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Cutting Edge: Bcl-3 Up-Regulation by Signal 3 Cytokine (IL-12) Prolongs Survival of Antigen-Activated CD8 T Cells¹

Center for Immunology, Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455

	Abstract

Top Abstract Introduction Materials and Methods Results and Discussion References

 
Clonal expansion of T cells requires cell division and survival during the proliferative phase of the response. Naive murine CD8 T cells responding to Ag and costimulation undergo an abortive response characterized by impaired clonal expansion, failure to develop effector functions, and long-term tolerance. A third signal provided by IL-12 is required for full expansion, activation, and establishment of memory. The enhanced survival, and thus clonal expansion, supported by IL-12 is not due to increased Bcl-2 or Bcl-x_L expression; both are maximally activated by signals 1 and 2. In contrast, Bcl-3, recently shown to enhance survival when ectopically expressed in T cells, is increased only when IL-12 is present. Furthermore, examination of Bcl-3-deficient CD8 T cells demonstrates that the increased survival caused by IL-12 depends upon Bcl-3. The time courses of expression suggest that Bcl-2 and Bcl-x_L promote survival early in the response, whereas Bcl-3 acts later in the response.

	Introduction

Top Abstract Introduction Materials and Methods Results and Discussion References

 
Survival of activated T cells is tightly regulated to insure that adequate clonal expansion occurs to control infections, but that sufficient death occurs to prevent autoimmune responses and shrink expanded populations following Ag elimination. Bcl-2 and Bcl-x_L, antiapoptotic members of the Bcl-2 family of proteins, promote survival of T cells, and are up-regulated in response to Ag and costimulation. Adjuvants can also play a critical role in regulating survival of T cells (1, 2), but do not do so by increasing expression of the Bcl-2 family members (3, 4). Mitchell et al. (3) have recently shown that expression of Bcl-3 is up-regulated in Ag-stimulated T cells in an adjuvant-dependent manner and that ectopic expression of Bcl-3 could increase survival of activated T cells, suggesting that this might be a mechanism by which adjuvants increase clonal expansion. Their studies did not define how Bcl-3 gene expression is induced by adjuvants, but they suggested that this might involve the inflammatory environment induced by the adjuvants (3).

Curtsinger et al. (5) demonstrated that Ag and B7-1-dependent costimulation (signals 1 and 2) of naive CD8 T cells is not sufficient to stimulate optimal clonal expansion or development of effector functions, but that both occur if IL-12 is present to provide a third signal. Production of IL-12 by APC is one of the components of the inflammatory response induced by many adjuvants, and IL-12 could replace adjuvant in supporting a strong in vivo response by CD8 T cells to peptide Ag (6, 7). Administration of Ag in the absence of adjuvant results in some cell division but clonal expansion is limited, and the cells develop little if any effector function and become tolerant to restimulation (1, 6, 7, 8). When IL-12 is provided along with Ag, strong clonal expansion occurs, effector function develops, and a responsive memory population results (6, 9). Clonal expansion is promoted, in part, by IL-12 enhancing survival of the cells (6). These effects of IL-12 suggested that it might act in part by up-regulating Bcl-3 expression and the results described here demonstrate that this is the case.

	Materials and Methods

Top Abstract Introduction Materials and Methods Results and Discussion References

 
Stimulation and survival of naive CD8 T cells

OT-I mice (10) were housed in a specific pathogen-free environment. C57BL/6 mice were from National Cancer Institute (Frederick, MD). FVB;129P2-BCL-3^tm1Ver Bcl-3-deficient (11) and control FVB mice were from The Jackson Laboratory. Naive CD8 T cells were purified by negative selection (12). Artificial APC were prepared by coating microspheres with 2C11 anti-CD3 mAb (BD Pharmingen) at 1.0 µg/10⁷ beads, or with DimerX H-2 K^b:Ig fusion protein (BD Pharmingen) at 1.0 µg/10⁷ beads. Peptide was loaded onto the H-2K^b by incubating microspheres with 0.1 µM OVA_257–264 for 2 h at 37°C. Murine B7-1-Fc (R&D Systems) was coimmobilized at 0.2 µg/10⁷ beads. Ag and B7-1 densities were in the range shown to be effective for T cell stimulation (13).

Responder CD8⁺ T cells (5 x 10⁴) and Ag-coated microspheres (10⁵) were placed in flat-bottom microtiter wells and cultured at 37°C (5). Where indicated, human rIL-2 (2.5 U/ml; R&D Systems), mouse rIL-12 (2.0 U/ml; Genetics Institute, Cambridge, MA), and/or mouse rIL-1 (5 ng/ml; R&D Systems) were added. Proliferation was determined by adding [³H]TdR during the final 8 h of culture. For in vitro survival experiments, equal numbers of live CD8⁺ T cells that had been cultured for 3 days in wells coated with Ag (0.1 µg/well) and B7 (0.05 µg/well) were washed, labeled with CFSE, and returned to culture in the absence of Ag or cytokines. Survival was measured by counting trypan blue-negative live cells.

To measure survival in vivo, OT-1 CD8⁺ T cells were activated for 3 days in vitro and CFSE labeled (above). Cells were then adoptively transferred by i.v. injection (tail vein) into Thy 1.2⁺ C57BL/6 recipient mice. OT-1 cells remaining 2–3 days later were identified and quantitated by gating on Thy1 or CD45 congenic markers on the OT-I cells.

Western blot and RT-PCR

Expression of mRNA for Bcl-2, Bcl-3, Bcl-x_L, and -actin was determined using a semiquantitative two-step RT-PCR assay. PCR amplification was done using primers designed with Primer3 software (14). Experiments showed that 27 cycles allowed for detection, and normalization to -actin, within the linear range of amplification. For detection of Bcl-3 by Western blotting, whole-cell lysates from 3 x 10⁶ cells were run on SDS-PAGE gels, transferred to a nitrocellulose membrane and blotted using anti-Bcl-3 Ab (Santa Cruz Biotechnology), and detected using an HRP-conjugated secondary Ab.

	Results and Discussion

Top Abstract Introduction Materials and Methods Results and Discussion References

 
To determine the requirements for up-regulation of Bcl-3, naive CD8 T cells were purified from C57BL/6 mice and stimulated in vitro with microspheres having immobilized 2C11 anti-CD3 mAb, with IL-2 alone or along with IL-12 or IL-1. IL-1 was compared with IL-12, because previous work had shown that IL-1 could replace the need for adjuvant in stimulating CD4 T cells, whereas IL-12, but not IL-1, could replace adjuvant for CD8 T cell responses (5). After 72 h, Bcl-3 mRNA and protein levels were examined. A low level of Bcl-3 mRNA was detected in unstimulated cells, and increased little if at all upon stimulation with anti-TCR and either IL-2 or IL-2 and IL-1 (Fig. 1A). In contrast, there was a large increase when IL-12 was present. In three independent experiments, addition of IL-12 caused a 5.0 ± 0.8-fold change in mRNA expression level, whereas IL-1 caused no significant change. Similar results where obtained when Bcl-3 protein levels were examined by Western blotting (Fig. 1, B and C). In five independent experiments, IL-12 caused a similar increase in Bcl-3 protein, whereas IL-1 caused no significant increase in the four experiments where it was examined. Based on densitometric scans, with background subtracted, the average increase in Bcl-3 protein expression in cells stimulated in the presence of IL-2 and IL-12 was 3.2 ± 0.1-fold in comparison to cells stimulated with only IL-2, and the increase was 3.3-fold in the experiment shown in Fig. 1B.

View larger version (51K): [in this window] [in a new window]   FIGURE 1. IL-12 up-regulates Bcl-3 expression in activated naive CD8 T cells. A, Purified naive CD8 T cells were stimulated with microspheres coated with 2C11 anti-CD3 mAb. IL-2 was added alone, or along with IL-I or IL-12, as indicated. Cells were harvested after 72 h and analyzed for Bcl-3 and -actin mRNA expression by RT-PCR. B and C, Cells were stimulated as in A, and Bcl-3 protein expression was analyzed after 72 h by Western blotting. The specificity of the anti-Bcl-3 polyclonal Ab was confirmed by preincubating the Ab with a blocking peptide for 30 min. Numbers indicate intensity of bands relative to that of naive cells without peptide blocking.

View larger version (47K): [in this window] [in a new window]   FIGURE 2. Effects of B7.1 costimulation and IL-12 on Bcl-3, Bcl-2, and Bcl-xL mRNA expression. Microspheres coated with 2C11 anti-CD3 mAb or Kb/OVAp either alone or with B7.1-Ig were used to stimulate naive OT-1 cells in the presence of IL-2 and IL-12, as indicated. A, Proliferation of CD8 T cells responding to 2C11 mAb. Identical results were obtained when DimerX Kb/OVAp was used as the stimulus (not shown). B, Cells were harvested at 72 h and analyzed for Bcl-3, Bcl-2, and Bcl-xL mRNA expression by RT-PCR. C, Cells were harvested every 24 h for a period of 3 days and analyzed as in B. D, Expression of Bcl-3, Bcl-2, and Bcl-xL mRNA was analyzed by RT-PCR as in C, and the bands were quantitated by densitometry. mRNA abundance is expressed relative to actin (x10).

The experiment shown in Fig. 2B examined mRNA expression levels at 72 h, late in the response when proliferation is maximal and lytic effector function has developed. To determine how expression is regulated during the response, naive OT-I cells were stimulated with K^b/OVAp and examined at 24, 48, and 72 h (Fig. 2C). Bcl-2 and Bcl-x_L mRNA levels increased strongly by 24 h and then declined, and IL-12 had little or no effect. Some up-regulation of Bcl-3 mRNA occurred early in the absence of IL-12, but declined by 72 h. In contrast, IL-12 stimulated strong and sustained up-regulation of Bcl-3 mRNA levels. Fig. 2D shows the time course for mRNA expression levels for the survival proteins in response to K^b/OVAp/B7 and IL-12, the condition that leads to maximal clonal expansion and effector function. Thus, Bcl-2 and Bcl-x_L mRNAs are up-regulated early in the response by signals 1 and 2 and then decline, whereas Bcl-3 mRNA expression is strongly up-regulated only by IL-12, and is sustained late in the response.

Ectopic expression of Bcl-3 enhances survival of activated T cells (3), suggesting that IL-12 might sustain survival at later times to support maximal clonal expansion by up-regulating Bcl-3 expression. To determine whether IL-12 does provide a survival advantage, we examined survival of OT-I T cells following activation with K^b/OVAp/B7 for 72 h, when expansion has peaked and the cells begin to die (data not shown). To examine survival in the absence of continued exposure to Ag, cells were activated for 72 h in microtiter wells having immobilized K^b/OVAp/B7. Cells were then harvested, washed to remove cytokines, and placed in secondary cultures in fresh medium, and the number of live cells remaining was determined over 2 days. We consistently found that cells stimulated in the presence of IL-12 for the first 72 h survived better during the subsequent 2-day period in culture (Fig. 3A). To determine whether greater cell recovery in the secondary cultures might be due to more proliferation during this 2-day period, cells were labeled with CFSE at the end of the 72-h primary culture. Dilution of CFSE showed that the cells undergo one to two additional rounds of division following transfer to secondary cultures, but this did not differ for cells initially grown in the presence or absence of IL-12 (Fig. 3B). Thus, differential recovery during the secondary culture reflects differences in the ability of the cells to survive.

View larger version (27K): [in this window] [in a new window]   FIGURE 3. Naive CD8 T cells activated in the presence of IL-12 have enhanced survival upon Ag and cytokine withdrawal. A, Naive OT-I CD8 T cells were activated in primary cultures using plate-bound Kb/OVAp and B7.1 plus IL-2, in the presence or absence of IL-12. After 72 h, cells were harvested and washed, and CFSE-labeled, and equal numbers of live cells (40 x 103) were transferred to secondary cultures lacking Ag or cytokines. The numbers of live cells remaining posttransfer are shown as the average and SD of triplicate samples. Results are representative of three independent experiments. B, Cells in A were analyzed by flow cytometry at 30 h posttransfer to determine CFSE levels. Open histograms are CFSE levels of cells immediately following CFSE labeling. C, Naive OT-I Thy 1.2+CD45.1+ CD8 T cells were stimulated in vitro in the absence of IL-12, and OT-I Thy 1.1+CD45.2+ cells were stimulated in the presence of IL-12. After 72 h, cells were harvested and washed, labeled with CFSE, mixed in equal numbers, and adoptively transferred into C57BL/6 (Thy 1.2+CD45.2+) recipients (0.8 x 106 of each cell type). After 48 h, cells were harvested from the indicated organs, and the numbers of OT-I Thy 1.2+CD45.1+ and OT-I Thy 1.1+CD45.2+ cells were determined by flow cytometry. Similar results were obtained in an independent experiment, and in three additional experiments examining lymph nodes and spleens only. D, CFSE dye dilution in the OT-I cells described in C. Shaded histograms are CFSE levels of cells immediately following CFSE labeling; gray lines are cells stimulated with IL-2 and IL-12; black lines are cells stimulated with only IL-2; and dotted lines are endogenous CD8 T cells.

To determine whether the survival advantage provided by IL-12 is due to up-regulation of Bcl-3, CD8 T cells from wild-type and Bcl-3-deficient mice were compared. For these experiments, immobilized 2C11 anti-CD3 mAb was used to provide signal 1 for polyclonal activation of the naive CD8 T cells. As expected, activated T cells from FVB wild-type mice survived better in secondary cultures when they received an IL-12 stimulus during the primary culture (Fig. 4A), and this was not due to differential cell division (B). In contrast, the IL-12-dependent survival advantage was lost in cells that lacked Bcl-3.

View larger version (30K): [in this window] [in a new window]   FIGURE 4. IL-12-dependent enhancement of survival requires Bcl-3 expression. Naive CD8 T cells from Bcl-3–/– and wild-type FVB mice were activated in primary cultures using plate-bound 2C11 mAb and B7.1 plus IL-2, in the presence or absence of IL-12. After 72 h, the cells were harvested and washed, and CFSE-labeled, and equal numbers of live cells (40 x 103) were transferred to secondary cultures lacking Ag or cytokines. A, The numbers of live cells remaining posttransfer are shown as the average and SD of triplicate samples. Results are representative of three independent experiments. B, Cells in A were analyzed by flow cytometry at 36 h posttransfer to determine CFSE levels. Open histograms are CFSE levels of cells immediately following CFSE labeling.

The Bcl-2 and Bcl-x_L survival factors for activated T cells are up-regulated by TCR engagement and costimulation, with signal 3 having little or no effect (Fig. 2), in agreement with a previous report showing that IL-12 does not affect expression of these proteins (16). In contrast, Bcl-3 up-regulation depends on signal 3 and occurs later in the response, with optimal expression at day 3. These results agree well with those of Marrack and coworkers (2) demonstrating that adjuvants have little or no effect on Bcl-2 and Bcl-x_L expression, but strongly up-regulate Bcl-3 (3). The timing of expression suggests a model in which the different survival factors have roles at distinct stages in the response, and in vivo studies of the effects of signal 3 on responses of CD8 T cells are consistent with this (6). Our previous work showed that immunization with peptide Ag alone or along with IL-12 resulted in comparable proliferation and clonal expansion (cell numbers) of Ag-specific CD8 T cells by day 2, suggesting that the cells survive equally well with or without signal 3 at this time, with survival likely being promoted by Bcl-2 and Bcl-x_L. Between days 2 and 3, the cells underwent further proliferation to a comparable extent but cell numbers did not increase unless IL-12 was provided, suggesting that survival was compromised late in the response in the absence of the third signal (6), and thus in the absence of Bcl-3 up-regulation.

Differing expression kinetics of survival factors is likely related to the fact that T cells undergo a series of distinct pro- and antiapoptotic programs during a response (17). Early in the response TCR- and Fas/TNFR-dependent death pathways may activate caspase 8, which can be counteracted by the up-regulation of FLIP, a nonenzymatic analog of procaspase 8 (18). A recent study showed that IL-12 can up-regulate FLIP expression in CD8 T cells (16). This could contribute to enhanced early survival, although we do not see large signal 3-dependent differences in clonal expansion at early times, and the Fas pathway may not contribute substantially to the death of activated T cells (19). Later in the response, cytokine withdrawal leads to activation of death pathways, which can be counteracted by the action of the antiapoptotic Bcl-2 family members (17, 19). Bcl-2 is up-regulated by IL-2, and its down-regulation toward the end of the response (Fig. 2) may coincide with IL-2 depletion, consistent with decreased levels of Bcl-2 in T cells at their peak of activation (20, 21). It appears that Bcl-3 becomes a critical survival factor at this time (Figs. 3 and 4).

Unlike Bcl-2 and Bcl-x_L, Bcl-3 is unlikely to act directly to inhibit apoptosis. Rather, Bcl-3 is a member of the IB protein family and can interact with p50 and p52 NF-B complexes to repress or induce gene transcription (22, 23). Thus, Bcl-3 may regulate expression of one or more genes involved in survival, which could include up-regulation of antiapoptotic proteins or down-regulation of proapoptotic proteins such as Bim (19) or other members of the Bcl-2-related family of proteins. Most studies examining the role of NF-B complexes in T cell survival have focused on NF-B heterodimers containing the canonical p65 (relA) subunit (24, 25). Our results and those of Mitchell et al. (3, 4) suggest that NF-B complexes that depend upon Bcl-3 transactivation may also play critical roles. In addition to regulating survival genes, Bcl-3 may regulate genes required for other signal 3 effects on CD8 T cells including effector functions, avoidance of tolerance, and acquisition of memory. Experiments are in progress to examine genes whose expression is regulated by Bcl-3.

	Acknowledgments

 
We thank Susanna Winston for expert technical assistance.

	Footnotes

 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ This work was supported by National Institutes of Health Grants AI34824 and AI35296 (to M.F.M.).

² Current address: Department of Biological Sciences, Columbia University, New York, NY 10027.

³ Address correspondence and reprint requests to Dr. Matthew F. Mescher, Center for Immunology, Box 334 Mayo, 420 Delaware Street SE, Minneapolis, MN 55455. E-mail address: mesch001{at}umn.edu

Received for publication September 10, 2004. Accepted for publication November 1, 2004.

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