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The Journal of Immunology, 2004, 173: 4197-4206.
Copyright © 2004 by The American Association of Immunologists

IL-8 Is a Key Chemokine Regulating Neutrophil Recruitment in a New Mouse Model of Shigella-Induced Colitis

Monique Singer1 and Philippe J. Sansonetti2

Unité de Pathogénie Microbienne Moléculaire, Institut National de la Santé et de la Recherche Médicale U389, Institut Pasteur, Paris, France


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
The lack of a mouse model of acute rectocolitis mimicking human bacillary dysentery in the presence of invasive Shigella is a major handicap to study the pathogenesis of the disease and to develop a Shigella vaccine. The inability of the mouse intestinal mucosa to elicit an inflammatory infiltrate composed primarily of polymorphonuclear leukocytes (PMN) may be due to a defect in epithelial invasion, in the sensing of invading bacteria, or in the effector mechanisms that recruit the PMN infiltrate. We demonstrate that the BALB/cJ mouse colonic epithelium not only can be invaded by Shigella, but also elicits an inflammatory infiltrate that, however, lacks PMN. This observation points to a major defect of mice in effector mechanisms, particularly the lack of expression of the CXC chemokine, IL-8. Indeed, this work demonstrates that the delivery of recombinant human IL-8, together with Shigella infection of the colonic epithelial surface, causes an acute colitis characterized by a strong PMN infiltrate that, by all criteria, including transcription profiles of key mediators of the innate/inflammatory response and histopathological lesions, mimics bacillary dysentery. This is a major step forward in the development of a murine model of bacillary dysentery.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
The Gram-negative invasive bacterial pathogen Shigella flexneri is responsible, in humans, for an acute rectocolitis characterized by a bloody diarrhea, reflecting severe mucosal destruction leading to dysentery (1). This disease occurs mostly in the low hygiene areas of the developing world, infants and young children being the primary victims. The lack of a mouse model of intestinal, or better, colonic infection by Shigella that would mimic the acute invasive inflammatory process leading to epithelial and mucosal destruction is a serious handicap to Shigella research because it affects basic studies in molecular pathogenesis, and studies aimed at understanding the mechanisms of local innate and adaptative immune response. The lack of a murine model is also a handicap in Shigella vaccine development because there is currently no simple and reliable model to screen both live attenuated oral and subunit parenteral vaccine candidates at the preclinical stage. Interestingly, resistance to Shigella is not inherent to the mouse species. Some organs are sensitive such as the bronchoalveolar tree (2), and recently a model of acute invasive jejunitis has been developed in new born mice <5 days old, following intragastric inoculation (3). In adult mice, a factor, or a combination of factors, may thus be missing in the course of the interaction between Shigella and the tight barrier of the colonic epithelium, probably resulting in the refractory state. It may reflect a mouse-specific defect in a receptor, or in an apical signaling system that impairs epithelial colonization and invasion, compared with the human colon. Alternatively—or in addition—it may reflect a defect in sensing or adequately responding to Shigella in the course of the invasive process, by not secreting the relevant proinflammatory cytokines and chemokines that cause the acute PMN infiltrate characteristic of shigellosis. Defects in IL-10 or IL-2 production, or in TCR expression in knockout mice (4), allow development of an enterocolitis characterized by an acute neutrophilic and macrophagic infiltrate, indicating that there is no intrinsic defect of the murine intestinal mucosa to develop a strong polymorphonuclear leukocyte (PMN)3 infiltrate (5, 6). Previous studies suggest a dominant role of IL-8 in stimulating neutrophilic inflammation. Indeed, in a rabbit model of Shigella-induced ileitis (7), neutralization of IL-8 by an anti-IL-8 mAb, almost completely abrogates the pathological process. In the guinea pig, a species that, like the rabbit, expresses IL-8, intragastric inoculation of the invasive M90T strain induces an ileitis with some PMN recruitment (8). In addition, in human epithelial cells, invasive Shigella induce the activation of the NF-{kappa}B system through Nod 1 (9), a peptidoglycan sensor (10, 11), thereby promoting massive expression of IL-8 (12). The predominance of IL-8 is confirmed by global transcriptional profiling of Shigella-infected human colonic Caco-2 cells, showing that the amount of IL-8 mRNAs is increased by 300-fold following 2 h of infection by an invasive strain, compared with infection with a noninvasive strain, the IL-8 gene being indeed, by far, the most transcribed (13).

In the mouse, the genes encoding IL-8 and its receptor CXCR1 have been deleted, probably when a gene duplication event occurred in a common ancestor of rat and mouse, because these genes are lacking in the muroid lineage (14). However, the murine MIP-2, or the murine keratinocyte-derived protein chemokine KC seem to be the functional homologues of IL-8. They share the extracellular loop reactive+ motif (Glu-Leu-Arg) for the chemoattractant properties of IL-8 family members (15, 16). They also share LPS inducibility (14). Despite the lack of a gene coding for IL-8, the mouse species possesses a receptor homologous to human CXCR2 that is able to mediate neutrophil chemotaxis in response to human IL-8, in addition to MIP-2 and KC (16, 17). Therefore, we postulated that the absence of IL-8 production in mice may prevent Shigella-induced colitis. This hypothesis has been tested by adding exogenous recombinant human IL-8 (rhIL-8) in the course of an experimental infection by Shigella, and by comparing its effects to the addition of other neutrophil chemoattractants, such as the murine CXC chemokines recombinant murine (rm)MIP-2 and rmKC (17, 18).

Thus, we present in this study a new adult model of colonic infection by Shigella in which a double intraluminal addition of rhIL-8, concomitant to invasive S. flexneri, induces mucosal lesions that, by all standards, appear similar to the human disease. This study confirms that the lack of IL-8 expression is either the major or one of the major factors explaining the refractory state of mice to develop acute invasive colitis in the presence of Shigella.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Animals, inoculation, and materials

Male BALB/c mice (Centre d’Elevage R. Janvier, France), aged 5–6 wk, were fasted for 24 h with water ad libitum. The next day, they were anesthetized with xylazine 12% (20 mg/kg) ketamine 500 (45 mg/kg) (both from Sigma-Aldrich, St. Louis, MO), and groups of five mice were challenged by intracolonic (i.c.) route, inserting a cannula of 40 mm in the rectum, up to the left angle of the colon and instillating either saline (endotoxin-free 0.9% NaCl), or rhIL-8 (AbCys, Paris, France), or rmKC or rmMIP-2 (both from AbCys), or a suspension of Shigella. rhIL-8 was used at doses of 1, 2, and 4 µg, then at the fixed dose of 2 µg, as well as for rmMIP-2 and rmKC, and adjusted to 50 µl in saline (19, 20). The i.c. route was chosen to overcome the drawbacks of the oral route (6, 21), and to deliver a calibrated dose of Shigella directly in contact with the colonic epithelium. Two stains of Shigella were used: either the noninvasive mutant mxiD that has lost the capacity to express the type III secretory apparatus that allows Shigella to deliver its effector proteins of invasion to the intestinal epithelium (22), or the invasive strain M90T (serotype 5) containing the 213-kb virulence plasmid that encodes the invasive phenotype (23). The inoculum of bacteria was obtained by overnight culture, and an aliquot was grown in 400 ml of Tripticase Soy Broth BBL medium (BD Biosciences, Le pont de Claix, France), up to midexponential growth phase (OD600 = 0.8). Bacteria were then concentrated by centrifugation, and kept at 37°C until i.c. instillation (24), and their number was determined by OD and by plating dilutions on Tripticase Soy Broth BBL agar, to perform inoculums at 109 up to 1011 CFU/ml. OD = 1 corresponded to 5.108 CFU, with a SD of 3%. Groups of five mice were inoculated once, either with 0.1 ml of the culture medium as control, or with 0.1 ml of a suspension of 107 up to 1011 CFU/ml invasive Shigella, or of the noninvasive strain mxiD. Following the selection of inocula of 109 and 1010 CFU/mouse, experiments were conducted with or without rhIL-8 instilled together with strain M90T or control mxiD. Similar experiments were performed with rmKC or rmMIP-2 (17, 18). A second intraluminal administration of rhIL-8 (or rmKC, or rmMIP-2), concomitant to inoculation of S. flexneri, was also performed on groups of five mice, 18 h following the first infection (25). Animals were then sacrificed at 24 h and their colons were collected. The kinetics of Shigella infection of the colon were performed using an inoculum of 109 CFU/mouse, by collecting samples of colon at 1, 3, 6, and 24 h following the initial infection. The whole colon was observed directly for the presence of blood, then its length was measured, and a first portion (5 mm) was cut at 40 mm from the anal orifice, a distance corresponding to the left angle, opened longitudinally, and observed to provide semiquantitative evaluation of the lesions expressed as scores; the following 5 mm of the descending colon were also retained for histology and scores after paraffin inclusion, and finally, another aliquot was kept for myeloperoxidase (MPO) and RT-PCR.

Evaluation of the lesions by macroscopic and histopathologic examination: Wallace and Ameho scores

The macroscopic lesions of the colon considered encompassed thickness and length of the colon, presence of blood, and edema. Stereomicroscopic examination of fresh colon longitudinally opened confirmed the previous observations and allowed an immediate evaluation of scores according to the Wallace criteria (26), based on the following data: hyperemia, edema, increase in thickness, number, and size of ulcerations, and presence of inflammation or purulent material.

Colons were dissected and prepared for histology: after washing in saline, they were inflated with 50% OCT (Sakura Finetck, Torrance, CA), and frozen, then sectioned in 5-µm slices to perform immunocytochemistry (ICC). For paraffin inclusion, colons were soaked in 10% formaldehyde in PBS overnight at 4°C, processed in paraffin wax, then positioned for longitudinal or ring section. Five-micrometer sections were stained with H&E, or Diff Quick (Baxter Merz & Dade, Düdingen, Switzerland), for identification of recruited cells in the colon.

Histopathological evaluation of colonic sections encompassed the degree of erosion of the epithelium alterations, of crypts, nature of the inflammatory infiltrate, and state of the lamina propria. This allowed determination of a score according to the gradation of Ameho (27).

Quantification of tissue MPO by Western blot

An aliquot of colon was homogenized with an Ultraturrax (T25 Janke and Kunkel; IKAR-Labortechnik, Staufen, Germany) for 1 min at 4°C in cold PBS containing Nonidet, and antiproteases (both from Sigma-Aldrich). After centrifugation, MPO was determined by Western blot (5, 28). The protein content was measured by OD at 205, 235, and 280 nm for each sample, then 50–200 µg of each was analyzed by SDS-PAGE (50 µg without precipitation, or 200 µg following ethanol precipitation). The transfer was performed to PVDF and blotting was performed overnight at 4°C, first with a goat anti-MPO (C-16) polyclonal serum (Santa Cruz Biotechnology, Santa Cruz, CA), then with a second polyclonal anti-goat HRP-conjugated serum (Sigma-Aldrich). Revealation was done by ECL-Plus (Amersham Bioscience, Buckinghamshire, U.K.) allowing quantification of chemiluminescence of the 70-kDa band using a Storm apparatus, and the ImageQuant program (Molecular Dynamics, Sunnyvale, CA).

Detection of S. flexneri invasion by ICC

Amplified ICC using first a biotin-conjugated Ab specific for LPS of S. flexneri (which recognizes invasive and noninvasive strains, a kind gift of Dr. A. Phalipon, Institut Pasteur, Paris, France), then a second streptavidin-peroxidase-conjugated Ab (DakoCytomation, Carpinteria, CA) was performed, the latter being revealed by 3-amino-9-ethylcarbazol (Sigma-Aldrich), as described (28, 29).

Cytokine and chemokine transcriptional profiling by quantitative RT-PCR

Portions of colons were isolated and thoroughly washed with saline. Dispersion was performed with an Ultraturrax (T25 Janke and Kunkel, IKAR-Labortechnik) for 40 s in the RNA Total Lysis buffer from the RNeasy Mini kit (Quiagen, Hilden, Germany) used for RNAs extraction. RT-PCR was performed for colon, using specific primers for TNF-{alpha}, 5'-ACT GAA CTT CGG GGT GAT CGG TCC; reverse, 5'-GTG GGT GAG GAG CAC GTA GTC G; IL-1{beta}, 5'-TTg ACg gAC CCC AAA AgA Tg; reverse, 5'-AgA Agg TgC TCA TgT CCT CAT; IL-18, 5'-ACT gTA CAA CCg CAg TAA TAC gg; reverse, 5'-gAg TgA ACA TTA CAg ATT TAT CCC; then those for IFN-{gamma} (Th-1), IL-4 (30); then MIP-2, 5'-CAg AAT TCA CTT CAg CCT AgC gCC AT; reverse, 5'-gCT CTA gAg TCA gTT AgC CTT gCC TTT g; KC, 5'-CAG CCA CCC GCT CGC TTC TC; reverse, 5'-TCA AGG CAA GCC TCG CGA CCA T; and {beta}-actin as a control, as described (28, 29, 30). Standards, when available, were prepared as described (29, 30, 31, 32). The copy number was calculated according to the OD, then the purified DNA was serially diluted to obtain the appropriate standard containing 0–1,000,000 of copies. The copy number of the sample was calculated relatively to the standard, after PCR amplification on the LightCycler System (Roche Molecular Biochemicals, Mannheim, Germany). When standards were not available, quantification was achieved using a Storm and ImageQuant (Molecular Dynamics), as described (28). Results were presented as a ratio of cytokine:{beta}-actin copies.

Statistical analysis

All results are presented as means ± SD. Levels of significance were calculated using one-way ANOVA, followed by Scheffe’s test, using the SPSS 6.1 software (SPSS, Chicago, IL) (*, significance between data with a threshold of p < 0.05).


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
In this work, we first established the characteristics of the infection process induced by the administration of invasive S. flexneri alone, in the left angle of the colon, compared with a noninvasive mutant. We observed limited inflammation and the lack of a PMN infiltrate, confirming the intestinal "refractory state" of mice to Shigella, in contrast with the capacity of humans to develop dysentery. Through careful evaluation of the observed lesions, we established a profile allowing us to draw conclusions about the possible defects underlying this refractory state. We tested these hypotheses by first addressing the lack of IL-8 expression in mice. For this, we added rhIL-8 (by intraluminal instillation) at the time of inoculation by invasive S. flexneri, or by a noninvasive control, and observed whether the addition of this chemokine altered the disease profile. We also evaluated the effect of MIP-2 and KC, two murine chemokines that are functional homologues of IL-8 and are able to promote PMN chemotaxis.

Colonic infection by invasive S. flexneri following single i.c. inoculation: graduation by Wallace and Ameho scores and histology

To evaluate the degree of tissue alteration caused by invasive Shigella, we established scores of inflammation according to the generally used criteria of Wallace and Ameho (26, 27). We present in this study the scores and histopathological features observed following 24 h of infection, and only the salient features of other time points.

Control mice infected with the noninvasive strain mxiD, at different time points after infection (i.e., 1, 2, 4, 6, and 24 h) with inocula of 109 or 1010 CFU, displayed normal macroscopic (no thickening of the colon, no apparent blood) and stereomicroscopic aspects on longitudinally opened colons (no thickening of the colonic wall, no blood, no ulceration, and no pus) similar to noninfected colon. The histological aspect was also normal with an intact epithelial lining, regular crypts of an usual size, lack of inflammatory infiltrate, absence of edema and of significant thickening of the lamina propria or muscularis, and normal and regular basal membrane (Fig. 1, A and C). Altogether, this results in low scores according to the Wallace and Ameho criteria (Table I, line 1; scores were of 1 and 2, respectively).



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  		FIGURE 1. A–D, H&E-stained sections of mouse colon, 24 h following i.c. inoculation of 109 CFU of the noninvasive S. flexneri mutant mxiD (A and C) and the invasive S. flexneri strain M90T (B and D). E–G, Immunohistochemical staining of S. flexneri with an anti-LPS specific Ab for S. flexneri, revealed by 3-amino-9-ethylcarbazol (red staining). E shows tissues infected with the noninvasive mxiD mutant, F and G show epithelium and crypts infected with the wild-type invasive strain M90T. The rectangle (F) is x2 enlarged in G.

 

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  		Table I. Evaluation of Shigella-induced inflammation on infected mouse colonsa

 
Following infection with the invasive strain M90T, at different time points of infection (1, 2, 4, 6, and 24 h), colonic sections showed occasional traces of blood following inoculation with 109 CFU. This feature appeared more frequent (~30% of the samples) following inoculation with 1010 CFU. This was confirmed by stereomicroscopic observation showing no or minimal thickening of the colon wall, hyperemia when blood traces were observed, and some scarce ulcerations of small size following inocula of 109 CFU, and more frequent following inocula of 1010 CFU; the other parameters described above being similar to the controls, as reflected by the scores of Wallace at 24 h (Table I, lines 2–4, column W; scores were of 2, 3, and 5, for 108, 109, and 1010 CFU, respectively, highlighting a dose-dependent response). Histopathological analysis showed a colonic mucosa with moderate alterations, characterized by scarce superficial and localized erosions of the epithelial lining in general associated with bacterial invasion (Fig. 1, B, D, and F, right, at 109 CFU/mouse). In a few mice, more severe alterations could be observed as soon as 3 h after infection (data not shown), with more extended epithelial erosions and even with some degree of crypt cells destruction, particularly with the highest inoculum of 1010 CFU. These erosions were associated with an inflammatory infiltrate of the lamina propria, composed only of lymphocytes and monocytes/macrophages (Fig. 1, D and F, right). Strikingly, PMN did not appear in the inflammatory infiltrate, even following 24, 48, or 72 h of infection. These observations were graded by Ameho scores 24 h after infection with the invasive strain M90T (Table I, lines 2–4, column A). Scores were of 3, 4, and 5, for 108, 109, and 1010 CFU, respectively, highlighting again a dose-dependent response, as also confirmed by cumulated scores (Wallace and Ameho, W+A), which were of 5, 7, and 10, respectively.

Labeling for Shigella-LPS and kinetics of bacterial invasion of the tissue

Labeling for Shigella-specific LPS by immunohistochemistry on tissue sections infected by the noninvasive strain mxiD failed to show bacteria in mucosal tissues, neither in the colonic epithelial lining, nor in subepithelial tissues, confirming that no significant bacterial invasion had occurred between 1 and 24 h (Fig. 1E, left). In contrast, 1 h after infection with invasive Shigella, some bacteria could already be observed in submucosal tissues, and at 90 min, a few of them were present at the bottom of crypts in the lamina propria. Following 6 h of infection, bacteria were present in large numbers in eroded areas of the epithelium lining (Fig. 1F, right) and of crypts, where infection promoted tissue alteration and destruction by plages. By contrast, following 24 h of infection, bacteria were no more observed in the epithelium, but were concentrated at the basis of crypts, in submucosal areas, as further described.

Quantification of MPO indicates no neutrophil recruitment following infection with M90T alone

The amount of MPO protein was measured by quantitative Western blot performed upon homogenized colonic tissue samples. It showed no detectable level, regardless of experimental conditions: uninfected tissues, tissues infected with the noninvasive strain mxiD, as well as tissue infected by the invasive strain M90T at all time points (Fig. 2). These results strikingly confirmed that PMN were barely recruited in the inflammatory infiltrate, even in the presence of invasive Shigella. As MPO is accurately correlated with neutrophil accumulation in tissues (5, 28), this marker was subsequently considered of primary importance to monitor PMN infiltration, and select optimal experimental conditions.



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  		FIGURE 2. Quantification of MPO by ICC (chemiluminescent detection by ECL+), in colon extracts, 24 h following i.c. delivery of either saline, or rhIL-8, or of the S. flexneri mutant mxiD, or of the invasive strain M90T, or of concomitantly inoculated rhIL-8 and Shigella (invasive or not), with or without a second injection of rhIL-8, or of rhIL-8 + Shigella. The dotted line corresponds to traces due to blood contamination in the samples. Representative experiment among three. Statistical significance, p < 0.05, n = 5.

 
The transcriptional profile of cytokines and chemokines expression in the colonic tissues suggests significant mucosal innate response to Shigella infection

Having described that the mouse colonic mucosa was permissive to a certain degree of Shigella invasion and able to elicit a mononuclear infiltrate, we established a transcriptional profile of this particular inflammatory response. We selected a battery of cytokines and chemokines genes whose profile of expression is characteristic of the inflammatory profile observed in the mouse lung infection assay (33), as well as in the rectal tissue of human experiencing shigellosis (34).

Our profile included: 1) IL-18 and IFN-{gamma}, two factors essential to protect mice against Shigella infection (35, 36, 37); 2) IL-1{beta} and TNF-{alpha}, two proinflammatory cytokines known to promote rupture of the epithelial barrier and bacterial dissemination (35, 36, 38); 3) the cytokine IL-4, reflecting the orientation of the immune innate reaction by Shigella; and 4) MIP-2 and KC, two chemokines acting as chemoattractants for PMN that are thought to compensate for the lack of IL-8 in the mouse. Evolution of the transcriptional pattern of these cytokines and chemokines was studied according to time, although only selected time points are reported in this study in Figs. 3 and 4.



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  		FIGURE 3. Time-dependent induction of cytokine and chemokine mRNAs in colon homogenates, 24 h after S. flexneri inoculation, evaluated by quantitative RT-PCR: I, IL-18; II, IFN-{gamma}; III, TNF-{alpha}; IV, IL-1{beta}; V, IL-4, after the i.c. inoculation of A: S. flexneri noninvasive mxiD, or invasive phenotype M90T, or B: of rhIL-8 alone, or of twice concomitantly inoculated rhIL-8 and Shigella. Representative experiment (n = 5) among three independent experiments. Statistical significance, p < 0.05.

 


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  		FIGURE 4. Time-dependent induction of cytokine and chemokine mRNAs in colon homogenates, after intraluminal inoculation of S. flexneri: I, KC; and II, MIP-2, evaluated by quantitative RT-PCR. A, Noninvasive S. flexneri mxiD, and invasive M90T; C, rhIL-8; or B, twice simultaneous rhIL-8 and Shigella (invasive or not). Representative experiment (n = 5) among three independent experiments. Statistical significance, p < 0.05.

 
IL-18 transcripts were constitutively expressed at high level in the colonic tissue of noninfected samples or of tissues infected with the noninvasive strain mxiD. Upon infection with the invasive strain M90T, IL-18 transcription increased at 1 h, then started to decrease, with significant reduction at 4 h, and almost extinction at 24 h (Fig. 3IA). A similar pattern of transcriptional down-regulation was observed, although less marked, with IFN-{gamma} (Fig. 3IIA).

In contrast, transcripts for TNF-{alpha} and IL-1{beta} were not expressed by colonic tissues, either not infected, or infected by the noninvasive mutant mxiD. Following infection with the invasive strain M90T, transcripts could already be detected at 1 h and continued to increase at 2 h (TNF-{alpha}, Fig. 3IIIA; IL-1{beta}, Fig. 3IVA). IL-1{beta} transcripts then subsequently decreased, whereas TNF-{alpha} transcripts kept increasing until 24 h. IL-4 transcripts were strongly induced at 6–24 h after infection with the invasive strain M90T (Fig. 3VA), compared with the noninvasive strain mxiD. Transcripts for MIP-2 and KC were not detected in noninfected tissues or tissues infected by the noninvasive strain mxiD. In contrast, MIP-2 and KC transcripts started to increase 1 h following infection with the invasive strain M90T and kept increasing to peak at 24 h (Fig. 4, IA and IIA, respectively).

Strategy retained to study the effects of rhIL-8 added to Shigella

To study whether the concomitant administration of IL-8 and Shigella was able to induce a profile of infection mimicking the disease observed in humans, rhIL-8 was delivered intraluminally on the epithelial surface of the colon. In one group of mice, rhIL-8 and the Shigella inoculum were instilled concomitantly (invasive strain or noninvasive control). In other groups of mice, a second administration was performed 18 h following the first administration, either of rhIL-8 alone, or of rhIL-8 and Shigella. Colons were collected following the sacrifice of animals at 24 h. MPO was retained as a first criterion for quantifying neutrophil recruitment and deciding upon the best protocol of instillation of bacteria and rhIL-8.

Optimization of the protocol of administration of Shigella and rhIL-8

The amount of MPO expressed in infected tissues was selected as a read out to evaluate the number of PMN in the inflammatory infiltrate. Fig. 2 (column 5) shows that the amount of MPO was not significantly increased in colon extracts at 24 h following rhIL-8 plus M90T instillation, compared with M90T alone (column 4), and to the control associating mxiD and rhIL-8 (columns 1–3). However, when the first i.c. administration of M90T plus IL-8 was followed, 18 h later, by a second instillation of rhIL-8 alone, the amount of MPO measured appeared significantly increased (Fig. 2, column 7), and increased even more when both M90T and rhIL-8 were readministered (Fig. 2, column 9), compared with the amount of MPO in mice receiving the corresponding control mxiD plus rhIL-8 (columns 6 and 8, respectively). Thus, the latter condition encompassing two administrations of M90T plus rhIL-8, 18 h apart, appeared optimal and was selected for additional experiments.

Combination of invasive Shigella and rhIL-8 cause neutrophilic inflammation in infected colons.

Control mice instilled with rhIL-8 (2 µg) alone, once or twice, did not show any significant histological alterations (Fig. 5A), and rhIL-8 instilled at doses up to 1.5–2 µg, together with the noninvasive strain mxiD did not induce significant alteration either, even when the dose was repeated 18 h later (Fig. 5B). This led to low scores of Wallace and Ameho (Table I, lines 6 and 7; scores were of 3 and 4, and 4 and 4, respectively). In contrast, when coadministration of invasive strain M90T and rhIL-8 was performed twice, at 18-h interval, according to the protocol selected, we observed a dramatic increase in colon wall thickening (doubled, or x4), in extent of thickening (up to 20 mm length, exceptionally more, reflecting pancolitis), edema, and blood associated with extensive ulcerations. This translated in increased Wallace scores (Table I, W, lines 8–10: scores were of 7.5, 10, and 12 for 108, 109, and 1010 CFU, respectively), indicating a marked dose-dependent response. Histopathological observation confirmed a dramatic thickening of the colon wall, the presence of erosions (Fig. 5, C and E, compared with Fig. 5B), destruction of the epithelial lining and of the basal membrane, and deep alteration and destruction of the structure of crypts, such as shortening, rounding (Fig. 5C, middle), and some tissue necrosis. Edema was observed, associated with a marked infiltration by numerous neutrophils in the lamina propria, and at the basis of crypts, in addition to other inflammatory cells such as lymphocytes, monocytes/macrophages (Fig. 5, C and D), and inflammation-associated lymphoid (immune cell) aggregate (Fig. 5C, high part). These massive alterations, now characterized by an inflammatory infiltrate dominated by PMN, translated into much higher Ameho scores (Table I, A, lines 8–10). For 108, 109, and 1010 CFU, cumulated scores (W+A) were of 15.5, 20, and 23, respectively.



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  		FIGURE 5. H&E-stained sections of mouse colon, 24 h following i.c. inoculation with rhIL-8 alone (A), 109 CFU of the noninvasive S. flexneri mutant mxiD + rhIL-8 (B), 109 CFU of the invasive S. flexneri strain M90T + rhIL-8 (C and D at higher magnification) inoculated i.c. at to and at t0 + 18 h, and collected at t0 + 24 h. E, Right part of C, at higher magnification.

 
As expected, based on MPO expression data, the protocol encompassing a single concomitant instillation of M90T and rhIL-8 showed only rare PMN in the inflammatory infiltrate (Fig. 2).

ICC shows a colocalization of invasive Shigella with PMN in samples treated twice with rhIL-8 and M90T

At 6 h following noninvasive Shigella infection, the control 2 x (mxiD + IL-8) showed no labeling (Fig. 6A). In contrast, mice infected with invasive M90T showed a marked LPS labeling at the site where the lining epithelium was completely eroded, and abscesses were observed (Fig. 6B), which promote necrosis, as observed at the basis of crypts (Fig. 6B, bottom).



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  		FIGURE 6. ICC for LPS of S. flexneri in colon sections (A and B) at 6 h following intraluminal inoculation of noninvasive strain mxiD (A), or invasive M90T at 109 CFU/mouse concomitant with rhIL-8 (2 µg) (B); or (C–F) at 24 h following the intraluminal instillation of noninvasive strain mxiD (C); invasive M90T at 109 CFU/mouse concomitant with rhIL-8 (2 µg) (D and E); M90T at 1010 CFU/mouse + IL-8 (2 µg) (F). In E and F (left side), arrows indicate PMN. In F (right), the arrow indicates an infected PMN (labeled in red with Shigella-LPS).

 
At 24 h following Shigella infection, colonic sections from animals infected with control strain mxiD (2 x inoculated), or mxiD +rhIL-8 (2 x) showed no labeling for Shigella LPS (Fig. 6C), whereas with invasive Shigella (108 to 1010 CFU, 2 x M90T + rhIL-8), a marked dose-dependent labeling with specific LPS was observed at the base of crypts, (Fig. 6, at 109 CFU, D, E, and at 1010 CFU, F), which colocalized with PMN, observed by hematoxylin counterstain (Fig. 6F, basis of crypts, right arrow).

Cytokine and chemokine transcriptional profiles after infection with twice (rhIL-8 plus M90T)

Intraluminal rhIL-8 instillation, induced dose-dependent transcription of some cytokines, such as IL-18, TNF–{alpha} (Fig. 3B, for both cytokines), and KC and MIP-2 mRNAs (Fig. 4, IC and IIC). Consequently, the synthesis of TNF-{alpha} following 2 x (rhL8 + M90T), was markedly increased (at 24 h, Fig. 3IIIB), as well as KC and MIP-2 synthesis (Fig. 4, IB and IIB).

rmKC or rmMIP-2 used in the same conditions as IL-8 failed to induce neutrophil recruitment at the site of infection

The amount of MPO protein measured by quantitative Western blot performed upon whole colon homogenates from mice treated by rmKC or rmMIP-2 (2 µg) and invasive S. flexneri, in the same conditions as these set for rhIL-8 (2 x 2 µg of the chemokine), failed to increase (Fig. 7). Higher doses of the chemokines were not suitable because they induced alterations in the control, and 1 x 4 µg did not induce significant PMN recruitment. Histopathological observation confirmed the lack of neutrophils in these experimental conditions (data not shown).



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  		FIGURE 7. Quantification of MPO protein by Western blot in colon extracts (as in Fig. 2), 24 h following i.c. delivery of the recombinant murine chemokines KC or MIP-2 concomitantly with Shigella (109 CFU/mouse). Control mxiD + rmKC, or invasive strain M90T + rmKC, were instilled once, or twice, as well as using rmMIP-2 (n = 5). The dotted line represents blood traces, i.e., the basal level. Note that no condition promoted MPO expression as observed following 2 x (rhIL-8 +M90T) (Fig. 4). Similar results were obtained using 1010 CFU/mouse (data not shown).

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
Even though the refractory state of the mouse bowel to develop an acute destructive rectocolitis in the presence of invasive Shigella is a major handicap to pathogenesis and vaccine studies, it is also a challenging enigma, the resolution of which may bring about crucial information upon the pathogenic mechanisms of shigellosis. This refractory state is in contrast with the massive inflammation elicited by Shigella when inoculated into the respiratory tract (2, 33) (i.e., acute bronchoalveolitis), thereby providing an open system in which bacteria can establish straight contact with cells of the innate immune system, such as alveolar macrophages. It also contrasts with the susceptibility of newborn mice during a short time window of 4 days, during which pups develop acute proximal enteritis following their intragastric inoculation, probably due to immaturity of their epithelial barrier (3). Conversely, in a system characterized by a tight barrier such as the colonic epithelium of adult mice, the lack of reactivity to Shigella is likely to be linked to the inability of this pathogen to unlock this barrier, and to reverse the state of bowel tolerance to the intestinal flora that is particularly dense in the colon.

To bypass the transit of bacteria through the whole gastrointestinal tract, we have chosen the i.c. route to deliver Shigella directly in contact with the colonic epithelium, thus avoiding unwanted interferences that may severely reduce the bacterial inoculum (6, 21). This model allowed us to demonstrate that in BALB/cJ mice, the colonic mucosa can be invaded by virulent Shigella, indicating that the refractory state does not reside in an inability of bacteria to penetrate epithelial cells. Furthermore, invading bacteria elicit a significant degree of cytotoxicity to the epithelium and recruit an inflammatory infiltrate that, however, remains modest and exclusively composed of mononuclear cells. The striking feature of this infectious pattern is the lack of a PMN infiltrate, thus the lack of massive rupture and subsequent inflammatory destruction of the intestinal epithelium that is characteristic of the experimental lesions observed in the rabbit ileal loops (7) and the clinical lesions observed in humans (39). The inability of the mouse to promote marked PMN inflammation in response to Shigella invasion suggested that the murine defect resided more in the sensory and effector program of the epithelium. Preliminary experiments in which we tried to prime the colonic epithelium with a chemical agent, such as ethanol, were unsuccessful. Thus, we decided to investigate the hypothesis that the lack of IL-8 in mice may largely account for the absence of further development of the infectious process (7, 14).

IL-8 in humans is produced by numerous cell types, particularly monocytes/macrophages, epithelial cells, neutrophils, fibroblasts, and endothelial cells upon infection by bacteria or stimulation by bacterial products (i.e., pathogen-associated molecular patterns), or stimulated by cytokines such as IL-1{beta} and TNF-{alpha} (40, 41). IL-8 is mainly active on neutrophils, promoting their recruitment and also their strong activation characterized by activation of the leukotriene pathway (42), by the release of their granular content (i.e., elastase (43), lactoferrin (44), and MPO), and their increased adherence to endothelial cells, as well as NO activation (19, 40, 41, 42, 45). IL-8 is also a chemoattractant for other cell types such as basophils, T lymphocytes, and NK cells, and also enhances permeability of endothelial cells (19, 40, 45). In ulcerative colitis whose histopathological lesions strongly resemble those observed in shigellosis, the level of IL-8 expression correlates with the severity of the disease (46, 47), particularly with neutrophilic inflammation and mucosal destruction.

Interestingly, when rhIL-8 was administered to the mouse, together with invasive Shigella, the disease gained a profile similar to human shigellosis and to the disease observed in animal models such as the rabbit ligated ileal loop model (7). The whole histopathological pattern became characteristic of bacillary dysentery, with a marked rupture of the epithelial barrier, massive bacterial invasion of the epithelium and subjacent tissues, and a marked inflammation again characterized by a dominant PMN infiltrate, but also associated to mononuclear cells and to dramatic tissue injury (deep erosion, crypt alterations, necrosis, edema).

Indeed, bacteria appeared massively present in areas of epithelial destruction in the immediately subjacent lamina propria, but also at the basis of the crypts, 24 h following inoculation. The reason for this localization is unclear, although it has already been observed in guinea pigs (8). One possibility is that bacteria may have penetrated through the crypt epithelium or via M cells (48). Alternatively, Shigella may have been transported by migrating cells from the colonic lumen to the lamina propria, such as CD18-expressing phagocytes (49), or by dendritic cells recruited at the site of epithelial colonization, that may directly perform uptake of bacteria across the epithelial lining. Indeed, these latter cells may open the tight junctions between epithelial cells, send dendrites outside the epithelium to sample bacteria from the lumen, and subsequently carry them as they migrate toward the basal lamina propria on their way to lymphoid organs (50).

In any event, Shigella were also associated in these areas with a PMN infiltrate, which may be due to a chemotactic gradient formed through the crypt epithelium by intraluminal IL-8, or caused by the bacteria themselves, following local activation of MIP-2 or KC production and/or production of microbial chemoattractants for PMN, such as fMLP, formylnorleucyl-leucyl-phenylalanine, or bacterial peptides (51, 52, 53, 54).

This situation is characteristic of a system in which bacteria are likely to make use of PMN to disrupt the epithelial lining and invade the epithelium and subjacent tissues. It agrees with a model of loop amplification (i.e., vicious circle) previously described, according to which bacterial invasion, following strong signaling for IL-8, promotes recruitment of a PMN infiltrate that leads to the rupture of the epithelium, its destruction promoting further Shigella invasion (55). In this model, the PMN infiltrate eventually leads to bacterial elimination through efficient generic antibacterial mechanisms, including production of lactoferrin (44), and more specific mechanisms, such as the degradation of Shigella virulence effectors by released elastase that disarms the pathogen (43). Thus, in the IL-8-mediated loop, massive tissue destruction appears to be the cost of final bacterial eradication, thereby dictating the pathologic profile of shigellosis.

In addition to mimicking the histopathological profile of shigellosis, addition of IL-8 also generated a relevant pattern of cytokine and chemokine transcription that agrees with the known mechanisms of innate response against Shigella. Interestingly, Shigella appeared to quickly down-regulate the expression of IL-18 and IFN-{gamma}, which are critical in mediating the protection of mice and the growth of Shigella in tissues at the initial stage of the innate immune response (35, 36, 37, 56). Absence of IL-18 has been shown to severely impair the capacity of infected mice to eradicate a Shigella or an Escherichia coli inoculum (38, 57), and mice devoid of the IFN-{gamma} receptor appear highly susceptible to Shigella infection (37). Therefore, it would make sense that Shigella, quickly following epithelial invasion, turn down IL-18 expression, thereby down-regulating expression of IFN-{gamma}, particularly by NK cells. This is consistent with the observation that, in the rectal mucosa of Shigella-infected patients, IFN-{gamma}-producing cells appear dramatically reduced in number (34), thus adding relevance to the current mouse model. Conversely, Shigella invasion in mice led to the development of a clear proinflammatory profile characterized by the induction of genes coding for IL-1{beta} and TNF-{alpha}. Both cytokines participate in the previously described amplification loop through their ability to induce disruption of the epithelial barrier (35, 36, 38, 51, 58, 59). In particular, these cytokines promote inflammation via the expression of adhesion molecules and chemokines favoring recruitment of cells, such as PMN and NK cells (60), thereby enhancing tissue injury. Finally, the induction of IL-4 transcription may reflect a quickly established tip in the Th1/Th2 balance of the mucosal response, in agreement with the dominance of the humoral adaptative protection against Shigella infection (61). MIP-2 and KC, the IL-8 homologues in the mouse, were also induced following Shigella infection, but were still unable to promote significant PMN recruitment to a point allowing mice to develop colitis. The reasons for this are unclear. Perhaps the invasive process by Shigella is not sufficient in the mouse epithelium to elicit expression of MIP-2 and KC at the critical level required for activation of CXCR2 and signal transduction, allowing to recruit a sufficient PMN infiltrate. Alternatively, MIP-2 and KC may be not as potent chemoattractants as IL-8 in mouse (62, 63). Finally, expression of the three chemokines in conjunction might be required for CXCR2 activation then effective PMN recruitment, as suggested using transgenic IL-8 mice and anti-MIP-2 or anti-KC Abs (64). Thus, IL-8 is likely to remain the predominant effector, because ectopic addition of either rmMIP-2 or rmKC, both chemotactic for PMN in vitro and in vivo (65), but with various efficiencies (65, 66), failed to promote PMN recruitment in the same conditions of those defined for rhIL-8.

In conclusion, the model seems to adequately reflect the human disease, with some limitations, among which the necessity to administer two doses of a high bacterial inoculum (i.e., 109 CFU compared with the 102-103 CFU required to cause disease in humans), in addition to IL-8. This possibly reflects the short half-life of the rhIL-8 signal in the colonic lumen, providing a very short-lived luminal-mucosal gradient, insufficient to promote PMN recruitment. This might also reflect weak efficiency of IL-8, possibly due to a lower affinity of the ligand IL-8-CXCR2 receptor system from nonhomologous species (human-mouse), or to the lack of CXCR1-receptor. It is likely that more sustained expression of IL-8 in the presence of Shigella invasion of the colonic epithelium would achieve a more prolonged gradient, allowing use of a lower Shigella inoculum.

In addition, the experimental procedure might also shortcut a crucial signaling function of the epithelial barrier. For instance, in the case of Salmonella infection, it has been shown that a chemoattractant gradient (i.e., pathogen-elicited epithelial chemoattractant activity) was created apico basally following apical interaction of the invasive microorganism with intestinal epithelial cells (67). This gradient has been shown to account for the basoapical transmigration of the PMN, themselves recruited to the basal side of the epithelium by the basomucosal gradient formed by IL-8 produced by epithelial cells (67, 68). If Shigella were unable to cause production of an equivalent pathogen-elicited epithelial chemoattractant activity gradient in mice, compared with humans, it may explain the partial effect observed in mice by adding IL-8 apically. A more sustained level of IL-8 expression with the generation of a gradient allowing PMN recruitment at the site of infection is needed, suggesting use of transgenic mice whose IL-8 expression would be induced following Shigella infection, in the colonic epithelium. Despite these limitations, the current model constitutes a major step forward in the development of a murine model of bacillary dysentery.


   Acknowledgments
 
We thank John Rohde for the critical reading of the manuscript.


   Footnotes
 
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

1 Address correspondence and reprint requests to Dr. Monique Singer, Unité de Pathogénie Microbienne Moléculaire, Institut National de la Santé et de la Recherche Médicale U389, Institut Pasteur, 25 rue du Dr Roux, 75015, Paris, France. E-mail address: msinger{at}pasteur.fr Back

2 P.J.S. is a Howard Hughes Medical Institute Scholar. Back

3 Abbreviations used in this paper: PMN, polymorphonuclear leukocytes; MPO, myeloperoxidase; ICC, immunocytochemistry; i.c., intracolonic; rh, recombinant human; KC, keratinocyte-derived cytokine; rm, recombinant murine. Back

Received for publication March 31, 2004. Accepted for publication July 2, 2004.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

  1. Hale, T. L.. 1988. Bacilliary dysentery. W.J. Hansler, and M. Shuman, eds. In Topley and Wilson’s Microbiology and Microbial Infections Vol. 3:479. Arnold, London.
  2. Voino-Yasenetsky, M. V., M. K. Voino-Yasenetskaya. 1962. Experimental pneumonia caused by bacteria of the Shigella group. Acta Morphol. Acad. Sci. Hung. 11:439.[Medline]
  3. Fernandez, M. I., A. Thuizat, T. Pedron, M. Neutra, A. Phalipon, P. J. Sansonetti. 2003. A newborn mouse model for the study of intestinal pathogenesis of shigellosis. Cell. Microbiol. 5:481.[Medline]
  4. Bhan, A. K., E. Mizoguchi, R. N. Smith, A. Mizoguchi. 1999. Colitis in transgenic and knockout animals as models of human inflammatory bowel disease. Immunol. Rev. 169:195. Review. [Medline]
  5. Desrumeaux, P., L. Dubuquoy, S. Nutten, M. Peuchmaur, W. Englaro, K. Schoonjans, B. Derijard, B. Desvergne, W. Wahli, P. Chambon, et al 2001. Attenuation of colon inflammation through activators of the retinoid X receptor (RXR)/peroxisome proliferator-activated receptor {gamma} (PPAR{gamma}) heterodimer: a basis for new therapeutic strategies. J. Exp. Med. 193:827.[Abstract/Free Full Text]
  6. Boirivant, M., I. J. Fuss, A. Chu, W. Strober. 1998. Oxazolone colitis: a murine model of T helper cell type 2 colitis treatable with antibodies to interleukin 4. J. Exp. Med. 188:1929.[Abstract/Free Full Text]
  7. Sansonetti, P. J., J. Arondel, M. Huerre, A. Harada, K. Matsushima. 1999. Interleukin-8 controls bacterial transepithelial translocation at the cost of epithelial destruction in experimental shigellosis. Infect. Immun. 67:1471.[Abstract/Free Full Text]
  8. Bernardini, M. L., J. Arondel, I. Martini, A. Aidara, P. J. Sansonetti. 2001. Parameters underlying successful protection with live attenuated mutants in experimental shigellosis. Infect. Immun. 69:1072.[Abstract/Free Full Text]
  9. Girardin, S. E., D. J. Philpott, B. Lemaitre. 2003. Sensing microbes by diverse hosts: workshop on pattern recognition proteins and receptors. EMBO Rep. 4:932.[Medline]
  10. Girardin, S. E., I. G. Boneca, L. A. Carneiro, A. Antignac, M. Jehanno, J. Viala, K. Tedin, M. K. Taha, A. Labigne, U. Zahringer, et al 2003. Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science 300:1584.[Abstract/Free Full Text]
  11. Chamaillard, M., M. Hashimoto, Y. Horie, J. Masumoto, S. Qiu, L. Saab, Y. Ogura, A. Kawasaki, K. Fukase, S. Kusumoto, et al 2003. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol. 4:702.[Medline]
  12. Philpott, D. J., S. Yamaoka, A. Israel, P. J. Sansonetti. 2000. Invasive Shigella flexneri activates NF-{kappa}B through a lipopolysaccharide-dependent innate intracellular response and leads to IL-8 expression in epithelial cells. J. Immunol. 165:903.[Abstract/Free Full Text]
  13. Pédron, T., C. Thibault, P. J. Sansonetti. 2003. The invasive phenotype of Shigella flexneri directs a distinct pattern in the human intestinal epithelial cell line Caco-2. J. Biol. Chem. 278:33878.[Abstract/Free Full Text]
  14. Modi, W. S., T. Yoshimura. 1999. Isolation of novel GRO genes and phylogenetic analysis of the CXC chemokine subfamily in mammals. Mol. Biol. Evol. 16:180.[Abstract]
  15. Watanabe, K. M., K. Lida, K. Takaishi, T. Suzuki, Y. Hamada, Y. Lizuka, S. Tsurufuji. 1993. Chemoattractants for neutrophils in lipopolysaccharide-induced inflammatory exudate from rats are not interleukin-8 counterparts but GRO-gene product/melanoma-growth-stimulating-activity-related factors. Eur. J. Biochem. 214:267.[Medline]
  16. Rovai, L. E., H. R. Herschman, J. B. Smith. 1998. The murine neutrophil-chemoattractant chemokines LIX, KC, and MIP-2 have distinct induction kinetics, tissue distributions, and tissue-specific sensitivities to glucocorticoid regulation in endotoxemia. J. Leukocyte Biol. 64:494.[Abstract]
  17. Bozic, C. R., N. P. Gerard, C. von Uexkull-Guldenband, L. F. Kolakowski, Jr, M. J. Conklyn, R. Breslow, H. J. Showell, C. Gerard. 1994. The murine interleukin 8 type B receptor homologue and its ligands: expression and biological characterization. J. Biol. Chem. 269:29355.[Abstract/Free Full Text]
  18. Bozic, C. R., L. F. Kolakowski, Jr, N. P. Gerard, C. Garcia-Rodriguez, C. von Uexkull-Guldenband, M. J. Conklyn, R. Breslow, H. J. Showell, C. Gerard. 1995. Expression and biologic characterization of the murine chemokine KC. J. Immunol. 154:6048.[Abstract]
  19. Nourshargh, S., J. A. Perkins, H. J. Showell, K. Matsushima, T. J. Williams, P. D. Collins. 1992. A comparative study of the neutrophil stimulatory activity in vitro and pro-inflammatory properties in vivo of 72 amino acid and 77 amino acid IL-8. J. Immunol. 14:106.
  20. Rennen, H. J., O. C. Boerman, W. J. Oyen, F. H. Corstens. 2003. Kinetics of 99mTc-labeled interleukin-8 in experimental inflammation and infection. J. Nucl. Med. 44:1502.[Abstract/Free Full Text]
  21. Neurath, M. F., I. Fuss, B. L. Kelsall, D. Presky, H. Waegell. 1996. Experimental granulomatous colitis in mice is abrogated by induction of TGF-{beta}-mediated oral tolerance. J. Exp. Med. 183:2605.[Abstract/Free Full Text]
  22. Allaoui, A., P. J. Sansonetti, C. Parsot. 1993. MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri Ipa invasins. Mol. Microbiol. 7:59.[Medline]
  23. Sansonetti, P. J., H. d’Hauteville, S. B. Formal, M. Toucas. 1982. Plasmid-mediated invasiveness of "Shigella-like". Escherichia coli. Ann. Microbiol. (Paris) 133:351.
  24. Maurelli, A. T., P. J. Sansonetti. 1988. Identification of a chromosomal gene controlling temperature-regulated expression of Shigella virulence. Proc. Natl. Acad. Sci. USA 85:2820.[Abstract/Free Full Text]
  25. Nozawa, Y., K. Nishihara, R. M. Peek, M. Nakano, T. Uji, H. Ajioka, N. Matsuura, H. Miyake. 2002. Identification of a signaling cascade for interleukin-8 production by Helicobacter pylori in human gastric epithelial cells. Biochem. Pharmacol. 64:21.[Medline]
  26. Wallace, J. L., W. K. MacNaughton, G. P. Morris, P. L. Beck. 1989. Inhibition of leukotriene synthesis markedly accelerates healing in a rat model of inflammatory Bowel disease. Gastroenterology 96:29.[Medline]
  27. Ameho, C. K., A. A. Adjei, K. Harrison, K. Takeyeshita, T. Morioka, Y. Arakaki, E. Ito, I. Suzuki, A. D. Kulkarni, S. Kawajiri, S. Yamamoto. 1997. Prophylactic effects of dietary glutamine supplementation on interleukin-8 and tumour necrosis factor a production in trinitrobenzene sulphonic acid induced colitis. Gut 41:487.[Abstract/Free Full Text]
  28. Singer, M., J. Lefort, B. B. Vargaftig. 2002. Granulocyte depletion and dexamethasone differentially modulate airways hyperreactivity, inflammation mucus accumulation and secretion induced by rmIL-13 or antigen. Am. J. Respir. Cell Mol. Biol. 26:74.[Abstract/Free Full Text]
  29. Vargaftig, B. B., M. Singer. 2003. Leukotrienes mediate murine bronchopulmonary hyperreactivity, inflammation, and part of mucosal metaplasia and tissue injury induced by recombinant murine interleukin-13. Am. J. Respir. Cell Mol. Biol. 28:410.[Abstract/Free Full Text]
  30. Colle, J. H., P. Falanga, M. Singer, B. Hevin, G. Milon. 1999. Quantification of messenger RNA by competitive RT-PCR: a simplified read out assay. J. Immunol. Methods 210:175.
  31. Vargaftig, B. B., M. Singer. 2003. Leukotrienes mediate part of Ova-induced lung effects in mice via EGFR. Am. J. Physiol. 285:L808.
  32. Vargaftig, B. B., M. Singer. 2003. Leukotrienes, IL-13 and chemokines cooperate for BHR and mucipare metaplasia in allergic mouse lungs. Am. J. Physiol. 284:L260.
  33. Cersini, A., M. C. Martino, I. Martini, G. Rossi, M. L. Bernardini. 2003. Analysis of virulence and inflammatory potential of Shigella flexneri purine biosynthesis mutants. Infect. Immun. 71:7002.[Abstract/Free Full Text]
  34. Raqib, R., A. Gustafsson, J. Andersson, M. Bakhiet. 1997. A systemic downregulation of {gamma} interferon production is associated with acute shigellosis. Infect. Immun. 65:5338.[Abstract]
  35. Sansonetti, P. J., J. Arondel, J. M. Cavaillon, M. Huerre. 1995. Role of interleukin-1 in the pathogenesis of experimental shigellosis. J. Clin. Invest. 96:884.
  36. Sansonetti, P. J., A. Phalipon, J. Arondel, K. Thirumalai, S. Banerjee, S. Akira, K. Takeda, A. Zychlinsky. 2000. Caspase-1 activation of IL-1{beta} and IL-18 are essential for Shigella flexneri-induced inflammation. Immunity 12:581.[Medline]
  37. Way, S. S., M. B. Goldberg. 1998. Clearance of Shigella flexneri infection occurs through a nitric oxide-independent mechanism. Infect. Immun. 66:3012.[Abstract/Free Full Text]
  38. Guy-Grand, D., J. P. DiSanto, P. Henchoz, M. Malassis-Seris, P. Vassalli. 1998. Small bowel enteropathy: role of intraepithelial lymphocytes and of cytokines (IL-12, IFN-{gamma}, TNF) in the induction of epithelial cell death and renewal. Eur. J. Immunol. 28:730.[Medline]
  39. Mathan, V. I., M. M. I. Mathan. 1991. Intestinal manifestations of invasive diarrheas and their diagnosis. Rev. Infect. Dis. 13:S311.
  40. Baggiolini, M., B. Dewald, B. Moser. 1994. Interleukin-8 and related chemotactic cytokines: CXC and CC chemokines. Adv. Immunol. 55:197.
  41. Mukaida, N., A. Hishinuma, C. O. Zachariae, J. J. Oppenheim, K. Matsushima. 1991. Regulation of human interleukin 8 gene expression and binding of several other members of the intercrine family to receptors for interleukin-8. Adv. Exp. Med. Biol. 305:31.[Medline]
  42. Fogh, K., C. G. Larsen, L. Iversen, K. Kragballe. 1992. Interleukin-8 stimulates the formation of 15-hydroxy-eicosatetraenoic acid by human neutrophils in vitro. Agents Actions. 35:227.[Medline]
  43. Weinrauch, Y., D. Drujan, S. D. Shapiro, J. Weiss, A. Zychlinsky. 2002. Neutrophil elastase targets virulence factors of enterobacteria. Nature 417:91.[Medline]
  44. Gomez, H. F., T. J. Ochoa, L. G. Carlin, T. G. Cleary. 2003. Human lactoferrin impairs virulence of Shigella flexneri. J. Infect. Dis. 187:87.[Medline]
  45. Mukaida, N., A. Harada, K. Matsushima. 1998. Interleukin-8 (IL-8) and monocyte chemotactic and activating factor (MCAF/MCP-1), chemokines essentially involved in inflammatory and immune reactions. Cytokine Growth Factor Rev. 9:9.[Medline]
  46. Izzo, R. S., K. Witkon, A. I. Chen, C. Hadjiyane, M. I. Weinstein, C. Pellecchia. 1992. Interleukin-8 and neutrophil markers in colonic mucosa from patients with ulcerative colitis. Am. J. Gastroenterol. 87:1447.[Medline]
  47. Mazzucchelli, L., C. Hauser, K. Zgraggen, H. Wagner, M. Hess, J. A. Laissue, C. Mueller. 1994. Expression of interleukin-8 gene in inflammatory bowel disease is related to the histological grade of active inflammation. Am. J. Pathol. 144:997.[Abstract]
  48. Sansonetti, P. J., A. Phalipon. 1999. M cells as ports of entry for enteroinvasive pathogens: mechanisms of interaction, consequences for the disease process. Semin. Immunol. 11:193.[Medline]
  49. Vazquez-Torres, A., J. Jones-Carson, A. J. Baumler, S. Falkow, S. Valdivia, W. Brown, R. Le, M. Berggren, W. T. Parks, F. C. Fang. 1999. Extraintestinal dissemination of Salmonella by CD18-expressing phagocytes. Nature 401:804.[Medline]
  50. Rescigno, M., G. Rotta, B. Valzasina, P. Ricciardi-Castagnoli. 2001. Dendritic cells shuttle microbes across gut epithelial monolayers. Immunobiology 204:572.[Medline]
  51. D’Hauteville, H., S. Khan, D. J. Maskell, A. Kussak, A. Weintraub, J. Mathison, R. J. Ulevitch, N. Wuscher, C. Parsot, P. J. Sansonetti. 2002. Two msbB genes encoding maximal acylation of lipid A are required for invasive Shigella flexneri to mediate inflammatory rupture and destruction of the intestinal epithelium. J. Immunol. 168:5240.[Abstract/Free Full Text]
  52. Henderson, B., S. Poole, M. Wilson. 1996. Bacterial modulins: a novel class of virulence factors which cause host tissue pathology by inducing cytokine synthesis. Microbiol. Rev. 60:316.[Abstract/Free Full Text]
  53. Wilson, M., R. Seymour, B. Henderson. 1998. Bacterial perturbation of cytokine networks. Infect. Immun. 66:2401.[Free Full Text]
  54. Russo, T. A., B. A. Davidson, D. M. Topolnycky, R. Olson, S. A. Morrill, P. R. Knight, P. M. Murphy. 2003. Human neutrophil chemotaxis is modulated by capsule and O-antigen from an extraintestinal pathogenic Escherichia coli strain. Infect. Immun. 71:6435.[Abstract/Free Full Text]
  55. Perdomo, O. J., J. M. Cavaillon, M. Huerre, H. Ohayon, P. Gounon, P. J. Sansonetti. 1994. Acute inflammation causes epithelial invasion and mucosal destruction in experimental shigellosis. J. Exp. Med. 180:1307.[Abstract/Free Full Text]
  56. Hilbi, H., J. E. Moss, D. Hersh, Y. Chen, J. Arondel, S. Banerjee, R. A. Flavell, J. Yuan, P. J. Sansonetti, A. Zychlinsky. 1998. Shigella-induced apoptosis is dependent on caspase-1 which binds to IpaB. J. Biol. Chem. 273:32895.[Abstract/Free Full Text]
  57. Weijer, S., M. E. Sewnath, A. F. Vos, S. Florquin, K. van der Sluis, D. J. Gouma, K. Takeda, S. Akira, T. van der Poll. 2003. Interleukin-18 facilitates the early antimicrobial host response to Escherichia coli peritonitis. Infect. Immun. 71:5488.[Abstract/Free Full Text]
  58. Perdomo, J. J., P. Gounon, P. J. Sansonetti. 1994. Polymorphonuclear leukocyte transmigration promotes invasion of colonic epithelial monolayer by Shigella flexneri. J. Clin. Invest. 93:633.
  59. Madara, J. L., A. M. Hopkins, A. Nusrat. 2003. Proinflammatory cytokines disrupt epithelial barrier function by apoptosis-independent mechanisms. J. Immunol. 171:6164.[Abstract/Free Full Text]
  60. Netea, M. G., B. J. Kullberg, I. Verschueren, J. W. Van Der Meer. 2000. Interleukin-18 induces production of proinflammatory cytokines in mice: no intermediate role for the cytokines of the tumor necrosis factor family and interleukin-1{beta}. Eur. J. Immunol. 30:3057.[Medline]
  61. Phalipon, A., M. Kaufmann, P. Michetti, J. M. Cavaillon, M. Huerre, P. J. Sansonetti, J. P. Kraehenbuhl. 1995. Monoclonal immunoglobulin A antibody directed against serotype-specific epitope of Shigella flexneri lipopolysaccharide protects against murine experimental shigellosis. J. Exp. Med. 182:769.[Abstract/Free Full Text]
  62. Rot, A.. 1991. Chemotactic potency of recombinant human neutrophil attractant/activation protein-1 (interleukin-8) for polymorphonuclear leukocytes of different species. Cytokine 3:21.[Medline]
  63. Lee, J., G. Cacalano, T. Camerato, K. Toy, M. W. Moore, W. I. Wood. 1995. Chemokine binding and activities mediated by the mouse IL-8 receptor. J. Immunol. 155:2158.[Abstract]
  64. Remick, D. J., L. B. Green, D. E. Newcomb, S. J. Garg, G. L. Bolgos, D. Ruben Call. 2001. CXC Chemokine redundancy ensures local neutrophil recruitment during acute inflammation. Am. J. Pathol. 159:1149.[Abstract/Free Full Text]
  65. Gupta, S., L. Feng, T. Yoshimura, J. Redick, S. M. M. Fu, C. E. Rose, Jr. 1996. Intra-alveolar macrophage-inflammatory peptide 2 induces rapid neutrophil localization in the lung. Am. J. Respir. Cell Mol. Biol. 15:656.[Abstract]
  66. Tessier, P. A., P. H. Naccache, K. R. Diener, R. P. Gladue, K. S. Neote, I. Clark-Lewis, S. R. McColl. 1998. Induction of acute inflammation in vivo by staphylococcal superantigens. II. Critical role for chemokines, ICAM-1 and TNF-{alpha}. J. Immunol. 161:1204.[Abstract/Free Full Text]
  67. McCormick, B. A., C. A. Parkos, S. P. Colgan, D. K. Carnes, J. L. Madara. 1998. Apical secretion of a pathogen-elicited epithelial chemoattractant activity in response to surface colonization of intestinal epithelia by Salmonella typhimurium. J. Immunol. 160:455.[Abstract/Free Full Text]
  68. Kucharzik, T., I. R. Williams. 2002. Neutrophil migration across the intestinal epithelial barrier-summary of in vitro data and description of a new transgenic mouse model with doxycycline-inducible interleukin-8 expression in intestinal epithelial cells. Pathobiology 70:143.[Medline]



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