of PtdIns(3)P, PtdIns(3,4)P2, and PtdIns(3,4,5)P3, collectively termed 3'-PtdIns lipids (1, 2). These molecules work as second messengers and are important in regulation of various cellular responses, including proliferation, transformation, survival, superoxide production, adhesion, and cell migration (3). There are different isoforms of PI3Ks that can be divided into three main classes. The class I PI3Ks are heterodimers consisting of a catalytic subunit and an adaptor/regulatory subunit present mainly in the cytosol of cells and can be subdivided into two subclasses: class IA enzymes may have one of three catalytic isoforms (p110{alpha}, p110β, or p110{delta}) associated to five regulatory isoforms and signal downstream of tyrosine kinases; class IB enzymes are constituted by a unique catalytic subunit (p110{gamma}) associated with a regulatory subunit p101 and signal downstream of heterotrimeric G protein-coupled receptors (GPCRs) (1, 2). There is also a class II PI3K, characterized by the presence of a C2 domain, and a class III PI3K, which uses only PtdIns as a substrate (1, 2).

Neutrophils, a major type of blood leukocytes, are indispensable for host defense of bacterial and fungal infections (4). This activity depends on the stimulation of various functions after activation of specific receptors, including the formyl methionyl leucyl peptide, chemotactic factor, and TLRs (5, 6, 7, 8, 9, 10). Neutrophil migration is dependent on their activation by chemotactic stimuli, including chemokines, complement fragments, and lipid mediators, which enable these cells to enter the site of infection and destroy the invading pathogens. Chemotactic factors bind to seven-transmembrane domain receptors and activate heterotrimeric G proteins (5, 6, 11, 12). Downstream of these proteins a complex interrelated signaling network is activated and may result in the activation of PI3K isoforms (12, 13, 14, 15). Involvement of PI3Ks in neutrophil activation has been demonstrated using the inhibitors of class I PI3K, wortmannin and LY294002, which block neutrophil activation and PtdIns(3,4,5)P3 generation, a main product of PI3K activation (16, 17). Studies using mice lacking the p110 catalytic subunit of PI3K{gamma} have demonstrated the importance of this PI3K isoform for chemokine-regulated recruitment of neutrophils in vitro (18, 19). Furthermore, PI3K{gamma}-deficient neutrophils exhibited severe defects in GPCR-induced respiratory burst and produced less PtdIns(3,4,5)P3 after stimulation of cells with C5a, fMLP, and CXCL8 (IL-8) (19). It has been recently suggested that PI3K{delta} may also modulate the activity and recruitment of neutrophils in vivo (20, 21). However, the exact extent to which PI3K{gamma} participates in the process of neutrophil recruitment in vivo is not known.

The present study was conducted initially to investigate the role of PI3K{gamma} in modulating neutrophil rolling, adhesion, and transmigration induced by the chemokine CXCL1 in the mouse cremaster. However, as PI3K{gamma} appeared not to be directly involved with CXCL1-induced neutrophil influx, further studies were conducted to investigate whether the enzyme was relevant for the neutrophil recruitment in other sites of inflammation and that induced by more complex inflammatory stimuli, including Ag challenge or bleomycin administration. We also evaluated the relevance of PI3K{gamma} for neutrophil influx induced by other chemoattractant molecules and whether PI3K{delta} played a role in CXCL1-induced neutrophil influx. We demonstrate that the involvement of PI3K{gamma} on neutrophil recruitment depends on the chemotactic stimulus and on the tissue in which the inflammatory stimulation is occurring. PI3K{delta} and PI3K{gamma} have a redundant role in mediating exogenously added or endogenously generated CXCL1-induced neutrophil recruitment.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 Disclosures
 References
 
Animals

PI3K{gamma}-deficient male C57BL/6 x sv129 mice and their wild-type (WT) littermate control mice (+/+) (18–22 g) were used throughout these experiments. PI3K{gamma}–/– mice (in a C57BL/6 x sv129 background) were generated as previously described (18). The mice were a gift of Dr. J. M. Penninger (Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria) and supplied by Taconic Farms. PI3K{gamma} gene deletion was confirmed by PCR using specific primers (WT primers: sense, 5-TCAGGCTCGGAGATTAGGTA-3; antisense, 5-GCCCAATCGGTGGTAGAACT-3; PI3K{gamma}–/– primers: sense, 5-GGACACGGCTTTGATTACAATC-3; antisense primer, 5-GGGGTGGGATTAGATAAATG-3 as previously described (18). Animals were bred and housed in a temperature-controlled room with free access to water and food. All experimental protocols have been subjected to evaluation and were approved by the local animal ethics committee.

Drugs and reagents

Recombinant murine CXCL1 was purchased from PeproTech. CXCL1 was dissolved in water, diluted further in PBS (pH 7.4) containing 0.01% BSA and stored at –70°C until use. OVA was purchased from Sigma-Aldrich and C5a and wortmannin were purchased from Calbiochem. Wortmannin was diluted in DMSO and stored at –70°C until use. LY294002 was purchased from Alamone Laboratories, diluted in DMSO, and stored at –70°C until use. Bleomycin (blenoxane; Bristol-Meyers) was commercially available. The PI3K{delta} inhibitor IC87114 was a gift of the ICOS Corporation and stored at –20°C until use. The PI3K{gamma} inhibitor AS605240 was a gift of Serono and was stored at –20°C until use.

Intravital microscopy

The mouse cremaster preparation was used to study the behavior of leukocytes in the microcirculation and adjacent connective tissue, as previously described (22). Briefly, an incision was made in the scrotal skin to expose the left cremaster muscle, which was then carefully removed from the associated fascia. A lengthwise incision was made on the ventral surface of the cremaster muscle using a cautery. The testicle and the epididymis were separated from the underlying muscle and were moved into the abdominal cavity. The muscle was then spread out over an optically clear viewing pedestal and was secured along the edges with a 4-0 suture. The exposed tissue was superfused with warm bicarbonate-buffered saline (pH 7.4). An intravital microscope (Olympus BX50F4) with a x20 objective lens and a x10 eyepiece was used to examine the cremasteric microcirculation. A video camera (5100 HS; Panasonic) was used to project the images onto a monitor, and the images were recorded for playback analysis using a conventional videocassette recorder.

Single, unbranched cremasteric venules (25–40 µm in diameter) were selected and, to minimize variability, the same section of cremasteric venule was observed throughout the experiment. The number of rolling, adherent, and emigrated leukocytes was determined offline during video playback analysis. Rolling leukocytes were defined as those cells moving at a velocity less than that of erythrocytes within a given vessel. The flux of rolling cells was measured as the number of rolling cells passing by a given point in the venule per minute. A leukocyte was considered to be adherent if it remained stationary for at least 30 s, and total leukocyte adhesion was quantified as the number of adherent cells within a 100-µm length of venule. Leukocyte emigration was defined as the number of cells in the extravascular space within an area of 50 µm of distance from the venule. Only cells adjacent to and clearly outside the vessel under study were counted as emigrated.

Experimental protocols

For each experiment, 500 ng of murine rCXCL1 in 0.2 ml of saline was administered locally by s.c. injection beneath the right scrotal skin using a 30-G needle, 2 h before exteriorization. The left cremaster was then prepared for intravital microscopy. Leukocyte kinetics was investigated, as described above, and at the end of each experiment whole blood was drawn by cardiac puncture. Total cell counts were performed in a modified Neubauer chamber using Turk’s stain.

Histology

At the end of each intravital microscopy experiment, the cremaster muscles were removed and fixed in 10% neutral-buffered formalin. The tissues were dehydrated gradually in ethanol, embedded in paraffin, cut into 4-µm sections, stained with H&E, and examined under direct light microscopy.

Sensitization

Animals were immunized with OVA adsorbed to aluminum hydroxide gel as previously described (23). Briefly, mice were injected s.c. on days 1 and 8 with 0.2 ml of a solution containing 100 µg of OVA and 70 µg of aluminum hydroxide (Reheiss).

Leukocyte migration into the pleural cavity induced by Ag and chemoattractant agent

Sensitized WT or PI3K{gamma}–/– mice were challenged by intrapleural (i.pl.) administration of Ag (OVA) or PBS. In addition, to investigate the leukocyte migration into the pleural cavity induced by a chemoattractant agent, WT or PI3K{gamma}–/– mice were injected into the pleural cavity with CXCL1 (30–100 ng/cavity), C5a (100 ng/cavity), and fMLP (200 ng/cavity). These were optimal doses to induce leukocyte recruitment in preliminary experiments (data not shown). The cells present in the pleural cavity were harvested 6 h after Ag challenge or chemoattractant agent administration by injecting 2 ml of PBS and total cell counts performed in a modified Neubauer chamber using Turk’s stain. This time was shown to be optimal for neutrophil recruitment in preliminary kinetics experiments. Differential cell counts were performed on cytospin preparations (Shandon III) stained with May-Grünwald-Giemsa using standard morphologic criteria to identify cell types. The results are presented as the number of cells per cavity.

Leukocyte migration into the lung induced by chemoattractant agent or bleomycin

WT or PI3K{gamma}–/– mice were injected intratracheally with 500 ng of murine rCXCL1 or 0.125 U of bleomycin. The stimulus was given in 50 µl of sterile PBS. The control mice received PBS alone. The cells present in the alveolar space and the lung were harvested 6 h after CXCL1 administration or 48 h after bleomycin administration.

Bronchoalveolar lavage (BAL)

BAL was performed to obtain leukocytes in the alveolar spaces. The trachea was exposed and a 1.7-mm outside-diameter polyethylene catheter was inserted. BAL was performed by instilling three 1-ml aliquots of PBS and ~2 ml of fluid was retrieved per mouse. The number of total leukocytes was determined by counting leukocytes in a modified Neubauer chamber after staining with Turk’s solution. Differential counts were obtained from cytospin preparations by evaluating the percentage of each leukocyte on a slide stained with May-Grünwald-Giemsa.

Quantification of neutrophil accumulation in lung tissue by myeloperoxidase (MPO) activity measurement

The extent of neutrophil accumulation in the lung tissue was measured by assaying MPO activity as previously described (24). Using the conditions described below, this methodology is very selective for the determination of neutrophils over macrophages (data not shown). Before lung removal, the pulmonary vasculature was perfused with 3 ml of PBS via the right ventricle. Upon thawing, the tissue (0.1 g of tissue per 1.9 ml of buffer) was homogenized in pH 4.7 buffer (0.1 M NaCl, 0.02 M NaPO4, 0.015 M sodium EDTA), centrifuged at 3000 x g for 10 min and the pellet was subjected to hypotonic lyses (1.5 ml of 0.2% NaCl solution followed 30 s later by addition of an equal volume of a solution containing 1.6% NaCl and 5% glucose). After a further centrifugation, the pellet was resuspended in 0.05 M NaPO4 buffer (pH 5.4) containing 0.5% hexadecyl-trimethylammonium bromide and rehomogenized. One-milliliter aliquots of the suspension were transferred into 1.5-ml Eppendorf tubes followed by three freeze-thaw cycles using liquid nitrogen. The aliquots were then centrifuged for 15 min at 3000 x g to perform the assay. MPO activity in the resuspended pellet was assayed by measuring the change in OD450 nm using tetramethylbenzidine (1.6 mM) and H2O2 (0.5 mM). The reaction was stopped by adding 100 µl of 4 M H2SO4 and was quantified at 450 nm in a spectrophotometer (Emax; Molecular Devices). The neutrophil content was calculated from a standard curve based on MPO activity expressed as absorbance increase at 450 nm from 5% casein peritoneal-induced neutrophils assayed in parallel. The results were expressed in relative number of neutrophils per milligram of wet tissue.

Leukocyte migration into an articular cavity

The role of PI3K{gamma} for neutrophil recruitment was also investigated after administration of CXCL1 in articular cavity. WT or PI3K{gamma}–/– mice were injected into the tibia-femoral articulation with an optimal dose of murine rCXCL1 (30 ng/cavity). The stimulus was given in 5 µl of sterile PBS and control mice received PBS alone. The cells present in the tibia-femoral articulation were harvested 6 h after chemoattractant agent administration by washing the cavity with 30 µl of PBS. The number of total leukocytes was determined by counting leukocytes in a modified Neubauer chamber after staining with Turk’s solution. Differential counts were obtained from cytospin preparations by evaluating the percentage of each leukocyte on a slide stained with May-Grünwald-Giemsa.

Treatment with inhibitors of PI3K

The role of PI3K on neutrophils recruitment was also investigated by using selective inhibitors of PI3K, wortmannin and LY294002; the selective inhibitor of PI3K{delta}, IC87114; and selective inhibitor of PI3K{gamma}, AS605240. Wortmannin or LY294002 were administered systemically (i.p.) at the dose of 1.0 mg/kg 60 min before the i.pl. administration of OVA or chemoattractant agent. This dose was shown to be effective in other experimental systems (25). Moreover, preliminary experiments showed the dose of 1.0 mg/kg to be maximally effective at inhibiting eosinophil recruitment in a similar model (26). IC87114, a highly selective inhibitor of PI3K{delta} (20), was administered orally at the dose of 25 mg/kg 60 min before administration of chemoattractant agents. This dose was shown to be effective and selective in another model of leukocyte influx (21). AS605240, a highly selective inhibitor of PI3K{gamma} (27), was administered orally at the dose of 50 mg/kg 60 min before administration of chemoattractant agents. This dose was shown to be effective and selective in vivo (27). Wortmannin and LY294002 were dissolved in DMSO and further diluted in PBS. IC87114 and AS605240 was suspended in 0.1% methylcellulose solution and ground in a homogenizer to ensure a uniform suspension. Control animals received drug vehicle.

Preparation of bone marrow neutrophils

The femurs and tibias from both hind limbs were removed of mice and freed of soft tissue attachments, and the extreme distal tip of each extremity was cut off. HBSS (without Ca2+ and Mg2+) was forced through the bone by using a 5-ml syringe with a 22-gauge needle. After dispersing cell clumps and removing the debris, the bone marrow cells were suspended in 2 ml of HBSS and laid on top of a two-layer Percoll gradient of 72 and 65% Percoll (Sigma-Aldrich) diluted in HBSS (100% Percoll = nine parts Percoll and one part 10x HBSS) and centrifuged (1200 x g, 30 min, room temperature) without braking. The enriched neutrophil fraction was recovered at the interface between 65 and 72% Percoll. After washing twice with HBSS, 5.0 ± 1.0 x 106 cells were obtained per mouse, 95% neutrophils, identified by staining the nuclei with Turk reagent (Merck).

Neutrophil chemotaxis

A modified Boyden chamber assay to examine the neutrophil chemoattractant response to CXCL-1 and C5a was performed using a 48-well microchamber (Neuro Probe). Murine bone marrow neutrophils were isolated as above described and resuspended in running buffer (1x HBSS, 2 mg/ml BSA, 10 mM HEPES, 1 mM CaCl2, and 1 mM MgCl2). C5a (10 nM) and recombinant mouse CXCL-1 (20 ng/ml) diluted in running buffer (for wells containing neutrophils) or appropriate buffer control was added to the lower chambers of the apparatus. A 5-µm-pore polycarbonate membrane (Neuro Probe) was placed between the upper and lower chambers, and 5 x 104 cells in a volume of 50 µl were added to the top chambers of the apparatus. Cells were allowed to migrate into the membrane for 1 h per treatment at 37°C with 5% CO2. Following incubation, the chamber was disassembled and the membrane was scraped and washed three times in PBS to remove nonadherent cells before being fixed in methanol and stained using the Diff-Quik system (Dade Behring). Each well-associated membrane area was scored using light microscopy to count the intact cells present in five random fields. The results are expressed as the number of neutrophils per field.

Statistical analysis

All results are presented as the mean ± SEM. Normalized data were analyzed by one-way ANOVA, and differences between groups were assessed using the Student-Newman-Keuls posttest. A p value <0.05 was considered significant.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 Disclosures
 References
 
Neutrophil recruitment induced by the i.pl. administration of 100 ng of CXCL1 was similar in PI3K{gamma}–/– and WT mice (Fig. 1A). Neutrophil recruitment induced by lower doses (30 ng/cavity) of CXCL1 was also similar in PI3K{gamma}–/– and WT mice (WT mice: 3.1 ± 0.6 x 105 neutrophils/cavity; PI3K{gamma}–/– mice: 2.1 ± 0.4 x 105 neutrophils/cavity). Similar results were obtained using intravital microscopy in the CXCL1-treated cremaster muscle of PI3K{gamma}–/– or WT mice. The dose of CXCL1 used in these experiments was based in preliminary experiments and previous studies (28). Intrascrotal administration of 500 ng of CXCL1 induced similar increases in leukocyte rolling (WT mice: 50.6 ± 4.3 cell/min; PI3K{gamma}–/– mice: 75.6 ± 8.0 cell/min), adhesion, and emigration in PI3K{gamma}–/– or WT mice (Fig. 2). To identify the type of cells that had been recruited to the site of inflammation at this time point, H&E staining was performed on the tissues after the experiment. Neutrophils were the majority of the cells attached to endothelial cells or recruited to the cremaster muscle sections of both PI3K{gamma}–/– or WT mice (data not shown). The circulating leukocyte counts were not different in these mice after administration of PBS or CXCL1 (Table I).


Figure 1
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  		FIGURE 1. Effects of the pretreatment with the nonselective PI3K inhibitor (A), wortmannin, and PI3K{delta}-selective inhibitor (B), IC87114, and PI3K{gamma}-selective inhibitor, AS605240, on the recruitment of neutrophils induced by CXCL1 in WT and PI3K{gamma}–/– mice. Wortmannin (1.0 mg/kg), IC87114 (25 mg/kg), and AS605240 (50 mg/kg) was administered 60 min before CXCL1 (100 ng/cavity) i.pl. administration. Neutrophil recruitment was assessed 6 h after chemokine injection. Results are expressed as the means ± SEM of five mice in each group. *, p < 0.01 when compared with PBS-injected mice; #, p < 0.01 when compared with vehicle-treated CXCL1-injected mice.

 

Figure 2
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  		FIGURE 2. Neutrophil adhesion (A) and emigration (B) in the cremaster muscle of WT and PI3K{gamma}–/– mice 2 h after intrascrotal administration of CXCL1 (500 ng/animal). Control mice were injected with PBS. Results are expressed as the means ± SEM of four to five mice in each group. *, p < 0.01 when compared with PBS-injected mice.

 

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  		Table I. Circulating leukocyte counts and venular diameter in untreated mice and mice treated intrascrotally with CXCL1 (500 ng)a

 
The next series of experiments were conducted in the pleural cavity for ease of performance. Despite the similar neutrophil recruitment in PI3K{gamma}–/– and WT mice, treatment with the isoform nonselective PI3K inhibitor wortmannin (1.0 mg/kg) inhibited CXCL1-induced neutrophil recruitment both in PI3K{gamma}–/– and WT mice (Fig. 1A). The latter results suggested that a PI3K was indeed relevant for chemokine-induced neutrophil influx. Treatment of WT mice with IC87114, a PI3K{delta} inhibitor, or AS605240, a PI3K{gamma} inhibitor, alone failed to affect the neutrophil recruitment induced by CXCL1 (Fig. 1B). However, the treatment of PI3K{gamma}–/– mice with IC87114 and the concomitant treatment with both IC87114 and AS605240 significantly inhibited CXCL1-induced neutrophil recruitment (Fig. 1B). In vitro, the chemotaxis of bone marrow-derived neutrophils obtained from PI3K{gamma}–/– mice was decreased in comparison to their WT control (basal chemotaxis: WT, 5.0 ± 0.4 neutrophils/field; PI3K{gamma}–/–, 4.3 ± 0.5 neutrophils/field; CXCL1-induced chemotaxis: WT, 18.5 ± 0.2 neutrophils/field; PI3K{gamma}–/–, 10.5 ± 0.4 neutrophils/field, p < 0.05).

We then examined whether PI3K{gamma} deficiency might alter the neutrophil recruitment induced by endogenously generated CXCL1, i.e., after Ag challenge (OVA, 1 µg/cavity) of sensitized mice. The i.pl. injection of OVA in sensitized WT mice induced a maximal neutrophil recruitment that occurred between 6 and 12 h and had dropped to baseline levels 24 h later (Fig. 3A). The neutrophil accumulation after Ag challenge was not altered in PI3K{gamma}-deficient mice (Fig. 3A). Treatment of sensitized WT mice with a CXCR2 antagonist greatly reduced OVA-induced neutrophil accumulation (PBS, 1.8 ± 0.7 x 105 neutrophil/cavity; OVA, 10.0 ± 2.6 x 105 neutrophils/cavity; OVA + CXCR2 antagonist, 2.9 ± 1.6 x 105 neutrophils/cavity, n = 5, p < 0.05), demonstrating that the neutrophil recruitment induced by Ag challenge of sensitized mice depended mainly on CXC chemokine production and CXCR2 activation. Treatment with wortmannin or LY294002, two structurally distinct PI3K inhibitors, diminished the accumulation of neutrophils induced by Ag challenge (Fig. 3B). Treatment of WT mice with IC87114 did not affect the neutrophil recruitment induced by Ag challenge of sensitized mice. However, the treatment of PI3K{gamma}–/– mice with IC87114 diminished the influx of neutrophils into the pleural cavity (Fig. 3C).


Figure 3
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  		FIGURE 3. Role of PI3K in neutrophil accumulation induced by Ag challenge of sensitized PI3K{gamma}–/– and WT mice. Neutrophil accumulation was assessed after 6 h in immunized WT or PI3K{gamma}–/– mice and challenged with an i.pl. injection of OVA (1 µg/cavity) or PBS at the time point described in the figure (A). Effects of the pretreatment with the nonselective PI3K inhibitors (B), wortmannin (1.0 mg/kg), and LY294002 (1.0 mg/kg), and PI3K{delta}-selective inhibitor (C), IC87114 (25 mg/kg), in the neutrophil accumulation induced by Ag were also verified. Results are expressed as the means ± SEM of five mice in each group. *, p < 0.05 when compared with PBS-injected mice; #, p < 0.05 when compared with vehicle-treated OVA-injected WT mice.

 
Using an in vitro chemotaxis assay, it has been previously shown that C5a-induced chemotaxis is impaired in neutrophils from PI3K{gamma}–/– mice (19). In experiments with bone marrow-derived neutrophils, C5a-induced chemotaxis was partially diminished in PI3K{gamma}–/– mice vs WT (basal chemotaxis: WT, 5.0 ± 0.4 neutrophils/field; PI3K{gamma}–/–, 4.3 ± 0.5 neutrophils/field; C5a-induced chemotaxis: WT, 17.8 ± 1.2 neutrophils/field; PI3K{gamma}–/–, 11.4 ± 1.2 neutrophils/field, p < 0.05). In our in vivo experiments, the recruitment of neutrophils induced by i.pl. administration of C5a was abrogated in PI3K{gamma}–/– mice (Fig. 4A). Similarly, neutrophil recruitment induced by the administration of the chemoattractant molecule fMLP was abrogated in PI3K{gamma}–/– mice as compared with their WT controls (Fig. 4B).


Figure 4
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  		FIGURE 4. Role of PI3K in neutrophil accumulation induced by C5a and fMLP in PI3K{gamma}–/– and WT mice. A, C5a (100 ng/cavity) or, B, fMLP (200 ng/cavity) were injected in pleural cavity and neutrophil accumulation was assessed 6 h later. Results are expressed as the means ± SEM of five mice in each group. *, p < 0.01 when compared with PBS-injected mice; #, p < 0.01 when compared with OVA-injected WT mice.

 
The recruitment of neutrophils to lung tissue and BAL fluid was evaluated after instillation of CXCL1 or bleomycin. Neutrophil influx to alveolar spaces was totally prevented in PI3K{gamma}-deficient mice (Fig. 5A). In contrast, the deficiency of PI3K{gamma} had no effect on CXCL1-induced recruitment of neutrophils to lung tissues of mice, as assessed by measuring tissue MPO content (Fig. 5B). Treatment of WT mice with IC87114 failed to affect CXCL1-induced recruitment of neutrophils to lung tissues (Fig. 5C). However, the amount of neutrophils in lung tissues was significantly inhibited in PI3K{gamma}–/– mice treated with IC87114 (Fig. 5C).


Figure 5
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  		FIGURE 5. Role of PI3K in neutrophil recruitment to lung after intratracheal administration of CXCL1. CXCL1 (500 ng/animal) was administered intratracheally in WT and PI3K{gamma}–/– mice. The BAL and lung removal was performed 6 h after chemokine administration. A, Neutrophil influx to alveolar spaces; B, CXCL1-induced recruitment of neutrophil to lung tissues assessed by measuring tissue MPO content; and C, CXCL1-induced recruitment of neutrophil to lung tissues assessed by measuring tissue MPO content after IC87114 treatment. Results are expressed as the means ± SEM of five mice in each group. *, p < 0.01 when compared with PBS-injected mice; #, p < 0.01 when compared with CXCL1-injected WT mice.

 
Bleomycin-induced neutrophil influx depends of the release of CXCL1 into the alveolar space and activation of CXCR2 receptors (R. de Castro Russo and M. M. Teixeira, unpublished data). In agreement with the findings after CXCL1 administration, neutrophil influx to alveolar space after bleomycin administration was markedly diminished in PI3K{gamma}–/– as compared with WT mice (Fig. 6A) and the recruitment of neutrophils to lung tissues was unaltered in PI3K{gamma}–/– mice given bleomycin (Fig. 6B).


Figure 6
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  		FIGURE 6. Role of PI3K in neutrophil recruitment to lung after intratracheal administration of bleomycin. Bleomycin (0.125 U/animal) was administered intratracheally in WT and PI3K{gamma}–/– mice. Lung removal was performed 48 h after bleomycin administration. A, Neutrophil influx to alveolar spaces and, B, bleomycin-induced recruitment of neutrophil to lung tissues assessed by measuring tissue MPO content. Results are expressed as the means ± SEM of five mice in each group. *, p < 0.01 when compared with PBS-injected mice; #, p < 0.01 when compared with OVA-injected WT mice.

 
Furthermore, the role of PI3K{gamma} in neutrophil recruitment induced by CXCL1 was further confirmed in another model of neutrophil recruitment using the tibia-femoral joint. Neutrophil influx into the tibia-femoral joint was not significantly affected in PI3K{gamma}-deficient mice (number of neutrophils x 106/cavity: WT mice: PBS, 1.2 ± 0.9; CXCL1, 12.8 ± 5.9; PI3K{gamma}–/– mice: PBS, 0.2 ± 0.1; CXCL1, 12.4 ± 6.4, n = 5, p > 0.05 when comparing WT vs PI3K{gamma}–/– mice).


   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
Disclosures
 References
 
The present study investigated the role of PI3K{gamma} and PI3K{delta} for neutrophil influx induced by the exogenous administration or the endogenous generation of the chemokine CXCL1. Overall, administration of CXCL1 induced leukocyte migration that was similar in PI3K{gamma}–/– or WT mice, or in mice treated with a specific PI3K{delta} inhibitor, IC87114, or a specific PI3K{gamma} inhibitor, AS605240. CXCR2-dependent neutrophil recruitment was only inhibited when there was concomitant inhibition of both PI3K{delta} and PI3K{gamma}. In contrast, neutrophil recruitment into alveolar space or in response to exogenous administration of C5a or fMLP appeared to rely solely on PI3K{gamma}.

We demonstrated that PI3K{gamma} deficiency did not interfere significantly with CXCL1-induced rolling, adhesion, and transmigration of neutrophil in cremaster muscle. Recently, it was shown that PI3K{gamma}-deficient mice presented a decrease in CXCL1-induced leukocyte adhesion in venules of the exteriorized mouse cremaster muscle (29). However, the experimental conditions used by the authors differ from ours in an important point. Although the chemotactic stimulus was injected i.v. and leukocyte adhesion was counted 1 min after the administration of CXCL1, CXCL1 was given locally (i.e., into the tissue) in our experiments and rolling, adhesion, and emigration were evaluated 2 h later. Thus, whereas in our experiments the inflammatory stimulus was present in the tissues where they are usually generated, in the previous study (29), the chemokine was given i.v. In the latter case, there may be stimulation (activation) of leukocytes before they migrate into tissue permitting leukocyte adherence in vessels but not their migration into tissue. Leukocyte activation may mimic the situation of chemotaxis in vitro and neutrophil chemotaxis induced by chemotactic agents is dependent on PI3K{gamma} (Ref. 30 and our results). In contrast, injection of stimulus in the tissue was PI3K{gamma} independent and correlated better with the PI3K{gamma}-independent chemokinetic activity of neutrophils (30, 31). More recently, Liu et al. (38) showed that neutrophil emigration in response to CXCL1 was PI3K{gamma} dependent at least up to 90 min after the addition of stimulus and was PI3K{gamma} independent thereafter. The results in later time points are consistent with the PI3K{gamma}-independent neutrophil influx in response to Ag and CXCL1 in our systems. Further studies are certainly needed to understand in greater detail the relevance of these in vitro phenomena (chemotaxis vs chemokinesis) for neutrophil recruitment in vivo and how the whole process is modulated by PI3K{gamma}.

Although CXCL1-induced accumulation of neutrophils into the pleural cavity was similar in PI3K{gamma}-deficient and WT mice, treatment with an isoform nonselective PI3K inhibitor, wortmannin, inhibited neutrophil accumulation induced by CXCL1. Wortmannin was capable of preventing neutrophil influx both in WT and PI3K{gamma}–/– mice. Previous studies have demonstrated that activation of PI3K/Akt is required for the induction of human neutrophil migration in response to CXCL8 (a homolog of murine CXCL1) (6, 32). Thus, although CXC chemokine-induced neutrophil recruitment appears to be PI3K dependent, it appears that sole blockade (or absence) of PI3K{gamma} is not sufficient to prevent neutrophil influx induced by CXCL1. In contrast to our findings, Hirsch et al. (19) demonstrated that neutrophil influx induced by IL-8 was partially inhibited in PI3K{gamma}–/– mice. One possibility to explain the discrepancies between those and our findings could be the need for the stimulation of intermediate cells and molecules for human IL-8 to induce recruitment of neutrophils in rodents (33, 34).

Recent reports have also suggested a role for PI3K{delta} in promoting neutrophil trafficking in a mouse model of TNF-{alpha}-induced inflammation and in a model of acute pulmonary inflammation induced by LPS in rats (20, 21). Treatment with a PI3K{delta} inhibitor, IC87114, did not affect CXCL1-induced neutrophil recruitment into the pleural cavity of WT mice at a dose previously shown to be effective in vivo (21). However, the data with wortmannin still suggested that the recruitment of neutrophils induced by CXCL1 would still depend on the activation of other PI3K isoforms or of the joint action of diverse isoforms. Sadhu et al. (15) have proposed that different PI3K isoforms may be activated in response to in vitro stimulation of GPCRs leading to human neutrophil migration. In our experiments, we demonstrated that inhibition of PI3K{delta} in PI3K{gamma}–/– mice or treatment with inhibitors of both enzymes was accompanied by significant inhibition of neutrophil migration induced by exogenous administration of CXCL1. This result suggests that neutrophil recruitment following triggering of CXCR2 depends on the function of both PI3K{gamma} and PI3K{delta}. In human neutrophils, PI3K{gamma} and PI3K{delta} act together to amplify PIP3 production leading to cell polarization and chemotaxis (15). In accordance with the latter finding, PI3K{gamma}/PI3K{delta}–/– mice have a profound reduction of T cell development (35).

It has been shown that PI3K{gamma} deficiency affected the ability of the endothelium to adhere neutrophils, most likely due to a reduction in E-selectin-mediated rolling (36). However, deficiency in p110{gamma} and p110{delta} catalytic subunits of PI3K in the venular endothelium had an additive effect in the ability of this cell type to recruit neutrophils in response to TNF-{alpha} stimulation (36). More recent studies from the same group show that PI3K{gamma} and PI3K{delta} have temporally distinct roles in mediating leukocyte recruitment in vivo (31). Thus, PI3K{gamma} and PI3K{delta} mediate neutrophil influx in a kinetic- (31), tissue-, and stimulus- (this study) dependent manner.

A series of experiments were then conducted to examine the role of PI3K{gamma} and PI3K{delta} for the recruitment of neutrophils induced by administration of Ag to immunized mice. In this model, we show that neutrophil recruitment is totally dependent on the endogenous release of CXCR2-acting chemokines. As such, Ag-induced neutrophil recruitment was unchanged in PI3K{gamma}-deficient mice but was abrogated by treatment with PI3K-nonselective inhibitors. Administration of the PI3K{delta} inhibitor failed to alter neutrophil recruitment in WT mice. In contrast, the PI3K{delta} inhibitor greatly prevented neutrophil recruitment in Ag-challenged PI3K{gamma}-deficient mice. Altogether, the latter results demonstrate that endogenous CXCR2-acting chemokines, such as CXCL1, are necessary for Ag-induced neutrophil influx and work via activation of both PI3K{gamma} and PI3K{delta}.

Neutrophil infiltration into BAL fluid after intranasal administration of CXCL1 was greatly diminished in PI3K{gamma}-deficient mice, an effect consistent with other studies (37). However, neutrophil influx into pulmonary tissue was not dependent on PI3K{gamma}. It has been demonstrated that intravascular, interstitial, and intra-alveolar accumulation of neutrophils may be differentially regulated; indeed, polymorphonuclear neutrophils can enter the pulmonary interstitium without advancing to the alveolar airway space and crossing the epithelial barrier (38). Thus, the differential mechanisms used by neutrophils to cross endothelial and epithelial cells may explain the differential use of PI3K{gamma} for neutrophil influx into lung tissues and alveolar spaces. However, the latter possibility needs to be investigated in more detail.

We also demonstrated in a model of bleomycin-induced pulmonary injury that deficiency of PI3K{gamma} inhibited neutrophil influx into the alveolar space but did not interfere with neutrophil recruitment to lung tissue. In this model of pulmonary inflammation, neutrophil recruitment is associated with endogenous production and action of CXCL1 on CXCR2 receptors (R. de Castro Russo and M. M. Teixeira, unpublished data). Therefore, CXCL1-dependent neutrophil transmigration through lung endothelial cells depends on the cooperative action of both PI3K{gamma} and PI3K{delta}. In contrast, migration through epithelial cells is totally dependent on PI3K{gamma}.

There is evidence that GPCRs are able to activated class IA PI3K (39, 40). How might PI3K{delta} be activated by GPCRs? It is proposed that an initial burst of PIP3 induced by PI3K{gamma} activation could activate Rac and Src family tyrosine kinases that could then activate PI3K{delta} (15). It is not clear whether the latter hypothesis would be functional in our experiments, as one would expect complete blockade of migration once PI3K{gamma} or PI3K{delta} were blocked. Additional experiments are necessary to understand the interplay (or functional relationships) between these two isoforms in vivo.

We also evaluated the importance of PI3K{gamma} for neutrophil recruitment to the pleural cavity induced by the complement fragment C5a and the bacterial-derived chemotactic peptide fMLP. C5a is a potent chemoattractant for neutrophils, basophils, eosinophils, monocytes, and macrophages and is released after complement activation at sites of inflammation (41, 42). The role of PI3K{gamma} to C5a-induced neutrophil migration is supported by previous studies demonstrating the inhibition of PtdIns(3,4,5)P3 production and chemotaxis after in vitro stimulation of neutrophil with C5a in PI3K{gamma}–/– neutrophils (19). It is well-known also that neutrophil recruitment in response to Gram-negative infection involves bacterial-derived chemotactic factors such as N-formyl peptides (fMLP) and that, in the absence of PI3K neutrophils, cannot migrate toward fMLP (5, 19, 43, 44). In agreement with these previous data, the recruitment of neutrophils induced by C5a and fMLP was prevented in PI3K{gamma}–/– mice.

In conclusion, we demonstrate there is differential usage of PI3K{gamma} when neutrophil recruitment is induced by different chemoattractant molecules (CXCL1, C5a, and FMLP) and whether recruitment occurs across endothelial or epithelial barriers. Whereas C5a and fMLP appear to rely entirely on PI3K{gamma} for induction of neutrophil influx, CXCL1-mediated effects largely depend on the redundant role of PI3K{gamma} and PI3K{delta}. Neutrophil emigration across the epithelium also relies on PI3K{gamma} expression. The redundant role of and cooperation between signaling molecules and enzymes may be fundamental in providing additional specificity and complexity to signals initiated after seven-transmembrane receptor activation.


   Acknowledgments
 
We thank Valdinéria Borges and Ilma Marçal for technical assistance.


   Disclosures
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
References
 
Joel S. Hayflick is an employee of ICOS Corporation and Christian Rommel and Thomas Ruckle are employees of Merck Serono. These companies have commercial interest in developing PI3K-selective inhibitors for the treatment of inflammatory disease. The other authors declare no financial conflict of interest.


   Footnotes
 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil), Fundação de Amparo a Pesquisas do Estado de Minas Gerais (Brazil). Back

2 Address correspondence and reprint requests to Dr. Vanessa Pinho, Imunofarmacologia, Departamento de Bioquímica e Imunologia, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antonio Carlos, 6627 Pampulha, 31270-901, Belo Horizonte, MG, Brazil. E-mail address: vpinho{at}icb.ufmg.br Back

3 Abbreviations used in this paper: PtdIns, phosphatidylinositol; GPCR, G protein-coupled receptor; WT, wild type; i.pl., intrapleural; BAL, bronchoalveolar lavage; MPO, myeloperoxidase. Back

Received for publication March 27, 2007. Accepted for publication September 20, 2007.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 

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