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The Journal of Immunology, 2007, 179, 665 -672
Copyright © 2007 by The American Association of Immunologists, Inc.
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Fullerene Nanomaterials Inhibit the Allergic Response1

John J. Ryan*, Henry R. Bateman{ddagger}, Alex Stover{ddagger}, Greg Gomez{ddagger}, Sarah K. Norton*, Wei Zhao{dagger}, Lawrence B. Schwartz{ddagger}, Robert Lenk§ and Christopher L. Kepley2,{ddagger}

* Department of Biology, [s] {dagger} Department of Pediatrics, and [s] {ddagger} Department of Internal Medicine, Virginia Commonwealth University Health Systems, Richmond, VA 23294; and § Luna Innovations, Danville, VA 24541


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Fullerenes are a class of novel carbon allotropes that may have practical applications in biotechnology and medicine. Human mast cells (MC) and peripheral blood basophils are critical cells involved in the initiation and propagation of several inflammatory conditions, mainly type I hypersensitivity. We report an unanticipated role of fullerenes as a negative regulator of allergic mediator release that suppresses Ag-driven type I hypersensitivity. Human MC and peripheral blood basophils exhibited a significant inhibition of IgE dependent mediator release when preincubated with C60 fullerenes. Protein microarray demonstrated that inhibition of mediator release involves profound reductions in the activation of signaling molecules involved in mediator release and oxidative stress. Follow-up studies demonstrated that the tyrosine phosphorylation of Syk was dramatically inhibited in Ag-challenged cells first incubated with fullerenes. In addition, fullerene preincubation significantly inhibited IgE-induced elevation in cytoplasmic reactive oxygen species levels. Furthermore, fullerenes prevented the in vivo release of histamine and drop in core body temperature in vivo using a MC-dependent model of anaphylaxis. These findings identify a new biological function for fullerenes and may represent a novel way to control MC-dependent diseases including asthma, inflammatory arthritis, heart disease, and multiple sclerosis.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Nanotechnology, the use of nanomaterials at the molecular level, is a multidisciplinary scientific field undergoing exponential growth and has broad applications among all divisions of science (1, 2, 3). One form of nanomaterials, fullerenes, are soccer ball-shaped, carbon cages (C60) that can be functionalized and derivatized with a wide array of molecules (3, 4). Given their unique structure, inertness, and characteristic stability, fullerenes are being investigated as a novel way to improve upon modalities used to diagnose, monitor, and treat certain conditions.

Type I hypersensitivity is the result of B cell-produced, specific IgE Ab to common, normally innocuous Ags. The allergen-specific IgE sensitizes mast cells (MC)3 and peripheral blood basophils (PBB). Re-exposure to the allergen triggers an allergic response through the release of inflammatory mediators from MC and PBB. Indeed, many allergy medications are aimed at neutralizing (antihistamines, H1-receptor blockers) or preventing (anti-IgE; Omalizumab) this response.

No studies have examined the effects that C60 fullerenes have on the MC and PBB allergic responses. Previous studies in other cellular systems demonstrated that these molecules can inhibit various cellular pathways. We used human skin and lung MC, PBB, and an in vivo model for MC-dependent anaphylaxis to investigate the effects these molecules have on the allergic response.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 Disclosures
 References
 
Reagents

Water-soluble fullerene derivatives (polyhydroxy C60, N-ethyl-polyamino C60) referred to as "poly" or "N-ethyl" were obtained from BuckyUSA. Stem cell factor was acquired from BD Biosciences. The human IgE anti-NP Ab (used for in vitro studies) was purified from the JW8 cell line. Mouse anti-DNP IgE (used for in vivo studies) was obtained from Dan Conrad (Virginia Commonwealth University Health Systems, Richmond, VA). Anti-fullerene Abs (5) were a gift from Bernard F. Erlanger (Columbia University, New York, NY). All Western blotting Abs were purchased from Cell Signalling.

Skin MC and PBB

Human skin/lung MC and PBB were purified and cultured as described (6, 7, 8). The majority of the experiments were performed using human MC. Skin MC cultures were maintained for up to 2 mo and were ~100% skin MC. Lung MC were used within 1 wk after purification from tissue. PBB obtained from buffy coats were purified to >85%. All studies were approved by the Human Studies Committee at Virginia Commonwealth University Health Systems. For some experiments, bone marrow-derived MC from mice were obtained as described (9).

MC and PBB viability

PBS was added to water-soluble fullerenes to achieve a final concentration of 10 mg/ml. Skin MC and PBBs were incubated with varying concentrations up to 72 h. Cells were washed, collected, and stained with trypan blue (0.04%) for assessing viability. Cell counting was accomplished with an improved Neubauer hemocytometer.

Activation of skin MC and PBB

Human skin MC and PBB were sensitized with anti-NP IgE Abs (50 ng/ml for at least 24 h). All experiments were performed in duplicate or triplicate. Varying concentrations (0.001–1000 ng/ml) of fullerenes were added to the cells in minimal medium. After 24 h, the cells were centrifuged and 100 µl of medium was removed for beta-hexosaminidase and histamine measurement. The cells were washed and exposed to 100 ng/ml NP-BSA (Biosearch Technologies) with 50 µg/ml soybean tissue inhibitor in X-Vivo medium. In some experiments, the anti-Fc{epsilon}RI Ab (3B4) was used to activate non-IgE-sensitized cells. After incubation at 37°C for 30 min, the cells were centrifuged and 100 µl of medium was removed for degranulation and PGD2 assessment using a kit from Cayman Chemical. The cells were suspended in the remaining medium and placed back in an incubator. After 24 h, the cells were centrifuged and 50 µl of medium was removed for cytokine (GM-CSF, TNF-{alpha}, IL-13) measurement as described (7).

Kinex Ab array

The Kinex Ab microarray service tracks the phosphorylation (with phospho-site-specific antibodies) of ~600 proteins in dye-labeled cell lysates. For these experiments, cells were activated with or without prior incubation with 10 ng/ml poly C60 or N-ethyl C60 under the same conditions where maximal inhibition of mediator release was observed. Cells treated under the four conditions were lysed and cell lysates prepared according to the company protocol. Complete quantitation information for all detected spots was analyzed and data presented as the percentage of change from control, which measures of the change in normalized signal intensity averages between the treated sample and the control sample. Data was filtered such that only those signals that were inhibited greater than 50- and 100-fold are presented. All data are normalized to correct for systematic errors such as the difference in total protein amount between the control and experimental samples.

Measurement of reactive oxygen species (ROS) by FACS

ROS production was measured using dichlorofluorescein (DCF), a cell-permeant indicator that becomes fluorescent upon oxidation by H2O2. Skin MC or PBB were sensitized, exposed to fullerenes, and activated as above. After washing, cells were resuspended in X-Vivo medium containing 5 µM DCF and incubated at 37°C for 45 min. Cells were washed twice with skin MC medium, suspended in PBS and the mean fluorescence measured by FACS analysis. The data are presented as the mean intensity fluorescence in which the negative control (DMSO only; see below) was adjusted to approximately the same value (125–150 mean intensity fluorescence) for each experiment. All experiments were performed in duplicate.

Immunohistochemical staining of fullerenes

Skin MC were incubated with different concentrations of fullerenes at various time intervals (4, 8, and 24 h). Cytospins were made and each slide fixed in methanol/acetone/formalin (19:19:4) for 5 min and methanol/hydrogen peroxide (40:1) for 30 min. After washing in Tris-buffered saline with 0.2% Tween (TTBS) three times, the cells were placed in blocking buffer (TTBS with 1% BSA, human serum, and goat serum) for 1 h. The slides were washed three times in TTBS, and 200 µl of an Ab specific for fullerenes (5) was added to each slide. After washing the slides as before, peroxidase conjugated goat anti-mouse Abs was added. After washing, the substrate 3-amino-9-ethyl-carbazole was added and the reaction visualized and photographed with a Zeiss microscope with an Olympus digital camera.

Mouse model of anaphylaxis

Mice (C57BL/6, aged 16–20 wk) were injected i.p. with 50 µg of anti-DNP IgE or PBS. Mice then received 50 ng of poly C60 fullerene in 100 µl of PBS. Twenty four hours later, mice were challenged with an i.p. injection of 100 µg of DNP-albumin (Sigma-Aldrich) in 100 µl of PBS. Body temperature measurements were recorded with a digital rectal thermometer every 10 min for 30 min. Peripheral blood was harvested by cardiac puncture 30 min after Ag injection, and serum histamine measurements were determined by ELISA.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 Disclosures
 References
 
No studies have examined the effects fullerenes have on human MC and basophils. Thus, we first tested the effects fullerene derivatives have on cell growth and viability of skin MC and PBB. Cells incubated with up to 1000 ng/ml either polyhydroxy C60 or N-ethyl C60 for up to 72 h did not demonstrate any significant changes in cell numbers or viability (data not shown). These results suggest fullerenes are not toxic to human MC and PBB.

We next investigated the effects of fullerenes on MC and PBB Ag-induced mediator release. NP-IgE-sensitized MC and PBB were incubated with varying concentrations of fullerenes before challenge with NP-BSA. Fullerenes alone did not induce MC or PBB mediator release (data shown not shown). However, when cells were incubated with water-soluble fullerenes before challenge with optimal concentrations of Ag (100 ng/ml) there was a significant inhibition of both degranulation (Fig. 1, A and C) and cytokine production (Fig. 1, B and D) compared with skin MC and PBB not incubated with the fullerenes. Similar inhibition was observed when cells were challenged with anti-IgE receptor Abs (Fig. 1E).


Figure 1
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  		FIGURE 1. The effects of fullerenes on skin MC and PBB. Skin MC (A and B) or PBB (C and D) were sensitized with NP-IgE. Fullerenes (white, poly C60; striped, N-ethyl-C60) were added for 24 h at the indicated concentrations. For comparison, equal number of cells did not receive C60 (indicated by the (–)). The cells were washed and activated with or without (spontaneous; spon) optimal concentrations of NP-BSA (100 ng/ml) for 0.5 (degranulation) or 30 h (cytokine production), centrifuged and the supernatants examined for mediator release. In F, MC or PBB were incubated with or without the indicated concentrations of fullerenes. The next day cells were washed and activated with optimal concentrations of 3B4 and the supernatants assayed for degranulation. The results represent the average release with each condition and the SD. *, statistically significant changes in mediator levels comparing the treated cells with the untreated cells (–) using the Student t test (p < 0.05). All experiments were performed at least four times with separate donors.

 
MC found in the skin are phenotypically different from those found in the lung. For this reason we investigated the effects fullerenes had on freshly isolated human lung MC mediator release. As seen in Fig. 2, A and B, lung MC also exhibited inhibited IgE responses when cells were first exposed to fullerenes compared with cells not treated with fullerenes. The inhibitory effect was also observed from non-IgE/Fc{epsilon}RI-mediated stimulation using calcium ionophore and substance P, a potent neuropeptide (Fig. 2, C and D) and when PGD2 release was measured (Fig. 2E). Fullerene-treated skin MC also had inhibited PGD2 release compared with nontreated and IgE-activated cells (data not shown).


Figure 2
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  		FIGURE 2. A and B, The effects of fullerenes on lung MC. Lung MC (73–86% purity) were cultured with fullerenes (10 ng/ml) overnight. The next day the cells were washed and activated with or without (spontaneous; spon) the indicated concentrations of the anti-Fc{epsilon}RI-{alpha} Ab 3B4 for 0.5 (degranulation; A) or 30 h (cytokine production; B), centrifuged and the supernatants examined for mediator release. *, Significant changes (p < 0.05). C and D, The effects of fullerenes on non-IgE-mediated degranulation Cells were cultured with (white, poly C60; striped, N-ethyl C60) or without (black) the fullerenes at the indicated concentrations. Cells were washed and activated with or without (spontaneous; spon) A23187 (1000 ng/ml; C) or substance P (100 µM/ml; D) for 30 min and the supernatants examined for mediator release. *, Statistically significant changes (p < 0.05). E, The effects of fullerenes on PGD2 release. DNP-IgE-sensitized lung MCs were cultured with (white, poly C60; striped, N-ethyl C60) or without (black) fullerenes at the indicated concentrations. Cells were washed and activated with or without (spontaneous; spon) anti-Fc{epsilon}RI-{alpha} Ab 3B4 (3 µg/ml) for 30 min, centrifuged and the supernatants examined for PGD2. *, Statistically significant changes in mediator levels (p < 0.05). All experiments above were performed at least three times with separate donors.

 
We next investigated the effect fullerenes had on IgE binding to MC and levels of Fc{epsilon}RI expression on MC because the inhibitory effect observed above could be due to steric hindrance of IgE binding and/or reduced Fc{epsilon}RI expression. As seen in Fig. 3A, MC incubated with or without fullerenes demonstrated similar IgE binding. The expression levels of Fc{epsilon}RI were not changed when cells were incubated with fullerenes. In addition, fullerenes were not detected on the cell surface when cells were analyzed by FACS analysis after fullerene incubation and staining with fullerene-specific Abs (Fig. 3B). These results, along with those in Figs. 1F and 2, A and B, strongly suggest that the inhibitory effect seen with fullerene preincubation is not simply due to steric hindrance of IgE binding to Ag, IgE binding to Fc{epsilon}RI, nor to reduced Fc{epsilon}RI levels. Thus, fullerene derivatives inhibit the signaling pathways leading to human MC and PBB mediator release through mechanisms that do not involve Ag-Ab or Ab-anti-Fc{epsilon}RI receptor inhibition.


Figure 3
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  		FIGURE 3. A, Fullerenes do not inhibit IgE binding to MC or reduce Fc{epsilon}RI levels. Left, Mouse MCs were incubated with IgE for 24 h, after which fullerenes were added at the indicated concentrations (ng/ml) for 24 h. Cells were then stained with IgE plus anti-IgE, and mean fluorescence intensity was measured by flow cytometry. Right, A second group of MCs received fullerenes alone for 24 h and was then stained for surface IgE receptors using anti-Fc{epsilon}RIa. Data shown are mean ± SEM of six samples. B, Fullerenes are not detected on the cell surface under conditions that inhibit mediator release. SMC were incubated with 10 ng/ml poly (top), N-ethyl (middle), or no (bottom) fullerenes overnight. The next day, cells were washed and incubated with anti-fullerene Abs (blue), anti-Fc{epsilon}RI Abs (black), or nonspecific mouse IgG isotype-matched control Abs (red) followed by fluorescent-labeled anti-mouse F(ab')2 Abs. After washing, cells were subjected to FACS analysis.

 
To assess the cellular localization of fullerenes within human MC and PBB, we incubated these cells with water-soluble fullerenes and detected fullerenes with imunohistochemistry using an anti-fullerene Ab. As seen in Fig. 4, both skin MC and PBB incubated with the fullerenes for at least 4 h reacted with the fullerene Abs. The staining was mostly within the cytoplasm of the cells further indicating that the fullerenes exert their inhibitory affect inside the cell.


Figure 4
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  		FIGURE 4. Carbon fullerenes localize within the cytoplasm of MC and PBB. Skin MC (A–D) or PBB (E and F) were incubated with poly C60 (10 ng/ml; A, B, C and F) or N-ethyl C60 (10 ng/ml; D and F) for 4 h, washed and cytocentrifuged onto slides. The cytocentrifuge preparations were incubated with anti-C60 fullerene Abs (C–F) or with an isotype-matched control (A and B) overnight. The cytospins were washed and incubated with a 1/50 dilution of peroxidase-conjugated, anti-mouse Abs and developed with 3-amino-9-ethyl-carbazole as described. Photographs A and B are the same field taken under phase-contrast (A) or light (B) at a magnification of x400. C–F, x1000.

 
To determine the mechanisms of fullerene inhibition, we used protein microarray to provide detection and quantitation of ~600 known phospho-activated signaling molecules. As seen in Table I, ~35 Ag-induced signaling intermediates were inhibited by greater than 50-fold when cells were preincubated with the fullerenes and Ag activated compared with those nontreated and Ag activated. Of these, ~12 signaling intermediates were affected greater than 100-fold compared with Ag-only affected cells (Table I, underlined). For example, the tyrosine hydroxylase enzyme was up-regulated greater than 100-fold following IgE receptor stimulation (and was the signaling molecule up-regulated the highest by Ag stimulation). However, in cells first treated with fullerenes, this molecule was not up-regulated following Ag stimulation as detected using the Kinexus assay system. This analysis also revealed several signaling intermediates previously shown in other cellular systems to be associated with the generation of reactive oxygen species (ROS; Table I, italics).


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  		Table I. The effects of fullerenes on SMC signaling intermediates; protein array of signaling molecules affected by fullerenes in SMCa

 
Syk levels correlate with MC/PBB secretion and is a critical kinase in the Fc{epsilon}RI signaling cascade (10). The protein microarray data suggested that the tyrosine kinase Syk was inhibited greater than 50-fold following Ag stimulation when cells were first incubated with fullerenes. We performed Western blotting of Syk following Ag stimulation with or without fullerene preincubation. As seen in Fig. 5A, Syk phosphorylation was dramatically inhibited when cells were preincubated with fullerenes before activation. Similar observations were observed with Lyn and total cellular tyrosine phosphorylation (not shown) further suggesting that fullerenes inhibit MC/PBB mediator release through the reduction in Fc{epsilon}RI-associated kinase activation.


Figure 5
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  		FIGURE 5. A, Fullerenes inhibit tyrosine phosphorylation of Syk. NP-IgE-sensitized MC were incubated overnight with or without polyhydroxy-C60 or N-ethyl-polyamino-C60 (10 ng/ml). Cells were washed and activated for 10 min with or without NP-BSA (100 ng/ml). Western blotting was performed as described previously (60 ). B, Fullerenes inhibit ROS production in skin MC and PBB. Skin MC ({blacksquare}) or PBB ({blacksquare}) were sensitized overnight with NP-IgE. Cells were washed and activated for 30 min with NP-BSA (100 ng/ml) with or without preincubation with polyhydroxy-C60 (top panel) or N-ethyl-polyamino-C60 (bottom panel) at the indicated concentrations or NAC (10 mmol). Supernatants were assayed for degranulation and cellular ROS levels were detected by incubating the cells in DCF and detecting the fluorescence by FACS analysis. The results are representative of three separate MC or PBB cultures. *, Statistically significant changes in ROS levels for MC and PBB, respectively (n = 3; p < 0.05).

 
The protein microarray data also suggested that the fullerenes inhibited several molecules involved in the regulation of cellular ROS levels. We measured ROS levels in Ag-challenged cells with or without preincubation with fullerenes. As seen in Fig. 5B, preincubation of skin MC and PBB with fullerenes significantly reduced the Ag-induced increase in ROS levels within the cells. This effect was comparable to that observed with N-acetylcysteine; a powerful antioxidant. These data suggest that the inhibition of MC and PBB mediator release observed with fullerene preincubation may be due to reductions in ROS levels after Ag challenge.

Systemic anaphylaxis is predominately driven by the activation of MC and PBB through IgE-Fc{epsilon}RI cross-linking (11). We showed these cell types were inhibited by preincubation with fullerene derivatives. Thus, we hypothesized that fullerenes could inhibit MC- and PBB-induced anaphylaxis in vivo using IgE-sensitized mice. As shown in Fig. 6, animals injected i.p. with fullerenes 24 h before systemic Ag challenge had a significant decrease in the MC/PBB-derived histamine response when the animals were challenged with Ag. In addition, fullerenes injected 24 h before systemic Ag challenge significantly reduced the anaphylactic-induced drop in core body temperature. Of note, the fullerene injection did not noticeably affect the behavior in any of the mice tested (n = 35).


Figure 6
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  		FIGURE 6. Fullerenes inhibit IgE-induced anaphylaxis in vivo. Mice were sensitized with or without (black) DNP-IgE overnight. The mice were then given vehicle alone ({blacksquare}) or fullerenes ({blacktriangleup}) i.v. (50 ng/ml) overnight. The next morning the animals were challenged with DNP-BSA (100 µg/ml) and the core body temperature measured for up to 30 min. Serum histamine levels were measured by ELISA (A, 3–5 per group; n = 3 separate experiments, p < 0.05, B. n = 10 control, 13-poly-treated).

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
Disclosures
 References
 
Fullerenes or "Bucky-balls" represent the third allotrope of carbon. In this form, 60 or 70 carbon molecules are arranged in a cage structure and are water insoluble unless derivatized with various compounds. The small size of fullerenes, their inertness, and their ability to be manipulated with a host of drugs, polymers, and ligands make them attractive for the use as new therapeutics and improving the efficacy of drugs already developed. Our studies have clear implications for their use as a new way to "turn off" MC and PBB and, in turn, those disease in which these cells have a critical role.

The fullerene core can react with free radical species given its capacity to absorb electrons and disperse them through the 20 benzene rings distributed over its surface. In fact it is one of the most potent free radical scavengers known with the potential for being "sponges" in diseases involving ROS (4). This property makes them attractive therapeutic options in acute and chronic neurodegenerative diseases such as Parkinson, Alzheimer, and Lou Gehrig, which involve ROS, probably due to the over-excitation of glutamic acid receptors (12, 13, 14). To this end fullerenes derivatized with OH species have been shown to prevent ischemia (poisoning due to lack of oxygen) which is initiated and propagated through sudden increases in ROS as tissues react to energy depletion (15, 16, 17). Derivatized fullerenes also reduce ROS-induced neuronal apoptosis and have been proposed as a potential therapeutic for neurodegenerative disorders (14). Other derivatives of fullerenes include hexosulfobutyl and C3, the Tris malonate derivative, and polyethylene glycol.

The mechanism of inhibition by the fullerenes in MC and PBB appears to be, in part, through the inhibition of cellular ROS levels. As seen in Table I, fullerenes inhibited several signaling pathways previously been shown to be involved in ROS generation (18, 19, 20, 21, 22, 23, 24, 25, 26). For example inhibiting procaspase 9 prevents mitochondrial uncoupling and ROS production (27). Procaspase 9 was inhibited over 50-fold in MC incubated with fullerenes before Ag activation and could help explain the reduced ROS levels within the cells treated with fullerenes. Thus, fullerenes appear to act as an antioxidant that can absorb the free radical electrons of ROS. Our results are similar to previous studies demonstrating that fullerenes are powerful antioxidants in a variety of cell types (4).

The role of ROS in human MC and PBB-induced responses has not been investigated thoroughly. Work in rat basophil leukemic cells (RBL) has shown that stimulation through the high-affinity IgE receptor induces the production of ROS which regulate various MC responses, including degranulation, leukotriene secretion, and cytokine production (28). Conversely, antioxidants that quench intracellular ROS in rodent MC inhibit degranulation (29). Several secretogogues induced intracellular increases of ROS levels in rodent MC (30, 31, 32, 33), PBB (33), and human blood-derived MC (34). We demonstrated ROS generation in human PBB that had released significantly more mediators when challenged with diesel exhaust particles compared with nonchallenged cells (35). Some evidence indicates that allergic and inflammatory skin diseases like atopic dermatitis, urticaria and psoriasis are mediated by MC-initiated oxidative stress (36) while recent studies suggest antioxidants can reduce asthma symptoms in mice (37, 38). Previous studies have demonstrated that ROS, possibly from MC, play a significant role in the pathogenesis of a number of disorders such as inflammation, rheumatoid arthritis, asthma, psoriasis, urticaria, and contact dermatitis (39). Our studies further suggest a critical role for ROS in human MC mediator release and inhibiting ROS levels by fullerenes leads to a blunted Ag-induced response.

We found that both Lyn and Syk were inhibited by fullerene preincubation. These 2 kinases have been shown to be linked to Fc{epsilon}RI-regulated mediator release in MC and PBB (40, 41). These are the first studies linking the inhibition of tyrosine phosphorylation of these kinases to the activity of fullerenes. However, in B cells, Syk has a crucial role in intracellular signal transduction induced by oxidative stress as well as Ag receptor engagement (42). These studies found that that oxidative stress-induced Syk activation triggers the activation of several pathways, such as proapoptotic and survival pathways, and the balance among these various pathways is a key factor in determining the fate of a cell exposed to oxidative stress. Although a direct link between oxidative stress and up-regulation of Syk has not been demonstrated in MC our studies are similar to that reported in the B cell where Ag activation induces oxidative stress as well as Syk activation. The area of ROS/oxidative stress signal transduction mechanisms (secretion of ROS, ROS dependent mediator release, etc) in human MC/PBB has simply not been investigated thoroughly and represents an area of research that remains to be explored.

Given the relative infancy of the field, the studies examining the toxicity of fullerenes on biological systems are still emerging. Toxicological studies using a wide range of nanomaterials have been conflicting and inconclusive (1, 2, 43, 44, 45, 46, 47, 48). Water solubility, dose, exposure time, and similar parameters all appear to influence the cytotoxicity of the fullerenes. For example, unfunctionalized C60 appears to be cytotoxic in certain systems given its highly charged core structure but as functional side chains are added to the carbon skeleton the level of cytotoxicity appears to diminish (45, 49). Previous studies have demonstrated that fullerenes have no cytotoxic effects on keratinocytes or fibroblasts and can protect blood mononuclear cells and macrophages from oxidative stress (50, 51, 52). The level of cytotoxicity also depends on the concentration of fullerenes exposed to cells. Yamawaki showed the cytotoxic effects of hydroxyl C60 fullerenes on endothelial cells using 100 µg/ml while Chen demonstrated that polyalkylsulfonated caused renal damage with an LD50 of 600 mg/kg in rats (53, 54). In addition, high concentrations of fullerene-based amino acid nanoparticles were cytotoxic to epidermal keratinocytes, while low concentrations displayed no cytotoxic effects (55).

We show that an injection of 50 ng into mice weighing ~25 g (0.002 mg/kg) significantly inhibits anaphylaxis and does not affect the mice adversely. We have also injected five-fold higher (250 ng) with no toxicity (data not shown). Hence, the concentrations of fullerenes that are capable of inhibiting anaphylaxis and MC/PBB mediator release are 400–300,000-fold lower than the concentrations observed to have in vivo toxicity. More studies are needed to determine their effects on cell and cellular systems before they can be used as a therapeutic intervention.

In conclusion, fullerenes are capable of inhibiting MC and PBB allergic responses in vitro and anaphylaxis in vivo and suggests these molecules have previously unrecognized antiallergic properties. Fullerenes may represent a new way to control diseases largely influenced by these cells including type I hypersensitivity (anaphylaxis, hay fever, etc) (11), asthma (56), arthritis (57), multiple sclerosis (58), and other autoimmune diseases (59). For future applications, fullerenes could be modified with functional groups to specifically target their uptake to MC and PBB. For example we are investigating the effects fullerene-coated stem cell factor and IgE Fc peptide, molecules that will specifically target MC and PBB, have on mediator release.


   Disclosures
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
References
 
The authors have no financial conflict of interest.


   Footnotes
 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 This work was supported in part by National Institutes of Health Grants R01-AI27517 (to L.B.S.) and K08-AI057357 (to W.Z.) and by the Food Allergy and Anaphylaxis Network (to C.L.K.). Back

2 Address correspondence and reprint requests to Dr. Chris Kepley, PhD, MBA Department of Internal Medicine, Division of Rheumatology, Allergy and Immunology, P.O. Box 980263 MCV Station, Richmond, VA 23298. E-mail address: clkepley{at}vcu.edu Back

3 Abbreviations used in this paper: MC, mast cell; PBB, peripheral blood basophils; ROS, reactive oxygen species; DCF, dichlorofluorescein. Back

Received for publication January 26, 2007. Accepted for publication April 15, 2007.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 

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