Strong and Durable TCR Clustering at the T/Dendritic Cell Immune Synapse Is Not Required for NFAT Activation and IFN- Production in Human CD4⁺ T Cells

Files in this Data Supplement:

• Supplementary Movie 1 (QuickTime Movie, 1,862 KB) - ζ-GFP-expressing Jurkat T cell interactions with mature DC loaded with 1 μg/ml SEE. Images were acquired every 2 min for 4 h 16 min. Asterisks indicate the T cell at different time points during the movie. White arrows point to the site of initial and persistent ζ clustering. This video corresponds to the still images shown in Fig. 5A.
  
  
• Supplementary Movie 2 (QuickTime Movie, 1,722 KB) - ζ-GFP-expressing Jurkat T cell interactions with immature DC loaded with 1 μg/ml SEE. Images were acquired every 2 min for 6 h 14 min. Asterisks indicate the T cell at different time points during the movie. White arrows point to visible transient ζ clusterings. This video corresponds to the upper still images shown in Fig. 5B.
  
  
• Supplementary Movie 3 (QuickTime Movie, 1,355 KB) - ζ-GFP-expressing Jurkat T cell interactions with immature DC loaded with 1 μg/ml SEE. Images were acquired every 2 min for 6 h 06 min. Asterisks indicate the T cell at different time points during the movie. Black arrowheads point to contacts made with the DCs without any visible clustering. This video corresponds to the lower still images shown in Fig. 5B.
  
  
• Supplementary Movie 4 (QuickTime Movie, 2,738 KB) - Blast T cell interactions with mature DC loaded with 1 μg/ml TSST-1. Phase contrast images were acquired every 30 s for 6 h 38 min. Asterisks indicate the T cell at different time points during the movie.
  
  
• Supplementary Movie 5 (QuickTime Movie, 4,814 KB) - Blast T cells interactions with immature DCs loaded with 1 μg/ml TSST-1. Phase contrast images were acquired every 30 s for 7 h. Asterisks indicate the T cells at different time points during the movie. White arrows point to T cells detaching from the DC.