| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Research Unit, Saskatchewan Cancer Agency, Departments of Oncology, Microbiology, and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
 |
Abstract |
|---|
 Top Abstract IntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
|---|
|
Introduction |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
|---|
Two models of CD4+ T help for CD8+ CTL responses have been proposed previously, including the passive model of three-cell interaction (3, 4) and the dynamic model of sequential two-cell interactions by APCs (5). The three-cell model suggested that activated CD4+ T cells and naive CD8+ T cells must interact simultaneously with a common APC and that the CD4+ Th cells provide CD8+ T cell help via expression of IL-2 (Fig. 1A). The conundrum, however, is how a rare Ag-specific CD4+ Th cell and an equally rare Ag-specific CD8+ T cell (typically 1 in 105–106 T cells) would simultaneously find the same Ag peptide-carrying APC in an unprimed animal (6). Instead, Ridge et al. (5) have proposed a dynamic model of two sequential interactions, in which an APC first offers costimulatory signals to a CD4+ Th cell and then to a CD8+ CTL cell (Fig. 1B). According to this model, the APC-stimulated CD4+ Th cells must first reciprocally counterstimulate the APCs (through CD40 (CD40L)3 signaling) such that this newly "conditioned" APC can then directly costimulate CD8+ CTLs. Support for this model comprises evidence that Ag-specific CTL responses can be induced by vaccination with either large numbers of APCs activated in vitro through CD40 signaling or, in either MHC class II knockout (KO) or CD4+ T cell-depleted mice, by high level activation of APCs in vivo with anti-CD40 Ab (5, 7, 8, 9). Although this model provides a possible explanation for the conditional nature of T cell help for CTL responses, the experimental conditions used in the above studies may well not accurately model the physiology of Th cell-dependent immune responses in vivo. In addition, a scarcity caveat remains (10), in that very small numbers of Ag-bearing APCs (11) must first activate and be conditioned by the rare naive Ag-specific CD4+ Th cells, and then find and activate in turn equally rare naive Ag-specific CD8+ CTLs. In addition, this model does not explain how Th cells’ IL-2 would be precisely targeted to Ag-specific CD8+ Ag-specific CTLs. Furthermore, the life span of an activated dendritic cell (DC) in the T cell zone of a lymph node is 
48 h (11, 12, 13), possibly due to CD4+ T cell killing of the cognate APCs (14, 15), whereas the Ag-specific CTL response is first detected at about day 5 in the lymph nodes (11, 16). Thus, this dynamic model also does not explain compellingly the temporal gap between Ag presentation and the acquisition of CTL effector function in vivo.
|
|
Materials and Methods |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
|---|
The highly lung metastatic B16 mouse melanoma BL6-10 and OVA-transfected BL6-10 (BL6-10OVA) cell lines were generated in our own laboratory (22). Both cell lines form numerous lung metastasis after i.v. tumor cell (0.5 x 106 cells/mouse) injection. The mouse B cell hybridoma cell line LB27 expressing both H-2Kb and Iab, the mouse thymoma cell line EL4 of C57BL/6 mice, and the OVA-transfected EL4 (EG7) cell line, which is sensitive to CTL killing, were obtained from American Type Culture Collection. Both BL6-10 and BL6-10OVA express similar level of H-2Kb, but not Iab (data not shown). Both BL6-10OVA and EG7 cells expressed OVA by flow cytometric analysis, whereas BL6-10 and EL4 cells did not (Fig. 2). T cell hybridoma cell line RF3370 expresses TCR specific for H-2Kb-OVA peptide complexes (23). The biotin-labeled mAbs specific for H-2Kb (AF6-88.5), Iab (AF6-120.1), CD3 (145-2C11), CD4 (GK1.5), CD8 (53-6.7), CD11b (MAC-1), CD11c (HL3), CD25 (7D4), CD54 (3E2), CD69 (H1.2F3), CD80 (16-10A1), and V
2V
5+ TCR (MR9-4) were obtained from BD Pharmingen. The OVAI (SIINFEKL) and OVAII (ISQAVHAAHAEINEAGR) peptides (24, 25) are OVA tumor peptides for H-2Kb and Iab, respectively, whereas Mut1 (FEQNTAQP) peptide is an irrelevant 3LL lung carcinoma for H-2Kb (26). These peptides were synthesized by Multiple Peptide Systems. The OVA-specific TCR transgenic OT I and OT II mice and H-2Kb, Iab, CD4, CD8, CD54, and CD80 KO mice on a C57BL/6 background were obtained from The Jackson Laboratory. Homozygous OT II/H-2Kb–/–, OT II/Iab–/–, OT II/CD54–/–, and OT II/CD80–/– mice were generated by backcrossing the designated gene KO mice (H-2Kb) onto the OT II background for three generations; homozygosity was confirmed by PCR according to The Jackson Laboratory’s protocols. All mice were maintained in the animal facility at the Saskatoon Cancer Center and treated according to animal care committee guidelines of the University of Saskatchewan.
|
Activated, mature bone marrow-derived DCs, expressing high levels of MHC class II, CD40, CD54, and CD80, were generated from C57BL/6 mice as described previously (27). To generate OVA-pulsed DCs (DCOVA), DCs were pulsed overnight at 37°C with 0.1 mg/ml OVA (Sigma-Aldrich, then washed extensively (26).
Preparation of OT II CD4+ and OT I CD8+ T cells
Naive OVA-specific CD4+ T and CD8+ T cells were isolated from OT II or OT I mouse spleens, respectively, and enriched by passage through nylon wool columns. CD4+ and CD8+ cells were then purified by negative selection using anti-mouse CD8 (Ly2) or CD4 (L3T4) paramagnetic beads (Dynal Biotech) to yield populations that were >98% CD4+/V2V5+ or CD8+/V2V5+, respectively. To generate DCOVA-activated CD4+ T cells, CD4+ T cells (2 x 105 cells/ml) from OT II mice or designated gene-deleted OT II mice were stimulated for 3 days with irradiated (4000 rad) bone marrow-derived DCOVA (1 x 105 cells/ml) in the presence of IL-2 (10 U/ml), IL-12 (5 ng/ml), and anti-IL-4 Ab (10 µg/ml) (R&D Systems) (28). These in vitro DCOVA-activated CD4+ T cells, also referred to herein as CD4+ Th-APCs, were then isolated by Ficoll-Paque (Sigma-Aldrich) density gradient centrifugation or further purified using CD4 microbeads (Miltenyi Biotec) in some experiments. Con A-stimulated OT II CD4+ T (Con A-OT II) cells were similarly generated by incubating splenocytes from OT II or OT II/KO mice with Con A (1 µg/ml) and IL-2 (10 U/ml) for 3 days, after which the CD4+ T cells were purified on density gradients. To ascertain that no DCs were in purified Th-APCs or Con A-OT II cells, these active T cells were further purified by using CD4 microbeads (Miltenyi Biotec).
Phenotypic characterization of DCOVA-activated CD4+ T cells
For the phenotypic analyses, Th-APCs were stained with Abs specific for H-2Kb, Iab, CD3, CD4, CD8, CD11b, CD11c, CD25, CD54, CD69, CD80, and V2V5+ TCR (BD Pharmingen), respectively, and analyzed by flow cytometry. For the intracellular cytokines, cells were restimulated with 4000 rad-irradiated BL27 tumor cells pulsed with OVAII peptide for 4 h (28), and then processed using a Cytofix/CytoPerm Plus with GolgiPlug kit (BD PharMingen), with R-PE-conjugated anti-IL-4, -perforin, and -IFN-
Abs (R&D Systems), respectively. Culture supernatants of the restimulated Th-APCs were analyzed for IFN-, IL-2, and IL-4 expression using ELISA kits (Endogen) as reported previously (26).
In vitro and in vivo membrane molecule transfer assays
In in vitro membrane transfer assay, DCOVA or DCs were incubated with CFSE (0.5 µM) at 37°C for 15 min and washed three times with PBS. CFSE-labeled DCOVA or DCs were incubated with Con A-OT II cells at 37°C for 4 h, then the cell mixtures, the original DCOVA and Con A-OT II cells, were stained with a panel of PE-energy-coupled dye (ECD) Abs specific for H-2Kb, CD54, and CD80, respectively, and analyzed by confocal fluorescence microscopy. CD4+ T cells in the cell mixture were also purified by cell sorting and analyzed by flow cytometry. Con A-OT II cells stained with biotin-labeled isotype-matched Abs and ECD-avidin (BD Pharmingen) were used as controls.
In in vivo membrane transfer assay, naive T cells were isolated from OT II/Iab–/– and OT II/CD80–/– mouse spleens, respectively, and enriched by passage through nylon wool columns. The CD4+ T cells (5 x 106 cells/mouse) were further purified by negative selection using the anti-mouse CD8 (Ly2) paramagnetic beads (Dynal Biotech) and then i.v. injected into wild-type C57BL/6 mice. One group of mice remained untreated. One day subsequent to the injection, another group of mice were i.v. immunized with irradiated (4000 rad) DCOVA (0.2 x 106 cells/mouse). Three days after the immunization, mice were sacrificed. T cells were isolated from the spleens of these two groups of mice and enriched by passage through nylon wool columns. The OVA-specific CD4+ OT II T cells were further purified from these T cells by positive selection using the biotin-anti-TCR Ab and anti-biotin microbeads (Miltenyi Biotec), and then stained with FITC-anti- Iab and FITC-anti-CD80 Abs for flow cytometric analysis, respectively.
Ag presentation
RF3370 hybridoma cells (0.5 x 105 cells/well) were cultured with irradiated (4000 rad) DCOVA or Th-APCs or Con A-OT II (1 x 105 cells/well) for 24 h. To investigate the fate of acquired MHC class I/peptide expression, Th-APCs alone were cultured for 1, 2, and 3 days in culture medium containing IL-2 (10 U/ml), termed Th-APC (1, 2, and 3 days), and then harvested for stimulation of RF3370 cells, respectively. The supernatants were harvested for measurement of IL-2 secretion using an ELISA kit (Endogen).
CD8+ T cell proliferation assays
For in vitro CD8+ T cell proliferation assay, irradiated (4000 rad) stimulators, the Th-APCs, Con A-OT II cells (0.4 x 105 cells/well), DCOVA (0.1 x 105 cells/well), and their 2-fold dilutions, were cultured with a constant number of responders, the naive OT I or C57BL/6 (B6) CD8+ T cells (0.5 x 105 cells/well). To rule out the potent effect of endogenous H-2Kb, Th-APCs generated from H-2Kb–/– OT II T cells were termed Kb–/– Th-APCs and used as stimulator. In some experiments, each of a panel of neutralizing reagents (anti-IL-2, -H-2Kb or -LFA-1 Abs, and CTLA-4/Ig fusion protein) (each 15 µg/ml; R&D Systems) or a mixture of the above reagents were added to the cells, while control cells received a mixture of isotype-matched irrelevant Abs and fusion protein. In other experiments, the irradiated CD4+ Th-APCs and naive OT I CD8+ T cells were cultured in Transwell plates (Costar), separated by 0.4-µm pore-sized membranes. After 48 h, thymidine incorporation was determined by liquid scintillation counting (26).
For the in vivo CD8+ T cell proliferation assay, purified naive OT I CD8+ T cells were labeled with CFSE (1.5 µM) and i.v. injected into C57BL/6 mice (2 x 106 cells each). Twelve hours later, each mouse was i.v. injected with 2 x 106 Th-APCs and Con A-OT II cells, respectively, or 0.2 x 106 DCOVA. In another group, mice were injected with PBS. Three days later, the splenic T cells from the recipients were stained with ECD-anti-CD8 Ab (Beckman Coulter) and then analyzed by flow cytometry.
Cytotoxicity assays
For the in vitro cytotoxicity assay, the activated CD8+ T cells derived from the above 3-day coculture with irradiated (4000 rad) DCOVA, Th-APCs, and Con A-OT II cells were purified on density gradients and termed DCOVA/OT I, Th-APC/OT I, and Con A-OT II/OT I, respectively. These cells as well as Th-APCs were used as effector cells, while 51Cr-labeled EG7, the control EL4 tumor cells, DCOVA, LB27, and OVAII-pulsed LB27 (LB27OVAII) tumor cells were used as target cells, respectively. Specific killing was calculated as: 100 x [(experimental cpm – spontaneous cpm)/(maximal cpm – spontaneous cpm)] as previously described (26).
We adapted a recently reported in vivo cytotoxicity assay (29). Briefly, C57BL/6 mice were i.v. immunized with DCOVA (0.5 x 106 cells), Th-APCs or Con A-OT II cells (2 x 106 cells). Seven days later, mice were boosted once. In another group, mice were injected with PBS. Naive mouse splenocytes were incubated with either high (3.0 µM, CFSEhigh) or low (0.6 µM, CFSElow) concentrations of CFSE to generate differentially labeled target cells. The CFSEhigh cells were pulsed with OVAI, whereas the CFSElow cells were pulsed with the irrelevant 3LL lung carcinoma H-2Kb peptide Mut1 and served as internal controls. These peptide-pulsed target cells were washed extensively to remove free peptide and then i.v. coinjected at 1:1 ratio into the above described immunized mice 3 days after the boost. Sixteen hours after target cell delivery, the spleens were removed and residual CFSEhigh and CFSElow target cells remaining in the recipients’ spleens were sorted and analyzed by flow cytometry.
Animal studies
Wild-type C57BL/6 mice (n = 8) were injected i.v. with 0.2 x 106 DCOVA, 2 x 106 Th-APCs, and Con A-OT II cells, respectively, and then 7 days later they were boosted once. To study the immune mechanism, CD4 and CD8 KO mice (n = 8) were injected i.v. with 2 x 106 Th-APCs and then 7 days later the mice were boosted once. Three days subsequent to the boost, the mice were i.v. given 0.5 x 106 BL6-10OVA or BL6-10 tumor cells. The mice were sacrificed 4 wk after tumor cell injection and the lung metastatic tumor colonies were counted in a blind fashion (22). Metastases on freshly isolated lungs appeared as discrete black-pigmented foci that were easily distinguishable from normal lung tissues and confirmed by histological examination. Metastatic foci too numerous to count were assigned an arbitrary value of >100.
|
Results |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
|---|
To explore DC membrane-derived APC machinery acquisition by CD4+ T cells, Con A-stimulated CD4+ T cells from OVA-specific TCR-transgenic OT II mice were cultured for 4 h. either alone or with DCOVA or DC. The CD4+ T cells were then sorted and examined for expression of MHC class I and II, CD54, and CD80 by flow cytometry. The control Con A-stimulated OT II CD4+ T cells expressed some MHC class I and II, CD54, and CD80. However, following incubation with DCOVA, these T cells displayed moderately augmented levels of these molecules (Fig. 3A), suggesting that DC molecules could have been transferred to the T cells. The membrane transfer can be mostly blocked by addition of anti-H-2Kb and LFA-1 Abs and CTLA-4/Ig fusion protein (data not shown), indicating that the membrane acquisition of Th-APCs from DCOVA is mediated by TCR and costimulatory molecules. In addition, these T cells following interaction with DCs without OVA pulsing also displayed augmented levels of these molecules, but to a lesser extent (data not shown), indicating that these DC molecule transfer is mediated by both the Ag-specific and nonspecific manners.
|
|
, but not IL-4 (Fig. 3C); they also secreted IFN- (2 ng/ml per 106 cells/24 h) and IL-2 (2.5 ng/ml per 106 cells/24 h), but not IL-4, in culture. These data indicate that these OVA-TCR-transgenic CD4+ T cells were Th1. In addition, there was no CD11b+/11c+ DC population existing in these purified CD4+ T cells (Fig. 3C). This is because that any survival of irradiated DCOVA cells and the potential small amount of contamination of spleen DCs or B cells within the original naive OT II CD4+ T cell preparation, which might have picked up OVA peptides from irradiated DCOVA in the culture, would be eliminated by the killing activity of these activated Th1 cells expressing perforin (see Fig. 7B) (35, 36). In addition to the common H-Kb expression, these Th cells also expressed Iab, CD54, and CD80 molecules and here too they did so whether they were derived from wild-type or homozygous H-2Kb–/–, Iab–/–, CD54–/–, or CD80–/– KO mice (Fig. 3D). Thus, we clearly demonstrate that naive CD4+ T cells can also acquire MHC class II and costimulatory molecules (CD54 and CD80) composing the immune synapse as well as the bystander MHC class I from DCs by in vitro DC stimulation.
|
|
If the CD4+ T cell-acquired H-2Kb were in the form of OVAI peptide complexes and the DC costimulatory molecules were functional, then these IL-2-secreting T cells could potentially act as direct APCs (termed CD4+ Th-APCs) for CD8+ T cell stimulation. To examine the functionality of these putative Th-APC cells, we initially assessed their ability to stimulate IL-2 secretion of T cell hybridoma RF3370. As shown in Fig. 6A, RF3370 cells alone did not secret IL-2. However, Th-APCs significantly stimulated RF3370 to secret IL-2 (95 pg/ml) as did DCOVA (220 pg/ml), indicating that Th-APCs expressed functional H-2Kb-OVAI peptide complexes. If the acquired MHC I-OVAI peptide complexes are of any functional significance in vivo, they would need to be stably expressed. The rate of their decay was assessed by culturing these Th-APCs after MHC class I acquisition for varying time periods. As shown in Fig. 6A, the ability to stimulate IL-2 secretion of RF3370 cells did decay over time. However, readily detectable MHC class I/peptide expression was still observed as much as 3 days after in vitro culture.
|
(4.0 ng/ml per 106 cells/24 h) and IL-2 (3.3 ng/ml per 106 cells/24 h), but lacking self-IL-4 and acquired H-2Kb-OVA peptide complexes) stimulate OT I CD8+ T cell proliferation. In addition, Kb–/– Th-APCs derived from the H-2Kb–/– OT II KO mice (Fig. 3D) showed similar CD8+ T cell stimulatory activity as Th-APCs derived from the wild-type OT II mice (Fig. 6B), indicating that the activation of CD8+ OT I T cells is mediated via the acquired H-2Kb-OVA peptide complexes, but not the endogenous H-2Kb of Th-APCs. In separate experiments, we demonstrated that CD8+ T cell stimulatory activity of the Th-APCs was contact dependent since Transwells blocked CD8+ T cell proliferation (Fig. 6C). Furthermore, adding anti-MHC class I or anti-LFA-1 Abs or CTLA-4/Ig fusion protein could significantly inhibit the OT I CD8+ T cell proliferative response in the cocultures by 38, 50, and 58%, respectively, whereas anti-IL-2 Ab had less effect (19% inhibition; p < 0.01). Simultaneous addition of all blocking reagents reduced the proliferative response by 92% (p < 0.01). Taken together, these data indicate that this response is critically dependent on H-2Kb/OVAI/TCR specificity and is greatly affected by nonspecific costimulatory CD54-LFA-1 and CD80-CD28 interactions between the CD4+ Th-APCs and CD8+ T cells. That this proliferative effect was not simply an in vitro artifact was confirmed by demonstrating that these Th1-APCs can also stimulate proliferative responses in vivo. We adoptively transferred CFSE-labeled naive OT I CD8+ T cells into mice that were also given Th-APCs, Con A-OT II cells, DCOVA, or PBS. The labeled CD8+ T cells did not show any division in mice treated with PBS (data not shown). However, the labeled CD8+ T cells underwent some cycles of cell division in the mice given either Th-APCs or DCOVA, but did not respond in the animals given Con A-OT II cells (Fig. 6D). Th-APCs stimulate CD8+ T cell differentiation into CTL effectors in vitro and in vivo
As a critical test of the functionality of these purified CD4+ Th-APCs, we tested their ability to induce the differentiation of naive OT I CD8+ T cells into CTL effectors, as determined using in vitro 51Cr release assays with EG7 tumor cells expressing an OVA transgene. The Th-APC-activated OT I CD8+ T (Th-APC/OT I) cells displayed substantial cytotoxic activity (33% specific killing; E:T ratio, 12) against an OVA-expressing EG7 cell line as did the DCOVA-activated OT I CD8+ T (DCOVA/OT I) cells (46% killing; E:T ratio, 12), but not against its parental EL4 tumor cells (Fig. 7A), indicating that the killing activity of these CTLs is OVA tumor specific. In addition, these CD4+ Th-APCs expressing perforin (Fig. 3C) displayed killing activities for DCOVA and LB27OVAII cells with Iab/OVAII expression (Fig. 7B). However, they themselves did not show any killing activity to LB27 and EG7 (Fig. 7B) or BL6-10OVA cells (data not shown) without Iab/OVAII expression. As with the proliferation assays, the in vitro CD8+ CTL induction capacity of CD4+ Th-APCs can also be translated into an induction of effector CTL function in vivo. We adoptively transferred OVAI peptide-pulsed splenocytes that had been strongly labeled with CFSE (CFSEhigh), as well as the control peptide Mut1-pulsed splenocytes that had been weakly labeled with CFSE (CFSElow), into recipient mice that had been vaccinated with these purified Th-APCs, DCOVA, Con A-OT II cells, or PBS. We assessed the disappearance of the labeled cells from the mice by flow cytometric analysis and found that the CFSElow (irrelevant Mut1 peptide-pulsed) cells were unaffected by the vaccination protocol. In addition, we found that no substantial loss (1%) of the CFSEhigh (OVAI peptide-pulsed) cells from the PBS-immunized mice (data not shown). However, there was substantial loss of the CFSEhigh (OVAI peptide-pulsed) cells from the Th-APC-immunized (86%) or DCOVA-vaccinated (97%) mice, but not from the Con A-OT II cell-vaccinated (2%) mice (Fig. 7C). These data indicate that CD4+ Th-APCs carrying H-2Kb-OVAI complexes and DC costimulatory molecules can stimulate the development of OVA-specific CTL effector cells in vivo.
Th-APCs induce OVA-specific antitumor immunity in vivo
In addition, Th-APCs can also stimulate OVA-specific CTL-mediated antitumor immunity in vivo. We injected these purified Th-APCs i.v. into mice, followed by i.v. challenge with OVA-expressing BL6-10OVA or OVA-negative BL6-10 tumor cells. All mice immunized with Con A-OT II cells (i.e., cells lacking acquired H-2Kb-OVAI complexes and costimulatory molecules) as well as the control mice (eight of eight) without any immunization had large numbers (>100) of lung metastatic tumor colonies 4 wk after tumor cell challenge (Expt. 1 in Table I and Fig. 8). In addition, all mice (eight of eight) immunized with naive OT II T cells also died of lung metastasis (data not shown). However, all mice (eight of eight) immunized with Th-APCs had no lung tumor metastasis. DCOVA immunization was equally effective in inducing antitumor immunity. The specificity of the protection was confirmed with the observation that Th-APCs did not protect against BL6-10 tumors that did not express OVA, with all mice having large numbers (>100) of lung metastatic tumor colonies after tumor cell challenge. To study the immune mechanism, CD4 and CD8 KO mice were used for immunization of Th-APCs. As shown in experiment 2 in Table I, all of the CD4 KO mice (eight of eight) were still protected from BL6-10OVA tumor challenge, indicating that activation of CD8+ CTL response by Th-APCs is independent on the host CD4+ T cells. However, all CD8 KO mice (eight of eight) had numerous lung tumor metastases, indicating that the Th-APCs-driven antitumor immunity is mediated by CD8+ CTLs. The Th-APC-induced CD8+ CTL response is more likely through direct interaction between Th-APCs and CD8+ CTLs rather than cross-presentation of the host DCs picking up OVA peptides released from Th-APCs, because the former is CD4+ T cell independent, whereas the latter is CD4+ T cell dependent.
|
|
|
Discussion |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
|---|
cell OVA Ag and leads to diabetes in rat insulin promoter OVAhigh transgenic mice. They also found that activated CD4+ OT II T cells provide CD40-mediated help to CD8+ T cell responses without these T cells necessarily seeing Ag on the same APC (41). In contrast, some have suggested that CD4+ T cell help is only essential for memory CTL responses (29). Thus, the generation of effectors from naive CD8+ T cells is reported to be helper independent in mice immunized with irradiated embryonic cells expressing an adenovirus type 5 E1A transgene (42). Having said that, it is highly relevant that such adenoviral challenge would also introduce potent inflammatory signals into the sensitizing microenvironment (leading to high level DC maturation) (43), to say nothing of the potential for help from NK cells (44). In addition, the E1A adenoviral Ag features multiple CD8+ T cells epitopes (45) and therefore also a greater base of Ag-specific CD8+ T cell precursors from which to draw (46). A strong and direct activation of DCs (47) would explain the previous demonstrations that induction of some antiviral CTL responses is CD4+ Th cell independent.
T cell-to-T cell (T-T) Ag presentation, dependent upon activated CD4+ T cells first acquiring MHC class II and CD80 molecules from APCs and then stimulating other CD4+ T cells, is increasingly attracting attention (32, 33). However, the roles such T-APCs may play in vivo have been as yet ill-defined and the results of the relevant in vitro studies disparate, in part because multiple experimental systems have been used. For example, CD4+ T-APCs can induce IL-2 production and proliferative responses among naive responder T cells (48, 49), which is consistent with our results in this study. However, these T-APCs have also been shown to induce apoptosis in activated CD4+ T cells or anergization of CD4+ T cell lines (33, 50, 51, 52). In this study, we found that in vivo transfer of CD4+ Th1-APCs expressing high levels of IFN- and IL-2, which were generated by incubation of OT II CD4+ T cells with DCOVA in the presence of IL-12 and anti-IL-4 Ab, were able to stimulate OVA-specific CTL responses. Interesting, we also found that in vivo transfer of CD4+ Th2-APCs expressing high levels of IL-4 and IL-10, which were generated by incubation of OT II CD4+ T cells with DCOVA in the presence of IL-4 and anti-IFN- Ab, were able to induce OVA-specific immune suppression (data not shown). In other reports, however, in vivo transfer of CD4+ Th1-APCs derived from IL-2-dependent transformed T cell lines has been reported to induce immunosuppressive, but not immunostimulatory effects in the context of autoimmune responses (52, 53). In these studies, the T-APCs used were derived from rather uncharacterized Con A-stimulated allogeneic or Ag-pulsed CD4+ T cell lines. Therefore, it is difficult to assess the extent to which they are representative of T-APCs as they would be generated in vivo. In addition, these studies have addressed only the activation of CD4+ T cell responses.
In this study, we have shown that CD4+ T cells can acquire synapse-composed MHC class II, CD54, and CD80 molecules from APCs by APC stimulation. In addition, for the first time, we have shown that CD4+ T cells can also acquire the bystander MHC class I-OVAI peptide complexes which are critical molecules in stimulation of OVA-specific CTL responses. Furthermore, we have provided a complete line of evidence that compellingly substantiates the practical aspects of CD4+ T cells acting as APCs for effective CD8+ T cell responses in vitro and in vivo. A model of CD4+ T cell help for CTL induction that takes these observations into account would address multiple important aspects of this paradigm in cellular immunology. A central caveat in models of CD4+ T cell help for CTL responses is that of scarcity, or how rare Ag peptide-carrying DCs, Ag-specific CD4+, and Ag-specific CD8+ T cells manage to encounter each other with enough efficiency to ensure that we expeditiously and appropriately respond to all Ags/pathogens (i.e., to maintain the integrity of the organism). It is counterintuitive that a function as critical as this not be optimized in some way. The model wherein APCs that are themselves licensed by Th cells to directly activate CD8+ T cells (Fig. 1B) (5) offers the advantage that a single licensed APC can contact multiple CD8+ T cells and thereby expand the activation signal. However, a very limited number of DCs arriving in lymph nodes would interact with many CD4+ T cells, and our evidence demonstrates that they both induce marked proliferative responses among the naive Ag-specific CD4+ T cell population and also bestow on them of these progeny Th-APC functionality. In turn, each new Th-APC can interact with and activate naive CD8+ CTL precursor cells, such that they also undergo expansion. The gain in this system is thereby dramatically increased even before the newly activated CTL precursors begin to proliferate. Other aspects of this new model also fit in well with the practical and theoretical constraints of Th-cell-dependent CTL responses in the host. Experimental evidence clearly shows that provision of IL-2 dramatically augments the efficiency of precursor CTL expansion (2, 3, 4). We have shown that Th-APCs produce IL-2, and our data explain quite simply how CD4+ Th cells’ IL-2 would be efficiently and precisely targeted to Ag-specific CD8+ T cells. It also addresses the requirement for cognate CD4+ T cell help for CD8+ CTL precursors (3, 4, 54), with the APCs in this case being by definition a cognate Th cell.
Taken together, this study clearly delineates the role CD4+ Th-APCs can play in stimulation of CD8+ CTL responses. It also provides a solid experimental foundation for each of the tenants of a new dynamic model of sequential two-cell interactions by CD4+ Th-APCs in Th-cell-dependent CTL immune responses. Not only are Th-APC effective inducers of Ag-specific CTL activity in vitro, but also they efficiently induce protective antitumor immunity in vivo, thereby confirming their physiological relevance. Although we have addressed multiple parameters of this new model in the context of Th cell-dependent CTL responses, in principle its conditions could be equally well met in regulatory T cell-dependent tolerance induction. From our perspective, we are most interested in its impact on the study of antitumor immunity, cancer vaccine development, and other immune disorders (e.g. autoimmunity).
|
Acknowledgments |
|---|
|
Disclosures |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
|---|
|
Footnotes |
|---|
1 This work was supported by Research Grants MOP63259and 67230 from the Canadian Institute of Health Research (to J.X.). Y.L. was supported by Postdoctoral Fellowship Award from the Canadian Institute of Health Research. 
2 Address correspondence and reprint requests to Dr. Jim Xiang, Research Unit, Saskatchewan Cancer Agency, Departments of Oncology, Microbiology and Immunology, University of Saskatchewan, 20 Campus Drive, Saskatoon, Saskatchewan S7N 4H4, Canada. E-mail address: jxiang{at}scf.sk.ca
3 Abbreviations used in this paper: CD40L, CD40 ligand; KO, knockout; DC, dendritic cell; DCOVA, OVA-pulsed DC; LB27OVAII, OVAII peptide-pulsed LB27 cells; ECD, energy-coupled dye.
Received for publication August 30, 2004. Accepted for publication March 25, 2005.
|
References |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionDisclosuresReferences |
|---|
in cytolysis by CD4+, class II MHC-restricted cytotoxic T cells. Immunology 71: 208-212.[Medline]
(TNF-) transgene-expressing dendritic cells (DCs) undergo augmented cellular maturation and induce more robust T-cell activation and anti-tumour immunity than DCs generated in recombinant TNF-. Immunology 108: 177-188.[Medline]
cell antigens. J. Immunol. 172: 5420-5426.This article has been cited by other articles:
|
|
 |
|
  Y. Xie, O. Bai, J. Yuan, R. Chibbar, K. Slattery, Y. Wei, Y. Deng, and J. Xiang Tumor Apoptotic Bodies Inhibit CTL Responses and Antitumor Immunity via Membrane-Bound Transforming Growth Factor-{beta}1 Inducing CD8+ T-Cell Anergy and CD4+ Tr1 Cell Responses Cancer Res., October 1, 2009; 69(19): 7756 - 7766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Koya, H. Matsuda, S. Matsubara, N. Miyahara, A. Dakhama, K. Takeda, and E. W. Gelfand Differential Effects of Dendritic Cell Transfer on Airway Hyperresponsiveness and Inflammation Am. J. Respir. Cell Mol. Biol., September 1, 2009; 41(3): 271 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Umeshappa, H. Huang, Y. Xie, Y. Wei, S. J. Mulligan, Y. Deng, and J. Xiang CD4+ Th-APC with Acquired Peptide/MHC Class I and II Complexes Stimulate Type 1 Helper CD4+ and Central Memory CD8+ T Cell Responses J. Immunol., January 1, 2009; 182(1): 193 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Fisher, P. A. Bulur, S. Vuk-Pavlovic, F. G. Prendergast, and A. B. Dietz Dendritic cell microvilli: a novel membrane structure associated with the multifocal synapse and T-cell clustering Blood, December 15, 2008; 112(13): 5037 - 5045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Ford McIntyre, K. J. Young, J. Gao, B. Joe, and L. Zhang Cutting Edge: In Vivo Trogocytosis as a Mechanism of Double Negative Regulatory T Cell-Mediated Antigen-Specific Suppression J. Immunol., August 15, 2008; 181(4): 2271 - 2275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hao, J. Yuan, S. Xu, M. A. Munegowda, Y. Deng, J. Gordon, Z. Xing, and J. Xiang Antigen Specificity Acquisition of Adoptive CD4+ Regulatory T Cells via Acquired Peptide-MHC Class I Complexes J. Immunol., August 15, 2008; 181(4): 2428 - 2437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L-L. Chiang, J. A. Ledermann, E. Aitkens, E. Benjamin, D. R. Katz, and B. M. Chain Oxidation of Ovarian Epithelial Cancer Cells by Hypochlorous Acid Enhances Immunogenicity and Stimulates T Cells that Recognize Autologous Primary Tumor Clin. Cancer Res., August 1, 2008; 14(15): 4898 - 4907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rapetti, S. Meunier, C. Pontoux, and C. Tanchot CD4 Help Regulates Expression of Crucial Genes Involved in CD8 T Cell Memory and Sensitivity to Regulatory Elements J. Immunol., July 1, 2008; 181(1): 299 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Luketic, J. Delanghe, P. T. Sobol, P. Yang, E. Frotten, K. L. Mossman, J. Gauldie, J. Bramson, and Y. Wan Antigen Presentation by Exosomes Released from Peptide-Pulsed Dendritic Cells Is not Suppressed by the Presence of Active CTL J. Immunol., October 15, 2007; 179(8): 5024 - 5032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hao, J. Yuan, and J. Xiang Nonspecific CD4+ T cells with uptake of antigen-specific dendritic cell-released exosomes stimulate antigen-specific CD8+ CTL responses and long-term T cell memory J. Leukoc. Biol., October 1, 2007; 82(4): 829 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hao, Y. Liu, J. Yuan, X. Zhang, T. He, X. Wu, Y. Wei, D. Sun, and J. Xiang Novel Exosome-Targeted CD4+ T Cell Vaccine Counteracting CD4+25+ Regulatory T Cell-Mediated Immune Suppression and Stimulating Efficient Central Memory CD8+ CTL Responses J. Immunol., September 1, 2007; 179(5): 2731 - 2740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Koya, N. Miyahara, K. Takeda, S. Matsubara, H. Matsuda, C. Swasey, A. Balhorn, A. Dakhama, and E. W. Gelfand CD8+ T Cell-Mediated Airway Hyperresponsiveness and Inflammation Is Dependent on CD4+IL-4+ T Cells J. Immunol., September 1, 2007; 179(5): 2787 - 2796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Cox, A. J. McMichael, G. R. Screaton, and X.-N. Xu CTLs Target Th Cells That Acquire Bystander MHC Class I-Peptide Complex from APCs J. Immunol., July 15, 2007; 179(2): 830 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Adamopoulou, J. Diekmann, E. Tolosa, G. Kuntz, H. Einsele, H.-G. Rammensee, and M. S. Topp Human CD4+ T Cells Displaying Viral Epitopes Elicit a Functional Virus-Specific Memory CD8+ T Cell Response J. Immunol., May 1, 2007; 178(9): 5465 - 5472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. LeMaoult, J. Caumartin, M. Daouya, B. Favier, S. L. Rond, A. Gonzalez, and E. D. Carosella Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells Blood, March 1, 2007; 109(5): 2040 - 2048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Sitati and M. S. Diamond CD4+ T-Cell Responses Are Required for Clearance of West Nile Virus from the Central Nervous System J. Virol., December 15, 2006; 80(24): 12060 - 12069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shi, S. Hao, T. Chan, and J. Xiang CD4+ T cells stimulate memory CD8+ T cell expansion via acquired pMHC I complexes and costimulatory molecules, and IL-2 secretion J. Leukoc. Biol., December 1, 2006; 80(6): 1354 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xia, S. Hao, and J. Xiang CD8+ Cytotoxic T-APC Stimulate Central Memory CD8+ T Cell Responses via Acquired Peptide-MHC Class I Complexes and CD80 Costimulation, and IL-2 Secretion. J. Immunol., September 1, 2006; 177(5): 2976 - 2984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Blachere, H. K. Morris, D. Braun, H. Saklani, J. P. Di Santo, R. B. Darnell, and M. L. Albert IL-2 Is Required for the Activation of Memory CD8+ T Cells via Antigen Cross-Presentation. J. Immunol., June 15, 2006; 176(12): 7288 - 7300. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shi and J. Xiang CD4+ T cell-independent maintenance and expansion of memory CD8+ T cells derived from in vitro dendritic cell activation Int. Immunol., June 1, 2006; 18(6): 887 - 895. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
