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Department of Epidemiology and Public Health and Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Rhigh HEC-6SThigh, is dependent on signaling through the lymphotoxin- receptor (LTR). Plasticity of PLN HEVs during immunization with oxazolone was apparent as a reversion to an immature phenotype (MAdCAM-1highPNAdlowLTRlow HEC-6STlow) followed by recovery to the mature phenotype. The recovery was dependent on B cells and was inhibited by LTR-Ig treatment. Concurrent with HEV reversion, at day 4 following oxazolone or OVA immunization, reduced accumulation of Evans blue dye and newly activated DCs in the draining LNs revealed a temporary afferent lymphatic vessel (LV) functional insufficiency. T cell priming to a second Ag was temporarily inhibited. At day 7, lymphangiogenesis peaked in both the skin and draining LN, and afferent LV function was restored at the same time as HEV phenotype recovery. This process was delayed in the absence of B cells. LV and HEV both express the LTR. During lymphangiogenesis in the draining LN, HEV, and LV were directly apposed; some vessels appeared to express both PNAd and LYVE-1. Pretreatment with LTR-Ig drastically reduced the number of PNAd+LYVE-1+ vessels, suggesting a reduction in LV and HEV cross-talk. The concordance in time and function and the close physical contact between LVs and HEVs in the remodeling process after immunization indicate that the two vascular systems are in synchrony and engage in cross-talk through B cells and LTR. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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complex, and its activity is crucial for the expression of PNAd on the HEV luminal surface during development and chronic inflammation (3, 4, 5, 6). MAdCAM-1 is expressed on HEVs of mesenteric LNs (MLNs) and Peyer’s patches in adult mice; it is only apparent in early development on HEVs of peripheral LNs (PLNs) and thus is a marker of immature HEVs in those nodes (7). HEVs also display CCL19 and CCL21, which are instrumental in encouraging the entrance of naive T cells and directing dendritic cell (DC) traffic in the LN. Lymphatic vessels, distinguished by LYVE-1 (8), play important roles in fluid regulation and immune responses. Afferent LVs allow entrance of soluble Ag and APCs into LNs and efferent LVs return lymphocytes back to the blood circulation. The effects of immunization on LVs and their functions and the consequences of these alterations have not been thoroughly investigated. LVs and HEVs are maintained in homeostasis in LNs during the steady state. Early after immunization by skin painting with oxazolone (OX), injection of sheep RBC (SRBC) in adjuvant, or bacterial or viral infection, LNs undergo remodeling, with complex kinetics of lymph flow, cell content in lymph, blood flow, and HEV gene expression. Lymph flow and lymph cell content increase soon after an initial inflammation and eventually return to a normal level (9). Blood flow and lymphocyte migration into LNs peak at 72–96 h (10, 11, 12, 13), accompanied by an increase in HEV number and dilation (14, 15). These factors account for the significant LN enlargement apparent at 72–96 h (16, 17). In contrast, during earlier times after immunization with OX or SRBC, there is an interesting pattern of HEV gene regulation that apparently contradicts LN enlargement. An initial down-regulation of genes that contribute to L-selectin ligand, including FucT-VII, GlyCAM-1, and Sgp200, is followed by a recovery, indicating HEV plasticity (18, 19). The mechanism of HEV remodeling after immunization has not been elucidated.
Data from studies in which afferent LVs are severed support the concept that LV function is necessary for HEV maintenance. After afferent LVs are severed, dramatic effects are apparent in HEVs. These include morphological changes, a decrease in the uptake of [35S]sulfate (an indication of HEC-6ST function) (20, 21), a reduction of lymphocyte adherence to the vessels (22, 23, 24), and decreased expression of PNAd, GlyCAM-1, and Fuc-TVII (19, 23, 24). However, an increase in MAdCAM-1 expression (23) suggests that these events are not simply due to a general down-regulation of blood vessel gene expression and indicate that continual accumulation of afferent lymph factor(s) in LNs is necessary for HEV maintenance. The nature of such lymph factor(s) and the signaling events that they induce are unknown.
Because LVs and HEVs are crucial for LN function, we wondered whether these two systems interact during an immune response. In this study, we present the results of studies designed to 1) characterize the effects of immunization on HEVs by quantitation of expression of genes characteristic of mature or immature HEVs; 2) identify cells and cytokines that contribute to changes in HEV gene expression after immunization; and 3) determine whether the plasticity of HEVs is due to changes in LV.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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C57BL/6, TNFR1–/–(p55–/–), and µMT mice were purchased from The Jackson Laboratory. nu/nu mice were purchased from Taconic. RAG–/– mice were obtained from Dr. R. Flavell (Yale University, New Haven, CT). All mice were studied under a protocol approved by the Yale University Institution Animal Care and Use Committee.
OX and FITC Painting and OVA immunization
OX immunization was performed as skin painting of 50 µl per LN area of 4% OX (Sigma-Aldrich) in acetone. Mice were anesthetized by injection i.p. of ketamine/xylazine. OX was applied on shaved skin on the abdomen at the inguinal LN (ILN) and brachial LN areas. In all the experiments, OX was painted only once. LNs were harvested at different time points after OX (n 
5 per time point). OX was applied on flanks for skin angiogenesis analysis. For FITC skin painting, 200 µl of 2% FITC (Sigma-Aldrich) in 1:1 (v/v) acetone/dibutylphthalate mixture was applied on shaved skin on flanks. FITC+ DCs were analyzed in draining LNs 24 h after FITC painting.
In other experiments, mice were immunized with a total of 250 µg/mouse OVA (Sigma-Aldrich) in CFA with 375 µg of Mycobacterium tuberculosis (Difco) in CFA divided between two flanks and tail.
Flow cytometry analysis
Staining for flow cytometry analysis was performed by conventional procedures using PE-conjugated Ab to CD11c (BD Pharmingen). The stained cells were subjected to flow cytometry with a FACSCalibur instrument (BD Biosciences).
Immunofluorescence and immunohistochemistry
LNs were embedded and frozen in OCT (Sakura), and 7-µm (LN) or 5-µm (skin) cryocut sections were fixed with cold acetone. Blocking buffer was either 3% BSA (Sigma-Aldrich) and 5% mouse serum (Zymed) in PBS or 5% BSA and 4% goat serum (Sigma-Aldrich) in PBS. The following Abs were used: hamster anti-mouse LTR, rat anti-mouse MAdCAM-1, rat anti-mouse PNAd MECA-79 (BD Pharmingen); rat anti-mouse LTR (Apotech); and rabbit anti-human LYVE-1 (cross-reactive with mouse LYVE-1) (Upstate Biotechnology). In one experiment, rat anti-mouse LYVE-1 (R&D Systems) was used. Rabbit anti-mouse HEC-6ST Ab was developed in our laboratory (3, 5). Biotin-conjugated secondary Abs were goat anti-rat IgG (Caltag Laboratories), goat anti-hamster IgG, goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories), and anti-mouse MHC class II (I-Ab). For immunofluorescence, Cy3-goat anti-rat IgM, Cy3-goat anti-rat IgG, and Cy2-streptavidin (Jackson ImmunoResearch Laboratories) were used as detecting reagents. Immunohistochemistry was conducted with alkaline phosphatase and alkaline phosphatase substrate kits (Vector Laboratories).
Quantitative real-time RT-PCR
PLNs collected from each individual mouse were pooled and pressed between two slides, washed with a large volume of PBS, and run through a 40-µm cell strainer (BD Falcon). The stromal material retained in cell strainer was immediately placed into lysis buffer for RNA extraction. For RNA extraction after LTR-Ig treatment, the isolated individual PLNs were placed immediately in lysis buffer for RNA extraction (RNeasy kit; Qiagen). The extracted RNA was treated with RNase-free DNase I kit (Qiagen), and cDNA was reverse transcribed using Superscript II kit (Invitrogen Life Technologies). Real-time PCR was performed in ABI Prism 7000 sequence detection system (Applied Biosystems) using SYBR Green PCR master mix (Qiagen). GAPDH cDNA was measured simultaneously in all reactions as internal control. Data were normalized based on the expression of GAPDH. Primer sequences for real-time PCR are as follows: GAPDH, (forward) 5'-CTGCACCACCAACTGCTTAG; (reverse) 5'-GATGGCATGGACTGTGGTCAT; GlyCAM-1, (forward) 5'-CCTGCCTGGGTCCAAAGATGAAC, (reverse) 5'-CTGGTGTAGCTGGTGGGAGTGGAC; MAdCAM-1, (forward) 5'-GTCCTGCACGGCCCACAACAT, (reverse) 5'-CCAGTAGCAGGGCAAAGGAGAG; CD34, (forward) 5'-AAACGGCCATTCAGCAAGACAACA, (reverse) 5'-TCGCCACCCAACCAAATCACAAG; FucTIV, (forward) 5'-TGCACCGCGTTGACCACCTTC, (reverse) 5'-AGCCGCCGCGCCCCCTAAA; FucTVII, (forward) 5'-GGACCTCCTCGGGCCACCTACG, (reverse) 5'-CGCCAAGCAAAGAAGCCACGATAA; HEC-6ST, (forward) 5'-TGCCCCACCTCCAAACAT, (reverse) GACCAACGCCACGCCTGAGA; SLC, (forward) 5'-ACTCTAAGCCTGAGCTATGTGCAA, (reverse) 5'-GGCGGCGCATCAGGT; LTR, (forward) 5'-TGGTGCTCATCCCTACCTTCA, (reverse) 5'-TTCTCTCTATCCTCTCCCCCAG.
Injection of LTR-Ig and control-Ig (LFA3-Ig)
Five-week-old C57BL/6 mice were injected i.p. once with 100 µg of either LTR-Ig or LFA3-Ig (a gift from J. Browning, Biogen Idec). PLNs were analyzed 7 days later. These experiments were performed twice with comparable results. To identify the role of LTR in HEV gene recovery, 5-wk-old mice were immunized with OX, and 4 days later injected i.p. with 100 µg of either LTR-Ig or LFA3-Ig. PLNs were analyzed 3 days after the injection (i.e., OXd7). For the role of LTR in LV and HEV cross-talk after immunization, LTR-Ig or control-Ig were injected i.p. 24 h before OX immunization. PLNs were analyzed at OXd4.
Evans blue dye accumulation in ILNs
Thirty microliters of 1% Evans blue dye (Eastman Kodak) was injected s.c. into each of the rear footpads. Dye accumulation in skin and in inguinal LNs was evaluated 6 h later.
Cell proliferation experiments
Seven days after immunization with OVA in CFA as above, draining PLN cells were isolated, and 2 x 105 cells per well were cultured with 1 mg/ml OVA for 2 days. 3[H]Thymidine (1 µCi) (Amersham Biosciences) was added to each well and cultured for an additional 20 h. All proliferation assays were performed in triplicate in 96-well flat-bottom plates (BD Biosciences), and the incorporation of 3H was evaluated by standard liquid scintillation (Wallac). Results are expressed as the stimulation index of cpm obtained with cells plus Ag divided by cpm of cells in medium alone.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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R and HEC-6ST (mature HEV markers) are down-regulated and then recover after OX; converse regulation of MAdCAM-1 (immature HEV marker)
HEV plasticity is apparent after skin painting with OX or immunization with SRBC in adjuvant (18, 19). The pattern of HEV gene regulation after Ag exposure, apparent as a decrease in the expression of some genes, but not of others, is puzzling. We adopted the OX skin painting model to analyze the plasticity that had been revealed in previous studies because OX is a standard reagent for investigating LN remodeling (25, 26). We analyzed protein and mRNA expression, concentrating on three important HEV genes: HEC-6ST, MAdCAM-1, and LTR. These genes respectively represent a mature HEV marker, an immature HEV marker, and the critical receptor in their development. PNAd expression also was evaluated as the MECA-79 epitope, but the data for this particular series of experiments are not reported in this study because the changes in the cellular pattern and intensity of staining are the product of several genes, including HEC-6ST. Furthermore, because PNAd is a product of several genes, it cannot be evaluated at the mRNA level. Mice were immunized with OX (in acetone), and axillary, brachial, and inguinal LNs (PLNs) from a minimum of five mice per time point were harvested at several different times after the single immunization and analyzed by immunohistochemistry and quantitative RT-PCR. Several changes were seen in HEVs after OX, but not after vehicle-alone (acetone) treatment. LTR and HEC-6ST were regulated in a similar pattern. An initial decrease was apparent by 2 days (OXd2, data not shown). The lowest level was seen at 4 days (OXd4; Fig. 1A, b and f). A rebound was apparent by 7 days (OXd7; Fig. 1A, c and g), with full recovery by 14 days (OXd14) (Fig. 1A, d and h). Surprisingly and importantly, in contrast with these two genes, MAdCAM-1 expression, which is normally very low in PLNs (Fig. 1Ai), increased after OX immunization. There was an increase not only in the number of MAdCAM-1-expressing vessels (from 4 ± 2 to 21 ± 7 per 7-µm LN section, apparent from counting 12 random LN sections from five mice), but also in the level of MAdCAM-1 expression on those vessels (Fig. 1A, j and k). By OXd14, MAdCAM-1 returned to the normal very low level seen in PLN HEVs (Fig. 1Al). Interestingly, at OXd7, MAdCAM-1 expression was localized to those individual high endothelial cells that expressed low HEC-6ST (Fig. 1B), consistent with a generally reciprocal relationship of these proteins as has been noted in mesenteric LN HEVs (5).
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R, HEC-6ST, and GlyCAM-1, proteins that contribute to PNAd, was decreased at OXd4 and then rebounded. MAdCAM-1 mRNA exhibited a converse pattern (Fig. 1C). Thus, HEV plasticity after immunization was evident as reversion to an immature phenotype, and then a recovery. Because HEC-6ST is a selective HEV gene and its regulation represents HEV remodeling, we use HEC-6ST as a mature HEV marker in the remainder of this communication. Neither T nor B cells are required for the initiation of HEV remodeling, but B cells are necessary for optimal HEV recovery after OX
To identify the mechanism of the changes in HEVs, we next investigated which cells and cytokines were responsible for these effects. First, we asked whether the remodeling of HEVs was the consequence of lymphocyte activity by evaluating HEV gene regulation in LNs of RAG–/– mice. Although these mice have no mature T or B cells, they exhibit HEV networks, although the level of constitutive HEC-6ST expression was considerably lower than in wild-type (WT) PLNs, and many of the vessels exhibited abluminal PNAd staining (Fig. 2A). The initial down-regulation of HEC-6ST still occurred at OXd4 (Fig. 2B, a–c). Therefore, the down-regulation of HEC-6ST after OX was not due to LN enlargement subsequent to infiltrating Ag-specific cells. However, the rebound of HEV gene expression at OXd7 was not as vigorous as in WT mice (Fig. 2B, a–c; compare with Fig. 1A, e–g), indicating a requirement for T and/or B cells for this later stage. The results indicate that the mature HEV phenotype and the optimal rebound of HEV gene expression after OX require T and/or B cells.
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Given the importance of TNFR1 in inflammation and its somewhat less prominent role in secondary lymphoid organ development, we asked whether HEV gene expression after OX also was regulated through this pathway. We investigated HEV gene expression in LNs of three TNFRI (p55) knockout mice after OX. The pattern of HEV gene regulation after OX was similar to that seen in WT mice (Fig. 2B, j–l). Therefore, the TNFR1 signal is not important in HEV gene regulation after OX.
Mature HEV phenotype recovery from OX is dependent on LTR and B cells
The critical importance of LTR signaling for HEV gene expression in development and chronic inflammation was shown in our previous experiments conducted in gene knockout and transgenic mice (3). Furthermore, HEVs express LTR (27), suggesting that the effect of LTR ligands was directly on the vessels themselves. In a recent publication, Browning et al. (29) found that long-term, repeated treatment with LTR-Ig, an inhibitory fusion protein of the LTR with human Ig Fc region (28), reduced the expression of HEV genes. In agreement with their observations, we found that by 7 days after even a single i.p. injection of 100 µg of LTR-Ig, expression of several HEV genes was dramatically down-regulated. Quantitative real-time RT-PCR revealed down-regulation of mRNAs of several HEV genes, including the posttranslational modifying enzymes HEC-6ST, FucT-IV, FucT-VII, HEV core glycoprotein, GlyCAM-1, and MAdCAM-1 (Fig. 3A). Interestingly and importantly, this treatment also resulted in 
65% reduction of the LTR itself (Fig. 3A) (n = 6) suggesting, for the first time, feedback regulation of the LTR.
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R activity. Mice were preimmunized with OX 4 days before (OXd4) and then injected i.p. with 100 µg of LTR-Ig or control LFA3-Ig. PLNs were harvested 3 days later (i.e., OXd7) and analyzed by immunofluorescent double staining for PNAd and HEC-6ST expression. The rebound of HEC-6ST expression occurred in HEVs of mice injected with LFA3-Ig (Fig. 3Bb), but not in those of mice injected with LTR-Ig (Fig. 3Be). In fact, some HEVs of LTR-Ig treated mice appeared to express only abluminal PNAd (Fig. 3B, d and e) (n = 3 mice per group). Because we had determined that B cells were crucial for the recovery of mature HEVs, we then asked whether this B cell activity was through LTR. In OXd7 LN, HEC-6SThigh HEVs were located in the interfollicular region and in isolated B cell clusters (Fig. 3Bc). After LTR-Ig treatment, some HEVs and B cells still colocalized, but HEC-6ST did not recover (Fig. 3B, e and f), suggesting that B cell-regulated HEV gene recovery is mediated through the LTR. Taken together, these data strongly support the concept that the role of the LTR ligand on HEVs is direct and crucial, not only for initiation and maintenance of HEVs, but also for the recovery of the mature phenotype. Because B cells play such an important role in HEV gene regulation, we next investigated the cell number and composition in the draining LN after immunization. There was an increase in total cell number, and interestingly, a disproportionate increase in B cells (Fig. 3C). The proportion of B cells in the draining LN drastically increased from 17.8 ± 1.1% at steady state to 49.4 ± 0.1% at day 4 and remained at 40.4 ± 3.8% at day 7.
Afferent LV function is impaired at day 4 and recovers at day 7 after OX skin painting
The above data indicate two stages of HEV plasticity after immunization: reversion to an immature phenotype and then recovery (Fig. 1). They also suggest that HEV recovery after immunization requires B cells and LTR signaling (Figs. 2 and 3). However, neither B cells nor cytokine signals explain the initiation of HEV remodeling after immunization. The early effects on HEV gene regulation after OX appeared to mimic those in previous reports of severed afferent lymphatic vessels (19, 23, 24). This caused us to wonder whether HEV phenotypic reversion after OX was due to the exclusion of factor(s) arriving through the afferent LVs.
In this study, we investigated the effect of OX treatment on the accumulation of lymph and cells arriving from afferent lymph in the draining LNs. Afferent lymph access was investigated by monitoring Evans blue dye accumulation in draining inguinal LNs (ILNs). Mice were preimmunized with OX for either 4 or 7 days (OXd4 or OXd7). Evans blue was injected s.c. into the rear footpads of these and control nontreated mice. Although Evans blue can be visualized in ILNs within 2 h and is apparent in efferent lymph within 4 h, we found that 6 h after dye injection was the optimal time point to distinguish the accumulation pattern in ILNs and the surrounding skin. Six hours after Evans blue injection, dye accumulated in the ILNs of control mice (Fig. 4A, a and d), and the surrounding skin was only slightly blue, indicating normal LV function. In contrast, much less dye accumulated in OXd4 ILNs (Fig. 4A, b and e) and, in fact, it collected in the skin around the nodes, which appeared edematous (Fig. 4Ab). This indicates an insufficiency in afferent LV function at this time point. Blood vessel angiogenesis and efferent LV dilation was apparent, consistent with the previous observations regarding blood and efferent lymph flow after immunization (Fig. 4Ab). In OXd7 mice, the amount of dye that accumulated in ILN was nearly normal (Fig. 4A, c and f) and the edema subsided, indicating that LV function had recovered (Fig. 4Ac). ILNs were sectioned and evaluated microscopically to take advantage of Evans blue autofluorescence (red). In nontreated ILNs, a dense fluorescence was apparent encircling the medullary area, the site of the highest lymphatic vessel density; the fluorescence was much fainter in the OXd4 ILNs and recovered in OXd7 ILNs (Fig. 4A, d and f) (n = 5).
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Afferent LV function, evaluated as DC access and T cell priming, is impaired at day 4 and recovers at day 7 after immunization with OX
Having shown that LV function impairment is a consequence of immunization, we next asked whether there was an immunologic consequence to the reduction in lymph access. We investigated whether the accumulation of cells arriving from afferent lymph was reduced. By FITC skin painting, the number of newly activated DC in draining LNs can be evaluated by FACS. Individual mice were preimmunized with OX at different time points (day 0, 1, 2, etc., accordingly OXd0, OXd1, OXd2, etc.) on the abdomen, and then painted on the flanks with FITC. Twenty-four hours after FITC painting, the accumulation of FITC-labeled DCs was evaluated in ILNs. In non-OX-treated mice, 1.3% of the cells in ILNs were FITC+CD11c+ DCs. A comparable proportion was obtained in ILNs of vehicle-only (acetone)-treated mice (Fig. 5A). A lower proportion of FITC+CD11c+ cells was obtained from ILNs of OXd0 mice, with an even greater decrease in OXd1 ILNs, reaching a nadir in OXd2 and OXd3 ILNs (0.2%). Recovery to the levels found in nodes of nonimmunized mice was apparent in OXd5, and the restored/enhanced accumulation of newly activated DCs continued to OXd14 (Fig. 5A). Thus, the accumulation of newly activated APCs in sensitized LNs was transiently reduced between days 2 and 5 after OX, even though the total number of CD11c+ cells was actually increased after immunization (Fig. 5A and inset). Fig. 5A shows representative results (n 3). The decrease in access of afferent lymph to the immunized LNs and/or the increase of efferent lymph output could explain the reduction of Evans blue accumulation in OXd4 ILNs. However, because APCs are rare in efferent lymph (9), the reduction of FITC+CD11c+ cell accumulation in OXd4 ILNs is likely due to impaired afferent LV access to the LN.
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Because the accumulation of newly activated APCs was transiently reduced in draining LNs, we asked whether T cells could be primed to another Ag during this period. Nontreated, OXd2, OXd4, and OXd7 mice were immunized with OVA in CFA in both flanks and in the tail. Seven days later, PLNs were removed and evaluated for T cell recall proliferation to OVA. OVA-specific proliferation was reduced when cells were obtained from OVA-OXd2 mice, compared with those from OVA-only immunized control mice. The proliferative response of cells from OVA-OXd4 mice approached that of non-OX-treated mice and was actually over compensated in OVA-OXd7 cells (Fig. 5B). This overcompensation which is apparent in LNs from mice treated with OX, immunized with OVA 7 days later and then analyzed after an additional 7 days is likely due in part to the recovery of DC arrival and is probably also influenced by the remodeled HEVs and lymphatic vessels (see below). These results indicate that inhibition of T cell priming to a second Ag occurs after immunization, is transient, and correlates in time with the effects on LV vessel function.
Lymphangiogenesis in the skin after immunization is associated with recovery of afferent lymphatic vessel function
Because afferent LV function was impaired after immunization, we next investigated whether the vessels were disrupted at the skin-painting site. We quantified LV number by counting the number of CD31+ or LYVE-1+ vessels under fluorescent microscopy per entire x40 field. Twenty-five random fields were counted in skin sections in the epidermal and dermal area, excluding s.c. tissue (20 random skin sections per mouse, two mice per group). The number of CD31+ vessels increased, reaching a peak at OXd4 (p < 0.001), and was maintained at OXd7, indicating angiogenesis. The number of LYVE-1+ vessels was comparable in OXd4 skin and nontreated skin but almost doubled in OXd7 skin (p < 0.001), indicating that lymphangiogenesis in the skin occurred and peaked at day 7 after immunization (Fig. 6A).
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R and B cells mediate HEV and LV cross-talk after immunizationBecause afferent LV biological function is transiently impaired at day 4 after immunization and then recovers and correlates with the recovery of the mature HEV phenotype, we next investigated whether there was an effect on LVs in the draining LNs. First, we evaluated LV and HEV morphology in LNs by LYVE-1 and PNAd double staining at various times after immunization. Lymphangiogenesis in the LN was apparent as early as day 4, even before lymphangiogenesis occurred in the skin, and peaked at day 7 after OX immunization. Dramatically, HEVs were located in the area surrounded by the LV network (Fig. 6B, b and c). Several LVs appeared to interact directly with HEVs (Fig. 6B, d–f, arrowhead), suggesting the possibility of LV-HEV cross-talk. Specifically, in OXd4 LNs, vessels appearing to express both PNAd and LYVE-1 were frequently observed, especially in those individual LVs that were some distance away from the major LV network, suggesting a very close interaction between these two vessels (Fig. 6Be). This observation provides another mechanism for the recruitment of immature blood-borne DCs via HEV in the immunized LN (30), indicating that HEV plasticity after immunization has important functional consequences. These double-positive vessels were rare in control and OXd7 LNs (n = 3 mice per group).
Because HEV gene recovery was contingent on B cells, we then asked whether B cells also contribute to the lymphangiogenesis in the draining LNs. No LN lymphangiogenesis occurred in OX day 4 or 7 µMT LNs (Fig. 6C, a–c), indicating that B cells are important in triggering lymphangiogenesis in the early time after immunization, confirming a previous observation (31). However, at day 14 after immunization, lymphangiogenesis occurred even in the absence of B cells, indicating that factors in addition to those derived from B cells contribute to this process (Fig. 6Cd). Furthermore, during the extended period before lymphangiogenesis occurred in the LNs of µMT mice, the effects on HEV gene expression regulation were exaggerated; PNAd expression was extremely low and MAdCAM-1 expression was dramatically increased, compared with WT mice (Fig. 6C, e–h, compare with Fig. 1A, i–l). By day 14, lymphangiogenesis occurred, PNAd recovered, and MAdCAM-1 was reduced to its normal low level (Fig. 6C, e–h). The correlation between lymphangiogenesis and HEV phenotype regulation after immunization in µMT mice emphasizes the synchrony between LV and HEV.
We previously found that LTR is expressed on PNAd-positive HEVs (27). In this study, we extended these findings and also found coexpression of LYVE-1 and LTR on HEVs from LNs of unimmunized mice (Fig. 6D, a and b). Thus, both HEVs and LVs express the LTR.
We next asked whether LV and HEV cross-talk is mediated by LTR. Mice were injected i.p. with 100 µg/mouse of either LTR-Ig or control-Ig 24 h before OX immunization. PLNs were analyzed 4 days after OX (OXd4). PNAd+LYVE-1+ vessels are yellow in the merged image (arrowhead) (Fig. 6Ea). Pretreatment with LTR-Ig did not significantly affect the number of vessels that stained individually with MECA-79 or anti-LYVE-1 but did drastically reduce the number of vessels positive for both markers (p < 0.001) (Fig. 6Eb), indicating that signaling through the LTR mediates LV and HEV cross-talk.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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R is crucial for regulation of HEV gene expression. We extend these observations to situations of immunization, demonstrate the significant role of B cells in recovery of HEV maturity after immunization, and, importantly, reveal that control of the LTR itself, is a crucial aspect of HEV regulation. Next, we show the plasticity of LVs with loss of function and subsequent lymphangiogenesis in the course of immunization, with remodeling in both the skin and the LN. The LTR is again implicated in its expression on LVs, and B cells are seen as important, but not the sole, players in the remodeling of LVs. There is a temporal concordance in the function of LVs and the maturity of HEVs. The concordance in time, function, LTR expression, the importance of B cells, and the close physical contact between LVs and HEVs in the remodeling process provide insight into the nature of the cross-talk and synchrony of HEVs and LVs.
Remodeling after immunization reveals HEV plasticity and involves the LTR
The plasticity of HEVs during an immune response is evidenced by the HEV remodeling process, which involves down-regulation of mature HEV genes on existing HEVs, and, most likely, generation of new HEVs. This remodeling process is a common phenomenon after immunization with a variety of Ags: contact allergens, OX as reported in this study, and 2, 4-dinitrofluorobenzene (32), SRBC (18, 19), and OVA (Ref. 29 and data not shown). The initiation of HEV remodeling does not require an adaptive immune response, because it occurs in the absence of T and/or B cells (RAG–/–, µMT, and nu/nu mice). Our data provide evidence that the LTR is not only crucial for induction of HEV gene expression and maintenance, but also plays a critical role during the HEV remodeling period. Continuous LTR signaling is required to maintain HEVs; LTR signaling is required for HEV recovery after OX; the LTR itself requires LTR activity to maintain its expression; the LTR is down-regulated at OXd4, which impairs LTR signaling activity on HEVs. Finally, B cells, a likely source of LT12, were found to be essential for HEV recovery. Treatment with LTR-Ig results in down-regulation of MAdCAM-1, whereas immunization initially results in an increase in expression of that adhesion molecule, and then a reversion. This difference suggests that HEV remodeling after OX is more complicated than simply blocking LTR signaling. MAdCAM-1 might respond differently to the transient down-regulation of LTR after OX treatment than to the sustained blockage of LTR signaling following LTR-Ig treatment.
Remodeling of LVs after immunization
Between 2 and 5 days after immunization, the function of afferent LVs, but not LNs or efferent LVs, is severely compromised, as demonstrated by restricted access of Evans blue and FITC+ DCs; a later rebound occurs. The fact that Angeli et al. (31) did not observe the transient insufficiency but did see the later rebound is most likely due to difference in immunization regimens. Several nonexclusive mechanisms most likely contribute to the early functional insufficiency. First, the steady-state function of LVs is probably inadequate to offset the increased interstitial fluid induced by vasodilation at OXd4. The fact that insufficiency also was induced by OVA immunization, a condition that results in less edema, suggests that fluid accumulation is not the sole explanation for these effects. Second, OX immunization might impair the LVs themselves. The strong acute inflammation might disrupt LVs and/or the increased afferent lymph cell content might obstruct LVs. The enlarged LNs also might increase LN internal pressure, causing the collapse of the afferent LVs. A third possibility is that in the course of lymphangiogenesis in the skin, immature LVs might not facilitate APC travel to the LNs. The increased numbers of LYVE-1+ vessels observed in OXd7 skin suggests that lymphangiogenesis occurs at the immunization site. The increased numbers of LVs thereby offset the increased interstitial fluid, and the skin edema subsides by OXd7.
As noted above, LV insufficiency at day 4 after a single immunization is not restricted to OX immunization, because the reduced accumulation of Evans blue also was seen in the draining ILNs of mice immunized with OVA+CFA. Even though there was no obvious edema observed at day 4 after OVA immunization, the dye actually accumulated in the OVA injection site and not in the LN. Therefore, the edema observed in OXd4 skin is the result of LV insufficiency rather than the cause of impaired LV function. Nevertheless, OX might be an extreme situation with regards to LV insufficiency at day 4 after immunization, because immunization with OX (in acetone), presumably toxic, affects a much wider area of skin than does OVA s.c. injection. Lymphangiogenesis also was observed in OVA+CFA injection site at day 7 (data not shown), indicating that immunization-induced lymphangiogenesis in skin is not limited to OX immunization.
Lymphangiogenesis occurs in LNs after immunization and, as in HEV regulation, is influenced by B cells (Ref. 31 and Fig. 6B). The expression of LTR on LVs, as on HEVs, suggests that signaling through that receptor could contribute to these events. The important role of B cells in the early time point after immunization also was indicated by the dramatic influx of B cells in the draining LN. However, lymphangiogenesis occurred in µMT mice in the absence of B cells, albeit at a slower pace, indicating that factors in addition to B cells contribute to lymphangiogenesis. Lymphangiogenesis has been noted in chronic inflammation (33, 34) and even in acute inflammation in which macrophages have been implicated.
LVs and HEVs are tightly coordinated
Mature HEVs are maintained by LTR and factors arriving through afferent LV. Severing afferent LVs prevents the accumulation of afferent lymph factor(s) in LNs; immunization temporarily impairs afferent LV function, resulting in the transient reduction of lymph factor(s) accumulation in the draining LNs. Both procedures result in HEV reversion to an immature phenotype (MAdCAM-1high), suggesting that HEV remodeling is actually the consequence of LV functional inefficiency after immunization.
We propose a model for LV and HEV remodeling after immunization (Fig. 7). After an immunization-priming period (day 0–2), HEVs undergo remodeling. During the remodeling period (day 2–5), angiogenesis in the skin peaks at day 4 and the number of HEVs increases. The efferent LV function remains elevated. B cells trigger early lymphangiogenesis in the LN. In contrast, afferent LVs are functionally insufficient, resulting in accumulation of fluid at the immunization site. Consequently, the accumulation of afferent lymph factors (lymph and cells) is reduced in draining LNs. T cell priming to a second Ag also is inhibited at this time point. HEVs are thus exposed to a reduced amount of newly arrived lymph factors. Meanwhile, LTR expression on HEVs is diminished. HEVs therefore lack continuous stimulator(s) arriving from LVs and reduce their LTR activity and display an immature HEV phenotype (MAdCAM-1highHEC-6STlow), the same gene expression pattern observed after severing afferent LVs. After the remodeling period (days 7–10), lymphangiogenesis peaks at day 7 in both LNs and the immunization site (skin) and LV function recovers. HEVs are surrounded by LV network. LTR also is restored. Sufficient lymph factors arrive in the LN to stimulate HEVs and LTR activity, and the mature phenotype is recovered (Fig. 7).
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R on LVs and HEVs is consistent with the notion that LTR signaling coordinates these two vascular systems, and we suggest that, in addition to B cells, LTR signal activator(s) are delivered by afferent lymph. It is likely that cells in lymph, expressing or capable of inducing the LTR ligands, LT12 or LIGHT, are these LTR signal activator(s). During the steady state, immature DCs continually migrate into LNs through afferent LV and die locally within several days (35, 36, 37). Because immature DCs express LIGHT (38), they could migrate to HEVs to directly activate the LTR signal, although it has been reported that activated DCs do not contact HEVs (39). DCs also express LTR, and signaling through that receptor is important in their homeostasis (40). The activated DCs line up around HEVs to interact with newly homed T cells (41), and thus they might interact with T cells and activate them to increase expression of LT or LIGHT and thus indirectly signal LTR on HEVs. The tight interaction between LVs and HEVs in the remodeling period suggests the intriguing possibility that factors may directly diffuse from LVs to HEVs and influence gene expression. Support for this hypothesis is provided by the observations of Gretz et al. (42) and Sixt and colleagues (43), who noted the presence of tracer in the lumen of HEVs after s.c. injection of labeled dextran. What could be the purpose of the extensive remodeling of HEVs and LVs after immunization? The reduction in Ag access and HEV maturity may serve to prevent an immune response from escalating out of control and may reduce the possibility of an inappropriate response to self Ags in a highly activated environment. We have found that the transient reduction in LN access does have functional consequences when mice are immunized in that period (S. Liao and N. H. Ruddle, manuscript in preparation). In contrast, in the later time after immunization, the generation of new LVs, the maturation of HEVs, and the overcompensated response to a second immunization may set the scene for the generation of memory.
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1 This work was supported by the Anna Fuller Fund (to S.L.) and National Institutes of Health Grants CA16885 and DK57731 (to N.H.R.). 
2 Address correspondence and reprint requests to Dr. Nancy H. Ruddle, P.O. Box 208034, 815 Laboratory of Epidemiology and Public Health, 60 College Street, New Haven, CT 06520-8024. E-mail address: nancy.ruddle{at}yale.edu
3 Abbreviations used in this paper: LN, lymph node; LT, lymphotoxin; PLN, peripheral LN; ILN, inguinal LN; MLN, mesenteric LN; LV, lymphatic vessel; HEV, high endothelial venule; PNAd, peripheral node addressin; OX, oxazolone; SRBC, sheep RBC; WT, wild type.
Received for publication April 14, 2006. Accepted for publication June 15, 2006.
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directs lymphoid organ neogenesis with concomitant expression of peripheral node addressin and a HEV-restricted sulfotransferase. J. Exp. Med. 197: 1153-1163. 
B kinase complex kinase activity controls chemokine and high endothelial venule gene expression in lymph nodes and nasal-associated lymphoid tissue. J. Immunol. 173: 6161-6168. receptor signaling is required for the homeostatic control of HEV differentiation and function. Immunity 23: 539-550. [Medline] receptor requirement for homeostasis of lymphoid tissue dendritic cells. Immunity 22: 439-450. [Medline]This article has been cited by other articles:
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), OXd4 (
), OXd7 (
), OXd14 (
).
