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* Department of Immunology, Duke University Medical Center, Durham, NC 27710;
Blood Research Institute, Blood Center of Southeastern Wisconsin, and
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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1 (4, 7, 8). Published studies have clearly demonstrated that LAT is essential for T cell activation, thymocyte development, and homeostasis (9, 10, 11, 12). Despite the importance of LAT in T cells, LAT is only present in T cells, NK cells, mast cells, and platelets (4, 13). Recently, LAT was also found in pre-B cells (14). We along with others attempted to identify whether LAT-like molecules exist in B cells and other cell types; two LAT-like molecules, non-T cell activation linker (NTAL)/linker for activation of B cells (LAB) and LAX, were identified (15, 16, 17). Non-T cell activation linker/LAB, which is expressed in B cells, NK cells, monocytes, and mast cells, is localized to lipid rafts like LAT and is tyrosine phosphorylated upon BCR and FcR stimulation (15, 16). LAX is expressed in T cells, B cells, and monocytes; it has multiple Grb2 motifs very similar to those in LAT. In contrast to LAT and LAB, LAX is not localized to lipid rafts (17). Upon stimulation of Jurkat T cells via the TCR, LAX is phosphorylated and interacts with Grb2, Gads, and the p85 subunit of PI3K. It is also phosphorylated upon BCR engagement. Although LAX contains multiple Grb2-binding motifs, it is not capable of correcting defective signaling pathways in LAT-deficient cells, indicating that LAX functions differently from LAT. Overexpression of LAX in Jurkat cells inhibits TCR-mediated p38 MAPK and NFAT/AP-1 activation. Therefore, in contrast to LAT, LAX probably functions to negatively regulate TCR signaling pathway.
To study LAX function in vivo, we generated LAX-deficient mice by homologous recombination. LAX deficiency had no major impact on lymphocyte development. T and B cells were hyperresponsive upon AgR stimulation. Signaling from the AgRs was enhanced in LAX–/– cells. Thus, our results indicate that LAX functions as a negative regulator.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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The targeting construct used for disruption of the LAX gene is shown in Fig. 1A. After linearization with NotI, this construct was used to transfect embryonic stem (ES) cells. ES transfectants were selected in the presence of G418 and gancyclovir. Of the 230 G418-resistant clones, five clones underwent homologous recombination. Two ES clones were injected into blastocysts of C57BL/6 mice. LAX–/– mice were obtained by interbreeding LAX+/– mice. Southern blot analysis of genomic DNA from ES cells was performed using hybridization solution according to the manufacturer’s protocol (BD Clontech). PCR genotyping was performed using three primers (5'-GACATCGGACTCCAGAGCGATGG-3', 5'-GGTGGCTGAGTCACAGAGTATGG-3', and 5'-TCGCAGCGCATCGCCTTCTA TCG-3').
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Anti-human LAX rabbit sera and mAbs were generated as described previously (17). Polyclonal Abs against mouse LAX were purified from anti-human LAX sera by affinity purification using a GST-LAX (mouse) column. Anti-mouse LAX hybridomas were made by fusion of splenocytes from mice immunized with GST-LAX (mouse) with NSO cells. Anti-phospho-p38, -JNK, and -p44/42 ERK, phospho-Akt (Ser473) and pan-Akt were purchased from Cell Signaling. Other Abs used in this study were anti-phosphotyrosine (4G10) and phospholipase C1 (Upstate Biotechnology), phosphotyrosine (PY20; BD Pharmingen), and 70-kDa heat shock protein (Hsp70; Santa Cruz Biotechnology).
Up-regulation of LAX expression
Jurkat cells (5 x 106 cells) were stimulated for 0, 3, 6, and 15 h in six-well plates coated with anti-CD3
Ab (OKT3 ascites, 1/500 dilution) before lysis. Mouse splenocytes or thymocytes (2 x 107 cells) were stimulated in plates coated with anti-CD3 (2C11; 5 µg/ml), or stimulated with 20 µg/ml anti-mouse IgM F(ab')2 (ICN/Cappel Laboratories) for 0, 3, 6, and 15 h before lysis. Cells were lysed in lysis buffer (1% Brij97, 25 mM Tris-Cl, 150 mM NaCl, and 5 mM EDTA), and postnuclear lysates were immunoprecipitated with anti-LAX Abs. Protein samples were resolved on SDS-PAGE and analyzed by Western blotting with anti-LAX Abs. To test whether up-regulation of the LAX protein requires de novo synthesis, Jurkat cells were cultured in the presence of actinomycin D or cycloheximide at 5 µg/ml starting from 30 min before stimulation. To examine whether the LAX transcript is up-regulated after activation, T and B cells from spleens were stimulated with anti-CD3 or anti-IgM, respectively, for 1 and 3 h. Total RNAs were then extracted from these cells and used in RT-PCR with LAX-specific primers (5'-TTCATCAGATGTTTCGGTGGCTA-3' and 5'-TCAAATGATTATGTCAATATCCCCACAG-3') and actin-specific primers (5'-ACTCCTATGTGGGTGACGAG-3' and 5'-CAGGTCCAGACGCAGGATGGC-3').
Flow cytometry
Single cell suspensions were prepared from mouse thymus, spleen, lymph node, and bone marrow and were stained with the following Abs: FITC-conjugated anti-CD4, CD21, IgM, and IgD; PE-conjugated anti-IgM, CD23, Fas, and Fas ligand; and allophycocyanin-conjugated anti-CD8, B220, and IgM (BD Pharmingen). For detection of apoptosis, activated CD4+ T cells or B cells were stained with annexin V/7-aminoactinomycin using the apoptosis detection kit from BD Pharmingen. Flow cytometry was performed and analyzed using CellQuest software.
For CFSE labeling, T and B cells were purified from spleens by MACS (Miltenyi Biotec) and incubated with 5 µM CFSE in 5% FBS in PBS for 5 min at 37°C. Labeled cells were then stimulated with anti-CD3 and anti-CD28 for T cells or anti-mouse IgM F(ab')2 for B cells, or with PMA (40 ng/ml) and ionomycin (500 ng/ml) for 2 days before FACS analysis.
Serum Ig level, immunization, and immunohistochemistry
Basal levels of different Ig isotypes in naive mice were analyzed by ELISA using anti-mouse IgM, IgG1, IgG2a, IgG2b, IgG3, IgA, and IgE Abs (Southern Biotechnology). For T-dependent Ab response, 5- to 8-wk-old mice were immunized i.p. with 50 µg of alum-precipitated nitrophenylacetyl chicken -globulin (NP-CGG) and challenged with the same dose of NP-CGG on day 21. Sera on days 0, 7,14, 21, 26, and 31 after initial immunization were collected. NP-specific IgM and IgG titers were determined by ELISA on 96-well plates coated with NP-BSA.
Frozen sections (5 µm thick) from spleens of 5- to 8-wk old mice were sliced and mounted on poly-L-lysine-coated glass microscope slides. The sections were fixed with cold acetone and stained with GL-7-biotin and B220 after blocking with BSA. These slides were then incubated with the secondary Abs, goat 
-rat Ig-HRP and streptavidin-alkaline phosphatase, for 1 h and developed with substrates for these two enzymes (blue indicates GL-7, and red identifies B220).
MAPK activation, cell proliferation, and IL-2 production
CD4+ T cells were purified from splenocytes by positive selection using MACS beads. B cells were purified from spleens by negative selection (depleting CD4+, CD8+, Mac1+, and Gr1+ cells). Purified T cells were stimulated with anti-CD3 and -CD28 (each at 10 µg/ml) for 0, 2, 5, 10, 30, and 60 min before lysis. Purified B cells were stimulated with anti-mouse IgM F(ab')2 (20 µg/ml) for 0, 2, 5, 10, 30, and 60 min before lysis. Protein samples were separated by SDS-PAGE and transferred to nitrocellulose. After incubation with primary Abs, nitrocellulose membranes were washed three times and probed with either anti-mouse or rabbit Ig-conjugated to Alexa Fluor 680 (Molecular Probes) or IRDye800 (Rockland). Membranes were then visualized with the Li-Cor Bioscience Odyssey system.
For proliferation, purified CD4+ T cells (4 x 105 cells/well in a 96-well plate) were incubated in medium alone, anti-CD3 (0.5 or 5 µg/ml) and anti-CD28 (1 µg/ml), or PMA (40 ng/ml) plus ionomycin (500 ng/ml) for 48 h. Purified B cells were incubated in medium alone, anti-mouse IgM F(ab')2 (10, 20, and 40 µg/ml), or PMA (40 ng/ml) plus ionomycin (500 ng/ml) for 48 h. Cells were stimulated in triplicate, followed by a 6- to 8-h pulse with 1 µCi/well [3H]thymidine. Incorporation of [3H]thymidine was measured using a liquid scintillation luminescence counter (PerkinElmer). For IL-2 production, CD4+ T cells (4 x 105 cells/well in a 96-well plate) were incubated in medium alone, anti-CD3 (5 µg/ml) and anti-CD28 (1 µg/ml), or PMA (40 ng/ml) plus ionomycin (500 ng/ml) for 24 h. IL-2 in the culture supernatant was assayed in triplicate by ELISA using an OptEIA mouse IL-2 kit (eBiosciences).
Ca2+ flux
Splenocytes from LAX–/– and LAX+/+ mice were first loaded with indo-1 in Ca2+ loading buffer (1x HBSS with 10 mM HEPES and 1% FBS) for 30 min, then stained with PE-CD4 or PE-B220. Flow cytometry was used to measure Ca2+ flux by monitoring the fluorescence emission ratio at 405/495 nm. T cells were stimulated by addition of anti-CD3-biotin (5 µg/ml) and anti-CD4-biotin (1 µg/ml), followed by cross-linking with streptavidin (25 µg/ml). B cells were stimulated with 1 µg/ml anti-IgM F(ab')2.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Our previous results showed that LAX mRNA is expressed predominantly in spleen and thymus. To detect the LAX protein in thymocytes and splenocytes from C57BL/6 mice, we lysed these cells in 1% Brij lysis buffer and immunoprecipitated LAX with anti-LAX mAbs, followed by an anti-LAX Western blot. Surprisingly, LAX protein in these cells was difficult to detect. We speculated that LAX protein might be up-regulated upon T cell activation.
To probe this possibility, we first examined LAX protein expression using Jurkat cells. Jurkat cells were activated with anti-CD3 (OKT3) for 0, 3, 6, and 15 h before immunoprecipitation of LAX. As shown in Fig. 1A, LAX was expressed in unstimulated Jurkat cells as we previously described (17); however, the amounts of LAX protein immunoprecipitated were increased dramatically 6 h after stimulation. Thus, LAX protein is up-regulated upon TCR engagement.
We next determined whether up-regulation of LAX protein requires de novo transcription or protein synthesis. We immunoprecipitated LAX from Jurkat cells activated with OKT3 in the presence of actinomycin D, an inhibitor of transcription, or cycloheximide, an inhibitor of protein synthesis. As shown in Fig. 1B, up-regulation of LAX protein was suppressed by addition of either of these two inhibitors, suggesting that activation of Jurkat cells probably induces transcriptional activation of the LAX gene and synthesis of LAX protein.
We also examined whether LAX is up-regulated upon activation of murine splenocytes and thymocytes. Similar to the results from Jurkat cells, LAX protein in splenocytes or thymocytes was increased after stimulation with anti-CD3 (2C11) for 3 h, whereas LAT remained unchanged (Fig. 1C). We also examined whether LAX protein is up-regulated upon stimulation of splenocytes with anti-IgM F(ab')2. Indeed, cross-linking via the BCR up-regulated LAX protein (Fig. 1D). We determined whether LAX RNA is up-regulated after activation of T and B cells. RNAs were extracted from cells activated for 0, 1, and 3 h; reverse transcribed; and then amplified by PCR using LAX- and actin-specific primers. Amplification of the actin transcript indicated that similar amounts of cDNAs were used. As suggested in Fig. 1E, the LAX RNA level was increased after stimulation. These data indicate that LAX is up-regulated upon AgR stimulation through de novo transcription and protein synthesis.
Generation of LAX-deficient mice
To study the in vivo function of LAX in lymphocyte development and signaling, we disrupted the LAX gene in mouse ES cells by homologous recombination using the standard gene-targeting approach. Bacterial artificial chromosome clones were screened using LAX cDNA. Sequencing of the LAX gene revealed that it contains five exons (Fig. 2A). Exon 2 contains the transmembrane domain. Exon 5 is the largest exon, which contains most of the tyrosine motifs in the cytoplasmic domain of LAX. The targeting vector was constructed by substituting three exons (exons 2, 3, and 4) with a NeoR-containing cassette. After transfection with the targeting construct, ES cells were selected with G418 and screened by PCR. Positive ES clones were analyzed by Southern blot analysis using a probe, as indicated in Fig. 2A. The sizes of the DNA fragments of the targeted allele and WT allele were as predicted (Fig. 2B).
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To confirm that LAX protein is absent in LAX–/– mice, we immunoprecipitated LAX from resting or anti-CD3 stimulated splenocytes. As shown in Fig. 2D, LAX protein was detected in stimulated LAX+/+ splenocytes, but it was absent in LAX–/– splenocytes, indicating that the LAX gene is successfully disrupted in LAX–/– mice.
Lymphocyte development in LAX–/– mice
Examination of LAX–/– mice showed that their thymuses, spleens, and lymph nodes were of normal size. We assessed T cell development by examining CD4 and CD8 expression in the thymus and peripheral lymphoid organs. As shown in Fig. 3A, there were no major alterations in T cell populations from the thymus, spleen, or lymph nodes (not shown). The percentages of CD4+CD8+ or CD4+ and CD8+ cells were similar in LAX–/– and LAX+/+ mice. Analysis of bone marrow cells and splenocytes showed that LAX–/– mice had a normal development of B cells based on the expression of B220, IgM, and CD43 (Fig. 3A and data not shown). Development of peritoneal B1 cells was also normal (data not shown).
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We next examined Ab production in naive and immunized LAX–/– and LAX+/+ mice. Levels of different Ig isotypes in the sera of naive mice were determined by ELISA. Although there were apparent differences in serum IgG3 and IgA concentrations, they were not statistically significant by t test. There were no significant differences in levels of IgM and IgG2b; however, IgG1 and IgG2a levels were higher in LAX–/– mice (
2- to 3-fold). Most noticeably, LAX–/– mice had approximately eight times more IgE than LAX+/+ mice (Fig. 4A).
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Hypersensitive T and B cells in LAX-deficient mice
Our previous data suggest that LAX functions as a negative regulator when it is overexpressed in the Jurkat T cell line. If this is true in vivo, we would expect to see enhanced T cell proliferation upon stimulation via the TCR in the absence of LAX. We first examined CD3-mediated T cell proliferation by [3H]thymidine incorporation. Purified CD4+ T cells from spleens of LAX–/– and LAX+/+ mice were stimulated with anti-CD3 and -CD28 or PMA plus ionomycin. Although thymidine incorporation was similar in T cells from LAX–/– and LAX+/+ mice in response to PMA plus ionomycin (data not shown), it was increased in LAX–/– T cells that were stimulated via CD3 and CD28 (Fig. 5A). We also measured TCR-mediated IL-2 production. T cells from LAX–/– mice produced more IL-2 than those from LAX+/+ mice after activation with anti-CD3 and CD28 Abs (Fig. 5B).
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Enhanced AgR signaling pathways in LAX–/– mice
We examined whether disruption of the LAX gene affects TCR- and BCR-mediated signaling pathways. TCR- or BCR-mediated calcium flux was assayed. As shown in Fig. 7A, calcium flux in LAX–/– CD4+ T cells was increased upon engagement of CD3 and CD4. A similar increase was seen in LAX–/– B cells after cross-linking of IgM (Fig. 7B).
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100, 70, and 38 kDa had an apparent increase in tyrosine phosphorylation. In LAX–/– B cells, proteins with Mr of 70 and 80 kDa had a subtle increase in tyrosine phosphorylation (Fig. 7D). We also examined AgR-mediated MAPK activation by Western blotting with anti-phospho ERK, p38, and JNK Abs. Although there was no difference in JNK activation between LAX–/– and LAX+/+ T and B cells, ERK activation was slightly increased in LAX–/– cells. Moreover, p38 MAPK activation was much stronger and more sustained in LAX–/– T cells (Fig. 7C). BCR-mediated p38 activation was also enhanced in LAX–/– B cells (Fig. 7D). These data correlate with our previous finding that overexpression of LAX in Jurkat cells inhibits TCR-mediated p38 activation.
Because activation-induced cell death was reduced in LAX–/– T and B cells after stimulation via the AgRs, we also examined Akt activation in these cells. Akt is a key molecule that regulates cell survival. As shown in Fig. 7C, T cells from LAX–/– mice showed early and prolonged activation of Akt after anti-CD3 and CD28 stimulation. In LAX–/– B cells, there was increased Akt activation at 5 min after stimulation (Fig. 7D). Enhanced Akt and p38 activation correlated with increased cell survival (reduced cell death) of LAX–/– T and B cells.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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The mechanism by which LAX inhibits T cell activation is not clear. Because TCR- or BCR-mediated signaling events, such as calcium flux, tyrosine phosphorylation, and MAPK activation, were enhanced in the absence of LAX, LAX most likely affects the early stage of T cell activation, for example, tyrosine kinase activation. It is possible that LAX binds other negative regulators, such as phosphatases, to turn off T cell activation; however, we have not been able to detect any interaction between LAX and Src homology region 2 domain-containing protein tyrosine phosphatase 1 or 2 (data not shown). Another possibility is that LAX competes for binding of Grb2, Gads, and p85 with proteins that they normally bind, such as LAT. In the cytoplasmic domain of LAX, there are one p85, two Gads, and five Grb2 binding sites (17). Mutation of these tyrosines abolishes binding of these signaling molecules and the inhibitory function of LAX, indicating that they are important in LAX function. However, the level of LAX protein was low in resting T cells (Fig 1A). Phosphorylation of LAX in activated Jurkat cells was much less than that of LAT (data not shown). Thus, it might be difficult for LAX to compete for LAT binding proteins at least in the initial stage of T cell activation. Perhaps after activation, increased LAX expression could further strengthen negative signaling pathways by binding to a negative regulator or competing with LAT for binding of Grb2, Gads, or p85.
AgR-mediated signaling pathways were enhanced in LAX–/– T and B cells, as evidenced by enhanced Ca2+ flux, ERK, p38, and Akt activation. Interestingly, enhanced AgR signaling did not lead to increased apoptosis. Fas or Fas ligand expression was not altered in LAX–/– cells. Instead, LAX–/– cells survived better than normal cells. Because LAX associates with the p85 subunit of PI3K, we examined whether the PI3K pathway is affected in the absence of LAX. Activation of PI3K activates the serine/threonine kinase, protein kinase B/Akt, which is a key molecule in regulating cell survival (18, 19). Protein kinase B/Akt has been shown to antagonize the proapoptotic functions of BCL2-antagonist of cell death and caspase 9 and positively regulates activation of NF
B and expression of Bcl-xL (20, 21, 22, 23). Interestingly, activation of Akt was enhanced in LAX–/– T and B cells, which is probably the reason for increased survival of these cells upon stimulation. When we examined whether the expression of Bcl-2 or Bcl-xL is increased in LAX–/– cells, we did not detect any significant difference in the expression of these proteins in T or B cells between LAX+/+ and LAX–/– mice. However, it is still possible that there is a difference in the expression of Bcl-2 or Bcl-xL, which might be difficult to detect by Western blotting. Increased cell survival in LAX–/– cells could also be due to enhanced p38 MAPK activation after activation. It has been shown in neutrophils that p38 MAPK can associate with caspase-8 and caspase-3, phosphorylate them, and inhibit their activities (24). This MAPK could play a similar role in lymphocyte survival after engagement of AgRs.
Our present study showed that p38 MAPK activation was prolonged after lymphocyte activation. This result agrees with our previous finding that overexpression of LAX in Jurkat cells inhibits p38 MAPK activation. The p38 MAPK, originally identified for its response to stress stimuli, is activated by ligation of TCR and costimulatory molecules (25, 26). p38 MAP kinase is specifically activated by MAPK kinase 3 (MKK3), MKK4, and MKK6 (27, 28, 29). GADD45 family proteins can physically interact with MKK4 and are important in activation of p38 and JNK. T cells from GADD45–/– mice are severely comprised in their ability to activate p38 and JNK in response to anti-TCR stimulation (30). It is still not clear how p38 activation by MAPK couples to the TCR or costimulatory molecules. How LAX functions in the p38 MAPK pathway remains to be determined in the future.
Although LAX deficiency did not cause significant defects in lymphocyte development, we did observe some abnormalities in LAX–/– mice. Naive LAX–/– mice have spontaneous GC formation. The GC reaction is a characteristic feature of T cell-dependent, humoral immune responses. GC is necessary for the generation of memory B cells and is the site of Ig gene V region hypermutation (31, 32). Formation of GC in naive LAX–/– mice might be due to the presence of T and B cells that are hypersensitive to Ag stimulation in LAX–/– mice.
Unexpectedly, the expression of CD23 in mature LAX–/– B cells was decreased significantly compared with that in LAX+/+ cells. CD23 is the low affinity IgER. This molecule might have several functions, including regulation of T-dependent IgE production and B cell proliferation (33, 34). The immunological roles of CD23 are not clear. In CD23-deficient mice, IgE production is increased (35). These mice have increased inflammation and airway hyperreponsiveness after sensitization and challenging with allergens (36). Interestingly, IgE level in LAX–/– mice was also increased as in CD23–/– mice. It is possible that LAX might also function as an adaptor protein in CD23-mediated signaling pathways and is involved in the regulation of IgE production. The CD23-mediated signaling pathway has not been extensively studied. It should be interesting to determine whether CD23-mediated signaling pathway is normal in LAX–/– mice once we know more about this pathway in the future.
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Acknowledgments |
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Disclosures |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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Footnotes |
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1 This work was supported by National Institutes of Health Grants AI048674 and AI056156. 
2 Address correspondence and reprint requests to Dr. Weiguo Zhang, Department of Immunology, Jones Building, Room 112, Box 3010, Duke University Medical Center, Durham, NC 27710. E-mail address: zhang033{at}mc.duke.edu
3 Abbreviations used in this paper: LAT, linker for activation of T cells; 7AAD, 7-aminoactinomycin D; ES, embryonic stem; GC, germinal center; Hsp70, 70-kDa heat shock protein; MKK3, MAPK kinase 3; NP-CGG, nitrophenylacetyl chicken -globulin; LAB, linker for activation of B cells.
Received for publication September 7, 2004. Accepted for publication February 22, 2005.
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References |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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1 with phosphorylated LAT tyrosine residues: effect of LAT tyrosine mutations on T cell antigen receptor-mediated signaling. J. Biol. Chem. 275:23355.1 and the Ras pathway. Immunity 9:617.[Medline]
B activation, and Bcl-xL levels in vivo. J. Exp. Med. 191:1721. mediates the activation of the p38 and JNK MAP kinase pathways and cytokine production in effector TH1 cells. Immunity 14:583.[Medline]
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) were analyzed by ELISA. The mean value of each isotype is shown at the top of the data group. *, p < 0.05; **, p < 0.001. B, T-dependent Ab response. LAX–/– and LAX+/+ mice were immunized with NP-CGG and challenged on day 21 with the same Ag. Sera were collected on days 0, 7, 14, 21, 26, and 31. NP-specific IgM and IgG titers were determined by ELISA. C, Histological analysis of the spleens from naive and immunized LAX–/– and LAX+/+ mice. GL7+ and B220+ cells are shown as blue and red, respectively.
