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and V
Public Repertoires Are Highly Conserved in Terminal Deoxynucleotidyl Transferase-Deficient Mice1
* Unité de Biologie Moléculaire du Gène-Institut National de la Santé Et de la Recherche Médicale (INSERM) Unité 277-Institut Pasteur, Paris, France;
Cellectis, Romainville, France; and
Laboratoire d’Activation Cellulaire et Transduction des Signaux, Institut de Biochimie et de, Biophysique Moléculaire et Cellulaire, Unité Mixte de Recherche (UMR) 8619 Centre National de la Recherche Scientifique (CNRS), Université Paris Sud, Orsay, France
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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-J and V
-D-J rearrangements. To measure the impact of N-region diversity on public repertoires, we have characterized the TCRs specific for several CD4 or CD8 epitopes of wild-type mice and of mice deficient in the enzyme TdT. We find that V, (D), J usage identified in public repertoires is strikingly conserved in TdT°/° mice, even for the CDR3 loops which are shorter than those found in TdT+/+ animals. Moreover, the 10- to 20-fold decrease in T cell diversity in TdT°/° mice did not prevent T cells from undergoing affinity maturation during secondary responses. A comparison of the CDR3 in published public and private repertoires indicates significantly reduced N-region diversity in public CDR3. We interpret our findings as suggesting that public repertoires are produced more efficiently than private ones by the recombination machinery. Alternatively, selection may be biased in favor of public repertoires in the context of the interactions between TCR and MHC peptide complexes and we hypothesize that MHC helices are involved in the selection of public repertoires. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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TCRs for Ag is generated by various mechanisms (1). The somatic recombination of separate DNA segments, V and J for -chain and V, D, and J for -chain, yields the CDR3 of the TCR. The imprecise joining of these gene segments, the addition of template-dependent (P) and template-independent (N) nucleotides and the pairing of different - and -chains contribute to increase TCR diversity (2). Only one DNA polymerase, the nuclear enzyme TdT, catalyzes the addition of deoxynucleotide triphosphates onto the DNA 3' OH ends in a non-germline-encoded manner, during the joining phase of the rearrangement reactions (3, 4). In the absence of TdT activity, CDR3s were shown to be shorter and less diverse (3, 5, 6). We have recently shown that 90–95% of TCR diversity is due to TdT and no compensatory mechanism counterbalances the decrease in diversity in TdT-deficient mice (TdT°/°) (7). Despite this diminished TCR diversity, immune responses against viruses such as vesicular stomatitis virus and lymphocytic choriomeningitis virus (LCMV)3 and protein Ags are unimpaired and epitope immunodominance is likewise unaltered in TdT°/° animals (8). T lymphocytes from TdT+/+ and TdT°/° mice reacted with the same dominant epitopes of heat shock protein 65 from Mycobacterium tuberculosis and hen egg lysozyme (HEL) (8). When infected with the mouse pathogen LCMV or contaminated in a conventional colony by Sendai virus, TdT°/° mice recover from these infections. Altogether, these results indicate that TdT°/° animals are not immunodeficient.
T cell repertoire studies have previously shown that T cells specific for immunodominant (ID) or subdominant peptides express TCR with V-J and V-D-J rearrangements common to all mice or humans of the same MHC haplotype (9, 10, 11, 12, 13, 14). These rearrangements are referred to as public while private repertoires include those with V and V rearrangements different from one individual to the other (9). Depending on the antigenic peptide used, one or more public rearrangements could be identified. In BALB/c mice, the T cell response against the ID HEL103–117 peptide and the subdominant HEL7–31 epitope involve a single public rearrangement for each epitope (15). Moreover, in BALB/c mice infected with LCMV, Sourdive et al. (16) have shown that 70% of CD8+ T cells sorted with NP118–126 ID epitope/Ld tetramers expressed three different V. In a different antigenic model, Faure et al. (17) found three public V-J rearrangements specific for peptide 134–148 derived from the constant region of the Ig 
L chains. In L chain knockout (ko) mice (–/–) neonates born from +/– mothers, C-specific CD4+ T cells were tolerized by mother IgG. This state of tolerance was reversible and disappeared shortly after weaning, when the presentation of the C peptide stopped. Interestingly, the V-J public repertoires against the C peptide were re-expressed sequentially and the three canonical CDR3 sequences were detected at 52 wk of age in all animals, showing the high stability of public repertoire throughout life (15).
One may wonder what the driving forces are which lead to the emergence of public and private repertoires against a single epitope in primary immune responses. Two hypotheses have been put forward to explain the presence of public repertoires, i.e., higher avidity and/or higher precursor frequency as compared with private ones, no direct tests of these hypotheses have yet been performed in primary immune responses. Furthermore, it is not known whether public and private repertoire-bearing T lymphocytes fulfill different functions and/or have the same fate during secondary responses. However, Mikszta et al. (18) have shown that CD4+ T lymphocytes expressing dominant pigeon cytochrome c clonotypes localized in the germinal centers, 9 days following immunization, while T cells bearing subdominant TCR clonotypes do not migrate significantly in the same areas. Both types of T cells were nevertheless able to enter the pool of memory cells and contributed equally to the secondary response against pigeon cytochrome c.
The analyses of crystal structures of the TCR have shown that V or V chain forms three loops that interact with the peptide/MHC class Ia or class II molecules (reviewed in Refs.19 and 20). These loops correspond to the CDR1 and 2 which are encoded in the V and V genes while the CDR3 is produced by somatic recombination of V, (D), J segments. In crystal structures of most TCR-peptide-MHC class Ia complexes, CDR1 and CDR2 interact with the MHC helices whereas the highly diverse CDR3s contact the antigenic peptide. In a recent review of the crystal structure of 15 TCR-peptide/MHC complexes, Housset and Malissen (20) have summarized some of the general features found in these complexes. In all structures, the CDR1 and CDR2 loops always interact with the MHC2 helix even though these interactions are variable from one V chain to the other. A general mode of binding of the CDR1 and CDR2 to the peptide/MHC complexes is less apparent although CDR2 preferentially contacts the MHC1 helix. The recent structural studies of a public TCR complexed to an EBV antigenic peptide bound to HLA-B8 (21) show that amino acid residues highly selected for by the recombination of the V, (D), J segments and N-addition elements contribute to the fine specificity of this receptor. Additionally, they revealed a conformational change at the N-derived residue Pro93 in the CDR3 which was not observed in the structure of the TCR without its ligand (21, 22). Importantly, the N-region-encoded residue Gln98 in the CDR3 interacts with the HLA-B8 1 helix and two peptide residues (21).
In view of the major role played by TdT in generating CDR3 diversity, the question of whether or not public repertoires are selected for after TdT fine tuning of the TCRs seemed to us quite relevant. To test this hypothesis, we have compared the V and V public repertoires of TdT+/+ and TdT°/° mice immunized with CD4 and CD8 T cell epitopes. We found that the V, (D), J usage observed in public repertoire is strikingly conserved in TdT°/° mice and that T lymphocytes bearing TCR lacking N diversity can undergo affinity maturation. These findings are discussed in the context of the interactions between TCR and MHC peptide complexes.
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Materials and Methods |
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All mice used in this study were 6-wk-old TdT°/° (C57BL/6 background) (3) or C57BL/6 mice raised in specific pathogen-free conditions and obtained from the Pasteur Institute housing facilities and Charles River Laboratories, respectively.
Peptides
NP366–74 (ASNENMETM), E52–66 3Kp (ASFEAQKAKANKAVD), HBVc129–40 (PPAYRPPNAPIL) were produced by NEOSYSTEM. Their purity was tested by HPLC.
Immunizations and cell cultures
Mice were immunized in the hind footpads with 10 nmol of HBVc129–140 peptide or E52–66 3Kp in CFA. Nine days later, popliteal lymph nodes were collected, and lymph node cells (LNC) were cultured at 5 x 105 cells per well with different concentrations of the peptide and 5% CO2 for 4 days. All cultures were done in HL-1 medium (Cambrex) supplemented with glutamine (2 mM). The cultures were then pulsed with 1 µCi of [3H]thymidine (ICN Biomedicals) for the last 8 h of the 4-day culture.
For CD8+ T cell responses, mice were immunized in quadriceps muscles with 50 µg of DNA plasmid (pCI mammalian expression vector; Promega) encoding the nucleoprotein (NP) of influenza virus (strain A/PR8/34). Fourteen days later, splenocytes were collected, depleted of B220+ cells and cultured at 5 x 106 cells per well with 2.5 x 105 dendritic cells previously loaded with the ID epitope of the NP (NP366–74) and 5% CO2 for 5 days. Then, a cytotoxic assay was performed as described elsewhere (23).
Abs and sorting of cells
CD11c and B220 beads were from Miltenyi Biotec, FITC-CD4 and FITC-CD8 mAbs were from BD Pharmingen, Db-NP366–74 MHC class I molecule tetramers were produced as described elsewhere (24, 25) and I-Ab E52–66 3Kp MHC class II molecule tetramers were a gift from Drs. J. Kappler and P. Marack (Department of Immunology, Howard Hughes Medical Institute, Denver, CO) and were prepared as described (26). In both cases, biotinylated complexes were mixed with PE-labeled UltraAvidin (Leinco Technologies).
Dendritic cells were prepared from spleen of wild-type mice. Cells were treated with collagenase IV (Sigma-Aldrich) and DNase (Sigma-Aldrich) and stained at 4°C for 20 min using CD11c-MACS beads. The positive fraction was purified using AUTOMACS (Miltenyi Biotec). Splenocytes from mice were depleted of B220+ cells using beads and AUTOMACS. B220-negative splenocytes were incubated with indicated Abs at 4°C for 1 h, and washed. Cells were sorted on an Epics-Elite ESP (Beckman Coulter) at the Flow Cytometry Unit (Institut Jacques Monod, Paris, France). Cell purity after sorting was analyzed by flow cytometry and was above 96% in all samples.
RNA extraction and cDNA synthesis
Unfractionated or sorted T lymphocytes from TdT°/° and C57BL/6 mice were used for RNA preparation. Total RNA from splenocytes was extracted using an RNeasy minikit from Qiagen and reverse-transcribed into cDNA using oligo(dT) and SuperScript II (Invitrogen Life Technologies).
Immunoscope analyses
PCR were conducted in 50 µl on 1/50 of the cDNA with 2 U of Taq polymerase (Promega) in the supplier’s buffer. cDNA was amplified using V- or V-specific sense primers and antisense primers hybridizing in C or C segments. Amplified products were then used as template for an elongation reaction with fluorescent-tagged oligonucleotides (run-off reactions) as described elsewhere (27).
Cloning and sequencing of TCRBV and TCRAV rearrangements
The cloning and sequencing method has been described elsewhere (28). In more details, we used a TOPO Blunt cloning kit (Invitrogen Life Technologies). A PCR amplification was performed on cloned bacteria and was followed by a second step of elongation using an ABI PRISM Big Dye Terminator kit (Applied Biosystems). Sequencing products were then read on 96 capillaries (3700 DNA Analyzer; Applied Biosystems).
Tetramer staining and kinetics of dissociation
This was done as described by Savage et al. (29). Briefly, T splenocytes were stained at 4°C for 1 h with Db-NP366–74 tetramer and mAb (CD8-FITC). Cells were washed and the nonlabeled anti-Db mAb KH95 was added. Tetramer staining was evaluated at different times between 0 and 120 min after KH95 addition. The normalized fluorescence (f) corresponds to the total fluorescence at a given time (Fx) divided by the total fluorescence at the initial time point (F0). The total fluorescence corresponds to the sum of the fluorescence intensities of CD8+Db-NP366–74 tetramer-positive cells divided by the total number of CD8+ cells. Then, the ratio ln (Fx/F0) is plotted vs time. For each time interval of the plots, the mean slope of an interval is estimated as the equivalent of ln (fa/fb)/t, where fa is the normalized fluorescence at the start of the interval, fb the one at the end of the interval, and t is the length of the interval (hour).
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Results |
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Two strategies were used to characterize in C57BL/6 mice the repertoire of T lymphocytes specific for the peptide 129–140 of the core protein of the hepatitis virus (HBVc): 1) CD4+ T cell hybridomas specific for this peptide were generated and their TCR -chains sequenced. Two of 10 CD4+ T cell hybridomas reacting with HBVc129–140 used the rearrangement V11-J2.7 with a CDR3 of 7 aa. The sequences of their CDR3 are as follows: SLQIYEQ and SLGGDEQ (Table I). 2) The Immunoscope method was used to identify the T cell repertoires of C57BL/6 and TdT°/° mice, in response to the HBVc peptide 129–140. Briefly, the RNA extracted from in vitro HBVc peptide 129–140-stimulated LNC were reverse transcribed into cDNA, and aliquots were amplified by PCR with 24 V- and C-specific primers or the 21 V- and C-specific primers. The products from each PCR were then divided into aliquots and a run-off reaction was performed with the dye-labeled oligonucleotides specific for C or with C. The fluorescent runoff products were analyzed in an automated DNA sequencer. In C and C runoff products, we observed public expansions corresponding to peaks with CDR3 of various amino acid lengths. Every V-C PCR product corresponding to public expansions was divided into 12 aliquots that were hybridized with a fluorescein-labeled oligonucleotide specific for each of the 12 J segments. A runoff reaction was performed and the sizes of the fluorescent runoff products were analyzed.
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11-J2.7 rearrangements corresponding to two peaks with a CDR3 of 7 and 9 aa in C57BL/6 mice and a single peak with a CDR3 of 7 aa in TdT°/° animals (Fig. 1B, upper panels). Those peaks were found in all C57BL/6 mice and in four TdT°/° animals of five. In contrast, bell-shaped curves, characteristic of naive polyclonal repertoires, were found in the purified protein derivative controls (data not shown). Furthermore, we found a public expansion for V12 with a CDR3 of 8 aa in both strains of mice (Fig. 1B, lower panels).
The PCR products were cloned and sequenced. As shown in Table I (in bold letters), a CDR3 of 7 aa corresponding to the SLQAYEQ sequence was found in the V11-J2.7 rearrangement from 6 mice of 10. It is worth noticing that this sequence is generated without N diversity in 4 TdT°/° animals while it is produced with N addition in 2 C57BL/6 mice. Two CDR3 of 7 aa, coded by different nucleotidic sequences, share the same amino acid sequence(SLGGYEQ). Finally, the CDR3 sequences of two different HBVc129–140-specific T cell hybridomas are closely similar to most of the CDR3 sequences observed in different animals. The recurrent CDR3s of 9 aa are found in C57BL/6 mice only. A comparison of the 9 aa CDR3 sequences of 5 C57BL/6 shows that the CDR3 sequences of mice nos. 2, 4, and 5 reveal a consensus sequence SLXGSSYEQ; in addition, a SLXGGXYEQ consensus can be found in CDR3 sequences of animal nos. 3, 4, and 5. In C57BL/6 nos. 4 and 5, two different nucleotidic sequences generate the same CDR3, i.e., SLTGGPYEQ (in italic in Table I).
In Table II are shown the CDR3 sequences. Interestingly, the V12-J27 rearrangement is used in all C57BL/6 and four TdT°/° mice. It generates CDR3 with the amino acid sequence SDTNTGKL in TdT°/° animals while in C57BL/6 the CDR3 sequences are more diverse (in bold in Table II). However, the aspartate residue (D) in the SDTNTGKL sequence is replaced by a glutamate residue (E) (conservative mutation) in three different C57BL/6 or by a glycin residue (G) in mouse no. 2. The sequence TNTNTGKL produced by the rearrangement V12-J27 is also found in two different C57BL/6, but not TdT°/°, animals.
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-J and V-J usage in C57BL/6 and TdT°/° is highly conserved in T cell responses against the HBVc peptide 129–140. Furthermore, while the 9 aa CDR3 is found in C57BL/6 mice only, the short CDR3 sequences are homologous in both strains of mice. CDR3 sequences are similar because the sequence SDTNTGKL is found in four TdT°/° while sequences SETNTGKL or SGTNTGKL are present in three and one C57BL/6, respectively.
Repertoire analysis of CD4+ cells reactive against the E52–66 3K peptide in C57BL/6 and TdT°/° mice
The comparison between C57BL/6 and TdT°/° mice in terms of rearrangements used by peptide-specific CD4+ T cells was extended to another antigenic system, namely the E52–66 3Kp which induces strong proliferative responses in C57BL/6 mice. Moreover, the availability of E52–66 3Kp/I-Ab tetramers allows for the isolation of peptide-specific T cells (26).
Mice were immunized and 9 days later, LNC were restimulated in vitro with increasing concentrations of E52–66 3Kp (Fig. 2A). We sorted out by flow cytometry the specific CD4+ T cells from LNC with E52–66 3Kp/I-Ab tetramers. Immunoscope analyses revealed an amplification of the rearrangement V7-C of 8 aa (Fig. 2B, lower panels). For the V, we observed that, in all animals, the V6-J2.7 segment was amplified. The PCR products were cloned and sequenced. A CDR3 of 6 aa with the sequence SMDYEQ is found in all TdT°/° animals while CDR3 of 6 and 9 aa are identified in C57BL/6 mice (Table III). For the CDR3 of 6 aa the consensus sequence found in C57BL/6 is SXDYEQ and the SMDYEQ sequence is found in C57BL/6 no. 2 only (in bold in Table III). For the CDR3 of 9 aa, the consensus sequence is SXXDWGYEQ and the sequence SMGDWGYEQ is found in three C57BL/6.
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sequences in response to E52–66 3Kp. All C57BL/6 mice use the V7-J13 rearrangement generating the SANSGTYQ sequence (in bold in Table IV). In two TdT°/° animals, the same rearrangement and CDR3 sequence are found. Surprisingly, two TdT°/° mice use a different rearrangement (V7-J26) even though the SANSGTYQ sequence does not contain N diversity. The J26 segment is also found in C57BL/6 mouse no. 2 leading to the CDR3 sequence SENYAQGL while in TdT°/° using the same J segment the CDR3 sequence is SDNYAQGL (in italic in Table IV). It is striking that in the rearrangement containing N diversity the aspartate residue (D) found in the CDR3 of the TdT°/° animals is replaced by a glutamate residue (E). As observed for the HBVc peptide 129–140, the V-J and V-J usage in T cell responses against the E52–66 3Kp is highly conserved and the CDR3 sequences are homologous in both strains of mice. In addition, we found, by quantitative PCR on tetramer-positive T lymphocytes, that the percentages of E52–66 3Kp-specific V7 segment were similar in both strains of mice i.e., 16.3 ± 2.4% in C57BL/6 vs 18.8 ± 5.5% in TdT°/° (four mice for each group).
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The repertoire of CD8+ T cells generated in response to the ID peptide of the NP of influenza virus (NP366–74) was next identified. This antigenic model presents the advantage that peptide-specific T lymphocytes can be purified using NP366–74/H-2 Db tetramers.
Mice were immunized with 50 µg of plasmid encoding the NP of influenza virus. Fourteen days later, splenocytes were cultured for 5 days in the presence of NP366–74 peptide-pulsed dendritic cells and their cytotoxic activity was tested (Fig. 3). T cells from both C57BL/6 and TdT°/° mice are efficient in killing peptide-pulsed target cells and no significant statistical difference between the two mouse strains was observed. NP366–74 peptide-specific cells were then sorted out from spleens of immunized mice, following staining with the NP366–74/H-2 Db tetramers. V and V T cell repertoire analyses are shown in Fig. 3B. For both strains of mice, an oligoclonal expansion of the V8.3-J2.2 of 9 aa length is observed (Fig. 3B, upper panels) while oligoclonal expansions of the V16 with a CDR3 length of 10 and 8 aa are found in C57BL/6 and TdT°/°, respectively (Fig. 3B, lower panels). In Tables V and VI are shown the CDR3 and sequences. All TdT°/° mice use the CDR3 sequence SGGANTGQL, while four C57BL/6 mice use preferentially the CDR3 sequence SGGSNTGQL and one animal the sequence SGGGNTGQL (Table V, in bold). Overall, the consensus sequence for both strains of mice is SGGXNTGQL. It is worth noticing that in TdT°/° no. 3, the sequence SGGANTGQL is mainly generated by germline nucleotidic sequence while, in 4% of the bacterial clones sequenced, it is produced with a single P addition.
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16-J15, the same CDR3 RDQGGRAL is found with the highest frequency in the bacterial clones sequenced (Table VI, in bold). Another sequence RANSGTYQ is found in TdT°/° mice nos. 1 and 4. This sequence is generated by the V16-J13 rearrangement. However, all wild-type mice use the V16-J42 with a longer CDR3 (10 aa). The consensus sequence is RXSGGSNAKL. It is interesting to note that beside this rearrangement, the V16-J15 combination found in all TdT°/° mice is also used by three C57BL/6 animals of four. Interestingly, in C57BL/6 mouse no. 2 the sequence RDQGGRAL is observed as in TdT°/° mice while for the other two mice the V16-J15 rearrangements are longer by two residues.
From these results, we conclude that two V-J public rearrangements (V16-J42 and V16-J15) are observed in C57BL/6 animals while a single public rearrangement (V16-J15) is used in TdT°/° mice. As far as the V chain is concerned, there is a conservation of the V-J combination and the CDR3 from C57BL/6 differs from those of TdT°/° by A vs G or S (Table V) only. As in the previous paragraph, we have assessed by quantitative PCR on isolated tetramer-positive T lymphocytes the percentages of NP366–74-specific V16 usage. The values obtained were similar and averaged 48.2 ± 10.6% in C57BL/6 and 43.6 ± 7.9% in TdT°/° (four mice for each group).
Avidity and dissociation kinetics of NP-peptide/H-2Db tetramers bound to specific CD8+ splenocytes
Several groups have demonstrated that secondary T cell responses use a more focused repertoire than primary responses (29, 30, 31), a consequence of the preferential selection and expansion of T lymphocytes bearing TCR with the highest affinity. This raises the possibility that the decrease in TdT°/° T cell repertoire diversity may lead to an absence of affinity maturation process. To verify this hypothesis, we compared the dissociation kinetics of NP-peptide/H-2 Db tetramers bound to CD8+ splenocytes from primary and secondary responses of C57BL/6 and TdT°/° mice as described by Savage et al. (29). In Fig. 4A the schedule of immunization with the pCI-NP plasmid encoding the full-length NP of influenza virus and the pCI plasmid as a control is shown. Briefly, mice were immunized, 14 days later the splenocytes from primary responses were collected and analyzed. For secondary responses, immunized mice were boosted 2 mo after the first immunization and splenocytes were collected 1 wk after. For primary and secondary responses, the splenocytes were incubated, at 4°C for 1 h, with NP-peptide/H-2 Db-PE-streptavidin tetramers and an anti-CD8 mAb. An aliquot was sampled out and corresponded to T0 h. An unlabeled anti-H-2 Db mAb was added to the remaining cells at 4°C and aliquots were collected at different times. The anti-H-2 Db was used in excess as compared with H-2 Db molecules borne by T cells to inhibit binding of dissociated tetramers during our analysis. In Fig. 4B is shown a typical experiment, 2.65% tetramer-positive CD8+ T lymphocytes are observed whereas only 0.09% are observed in a control mouse. The percentage of tetramer-positive CD8+ T cells in C57BL/6 and TdT°/° animals during the primary and secondary responses is presented in Fig. 4C. In both mouse strains, tetramer-positive CD8+ cells represent between 2 and 3% of CD8+ cells and this number is not significantly different in primary or secondary responses. The mean fluorescence intensities observed in primary and secondary responses were similar in C57BL/6 and TdT°/° mice (Fig. 4C). Tetramer staining decay kinetics show significant differences between primary and secondary responses in C57BL/6 as well as in TdT°/° mice (Fig. 5, A and B). This may indicate that, in secondary responses, T cell mean avidity is increased as compared with primary responses. Interestingly, comparison of the dissociation rate (koff) at early time points (0–20 min) shows a faster koff in C57BL/6 primary responses while at other time intervals the dissociation rate is almost identical for primary and secondary responses. In TdT°/°, faster koff are observed in primary responses between 0 and 40 min, at later times the koff in primary and secondary responses are not statistically different. As suggested by Savage et al. (29), these results may be indicative of the presence in primary responses of subsets of T lymphocytes bearing TCR with the highest koff for their ligands. In secondary responses, these T cells are outcompeted by T lymphocytes bearing TCR with lower koff for their ligands.
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Comparison of the number of N nucleotides present in the CDR3 of public and private rearrangements
Several non-mutually exclusive explanations underlie the extraordinary stability over time (15) and the favored selection of public repertoires: 1) public repertoires are generated more easily than private ones by the recombination machinery; 2) public repertoires which contain few N nucleotides are more efficiently selected for by MHC molecules during thymic selection; and 3) private repertoires bearing more N nucleotides in their CDR3 are selected by peptide/MHC complexes but they have a better fit for the aa residue side chains of the self-peptide.
In Table VII are shown statistical analyses of the numbers of N nucleotides in the CDR3 of published public and private repertoires. We found a statistically significant difference between the number of N nucleotides contained in CDR3 of public vs private rearrangements (1.64 vs 3.09 N nucleotides, p < 0.001). The same analysis for the CDR3 could not be performed because the numbers of public vs private V repertoires available are insufficient.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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We report in this work that public V-J and V-D-J usage against three different epitopes presented by MHC class Ia or II molecules are strikingly conserved in TdT°/° mice. Furthermore, the C57BL/6 and TdT°/° CDR3 sequences are highly homologous. In addition, we found, by quantitative PCR on tetramer-positive T lymphocytes, that the percentages of public V segment usage for each peptide were similar in both strains of mice.
TdT°/° T cells gave good proliferative recall responses to the MHC class II-restricted epitopes, HBVc129–140 and E52–66 3Kp, albeit significantly reduced compared with those of TdT+ cells (Fig. 1A). Against HBVc129–140, V12-J27 and V11-J2.7 rearrangements are used in all TdT°/° and TdT+/+ mice. For the V-J rearrangement, the public CDR3 sequences are closely similar, the D residue found in position 2 in the CDR3 of TdT°/° animals is replaced by G or E in C57BL/6 mice (Table II) and all CDR3 have the same length of 8 aa. Two CDR3 lengths are selected in C57BL/6 animals while in TdT°/° mice only the shorter CDR3 is found (Table I and Fig. 1B). This shortening of CDR3 is a characteristic feature of TdT°/° V chains, as previously reported for double-positive CD3low thymocytes and mature T lymphocytes (7, 32).
Shorter CDR3 (6 aa instead of 9 aa) is also observed in the V6-J2.7 rearrangement selected in TdT°/° T cells specific for the peptide E52–66 3Kp. In most CDR3, M is found in position 2 in the long and short CDR3. Interestingly, in 18 CDR3 of 21 (Table III) there is a D residue which may be strongly selected for by the E52–66 3Kp, reminiscent of the selection of positively charged residues (K or R) in the CDR3 of the MCC-specific TCR when the T residue from the antigenic MCC peptide is substituted by a glutamate (33). The crystallographic structure of the E54–66 3Kp peptide bound to IAb showed that five amino acids at p1, p2, p3, p5, and p8 were TCR contact residues; in addition, mutation of one of the three K completely abolished recognition by two specific T cell hybridomas (34). Thus, it is conceivable that the D residues found in the CDR3 form a salt bridge with one of the K residues of the antigenic peptide E52–66 3Kp. Concerning the V7-J13 used in response to E52–663Kp, the CDR3 sequence is identical in all TdT+ and TdT°/° animals because it is generated by germline rearrangement of V7 and J13 segments. Interestingly, the CDR3 sequences SENYAQGL and SDNYAQGL are found in one C57BL/6 and two TdT°/° animals, respectively. It is possible that the presence of a glutamate residue in position 2 of SENYAQGL is selected for, because it is produced by N addition.
TdT°/° and TdT+/+ CTLs generated in response to the MHC class I-restricted NP of influenza virus efficiently killed NP peptide-pulsed target cells. The V public repertoire was homologous because the CDR3 sequences differed by one residue: A replaced S or G (Table V). The V public repertoire uses the same V-J rearrangement in both mouse strains, however, a CDR3 of 10 is selected in TdT+/+ while a shorter CDR3 is found in TdT°/° mice. The consensus sequences were identified as RXSGGNAKL and as RDQGGRAL in C57BL/6 and TdT°/° mice, respectively. Interestingly, the public V repertoire identified by Gavin and Bevan (6) in their collection of NP peptide-specific clones includes sequences SGGS/GNTGL (three clones of eight for C57BL/6) and SGGANTGQL (six clones of eight in TdT°/°) (6). However, the public V repertoires could not be predicted from the CTL clones. The Immunoscope approach offers the advantage of allowing an easy identification of the V and V repertoires against different epitopes (9, 10, 15, 17, 35) but cannot determine with certainty which V and V are associated in T cell clones. The association of the CDR3 (RDGGRAL) and the CDR3 (SGGANTGQL), corresponding to the recurrent rearrangements in TdT°/° mice, was found in one CTL clone described by Gavin and Bevan (6). Interestingly, this clone is the most cross-reactive and detects 47 HPLC fractions from the H-2Db-restricted peptide library (6).
Among the CTL clones produced by Gavin and Bevan (6), 50% of those derived from C57BL/6 use different V-J rearrangements and various V-J combinations. In contrast, most of the TdT°/° clones bear the same V chains (six of eight). This decrease in diversity of the T cell repertoire in TdT°/° mice could lead to a lack of avidity maturation. The dissociation kinetics of NP-peptide/H-2 Db tetramers bound to CD8+ splenocytes from primary and secondary responses of C57BL/6 and TdT°/° mice (29) showed that avidity maturation occurs in both mouse strains but the large variations in staining decay, found in primary responses to MCC/I-Ek complexes in different mice, were not observed (29). In our model Ag, a statistically significant difference in avidity was observed between primary and secondary responses in both C57BL/6 and TdT°/° mice. The smaller individual variations observed may be due to the highly frequent T cell clones sharing TCRs of homogenous avidity for the NP peptide. Another possibility is that the recognition of this peptide/MHC complex by CTL is highly dependent on the CD8 molecule blurring differences in TCR affinity.
The recognition by individual TdT°/° CTL clones of a large panel of different MHC class I/antigenic peptide complexes has been reported by Gavin and Bevan (6) and these latter authors have suggested that TCR bearing no N additions interact predominantly with the MHC class I helices and less so with the peptides. This hypothesis is in agreement with the finding that a greater number of double-positive thymocytes undergoes positive selection than in TdT+/+ mice (32).
Taking into consideration all these observations, we suggest that public repertoires bear the imprint of MHC molecules while private ones are more influenced or are selected by MHC bound self-peptides. Thus, three levels of complexity exist in the T cell repertoire: 1) the repertoire of neonates without N diversity and which interacts strongly with MHC molecules is thereby highly peptide cross-reactive; 2) the public repertoires which use the same V-J rearrangements as the neonatal ones and which include a moderate amount of N nucleotides and recognize a more restricted panel of antigenic peptides while keeping a good interaction with MHC molecules; and 3) the private repertoires which use distinct V-J combinations for each individual mouse and which contain a still larger proportion of N nucleotides (Table VII) in their CDR3 and interact in a highly specific way with the antigenic peptide and more weakly with the helices of MHC molecules.
Several sets of experimental data suggest that CDR1 and CDR2/MHC interactions may play a predominant role in the selection of public repertoires. Single-site alanine mutagenesis of the 2C TCR has shown that CDR1 and CDR2 of V and V chains contribute more to the binding energy with the MHC class I residues than CDR3s (36). Moreover, they estimated that two-thirds of the total energy is due to TCR residues interacting with MHC helices. Studies of the interaction of the 2B4 TCR with MHC class II molecules complexed to a MCC peptide led to the two-step model of TCR recognition in which TCR/MHC interactions are required for association of the complex, while TCR/peptide contacts are involved mainly in stabilizing the complex (37). In the crystal structure of a public TCR bound to its cognate MHC-peptide, CDR2 contains five residues that interact with the HLA-B8 1 helix and are found only in 2 of 54 human V genes (V7-8 and V7-9). Finally, CDR1 and CDR2 residues from the V3.1 and V3.2 chains select preferentially CD4+ or CD8+ cells, presumably by stimulating interactions with either MHC class I or class II molecules (38). Overall, CDR1 and CDR2 interactions with MHC molecules are probably involved in positive selection in the thymus and are implicated in the generation of public repertoires, while CDR3 interactions may either favor negative selection or, in the case of long CDR3s, hinder positive selection.
Further studies in support of our hypothesis would necessitate the production of MHC class I molecules containing single mutations of the helix amino acid residues with side chains interacting with the V or V chains of the TCR to assess the effect of these mutations on the public repertoires in response to various epitopes.
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Footnotes |
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1 This work is funded by INSERM, CNRS, University of Orsay and the Pasteur Institute. N.F. is supported by l’Association de Recherche contre le Cancer and by the Ministère de la Recherche. J.M.K. is supported by a grant from l’Association de Recherche contre le Cancer. 
2 Address correspondence and reprint requests to Dr. Jean M. Kanellopoulos, Laboratoire d’Activation Cellulaire et Transduction des Signaux, Institut de Biochimie et de Biophysique Moléculaire et Cellulaire, UMR 8619 CNRS, B
timent 430, Université Paris Sud, 91405 Orsay Cedex, France. E-mail address: Jean.Kanellopoulos{at}ibbmc.u-psud.fr
3 Abbreviations used in this paper: LCMV, lymphocytic choriomeningitis virus; HEL, hen egg lysozyme; ID, immunodominant; LNC, lymph node cell; NP, nucleoprotein; HBVc, core protein of the hepatitis virus; MCC, moth cytochrome c; koff, dissociation rate.
Received for publication July 9, 2004. Accepted for publication October 26, 2004.
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