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Correction for Willemsen et al., J Immunol 177 (2) 991-998.
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The Journal of Immunology, 2006, 177: 8875-8876.
Copyright © 2006 by The American Association of Immunologists, Inc.

Corrections

CD8{alpha} coreceptor to improve TCR gene transfer to treat melanoma: down-regulation of tumor-specific production of IL-4, IL-5, and IL-10

R. A. Willemsen, Z. Sebestyén, C. Ronteltap, C. Berrevoets, J. Drexhage and R. Debets

Willemsen, R. A., Z. Sebestyén, C. Ronteltap, C. Berrevoets, J. Drexhage, and R. Debets. 2006. CD8{alpha} coreceptor to improve TCR gene transfer to treat melanoma: down-regulation of tumor-specific production of IL-4, IL-5, and IL-10. J. Immunol. 177: 991–998.

The flow cytometry panels of Figs. 1B and 4B (CD4FITC panels) were mistakenly duplicated. The corrected panels are presented below as dot plots instead of histograms. In the Fig. 1 legend, the penultimate sentence is therefore incorrect and in Materials and Methods, in the fifth sentence under the heading Flow cytometry of gene-modified T lymphocytes, the words "or histograms" should be deleted. The errors do not change the message of either the figures or the paper. The corrected sentence, Fig. 1 and legend, and Fig. 4, are shown below.

The dot plots represent viable T cells gated on forward and sideward light scatter signals.


Figure 1
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  		FIGURE 1. The gp100/A2-specific T cells do not require CD8{alpha} for binding peptide/MHC I ligand. CD8-positive and -negative fractions of primary human T cells were transduced with TCR genes derived from a gp100/A2-specific CTL-296 and, in case of CD8-negative T cells (i.e., CD4+ T cells), also a CD8{alpha}t gene. T cells were subsequently sorted into three populations with the use of PE-labeled gp100/A2 tetramer and FITC-labeled CD8{alpha} mAb: 1) CD8+ T cells expressing the TCR{alpha}beta transgenes; 2) CD4+ T cells expressing the TCR{alpha}beta transgenes; and 3) CD4+ T cells expressing the TCR{alpha}beta and CD8{alpha}t transgenes. These three T cell populations were validated by flow cytometry for the expression of CD4 and CD8 (a) and binding with tetramers (b). See Materials and Methods for details on flow cytometry and sorting of T cells. Dot plot ordinates are set using stainings with a nonrelated HLA-A2-binding tetramer. Data represent one of two donors with similar results.

 

Figure 4
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  		FIGURE 4.
 





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