Files in this Data Supplement:

• Video 1 (QuickTime, 1,778 KB) - RAW 264.7 macrophages expressing GFP-ubiquitin were treated with 1 μg/mL LPS from S. typhimurium for 8 h. Coverslips were then mounted onto an inverted Leica DMIRE2 spinning disk confocal microscope with a heated stage and an Orca AG CCD camera. Approximately 27 confocal images were acquired at different focal planes (0.38 μm optical slices) through each cell at a given timepoint. Images were acquired every 90 seconds through the duration of each experiment. All images were obtained with cells kept at 37°C in RPMI media buffered to pH 7.2 with HEPES. As shown, ubiquitinated protein aggregates move rapidly in the cytosol and undergo fusion after coming into contact with each other.
• Video 2 (QuickTime, 2,185 KB) - RAW 264.7 macrophages expressing GFP-ubiquitin were treated with 1 μg/mL LPS from S. typhimurium for 8 h and visualized as in Video 1. Where indicated, nococazole (5 mM) was added to cells to disrupt microtubules. This treatment rapidly blocked the movement of DALIS within cells.