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The Journal of Immunology, 2006, 177: 8440-8447.
Copyright © 2006 by The American Association of Immunologists, Inc.

Regulated Expression of Fc{gamma}R in Human Dendritic Cells Controls Cross-Presentation of Antigen-Antibody Complexes1

Yi Liu*, Xiaoni Gao*, Emi Masuda*, Patricia B. Redecha*, Marissa C. Blank* and Luminita Pricop2,*,{dagger},{ddagger}

* Research Division, Hospital for Special Surgery, {dagger} Department of Medicine, Weill Medical College, Cornell University, and {ddagger} Immunology Program, Weill Graduate School of Medical Sciences, New York, NY 10021


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Receptors for IgG (Fc{gamma}R) expressed in dendritic cells (DCs) influence the initiation of Ab-mediated immunity. Dynamic variations in Fc{gamma}R expression allow DCs to adjust their capacity to capture Ab-opsonized Ag. The current paradigm predicts a progressive decline in Fc{gamma}R-mediated phagocytic function upon DC maturation. Surprisingly, we find that expression of the phagocytic receptor Fc{gamma}RIIa is preserved in immature and mature DCs at comparable levels with macrophages. Moreover, phagocytosis of antigenic peptides directed to Fc{gamma}RIIa on DCs leads to dramatic increases in Ag cross-presentation and T cell activation. In immature DCs, high expression of inhibitory Fc{gamma}RIIb correlates with decreased uptake and cross-presentation of Ab-Ag complexes. In contrast, engagement of Fc{gamma}RIIb is not associated with changes in cross-presentation in mature DCs. We provide evidence that Fc{gamma}RIIb expression is patently reduced in mature DCs, an effect that is modulated by treatment with cytokines. The regulated expression of activating and inhibitory Fc{gamma}Rs in DCs emerges as a critical checkpoint in the process of Ag uptake and cross-presentation


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 Disclosures
 References
 
Dendritic cells (DCs)3 are initiators of Ag-specific immune responses and key regulators of T cell activation. DCs promote infectious and tumor immunity, while setting a threshold for activation of autoreactive T lymphocytes. Specific checkpoints in the maturation and activation state of DCs orchestrate the outcome of a given immune response.

Fc{gamma}R are important triggers of DC activation. Signaling through Fc{gamma}R requires Abs as ligands, revealing the main function of these receptors as mediators of adaptive immune responses. Human DCs express several types of Fc{gamma}R (1, 2, 3). Fc{gamma}RI expressed on DCs consist of the IgG-binding {alpha}-chain and the ITAM-bearing {gamma}-chains. DCs constitutively express Fc{gamma}RIIa and Fc{gamma}RIIb, which share similar extracellular domains, but contain distinct cytoplasmic domains that mediate positive and negative signaling (4, 5). Fc{gamma}RIIa are phagocytic receptors that facilitate internalization of IgG-containing immune complexes. Fc{gamma}RIIb inhibit DC function by virtue of the intracellular ITIM. Blockade of Fc{gamma}RIIb is required for efficient induction of immunity to Ab-coated cells, underscoring the role of Fc{gamma}RIIb as a negative modulator of DC function (4).

In view of the role of Fc{gamma}R as regulators of DC function, we analyzed the kinetics of expression of activating and inhibitory Fc{gamma}R during DC maturation. Fc{gamma}RI, which are highly expressed in monocytes and macrophages (Ma), were down-regulated at early stages of DC differentiation. Low levels of Fc{gamma}RI expression persisted on both immature (iDCs) and mature DCs (mDCs). Fc{gamma}RIIa were expressed at comparable levels throughout DC differentiation, including the mature stages. Fc{gamma}RIIb were found to be highly expressed in iDCs and were down-regulated following maturation of DCs with either TNF-{alpha} or a mixture containing inflammatory cytokines TNF-{alpha}, IL-1beta, IL-6, and PGE2. The expression of Fc{gamma}RIIa and Fc{gamma}RIIb was induced in mDCs by treatment with IL-10 and IL-13, indicating that the expression of Fc{gamma}RII isoforms in these cells can be modulated by cytokine treatment.

DCs acquire exogenous Ag by phagocytosis and cross-present antigenic peptides in association with MHC class I molecules to cytotoxic CD8+ T lymphocytes (6, 7). Interaction of Ab-Ag complexes with Fc{gamma}R influences the ability of DCs to present Ag to T lymphocytes (8, 9). Selective engagement of activating or inhibitory Fc{gamma}Rs has the potential to induce differences in the efficiency of Ag cross-presentation. Targeting influenza antigenic peptides in DCs through activating Fc{gamma}Rs overcame the need for full DC maturation, and induced dramatic increases in IFN-{gamma}-producing influenza-specific CD8+ T cells. Engagement of inhibitory Fc{gamma}RIIb limited phagocytosis and decreased TNF-{alpha} production by DCs, leading to reduced expansion of memory T lymphocytes. The present study characterizes the dynamic regulation of Fc{gamma}Rs during DC maturation and identifies factors that change the balance of Fc{gamma}R in human DCs subsequently affecting the initiation of adaptive immune responses.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 Disclosures
 References
 
Peptides and Abs

The HLA-A*0201-restricted immunodominant influenza A matrix peptide (MP) system was used to stimulate influenza-specific memory CD8+ T cells (10, 11). The 9-mer core MP (GILGFVFTL) and a 17-mer peptide (TKGILGFVFTLTVPSER) containing N- and C-terminal flanking residues were used as antigenic peptides. MP-specific polyclonal antiserum was obtained by immunization of rabbits with 17-mer and showed reactivity with 9-mer and 17-mer MP in immunoblots (see Fig. 3Ad). F(ab')2 of MP-specific rabbit IgG were obtained by pepsin digestion using ImmunoPure F(ab')2 preparation kit (Pierce). Fab of mouse anti-Fc{gamma}RIIa mAb, clone IV.3, and F(ab')2 of anti-Fc{gamma}RI, clone 22.2, and anti-Fc{gamma}RII, clone 7.3 were purchased from Medarex and Research Diagnostics, respectively. Mouse anti-Fc{gamma}RII mAb, clone FLI8.26 was purchased from Research Diagnostics. The rabbit polyclonal Abs reacting specifically with Fc{gamma}RIIa (260) or Fc{gamma}RIIb (Fc{gamma}RIIB/IC) used for Western blotting were previously described (12, 13).


Figure 3
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  		FIGURE 3. Internalization of MP-coated Fc{gamma}R-specific phagocytic probes by DCs. A, Control probes consisting of nonopsonized erythrocytes showed no staining with streptavidin-PE and FITC-labeled rabbit anti-mouse IgG (a). Probes opsonized with rabbit anti-MP polyclonal Abs followed by incubation with titrated amounts of biotinylated MP and stained with streptavidin-PE (b). Probes were opsonized with anti-Fc{gamma}R mAbs and rabbit anti-MP Abs, followed by biotinylated-MP (100 mM), and the binding was verified with anti-mouse IgG-FITC and streptavidin-PE (c). The reactivity of rabbit antiserum generated by immunization with influenza 17-mer peptide was tested in dot blots (d). Preimmune rabbit serum had no reactivity with MP. Serum collected 8 wk postimmunization reacted with MP (10 mM and 1 mM). B, Representative flow cytometry plot showing phagocytosis of PKH26-labeled IV.3- and 7.3-coated probes by iDC (a and b) and mDC (c and d). Internalized MP following uptake of E-MP-IV.3 probes (e) and E-MP-7.3 (f) by mDC was detected in immunofluorescence microscopy with rabbit anti-MP Abs and Alexa 546-conjugated goat anti-rabbit IgG counterstained with IV.3-FITC and 7.3-FITC.

 
Isolation and culture of DCs

Leukopheresed PBMC (~8–10 x 108/sample) were isolated from HLA-A*0201-positive healthy donors by sedimentation over Ficoll-Hypaque (Pharmacia Biotech). T cell-enriched and monocyte-enriched fractions were separated by rosetting with sheep RBC treated with {alpha} 2–3,6,8-neuraminidase (Calbiochem). T cells were frozen in liquid nitrogen and thawed after 6 days and incubated with autologous DC in ELISPOT and Pro5 pentamer-staining assays.

DCs were differentiated from monocyte-enriched fractions in culture by treatment with cytokines (all purchased from R&D Systems unless otherwise specified). iDC were differentiated from monocytes in 6 day cultures in complete medium (RPMI 1640 medium, 1% HEPES, 1% glutamax, and 1% penicillin-streptomycin) supplemented with 1% human plasma, 1000 U/ml GM-CSF, and 10 ng/ml IL-4. mDC were obtained from iDC cultured on day 5 with cytokine mixture consisting of 10 ng/ml TNF-{alpha}, 10 ng/ml IL-1beta, 1000 U/ml IL-6, and 1 µg/ml PGE2 (Sigma-Aldrich) for 24 h. Macrophages were obtained by culturing adherent monocytes (6 x 106cells/well) in 6-well plates with complete medium plus 5% pooled AB human serum PHS (Pel-Freeze Clinical Systems) supplemented with 1000 µg/ml recombinant human GM-CSF. For modulation of Fc{gamma}R expression, iDC and mDC cultures were supplemented with 50 ng/ml IL-13, 25 ng/ml IL-10 (PeproTech), or IL-13 plus IL-10 for 24 h.

Evaluation of receptor expression and Fc{gamma}R-mediated phagocytosis by flow cytometry

For evaluation of Fc{gamma}R expression, DCs were incubated with FITC-labeled anti-Fc{gamma}R mAbs (clones 22.2, IV.3, and 7.3). Membrane expression of CD14, MHC class I (MHC-I), HLA-DR, CD80, CD83, CD86, CD40 was detected with FITC-labeled specific mAbs (BD Pharmingen).

A flow cytometric assay using erythrocytes labeled with the lipophilic dye PKH26 (Sigma-Aldrich) and coupled with biotin/avidin to F(ab')2 of anti-Fc{gamma}R mAb was used to determine the attachment and phagocytosis of specific probes through Fc{gamma}R (14). Briefly, Fab of anti-Fc{gamma}RII clone IV.3, F(ab')2 of anti-Fc{gamma}R mAb (clones 22.2 and 7.3), and F(ab')2 of anti-MP rabbit polyclonal Abs were biotinylated with EZlink-sulfo-NHS-LC-biotin (Sigma-Aldrich). Ox erythrocytes (E) were biotinylated with sulfo-N- hydroxysuccimide-biotin (Sigma-Aldrich) and saturated with streptavidin (Roche Diagnostics). Biotin-streptavidin-coated erythrocytes were opsonized with biotinylated anti-Fc{gamma}R mAb and with biotinylated F(ab')2 of anti-MP rabbit polyclonal Abs, followed by incubation with 100 µM 9-mer or 17-mer MP for 1 h. MP-Fc{gamma}R-specific probes were labeled with PKH26. iDCs and mDCs (5 x 105 cell/ml) were incubated with MP-Fc{gamma}R-specific fluorescent probes (1.25 x 107 erythrocytes/ml) at 37°C for 20 min. For assessment of phagocytosis, noninternalized probes were then lysed and the uptake of PKH26-labeled MP-Fc{gamma}R-specific probes was measured by flow cytometry using a FACScan (BD Immunocytometry Systems) equipped with a standard optical filter set. The PKH26 fluorescence was detected in the FL2 channel and displayed on a logarithmic scale. The phagocytic index (PI) was calculated by multiplying the percentage of DCs containing PKH26+ probes and the mean fluorescence intensity (MFI) of PKH26+ probes/100 cells.

For assessment of attachment, 5 x 105 DCs/ml and 1 x 106 PKH26-labeled erythrocytes/ml coated with biotinylated fragments of IV.3 (E-IV.3) or 7.3 (E-7.3) were centrifuged at 44 x g for 3 min at 4°C, followed by incubation on ice for 5 min (14). The percentage of cells having attached PKH26-labeled probes on the surface was determined by flow cytometry.

Immunofluorescence microscopy

Following phagocytosis of MP-Fc{gamma}R-specific probes, DC (50,000 cells/condition) were cytospun at 800 rpm for 5 min with Cytospin 2 (Shandon) onto Charged Superfrost slides (VWR Scientific). Slides were fixed with cold acetone for 5 min at –20°C and permeabilized with 0.1% saponin for 15 min. Slides were blocked with 2% BSA-PBS for 15 min, incubated with rabbit anti-MP Abs and Alexa-546-conjugated goat anti-rabbit and costained with 7.3-FITC or IV.3-FITC for 1 h at room temperature. Slides were examined with fluorescent microscope (Leica).

Real-time PCR

Total RNA was isolated with the RNeasy kit (Qiagen). cDNA was synthesized from 1 µg of total RNA with random hexamers (Invitrogen Life Technologies). Real-time PCR was conducted with the SYBR Green PCR Supermix (PerkinElmer) and the iQ MultiColor Real-Time PCR Detection System (Bio-Rad), according to manufacturer’s instructions. The PCR consisted of one cycle at 94°C for 3 min, followed by 40 cycles at 94°C for 30 s and 54°C for 30 s. The following primer pairs were used for amplification: Fc{gamma}RIIA forward, 5'-GACTACGGATACCCAAATGTC-3' and Fc{gamma}RIIA reverse, 5'-AAGCCAGCAGCAGCAAAA-3'; Fc{gamma}RIIB2 forward, 5'-GGGATGATTGTGGCTGTG-3' and Fc{gamma}RIIB2 reverse, 5'-ATTAGTGGGATTGGCTGAA-3'; and GAPDH forward, 5'-CAACGGATTTGGTCGTATT-3' and GAPDH reverse, 5'-GATGGCAACAATATCCACTT-3'. During amplification, absorption readings measured the relative amount of amplicon produced in each cycle. This data was used to make a relative determination of gene expression under each experimental condition. All PCR assays were done in triplicate and the data were pooled.

Immunoprecipitation and Western blotting

DC (5 x 106/ml) were solubilized in 1% Nonidet P-40 lysis buffer and immunoprecipitated for 2 h at 4°C with anti-Fc{gamma}RII mAb (FLI8.26) coupled to protein G-Sepharose beads. The immunoprecipitates (20 µg/sample) were separated by SDS-PAGE on 9% polyacrylamide gels and subsequently transferred to nitrocellulose. Blots were incubated for 1 h at room temperature with the polyclonal rabbit anti-Fc{gamma}RIIb (Fc{gamma}RIIb/IC) or anti-Fc{gamma}RIIa (Ab 260) Abs, washed, and incubated with HRP-conjugated donkey anti-rabbit Abs (final dilution 1/3000). The reaction was developed using the ECL Enhanced Western blotting detection kit (Amersham Biosciences).

Production of TNF-{alpha} by DC after incubation with Fc{gamma}R-specific probes

DC (5 x 106/ml) were cocultured in 5 ml of polypropylene tubes with probes opsonized with mAbs IV.3, 7.3, or no Ab, at 1:25 E:T ratios for 18 h at 37°C. Cell-free supernatants were collected and samples were frozen at –70°C. Production of TNF-{alpha} was determined by sandwich ELISA (R&D Systems). OD values were determined by spectrophotometer at 405 nm.

IFN-{gamma} production measured by ELISPOT assay

Following uptake of MP-Fc{gamma}R-specific probes or direct infection with influenza virus, iDC and DC (4 x 104/well) were incubated with autologous T cells (2 x 105/well) for 40–44 h at 37°C in 96-well ELISPOT plates (Millipore) coated overnight with 5 µg/ml of the anti-IFN-{gamma} mAb clone mAb-1-D1K (Mabtech). DCs infected with influenza virus A (PR8/34; Charles River Laboratories; SPAFAS) or pulsed with 100 µM soluble influenza MPs were used as control. Wells without T cells served as negative control. Wells were washed four times with PBS containing 0.05% Tween 20 (Sigma-Aldrich) followed by incubation for 2 h with 1 µg/ml biotin conjugated anti-IFN-{gamma} mAb clone 7B6-1 (Mabtech). Plates were washed four times in PBS with 0.1% Tween 20, stained with the Vectastain Elite kit (Vector Laboratories), and developed with stable diaminobenzidine (Invitrogen Life Technologies). Spots representing the IFN-{gamma}-releasing T cells were counted with a stereomicroscope and reported as spot-forming cells (SFC)/105 cells.

Detection of MP-specific T cells by Pro5 pentamer staining

MP-specific T cells were stained with Pro5 Pentamer (Proimmune), following the manufacturer’s instruction. DC probed with MP-Fc{gamma}R-specific erythrocytes were incubated with autologous T cells for 40–44 h at 37°C as described for the ELISPOT assay. Cells were harvested and resuspended in 50 µl of PBS, incubated with 2 µl of unlabeled MP-specific Pro5 pentamer for 15 min at room temperature, and then stained with 8 µl of Pro5 Fluorotag and FITC-conjugated anti-human CD8 mAb for 20 min. The cells were washed, fixed in 2.5% paraformaldehyde, and analyzed by flow cytometry.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 Disclosures
 References
 
Dynamic variations in Fc{gamma}R expression occur during DC maturation

Down-regulation of phagocytic receptors upon DC maturation supports the view that phagocytic function is abandoned following Ag uptake, while Ag-presenting function is gained (15). Indeed, a decline in Fc{gamma}R expression in DCs was found to be associated with reduced ability to engulf IgG-coated particles (1). Yet, phagocytosis of particulate Ag through Fc{gamma}R is known to occur in DCs and is associated with increased Ag presentation (16, 17).

To address this apparent contradiction, we revisited the expression and function of Fc{gamma}R in DCs. We analyzed the pattern of Fc{gamma}R expression during the process of maturation of monocyte-derived human DCs. Flow cytometric analysis using anti-Fc{gamma}RI mAb (22.2-FITC) indicated that iDC and mDCs have decreased levels of Fc{gamma}RI expression compared with Ma (5.8 ± 0.9 and 5.4 ± 0.9 vs 52.9 ± 2.3) (Fig. 1A). Fc{gamma}RII expression was assessed with two anti-Fc{gamma}RII mAbs (IV.3 and 7.3). mAb IV.3 exhibits high binding to activating Fc{gamma}RIIa and has low interaction with Fc{gamma}RIIb (black histograms, Fig. 1A, inset). In contrast, mAb 7.3 exhibits preferential binding to Fc{gamma}RIIb, while maintaining interaction with Fc{gamma}RIIa (gray histograms, Fig. 1A, inset) (18). Staining with IV.3-FITC resulted in similar MFIs on iDC (51.8 ± 2.9) and mDC (56.7 ± 3.9) suggesting that, Fc{gamma}RIIa expression is sustained at comparable levels at early and advanced stages of DC maturation (Fig. 1A). The staining intensity of IV.3-FITC was moderately higher on Ma (80.4 ± 4.4) than on DCs. The expression of Fc{gamma}RIIb on circulating DCs (4, 5) and cultured DCs (5) assessed with specific anti-Fc{gamma}RIIb mAbs was recently reported. We performed surface staining with 7.3-FITC mAb on DCs and explored potential differences in binding at distinct DC maturation stages. The intensity of staining with 7.3-FITC was higher on iDC compared with mDCs matured with the cytokine mixture (112.8 ± 32.7 vs 51.7 ± 10.6) (Fig. 1A). The reduced binding of 7.3 mAb to mDC was associated with increased expression of HLA-DR, CD86, CD83, and CD40 (Fig. 1B), suggesting that Fc{gamma}RIIb expression was down-regulated concomitantly with the induction of phenotypic markers of DC maturation.


Figure 1
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  		FIGURE 1. Expression of activating and inhibitory Fc{gamma}Rs on human monocyte-derived DCs. A, Surface expression of Fc{gamma}RI (22.2-FITC) and Fc{gamma}RII (IV.3-FITC and 7.3-FITC) on Ma, iDC, and mDC as assessed by flow cytometry. Significant differences in the MFI from 3 to 12 experiments are marked * for p < 0.05 and ** for p < 0.01. Inset, Binding of anti-Fc{gamma}RII mAbs IV.3 (black) and 7.3 (gray) to Fc{gamma}RIIA and Fc{gamma}RIIB stable transfectants. B, Membrane expression of MHC-I, HLA-DR, CD86, CD40, and CD83 on Ma, iDC, and DC was assessed by direct staining with FITC-conjugated mAbs (*, p < 0.05; **, p < 0.01). C, Analysis of Fc{gamma}RIIA and Fc{gamma}RIIB transcripts by RT-PCR and real-time PCR. Left panel, RT-PCR analysis was performed with 1 µg of total cell RNA that was reverse transcribed and 2-fold dilutions of each cDNA were used in the amplification reaction with specific primer pairs for Fc{gamma}RIIA, Fc{gamma}RIIB, and actin (12 ). Right panel, Semiquantitative real-time PCR showing the relative expression and ratio of Fc{gamma}RIIA/Fc{gamma}RIIB transcripts in iDC and mDC compared with Ma differentiated from each donor (n = 10, p < 0.01). D, Fc{gamma}RIIa and Fc{gamma}RIIb protein levels were assessed by Western blotting using whole cell lysates immunoprecipitated with pan-Fc{gamma}RII mAb FLI8.26. Blots were overlaid with rabbit polyclonal Abs specific for the intracellular domain of Fc{gamma}RIIa (260) and Fc{gamma}RIIb (Fc{gamma}RIIB/IC). Lysates obtained from A375 cell line stably transfected with rFc{gamma}RIIA (IIA), Fc{gamma}RIIB2 (IIB2), or vector only (NT) were used for control.

 
We analyzed the expression of Fc{gamma}RIIA and Fc{gamma}RIIB RNA transcripts in human DCs at distinct maturation stages by RT-PCR (Fig. 1C, left panel) (12) and real-time PCR (Fig. 1C, right panel). iDCs generated in culture with GM-CSF and IL-4 predominantly expressed Fc{gamma}RIIB transcripts (Fig. 1C, left and right panel). Fc{gamma}RIIB transcripts were undetectable or low in DCs matured with either TNF-{alpha} alone (DCTNF) or with the mixture of inflammatory cytokines (mDC). Fc{gamma}RIIA transcripts were down-regulated following maturation of DCs with TNF-{alpha} (Fig. 1C, left panel), but persisted in mDCs cultured with inflammatory cytokine mixture (Fig. 1C, right panel). Semiquantitative real-time PCR indicated skewing in the ratio of Fc{gamma}RIIA/Fc{gamma}RIIB in favor of Fc{gamma}RIIB transcripts in iDCs (Fig. 1C, right panel). In contrast, the ratio of Fc{gamma}RIIA/Fc{gamma}RIIB transcripts was increased in mDCs as a result of reduced Fc{gamma}RIIB expression (Fig. 1C, right panel).

To determine whether the differential expression of Fc{gamma}RIIA and Fc{gamma}RIIB transcripts during DC maturation is reflected at the protein level, we analyzed Fc{gamma}RIIa and Fc{gamma}RIIb by Western blotting (Fig. 1D). Lysates obtained from Ma, iDCs, and mDCs were immunoprecipitated with pan-Fc{gamma}RII mAb clone FLI8.26 that binds both Fc{gamma}RIIa and Fc{gamma}RIIb (12, 19). The immunoprecipitated proteins were run on SDS-PAGE followed by immunoblotting with specific Abs raised against the intracellular domain of Fc{gamma}RIIa (260) and Fc{gamma}RIIb (Fc{gamma}RIIB/IC), respectively (18). Lysates obtained from A375 melanoma cells transfected with rFc{gamma}RIIA (IIA), Fc{gamma}RIIB2 (IIB2), or vector only (NT) indicate the specificity of the immunoblotting Abs (Fig. 1D). Fc{gamma}RIIa protein was highly expressed in Ma and was also present in iDCs and mDCs (Fig. 1D). Inhibitory Fc{gamma}RIIb were the predominant Fc{gamma}R isoforms expressed at the immature DC stage. mDCs showed lower expression of Fc{gamma}RIIb protein compared with iDC (Fig. 1D). Collectively, these results reveal dynamic changes in Fc{gamma}R expression that occur in the process of DC maturation.

Phagocytosed Ag is cross-presented with higher efficiency in comparison with soluble Ag (20, 21). It is therefore important to assess the ability of DCs to capture and internalize antigenic particles through phagocytic receptors. Isolated blood DCs have the ability to mediate phagocytosis through Fc{gamma}RI and Fc{gamma}RII (2). We assessed the phagocytic capacity of Fc{gamma}RI and Fc{gamma}RIIa in iDC and mDC generated in culture. There was a 10-fold decrease in Fc{gamma}RI-mediated phagocytosis of E-22.2 probes in iDCs compared with Ma (33.8 ± 3.9 vs 361.7 ± 28.4) that was further reduced in mDC (19.5 ± 2.8) (Fig. 2A). Phagocytosis of E-IV.3 probes was only moderately decreased in iDCs and mDCs compared with Ma (107.3 ± 10.7 and 96.2 ± 13.3 vs 127.0 ± 13.4), indicating that phagocytosis via Fc{gamma}RIIa is preserved during DC maturation. Both iDCs and mDCs showed higher Fc{gamma}RIIa-mediated phagocytosis of E-IV.3 probes compared with phagocytosis of E-22.2 probes mediated through Fc{gamma}RI (Fig. 2A). Interestingly, despite low Fc{gamma}RI expression in DCs, CD86 expression was higher after phagocytosis of E-22.2 probes compared with E-IV.3 probes in both iDCs and mDCs (Fig. 2B). This finding is in agreement with other reports indicating that signaling through the Fc{gamma}RI-associated {gamma}-chain induces a mature phenotype in DCs (6).


Figure 2
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  		FIGURE 2. Phagocytosis and TNF-{alpha} production mediated by activating and inhibitory Fc{gamma}R in human DCs. A, Phagocytosis of PKH26-labeled probes E-22.2, E-IV.3, and E-7.3 was measured by flow cytometry. The PI is presented as mean ± SEM of five to eight experiments (*, p < 0.05 and **, p < 0.01). B, Membrane expression of CD86 on DC following internalization of Fc{gamma}RI- and Fc{gamma}RIIa-specific probes ({square} E-22.2, {blacksquare} E-IV.3). iDCs and mDCs were cultured for 24–48 h following phagocytosis and were stained with FITC-conjugated anti-CD86 mAb. Cells that were not incubated with phagocytic probes were used as control (the MFI was set as 100). Staining with CD86-FITC was expressed as a percentage of control from three individual experiments (*, p = 0.04). C, Assessment of attachment and phagocytosis of PKH26-labeled E-IV.3 and E-7.3 (each coated with 4 ng of mAb) by iDCs from three donors (representative example). D, Modulation of phagocytosis in DCs after cross-linking of Fc{gamma}RIIa and Fc{gamma}RIIb. Left panel, Fc{gamma}RIIa and Fc{gamma}RIIb expression was detected by direct staining with IV.3-FITC and 7.3-FITC. Middle panel, PI of iDC and mDC that have internalized PKH26-labeled E-IV.3 and E-7.3 probes is shown. Right panel, The percent inhibition of phagocytosis following cross-linking of Fc{gamma}RIIb was calculated by setting the PI of E-IV.3 probes as 100. Results are representative of four individual experiments (**, p < 0.001). E, Secretion of TNF-{alpha} by iDC cultured for 18 h E-IV.3 probes and E-7.3 probes as compared with E without Ab was assessed by ELISA (n = 8–10, *, p < 0.02).

 
Cross-linking of activating and inhibitory Fc{gamma}R initiates the inhibitory signaling cascade leading to down-regulation of cellular functions (22). With the identification of Fc{gamma}RIIb expression on DCs (23, 4, 5), we sought to dissect the role of this receptor in phagocytosis. We analyzed attachment and phagocytosis of PKH26-labeled E-IV.3 and E-7.3 by flow cytometry (Fig. 2C). The level of IV.3 and 7.3 mAb on the probes was verified with FITC-conjugated rabbit anti-mouse IgG. In all experiments, the E-7.3 probe was matched to E-IV.3 probe as far as amount of Ab coated onto the probes. At equivalent concentrations of IV.3 and 7.3 mAb used to coat the probe (example using 4 ng mAb/probe), the percentage of iDCs with E-7.3 probes attached to the surface was similar to the percentage of iDCs with attached E-IV.3 probes, while the percentage of iDCs that had internalized E-7.3 probes was lower than the percentage of iDCs that had internalized E-IV.3 probes (Fig. 2C). The internalization of E-7.3 probes was lower than the internalization of E-IV.3 probes in Ma, iDCs, and mDCs (36.8 ± 11.6 vs127.0 ± 13.4, 43.2 ± 15.7 vs107.3 ± 10.7, and 41.3 ± 22.6 vs 96.2 ± 13.3), suggesting that engagement of Fc{gamma}RIIb is associated with decreased phagocytosis (Fig. 2, A, C, and D). In prior studies, we found that cross-linking of surface receptors on monocytes with 7.3 mAbs was associated with phosphorylation of the ITIM motif of Fc{gamma}RIIb, suggesting that 7.3 mAb mediates inhibitory signaling (18). The marked decrease in phagocytosis of E-7.3 probes compared with E-IV.3 probes suggests that phagocytosis of Ab-opsonized particles can be altered by cross-linking of Fc{gamma}RIIa and Fc{gamma}RIIb.

TNF-{alpha} is an essential cytokine required for the maturation of DCs. Maturation of DCs following autocrine production of TNF-{alpha} has been described following viral infection (24). In human and mouse monocytes/Ma, the production of TNF-{alpha} is differentially regulated by activating and inhibitory Fc{gamma}R (18, 25). We investigated the production of TNF-{alpha} by iDCs following phagocytosis of E-IV.3 and E-7.3 probes. TNF-{alpha} production was measured by ELISA in iDC placed in culture for 48 h with E-IV.3, E-7.3, and erythrocytes with no Ab as control (Fig. 2E). There was a marked induction in TNF-{alpha} production by iDCs incubated with IV.3 probes as compared with erythrocytes not coated with Abs (3.2 ± 2.1-fold induction). The production of TNF-{alpha} was significantly lower after incubation of iDCs with E-7.3 probes (1.25 ± 0.9-fold induction, p < 0.02) in comparison with E-IV.3 probes. We did not detect major differences in the membrane expression of MHC-I, HLA-DR, CD80, CD86, CD40, and CD83 following incubation of E-IV.3 probes compared with E-7.3 probes, suggesting that the differences in TNF-{alpha} production were not associated with major phenotypic changes.

We assessed the roles of Fc{gamma}RIIa and Fc{gamma}RIIb in the internalization of antigenic probes coupled with the immunodominant influenza MP and opsonized with anti-Fc{gamma}R mAbs. E-MP-IV.3 and E-MP-7.3 probes were made with biotinylated MP and with fragments of IV.3 or 7.3 mAbs, as described in Materials and Methods (Fig. 3A, a–c). Phagocytosis assessed by flow cytometry revealed reduced internalization of PKH26-labeled E-MP-7.3 probes by iDCs and mDCs compared with PKH26-labeled E-MP-IV.3 probes (Fig. 3B, a–d). Internalization of MP probes was detected by immunofluorescence staining with MP-specific polyclonal rabbit antiserum. Higher numbers of MP-positive particles were detected in immunofluorescence microscopy when MP was delivered by E-MP-IV.3 probes as compared with E-MP-7.3 probes in mDCs (Fig. 3B, e and f), suggesting that Fc{gamma}RIIb acts as a negative regulator of Ag uptake in mature DCs.

Fc{gamma}RIIb deficiency is associated with increased phagocytosis and increased proinflammatory cytokine production, suggesting a role for Fc{gamma}RIIb as a negative regulator of innate immune functions (25, 26). Our results indicate that by establishing molecular constraints that hamper internalization of IgG-opsonized Ag and the autocrine action of TNF-{alpha} in DCs, Fc{gamma}RIIb has the potential to restrain adaptive immune responses.

Cross-presentation of Ab-Ag complexes is dependent on Fc{gamma}R expression on DCs

Peptides derived from phagocytosed Ag are presented by DCs with increased efficiency (20, 27). DCs that acquire Ag by phagocytosis elicit Ag-specific CD8+ T cell immunity (7, 10, 28). Phagocytically active Fc{gamma}R are expressed in blood DCs and targeting Ag to these receptors increases the efficiency of T cell activation (29). As DCs express both activating and inhibitory Fc{gamma}R, their relative expression likely influences the cross-presentation of Ab-complexed Ag (4, 9).

We tested the ability of human DCs from HLA-A*0201 healthy donors to elicit IFN-{gamma} production by influenza-specific memory T cells in a well-characterized antigenic system for Ag presentation and CD8+ T cell activation (11). The HLA-A*0201-restricted 9-aa MP (GILGFVFTL) and a 17-aa peptide (TKGILGFVFTLTVPSER) containing additional N- and C-terminal flanking residues present in the influenza virus sequence were used as antigenic peptides. The 9-mer and 17-mer peptides were coupled to Fc{gamma}R-specific phagocytic probes (as shown in Fig. 3A). Ma, iDC, and mDC from HLA-A*0201 were used to stimulate IFN-{gamma}-production by autologous T cells in the ELISPOT assay. Despite their high ability to phagocytose antigenic probes via Fc{gamma}R, Ma were completely ineffective in cross-presentation (data not shown). iDC and mDC from HLA-A*0201 donors infected with replicating influenza A strain PR/8/34 induced high IFN-{gamma} spot formation and served as positive control (Fig. 4A). Incubation of iDC or mDC with soluble MP peptides elicited relatively low numbers of IFN-{gamma}-producing CD8+ T cells (Fig. 4A). MP peptides directed via Fc{gamma}RI and Fc{gamma}RIIa in iDC induced dramatic increases (20-fold induction for Fc{gamma}RI and 50-fold induction for Fc{gamma}RIIa) in IFN-{gamma}-producing memory T cells as compared with soluble peptides (Fig. 4A). The number of IFN-{gamma}-producing memory T cells was higher when MP was delivered through Fc{gamma}RIIa compared with Fc{gamma}RI in iDC (141.1 ± 15.6 vs 65.1 ± 13.9 SFC/105 T cells). In mDCs, internalization of Ag through activating Fc{gamma}R further amplified cross-presentation and activation of Ag-specific T cells. There was a marked increase (up to 25-fold for 9-mer and up to 7-fold for 17-mer) in IFN-{gamma}-spot-forming T cells by mDCs when the antigenic peptides were coupled to Fc{gamma}RI- and Fc{gamma}RII-specific probes, as compared with soluble MP (355.5 ± 70.0 and 400.3 ± 97.1 vs 55.7 ± 8.1 and 54.3 ± 4.3 SFC/105 T cells, p < 0.05). The cross-presentation of MP probes directed through either Fc{gamma}RI or Fc{gamma}RIIa in mDCs was similar (Fig. 4A). These results point out the novel observation that cross-presentation by mDCs can be enhanced when Ag is given by the route of activating Fc{gamma}R.


Figure 4
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  		FIGURE 4. Differential expression of Fc{gamma}R on DC alters cross-presentation of influenza MP to memory T cells. A, IFN-{gamma}-producing autologous influenza specific memory T cells were evaluated by ELISPOT assay. iDCs and mDCs from HLA-A*0201-positive donors were incubated with 100 µM soluble 9-mer (MP) or infected with replicating influenza A strain PR/8/34 (Flu) (left panel). iDCs and mDCs from HLA-A*0201-positive donors were probed with 100 µM 9-mer or 17-mer coupled to Fc{gamma}RI (E-MP-22.2) and Fc{gamma}RIIa (E-MP-IV.3) phagocytic probes (middle and right panels). DCs were subsequently used to stimulate autologous T cells at a 1:5 ratio. B, ELISPOT assay for IFN-{gamma}-producing influenza memory T cells cultured with iDCs (left panel) and mDCs (right panel) following uptake of 9-mer and 17-mer coupled to IV.3 probes (E-MP-IV.3) and 7.3 probes (E-MP-7.3). Significant differences are marked *, p < 0.05. C, Detection of MP-specific CD8+ T cells by Pro5 pentamer staining in flow cytometry. T cells isolated from three independent donors were cultured with autologous iDCs and mDCs probed with E-MP-IV.3 and E-MP-7.3, and control probes with no Ab. The percentage of MP-specific pentamer binding CD8+ cells from one representative experiment is shown.

 
To determine the role of inhibitory Fc{gamma}RIIb in cross-presentation, we quantified IFN-{gamma}-producing T cells stimulated with DCs incubated with E-MP-7.3 probes. Cross-linking of Fc{gamma}RIIb in iDC was associated with a 50% reduction in the number of IFN-{gamma}-producing T cells compared with E-MP-IV.3 probes (Fig. 4B, left panel). Interestingly, no decrease in cross-presentation was detected following cross-linking of Fc{gamma}RIIb by E-MP-7.3 probes in mDC (Fig. 4B, right panel). The percentages of influenza-specific memory CD8+ T cells after incubation with DCs loaded with E-MP-IV.3 probes and E-MP-7.3 probes evaluated by MP-specific HLA-A*0201 pentamer binding were analogous with the results obtained in ELISPOT (Fig. 4C). The delivery of E-MP-IV.3 probes compared with soluble MP into iDCs and mDCs determined clonal expansion illustrated by a marked increase in the percentage of Pro5 MHC pentamer-binding CD8+ T cells (Fig. 4C). The percentage of double-positive CD8+ and Pro5 MHC pentamer-binding T cells was reduced by 34.7 ± 0.42%, p < 0.01 when Fc{gamma}RIIb was cross-linked with E-MP-7.3 probes compared with E-MP-IV.3 in iDCs (Fig. 4C). In mDCs, phagocytosis of E-MP-7.3 probes was associated with reduced expansion of MP-specific CD8+ cells by 15.8 ± 2.1%, p < 0.01 as compared with E-MP-IV.3 probes (Fig. 4C).

The regulation of Fc{gamma}R expression by cytokines enables the adaptation of Fc{gamma}R-bearing cells to changes in the microenvironment. Induction of Fc{gamma}RI by IFN-{gamma} in iDCs leading to augmented uptake of Ab-opsonized antigenic particles has been documented (30). Likewise, inhibitory Fc{gamma}RIIb are subject to regulation by cytokines. Prior work showed that treatment with IL-4/IL-13 and IL-10 leads to the up-regulation of inhibitory Fc{gamma}RIIb in human monocytes (12, 18). Moreover, IL-10 mediates transcriptional activation of the Fc{gamma}RIIB promoter in monocytic cells (18).

We tested whether IL-13 and IL-10 have the ability to modulate the expression of Fc{gamma}RIIa and Fc{gamma}RIIb isoforms in mDCs. The expression of both Fc{gamma}RIIA and Fc{gamma}RIIB RNA transcripts was up-regulated in mDCs by overnight treatment with IL-10, whereas IL-13 did not significantly change the expression of either transcript (Fig. 5A). The combined treatment of IL-13 plus IL-10 significantly increased Fc{gamma}RIIA transcript expression (Fig. 5A). Elevated expression of Fc{gamma}RIIa protein in Western blots using Fc{gamma}RIIa-specific Abs was observed following IL-10 treatment (Fig. 5B). Up-regulation of Fc{gamma}RIIb in mDCs by IL-13 plus IL-10 was detected at protein level in Western blots using Fc{gamma}RIIb-specific Abs (Fig. 5B). The Fc{gamma}RIIa/Fc{gamma}RIIb protein ratio evaluated by densitometry was increased after IL-10 treatment, whereas the combined treatment of IL-13 plus IL-10 decreased the Fc{gamma}RIIa-Fc{gamma}RIIb protein ratio (Fig. 5B). Increased IV.3-FITC binding to mDCs suggested higher Fc{gamma}RIIa surface expression following treatment with IL-10 (Fig. 5C). Increased 7.3-FITC binding to mDCs suggested higher Fc{gamma}RIIb surface expression following treatment with IL-10 and IL-13 plus IL-10 (Fig. 5C). Reduced expression of MHC-I and -II, CD80, and CD86 after treatment of mDCs with IL-10 and IL-13 suggested reversal to a less mature phenotype (Fig. 5D).


Figure 5
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  		FIGURE 5. Cytokine regulation of Fc{gamma}R in mDCs. A, Real-time PCR analysis of Fc{gamma}RIIA and Fc{gamma}RIIB RNA transcripts in mDCs cultured for 24 h in complete medium, or in medium supplemented with IL-10 (25 ng/ml), IL-13 (50 ng/ml), or a combination of IL-10 plus IL13 (n = 4–5, *, p < 0.05). B, Fc{gamma}RIIa and Fc{gamma}RIIb protein levels in mDCs cultured with medium, IL-10, IL-13, and IL-10 plus IL-13 assessed in Western blotting. Cell lysates were immunoprecipitated with pan-Fc{gamma}RII mAb FLI8.26 and blotted with rabbit polyclonal Abs specific for the intracellular domain of Fc{gamma}RIIa (260) and Fc{gamma}RIIb (Fc{gamma}RIIB/IC) (upper panels). Cytokine-induced changes in the Fc{gamma}RIIa/Fc{gamma}RIIb protein ratio were determined by densitometry (lower panel). C, Surface binding of IV.3-FITC and 7.3-FITC was assessed by flow cytometry following mDC treatment with medium, IL-10, IL-13, and IL-10 plus IL-13 (n = 5–7, *, p < 0.05). D, Expression of maturation markers and costimulatory molecules on mDC after treatment with IL-10 and IL-13. Membrane expression of MHC-I, HLA-DR, CD80, CD40, and CD86 on mDCs cultured for 24 h in complete medium, medium supplemented with IL-10 (50 ng/ml), IL-13 (25 ng/ml), or a combination of IL-10 plus IL13. Results from five to seven individual experiments are shown.

 
We investigated whether the phagocytosis of E-IV.3 and E-7.3 probes by mDCs was altered by the treatment with IL-10 and IL-13. The phagocytosis of E-IV.3 probes was increased in mDCs treated with IL-10 (167.7 ± 26.7%, p = 0.05) compared with control mDCs (Fig. 6A). IL-13-treated mDCs had similar phagocytosis of E-IV.3 probes (113.1% ± 4.1) with control mDCs (Fig. 6A). Treatment of mDCs with IL-13 plus IL-10 increased phagocytosis of E-IV.3 probes compared with control mDCs (160.9 ± 25.4%, p = 0.06) (Fig. 6A). We assessed whether the uptake of E-7.3 vs E-IV.3 was altered by the treatment of mDCs with IL-10 and IL-13 (Fig. 6B). Decreased phagocytosis of E-7.3 probes compared with E-IV.3 probes was observed in all conditions. The percent decline in phagocytosis of E-7.3 vs E-IV.3 probes was not significantly different in mDCs treated with medium (72.3 ± 12.7%), IL-10 (64.1 ± 15.2%), IL-13 (67.9 ± 18.1%), or IL-13 plus IL-10 (62.9 ± 14.8%) (Fig. 6B). These results suggest an important role for cytokines in regulating the expression of activating and inhibitory Fc{gamma}R in DCs.


Figure 6
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  		FIGURE 6. Cytokine regulation of Fc{gamma}RII-mediated phagocytosis in mDCs. A, Phagocytosis of E-IV.3 probes by mDCs cultured in complete medium, IL-10 (25 ng/ml), IL-13 (50 ng/ml), or IL-10 plus IL-13. Results are expressed as percentage change in PI compared with control, set as 100 (n = 3, *, p < 0.05). B, Percent inhibition in the PI of E-7.3 probes compared with E-IV.3 probes measured in mDCs cultured in complete medium, IL-10, IL-13, and IL-13 plus IL-10 (n = 3).

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
Disclosures
 References
 
With this study, we show that differential expression of activating and inhibitory Fc{gamma}R during DC maturation critically influences the cross-presentation of Ag-Ab complexes. Our findings corroborate the requirement for activating Fc{gamma}RI and Fc{gamma}RIIa for efficient induction of CD8+ T cell immunity and verify the role of inhibitory Fc{gamma}RIIb as negative regulators of cross-presentation. Alterations in Fc{gamma}R expression affect DC function primarily at the level of Ag uptake. The rapid and marked decline in Fc{gamma}RI expression explains the overall reduction in Fc{gamma}R-mediated phagocytic function associated with DC maturation. In contrast to Fc{gamma}RI, we find that expression of Fc{gamma}RIIa is preserved at immature and mature stages of DC differentiation, as is the ability to effectively capture Ag via Fc{gamma}RIIa-mediated phagocytosis. Unexpectedly for mature DC, acquisition of Ag by Fc{gamma}RI- and Fc{gamma}RIIa-mediated phagocytosis had far superior ability to activate Ag-specific CD8+ T cells. This property may enable mature DCs to boost T cell responses against IgG-opsonized Ag in tissues and at sites of immune complex deposition.

Recent reports have revealed a role for Fc{gamma}RIIa in Ag presentation mediated by plasmacytoid DCs (31, 32). Ag-specific T cell responses were detected only in patients who mounted a humoral response following immunization, pointing out the critical role of Ag-specific Abs in the uptake and presentation of exogenous Ag in vivo (32). Moreover, Fc{gamma}RIIa on plasmacytoid DCs is believed to enable the uptake of DNA-containing Ab complexes present in the serum of systemic lupus erythematosus patients and the induction of Ag-specific T cell responses (31). Our results suggest an essential function for Fc{gamma}RIIa expressed on monocyte-derived DCs in the cross-presentation of Ab-opsonized exogenous Ag to CD8+ T cells. These findings underscore the role of Fc{gamma}RIIa on DCs in the regulation of adaptive immune responses driven by self or foreign Ag-specific T cells.

Fc{gamma}RIIb receptors were recently defined as important negative regulators of DC activation and function (4, 5). Interestingly, we detected high expression of Fc{gamma}RIIb in iDC, whereas in mDCs Fc{gamma}RIIb were markedly down-regulated. Differences in expression levels of Fc{gamma}RIIb at various stages of DC maturation point out the versatile nature of Ab-Ag complex interactions with DCs. The efficient delivery of Ag coupled to Fc{gamma}RII-specific Abs is expected to enhance naive and recall responses by T cells. Our study shows that, when Fc{gamma}RIIb is expressed, its engagement by Ab-Ag complexes can result in inhibition of DC activation and Ag uptake. Various targeting Abs directed against the extracellular domain of Fc{gamma}RII display differential binding to Fc{gamma}RIIa and Fc{gamma}RIIb, thus influencing the functional outcome (4, 5). As targeting Ags to phagocytic receptors on DCs is regarded as a promising approach to modulate immunity or tolerance, our findings shed new light on the function of Fc{gamma}R in DC function.

In search of factors that modify Fc{gamma}R expression, we found that IL-10 modulated the expression of Fc{gamma}RIIa and Fc{gamma}RIIb in DCs. Our results suggest that IL-10 increased the ratio of Fc{gamma}RIIa vs Fc{gamma}RIIb on mDCs, an effect that could lead to enhanced uptake of Ag-Ab complexes. IL-10 is regarded as an immunoregulatory cytokine that limits autoimmune reactions (33, 34). Unexpectedly, patients with rheumatoid arthritis showed enhanced responsiveness to immune complex stimulation following treatment with IL-10 (35), an effect that could be explained by increased Fc{gamma}RIIa-mediated uptake of immune complexes. In autoimmune diseases associated with elevated IL-10 serum levels, such as systemic lupus erythematosus, increased uptake of DNA-Ab complexes through Fc{gamma}RIIa by DCs could stimulate autoreactive T cells and lead to amplification of the autoimmune reaction (31).

Genetic differences in the binding affinity of IgG subclasses by DCs from donors bearing the Fc{gamma}RIIa H/H131 and Fc{gamma}RIIaR/R131 variants (36, 37, 38) associate with changes in the activation and function of DCs (5). Genetic alterations in the promoter of the human FCGR2B gene that correlate with altered gene expression (39, 40) may influence the activation state of DCs and their responsiveness to immune complex stimulation. Given that Fc{gamma}R with opposing function are often coexpressed on DCs, selective targeting of Ag to activating or inhibitory Fc{gamma}R is a complex task. Further dissection of acquired and genetic factors involved in the regulation of Fc{gamma}R will add to our understanding of Ab-mediated Ag uptake in human DCs and will advance current strategies to reprogram the activation of T cells.


   Acknowledgments
 
We thank Catherine Sautes-Fridman (Laboratoire d’Immunologie Cellulaire et Clinique, Institut National de la Santé et de la Recherche Médicale, Unité 255, Paris, France) for providing the anti-Fc{gamma}RIIa- and Fc{gamma}RIIb-specific Abs, and Matthew Albert for help at the start of the project.


   Disclosures
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
References
 
The authors have no financial conflict of interest.


   Footnotes
 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 This work was supported by grants from National Institutes of Health (NIH)/National Institute of Arthritis and Musculoskeletal and Skin Diseases (RO1 AR049765 and R21 AR050643), the Lupus Research Institute, and the Arthritis Foundation (to L.P.). This investigation was conducted in a facility constructed with support from Research Facilities Improvement Program Grant Number C06-RR12538-01 from the National Center for Research Resources, NIH. Back

2 Address correspondence and reprint requests to Dr. Luminita Pricop, Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021. E-mail address: PricopL{at}hss.edu Back

3 Abbreviations used in this paper: DC, dendritic cell; Ma, macrophage; iDC, immature DC; mDC, mature DC; MP, matrix peptide; MHC-I, MHC class I; PI, phagocytic index; MFI, mean fluorescence intensity; SFC, spot-forming cell. Back

Received for publication February 8, 2006. Accepted for publication October 2, 2006.


   References
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 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 Disclosures
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