Data Supplement

Files in this Data Supplement:

• Video 1 (MOV, 130 Kb)
  - Confocal and DIC images of a D10 cell interacting with an unpulsed CH27 cell: L10-GFP untreated. The D10 cell expressed L10-GFP. The frames were collected 10 s apart.
• Video 2 (MOV, 595 Kb)
  - Confocal and DIC images of a D10 cell interacting with an unpulsed CH27 cell: S15-GFP untreated. The D10 cell expressed S15-GFP. The frames were collected 10 s apart.
• Video 3 (MOV, 1.19 Mb)
  - Confocal and DIC images of a D10 cell interacting with an unpulsed CH27 cell: L10-GFP and filipin treated. The D10 cell expressed L10-GFP, and was pretreated with filipin. The frames were collected 10 s apart.
• Video 4 (MOV, 973 Kb)
  - Confocal and DIC images of a D10 cell interacting with an Ag-pulsed CH27 cell. The D10 cell expressed L10-GFP and the CH27 was pulsed with conalbumin. The frames were collected 10 s apart.
• Video 5 (MOV, 972 Kb)
  - DIC, confocal and Ca2+ imaging of a D10 cell undergoing stimulation by an Ag-pulsed CH27 B cell. The D10 cell was double-labeled with L10-GFP and Indo-1. The frames were collected 10 s apart.
• Video 6 (MOV, 755 Kb)
  - Confocal and DIC images of a D10 cell interacting with an Ag-pulsed CH27 cell: filipin treated. The D10 cell expressed L10-GFP and was pretreated with filipin. The CH27 was pulsed with conalbumin. The frames were collected 10 s apart.