Files in this Data Supplement:

• Movie 1 – (QuickTime Movie, 1,608 KB) - 3-D reconstruction of normal human T cells using confocal microscopy showing the localization of CG-NAP/AKAP450 (green) at the centrosome with trailing extensions along the microtubules (red) in locomotory T cells. The yellow hue shows the area of colocalization.
  
• Movie 2 – (QuickTime Movie, 1,671 KB) - 3-D image of a polarized normal human T cell crosslinked via LFA-1 and stained with CG-NAP/AKAP450 and LFA-1. The image was rotated in various directions to show the precise colocalization of LFA-1 (green) and CG-NAP/AKAP450 (red) at the centrosome. Overlay of CGNAP/AKAP450 and LFA-1 can be seen at the base of the trailing cell extensions.
  
• Movie 3 – (QuickTime Movie, 2,971 KB) - Fibronectin and immunostained for CG-NAP/AKAP450 (red) and LFA-1 (green). CG-NAP/AKAP450 is detected as a compact spot with little or no association with LFA-1, in contrast with the cells migrating on ICAM-1 (Movie 2).
  
• Movie 4 – (QuickTime Movie, 1,058 KB) - 3-D image of HUT-78 human T lymphoma cell crosslinked via LFA-1 and transfected with the C-terminal GFP/CG-NAP/AKAP450 protein. Overexpression of C-terminal GFP/CG-NAP/AKAP450 protein visualized at the centrioles led to cessation of locomotion, cells remained adherent but did not undergo significant net body translocation over more than 60 minutes observation.
  
• Movie 5 – (QuickTime Movie, 1,233 KB) - 4- D Confocal microscopy of HUT-78 human T lymphoma cells triggered via LFA-1 and transfected with an empty EGFP vector. Cells retained their motile phenotype in comparison to the cells transfected with the C-terminal GFP/CG-NAP/AKAP450 protein (Movie 4). 45 Z-stacks were sequentially acquired over 15 min interval.