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Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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)3 and dendritic cells (DC) and, together with the latter, have collectively been termed mononuclear phagocytes (1, 2). Accordingly, when cultured in vitro in the presence of the cytokines M-CSF or GM-CSF, monocytes can be driven to differentiate into M and DC, respectively (3, 4). Furthermore, in vivo studies also provide evidence that blood monocytes can act as precursors of M (1, 5, 6). More recent reports have shown that monocytes can under inflammatory conditions differentiate in vivo into conventional CD11chigh DC (cDC) (7, 8) and Langerhans cells (9). However, interestingly, blood monocytes seem not to contribute to the generation of splenic cDC (10, 11, 12).
Monocytes are, however, not a homogeneous cell population, but rather comprise at least two discrete subsets. Human monocytes consist of a CD142+CD16– population, which is CCR2+CD62L+CX3CR1int, and a CD14+CD16+ subset, which can be further characterized as being CX3CR1highCCR2–CD62L– (8, 13, 14). In vitro culture and expression analysis of the human monocyte subsets suggest a particular role of CD14+CD16+ monocytes in inflammatory settings (15). More recently, monocyte dichotomy has also been established in mice and rats (8, 16, 17). Circulating murine CD115+ monocytes encompass two main Gr1highCX3CR1int and Gr1lowCX3CR1high subsets (8, 16), which based on their chemokine receptor expression correlate to human CD14+/+CD16– and CD14+CD16+ monocytes, respectively (2, 8). Results of adoptive transfers of fractionated murine monocytes suggest that these cells are also functionally distinct: Gr1low monocytes were found to be recruited to resting tissues, whereas the Gr1high monocytes shuttle between the blood and the bone marrow (BM) unless recruited to sites of inflammation (8, 12). With regard to their differential fates, Gr1high monocytes were shown in mice to differentiate into cDC and Langerhans cells under inflammation (8, 9), and both Gr1high and Gr1low rat monocytes were reported to give rise to intestinal DC in steady state (17). However, for neither of the subsets the in vivo potential to become M has been investigated. Furthermore, monocyte fate studies are complicated by the recent finding that Gr1high monocytes can convert in vivo into Gr1low monocytes (12, 18, 19). In addition, a comprehensive evaluation of the in vivo differentiation potential of monocytes has to consider that the monocyte fate is likely to be dictated by the tissue environment encountered upon their extravasation. Comparison of the differentiation potential of Gr1high and Gr1low monocytes therefore requires that the two subsets will be exposed to the same microenvironment and studied side by side.
Lymphoid and nonlymphoid organs often harbor tissue-specific mononuclear phagocyte members. In this study, we investigate the differentiation potential of adoptively transferred fractionated blood monocyte subsets into DC and M, focusing on the pulmonary mononuclear phagocyte system as a nonlymphoid tissue model. The lung hosts well-defined M and DC populations, which are believed to play opposing roles in the initiation and maintenance of lung inflammations (20, 21, 22). Importantly, expression of the 
integrin CD11c discriminates both of these cell types from undifferentiated CD11c– monocytes found in this tissue (21, 23). Collectively, the pulmonary mononuclear phagocyte system is therefore particularly suited for a comparative monocyte differentiation study into either DC or M.
In this study, we show that under both inflammatory and noninflammatory conditions, Gr1highCX3CR1int and Gr1lowCX3CR1high monocytes give rise to pulmonary DC. In contrast, only Gr1low, but not Gr1high monocytes harbor the immediate potential to differentiate into lung M.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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This study involved the use of the C57BL/6 mouse strains CD11c: Diphtheria toxin (DTx) receptor (DTR) transgenic mice (B6.FVB-Tg(Itgax-DTR/GFP)57Lan/J; The Jackson Laboratory) that carry a human DTR transgene under the murine CD11c promotor (24); CX3CR1GFP mice harboring a targeted replacement of the cx3cr1 gene by a GFP reporter (25); rag1–/– mice (B6.129S7-Rag1tm1Mom/J; The Jackson Laboratory) that lack mature lymphocytes; cd80–/–/cd86–/– mice (B6.129S4-Cd80tm1ShrCd86tm1Shr/J; The Jackson Laboratory) that lack expression of both CD80 and CD86 costimulatory molecules (26); and OT-II TCR transgenic mice (C57BL/6-Tg(TcraTcrb)425Cbn/J; The Jackson Laboratory) harboring CD4+ T cells specific for OVA (27, 28). Animals were backcrossed to mice bearing the CD45.1 allotype (B6.SJL-Ptprca Pepcb/BoyJ; The Jackson Laboratory), when indicated. The wild-type (wt) C57BL/6 mice were purchased from Harlan Teklad. All mice were maintained under specific pathogen-free conditions and handled under protocols approved by the Weizmann Institute Animal Care Committee according to international guidelines.
Cell isolations
Mice were sacrificed, and blood was collected from the main artery. For bronchoalveolar lavage (BAL), the trachea was exposed to allow insertion of a catheter, through which the lung was filled and washed four times with 1 ml of PBS without Ca2+/Mg2+. Lung parenchyma and spleens were then collected, and tissues were digested with either 4 mg/ml (lung) or 1 mg/ml (spleen) collagenase D (Roche) for 1 h at 37°C, followed by incubation with ACK buffer to lyse erythrocytes. Following their isolation, mediastinal lymph nodes (LNs) were passed through a mesh and cells were collected. All isolated cells were suspended in PBS supplemented with 2 mM EDTA, 0.05% sodium azide, and 1% FCS.
Flow cytometric analysis
The following fluorochrome-labeled mAbs were purchased from BD Pharmingen or eBioscience and used according to manufacturers’ protocols: PE-conjugated anti-CD11c, I-Ab, and CD115 Abs; allophycocyanin-conjugated anti-CD11c, CD11b, CD4, and Gr1 (Ly6C/G) Abs; PerCP-conjugated anti-CD11b Ab; biotin-conjugated anti-CD45.1 Ab; and allophycocyanin- and PE-conjugated streptavidin. CX3CR1 staining using the CX3CR1 ligand fractalkine (FKN) was performed, as previously described (25). Briefly, cells were incubated with a FKN-Fc fusion protein (provided by Millenium Biotherapeutics) or PBS, followed by incubation with Cy5-conjugated anti-human Fc Ab. After an intensive wash, cells were incubated with indicated Abs. Cells were analyzed on a FACSCalibur cytometer (BD Biosciences) using CellQuest software (BD Biosciences).
Cell transfers
For blood monocyte transfers, 
20 mice were sacrificed and blood was collected to obtain an average of 15 ml of blood for each experiment. Erythrocytes and neutrophils were removed by a Ficoll density gradient (Amersham). Cells were washed and exposed to biotin-conjugated anti-CD115 or anti-Gr1 Abs (eBioscience), followed by incubation with streptavidin-conjugated MACS beads (Miltenyi Biotec). Cells were then magnetically separated, according to manufacturer’s protocol. Indicated fractions were collected and i.v. injected to recipient mice. For BM monocyte transfers, cells were isolated from donor femora and tibiae and enriched for mononuclear cells on a Ficoll density gradient, followed by immunostaining with PE-conjugated anti-CD115 and allophycocyanin-conjugated anti-Gr1 Abs (eBioscience). BM monocytes were then purified by high speed sorting using FACSAria (BD Biosciences). For T cell transfers, CD4+ T cells were isolated from OT-II;CD45.1 mice by enrichment using CD4-conjugated MACS beads (Miltenyi Biotec), according to manufacturer’s protocol.
Intratracheal (i.t.) instillation
PBS (80 µl) containing either DTx (catalog 150; List Biological Laboratories), LPS (Escherichia coli 055:B5; Sigma-Aldrich catalog L4005), or OVA (Sigma-Aldrich; catalog A5503) was applied to mouse tracheae, as previously described, with modifications (29). Briefly, mice were lightly anesthetized using isoflurane and placed vertically, and their tongues were pulled out. Using a long-nasal tip, liquid was placed at trachea top and actively aspirated by the mouse. Gasping of treated mice verified liquid application to the alveolar space.
Microscopy of lung parenchyma
Lungs were filled with 2% low melting agarose (Sigma-Aldrich; catalog A0701), as previously described (30). Live tissues were cut and imaged with a Zeiss Axioskop II fluorescent microscope using Simple PCI software.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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and DC
Alveolar and lung M and DC have been defined according to discrete surface marker expression. Both M and DC express the integrin CD11c, whereas DC are further characterized as CD11b+ cells, and M are CD11b– (21, 23, 31) (Fig. 1A). In addition, lung and alveolar M, but not DC, are autofluorescent (31, 32). In this study, we show that lung M and DC also differ in their expression of the chemokine receptor CX3CR1. Thus, surface staining with an Fc fusion of the CX3CR1 ligand FKN/CX3CL1 (FKN-Fc) showed that CD11c+CD11b+ lung DC are CX3CR1 positive, whereas CD11c+CD11b– M are CX3CR1 negative (Fig. 1B). Accordingly, in CX3CR1GFP knockin mice, whose cx3cr1 gene was replaced by a GFP cassette (25), lung and BAL DC express GFP (Fig. 1, C and D), whereas lung and BAL M do not (Fig. 1C).
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are CD11c+MHC-IIlow (21, 31). Staining for MHC-II revealed that CD11c+CX3CR1/GFP+ cells also express high levels of MHC-II, whereas CD11c+CX3CR1/GFP– cells are MHC-IIlow (Fig. 1E), supporting their definition as DC and M, respectively (31). Interestingly, we recently reported the same CX3CR1 expression pattern for small intestinal lamina propria DC and M (33). CX3CR1 is therefore a reliable marker allowing discrimination of CD11c+ DC and M in the lung and alveolar space. In contrast, we have observed that CD11b expression is significantly up-regulated on lung and alveolar M under inflammation (Fig. 1F).
For the remainder of this study that investigates the differential origin of pulmonary M and DC, we therefore apply a stringent definition of the two cell types by considering CD11c+CD11b+CX3CR1/GFP+ cells (Fig 1A; R1, R3, and R6 gated cells) as lung DC, and CD11c+CD11b–CX3CR1/GFP– (Fig 1A; R1, R2, and R5 gated cells) as resting lung M. Monocytes found in the lung parenchyma have previously been characterized as CD11c–CD11b+ cells and are defined accordingly (23) (Fig. 1A; gates R1 and R4).
Blood monocytes can differentiate into lung DC in naive mice
The most direct way to study the fate of blood monocytes is arguably the adoptive transfer of these cells into recipient’s bloodstream and subsequent tracking of graft descendants. To study the monocyte differentiation potential, we isolated the cells from donor blood according to surface expression of the monocyte-specific marker CD115 (M-CSF-R) using magnetic separation. Notably, this cell population included Gr1high and Gr1low monocyte subsets (Fig. 2A), both of which express CX3CR1 (8). To distinguish between graft- and host-derived cells, donor monocytes were retrieved from blood of cx3cr1gfp/+;CD45.1 mice and transferred into congenic CD45.2 wt recipients. Graft-derived lung DC are therefore CD45.1 CX3CR1/GFP positive (Fig. 1C), whereas host DC are CD45.1 GFP negative. The identification of graft-derived CX3CR1/GFP-negative M in recipient lungs relies solely on expression of the allotypic CD45 marker: whereas graft-derived M will be CD45.1, host cells are CD45.2. Successful monocyte transfers were confirmed by detection of grafted monocytes in the recipients’ lungs (Figs. 2B and 4A), blood, and spleens (data not shown).
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outnumber DC by far in this tissue, we did, however, not detect donor-derived (CD45.1+) lung M (Fig. 2D). In addition, we could not detect graft-derived cells in recipients’ alveolar space (data not shown).
Blood monocytes can differentiate into lung DC and M in mononuclear phagocyte-depleted mouse
The failure to detect graft-derived lung M in untreated recipients could indicate that those cells do not originate from CD115+ blood monocytes. Alternatively, the long-lived respiratory M compartment might in steady state require only limited cellular input from the blood, which could be below our level of detection. To distinguish between these options, we decided to ablate lung M before the monocyte transfer.
We took advantage of CD11c:DTR transgenic mice that allow the specific depletion of CD11chigh cells (24). The i.t. DTx instillation of CD11c:DTR transgenic mice results in the ablation of CD11c+ lung mononuclear phagocytes, including DC and M (34) (Fig. 3). Depletion of endogenous pulmonary M by the DTx treatment of CD11c:DTR recipients might open otherwise closed niches to newly coming cells. Notably, in this strategy, grafted cells, which are not CD11c:DTR transgenic, are resistant to ablation.
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in recipient’s lung (Fig. 4C), suggesting that the ablation of endogenous lung M promoted the seeding of the lung with graft-derived cells. Graft- derived monocytes, DC, and M could also be detected upon perfusion of the recipients, indicating their location in the lung parenchyma (data not shown).
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. Monocyte-derived lung DC can prime naive T cells
DC are best defined by their unrivaled capacity to stimulate naive T cells (35, 36). Importantly, in the pulmonary mononuclear system, M are established suppressors of T cell activation (20, 21, 22, 37). We therefore sought to study the functionality of graft-derived lung DC by transferring monocytes into mutant mice that lack the essential costimulatory molecules CD80 and CD86, and hence are incapable of naive T cell priming (26, 38).
We first tested the ability of grafted OVA-specific TCR transgenic T cells (OT-II) (27) to respond to i.t. OVA challenge in wt and cd80–/–;cd86–/– recipient mice (Fig. 5A). Mice received an OT-II;CD45.1 CD4+ T cell graft (day 0), followed by an i.t. challenge with OVA and LPS on the 3 subsequent days (days 1–3). Seven days after the initial immunization (day 8), mediastinal LNs were isolated and analyzed for the presence of OVA-specific CD4+ T cells (CD45.1+). The levels of surviving grafted T cells in OVA/LPS-challenged cd80–/–;cd86–/– recipients were significantly lower than in OVA/LPS-challenged wt recipients and comparable to those of LPS-challenged control recipients (Fig. 5A). In conclusion, due to the absence of competent lung DC in these cd80–/–:cd86–/– mice, grafted OVA-specific TCR transgenic CD4+ T cells failed to respond to i.t. OVA challenge (Fig. 5A). We then tested the ability of wt blood monocytes to reconstitute OT-II CD4+ T cell response in cd80–/–:cd86–/– mice. One day after OT-II T cell transfer, cd80–/–;cd86–/– mice either received a monocyte graft or no graft. To exclude B cell contaminations, CD115+ blood monocytes were retrieved from cx3cr1gfp/+;rag1–/–;CD45.2 mice, which lack mature lymphocytes. Three hours before monocyte transfer, and on the 2 following days, all mice were challenged i.t. with OVA and LPS. Seven days after the initial immunization, we isolated the mediastinal LNs and analyzed them for the presence and proliferative expansion of OVA-specific CD4+ T cells (CD45.1+). As seen in Fig. 5B, monocyte-derived DC partially reconstituted the OVA-specific CD4+ T cell response. This in vivo rescue of the CD80/CD86 deficiency confirms that adoptively transferred monocytes differentiated in the recipients into bona fide lung DC that are capable of priming naive T cells.
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The adoptive transfer of blood monocytes established that this heterogeneous population includes cells that can differentiate into both lung DC and M (Fig. 4). We next decided to test whether the two Gr1highCX3CR1int and Gr1lowCX3CR1high monocyte subsets (8) differ in their potential to give rise to pulmonary mononuclear phagocytes. To this end, we fractionated blood of cx3cr1gfp/+;rag1–/–;CD45.1 donor mice by magnetic separation according to Gr1 expression (Fig. 6A) and injected the monocyte fractions into CD45.2 recipients.
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under these conditions (data not shown). Graft-derived CX3CR1/GFP+ DC could be detected in recipients that had received either Gr1high or Gr1low blood monocytes (Fig. 6B). This indicates that in steady state both monocyte subsets reach the lung and can give rise to DC.
We next examined the monocyte subset fate in recipients depleted of endogenous mononuclear phagocytes. To this end, we transferred Gr1-fractionated CX3CR1GFP CD45.1 blood cells into CD11c:DTR CD45.2 recipients that were pretreated i.t. with DTx. Also, under these conditions, we were able to detect graft-derived DC in recipients of either of the subsets (Fig. 6C). Interestingly, however, CD45.1+ graft-derived lung M were only observed in recipients of the Gr1low monocyte graft, but not in the lungs of Gr1high monocyte recipients (Fig. 6C). This suggests that Gr1low blood monocytes, but not Gr1high monocytes, have the potential to give rise to lung M under the conditions studied.
It was shown recently that endotoxin exposure accelerates replacement of pulmonary M by BM-derived cells as compared with noninflammatory conditions (39). In the DTx-induced cell ablation system, targeted cells die by apoptosis (24, 40) and their replenishment might therefore mimic noninflammatory conditions. To investigate the differentiation potential of the monocyte subsets under inflammation, we therefore transferred Gr1-fractionated CX3CR1GFP donor blood into wt recipients (CD45.2) pretreated i.t. with LPS. Both Gr1low and Gr1high monocyte subsets readily gave rise to DC (Fig. 6D). However, again only in recipients of Gr1low monocytes, we detected graft-derived lung M (Fig. 6D).
In summary, our adoptive cell transfer experiments suggest that under inflammatory and noninflammatory conditions, both Gr1high and Gr1low blood monocytes can give rise to lung DC. Importantly, in our experimental system, only Gr1low monocytes, but not the Gr1high cells, gave rise to lung M.
Upon conversion into Gr1low monocytes, Gr1high BM monocytes gain the potential to generate lung M
Recent studies have established that Gr1high monocytes are efficient precursors of Gr1low monocytes (12, 18, 19). This suggests that the failure of Gr1high blood monocytes to give rise to lung M in our system (Fig. 6, C and D) might be due to the fact that the time window between transfer and analysis (4 days) was too short for both Gr1high/Gr1low monocyte conversion and M differentiation to occur.
To directly test whether grafted Gr1high monocytes can gain the ability to give rise to M through a Gr1low monocyte intermediate, we investigated the ability of Gr1high-derived Gr1low monocytes to give rise to lung M. Isolation of cells from donor BM allows obtaining larger amounts of Gr1high monocyte as compared with the blood (0.5 x 106 cells/femur vs 0.5 x 105 cells/ml blood). We therefore isolated cells from BM of cx3cr1gfp/+;CD45.1 donor mice and purified CD115+CX3CR1/GFP+Gr1high monocytes using a high-speed cell sorter (Fig. 7A). Purified cells were then adoptively transferred to CD11c:DTR CD45.2 recipients, which were divided into two groups that were treated with DTx on different time points.
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(Fig. 7B).
A second group of mice was treated with toxin only 4 days after receiving Gr1+ BM monocyte graft. At that time, the majority of graft-derived circulating blood monocytes had converted into Gr1low cells (Fig. 7C). Interestingly, the analysis of these recipient mice 4 days after DTx treatment (day 8) revealed the presence of both graft-derived DC and M in their lungs (Fig. 7D).
Cumulatively, these results suggest that Gr1high monocytes lack the immediate potential to give rise to lung M, but can gain this function upon conversion into Gr1low monocytes.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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. Specifically, we studied the differentiation potential of two recently described murine monocyte subsets that can be differentiated according to expression of the Gr1 surface marker.
To study the differentiation potential of monocytes into lung mononuclear phagocytes, we made use of CD11c:DTR transgenic mice, which provided us with a tool to specifically ablate pulmonary CD11c+ cells, without effecting undifferentiated monocytes (24, 34) (Fig. 3). Depletion of CD11c+ cells, including M and DC, promotes the seeding of the pulmonary mononuclear phagocyte system by blood-derived cells (L. Landsman and S. Jung, manuscript in preparation). Importantly, as opposed to other systems (41), our DTx-based depletion strategy is dependent on the genetic background of the mice (24), and non-DTR transgenic donor cells are therefore resistant to ablation upon differentiation into CD11c+ cells. However, we cannot exclude that depletion of lung M impairs lung homeostasis and as such provides proinflammatory conditions.
In agreement with studies on the rat intestinal LNs (17), we show that murine monocytes can give rise to lung DC in steady state (Fig. 2). Furthermore, we demonstrate differentiation of blood monocytes into lung DC under inflammation (Fig. 6), thus extending previous reports for skin and peritoneum (7, 8, 9). In addition, these monocyte-derived lung DC were capable of reconstituting CD4+ T cell priming in an immunodeficient mouse model (Fig. 5). Importantly, we were able to show that both Gr1highCX3CR1int and Gr1lowCX3CR1high blood monocyte subsets had the potential to give rise to lung DC under both inflammatory and noninflammatory conditions (Fig. 6).
Tissue M are believed to arise from blood monocytes (1). However, direct proof for this connection is largely limited to serosal M in the peritoneal cavity (5). In this study, we provide direct evidence that blood monocytes can give rise to parenchymal lung M in M-depleted recipients and under inflammation (Figs. 4 and 5). However, interestingly, the potential to become a lung M is restricted to the Gr1lowCX3CR1high monocyte subset.
Our results suggest that Gr1highCX3CR1int and Gr1lowCX3CR1high monocyte subsets respond in the lung differently to the same environmental signal, indicating their commitment to acquire either DC or M fate. Thus, the potential to give rise to lung M was restricted to the Gr1low monocyte subset, whereas Gr1high monocytes seem destined to become lung DC. The latter can, however, gain the potential to become lung M by conversion into Gr1low monocytes (Figs. 6 and 7). Upon differentiation into Gr1lowCCR2– cells, Gr1highCCR2+ monocytes are likely to lose their ability to respond to inflammatory signals, such as MCP-1 (CCL2) (16, 42). This scenario may reflect the need for DC during inflammation, in which Gr1high monocytes migrate to site of challenge and exclusively give rise to DC. It may ensure limitation of competition on precursor cells by steady-state tasks, such as the generation of tissue M. Replenishment of the lung M population under inflammation might be accomplished by proliferative expansion of local precursors in addition to monocyte differentiation (L. Landsman and S. Jung, manuscript in preparation).
Lung DC and M play opposing roles in the initiation and maintenance of lung inflammations. Whereas DC activate T cells and thereby promote inflammation, pulmonary M suppress these processes (20, 21, 37, 43). It has therefore been suggested that the balance of these two cell types influences the progression of lung inflammation, such as asthma (44, 45). The results presented in this study highlight the differential origins of M and DC in the lung. Although lung DC can develop from both Gr1highCX3CR1int and Gr1lowCX3CR1high monocyte subsets, lung M originate from Gr1low monocytes. In-depth understanding of the origin of lung DC and M might be of value for the development of cell therapies for respiratory disorders.
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Acknowledgments |
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Disclosures |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Footnotes |
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1 This study was supported by the Minerva and the Pasteur-Weizmann Foundations. S.J. is the incumbent of the Pauline Recanati Career Development Chair and a scholar of the Benoziyo Center for Molecular Medicine. 
2 Address correspondence and reprint requests to Dr. Steffen Jung, Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel. E-mail address: s.jung{at}weizmann.ac.il
3 Abbreviations used in this paper: M, macrophage; DC, dendritic cell; BAL, bronchoalveolar lavage; BM, bone marrow; cDC, conventional CD11chigh DC; DTx, diphtheria toxin; DTR, DTx receptor; FKN, fractalkine; int, intermediate; i.t., intratracheal; LN, lymph node; wt, wild type.
Received for publication August 29, 2006. Accepted for publication December 1, 2006.
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References |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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) system studied using the colony stimulating factor-1-deficient op/op mouse. Physiol. Rev. 76: 927-947. - and -chain genes under the control of heterologous regulatory elements. Immunol. Cell Biol. 76: 34-40. [Medline]This article has been cited by other articles:
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 M. Heron, J. C. Grutters, H. van Velzen-Blad, M. Veltkamp, A. M. E. Claessen, and J. M. M. van den Bosch Increased Expression of CD16, CD69, and Very Late Antigen-1 on Blood Monocytes in Active Sarcoidosis Chest, November 1, 2008; 134(5): 1001 - 1008. [Abstract] [Full Text] [PDF]
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D. R. Engel, J. Maurer, A. P. Tittel, C. Weisheit, T. Cavlar, B. Schumak, A. Limmer, N. van Rooijen, C. Trautwein, F. Tacke, et al. CCR2 Mediates Homeostatic and Inflammatory Release of Gr1high Monocytes from the Bone Marrow, but Is Dispensable for Bladder Infiltration in Bacterial Urinary Tract Infection J. Immunol., October 15, 2008; 181(8): 5579 - 5586. [Abstract] [Full Text] [PDF]
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D. R. Getts, R. L. Terry, M. T. Getts, M. Muller, S. Rana, B. Shrestha, J. Radford, N. Van Rooijen, I. L. Campbell, and N. J.C. King Ly6c+ "inflammatory monocytes" are microglial precursors recruited in a pathogenic manner in West Nile virus encephalitis J. Exp. Med., September 29, 2008; 205(10): 2319 - 2337. [Abstract] [Full Text] [PDF]
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 A. Vallon-Eberhard, A. Makovitzki, A. Beauvais, J.-P. Latge, S. Jung, and Y. Shai Efficient Clearance of Aspergillus fumigatus in Murine Lungs by an Ultrashort Antimicrobial Lipopeptide, Palmitoyl-Lys-Ala-DAla-Lys Antimicrob. Agents Chemother., September 1, 2008; 52(9): 3118 - 3126. [Abstract] [Full Text] [PDF]
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R. A. Bem, A. W. Farnand, V. Wong, A. Koski, M. E. Rosenfeld, N. van Rooijen, C. W. Frevert, T. R. Martin, and G. Matute-Bello Depletion of resident alveolar macrophages does not prevent Fas-mediated lung injury in mice Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L314 - L325. [Abstract] [Full Text] [PDF]
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J. McGill, N. Van Rooijen, and K. L. Legge Protective influenza-specific CD8 T cell responses require interactions with dendritic cells in the lungs J. Exp. Med., July 7, 2008; 205(7): 1635 - 1646. [Abstract] [Full Text] [PDF]
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P. Lu, L. Li, K. Kuno, Y. Wu, T. Baba, Y.-y. Li, X. Zhang, and N. Mukaida Protective Roles of the Fractalkine/CX3CL1-CX3CR1 Interactions in Alkali-Induced Corneal Neovascularization through Enhanced Antiangiogenic Factor Expression J. Immunol., March 15, 2008; 180(6): 4283 - 4291. [Abstract] [Full Text] [PDF]
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C. Jakubzick, F. Tacke, F. Ginhoux, A. J. Wagers, N. van Rooijen, M. Mack, M. Merad, and G. J. Randolph Blood Monocyte Subsets Differentially Give Rise to CD103+ and CD103- Pulmonary Dendritic Cell Populations J. Immunol., March 1, 2008; 180(5): 3019 - 3027. [Abstract] [Full Text] [PDF]
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 K. Taut, C. Winter, D. E. Briles, J. C. Paton, J. W. Christman, R. Maus, R. Baumann, T. Welte, and U. A. Maus Macrophage Turnover Kinetics in the Lungs of Mice Infected with Streptococcus pneumoniae Am. J. Respir. Cell Mol. Biol., January 1, 2008; 38(1): 105 - 113. [Abstract] [Full Text] [PDF]
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C. A Beamer and A. Holian Antigen-Presenting Cell Population Dynamics during Murine Silicosis Am. J. Respir. Cell Mol. Biol., December 1, 2007; 37(6): 729 - 738. [Abstract] [Full Text] [PDF]
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L. Landsman and S. Jung Lung Macrophages Serve as Obligatory Intermediate between Blood Monocytes and Alveolar Macrophages J. Immunol., September 15, 2007; 179(6): 3488 - 3494. [Abstract] [Full Text] [PDF]
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E. Z. Kincaid, A. J. Wolf, L. Desvignes, S. Mahapatra, D. C. Crick, P. J. Brennan, M. S. Pavelka Jr., and J. D. Ernst Codominance of TLR2-Dependent and TLR2-Independent Modulation of MHC Class II in Mycobacterium tuberculosis Infection In Vivo J. Immunol., September 1, 2007; 179(5): 3187 - 3195. [Abstract] [Full Text] [PDF]
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A. J. Wolf, B. Linas, G. J. Trevejo-Nunez, E. Kincaid, T. Tamura, K. Takatsu, and J. D. Ernst Mycobacterium tuberculosis Infects Dendritic Cells with High Frequency and Impairs Their Function In Vivo J. Immunol., August 15, 2007; 179(4): 2509 - 2519. [Abstract] [Full Text] [PDF]
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 M. Hollifield, E. B. Ghanem, W. J. S. de Villiers, and B. A. Garvy Scavenger Receptor A Dampens Induction of Inflammation in Response to the Fungal Pathogen Pneumocystis carinii Infect. Immun., August 1, 2007; 75(8): 3999 - 4005. [Abstract] [Full Text] [PDF]
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 D. Strauss-Ayali, S. M. Conrad, and D. M. Mosser Monocyte subpopulations and their differentiation patterns during infection J. Leukoc. Biol., August 1, 2007; 82(2): 244 - 252. [Abstract] [Full Text] [PDF]
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A. Cleret, A. Quesnel-Hellmann, A. Vallon-Eberhard, B. Verrier, S. Jung, D. Vidal, J. Mathieu, and J.-N. Tournier Lung Dendritic Cells Rapidly Mediate Anthrax Spore Entry through the Pulmonary Route J. Immunol., June 15, 2007; 178(12): 7994 - 8001. [Abstract] [Full Text] [PDF]
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