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Division of Cell Biology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT 84112
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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B-p52, Pax5, E2A, and RBP-J to CD21 sequences and NF-B-p52, Pax5, NFAT species, E2A, and RBP-J to CD23 promoter sequences. Splenic T and B cell subsets displayed constitutive binding of YY1, NF-B-p52, Pax5, and Oct-1 proteins to CD21 sequences in B cells but no specific binding of NFATc3 or Pax5 in T cells. Similarly, CD23 sequences demonstrated constitutive binding of NF-B-p52 in splenic T and B cells but only Pax5 in B cells. Of the various NFAT species, only a subset were found forming constitutive DNA/protein complexes with the CD21, CD23, and IL-2 gene sequences. Maturing B cells in the marrow possess stable Pax5 complexes on CD19, CD21, and CD23 gene promoters in the nuclei of such cells, even though only CD19 is expressed. The similarity of genetic controlling elements between the CD21 and CD23 genes does not suggest a mechanism for alternative regulation of these genes; however, separation of splenic B cell subsets into T1, T2, marginal zone (MZ), and mature follicular B cells, followed by quantitative RT-PCR, demonstrated the lack of appreciable CD23 transcripts in CD21+ MZ cells. We propose an alternative derivation of MZ cells as maturing directly from T1 cells, leaving CD23 transcriptionally inactive in that lineage of cells. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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The human and mouse CD21 genes have been scrutinized for transcriptional control domains. Both genes have been found to be controlled, via reporter assays, by two distinct regions of the gene: the proximal promoter regions and sequences within the first intron. The human CD21 gene has been shown to possess a number of functional sites, including Sp1, AP1, AP2, and E box sites proximal to the TATA box sequence, and NF-B and heterogeneous ribonucleoprotein sites further upstream (–531 and –495 relative to the transcription induction site, respectively) (13, 14, 15, 16, 17, 18, 19). The mouse CD21 promoter possesses a functional Oct-1 site (equal binding via EMSA with T and B cell nuclear extracts), a consensus Pax5 binding site (showing a B cell-specific EMSA pattern), and a conserved NF-B site (also showing equal binding via EMSA with T and B cell nuclear extracts) all within the first 280 bp of the promoter region (7, 20, 21, 22). Reporter transfection assays into CD21-expressing, permissive cells with human and mouse CD21 sequences have shown the majority of the transcriptional induction activity to lie within these first few hundred base pairs of the promoter. However, the human gene also contains an inhibitory E box sequence that binds the E2A proteins E12/E47 that is proposed to limit CD21 expression (18).
Transfection of human and mouse CD21 promoter reporter constructs into T (CD21 nonexpressing) and B cell lines (expressing cells) showed virtually identical reporter expression levels (7, 13, 14, 22). However, when the first intron sequences were added to such constructs, the expression of the CD21 promoter constructs was dramatically reduced in T cells but unaltered in B cells. Analyses with the human intronic sequence localized this tissue-specific repressor element, dubbed CRS (CR2 silencer), to a single RBP-J (CBF1) binding site within the intron (14). Transfection of human K562 cells (which normally do not express CD21) with a reporter construct demonstrated silencing of such constructs only with an intact CBF1 site. The mouse first intron of the CD21 gene also possesses three similar RBP-J sites interspersed within a series of repetitive Oct1/YY1/NFAT binding sites. Elimination of single repetitive binding sites had no affect upon silencing of reporter function; however, removal of the entire set restored CD21 promoter activity in mouse T cells (22). Intriguingly, this region of the first intron is highly conserved in sequence and position between the mouse, human, and dog CD21 genes.
The expression of the CD21 gene has also been shown to be influenced by factors and conditions that alter the state of the chromatin. Demethylation of promoter sequences was shown in a variety of human B cells to be correlated with expression; inhibition of methylation induced expression in normally inactive cells (23). Additionally, increasing the level of histone acetylation in human pro-B cells and in murine T cells (via inhibiting histone deacetylase activity) induced expression of the CD21 genes (21, 23).
CD21 and the B cell-specific low-affinity IgE receptor CD23 are expressed within the same window of B cell development. CD23 has been described as possessing an IL-4-dependent enhancer element (24) with possible binding by both STAT 1 and STAT 6 (25, 26, 27). The CD23 promoter(s) have also been shown to be directly regulated by the RBP-J (Notch family), NF-B, NFAT, and Pax5 transcription factors (27, 28, 29). Similarities between transcriptional control of CD21 and CD23 exist beyond sharing the same set of transcriptional control elements. A recent report suggested that engagement of the B cell-activating factor (BAFF) receptor with its ligand was required for CD21 and CD23 expression; animals lacking BAFF showed an absence of CD21 and CD23 expression (30). The BAFF receptor is known to transduce its signal within B cells using an "alternative" activation pathway allowing for translocation of NF-B subunits into the nucleus of the cell. BAFF receptor engagement has also been linked to NFAT protein activation (31, 32).
The sequences and tissue-specific expression of the murine and human CD21 genes are highly conserved, yet clear discrepancies exist in the analyses of the two genetic elements. Much of the data generated in both systems has been derived from reporter analyses (either stable or transient transfections) and in vitro protein/DNA binding assays. To gain further insight into CD21 gene control, as well as to compare CD21 transcription with a similarly expressed gene, CD23, we initiated a series of in vivo analyses, via the chromatin immunoprecipitation (ChIP) assay, mapping DNA/protein binding complexes that form in the nucleus of native, nontransformed cells. We describe in this article constitutive, stable complexes of CD21 and CD23 sequences with NFAT family members, NF-B-p52, plus Pax5 and constituents of the Notch signaling pathway. These data lead us to propose a new model for the development of MZ B cells directly from a CD23–, T1-like cell instead of the CD23+ T2 intermediate.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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The murine CD21-expressing B cell lymphoma cell 2PK3 was obtained from American Type Culture Collection. It was maintained at 37°C/5% CO2 in RPMI 1640 (Invitrogen Life Technologies) with 10% bovine growth serum (HyClone) and 1% penicillin-streptomycin (Invitrogen Life Technologies). Splenocytes were isolated from adult BALB/c mice (National Cancer Institute) (see Figs. 2–7) and maintained in single-cell suspensions with RPMI 1640 with 10% bovine growth serum and 1% penicillin-streptomycin. Cells were treated with indicated doses of cyclosporine (CsA) (Sigma-Aldrich) or an equal volume of ethanol (EtOH) vehicle in medium for indicated times. A/J and A/WySnJ mice (see Fig. 8) were used for bone marrow and splenocyte isolation and were obtained from The Jackson Laboratory. Splenic B cells were obtained from 6- to 8-wk-old female C57BL/6 mice (see Fig. 9) purchased from the National Cancer Institute.
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Mouse monoclonal anti-YY1 (H-10), NFATc1 (7A6), NFATc2 (4G6-G5), NFATc3 (F-1), NF-B-p52 (C-5), Pax5 (A-11), CD3 (PC3/188A), rabbit polyclonal anti-Oct1 (C-21), PU.1 (T-21), and goat polyclonal anti-CD21 (M-19) Abs were purchased from Santa Cruz Biotechnology. Rabbit polyclonal anti-NFAT5 (catalog no. ab3446) was purchased from Abcam. Rabbit anti-
-actin Ab (catalog no. A2066; Sigma-Aldrich) was used as the loading control in Western blot analysis.
ChIP
This protocol was adapted from published procedures (33, 34, 35). BALB/c splenocytes were isolated, and erythrocytes were lysed using RBC lysis buffer (0.15 M NH4Cl, 10 mM KHCO3, and 0.1 mM Na2EDTA (pH 7.2)), washed, and resuspended in PBS with 0.1% BSA. A total of 3 x 106 cells was pelleted for each ChIP reaction, and PBS was removed. DNA/Protein cross-linking was done by resuspending cell pellets in 1% formaldehyde and incubated for 10 min at room temperature with rotation. Fifty microliters of 2.5 M glycine was added to each reaction and incubated for 5 min with rotation and then centrifuged to pellet cells. Pellet was washed once with 1x PBS with 0.1% BSA, and cells were resuspended in radioimmunoprecipitation assay (RIPA) (50 mM NaCl, 25 mM Tris (pH 7.5), 1 mM EDTA, 0.1% SDS, 1% w/v deoxycholate, 1% Nonidet P-40, 1% BSA, and protease inhibitor "Complete Minipill" (Roche)) and incubated for 20 min on ice with intermittent hard vortexing. Lysed samples were sonicated seven times at power setting 4 for 30 s each time. Sonicate was centrifuged for 10 min at 14,000 rpm at 4°C. Supernatant was taken and centrifuged again to pellet debris. For the input sample, 100 µl of the supernatant (named Crude Input) was mixed with 300 µl of ChIP elution buffer (10 mM EDTA, 1% (w/v) SDS, and 50 mM Tris-HCl (pH 8.0)), and cross-linking was resolved by heating at 65°C overnight and then purified with Qiagen PCR purification columns. Four-hundred microliters of the remaining supernatant with 100 µl of RIPA, supplemented with 1 protease inhibitor mixture ("Complete Minipill"; Roche) per 10 ml of volume, was used for each immunoprecipitation reaction. Samples were precleared for 2 h at 4°C (with rotation) with sheep anti-mouse or sheep anti-rabbit IgG conjugated Dynabeads (Dynal Biotech) that were previously blocked and washed with 1x PBS with 1 mg/ml BSA.
For each immunoprecipitation reaction, 1 µg of mouse monoclonal or rabbit polyclonal Ab was used, except where indicated otherwise. Immunoprecipitations were done for 2 h rotating at 4°C. Immunoprecipitated reactions were added to sheep anti-mouse or sheep anti-rabbit IgG-conjugated Dynabeads blocked and washed with 1x PBS with 1 mg/ml BSA and incubated overnight. Dynabeads were washed, three times with RIPA, twice with RIPA with 0.1 mg/ml herring sperm DNA, twice with RIPA plus 0.1 mg/ml herring sperm DNA plus 500 mM NaCl, twice with RIPA plus 250 mM LiCl, then one alternate wash with RIPA plus 500 mM NaCl, one wash with RIPA plus 250 mM LiCl, and, finally, three washes with TE (10 mM Tris (pH 8)-1 mM EDTA). DNA was eluted from the bound beads by adding 200 µl of ChIP elution buffer to each reaction and incubating overnight at 65°C incubator with gentle shaking. Supernatant was then separated from Dynabeads, and soluble DNA was isolated. PCR amplification in the presence of [32P]dCTP was done for each sample (input, nonimmune control antisera, and immunoprecipitated samples) and water control using primers specific for CD21, CD23, and CD19 promoter (and CD21 intronic) elements, -actin intragenic region and nidogen (NIDO) for 30 cycles with 6-s elongation, and 55°C annealing temperature (36). Primer sequences are listed in Table I. PCR products were subjected to electrophoresis within a sequencing gel, and the gel was dried and exposed to x-ray film overnight.
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Separation and analysis of splenic lymphocyte populations
For ChIP analysis, splenic B and T cell subsets were purified using Miltenyi Biotec magnetic beads, according to the manufacturer’s protocol. B220 and CD90 magnetic beads were used for labeling B and T cells, respectively. The purity of fractionated cells was confirmed by FACS analysis. For B cell subset separation, total splenocytes were isolated from 6- to 8-wk-old C57BL/6 female mice. After RBC lysis, a B cell-enriched population was obtained via B220+ magnetic bead depletion. B220+ cells were stained with Abs against CD21, CD23, and CD24 and resuspended at 5 x 106 cells/ml. Cells were sorted into various B cells subsets with the FACSVantage SE.
RNA preparation, cDNA synthesis, and RT-PCR
Total RNA from cells was isolated using the RNeasy mini kit (Qiagen) and quantified. cDNA was synthesized by mixing 2 µg of RNA, 10 µl of 5x first-strand buffer, 5 µl of 5 mM dNTP, 5 µl of 0.1 M DTT, 1 µl of 1.25 mM random primers, 2 µl of Maloney murine leukemia virus-reverse transcriptase (all were from Invitrogen Life Technologies), and water to a final volume of 50 µl. The reaction mixture was incubated at 37°C for 2 h. Two microliters of DNase-free RNase (1 mg/ml from Invitrogen Life Technologies) was then added, and the reaction mixture was incubated for additional 5 min at room temperature, followed by cDNA isolation, using the Qiagen PCR purification kit. Radioactive RT-PCR using 32P was performed as described previously (36). Quantitative real-time RT-PCR was performed as described previously (21, 37). Primer sequences for RT-PCR analysis are listed in Table I.
Western blot analysis
Splenic or bone marrow B and T cell subsets were purified as described above. Live cells were counted by trypan blue dye exclusion, and 5 x 106 cells were resuspended in 100 µl of RIPA (50 mM NaCl, 25 mM Tris (pH 7.5), 1 mM EDTA, 0.1% SDS, 1% w/v deoxycholate, and 1% Nonidet P-40) with one protease inhibitor "Complete Minipill" (Roche) and 100 µl of PMSF per 10 ml of lysis buffer. The reaction mixture was mixed and incubated 15 min on ice with intermittent vigorous agitation. Lysed cells were centrifuged at 14,000 rpm at 4°C for 10 min. The supernatants were pooled, and BCA Protein Assay (Pierce) was performed, according to the manufacturer’s protocol, to assay the total protein concentration. Fifteen micrograms of total protein was used for each Western sample. Rabbit anti-actin Ab (Sigma-Aldrich) was used as loading control. Tris-glycine SDS-PAGE gels (Invitrogen Life Technologies) were used for protein separation and transferred to membrane for Western blot analysis using standard protocols.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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The murine CD21 gene has previously been scrutinized for transcription factor binding sites via sequence scanning and EMSA analysis. These two procedures are useful in defining possible factor binding sites but do not always reflect relevant in vivo binding. Therefore, we sought to increase the stringency of our analyses by using ChIP analysis with Abs against the various candidate transcription factors.
The mouse CD21 promoter element possesses a variety of potential sites, including those for Oct-1 (defined by EMSA analysis) (38), Pax5, E-box proteins, and NF-B (by sequence scanning) (Fig. 1). Studies on the human CD21 promoter identified biding sites specific for E-box binding proteins (E2A), USF1, AP-2, and Sp1 (17, 18). Within the human/mouse-conserved sequences of the first intron of CD21, we have mapped a number of repetitive Oct-1, YY1, and NFAT binding sites by EMSA (22). Additionally, there are three conserved RBJ-J sites identified in this region by sequence scanning. EMSA analysis identified a functional RBP-J site in the first intron of the human CD21 gene (14). The CD23a promoter contains defined binding sites (from the human and mouse genes) for RBP-J, the Stat1/Stat6 IL-4 response element, Pax5, NF-B, E proteins, and NFAT family members (27, 28, 29).
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-actin genes (Table I). As shown in Fig. 2A, titrating the amount of anti-Oct-1 Ab increased the immunoprecipitation of sequences from the CD21 gene but not that of the -actin control, which does not possess Oct-1 binding sites. This data validated our previous EMSA observations of Oct-1 binding sites within the CD21 gene by showing that these are bound by Oct-1 in vivo. We had expected that moving away from the core Oct binding sites within the CD21 gene would diminish the immunoprecipitation of distal regions of the gene (our ChIP protocol generates sonicated DNA fragments of 
500–1000 bp in size). This was not the case for Oct-1 (or precipitations of the CD21 gene using other transcription factors), suggesting that the gene is highly condensed, perhaps due to its telomeric location, giving rise to large cross-linked complexes.
Similar data to that of the Oct-1 ChIP analysis were derived for NFATc3, YY1, and NF-B-p52. These data thus validate the in vivo binding of Oct-1, NFATc3, YY1, and NF-B-p52 to the CD21 gene and suggest such analyses can be used to differentially analyze various subsets of splenocytes (see below).
We expanded these ChIP assays with naive total splenocytes for additional transcription factors and analyzed the products with a different control sequence (NIDO) and sequences specific for the CD23 promoter (see Fig. 1). As shown in Fig. 3, the CD21 promoter and intronic elements were specifically precipitated with Abs specific for YY1, NF-B-p52, Pax5, NFATc3, E2A, RBP-J, and Oct-1. The CD23 promoter sequence was isolated with Abs specific for all of these transcription factors with the exception of YY1 and Oct-1. The NIDO control sequence was not precipitated by any of these Abs.
Splenic T and B cells demonstrate differential transcription factor binding to the CD21 gene
CD21 is expressed in B cells, not in splenic T cells; therefore, we sought to determine which of the transcription factors identified in Figs. 2 and 3 were specific for B cells. Splenic B and T cells were positively selected by magnetic bead sorting using anti-B220 for B cells and anti-CD90 for T cells. Greater than 90% of the selected cells were of the desired lineage as determined by FACS analysis (data not shown). Selected cells were immediately fixed, cross-linked, and used for ChIP analysis. As shown in Fig. 4A, NF-B-p52 binding to the CD21 genetic elements was evident in both T and B cell populations, whereas Pax5 binding was only evident in the B cell samples. This result illustrated differential binding patterns between T and B cells because Pax5 is not expressed by T cells (39). Fig. 4B shows that Oct-1 bound to the CD21 sequences in both T and B cells, whereas PU.1, as expected (40), demonstrated no binding in either cell type. Fig. 4C shows that YY1 also constitutively bound to the CD21 sequences in both T and B cells but not the control -actin and IL-2 gene sequences. NFATc3 showed specific binding to the CD21 gene only within the B cell samples, not the T cell subset. As a control, the IL-2 promoter sequence was analyzed for NFATc3 binding. ChIP analysis demonstrated NFATc3 binding to the IL-2 gene (whose transcription is NFAT dependent) was evident in the B cell subset but lacking in the T cell sample. These data indicate that NFATc3 is present in the nucleus of resting B cells (but not resting T cells) bound to defined NFAT binding sites (see below). Similar data for the CD23 gene were obtained from T and B cell subsets (Fig. 4D) in that NF-B-p52 and Pax5 showed constitutive binding to CD23 sequences in the nuclei of B cells but only NF-B-p52 was evident in T cell samples.
The previous figure suggested that NFATc3 is constitutively bound to the CD21 gene in B cells but is excluded from the gene in T cells. To further analyze this finding, the effect of CsA on the ChIP analyses of the CD21 and CD23 genes was performed. CsA blocks the ability of NFAT proteins to move from the cytoplasm to the nucleus; therefore, we anticipated that CsA treatment would block binding of the NFATc3 to the CD21 and CD23 genes. As shown in Fig. 5A, treatment of total splenocytes with CsA, but not the ethanol control, resulted in a loss of NFAT binding to the CD21 sequences. This analysis was expanded in Fig. 5B by comparing the effect of CsA treatment on total splenocytes with that of 2PK3 cells, a murine CD21-expressing B cell line. CsA treatment of splenocytes diminished binding of NFATc3 and blocked the binding of YY1 and Oct-1 to the gene sequences in the naive splenocytes but only blocked NFATc3 binding to the CD21 gene within the 2PK3 B cell line. Previously, we demonstrated that YY1 and Oct-1 binding to this fragment, via EMSA analysis, was diminished using CsA-treated nuclear extracts (22). These data suggest that the chromatin architecture of the CD21 gene in normal, nontransformed cells requires NFAT binding to allow occupancy of YY1 and Oct-1 to their binding sites within the CD21 gene. Binding of NFATc3 to the CD23 promoter sequence (Fig. 5C) was also blocked in the presence of CsA.
Redundant expression and chromatin binding of NF-AT family members
We focused on the NFATc3 protein in the previous assays because it is expressed in murine B cells, and the Ab reagents specific for the protein generated robust data in EMSA supershift and ChIP assays. However, others have shown that animals lacking NFATc3 demonstrate normal levels of B cell CD21 expression, indicating that the NFATc3 gene product is dispensable for CD21 transcription (41). To determine whether other members of the NFAT family are also expressed in murine B cells allowing for redundant control of the CD21 gene, NFAT transcript and protein quantification was performed on splenic T and B cells. In Fig. 6A, RT-PCR analysis clearly showed the expression of NFATc1, NFATc2, NFATc3, NFATc4, and NFAT5 in both T and B cell subsets. The accessory signaling protein NIP45 is also equally expressed between the two lymphocyte types. In Fig. 6B, the presence of NFATc1, NFATc2, and NFATc3 protein was also identified by Western blot analysis. The Ab regents specific for the NFATc4 and NFAT5 proteins could not resolve those species in either of these cell samples (data not shown). However, based on the previous transcript analysis, it is likely that these proteins are also present within T and B cells and that the Ab reagents are deficient for Western blot analysis.
ChIP analysis was performed on purified, unstimulated splenic B and T cells with a variety of anti-NFAT Abs (Fig. 7). A diverse pattern of NFAT family member binding was evident when comparing binding to the CD21, CD23, and IL-2 genetic elements. B cells demonstrated complexes with all five NFAT family members, although preferential complex formations were clearly evident. Thus, the IL-2 promoter sequence was clearly isolated with NFATc1, NFATc2, and NFATc3 reagents, whereas CD23 showed little complex formation with NFATc2. Similarly, the CD21 genomic elements were identified with a variety of the NFAT family members, including NFATc3. No NFAT complexes were found associated with the control NIDO gene.
The analysis of NFAT complexes formed in T cells was more restricted than that seen in the B cell populations. Although NFATc3 binding was robust in B cells, it was absent for all of the genes examined in the T cell populations. Instead, NFATc1, c4, and NFAT5 binding was detected with the CD21 gene, while no NFAT binding to CD23 sequences was observed. The NFATc1 and NFATc2 proteins were found in association with the IL-2 gene. As seen for the B cell samples, NFAT complexes were not found associated with the NIDO gene.
Pax5 binds the CD21 and CD23 genes independent of active transcription
Pax5 has been described as a master transcriptional activator required for B cell lineage commitment and induction of B cell-specific genes (42, 43). One of the primary targets of Pax5 is CD19, whose promoter possesses multiple Pax5 binding sites (44). Expression of CD19 is coincident with the expression of Pax5 at the pro-B stage (43). Animals deficient in Pax5 show B cell arrest at the early pro-B cell stage (45). As shown in Fig. 1, there are also Pax5 binding sites in the CD21 and CD23 promoters, yet the expression of these genes is much later in B cell maturation. Therefore, we were curious whether we could detect Pax5 binding to CD21 and CD23 sequences in marrow B cells (which do not transcribe these genes) or whether Pax5 was blocked from binding to these inactive genes.
One problem in analyzing mature mouse marrow is that it not only possesses maturing B cell precursors but also mature B cells that recirculate from the spleen and other sites of activation. Thus, FACS analysis of normal, mature bone marrow (Fig. 8A, top two quadrants) shows a small percentage of CD21- and CD23-expressing cells (3% of total cells). Animals lacking a functional BAFF receptor (the A/WySnJ strain) (46) produce normal B cells to the splenic T2 stage where they arrest development and differentiation. Thus, mature marrow cells from A/WySnJ mice possess normal immature B cell populations but lack recirculating CD21/CD23 positive mature B cells (Fig. 8A, bottom two quadrants).
B220+ B cells, representing immature and mature B cell populations, were obtained from the marrow and spleen of the A/J mouse and A/WySnJ mice and evaluated for the possession of the Pax5 protein by Western blot analysis. As shown (Fig. 8B), comparable levels of Pax5 protein is evident in marrow and spleen samples from the A/J and A/WySnJ mouse. As controls, YY1 protein is also evident in cell subsets from both animals, but CD21 is primarily found in the A/J splenic sample, which is consistent with the defective BAFF receptor phenotype of A/WySnJ splenic B cells.
ChIP analysis was performed on the bone marrow cells and splenocytes from the A/J and A/WySnJ mouse for Pax5 binding to the CD19, CD21, and CD23 genes. Nonspecific binding to NIDO was subtracted from each sample (see Materials and Methods). The fold enrichment of Pax5 binding vs nonspecific binding was very similar for the different samples examined, regardless of the gene. Thus, CD19, which is expressed in marrow and spleen, showed, as expected, specific binding of Pax5 to the CD19 promoter in all three samples. If the Pax5 protein was excluded from binding to the inactive CD21 and CD23 genes in the immature marrow B cells, then no specific binding should have been evident in marrow samples compared with the splenic samples, which was not the case. Therefore, the CD21 and CD23 promoters are accessible to Pax5 binding, even though the genes are transcriptionally silent. The relative fold precipitation of the spleen samples is higher than the marrow for the three genes because a higher percentage of cells in the spleen express Pax5 than in the marrow.
The CD21 and CD23 transcription profiles in spleen B cell subsets
The data in this article and that described by others suggests that CD21 and CD23 share many common transcriptional control regulators. However, the CD21 and CD23 proteins vary in the level of surface expression during peripheral B cell development. During B cell maturation, the CD21 and CD23 proteins are first found on cells making the T1 to T2 transition in the spleen (9, 47). T2 cells express both CD21 and CD23. The CD21 proteins are expressed on the FM B cells and, to an elevated level, on the surface of MZ cells. The CD23 product is expressed on the surface of T2 cells and FM B cells but not by the MZ population (9).
The CD23 protein, found on the surface as a homotrimer, is known to be cleaved from the cell by the ADAM10 protease (48); therefore, the MZ cells could conceivably lose CD23 from the cell surface by elevated expression of ADAM10.
Previously, it had been shown that CD23 transcripts are depressed in MZ cells compared with FM and T2 CD23 levels (49) using a semiquantitative RT-PCR analysis. We chose to reanalyze this question using real-time quantitative RT-PCR for CD19, CD21, CD23, and ADAM10 transcripts in sorted T1, T2, MZ, and FM cell populations. Splenic B cells were enriched with B220+ magnetic beads and stained with Abs specific for CD21, CD23, and CD24 (Fig. 9). CD23-positive (the R5 population) and CD23-negative cells (the R2 population) were then analyzed for relative expression of CD21 and CD24 and sorted (9, 47). The CD23–CD21+, CD24high cells were identified as MZ cells (R3), whereas the CD23–CD21–CD24+ cells were sorted as T1 cells (R4). The CD23+ cells were similarly sorted into the CD21+, CD24low population (FM cells, R6) and the CD21+, CD24high population (T2 cells, R7). Total RNA was obtained from these cell populations and analyzed by real-time quantitative RT-PCR (based on -actin equivalence).
Transcript analysis of the four B cell subsets indicated that CD21 transcription was highest in the MZ and T2 cells, less for the FM cells, and the least in the T1 immature B cells. These data match that of the cell surface expression levels. The CD23 transcripts also matched the cell surface staining data in that the T1 and MZ cells had very low levels of transcripts whereas T2 and FM cells had roughly equivalent levels. CD19 was abundantly expressed in all four splenic B cell types as was the ADAM10 gene. These data confirm that CD23 transcript levels are depressed in MZ cells and that the absence of CD23 on the surface of MZ cells is due to the absence of CD23 mRNA, not heightened ADAM10 production. Thus, even though the CD21 and CD23 genes share a number of key regulatory mechanisms, the stage-specific transcriptional control of these two genes is distinct.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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The ChIP assay defined constitutive binding of NFAT and NF-B species to the CD21 and CD23 (and IL-2) genetic control regions. These families of transcription factors are notable for their cytoplasmic localization until cellular activation. However, we observe nuclear binding complexes of NF-B-p52 and NFAT proteins in the absence of specific activation. One argument to explain our findings could be that the spleen consists of mixed populations of activated and unactivated cells, and the sensitivity of the ChIP procedure allows for the detection of a small subset of activated cells. We were very careful in these ChIP analyses to prevent cellular activation. Fresh cells were isolated and immediately cross-linked with formaldehyde or quickly purified and formaldehyde treated. Because the same results were obtained from purified cell populations or total splenocytes, it is not likely that our purification schemes resulted in cellular activation. If we were identifying DNA/protein complexes from a small subset of activated cells then, for example, we should have observed NFATc3 binding to gene sequences in the T cell populations because activated T cells clearly translocate NFATc3 into the nucleus, and CD21, CD23, and IL-2 gene sequences are recognized by that protein. Because this was not observed in this report, the simplest interpretation of our data is that there are low levels of different NF-B and NFAT species that are constitutively present within the nucleus of resting splenic B and T cells, and these proteins can establish binding complexes on target genes.
Investigations into the role of the NFAT proteins in the regulation of CD5 expression may provide clues pertaining to CD21 and CD23 regulation. The expression of CD5 is clearly up-regulated by NFAT activation following calcium flux (via cross-linking the BCR-BCR) due, in part, to the presence of multiple NFAT binding sites in the promoter of the gene (50, 51, 52, 53). Normal B-1a cells, however, constitutively express CD5 without requiring exogenous activation. We have previously shown that the expression of the CD21 gene is sensitive to CsA treatment (thus blocking NFAT protein migration into the nucleus) (22). However, the expression of CD21 on B cell surfaces is not increased following BCR-mediated activation (our unpublished data). One difference between CD5 and CD21 is that NFAT binding sites of the former gene are within the promoter while those in CD21 are within the intronic enhancer/silencer.
Whether the NFAT proteins are directly facilitating transcriptional induction (or repression) at the CD21 intronic site or are altering the organization state of the chromatin by recruiting histone modification enzymes is not known (7). Our previous observation that CD21 transcriptional control is influenced by the state of histone acetylation (21) plus our data in this report showing the exclusion of YY1 and Oct-1 proteins to the CD21 gene following CsA treatment in splenocytes (Fig. 5) may suggest a role for the NFAT binding proteins to alter chromatin structure in the CD21 gene. A low level of constitutive NFAT movement into the nucleus of T and B cells may be enough to satisfy chromosomal organization duties, but large-scale transcriptional induction of NFAT target genes would require the translocation of the cytoplasmic stores of the proteins.
Animals deficient in NFATc2 and NFATc3 express CD21 and CD23 on the surface of splenic B cells (41). MZ B cells are reduced in such animals based on the selective loss of CD21highIgMhigh cells. In addition, animals lacking NFATc1 express normal levels of CD21 and CD23 (54). In contrast, animals deficient in NFATc1 show an absence of CD5+ B cell populations (but those deficient in NFATc2 possess normal CD5 expression), suggesting that the NFATc1 protein is either directly responsible for constitutive CD5 expression or the outgrowth of the B-1a cells capable of expressing that surface protein (54). We demonstrate in this article that multiple NFAT family members do bind the intronic NFAT binding sites of the CD21 and CD23 genes and thus may functionally complement different family members. Indeed, our data showing expression of all five NFAT family members in splenic B cells suggest that, to accurately define the role of NFAT family members via mouse knockout models, strains may need to be developed lacking B cell expression of all of the family members (thus the genetic equivalent of CsA treatment).
The binding of the B cell-specific transcription factor Pax5 to the CD21 and CD23 promoters could help define the B cell-specific expression of these genes. CD19 has been defined as relying on Pax5 for B cell stage-specific expression, yet CD19 is expressed much earlier in the B cell lineage than CD21 and CD23 (42, 44). As shown in Fig. 8, Pax5 binding to the CD21 and CD23 genes was as evident in immature marrow cells not transcribing those genes as it was to the CD19 gene, which is being transcribed. This finding suggests that either additional positive regulatory components are missing for the CD21 and CD23 genes to be activated or that Pax5-induced transcription is repressed. Because CD23 is differentially expressed in splenic B cell maturation compared with CD21 (55, 56), two distinct regulatory components must exist, one for CD21 and the other for CD23. The presence of an inhibitory protein binding to the CD21 promoter, such as the E2 complex described for the human CD21 gene (17), could mitigate the action of an active Pax5 protein in maturing marrow B cells.
It was recently reported that BAFF-dependent activation of B cells was required for the expression of CD21 and CD23 (30). The signaling pathway ascribed to BAFF is that of the NF-B pathway, requiring receptor interacting protein and NF-B-inducing kinase activation and generating the p52 subunit (31, 32). The constitutive binding of the NF-B-p52 subunit to the CD21 and CD23 genes in T and B cells (Figs. 3 and 4) (56) requires a model of low-level translocation of NF-B species into the nucleus of both cell types. We have previously shown that NF-B-p52 binds to the CD21 and CD23 genes in splenic B cells from BAFF receptor-defective animals (A/WySnJ) as the wild-type controls (A/J) (56), indicating lack of this transcription factor is not blocking CD21 and CD23 expression in A/WySnJ mouse.
The current model of B cell development in the mouse involves a linear maturation pathway of pre-T1 B cell release from the marrow and seeding of such cells into peripheral lymphatic sites such as the spleen (Fig. 10A) (47). T1 cells in the spleen possess low levels of Bcl2 and are susceptible to BCR-induced apoptosis. Our (and other’s) analysis of T1 cells suggests this group possesses two subsets, one which expresses CD23 but not CD21, a subset we have dubbed T1b (compared with T1a that lack both CD21 and CD23 expression) (55, 56). These cells then mature into the T2 subset (CD21+/CD23+). Most models suggest that the T2 cells seed both the FM B cell compartment (CD21+/CD23+) and MZ population (CD21+, CD23–). The MZ site is unique in the splenic niche in that Notch ligand signaling is critical: animals lacking B cells that can respond to Notch ligands fail to establish a MZ cell population (49, 57, 58, 59). The CD23 gene clearly possesses binding sites to positively respond to Notch ligand activation (via heterodimer binding of RBP-J and the cytoplasmic Notch binding partner, Notch intracellular fragment (NIC)) (60); thus, it is difficult to envision a transcriptional repression pathway that specifically represses CD23 expression in MZ cells while that of CD21 is elevated. Interestingly, FM cells of the spleen and peripheral blood B cells obtained from Notch2-deficient animals (compared with wild type) show a reduced mean fluorescent intensity of staining for CD21 while that for CD23 is unaltered (58).
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1 This research was funded, in part, by National Institutes of Health Grant R01 AI2458 (to J.H.W.) and National Institutes of Health Grants R01 AI-32223 and AR-43521 (to J.J.W.). 
2 Address correspondence and reprint requests to Dr. John H. Weis, Department of Pathology, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, UT 84112. E-mail address: john.weis{at}path.utah.edu
3 Abbreviations used in this paper: T1, transition 1 B cell; BAFF, B cell-activating factor; ChIP, chromatin immunoprecipitation; CsA, cyclosporine; FM, follicular mature; F-OH, formaldehyde; MZ, marginal zone; NIDO, nidogen; RIPA, radioimmunoprecipitation assay; T2, transition 2 B cell.
Received for publication May 25, 2006. Accepted for publication March 16, 2007.
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B regulates the expression of the human complement receptor 2 gene. J. Immunol. 169: 6236-6243. 
RII (CD23) gene: functional characterization of an IL-4 enhancer element. J. Immunol. 152: 3453-3466. [Abstract]
-induced factor binding to the interleukin-4-responsive element of CD23b promoter in human tonsillar mononuclear cells: role in transient up-regulation of the interleukin-4-induced CD23b mRNA. Mol. Immunol. 35: 239-247. [Medline]B and C/EBP in CD23 expression and IgE production. Int. Immunol. 10: 1529-1538. B2. Immunity 17: 515-524. [Medline]B2 in maturing B cells. Nat. Immunol. 3: 958-965. [Medline]2 integrin). J. Leukocyte Biol. 80: 383-398. This article has been cited by other articles:
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  A. Lartigue, N. Colliou, S. Calbo, A. Francois, S. Jacquot, C. Arnoult, F. Tron, D. Gilbert, and P. Musette Critical Role of TLR2 and TLR4 in Autoantibody Production and Glomerulonephritis in lpr Mutation-Induced Mouse Lupus J. Immunol., November 15, 2009; 183(10): 6207 - 6216. [Abstract] [Full Text] [PDF]
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, Precipitation with anti-Pax5 Ab; 
, precipitation with nonspecific Ab control.
