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RIIB Regulates Nasal and Oral Tolerance: A Role for Dendritic Cells
* Department of Molecular Cell Biology and Immunology, Vrije Universiteit Medical Center, Faculteit der Geneeskunde, MB Amsterdam, The Netherlands;
Department of Immunology, University Medical Center Utrecht, Utrecht, The Netherlands; and
Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands
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Abstract |
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RIIB failed to develop mucosal tolerance to OVA, and demonstrate in vitro and in vivo a critical role for this receptor in modulating the Ag-presenting capacity of dendritic cells (DC). In vitro it was shown that absence of FcRIIB under tolerogenic conditions led to increased IgG-induced release of inflammatory cytokines such as MCP-1, TNF-
, and IL-6 by bone marrow-derived DC, and increased their expression of costimulatory molecules, resulting in an altered immunogenic T cell response associated with increased IL-2 and IFN- secretion. In vivo we could show enhanced LN-DC activation and increased numbers of Ag-specific IFN--producing T cells when FcRIIB–/– mice were treated with OVA via the nasal mucosa, inferring that DC modulation by FcRIIB directed the phenotype of the T cell response. Adoptive transfer of CD4+ T cells from the spleen of FcRIIB–/– mice to naive acceptor mice demonstrated that OVA-responding T cells failed to differentiate into regulatory T cells, explaining the lack of tolerance in these mice. Our findings demonstrate that signaling via FcRIIB on DC, initiated by local IgG in the mucosa-draining LN, down-regulates DC activation induced by nasally applied Ag, resulting in those defined conditions of Ag presentation that lead to Tr induction and tolerance. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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The mucosa-draining lymph nodes (LN) are essential for the induction of mucosal tolerance, based on the observation that removal of these LN in mice abrogates mucosal tolerance (8). However, nasal tolerance could not be achieved when nose-draining cervical LN (CLN) were replaced by peripheral LN (8), suggesting that neither uptake of Ag through the mucosal surface, nor the uptake and transport of Ag APCs, such as dendritic cells (DC), from the mucosal surface to the draining CLN is sufficient for mucosal tolerance induction. Therefore, we hypothesize that a defined local microenvironment within the specific mucosa-draining LN is essential for the development of mucosal tolerance. As a consequence, specific conditions for Ag presentation by DC may be created in the mucosa-draining LN, resulting in Tr differentiation and tolerance. These conditions are mostly unidentified, but may include the failure or attenuation of DC maturation, as defined by low expression of costimulatory markers and decreased release of cytokines such as IL-12 (12, 13, 14, 15).
Comparison of the gene expression profile of whole CLN and peripheral LN from naive mice revealed a difference in gene expression of IgG2b, which correlated with an increased number of IgG2b-producing plasma cells in the CLN compared with peripheral LN (16). We hypothesize that such mucosa-specific Ig expression may differentially modulate DC function by selective engagement of Ig FcR (17). FcR constitute a family of surface molecules expressed on hemopoietic cells that are capable of stimulating or inhibiting cellular responses upon being immobilized by their ligand, Ab-Ag complexes (18, 19). Types I and III FcR for IgG (FcRI and FcRIII) are expressed primarily on cells of the myeloid lineage and can mediate effector functions such as phagocytosis, Ab-dependent cytotoxicity, and release of inflammatory mediators (18). Signaling via these receptors leads to activating signals through intracellular ITAM motifs (20). In contrast, the type II receptor (FcRII), which is found on both myeloid and lymphoid cells, provides inhibitory signals via ITIMs (21). When stimulated, DC can express both activating FcRIII and inhibitory FcRIIB receptors. Therefore, the functional outcome of immune complex-induced stimulation should reflect a balance between opposing stimulatory and inhibitory signals. The implications of such a balance for subsequent CD4+ T cell responses have not yet been elucidated. However, absence of FcRII renders mice more susceptible to inducible forms of autoimmunity and spontaneous autoimmunity, suggesting that this receptor plays a role in establishing tolerance to self Ags (18, 19). Based on these findings, we questioned whether, in the mucosa-draining LN, signaling via FcRIIB plays a role in inhibiting Ag presentation and DC maturation, leading to Tr induction and subsequent mucosal tolerance to exogenous Ag.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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BALB/c mice were obtained from Charles River Laboratories and kept in our animal facility under routine laboratory conditions. Eight- to 10-wk-old FcRIIB–/– mice on 129/C57BL/6 background and wild-type (WT) controls were provided by S. Verbeek (Leiden University Medical Center, Leiden, The Netherlands). FcRIIB–/– mice on BALB/c background were obtained from Taconic Farms. OVA-specific TCR transgenic mice on BALB/c background (DO11.10 mice) were bred at the Vrije Universiteit University Medical Center. All experiments performed were approved by the Animal Experiments Committee of the Vrije Universiteit University Medical Center.
Mucosal tolerance induction and delayed-type hypersensitivity (DTH) reaction
For induction of nasal tolerance, FcRIIB–/– mice and WT controls received three doses of 100 µg of OVA protein (OVA type VII; Sigma-Aldrich)/10 µl of saline intranasally on each of 3 consecutive days. For oral tolerance induction, mice received a single feed of 25 mg of OVA intragastrically. The development of tolerance was read out by sensitizing the mice 8 days after the last intranasal or oral administration by injecting 100 µg of VA/25 µl of saline/25 µl of IFA (Difco Laboratories) s.c. in the tail base. As challenge for the DTH response, 10 µg of OVA/10 µl of saline was injected 5 days later in the auricle of each ear. The increase of ear thickness was measured with an engineer’s micrometer (Mitutoyo) at 24 h after the challenge and compared with the ear thickness, as measured before OVA injection. Measurements were performed in blinded fashion. Values are expressed as the mean increase in ear thickness of both ears at 24 h postchallenge.
Flow cytometry
Single cell suspensions of CLN from WT and FcRIIB–/– were prepared by straining the tissues through a 100-µm gauze. LN cells, or DC that had been cultured in vitro, were washed in PBS containing 2% heat-inactivated FCS (BioWhittaker) (FACS buffer), and aliquots were incubated with Abs: rat anti-CD86 (clone GL-1), rat anti-CD40 (clone 3/23), FITC-conjugated rat anti-MHCII (clone M5/114), PE-conjugated rat anti-CD80 (clone 1G10), PE-conjugated hamster anti-CD11c (HL3), rat anti-B220 (clone 6B2), and rat anti-
TCR (clone H57-597) at the appropriate concentrations. Supernatant obtained from the murine K9361 hybridoma (22) specific for the Ly-17.1 allele of the FcRIIB, a kind gift of M. Daeron (Institut Curie, Paris, France), was purified, biotinylated according to manufacturer’s protocol (Molecular Probes), and used to detect FcRIIB. Subsequently, the cells were washed three times with FACS buffer and, when conjugate staining was necessary, the cells were incubated on ice for 30 min with PE-conjugated donkey anti-rat Ig (The Jackson Laboratory) or streptavidin-PE (Vector Laboratories), as appropriate. After incubation, the cells were washed and resuspended in FACS buffer, and fluorescence was measured using a FACSCalibur (BD Biosciences). A total of 50,000, 70,000, or 250,000 events per sample was analyzed. Cells that had been incubated with concentration matched isotype control and, when required with conjugate, served as negative controls.
DC culture
Bone marrow-derived DC (BM-DC) were cultured from BALB/c or FcRIIB–/– mice. Briefly, on day 0, femurs and tibia of adult donor mice were flushed, and the resulting BM suspension was passed through a 100-µm gauze to obtain a single cell suspension. The cells were seeded at 2 x 106 per petri dish in IMDM (Invitrogen Life Technologies) supplemented with 10% heat-inactivated FCS, 50 U/ml sodium penicillin-G (BioWhittaker), 5 x 10–5 M 2-ME (Merck), and 20 ng/ml murine rGM-CSF (X63-GM-CSF-producing cell line supernatant (23)). On days 3 and 6, 20 and 10 ng/ml murine rGM-CSF was added, respectively, in 10 ml of fresh IMDM. On day 8, the nonadherent cells consisting of immature and mature BM-DC were harvested and used for subsequent experiments.
In vitro Ig stimulation and FcRIIB neutralization
Twenty-four-well plates (Greiner Bioscience) were coated with purified, endotoxin-low, mouse IgG1 anti-trinitrophenyl (BD Pharmingen) at 10 µg/ml in PBS, or PBS alone as a control, at 37°C. After a 60-min incubation, the coating solution was removed and DC were seeded in the wells at 1 x 106/ml. In several experiments, FcRIIB signaling was neutralized by first preincubating the DC in suspension with K9361 mAb or the isotype control mAb ED6 that stains rat stromal reticular cells and has no known reactivity against mouse tissues (24), both at a concentration of 10 µg/ml in PBS on ice for 60 min. After incubation, the cells were centrifuged, resuspended in IMDM, and seeded in the coated 24-well plates at 1 x 106/ml in the presence or absence of 10 µg/ml K9361 or ED6. After a 60-min incubation, the cells were stimulated with LPS from Escherichia coli 0111:B4 (Difco Laboratories) at a concentration of 1 ng/ml. The cells were cultured at 37°C and 5% CO2, and after 24 h, the supernatant was collected and stored at –80°C, and the cells were recovered for further analysis.
Kinetics of OVA-specific T cell response in vitro and in vivo
OVA-TCR transgenic T cells were isolated and labeled, as described previously (10, 11). Briefly, at 8–12 wk of age, DO11.10 transgenic mice were sacrificed, and LN and spleens were collected. Single cell suspensions of spleens and LN were prepared separately by straining the tissues through a 100-µm gauze. Erythrocytes in the splenocyte suspension were lysed by incubation with lysis buffer (150 mM NH4Cl, 1 mM NaHCO3, pH 7.4) for 5 min on ice. Cell suspensions were depleted of B cells, CD8+ T cells, and APC by incubation with a mixture of mAb containing anti-B220, anti-CD8, MAC-2, F4/80, and anti-MHC-II, and subsequently removing labeled cells with sheep anti-rat IgG Dynabeads, according to manufacturer’s instructions (Dynal Biotech). Thereafter, the remaining CD4+ T cell-enriched fraction was labeled with CFSE (Molecular Probes). The proportion of OVA-TCR transgenic+ CD4+ T cells in suspension was determined by flow cytometric analysis of an aliquot of cells using the clonotypic Ab KJ1-26 (anti-OVA transgenic TCR) and anti-CD4 (GK1.5). For in vitro T cell differentiation studies, 2 x 105 CFSE-labeled KJ1-26+CD4+ T cells were cocultured with 1 x 105 BM-DC in the presence or absence of 100 nM OVA323–339 peptide. At 72 h postculture, T cells and supernatants were harvested for analysis.
For in vivo T cell differentiation studies, the cells were diluted to 1 x 107 KJ1-26+CD4+ T cells/100 µl in saline. Naive FcRIIB–/– or WT acceptor mice (on BALB/c background) were injected i.v. with 1 x 107 KJ1-26+CD4+ T cells. One day after adoptive transfer of KJ1-26+CD4+ T cells, mice were tolerized by administration of 15 µl of saline containing 400 µg of OVA (type VII; Sigma-Aldrich) intranasally. At 48 h posttolerization, nose-draining CLN were removed, and single cell suspensions were analyzed for cell division by flow cytometry.
Measurement of cytokine-producing cells and cytokine release
The percentage of OVA-specific T cells secreting IL-2, IL-4, IL-10, and IFN- was determined using the appropriate murine cytokine secretion assays (Miltenyi Biotec), according to manufacturer’s instructions.
The concentrations of murine MCP-1, TNF-, IL-6, IL-2, IL-10, IFN-, and IL-12p70 in the culture supernatants were determined using a cytometric bead array from BD Pharmingen. Reagents for the IL-12p40 ELISA were obtained from BioSource International. For staining of intracellular IL-12, single cell suspensions of CLN were prepared, as described earlier. The cells were first stained for CD11c and thereafter fixed and permeabilized with the Cytofix/Cytoperm Plus kit with Golgistop (BD Pharmingen) and stained with PE-labeled rat anti-mouse IL-12 p40/p70 (clone C15.6) or PE-labeled rat-IgG1 isotype control (clone R3-34), according to manufacturer’s instructions.
Adoptive transfer of total or subsets of CD4+ splenocytes from tolerized mice to naive recipients
FcRIIB–/– or WT acceptor mice (on BALB/c background) were tolerized via the nasal mucosa and sensitized for a DTH response, as described above. Measurement of the DTH response confirmed that WT mice had become tolerant, whereas FcRIIB–/– mice had not. Seven days after challenge, spleens from WT and naive control mice were collected. Single cell suspensions were obtained by mincing the spleens and straining them through 100-µm gauze. The cell suspensions were enriched for CD4+ T cells, as described above. CD4+-enriched cells were adoptively transferred to naive BALB/c acceptor mice by the i.v. injection of 5 x 105 CD4+ T cells. For transfer of CD4+CD25+ and CD4+CD25– T cells, CD4+ cells were labeled with biotinylated anti-CD25 Ab (clone PC61; BD Pharmingen) for 20 min on ice, followed by anti-biotin immunomagnetic beads (MACS; Miltenyi Biotec) for 20 min at 4°C, and separated using an MS column, according to the manufacturer’s protocol. Purified CD4+CD25+ (98% CD25+) and CD4+CD25– (98% CD25–) T cells were transferred at 2 x 105 cells/mouse. One day after adoptive transfer, all mice were sensitized, and 5 days thereafter, mice were challenged according to the DTH protocol described above.
Statistics
For ear-swelling responses, the mean increase in ear thickness of both ears was determined for each mouse per group. For all experiments, groups were compared by ANOVA, followed by a two-sided Tukey-Kramer multiple comparisons test. A p value of <0.05 was considered significant.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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RIIB is essential for mucosal tolerance
We recently detected a difference in gene expression of IgG2b in the CLN compared with peripheral LN of naive mice, which correlated with an increased number of IgG2b-producing plasma cells in the CLN (16). If such differential Ig expression is involved in modulation of DC function mediating tolerance at mucosa-draining sites, it would require a role for Ig FcR. We therefore established whether the inhibitory FCR, FcRIIB, regulates mucosal tolerance induction. FcRIIB–/– mice and WT controls received intact OVA via the nasal mucosa, and were subsequently subjected to a standard systemic DTH challenge (9, 11) to assess whether tolerance to OVA had developed. In contrast to WT controls, FcRIIB–/– mice were not able to suppress a DTH response after nasal pretreatment with OVA, as can be seen from Fig. 1A, in which comparable increases in ear thickness in FcRIIB–/–-tolerized and nontolerized groups are seen.
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RIIB–/– mice by ingestion of OVA via the gastrointestinal tract was also assessed. As shown in Fig. 1B, the intragastric ingestion of OVA by FcRIIB–/– mice also failed to induce suppression of a DTH response to OVA, whereas WT mice became tolerant.
In conclusion, these findings show that FcRIIB is essential for mucosal tolerance induction via both nasal and oral routes.
Absence of FcRIIB function on BM-DC alters Ag presentation, leading to an altered T cell response in vitro
FcRIIB functions by providing inhibitory signals through ITIMs (21). The balance between inhibitory signals from FcRIIB and stimulatory signals from FcRI and FcRIII may determine the outcome of the response of DC to exogenous Ag: tolerance or activation. We therefore investigated whether the demonstrated requirement for FcRIIB in the establishment of nasal and oral tolerance (Fig. 1) could be due to the inhibitory effect of FcRIIB on DC phenotype and function.
Immature BM-DC from FcRIIB–/– and WT mice were therefore stimulated in vitro with immobilized IgG1, and either neutralizing anti-FcRIIB Ab or isotype control, where appropriate, and subsequent changes of costimulatory molecule expression and cytokine secretion were investigated. Stimulation was elicited by the presence of low doses (1 ng/ml) of LPS to mimic priming of BM-DC as occurs upon Ag encounter in vivo.
Stimulation with immobilized IgG1 in the presence of LPS significantly increased the expression of CD80 (data not shown), MHC class II (data not shown), CD86, and CD40 on BM-DC from both FcRIIB–/– and WT mice when compared with DC incubated with LPS alone (Fig. 2, A and B). However, when the signaling via the FcRIIB on DC was either absent or neutralized using the mAb K9361, stimulation with immobilized IgG and LPS dramatically further increased the expression of CD86 and CD40 expression (Fig. 2, A and B), whereas the expression of CD80 and MHCII was only slightly or not affected (data not shown). LPS priming was not essential to demonstrate the induction of enhanced costimulatory molecule expression on FcRIIB-deficient BM-DC, as incubation with immobilized IgG with and without OVA also led to enhanced CD86 and tended to increase CD40 expression in FcRIIB-deficient BM-DC (Fig. 2D). The up-regulation of costimulatory molecules was accompanied by an enhanced secretion of the inflammatory cytokines MCP-1, TNF-, and IL-6 in the absence of FcRIIB function (Fig. 2C).
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RIIB on BM-DC did not affect the number of dividing cells nor their kinetics of division relative to observations for WT BM-DC (Fig. 3A). However, the division of FcRIIB–/–-BM-DC-stimulated T cells was associated with a vast increase in IL-2 secretion, and a slight, but consistently enhanced IFN- release, relative to the division of WT-BM-DC-stimulated T cells (Fig. 3B).
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RIIB function enhances BM-DC function, with consequent changes in the elicited T cell response. The inhibitory function of FcRIIB in this respect is clear, in line with its requirement in the generation of nasal and oral tolerance.
Altered DC activation in the nose-draining CLN of FcRIIB–/– mice results in a different T cell response
We next investigated whether the in vitro observed enhancement of BM-DC activation and resulting altered T cell response would lead to similar functional changes in the in vivo generation of nasal tolerance. We have previously shown that mucosal Tr differentiate from naive T cells in the nose-draining CLN within 72 h post-Ag application (11). Within that time, the Tr attain all requirements for their suppressive function, even though their phenotype is highly similar to that of nonregulatory T cells (11). We therefore hypothesized that any balancing effect of FCRIIB on Ag presentation required for mucosal Tr induction would be exerted in the mucosa-draining CLN within the first 72 h post-OVA treatment. In CLN, both DC and B cells express FcRIIB irrespective of saline or OVA treatment (data not shown).
Indeed, at 24 h postnasal OVA application, secretion by DC of the inflammatory cytokines TNF-, IL-6, and IL-12p70 was significantly higher in the CLN of FcRIIB–/– mice than WT mice (Fig. 4, A and B). This increase in secretion was not due to enhanced DC influx into the node, as total numbers of CD11c+ cells in the CLN were comparable for FcRIIB–/– and WT mice (data not shown). Furthermore, based on the number of CD11c+ cells within the node that expressed IL12p40, and the amount of this cytokine released in the culture, it was calculated that the amount of IL-12p40 released per CD11c+cell was significantly enhanced in FcRIIB–/– mice (Fig. 4C). MCP-1 secretion also tended to increase (Fig. 4A), but this was not significant.
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RIIB–/– mice, no increased up-regulation of costimulatory molecules on DC in the CLN could be detected in vivo, as demonstrated by the finding that at 24 h post-OVA the mean fluorescence intensity (MFI) of CD40 (2172.9 ± 224.4) and CD86 (3259.9 ± 22.4) on CD11c+ cells in WT mice was comparable to the MFI of CD40 (2100.0 ± 131.7) and CD86 (3307.6 ± 194.9) in FcRIIB-deficient mice. These data suggest that local IgG in the mucosa-draining LN modulates DC activity in an FcRIIB-dependent manner.
Having established that enhanced DC activation occurs in the CLN of intranasally treated FcRIIB–/– mice, we proceeded to investigate the consequences for the resulting T cell response. A possible change in the OVA-specific T cell response was assessed by adoptively transferring FcRIIB–/– mice and WT mice on BALB/c background with OVA-specific TCR transgenic (DO11.10) CD4+ T cells that had been labeled with CFSE. One day later, these mice were treated with OVA intranasally, and 72 h thereafter, the kinetics of division of CFSE-labeled OVA-specific T cells in the CLN were measured. OVA-specific CD4+ T cells of both WT and FcRIIB–/– mice had undergone between zero and four divisions (Fig. 5A). The percentage of OVA-specific T cells within each division did not differ between WT and FcRIIB–/– mice, indicating that the kinetics of division of OVA-specific T cells was not altered (Fig. 5A), as was also observed in Fig. 3A.
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RIIB–/– mice, we determined the percentage of CD25+ OVA-specific T cells among the dividing cells in the CLN. As shown in Fig. 5B, there was no difference in the percentage of CD25+-dividing OVA-specific T cells in the CLN of the FcRIIB–/– mice, excluding that there is a complete lack of these cells. We are cautioned when drawing this conclusion by the fact that at this time point of differentiation expression of CD25 may also be required to receive an IL-2 signal, irrespective of whether these cells are Tr or effector T cells. Because there are no other markers available to distinguish mucosal Tr from effector T cells, we assessed whether the enhanced DC activation in FcRIIB–/– led to altered differentiation with respect to cytokine secretion. The percentage of cytokine-producing OVA-specific T cells and the release of cytokines by total CLN cells were also determined in WT and FcRIIB–/– mice (Fig. 5, B and C). The percentage of IL-2-secreting OVA-specific T cells was not detectably higher in FcRIIB–/– mice compared with WT mice (Fig. 5B); this finding is reflected by the similar concentrations of IL-2 in the supernatants of total LN cell restimulation cultures from WT and FcRIIB–/– mice (Fig. 5C). However, the percentage of IFN--secreting undivided cells or one-time-divided T cells was significantly higher in FcRIIB–/– mice than in WT mice (Fig. 5B); this increase is reflected by a 4-fold increase in the concentration of IFN- in the supernatant of total CLN cell restimulation cultures of FcRIIB–/– mice compared with WT (Fig. 5C). No IL-4 or IL-10 OVA-specific T cells could be detected in either WT or FcRIIB–/– mice.
In conclusion, these data show that the down-regulating effect of FcRIIB in the induction of nasal tolerance results in a significantly reduced IFN- secretion by Ag-specific T cells, but not in an alteration in IL-2 secretion or kinetics of T cell division.
Absence of functional CD4+ Tr in FcRIIB–/– mice
A decisive step in the development of mucosal tolerance is the induction of Ag-specific Tr that suppress subsequent systemic challenges with the same Ag (3, 4, 5, 6, 7, 8). Previously, we have demonstrated that after Tr differentiation in the mucosa-draining LN, these cells leave the LN and can be isolated from the spleen. These Tr are CD4+ and fall in both CD25+ and CD25– subsets (10, 11). The OVA-specific T cells in the CLN of FcRIIB–/– mice had an increased proinflammatory profile relative to those in WT mice (Fig. 5), and so we hypothesized that as a result of unbalanced Ag presentation and subsequent altered T cell differentiation, no Tr were generated in FcRIIB–/– mice.
To test this hypothesis, FcRIIB–/– mice and WT mice received OVA via the nasal mucosa and were subsequently sensitized and challenged. As predicted by the experiment described in Fig. 1, FcRIIB–/– mice did not become tolerant, whereas WT mice did (data not shown). One week after challenge, CD4+ T cells were isolated from the spleens of WT and FcRIIB–/– mice and transferred to naive recipients. Transfer of tolerance by the CD4+ T cells to the naive recipients was assessed by sensitizing and challenging the latter for a DTH response, and comparing the outcome with that of mice that were merely sensitized and challenged (positive DTH control). As depicted in Fig. 6A, mice that received CD4+ T cells from tolerized WT mice were tolerant, as exhibited by a significantly lower ear thickness than control DTH mice. However, mice that had received CD4+ T cells from OVA intranasally treated FcRIIB–/– mice were not tolerant, as evidenced by an increase in ear thickness similar to that of the positive DTH control (Fig. 6A). To assess whether lack of FcRIIB affects a specific subset of Tr-purified splenic CD4+CD25+ and CD4+CD25– cells from WT and FcRIIB–/– mice were transferred separately. As shown in Fig. 6B, function of both subsets derived from FcRIIB–/– mice was perturbed, as they were unable to suppress a DTH response. It should be noted that when compared with the WT CD4+CD25– T cells, the CD4+CD25– T cells from FcRIIB–/– mice seemed to retain some residual suppressive ability (Fig. 6B).
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RIIB balances Ag presentation that is required for both functional CD25+ as well as CD25– Tr induction in the mucosa-draining LN. |
Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
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RIIB, that signaling via this receptor is essential for the induction of nasal tolerance. In vitro, as well as in the nose-draining LN, absence of Ig-initiated signaling via FcRIIB on DC disturbs the normal balance of costimulatory molecule expression and cytokine secretion, resulting in a proinflammatory condition whose outcome is an altered CD4+ T cell differentiation and prevention of functional Tr cell formation. From these results, we conclude that signaling via FcRIIB on DC may prevent maximal costimulatory molecule expression and proinflammatory cytokine secretion, a balance required for Tr induction during the generation of mucosal tolerance.
Our findings are the first evidence that a member of the FcR family is involved in Tr induction in mucosal tolerance. They are in line with the observation that FcRIIB–/– mice are susceptible to both inducible forms of autoimmunity, such as collagen-induced arthritis and Goodpasture’s syndrome (25, 26), and also to spontaneous autoimmunity (27). Although spontaneous autoimmunity due to lack of FcRIIB function can largely be attributed to a defect in B cell function (28), the results presented in this work question a role of FcRIIB in regulating Tr that maintain tolerance to self Ags (29, 30, 31).
What is the mechanism underlying a central role of FcRIIB in mucosal tolerance? The OVA applied on the nasal mucosa is most likely taken up by local DC that line the mucosa, as has been demonstrated for buccal (32) and gastrointestinal (15) mucosal immunizations. Resting DC predominantly express the inhibitory FcRIIB, which accounts for 
75% of total FcR expression (17). However, whether this also holds true for mucosal DC is unclear, although the results reported may imply this is the case. After Ag uptake, the DC migrate to the mucosa-draining LN while undergoing activation and/or differentiation. Upon migration and activation, DC may up-regulate the stimulatory FcRI and FcRIII, which, upon encountering immune complexes, initiates many cellular changes, including the up-regulation of costimulatory molecules and proinflammatory cytokine secretion, resulting in enhanced T cell activation (20); indeed, we have confirmed that WT BM-DC that were stimulated with immobilized IgG enhanced their costimulatory molecule expression and cytokine secretion (Fig. 2). However, immune complexes also trigger signaling of the inhibitory ITIM containing FcRIIB, leading to association of this receptor with activated FcRI and III and down-modulation of their stimulatory ITAM-mediated signals (21). Similarly to our murine experiments, human monocyte-derived DC primarily express the IIB form of FcR, which, upon cross-linking with immobilized IgG, induces DC maturation associated with IL-10 secretion that can be blocked with a neutralizing Ab against FcRII (33). Clearly, our data show that in the absence of FcRIIB function, this down-modulation does not occur, because stimulation of FcRIIB–/– BM-DC with immobilized IgG resulted in increased costimulatory molecule expression relative to WT BM-DC, most notably CD86 and CD40, and substantially enhanced proinflammatory cytokine secretion (Fig. 2). This is in line with a previous report that BM-DC of FcRIIB–/– mice show more MHC class II and CD86 expression than WT BM-DC when stimulated with immune complexes consisting of OVA with anti-OVA rabbit IgG (17). Crucially, this FcRIIB-mediated down-regulation of DC activation has functional consequences in vivo, because the application of OVA via the nose-induced deregulated proinflammatory cytokine release in the CLN of FcRIIB–/– mice, which, in the case of IL-12p70, could be related to increased IL-12p40 release per CD11c+ cell (Fig. 4).
In contrast to the in vitro experiments with myeloid BM-DC, the expression of costimulatory molecules on DC in the CLN was not detectably increased in FcRIIB-deficient mice. This may be explained by the fact that Ag presentation in vivo is more complex, most likely involving DC belonging to different lineages that are either resident in the LN or migrate into the LN after picking up Ag at the mucosa. The response of these cells to OVA is likely to be less homogeneous than in the in vitro system, which may limit detection of the enhanced costimulatory molecule expression.
We have previously shown that increased numbers of IgG2b-producing B cells are present in the mucosa-draining LN of naive mice (16). In the experiments described in this work, it is unclear whether there is an additional increase in IgG that is specifically directed against OVA, especially considering the short period of 72 h after protein administration. Such monomeric IgG and/or immune complexes may modulate the function of the migrated or resident DC by ligation of FcR similarly to the effects described after i.v. Ig treatment (34): independent of its Ag specificity, i.v. -globulin treatment has effective anti-inflammatory activity against a variety of immune-mediated disorders, due to the induction of inhibitory FcRIIB on macrophages (35).
It should be noted that naive FcRIIB–/– LN did not contain resting DC or B cells with enhanced costimulatory molecule expression (data not shown), implying that resting FcRIIB–/– LN DCs have a phenotype comparable to WT DC. It seems therefore that it is the inability of FcRIIB–/– mice to develop tolerance in response to tolerogenic Ag that explains their normal DTH response (Fig. 6), rather than the spontaneous deregulated generation of stimulatory conditions in the absence of tolerogenic Ag.
Characteristics of DC that are associated with the generation of a tolerogenic T cell response include specific subsets of DC, such as CD8-positive DC (36); surface ligands including ICOS-L (37) and receptor activator of NF-
B (38); and/or the degree of maturity of the DC (14). These studies attest that defined conditions of Ag presentation determine whether the outcome of a T cell response is tolerogenic or immunogenic. Therefore, deregulated DC activation in the mucosa-draining LN following OVA treatment in FcRIIB–/– mice could be the basis of the subsequent observed T cell response, from tolerogenic to immunogenic. The effects of deficient FcRIIB signaling on CD4+ responses in vitro and in vivo were characterized in the OVA-specific CD4+ T cell response in coculture or in the nose-draining LN after nasal OVA application, respectively. Surprisingly, no difference in the number of divisions or proportion of dividing OVA-specific CD4+ T cells could be observed in FcRIIB–/– mice (Figs. 3A and 5A).
In vitro, in the absence of FcRIIB on DC, Ag-induced T cell proliferation leads to enhanced IL-2 release, but is not accompanied by enhanced T cell proliferation when compared with T cells incubated with WT DC. This is surprising, as it has been known for a long time that IL-2 has potent T cell growth factor activity in that it generates clonal expansion of T cells. The simplest interpretation of these findings is that the T cells are unable to respond to the higher amounts of IL-2. This seems unlikely, as within each peak of division there are dividing cells that express the IL-2R chain CD25. However, the IL-2 may also have other activities besides affecting the clonal expansion. A more enticing, but speculative explanation is that IL-2 may control effector cell differentiation. Recently, it has been proposed that signaling through the IL-2R can be a crucial checkpoint for substantial clonal expansion and development into effector cells. The IL-2-mediated signal is denoted as signal 3, after ligation of the TCR (signal 1) and engagement of costimulatory molecules (signal 2) during Ag presentation by the DC (39). IL-2 may control effector T cell differentiation by, for example, stabilizing accessibility of cytokine genes, as has been demonstrated for the Il-4 gene (40). Although under homeostatic conditions Tr induction is dependent on IL-2 release (39), the excessive IL-2 release as seen in our model may skew the T cell differentiation away from a Tr phenotype. Whether such a IL-2-mediated regulation may explain the observed IFN- production (Fig. 3B) is unclear.
Although this in vitro system is useful to detect the effect of FcRIIB on DC-T cell interaction, it should be viewed with caution. This is best illustrated by our finding that in vivo enhanced IL-2 secretion was not evident in LN cells from FcRIIB-deficient mice when compared with the in vitro data shown in Fig. 3B. In vivo Ag presentation in the LN takes place within a complex microenvironment in which movement of Ag, APCs, and T cells is restricted to specific anatomical locations. As recently described, there are resident and migrating DC in the LN (41). Both types of DC have the capacity to acquire Ag and generate peptide-MHCII complexes, but may have different functions. Migrating DC have up-regulated CD86/CD80 and may be highly stimulatory T cells, whereas resident DC can be tolerogenic associated with lower expression of costimulatory molecules. In theory, the T cells in the nose-draining CLN could encounter both migrating and resident DC that differ with respect to FcRIIB expression and in consequence have a different status of activation. Moreover, in contrast to the myeloid DC used in vitro, such CLN-DC may also belong to other lineages such as the plasmacytoid DC, as found in human nasal mucosa (42), and in murine lungs, where this similar DC phenotype was shown to have different T cell-stimulating properties (43). Due to this complexity of DC-T cell interaction in vivo, the enhanced IL-2 release may possibly not be detected or may be overruled by inhibitory signals from the natural environment.
Crucially, both in vitro and in vivo absence of FcRIIB on DC favored the development of IFN--secreting T cells: in particular, more IFN--producing cells were observed, and more IFN- protein was detected in the supernatant of total LN cell cultures after restimulation with Ag (Figs. 3B and 5C). These findings suggest a skewing of the T cell response toward an immunogenic Th1-like phenotype and away from a tolerogenic Tr phenotype.
What are the consequences of these changes toward a more Th1-like proinflammatory response to OVA in the mucosa-draining lymph node of FcRIIB–/– mice? The differentiation of mucosal Tr within 72 h of nasal OVA application is inhibited. We have previously demonstrated that after differentiation in the mucosa-draining LN, Tr reside within the CD4+ splenic T cell pool in both the CD25+ and CD25– compartments (9). Therefore, we assessed whether these splenic regulatory T cells could be retrieved from FcRIIB–/– mice. Undeniably, our data show that CD4+ splenic T cells from tolerized FcRIIB–/– mice fail to transfer tolerance to naive mice and that both CD4+CD25+ as well as CD4+CD25– Tr are affected in their function, clearly demonstrating that there is abortive Tr induction in FcRIIB–/– mice.
In summary, we report new evidence that signaling via FcRIIB is essential for Tr induction in the generation of mucosal tolerance. Signaling via FcRIIB on DC in the mucosa-draining LN, initiated by local IgG, down-regulates costimulatory molecule expression and cytokine secretion induced by stimulatory FcRI and FcRIII, resulting in the defined conditions necessary for Tr induction and subsequent tolerance as opposed to immune response. Further studies on the regulation of FcRIIB signaling will be important for understanding the intricate processes that underlie Tr induction and will have consequences for application of tolerance induction in autoimmune disease and allergy.
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1 Address correspondence and reprint requests to Dr. Janneke N. Samsom at the current address: Laboratory of Pediatric Gastroenterology, Erasmus MC, Room EE1571A, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands. E-mail address: j.samsom{at}erasmusmc.nl 
2 Current address: Genomics Laboratory, Department of Physiological Chemistry, University Medical Center Utrecht, Postbus 85060, 3508 AB Utrecht, The Netherlands.
3 Abbreviations used in this paper: Tr, regulatory T cell; BM-DC, bone marrow-derived DC; CLN, cervical LN; DC, dendritic cell; DTH, delayed-type hypersensitivity; LN, lymph node; MFI, mean fluorescence intensity; WT, wild type.
Received for publication September 27, 2004. Accepted for publication February 15, 2005.
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) controls by the intranasal administration of OVA on each of 3 consecutive days (A). Oral tolerance was induced by giving mice a single feed of 25 mg of OVA intragastrically (B). Controls received saline via respective routes. The establishment of tolerance was assessed by sensitizing the mice with OVA/IFA s.c. in the tail base and 5 days later injecting 10 µg of OVA protein in the auricle of each ear as a challenge DTH response. The increase of ear thickness was measured at 24 h after the challenge and compared with the ear thickness as measured before OVA injection. Values are expressed as the mean increase in ear thickness of both ears of one mouse at 24 h postchallenge relative to the mean ear thickness before challenge (n.s. = nonsignificant). These data are representative of three separate experiments.
= WT; 
= Fc
