Data Supplement

Files in this Data Supplement:

• Video 1 (MOV, 2.58 Mb)
  - Movement of lysosomes in LysoTracker-loaded HL60 cells phagocytosing an IgG-opsonized yeast particle. HL60 cells were incubated for 30 minutes at 37℃ with buffer, i.e. non-transduced, while adhering to the bottoms of DeltaT dishes. During the last 15 minutes of incubation, LysoTracker® Red DND-99 was present. The cells were washed, and equilibrated with KRG at 37℃ after mounting the dish in the temperature setting unit of a confocal microscope. After adding IgG-opsonized yeast particles, phagocytosing cells were identified and images were captured every fourth second. The position of each yeast particle was localized by phase contrast microscopy before and after scanning the series. The video show a representative cell from 20 recorded cells per treatment obtained at two independent experiments. The phagosome is indicated with an asterisk.
• Video 2 (MOV, 1.45 Mb)
  - Movement of lysosomes in TAT-V12Cdc42-transduced, LysoTracker-loaded HL60 cells phagocytosing an IgG-opsonized yeast particle. HL60 cells were incubated for 30 minutes at 37℃ with 200nM TAT-V12Cdc42, while adhering to the bottoms of DeltaT dishes. During the last 15 minutes of incubation, LysoTracker® Red DND-99 was present. The cells were washed, and equilibrated with KRG at 37℃ after mounting the dish in the temperature setting unit of a confocal microscope. After adding IgG-opsonized yeast particles, phagocytosing cells were identified and images were captured every fourth second. The position of each yeast particle was localized by phase contrast microscopy before and after scanning the series. The video show a representative cell from 20 recorded cells per treatment obtained at two independent experiments. The phagosome is indicated with an asterisk.
• Video 3 (MOV, 2.34 Mb)
  - Movement of lysosomes in TAT-N17Cdc42-transduced, LysoTracker-loaded HL60 cells phagocytosing an IgG-opsonized yeast particle. HL60 cells were incubated for 30 minutes at 37℃ with 200nM TAT-N17Cdc42, while adhering to the bottoms of DeltaT dishes. During the last 15 minutes of incubation, LysoTracker® Red DND-99 was present. The cells were washed, and equilibrated with KRG at 37℃ after mounting the dish in the temperature setting unit of a confocal microscope. After adding IgG-opsonized yeast particles, phagocytosing cells were identified and images were captured every fourth second. The position of each yeast particle was localized by phase contrast microscopy before and after scanning the series. The video show a representative cell from 20 recorded cells per treatment obtained at two independent experiments. The phagosome is indicated with an asterisk.