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* Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom; and
Department of Pathology, Harvard Medical School, Brigham and Women’s Hospital, Boston, MA 02115
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Abstract |
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Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Studies using mouse models of IBD have provided convincing evidence that functionally specialized populations of regulatory T cells (TR) play an important role in the control of intestinal inflammation (5). Some of the best studied are the naturally arising CD4+CD25+ TR that have been shown to prevent and even cure colitis in the T cell transfer model (6, 7, 8). In addition to their role in intestinal homeostasis, CD4+CD25+TR play a key role in dominant tolerance to self-Ags and can also impede host protective immune responses to tumors and pathogens (9, 10, 11). Recently, expression of the transcription factor FoxP3 has been shown to be a useful marker for naturally arising TR. FoxP3 plays a key functional role in CD4+CD25+TR development, as mice with natural or induced mutations in this gene lack TR and develop a fatal multiorgan inflammatory disease (12, 13, 14). Similarly, loss of function mutations in FOXP3 have been shown to be responsible for the human autoimmune and inflammatory disease, immune polyendocrine X-linked enteropathy. Diabetes and chronic intestinal inflammation with several features resembling IBD are found in nearly all patients, and gastrointestinal symptoms are typically the reason for the initial clinical presentation, providing evidence that TR also contribute to intestinal homeostasis in humans (15, 16).
Recently, it has been proposed that the inhibitory receptor CTLA-4 plays a functional role in TR activity (6, 17, 18). This receptor belongs to the same family as CD28 and binds to the same ligands, B7-1 and B7-2. CTLA-4 is up-regulated upon T cell activation, and its activity as a negative regulator of T cell responses is now well-described (19). In vitro, ligation of CTLA-4 on activated CD4+ T cells suppresses IL-2 production and limits cell cycle progression (20, 21). In vivo, blockade of CTLA-4 leads to increased T cell-mediated immunity in a number of model systems including Ag-specific responses (22), parasitic infection (23), and autoimmune disease (24, 25, 26). Manipulation of the B7:CTLA-4 pathway is also an attractive target for stimulating antitumor immunity (27, 28, 29). In a recent clinical trial, metastatic melanoma patients were treated with a humanized anti-CTLA-4 mAb in conjunction with two modified gp100 melanoma-associated Ags; this led to cancer regression in a proportion of patients (3 of 14). However, anti-CTLA-4 treatment also resulted in autoimmune disease (6 of 14) including the development of enterocolitis (30). These findings are consistent with work in animal models, demonstrate a critical role for CTLA-4 in the regulation of peripheral tolerance in humans, and give further impetus to understanding how CTLA-4 may be important for regulating tolerance to colonic Ags.
Among resting CD4+ T cells, CTLA-4 is expressed primarily by CD4+CD25+ TR, being detectable on 
50% of these cells as compared with <1% of naive CD4+CD45RBhigh cells (6, 17). The expression of CTLA-4 on TR has been linked to regulation of organ-specific autoimmune disease in vivo, and there is some evidence to suggest that CTLA-4 is required for the suppressive function of this population in vitro (17). In the T cell transfer model of colitis, administration of anti-CTLA-4 mAb to mice that received both CD4+CD45RBhigh and CD4+CD25+ populations led to development of colitis, suggesting a key role for CTLA-4 in TR-mediated control of intestinal homeostasis (6, 18). As CTLA-4 is induced on naive T cells following activation (31), anti-CTLA-4 mAb treatment may abrogate suppression indirectly via hyperactivation of colitogenic T cells or directly via effects on the CD4+CD25+ TR population. In this report, we have used CTLA-4-deficient mice and anti-CTLA-4 mAb to dissect how CTLA-4 influences the balance between effector and TR cells in the intestine.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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BALB/c wild-type (WT), B7-1/B7-2-deficient (B7-1/B7-2 knockout (KO)), and B7-1/B7-2/CTLA-4-deficient (B7-1/B7-2/CTLA-4 KO) mice were maintained in accordance with the institutional guidelines of Brigham and Women’s Hospital and Harvard Medical School (Boston, MA; accredited by the American Association of Accreditation of Laboratory Animal Care (AALAC)). C.B-17 scid mice were purchased from Taconic Farms. For some experiments, BALB/c, C.B-17 scid, BALB/c.C57B10D2.Ly9.2 congenic, BALB/c.CTLA-4-deficient (CTLA-4 KO), and BALB/c.RAG2-deficient (RAG KO) mice were maintained in specific pathogen-free conditions at the Sir William Dunn School of Pathology (University of Oxford, Oxford, U.K.) and were used at 6–10 wk of age. All procedures were conducted in accordance with the Animals (Scientific Procedures) Act 1986.
Generation of mixed bone marrow chimeras
Bone marrow isolated from 2- to 3-wk-old BALB/c.CTLA-4 KO was depleted of T cells using anti-CD4 and anti-CD8 Abs together with anti-rat coated Dynabeads (Dynal). CTLA-4 KO bone marrow was then mixed in a 1:1 ratio with bone marrow taken from BALB/c.C57B10D2.Ly9.2 mice and injected i.v. into gamma-irradiated (5.5 Gy, 550 rad) BALB/c.C57B10D2.Ly9.2 mice. Eight weeks later, T cell reconstitution was assessed by analysis of expression of the Ly9 allele in peripheral blood. For additional experiments, CTLA-4–/– TR were sorted based on expression of CD4, CD25, and Ly9.1.
Purification of CD4+ T cells
CD4+ T cells were purified from spleens using anti-mouse CD4 (clone L3T4) coated MACS beads (Miltenyi Biotec) in accordance with the manufacturer’s instructions. Alternatively, non-CD4+ cells were depleted using anti-CD8, anti-B220, anti-H-2, and anti-Mac-1 Abs, together with anti-rat coated Dynabeads (Dynal). Purified CD4+ T cells were stained with anti-mouse CD4-CyChrome (clone RM4.5; BD Biosciences), anti-mouse CD45RB-FITC (clone 16A; BD Biosciences), and anti-mouse CD25-PE (clone PC61; BD Biosciences). Subpopulations of CD4+ cells were sorted by using three-color sorting on a FACSVantage (BD Biosciences) or MoFlo (DakoCytomation) cell sorter. T cells were sorted into CD4+(CD45RBlow)CD25+ and CD4+CD45RBhigh(CD25–) subpopulations. Sorted populations were >98.5% positive on reanalysis. FACS analysis of the sorted CD4+CD45RBhigh population showed <1% FoxP3+ cells (anti-mouse FoxP3 staining set; eBioscience).
Generation of mAb used in vivo
Anti-mouse CTLA-4 mAb (clone UC10-4F10-11) (32) and anti-mouse IL-10R mAb (clone 1B1.2) (33) were purified from hybridoma supernatant by affinity chromatography and shown to contain <1.0 endotoxin units per milligram of protein. Purified hamster IgG was used as a control (Jackson ImmunoResearch Laboratories). Fab were generated using immobilized papain (Perbio) in accordance with the manufacturer’s instructions. HPLC analysis of purified Fab before use indicated that <0.5% of the material existed in a nonmonomeric form. Surface plasmon resonance was used to confirm the binding activity of the anti-CTLA-4 Fab using a BIAcore1000 instrument (see Fig. 5A). Briefly, CTLA-4.Ig (24 ng, 1399RU) was captured onto a sensor chip (CM5) using a covalently bound anti-human Ig Ab. Anti-CTLA-4 or control Fab (20 ng/µl, 5 µl/min) was then passed through the cell and binding monitored. Nonspecific binding was assessed by measuring anti-CTLA-4 Fab binding to a control cell lacking CTLA-4-Ig.
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Immune-deficient mice, either C.B-17 scid or BALB/c.RAG2 KO mice, were injected i.p. with the sorted T cell populations. No differences were observed in the induction of colitis, or the protection from colitis between experiments using scid and RAG2 KO recipients (our unpublished observations). Mice received 4 x 105 CD4+CD45RBhigh cells alone or in combination with 1 x 105 CD4+CD25+ cells. Control mice received 1 x 105 CD4+CD25+ alone. In experiments where the pathogenic population came from B7-deficient mice, only 1 x 105 CD4+CD45RBhigh cells were transferred. Following T cell transfer, some mice received anti-CTLA-4 mAb (clone UC10-4F10-11) or a control hamster IgG; 200 µg of purified IgG were injected i.p. in PBS the day after T cell reconstitution and then on alternate days for 6–8 wk. Similarly, some mice received purified anti-CTLA-4 Fab or control Fab (100 µg) daily from the day after T cell transfer for 6–8 wk. In other experiments, mice were injected with 500 µg of anti-IL-10R mAb twice a week from the day after transfer until the end of the experiment. Mice were weighed weekly and monitored for clinical signs of colitis. Mice losing in excess of 20% of initial body weight or showing signs of severe disease were sacrificed.
Histological examination
Colons were removed from mice 6–8wk after T cell reconstitution and fixed in buffered 10% formalin. Six-micrometer paraffin-embedded sections were cut and stained with H&E. Inflammation was scored in a blinded fashion, on a scale of 0–4 where a grade of 0 was given when there were no changes observed (34). Changes associated with other grades were as follows: grade 1, minimal scattered mucosal inflammatory cell infiltrates, with or without minimal epithelial hyperplasia; grade 2, mild scattered to diffuse inflammatory cell infiltrates, sometimes extending into the submucosa and associated with erosions, with mild to moderate epithelial hyperplasia and mild to moderate mucin depletion from goblet cells; grade 3, moderate inflammatory cell infiltrates that were sometimes transmural, with moderate to severe epithelial hyperplasia and mucin depletion; grade 4, marked inflammatory cell infiltrates that were often transmural and associated with crypt abscesses and occasional ulceration, with marked epithelial hyperplasia, mucin depletion and loss of intestinal glands.
Immunofluorescence
Tissue samples were snap-frozen, cryosectioned and fixed using acetone. Sections were blocked with donkey serum (Sigma-Aldrich) and then stained with biotinylated anti-mouse CD3 (clone 145-2C11; BD Biosciences) plus streptavidin-Cy5 (Jackson ImmunoResearch Laboratories). FoxP3 staining was performed using rabbit polyclonal anti-mouse FoxP3 Abs (generously provided by F. Ramsdell, Zymogenetics, Seattle, WA) and donkey anti-rabbit IgG FITC (Jackson ImmunoResearch Laboratories). The specificity of FoxP3 staining was confirmed by the absence of nuclear staining in organs from FoxP3–/– mice (62).
Statistical analysis
Colitis scores were compared using the Mann-Whitney U test and differences were considered statistically significant with p < 0.05.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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It has been previously shown that administration of anti-CTLA-4 is able to abrogate suppression of colitis mediated by CD4+CD25+ TR in the T cell transfer model of colitis (6, 18). To dissect the mechanism by which anti-CTLA-4 Ab administration results in a loss of immune regulation, CD4+ T cell populations were isolated from CTLA-4-deficient mice and analyzed for their ability to inhibit colitis. Due to the aberrant T cell activation, lymphoproliferation and early mortality that occurs in CTLA-4-deficient mice, it was not possible to use these mice as a source of T cells for transfer experiments (35, 36). Therefore, the CTLA-4-deficient mice used in this study were maintained on a B7-1/B7-2 KO background. The absence of B7-1/B7-2 expression prevents ligation of CD28, which has been shown to be critical for activation of naive T cells, and the lymphoproliferative phenotype is avoided (37). In this respect, the B7-1/B7-2CTLA-4 KO mouse strain provides a unique tool to analyze the function of CTLA-4 on both regulatory and colitogenic T cells during the development of colitis.
To confirm that CD25+ TR can be generated in the absence of CTLA-4, splenocytes were isolated from WT, B7-1/B7-2 KO, and B7-1/B7-2/CTLA-4 KO mice and stained for expression of CD4, CD25, and FoxP3. In WT BALB/c mice, CD4+CD25+ cells comprise around 10% of the CD4+ T cell population (Fig. 1). FoxP3, a more definitive marker of TR is expressed by 15% of CD4+ cells, with the majority of CD25+ cells also expressing FoxP3 (Fig. 1). In contrast, in both B7-1/B7-2 KO and B7-1/B7-2/CTLA-4 KO mice, only 1–2% of CD4+ cells express CD25 (Fig. 1). In the same way the frequency of FoxP3+ cells is significantly reduced (2.5–3.0% of CD4+ cells) but again the majority of CD4+CD25+ cells still express FoxP3, confirming the suitability of CD25 as a TR marker in these mice. The reduction in CD4+CD25+ TR frequency in B7-1/B7-2 KO mice is most likely due to the lack of CD28 ligation, as previous studies have shown that blockade of B7-1/B7-2 or loss of CD28 resulted in a reduction in both thymic and peripheral CD4+CD25+TR (38, 39, 40).
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We next determined whether CD4+CD25+ TR from CTLA-4-deficient mice retain functional activity. This would not directly exclude a role of CTLA-4 on WT TR, as genetically modified mice often develop alternative mechanisms to compensate for the loss of a key molecule. To check for CD4+CD25+ TR function, this population was isolated from WT, B7-1/B7-2 KO, and B7-1/B7-2/CTLA-4 KO mice and transferred alone or in combination with WT CD4+CD45RBhigh cells to SCID mice. None of the isolated CD25+ populations were pathogenic, since transfer of WT, or B7-1/B7-2 KO, or B7-1/B7-2/CTLA-4 KO CD4+CD25+ cells alone to SCID recipients did not elicit any colonic inflammation (Table I). As previously described, WT CD25+ TR inhibited the development of colitis when cotransferred with WT CD4+CD45RBhigh cells (Table I, Fig. 2) (6). Similarly, CD4+CD25+ TR from both B7-1/B7-2 KO and B7-1/B7-2/CTLA-4 KO mice were able to protect mice from the induction of colitis by WT CD4+CD45RBhigh. Reducing the number of CD4+CD25+ T cells transferred failed to reveal any difference in the potency of these populations (data not shown). Thus, CTLA-4-deficient CD4+CD25+ cells retain the ability to prevent disease.
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Both WT and B7-1/B7-2 KO CD4+CD25+TR were able to inhibit colitis when cotransferred with B7-1/B7-2/CTLA-4 KO CD4+CD45RBhigh cells (Table I, Fig. 2). Similarly, B7-1/B7-2/CTLA-4 KO CD4+CD25+ cells could mediate protection from colitis induced by B7-1/B7-2 KO CD45RBhigh cells. However, when CTLA-4 was absent on both colitogenic and regulatory cells the majority of recipient mice went on to develop disease (Table I, Fig. 2). This would suggest that CTLA-4 expressed by both the colitogenic T cells and by the TR has an impact on the protection from colitis. This is consistent with multiple roles for CTLA-4 in both the activation of effector T cells and in mediating TR activity.
B7-sufficient CTLA-4 KO TR retain the ability to prevent colitis
The absence of B7.1/B7.2 has profound effects on peripheral T cell homeostasis, and may alter the activity of the CD4+CD25+ population taken from B7.1/B7.2/CTLA-4 KO mice. To rule out any effect related to the lack of B7 molecules, mixed bone marrow chimeras were generated using CTLA-4 KO (Ly9.1+) and BALB/c.C57B10D2.Ly9.2 congenic donors. As has been reported previously (41), these animals do not develop the lymphoproliferative pathology that is characteristic of intact CTLA-4 KO mice. CTLA-4 KO CD4+CD25+ TR could be recovered from these mice using expression of the congenic marker Ly9.1. The sorted CTLA-4 KO CD4+CD25+ TR contained a similar frequency of FoxP3+ cells as the counterpart WT CD4+CD25+ TR and were also able to prevent colitis induced by transfer of WT CD4+CD45RBhigh cells to BALB/c.RAG2 KO mice (Fig. 3). This confirms our observation that TR that cannot use CTLA-4 are still able to prevent colitis, irrespective of expression of B7.
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Anti-CTLA-4 mAb treatment targets CD4+CD25+ TR and not colitogenic T cells to abrogate suppression
We have previously reported that administration of anti-CTLA-4 mAb abrogates CD4+CD25+ TR-mediated suppression of colitis (6, 18). However, whether the Ab functions via effects on effector T cells, TR, or both is not known. To investigate this issue, the outcome of anti-CTLA-4 mAb administration was examined in transfer experiments where expression of CTLA-4 was restricted to the colitogenic or TR cells. Suppression of colitis mediated by B7-1/B7-2/CTLA-4 KO CD4+CD25+ TR was not affected by anti-CTLA4 mAb treatment (Fig. 4A). In addition, while WT CD4+CD25+ TR were able to prevent colitis induced by CD4+CD45RBhigh cells from B7-1/B7-2/CTLA-4 KO mice, the addition of anti-CTLA-4 mAb led to a loss of protection and the development of disease (Fig. 4B). Together, these data indicate that anti-CTLA-4 mAb abrogates TR-mediated control of colitis via its effects on TR and not colitogenic effector cells.
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Next, we investigated the possibility that the anti-CTLA-4 treatment was somehow eliminating the TR population. Anti-CTLA-4 mAb bound to the surface of TR might lead to deletion of this population, or it might cross-link CTLA-4 providing an agonistic signal, inhibiting TR expansion. To explore these possibilities, anti-CTLA-4 Fab were generated and used for in vivo studies (Fig. 5A). Administration of anti-CTLA-4 Fab to SCID mice cotransferred with B7-1/B7-2/CTLA-4 KO CD4+CD45RBhigh cells and WT CD4+CD25+ cells led to a loss of suppression of colitis similar to that seen in mice injected with intact anti-CTLA-4 mAb (Fig. 5B). Protection from colitis was not affected in similarly transferred mice that received a control Fab. These results indicate that the functional effects of anti-CTLA-4 administration are independent of the Fc portion of the Ab, ruling out Ab-induced cross-linking of CTLA-4 and generation of an agonistic signal, as well as Fc-mediated cellular depletion, as mechanisms of action.
Accumulation of CD4+CD25+TR is not inhibited by the presence of anti-CTLA-4
It was possible that blockade of CTLA-4 on TR inhibited a positive signal required for TR proliferation and accumulation. To assess the effect of the mAb on TR accumulation, expression of an allotypic marker was used to distinguish the progeny of the CD4+CD25+ and CD4+CD45RBhigh populations following transfer. CD4+CD25+ TR were purified from WT BALB/c (Ly9.1) mice and transferred together with CD4+CD45RBhigh cells from BALB/c.C57B10D2.Ly9.2 congenic mice to immunodeficient recipients. After 2 wk, CD4+ T cells from the spleen were analyzed for expression of allotypic markers. Mice that received anti-CTLA-4 mAb had an increased number of total CD4+ T cells, with both Ly9.1+ and Ly9.2+ cells being increased 2-fold (Fig. 6A, data not shown). However, the proportion of CD4+CD25+ progeny (Ly9.1+CD4+ cells) vs CD4+CD45RBhigh progeny was similar in mice that had received anti-CTLA-4 to that seen in control mice, a pattern that was maintained at later time points (Fig. 6B). These data show that the ability of anti-CTLA-4 to suppress TR activity is not the result of impaired accumulation of the CD4+CD25+population.
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It remained possible that the presence of anti-CTLA-4 was in some way able to disrupt the homing of CD4+CD25+ TR. To determine whether this was the case, the GALT from T cell-transferred SCID mice was analyzed for the presence of TR. Frozen mesenteric lymph node sections from mice that had received CD4+CD45RBhigh cells in combination with CD4+CD25+ cells and anti-CTLA-4 mAb were stained for the presence of CD3+ cells. The same sections were also stained for the presence of FoxP3+ cells, a definitive marker of TR (Fig. 7A). In all mice analyzed, it was possible to identify FoxP3+CD3+ cells in the mesenteric lymph node (MLN), even though these mice had ongoing colitis. In addition, FACS analysis was performed to quantify FoxP3 expressing cells in spleen and MLN of these mice (Fig. 7B). FoxP3+CD4+ T cells could be found in both the spleen and MLN of T cell transferred mice, with no significant reduction in the frequency of FoxP3+ cells in anti-CTLA-4-treated mice compared with the controls (Fig. 7C). Together, these data demonstrate that FoxP3+CD4+ T cells are able to access the GALT in anti-CTLA-4-treated mice and yet fail to control the colitogenic T cell response.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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In this study, we have used T cells lacking CTLA-4 as a tool to investigate the role of CTLA-4 in CD4+CD25+ TR function and to clarify the effects of the anti-CTLA-4 mAb. Mice deficient in B7-1 and B7-2 as well as CTLA-4 were used as donors (37), thus avoiding the problems associated with isolating CD4+CD25+ T cells from CTLA-4-deficient mice (35, 36). Previous studies have shown that administration of anti-CTLA-4 mAb overcame the ability of CD4+CD25+ TR to protect from colitis (6, 18). Here, we demonstrate that protection is dependent upon CTLA-4 expression by TR but is independent of CTLA-4 expression by the colitogenic CD4+CD45RBhigh population, indicating that the effects of the Ab are mediated through CTLA-4 expressed on the CD4+CD25+ TR.
The mode of action of the anti-CTLA-4 mAb was analyzed by comparing the activity of intact IgG and Fab. Fab were as effective as intact Ab, indicating that the Ab does not cause Fc-mediated deletion of the TR; nor does it cross-link CTLA-4 providing an agonistic signal. Ligation of CTLA-4 has been reported to inhibit activation induced cell death in certain T cell populations (48, 49); it was thus possible that the effect of the mAb was due to the inhibition of a similar survival signal. However, anti-CTLA-4 did not reduce the accumulation of TR progeny following transfer in vivo. Instead, absolute numbers of CD25+ TR were increased along with splenomegaly, although the frequency of TR progeny as a percentage of the transferred CD4+ cells remained similar. Comparable results have been observed in a clinical trial in humans, where anti-CTLA-4 treatment, despite inducing antitumor responses and autoimmunity, did not reduce the frequency of FoxP3 expression in PBMC (50). Furthermore, in our system, it was possible to detect the FoxP3+ progeny of transferred CD4+CD25+ TR in the GALT of anti-CTLA-4-treated mice, consistent with these cells migrating to the lymphoid organs that drain the diseased tissue but being unable to prevent the development of disease.
B7-1/B7-2/CTLA-4 KO mice contained a reduced frequency of peripheral CD4+CD25+ TR, consistent with a role for B7:CD28-mediated costimulation in the generation and maintenance of these cells (38, 39, 40). The additional deficiency in CTLA-4 did not alter the frequency CD4+CD25+ cells. Significantly, this population retained FoxP3 expression and TR function in the absence of CTLA-4, indicating that the receptor is not absolutely required for the development of CD4+CD25+ TR. Importantly, administration of the anti-CTLA-4 mAb did not overcome the regulatory activity of CTLA-4 deficient CD4+CD25+ TR, showing that ligation of CTLA-4 on the colitogenic population had limited impact on the regulation of colitis in this system.
Recent reports have shown that the interaction of CTLA-4 with B7 ligands expressed by APCs may modulate immune responses. This raises the possibility that anti-CTLA-4 mAb may disrupt TR function by preventing a CTLA-4-mediated signal through B7-1/B7-2 expressed on dendritic cells (DC). The data show that binding of a CTLA-4.Ig fusion protein to the surface of DC induces expression of indoleamine 2,3 dioxygenase, leading to the depletion of tryptophan and inhibition of T cell function (51). CTLA-4 expressed by CD25+ TR has similar effects, suggesting that this interaction may be important for the suppressive activity of these cells (52). Our findings that CD4+CD25+ TR are able to suppress colitis induced by CTLA-4-deficient CD4+CD45RBhigh cells in a CTLA-4-dependent manner are in line with that data, raising the possibility that it is ligation of B7-1/B7-2 on DC by CTLA-4 expressed by TR that is crucial for TR-mediated control of colitis. Additional experiments are required to test this hypothesis.
As not only CTLA-4, but also B7 is up-regulated on T cells upon activation, the interaction of CTLA-4 with B7 expressed on effector T cells may also play a role in CD4+CD25+ cell-mediated suppression. In a recent report, B7-deficient CD4+CD25– cells were found to be refractory to TR-mediated suppression in vitro and in vivo (53). Whether CTLA-4 expressed by TR was important in these interactions is not clear. Indeed, while this may represent another mechanism by which CD4+CD25+ TR influence T cell responses, it does not appear to be essential, since in our model B7-1/B7-2/CTLA-4 KO CD4+CD45RBhigh T cells remained susceptible to suppression by WT TR, through a CTLA-4 dependent mechanism.
PD-1, another member of the CD28/CTLA-4 family, has also been linked to TR-mediated prevention of colitis. CD4+CD25–PD-1+ T cells expressing high levels of FoxP3 and CTLA-4 have been shown to prevent colitis in the CD4+CD45RBhigh transfer model (54). This protection, like that mediated by CD4+CD25+ T cells, was overcome by anti-CTLA-4 but not by anti-PD-1 Abs. Although the role of PD-1 in TR function remains elusive, this report further highlights the functional importance of CTLA-4 in protection from colitis in a different model.
The data presented here demonstrate that signaling through CTLA-4 is required for WT CD25+ TR to exert their suppressive phenotype. However, TR generated in the absence of CTLA-4 retain the ability to suppress colitis, suggesting that they are able to compensate for the loss of this receptor. The functionality of CTLA-4-deficient CD25+ TR cells in vitro has been linked to an increased production of the immune suppressive cytokines IL-10 and TGF-
(42). In vivo, CTLA-4-deficient TR cells rely on IL-10 more heavily than the WT TR population, as administration of an anti-IL-10R mAb abrogated the protection mediated by these cells. Although CTLA-4-deficient TR can compensate for the absence of CTLA-4, this compensation is not complete. Thus, CD4+CD25+ T cells from B7-1/B7-2/CTLA-4 KO mice failed to suppress effector T cells of the same genotype, although they were effective in controlling both WT and B7-1/B7-2 KO CD4+CD45RBhigh cells. By contrast, B7-1/B7-2/CTLA-4 KO CD4+CD45RBhigh cells were susceptible to regulation by WT and B7-1/B7-2 KO CD4+CD25+ cells. One explanation could be that the CD4+CD45RBhigh population from B7.1/B7.2/CTLA-4 KO mice is less susceptible to regulation than that from WT or B7.1/B7.2 KO mice. Thus, the combination of CTLA-4 deficiency in both effector and TR populations is sufficient to tip the balance away from a regulated immune response and toward the development of inflammatory pathology. These data are consistent with a growing acceptance that immune regulation is mediated by multiple mechanisms and that removal of one or other pathway may or may not result in a loss of suppression as a function of the particular assay.
In clinical studies, anti-CTLA-4 mAb has been developed as a reagent to enhance T cell immunity (55). Early results with anti-CTLA-4 mAb in humans have suggested that this reagent may be an effective means to enhance antitumor immunity; however, the treatment has also lead to transient negative side effects, including the development of enterocolitis (30). More recent trials with the same anti-CTLA-4 mAb have seem similar autoimmune and gastrointestinal perturbances (56, 57, 58). As FoxP3 expression was not perturbed by the therapy, the effects have been ascribed to the interaction of anti-CTLA-4 mAb with effector cells (50). The data presented herein offer an alternative interpretation, indicating that anti-CTLA-4 can target CD4+CD25+ TR function without changes in FoxP3 expression.
The clinical studies illustrate that the anti-CTLA-4 mAb treatment may also have an impact on TR-mediated suppression of T cell responses and alter the balance of immune regulation, especially in the gut. Although the studies show that manipulation of CTLA-4 signaling is useful as a mechanism to enhance immune responses in a therapeutic setting, they also highlight the need to separate its useful and harmful effects. This is particularly significant at this time, with the recent approval of CTLA-4 Ig therapy for the treatment of rheumatoid arthritis by the Food and Drug Administration (59). In this article, we present a model where CTLA-4 mediates two different effects. On one hand, it reduces the pathogenicity of effector cells (60, 61). On the other, it is required for TR-mediated control of immune responses. Both roles have to be taken into account when designing clinical trials, as the stimulation of antitumor effector cells by blocking CTLA-4 could be accompanied by the breakdown of TR-mediated self-tolerance.
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Acknowledgments |
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Disclosures |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Footnotes |
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1 S.R., N.R., and F.P. were funded by the Wellcome Trust. A.I. is a recipient of a postdoctoral grant from the Spanish Ministerio de Educación y Ciencia. A.H.S. is the recipient of National Institutes of Health Grants R01 AI40614 and AI38310. 
2 Address correspondence and reprint requests to Prof. Fiona Powrie, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, U.K. E-mail address: fiona.powrie{at}path.ox.ac.uk
3 Abbreviations used in this paper: IBD, inflammatory bowel disease; TR, regulatory T cell; WT, wild type; KO, knockout; MLN, mesenteric lymph node.
Received for publication November 23, 2005. Accepted for publication July 14, 2006.
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References |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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, and CTLA4. J. Immunol. 171: 5012-5017. in CD4+CD25+ regulatory T cell function. Eur. J. Immunol. 34: 2996-3005. [Medline]This article has been cited by other articles:
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  L. E. Jeffery, F. Burke, M. Mura, Y. Zheng, O. S. Qureshi, M. Hewison, L. S. K. Walker, D. A. Lammas, K. Raza, and D. M. Sansom 1,25-Dihydroxyvitamin D3 and IL-2 Combine to Inhibit T Cell Production of Inflammatory Cytokines and Promote Development of Regulatory T Cells Expressing CTLA-4 and FoxP3 J. Immunol., November 1, 2009; 183(9): 5458 - 5467. [Abstract] [Full Text] [PDF]
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 C Coquerelle, G Oldenhove, V Acolty, J Denoeud, G Vansanten, J-M Verdebout, A Mellor, J A Bluestone, and M Moser Anti-CTLA-4 treatment induces IL-10-producing ICOS+ regulatory T cells displaying IDO-dependent anti-inflammatory properties in a mouse model of colitis Gut, October 1, 2009; 58(10): 1363 - 1373. [Abstract] [Full Text] [PDF]
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 S. Sakaguchi, K. Wing, Y. Onishi, P. Prieto-Martin, and T. Yamaguchi Regulatory T cells: how do they suppress immune responses? Int. Immunol., October 1, 2009; 21(10): 1105 - 1111. [Abstract] [Full Text] [PDF]
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Y. Tanriver, A. Martin-Fontecha, K. Ratnasothy, G. Lombardi, and R. Lechler Superantigen-Activated Regulatory T Cells Inhibit the Migration of Innate Immune Cells and the Differentiation of Naive T Cells J. Immunol., September 1, 2009; 183(5): 2946 - 2956. [Abstract] [Full Text] [PDF]
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 K. S. Peggs, S. A. Quezada, C. A. Chambers, A. J. Korman, and J. P. Allison Blockade of CTLA-4 on both effector and regulatory T cell compartments contributes to the antitumor activity of anti-CTLA-4 antibodies J. Exp. Med., August 3, 2009; 206(8): 1717 - 1725. [Abstract] [Full Text] [PDF]
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 C. Robert and F. Ghiringhelli What Is the Role of Cytotoxic T Lymphocyte-Associated Antigen 4 Blockade in Patients with Metastatic Melanoma? Oncologist, August 1, 2009; 14(8): 848 - 861. [Abstract] [Full Text] [PDF]
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N. R. Locke, S. J. Patterson, M. J. Hamilton, L. M. Sly, G. Krystal, and M. K. Levings SHIP Regulates the Reciprocal Development of T Regulatory and Th17 Cells J. Immunol., July 15, 2009; 183(2): 975 - 983. [Abstract] [Full Text] [PDF]
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 L.-F. Lu and A. Rudensky Molecular orchestration of differentiation and function of regulatory T cells Genes & Dev., June 1, 2009; 23(11): 1270 - 1282. [Abstract] [Full Text] [PDF]
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 J. Kurtz, F. Raval, C. Vallot, J. Der, and M. Sykes CTLA-4 on alloreactive CD4 T cells interacts with recipient CD80/86 to promote tolerance Blood, April 9, 2009; 113(15): 3475 - 3484. [Abstract] [Full Text] [PDF]
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 J. Verhagen, L. Gabrysova, S. Minaee, C. A. Sabatos, G. Anderson, A. H. Sharpe, and D. C. Wraith Enhanced selection of FoxP3+ T-regulatory cells protects CTLA-4-deficient mice from CNS autoimmune disease PNAS, March 3, 2009; 106(9): 3306 - 3311. [Abstract] [Full Text] [PDF]
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R. H. Friedline, D. S. Brown, H. Nguyen, H. Kornfeld, J. Lee, Y. Zhang, M. Appleby, S. D. Der, J. Kang, and C. A. Chambers CD4+ regulatory T cells require CTLA-4 for the maintenance of systemic tolerance J. Exp. Med., February 16, 2009; 206(2): 421 - 434. [Abstract] [Full Text] [PDF]
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E. M. Schmidt, C. J. Wang, G. A. Ryan, L. E. Clough, O. S. Qureshi, M. Goodall, A. K. Abbas, A. H. Sharpe, D. M. Sansom, and L. S. K. Walker CTLA-4 Controls Regulatory T Cell Peripheral Homeostasis and Is Required for Suppression of Pancreatic Islet Autoimmunity J. Immunol., January 1, 2009; 182(1): 274 - 282. [Abstract] [Full Text] [PDF]
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F. Flores-Borja, E. C. Jury, C. Mauri, and M. R. Ehrenstein Defects in CTLA-4 are associated with abnormal regulatory T cell function in rheumatoid arthritis PNAS, December 9, 2008; 105(49): 19396 - 19401. [Abstract] [Full Text] [PDF]
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 K. Watanabe, V. P. Rao, T. Poutahidis, B. H. Rickman, M. Ohtani, S. Xu, A. B. Rogers, Z. Ge, B. H. Horwitz, T. Fujioka, et al. Cytotoxic-T-Lymphocyte-Associated Antigen 4 Blockade Abrogates Protection by Regulatory T Cells in a Mouse Model of Microbially Induced Innate Immune-Driven Colitis Infect. Immun., December 1, 2008; 76(12): 5834 - 5842. [Abstract] [Full Text] [PDF]
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H.-X. Wei, Y.-H. Chuang, B. Li, H. Wei, R. Sun, Y. Moritoki, M. E. Gershwin, Z.-X. Lian, and Z. Tian CD4+CD25+ Foxp3+ Regulatory T Cells Protect against T Cell-Mediated Fulminant Hepatitis in a TGF-{beta}-Dependent Manner in Mice J. Immunol., November 15, 2008; 181(10): 7221 - 7229. [Abstract] [Full Text] [PDF]
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G. Kim, O. Turovskaya, M. Levin, F. R. Byrne, J. S. Whoriskey, J. G. McCabe, and M. Kronenberg Spontaneous Colitis Occurrence in Transgenic Mice with Altered B7-Mediated Costimulation J. Immunol., October 15, 2008; 181(8): 5278 - 5288. [Abstract] [Full Text] [PDF]
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 K. Wing, Y. Onishi, P. Prieto-Martin, T. Yamaguchi, M. Miyara, Z. Fehervari, T. Nomura, and S. Sakaguchi CTLA-4 Control over Foxp3+ Regulatory T Cell Function Science, October 10, 2008; 322(5899): 271 - 275. [Abstract] [Full Text] [PDF]
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A. Samanta, B. Li, X. Song, K. Bembas, G. Zhang, M. Katsumata, S. J. Saouaf, Q. Wang, W. W. Hancock, Y. Shen, et al. TGF-{beta} and IL-6 signals modulate chromatin binding and promoter occupancy by acetylated FOXP3 PNAS, September 16, 2008; 105(37): 14023 - 14027. [Abstract] [Full Text] [PDF]
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Y. Zheng, C. N. Manzotti, F. Burke, L. Dussably, O. Qureshi, L. S. K. Walker, and D. M. Sansom Acquisition of Suppressive Function by Activated Human CD4+CD25- T Cells Is Associated with the Expression of CTLA-4 Not FoxP3 J. Immunol., August 1, 2008; 181(3): 1683 - 1691. [Abstract] [Full Text] [PDF]
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A. L. Tang, J. R. Teijaro, M. N. Njau, S. S. Chandran, A. Azimzadeh, S. G. Nadler, D. M. Rothstein, and D. L. Farber CTLA4 Expression Is an Indicator and Regulator of Steady-State CD4+FoxP3+ T Cell Homeostasis J. Immunol., August 1, 2008; 181(3): 1806 - 1813. [Abstract] [Full Text] [PDF]
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Y. Onishi, Z. Fehervari, T. Yamaguchi, and S. Sakaguchi Foxp3+ natural regulatory T cells preferentially form aggregates on dendritic cells in vitro and actively inhibit their maturation PNAS, July 22, 2008; 105(29): 10113 - 10118. [Abstract] [Full Text] [PDF]
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F. S. Hodi, M. Butler, D. A. Oble, M. V. Seiden, F. G. Haluska, A. Kruse, S. MacRae, M. Nelson, C. Canning, I. Lowy, et al. Immunologic and clinical effects of antibody blockade of cytotoxic T lymphocyte-associated antigen 4 in previously vaccinated cancer patients PNAS, February 26, 2008; 105(8): 3005 - 3010. [Abstract] [Full Text] [PDF]
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K. Takamura, S. Fukuyama, T. Nagatake, D.-Y. Kim, A. Kawamura, H. Kawauchi, and H. Kiyono Regulatory Role of Lymphoid Chemokine CCL19 and CCL21 in the Control of Allergic Rhinitis J. Immunol., November 1, 2007; 179(9): 5897 - 5906. [Abstract] [Full Text] [PDF]
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F. Mingozzi, N. C. Hasbrouck, E. Basner-Tschakarjan, S. A. Edmonson, D. J. Hui, D. E. Sabatino, S. Zhou, J. F. Wright, H. Jiang, G. F. Pierce, et al. Modulation of tolerance to the transgene product in a nonhuman primate model of AAV-mediated gene transfer to liver Blood, October 1, 2007; 110(7): 2334 - 2341. [Abstract] [Full Text] [PDF]
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M. Guilliams, G. Oldenhove, W. Noel, M. Herin, L. Brys, P. Loi, V. Flamand, M. Moser, P. De Baetselier, and A. Beschin African Trypanosomiasis: Naturally Occurring Regulatory T Cells Favor Trypanotolerance by Limiting Pathology Associated with Sustained Type 1 Inflammation J. Immunol., September 1, 2007; 179(5): 2748 - 2757. [Abstract] [Full Text] [PDF]
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N. Singh, P. R. Chandler, Y. Seki, B. Baban, M. Takezaki, D. J. Kahler, D. H. Munn, C. P. Larsen, A. L. Mellor, and M. Iwashima Role of CD28 in fatal autoimmune disorder in scurfy mice Blood, August 15, 2007; 110(4): 1199 - 1206. [Abstract] [Full Text] [PDF]
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 A. S. Mohamood, M. L. Guler, Z. Xiao, D. Zheng, A. Hess, Y. Wang, H. Yagita, J. P. Schneck, and A. R. A. Hamad Protection from Autoimmune Diabetes and T-Cell Lymphoproliferation Induced by FasL Mutation Are Differentially Regulated and Can Be Uncoupled Pharmacologically Am. J. Pathol., July 1, 2007; 171(1): 97 - 106. [Abstract] [Full Text] [PDF]
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 S. Tuve, B.-M. Chen, Y. Liu, T.-L. Cheng, P. Toure, P. S. Sow, Q. Feng, N. Kiviat, R. Strauss, S. Ni, et al. Combination of Tumor Site-Located CTL-Associated Antigen-4 Blockade and Systemic Regulatory T-Cell Depletion Induces Tumor-Destructive Immune Responses Cancer Res., June 15, 2007; 67(12): 5929 - 5939. [Abstract] [Full Text] [PDF]
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