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CD8 coreceptor to improve TCR gene transfer to treat melanoma: down-regulation of tumor-specific production of IL-4, IL-5, and IL-10

Willemsen, R. A., Z. Sebestyén, C. Ronteltap, C. Berrevoets, J. Drexhage, and R. Debets. 2006. CD8 coreceptor to improve TCR gene transfer to treat melanoma: down-regulation of tumor-specific production of IL-4, IL-5, and IL-10. J. Immunol. 177: 991–998.

The flow cytometry panels of Figs. 1B and 4B (CD4^FITC panels) were mistakenly duplicated. The corrected panels are presented below as dot plots instead of histograms. In the Fig. 1 legend, the penultimate sentence is therefore incorrect and in Materials and Methods, in the fifth sentence under the heading Flow cytometry of gene-modified T lymphocytes, the words "or histograms" should be deleted. The errors do not change the message of either the figures or the paper. The corrected sentence, Fig. 1 and legend, and Fig. 4, are shown below.

The dot plots represent viable T cells gated on forward and sideward light scatter signals.

View larger version (36K): [in this window] [in a new window]   FIGURE 1. The gp100/A2-specific T cells do not require CD8 for binding peptide/MHC I ligand. CD8-positive and -negative fractions of primary human T cells were transduced with TCR genes derived from a gp100/A2-specific CTL-296 and, in case of CD8-negative T cells (i.e., CD4+ T cells), also a CD8t gene. T cells were subsequently sorted into three populations with the use of PE-labeled gp100/A2 tetramer and FITC-labeled CD8 mAb: 1) CD8+ T cells expressing the TCR transgenes; 2) CD4+ T cells expressing the TCR transgenes; and 3) CD4+ T cells expressing the TCR and CD8t transgenes. These three T cell populations were validated by flow cytometry for the expression of CD4 and CD8 (a) and binding with tetramers (b). See Materials and Methods for details on flow cytometry and sorting of T cells. Dot plot ordinates are set using stainings with a nonrelated HLA-A2-binding tetramer. Data represent one of two donors with similar results.

View larger version (24K): [in this window] [in a new window]   FIGURE 4.

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Willemsen, R. A. Articles by Debets, R. Search for Related Content PubMed Articles by Willemsen, R. A. Articles by Debets, R.