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* Kuzell Institute for Arthritis and Infectious Diseases, San Francisco, CA 94115;
Department of Internal Medizin II Infectious Diseases, University of Freiburg, Freiburg, Germany;
Experimental Facilities Division, Argonne National Laboratory, Argonne, IL 60439;
Tuberculosis Research Section, Laboratory of Host Defenses, National Institutes of Health, Rockville, MD 20852; and
¶ Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
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Abstract |
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or TNF-
before infection altered the concentrations of elements in the phagosome, which was not observed in cells activated following infection. Siderophore knockout M. tuberculosis vacuoles exhibited retarded acquisition of iron compared with phagosomes with wild-type M. tuberculosis. This is a unique approach to define the environmental conditions within the pathogen-containing compartment. |
Introduction |
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Mycobacterium tuberculosis and Mycobacterium avium are important human pathogens responsible for the death of millions of individuals yearly (7, 8). Both M. tuberculosis and M. avium are intracellular pathogens that infect primarily mononuclear phagocytes. When inside macrophages and monocytes, M. tuberculosis and M. avium reside within cytoplasmic vacuoles that neither acidify nor fuse with lysosomes (9, 10). The phagosomal environment likely influences the expression of virulence genes of M. tuberculosis or M. avium.
It is well known that iron is a required element for growth and survival of M. tuberculosis in its host (11), and iron overload can be an exacerbating cofactor to tuberculosis (12, 13). Not only is iron important for growth but either iron or manganese is also part of the active center of stress-resistance proteins such as superoxide dismutase (SOD),4 a known scavenger of oxygen radicals. SOD has been described in pathogenic M. avium and M. tuberculosis (14, 15). However, it is unclear whether the iron concentration in the mycobacterial phagosome in macrophages is limiting, as documented previously for Salmonella-containing vacuoles in epithelial cells. Researchers have tended to infer that the concentration of iron in phagosomes containing Mycobacterium spp. is limiting because of the extensive array of iron-binding moieties expressed by the bacterium (16, 17). However, the documented delivery of transferrin to Mycobacterium-containing vacuoles calls this interpretation into question (18, 19, 20).
To directly measure the concentration of the single elements such as iron within the mycobacterial phagosome, we used a novel technique, the hard x-ray microprobe with suboptical resolution. This system enables mapping of the trace element in a specimen by analyzing characteristic x-ray fluorescence spectra without the use of a fluorescence dye. Autoradiography of infected macrophages incubated with 59Fe-loaded transferrin confirmed the measured increase of the iron concentration in the mycobacterial vacuole, supporting the hypothesis that the vacuolar iron is available to mycobacteria.
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Materials and Methods |
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M. avium strain 101, a clinical isolate from the blood of an AIDS patient, has been previously described (21). M. tuberculosis H37Rv was purchased from American Type Culture Collection, Mycobacterium smegmatis mc2155 was kindly provided by W. Jacobs, Jr. (Albert Einstein School of Medicine, Bronx, NY), and the mutant H37Rv 01A was created as previously described (16). The bacteria were cultured as previously reported (16) on Middlebrook 7H11 agar.
Specimen preparation
Peritoneal macrophages from C57BL/6 (bcgS) mice were isolated as described (21, 22) and grown for 18–24 h on sterile, formvar-coated London finder gold grids (Electron Microscopy Sciences) in RPMI 1640 supplemented with 10% FBS (Sigma-Aldrich). The monolayers were infected with M. avium (strain 101), M. tuberculosis (strain H37Rv), the siderophore knockout mutant of M. tuberculosis strain H37Rv (Mtb H37Rv O1A) or M. smegmatis (strain mc2 155) for 1 h with an approximate ratio of 10 bacteria per macrophage. The viability of the inoculum was determined by using the LIVE-DEAD (Molecular Probes) assay as previously reported (21). Only inocula with at least 90% viability were used for the assays. After 1 and 24 h of infection, the macrophages were washed twice with HBSS to remove the remaining extracellular bacteria and fixed in 1% paraformaldehyde (pH 7.2) for 30 min. The electron microscopy grids were then washed in HBSS twice, rinsed shortly with sterile water, air-dried, and then kept at room temperature in viewing chambers for the microscopic identification of bacteria or phagosomes. Recombinant murine IFN- and recombinant murine TNF- (purchased from BioSource) were used to stimulate the macrophages. Treatment of peritoneal macrophages with 102 U/ml IFN- or 102 U/ml TNF- were performed 24 h before or 24 h after infection with M. avium. Macrophage monolayers remained exposed to cytokines for 24 h. Extracellular bacteria that were not removed by the washing were fixed after 1 h of infection and used as a source of extracellular bacteria for the measures.
Hard x-ray microprobe
This technique directly (physically) measures the elemental concentrations, combining high elemental sensitivity (subfemtogram) and low background with suboptical spatial resolution (150 nm). The hard x-ray microprobe (23) that was used to collect maps of the elemental distribution in the macrophage and the phagosome operates at the Advanced Photon Source, a third generation synchrotron that produces tunable hard x-rays with high intensity and collimation in a small spectra band. Direct excitation of atomic K or L transitions of all elements in the periodic system is possible by tuning the incident x-ray energy to a value between 6 and 30 keV. We chose an incident energy of 10 keV, which allowed efficient excitation of elements with a Z number up to 30 (Zn) at highest spatial resolution. Due to absence of bremsstrahlung from inelastically scattered charged particles, as is encountered in electron or proton microprobes, the background in the x-ray microprobe is significantly smaller, yielding an intrinsic sensitivity of better than micromolar. Use of monochromatic x-rays for excitation confines background from elastic and inelastic photon scattering to the high-energy side of the detected x-ray spectra, where it can easily be separated from the fluorescence signal that is unique to individual elements. Biological specimens as thick as 50–100 µm can be penetrated at sub-200-nm resolution, without any widening of the probing beam, as is encountered in electron microprobes. Therefore, no physical sectioning of the specimen is needed. In fact, whole macrophages are examined here without any sectioning. To map the elemental distribution, the specimen is placed on a motorized scanning stage, and raster scanned through the x-ray spot. An energy-dispersive ultra-LEGe detector (Canberra) with a spectral resolution of 180 eV is used to record the fluorescence spectra at each specimen point. A schematic representation of the hard x-ray beamline with the microprobe is shown in Fig. 1. Depending on the concentration of the element in question and specimen-intrinsic background, dwell times of 1–20 s are required for each pixel. In the simplest data analysis mode, spectra obtained at each pixel are filtered around the energies of K radiation of the elements of interest, allowing simultaneous mapping of currently up to 10 elements. The resulting elemental composition is mapped against the x and y position to yield an elemental map of the specimen. By normalizing the measured signal with data obtained from a calibration standard, fully quantitative maps of the elemental distribution are obtained for potassium, calcium, manganese, iron, copper, and zinc. For elements such as phosphorus, sulfur, chlorine, and nickel, where no calibration standards are available at the moment, relative concentrations are used.
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The macrophage and the region containing the phagosome were preselected using the optical microscope and specific coordinates were assigned to the vacuole using finder grids and the same kinematic mounts in the optical microscope and in the microprobe. A coarse scan on the macrophage, taken with a pixel size of 1 x 1 µm at large photon flux (
2 x 109 photons/s) allowed the superimposition of the microscopic image with the scan and, together with the assigned coordinates, clear identification of the region containing the phagosomes. Then, a high resolution scan of the phagosome, with a pixel size of usually 0.2 x 0.2 µm and correspondingly reduced photon flux (2 x 108 photons/s) was taken, yielding 10 elemental maps.
Data analysis
Independent of the mycobacterial species, comparison of the microscopic image and the scans showed that the bacterial shape was outlined by the chlorine or the potassium distribution. These elements were therefore used as one of the parameters to precisely define the phagosomal/bacterial region of interest. In the same scan, a background region was defined next to the respective phagosome, omitting structures of the macrophage either visible in the light microscopic image or detected in the different images of the scan that could have adversely influenced the background counts. The mean count rate of this background was subtracted from the mean count rate of the phagosomal/bacterial region to yield the mean phagosome/bacteria count rate. The mean counts were normalized with synchrotron current and exposure time. For quantitation of elemental concentrations, thin-film standards with a known concentration of the respective element were available (National Institute of Standards and Technology/National Bureau of Standards (NBS) 1832, NBS 1833) were used. The yield of absolute atomic concentration in area density (nanograms per square centimeter) for elements (potassium, calcium, iron, copper, manganese, and zinc) using the molar mass of the respective elements and assuming the median thickness of mycobacteria to be 1 µm, these data can be expressed as millimoles per liter or micromoles per liter. For elements where no standard was available (phosphorus, sulfur, chlorine, and nickel), the normalized signal in counts per second per A was used for further analysis. As a control, we used latex beads. Macrophages were allowed to take up latex beads, and macrophage monolayers were fixed and prepared 1 and 24 h after uptake, as described above. The concentration of elements on the beads was measured and calculated as follows: region of interest (RO1) for the latex beads was defined with identical geometry. One RO1, with diameter of 1 µm, was placed in the center. RO2 was centered around the latex bead. A thickness of 200 nm was taken into consideration. Statistical analysis was performed using Student’s t test in the InStat computer program. A value of p < 0.05 was considered significant. The number (n) of mapped phagosomes per group is indicated in the tables.
Incubation with iron transferrin
Mouse bone marrow-derived macrophage monolayers were infected with M. avium 101, and the infection was left to establish for 4 days. The cultures were incubated overnight in medium with transferrin-depleted serum before experimentation. Human transferrin was stripped of iron and reloaded with 59Fe (Amersham Biosciences) as described previously by Dyer et al. (24) and separated from free 59Fe by two sequential passages over 10-ml gel filtration columns (Pierce). Labeled transferrin (10 µg/ml) was added to the infected macrophage cultures in the presence or absence of 100 µg/ml unlabeled transferrin. The cultures were incubated overnight with the added transferrin and either fixed and processed for electron microscopy or rinsed and chased for 2 days before fixation and processing. The cultures were fixed in 1% glutaraldehyde in PBS, postfixed OsO4, and dehydrated through an ethanol series before embedding in Spurr’s resin. Sections were cut and stained with uranyl acetate and Reynold’s lead before coating with autoradiography emulsion EM1 (Amersham Biosciences), as detailed previously (25). The samples were incubated in the presence of a 10-fold excess of unlabeled transferrin and showed signal levels <5% of the experimental samples.
The relative distribution of specific signal was calculated from 20 negatives taken at x6000 magnification from each condition and time point. The number of decay traces associated with the cell cytoplasm, bacteria, and nuclei were scored, corrected for relative surface area, and displayed as a histogram showing signal density per square micrometer.
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Results |
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We used a hard x-ray microprobe beamline (Fig. 1) to perform a quantitative analysis of the intravacuolar concentrations of 10 single elements. The respective experiments were conducted during several runs during a period of 2 years. Before, during, and after each experiment, the instrument was calibrated using the thin-film standards. The variability of quantification within a run and between different runs was 5–10%. Measurements were conducted in macrophages infected with M. avium, M. tuberculosis, or M. smegmatis at 1 and 24 h following infection. Fig. 2 shows the allocation of the phagosomes; Fig. 3 shows representative elemental maps for four different elements and the respective microscopic image of the mapped phagosome.
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The manganese concentration decreased in M. smegmatis vacuoles from 1 to 24 h after uptake by macrophages and was significantly lower at the later time point when compared with M. avium vacuoles. Changes that were observed in potassium, calcium, and copper concentrations did not reach statistical significance.
As control, macrophages were infected with latex beads, and the concentration of single elements was determined. Table II shows that latex beads do not appear to contain any metal.
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Given the known role of iron in bacterial growth and intracellular survival, we paid particular attention to its concentration within the vacuole. We observed that the concentration of iron in M. smegmatis phagosomes 1 h after bacterial uptake by macrophages was significantly higher in M. avium or M. tuberculosis phagosomes but decreased significantly with time. In contrast, the concentration of iron in both M. avium and M. tuberculosis vacuoles increased significantly between 1 and 24 h following uptake (Table III).
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Comparison of the concentration of other elements between the H37Rv wild-type-containing vacuole and the H37Rv O1A mutant revealed a significantly increased zinc and a significantly decreased chlorine concentration in the mutant vacuoles 1 and 24 h after infection. The zinc concentration in extracellular O1A mutants was also higher than in the wild type, however, without being statistically significant (p = 0.07, data not shown). The calcium concentration was higher in the mutant vacuoles at the 1-h time point. A significant change in the concentrations of any element within the first 24 h of infection was not observed in the mutant vacuoles (Table IV).
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It is known that intracellular M. avium reside in vacuoles that lie within the transferrin recycling pathway of their host macrophage (26), and mycobacteria synthesize siderophores and mycobactins capable of competing iron off transferrin (11). A recent study suggested that the internalized transferrin constitutes a source of iron that is associated with intracellular M. tuberculosis. However, it has not been formally shown that the iron is exploited and internalized by the bacterium (19). The distribution of 59Fe can be determined through analysis of the traces appearing in the photographic emulsion layered over the sections examined under the electron microscope. Analysis of M. avium-infected cells following 16 h of continuous incubation with [59Fe]transferrin revealed that the majority of internalized 59Fe was associated with structures within the cytoplasm of the cell, as illustrated in Fig. 4A; although, even at this time point, many of the bacteria were positive for 59Fe (Fig. 4B). When the data were corrected for the relative difference in surface area and expressed as the number of radioactive decays detected in a 10-µm2 area of either cell cytoplasm, nuclei, or bacteria, it became clear that, even at 16 h, there was a disproportionate amount of label associated with the bacteria (Fig. 5). This enrichment of 59Fe was even more marked at 48 h following a 32-h chase in the absence of additional labeled transferrin, and 59Fe was observed with even greater frequency among the intracellular M. avium. These data indicate that M. avium acquires iron from transferrin and retains it at a concentration higher than that observed in the host macrophage cytosol.
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or TNF- on the intravacuolar concentration of elements
Activation of the host macrophage by macrophage-activating cytokines has been shown previously to lead to increased acidification and maturation of Mycobacterium-containing vacuoles while the bacteria are still viable (27). To determine the effect of macrophage activation on the concentration of elements, we used the following two procedures: 1) macrophages were stimulated with IFN- or TNF- for 24 h and then infected with M. avium for 24 h; and 2) macrophages were infected with M. avium for 24 h and then stimulated with recombinant cytokines for an additional 24 h.
TNF- treatment altered the concentration of elements in M. avium phagosomes compared with their concentrations in untreated macrophages. The phosphorus and sulfur concentrations decreased, and the zinc concentration increased if TNF- was added to infected macrophages but did not change if TNF- was added before infection. The decrease in the chlorine concentration when TNF- was added after infection was not significant (p = 0.07). TNF- treatment prevented the increase of the iron concentration in M. avium-containing phagosome if added before infection and reversed the increase of the iron concentration seen in phagosome after 24 h of infection when added to M. avium-infected macrophages (Table V).
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had different consequences on the concentration of elements in the phagosome when compared with untreated controls: phosphorus, sulfur, and chlorine concentrations were decreased, and zinc concentration was increased by IFN- treatment after infection but did not alter when IFN- was added before infection. The potassium concentration was higher, and the calcium concentrations were lower with IFN- treatment before infection when compared with vacuoles in which the cytokine was added to infected macrophages. This notably differs from the effect of TNF- treatment after infection. The nickel concentration decreased, and the copper concentration increased with IFN- treatment regardless of time point of treatment. The increase that was observed of copper with IFN- treatment was significantly higher, when the cytokine was added after infection (Table VI).
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Our data were obtained by measuring the concentration of each element in a number of different vacuoles. Among the observations made was that different vacuoles infected with the same number of bacteria of the same species demonstrated heterogeneity in the concentrations of certain elements. This heterogeneity is likely a product of several factors. First, not all the bacteria in the inocula are viable, or at the same metabolic level, as evidenced by the heterogeneity observed in extracellular bacteria in culture medium. Second, the modulation of the phagosome by the bacteria is also heterogeneous within a range, as demonstrated by the measurements of individual vacuole pH published by Oh and Straubinger (30) but also noted by Clemens and Horwitz (18) for the acquisition of transferrin and for the lysosome-associated membrane glycoprotein CD63. As many as 40% of the M. tuberculosis phagosomes lack staining for transferrin, and half of these stained richly for CD63 (18). Nonetheless, despite the variation between vacuoles, the trends from mycobacterial species of varying pathogenicity are clear and reproducible.
Among our findings, most notable was the observation that although the concentration of iron in M. smegmatis phagosomes significantly decreased with time after bacterial ingestion by macrophages, the concentration of iron in both M. avium and M. tuberculosis vacuoles increased between 1 and 24 h following uptake. Considering the importance of iron for the survival of living organisms, this finding suggests that the pathogenic mycobacteria, in contrast to the nonpathogenic mycobacteria, possess mechanisms aimed at increasing the concentration of iron in the phagosome.
The bacteria’s success in iron acquisition is demonstrated in our study using radioactive iron-loaded transferrin, which showed that extracellular iron is incorporated by infected macrophages and is delivered to M. avium vacuoles through the activity of the transferrin receptor. This observation confirms the findings from recently published studies (19) and the findings obtained by x-ray microscopy that indicate that vacuoles of virulent mycobacteria have the capacity to access and retain iron. In normal phagosome biogenesis, the phagosome shows transient access to the rapid recycling pathway and transferrin (26). The non-acidified phagosomes of M. avium or M. tuberculosis are arrested in their normal maturation, but remain fusion competent and fuse with vesicles of the early endosomal system. At pH 6.3, the pH of the sorting endosome and of the M. avium-containing vacuole (10), iron dissociates from the iron-saturated holotransferrin, enabling the apotransferrin receptor complex to recycle to the plasmalemma. However, even in the absence of this release step, Horwitz and colleagues (11) have shown that the siderophores of M. tuberculosis have an affinity for iron high enough to compete the metal off transferrin at neutral pH. The iron acquisition molecules, siderophores, and mycobactins are thought to compete with the host’s mechanisms to diminish the vacuolar iron concentration. A role for siderophore in iron acquisition was demonstrated in the recent work by De Voss et al. (16), who demonstrated that mycobactin-deficient M. tuberculosis were impaired for intracellular survival. Our data show that the absence of siderophores in this knockout mutant have a direct impact on the ability of the mycobacteria to acquire or retain intravacuolar iron, supporting the hypothesis that the vacuolar iron is available to mycobacteria, and siderophores are important to prevent the host from pumping iron out of the phagosome.
In macrophages that were activated with IFN- before the infection, the accumulation of iron after 1 day of infection was prevented in the phagosomes of the pathogenic mycobacteria. IFN- is known to down-regulate the expression of the transferrin receptor and to decrease the intracellular labile iron pool. It is also known that once macrophages have been activated with IFN-, they can acidify the M. avium vacuoles to pH 5.2 and mature further down the endosomal continuum (27). This transition enhances the microbicidal capacity of the macrophage (31). However, the inability to induce a change in iron concentration in macrophages treated with IFN- after infection is likely a reflection of the reported anergy that infected macrophages show to activating IFN- (32). It is interesting to note that the intravacuolar concentrations of single elements in macrophages activated with IFN- or TNF- show variation between elements such as chlorine, calcium, nickel, and copper. Also, the fact that TNF- in contrast to IFN- was capable of reversing the accumulation of iron in the mycobacterial phagosome after infection suggests that the mechanisms or degrees of macrophage activation by both cytokines are not comparable.
Little is known about the concentration or regulation of other elements in the phagosomes of intracellular bacteria, although these are crucial for many cell functions. Among other differences that were observed for the concentration of several elements in the phagosome of different mycobacterial species and during macrophage activation, the most notable was the increased zinc concentrations in the siderophore knockout M. tuberculosis strain. It may be that, in this mutant, Zn2+ is used as a substitute for metabolic purposes in storage proteins and enzymes for the unavailable Fe2+, as suggested by the higher zinc concentrations in extracellular mutant bacteria. However, the fact that a similar increase in the zinc concentration was observed in the presence of TNF- or IFN- in infected macrophages implies specific regulatory mechanisms for the delivery of this element to the mycobacterial phagosome. In this respect, the observation that a Cu,Zn-SOD contributes to the resistance of M. tuberculosis against oxidative burst products generated by activated macrophages is intriguing (14).
Possible candidates for this sequestering of iron and/or other divalent cations from the endosomal system into the cell cytosol are members of the divalent cation transport family of proteins, natural resistance-associated macrophage protein 1 (NRAMP1; which now is classified as the solute carrier family 11 member 1, Slc11a1) and NRAMP2 (DMT1), which are expressed in macrophages at different stages of the endosomal continuum (33, 34). Colocalization of mycobacterial phagosomes has only been shown with the NRAMP1 protein in some M. avium-containing phagosomes (34), but whether NRAMP1 functions as an efflux pump or as an influx pump is an ongoing debate (35, 36, 37). NRAMP1 recruitment may increase the fusogenic properties of the mycobacterial phagosome (35) and affects intracellular survival of M. avium in bone marrow-derived mouse macrophages after an initial lag period of 2–4 days (38), possibly by the modulation of the phagosomal pH (39). In our study, NRAMP1s mice were used, and the question whether and how the presence of a functional NRAMP1 protein affects the phagosomal concentration of single elements was not addressed.
Localization of NRAMP2 to early endosomes and its colocalization to the transferrin receptor in the mouse monocyte-macrophage cell line RAW 264.7 (33) implies that the NRAMP2 transporter may be present in the mycobacterial phagosome at earlier stages than NRAMP1. Concordantly, infection of mice peritoneal macrophages with M. avium has been shown to up-regulate the expression of Nramp2-mRNA several hours before the Nramp1-mRNA expression. Simultaneously, the synthesis and expression of transferrin receptor RNA was decreased (40). It remains to be seen whether NRAMP1 or NRAMP2 function as Fe2+, Mn2+, or Zn2+ transporter (41) or even as a Cu2+ or Ni2+ transporter in the mycobacterial phagosome and whether these are involved in the changes in the concentration of respective cations in phagosomes of virulent and avirulent mycobacteria, as observed in our study.
In conclusion, this study provides the first exact measurements of the intraphagosomal concentrations of single elements, a notable gap in our knowledge of the luminal content of the endosomal/lysosomal system that has a major impact on the health and well-being of intracellular pathogens. Most notably, our results suggest that pathogenic mycobacteria but not the avirulent M. smegmatis accumulate iron in the phagosome at least in part through the activity of the transferrin receptor and that the siderophore production is necessary to retain the iron in the mycobacterial phagosome. In context with the observation that the siderophore-deficient mutant of M. tuberculosis is markedly attenuated for intramacrophage survival (16), this study highlights the importance of the iron concentration for the intracellular survival of pathogenic mycobacteria.
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Acknowledgments |
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Footnotes |
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1 This work was supported by Grant ROI-AI 47010 of the National Institutes of Health and by the U.S. Department of Energy, Office of Basic Energy Sciences, under Contract W-31-109-Eng-38. D.W. was supported in part by a scholarship of the Walter-Marget-Wereinigung, Germany. 
2 D.W. and J.M. contributed equally to this study.
3 Address correspondence and reprint requests to Dr. Luiz E. Bermudez, Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, 105 Magruder Hall, Corvallis, OR 97331. E-mail address: Luiz.Bermudez{at}oregonstate.edu
4 Abbreviations used in this paper: SOD, superoxide dismutase; NRAMP, natural resistance-associated macrophage protein.
5 Current address: Tulane University, School of Medicine, Department of Microbiology and Immunology, New Orleans, LA 70112.
Received for publication August 18, 2003. Accepted for publication November 16, 2004.
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2-microglobulin knockout mice by lactoferrin abolishes their increased susceptibility to tuberculosis. J. Exp. Med. 196:1507. and hemochromatosis. J. Biol. Chem. 277:49727. transcriptional responses without inhibiting activation of STAT1. J. Immunol. 163:3898.Related articles in The JI:
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A. J. Harrison, M. Yu, T. Gardenborg, M. Middleditch, R. J. Ramsay, E. N. Baker, and J. S. Lott The Structure of MbtI from Mycobacterium tuberculosis, the First Enzyme in the Biosynthesis of the Siderophore Mycobactin, Reveals It To Be a Salicylate Synthase. J. Bacteriol., September 1, 2006; 188(17): 6081 - 6091. [Abstract] [Full Text] [PDF]
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D. Hofreuter, J. Tsai, R. O. Watson, V. Novik, B. Altman, M. Benitez, C. Clark, C. Perbost, T. Jarvie, L. Du, et al. Unique Features of a Highly Pathogenic Campylobacter jejuni Strain. Infect. Immun., August 1, 2006; 74(8): 4694 - 4707. [Abstract] [Full Text] [PDF]
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S. J. Shin, C.-w. Wu, H. Steinberg, and A. M. Talaat Identification of Novel Virulence Determinants in Mycobacterium paratuberculosis by Screening a Library of Insertional Mutants Infect. Immun., July 1, 2006; 74(7): 3825 - 3833. [Abstract] [Full Text] [PDF]
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 D. C. Young and D. B. Moody T-cell recognition of glycolipids presented by CD1 proteins Glycobiology, July 1, 2006; 16(7): 103R - 112R. [Abstract] [Full Text] [PDF]
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R. Appelberg Macrophage nutriprive antimicrobial mechanisms J. Leukoc. Biol., June 1, 2006; 79(6): 1117 - 1128. [Abstract] [Full Text] [PDF]
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 T. M. Doherty and P. Andersen Vaccines for Tuberculosis: Novel Concepts and Recent Progress Clin. Microbiol. Rev., October 1, 2005; 18(4): 687 - 702. [Abstract] [Full Text] [PDF]
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