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* University of Pittsburgh Cancer Institute and
Departments of Pathology, Immunology and Otolaryngology, University of Pittsburgh School of Medicine, Pittsburgh, PA; and
Roswell Park Cancer Institute and Department of Immunology, Buffalo, NY 14263
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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-inducible subunits, LMP-2, LMP-7, and LMP-10 (5), is involved in the generation of antigenic peptide fragments. These fragments are then translocated across the endoplasmic reticulum (ER) membrane via the ATP-dependent heterodimeric transporter associated with Ag-processing subunits TAP-1 and TAP-2. Within the ER, the HLA class I H chain is synthesized and associates with 
2-microglobulin (2m) with the assistance of the chaperone proteins (calnexin, calreticulin, and ERp57), which monitor the proper folding of HLA class I molecules. The HLA class I-2m complex then associates with tapasin, which allows the dimeric complex to interact with TAP and ensures proper peptide loading into HLA- I-2m complex. Then, the trimeric HLA class I-2m-peptide complex is transported to the plasma membrane (3). It has been recognized for some time that functional changes, in particular alterations in the ability to process and present Ags to T cells, are associated with DC maturation (1, 2) and with exposure of DC to tumor cells (6, 7, 8). To date, little is known about the molecular mechanisms underlying these functional changes, and specifically about APM component expression in DC (9, 10, 11). Therefore, taking advantage of a panel of recently developed mAbs to the individual APM components (12) and of the quantitative flow cytometry-based method, we measured in this study the expression of the various components in immature DC (iDC) as well as ex vivo-matured DC (mDC) obtained from peripheral blood monocytes of normal donors (ND) and of patients with cancer. In addition, we evaluated the effects of tumor cells on expression of APM components in DC and their functional potential.
Our data establish for the first time a molecular basis for functional changes that occur during DC maturation. These experiments are also relevant to current DC-based vaccine clinical trials in patients with cancer. The use of autologous ex vivo-activated DC for immunotherapy of cancer is based on the rationale that cross-presentation of tumor-associated Ags (TAA) by DC leads to the generation in vivo of effective TAA-specific immune responses. However, endogenous DC in tumor-bearing animals (6, 7) as well as in patients with cancer (8, 13) have been observed to contain various maturational and functional defects. The abnormalities in dendropoiesis reported for DCs in patients with cancer (14) and evidence that coincubation of DCs with tumor cells induces DC apoptosis (15) suggest that the fate of these APCs may be impaired in the tumor microenvironment. Collectively, these observations point to a dysfunction of DCs in tumor-bearing hosts and indicate that the adoptive transfer of ex vivo-activated DC might correct the existing deficiencies in TAA-specific immune responses and thus be of therapeutic benefit. Our findings show the critical importance of APM for the functional integrity of TAA-specific immune responses and for ex vivo generation of DCs for therapeutic transfers to patients with cancer.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Buffy coats obtained from platelet donors at the Central Blood Bank of Pittsburgh (Pittsburgh, PA) were used as a source of normal monocytes for DC generation. Heparinized peripheral blood was obtained from consented patients with head and neck cancer (HNC), melanoma, or cutaneous T cell lymphoma for DC generation. Peripheral blood or buffy coats were centrifuged on Ficoll-Hypaque (Sigma-Aldrich, St. Louis, MO) gradients. Monocytes were obtained by adherence to plastic as previously described (16) and incubated in AIM V medium (Invitrogen Life Technologies, Carlsbad, CA) containing GM-CSF (1000 U/ml) and IL-4 (10 ng/ml). Cells were harvested on day 6, examined for viability, and analyzed for expression of CD3, CD14, CD19, CD40, CD80, CD83, CD86, and HLA-DR Ags by flow cytometry. For maturation, DC were incubated in medium supplemented with IL-1, IL-6, and TNF-
, all at 10 ng/ml, for 24 h at 37°C. In some experiments, this cytokine mixture also contained PGE2 (10 ng/ml), IFN- (1000 U/ml), IFN- (1000 U/ml), and polyinosinic-polycytidylic acid (poly(IC); 20 µg/ml; Sigma-Aldrich). DC maturation was monitored by measuring the expression of CD40, CD80, CD83, and CCR7 by flow cytometry. iDC and mDC were harvested and used for staining with the anti-APM component-specific mAbs.
Antibodies
mAbs to individual APM components were generated and characterized according to the methodology we previously described (12). The designations and specificity of the mAbs used for staining of DC and their sources are listed in Table I. mAb W6/32 was produced by Dr. A. DeLeo (University of Pittsburgh, Pittsburgh, PA) using a hybridoma obtained from American Type Culture Collection (Manassas, VA). Labeled mAbs for staining of surface Ags on lymphocytes (CD3, CD19, monocytes (CD14) or DC (anti-HLA-DR, -CD80, -CD83, -CD86, -CD11c, -CD40, -CD25) by flow cytometry and anti-CCCR7 mAbs were purchased from Beckman Coulter (La Brea, CA). FITC-conjugated goat anti-mouse IgG Abs were purchased from Caltag Laboratories (Burlingame, CA). Anti-IFN- Abs (1-D1K and 7-B6-1) for use in ELISPOT assays were purchased from MABTECH (Mariemont, OH).
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The squamous cell carcinoma of the head and neck cell lines PCI-4B and PCI-38, were established in our laboratory from a lymph node metastasis and from a primary tumor, respectively, as previously described (17). The melanoma cell line Mel 526 was obtained from Dr. W. J. Storkus (University of Pittsburgh). The cell lines were cultured to confluence in RPMI 1640 supplemented with 10% (v/v) FCS and antibiotics (all from Invitrogen Life Technologies).
Cytokines
IL-1, IL-4, IL-6, and TNF- were purchased from R&D Systems (Minneapolis, MN); IFN- from Schering (Kenilworth, NJ); IFN- from Intermune (Brisbane, CA). GM-CSF was purchased from Immunex (Seattle, WA).
Staining methods
Intracellular staining of DC with the APM component-specific mAbs was performed as previously described (16) with the following modifications. Cells were washed in PBS containing 1% (w/v) BSA and placed in tubes (2–5 x 105 cells/tube). Cells were fixed with 2% (w/v) paraformaldehyde for 20 min at room temperature, washed extensively in PBS-BSA, resuspended in 10 ml of PBS-BSA buffer, transferred into Pyrex flasks (Corning Glass, Corning, NY), and subjected to the microwave treatment for 60 s at low power (<700 W). Cells were then chilled on ice for 10 min, washed, and permeabilized, using 0.1% (w/v) saponin in the PBS-BSA buffer and incubated with the primary Abs (at 10–25 µg/ml saponin-containing buffer) for 30 min at room temperature. After being washed with three changes of the saponin buffer, cells were incubated with optimally pretitered goat anti-mouse IgG Abs for 30 min at room temperature. Cells were then washed in saponin buffer, fixed with 1% (w/v) paraformaldehyde, and analyzed in a Coulter EPICS XL-MCLDC flow cytometer (Beckman Coulter, Miami, FL).
For immunocytochemistry, cells were cytocentrifuged onto positively charged glass slides in a Shandon cytocentrifuge (Shandon, Pittsburgh, PA), fixed in acetone-methanol (1:1), and air dried. Next, the cytospun cells were rehydrated in PBS buffer and blocked with the protein-blocking solution (Shandon). Slides were then incubated with the primary Abs for 1 h at room temperature. After extensive washing, slides were incubated with LSAB-2 reagents (Dako, Carpinteria, CA) according to the directions provided by the manufacturer. Color was developed with diaminobenzidine chromogen (Vector Laboratories, Burlingame, CA), and the slides were counterstained with Gills hematoxylin (Vector Laboratories).
Flow cytometry
Stained cells were examined in an EPICS XL-MCLDC instrument. Two approaches were used: 1) to determine the percentages of stained cells, gate was set on DC, and the number of stained cells vs that of total cells in the gate was determined for each mAb; 2) to quantitate the expression level of each protein, molecular equivalents of soluble fluorochrome (MESF) units were determined, using a standard curve generated by the use of a mixture of four standard beads of known fluorescence intensity and unlabeled blank beads contained in the Quantum Kit (Bangs Laboratories, Fishers, IN). Mean fluorescence intensity of each sample was transformed into MESF units, using the calibration curve calculated by the Quick Cal program supplied by Bangs Laboratories. The fluorescence from the beads was calibrated in terms of MESF each time the experiment was performed.
Coincubation of DC with tumor cell lines
Human iDC or mDC generated from PBMC of ND were cocultured for 24 h at 37°C with tumor cells in a Transwell plate (Costar, Cambridge, MA), plating DC in the bottom compartment and tumor cells in the upper compartment at a 1:1 ratio. Control cultures contained iDC or mDC alone. At the end of a 24-h incubation at 37°C, DC were harvested and used for experiments.
Melanoma Ag (MA)-specific cytolytic T cell generation
The DC coincubated with tumor cells or control DC were pulsed with a Mel526 cell lysate (20 µg protein per ml) for 12–18 h at 37°C, matured in the presence of a cytokine mixture, as described above, and then coincubated with autologous PBMC for 7 or 14 days at 37°C to generate MA-specific T cells by in vitro sensitization (IVS).
ELISPOT assays
The IVS-generated T cells (responders) were harvested and immediately plated in wells of ELISPOT plates (Millipore; Fisher Scientific, Hampton, NH) at a concentration of 1 x 105 cells/well. Mel526 cells (stimulators) were added (1 x 103 cells/well), and the plates were incubated for 24 h at 37°C. The ELISPOT assay for IFN- was performed as previously described (18).
In some experiments, the Mel526-specific CTL line originally obtained from Dr. S. Rosenberg (Surgery Branch, National Cancer Institute, Bethesda, MD) and maintained in our laboratory (18) was used as responder cells to measure the ability of different DC (HLA-A2+) pulsed with Mel526 cell lysates or gp100209–217 peptide to induce IFN- production. The specificity of this T cell line for Mel526 cells was previously tested by us in ELISPOT assays (18).
Preparation of cell lysate
Mel526 cells were harvested using trypsin buffer. After two washes with cold 1x PBS, the cell pellet was then resuspended in lysis buffer (20 mM HEPES buffer, 50 mM NaCl, 10 mM KCl, 1 mM EDTA, 200 mM sucrose, pH 7. 4) at a concentration of 5 x 106 cells/250 µl of buffer. Mel526 cells were then subjected to repeated (3x) freezing at –80°C and thawing at 37°C. After the last thaw, cells were sonicated for 10 s/ml of lysate and centrifuged at 14,000 rpm for 20 min at 4°C. Supernatant was then collected, and the protein concentration was determined, using a Bio-Rad protein assay (Hercules, CA). The cell lysate was then aliquoted into vials and stored at –80°C.
Statistical analysis
Results were analyzed using a paired Student t test. p < 0.05 was considered as significant. The permutation test was used to determine significant differences between triplicate control wells vs experimental wells in ELISPOT assays.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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iDC generated from monocytes obtained from ND or patients with cancer were evaluated for purity by manual cell counts and by flow cytometry. These preparations routinely contained >80% of cells with DC morphology and had the following phenotype (percent range for DC obtained from 12 ND): HLA-DR+ (80–99%); CD86+ (80–99%); CD80 (20–25%); CD83+ (1–15%); CD11c+ (80–99%); CD40+ (50–80%); CD14+ (0–5%); CD3+ (0–10%); CD19+ (0–5%). On maturation, the phenotype of DC was: HLA-DR+ (99–100%); CD86+ (90–99%); CD80+ (80–95%); CD83+ (77–96%); CD40 (70–90%); CD25 (20–45%); and CCR7+ (30–60%). The DC preparations generated from patients with cancer had similar phenotypic characteristics. To be considered as mDC, the cells had to express >70% CD80, >70% CD83, and >30% CCR7.
Verification of the permeabilization and staining procedures
The usual procedure for permeabilization of mononuclear cells involves incubation of cells in the presence of saponin. However, in recent studies (12), microwave treatment has been shown to be the most effective method for staining lymphoid and melanoma cell lines with APM component-specific mAbs. Therefore, in preliminary experiments, we first compared the saponin-based and the microwave methods to ensure that the microwave treatment of DC performed under the conditions described in Materials and Methods was optimal for mAb access to subcellular compartments without interfering with the integrity of the APM components and/or DC morphology. The comparisons were performed independently in two laboratories (by T.L.W. and M.S.). The results presented in Table II show that the microwave treatment was better than saponin permeabilization for staining of DC with APM component-specific mAbs, given that the percentage of stained cells was consistently higher in microwave-treated DC than in saponin-permeabilized DC in the three experiments performed. Also, the procedure performed in parallel in the two laboratories was reproducible, because it yielded similar expression results in terms of the percentage of stained cells. In addition, immunocytochemistry on cytocentrifuge smears showed that the morphology of DC as well as staining intensity and percentages of stained DC were not compromised by the microwave treatment (Fig. 1).
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DC were generated from monocytes obtained from eight ND and stained with mAbs specific for APM components, HLA class I Ags, 2m, and HLA class II Ags. Table III shows that expression of most of the APM components was up-regulated in DC matured in the presence of cytokines. Table III lists only those APM components that were substantially and consistently increased in the percent of stained cells in mDC preparations relative to iDC preparations. Two additional points are noteworthy. A considerable variability was observed among ND in the expression of APM components by DC, as indicated by a broad range of the percent stained cells (Table III). Furthermore, HLA class I Ags, 2m, and HLA class II Ags were expressed in almost 100% of both iDC and mDC.
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2m, and HLA class II Ag expression was higher in mDC than in iDC (Tables IV and V). In addition, immunocytochemistry performed on smears of iDC and mDC confirmed that the expression of APM components was higher in mDC than in iDC, as illustrated for TAP-1 in Fig. 2.
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, TNF-, IFN-, IFN-, and poly(IC). These mDC preparations (mDC4) contained >90% CD80+, > 90% CD83+, and >50% CCR7+ cells (Table VI).
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ELISPOT assays were performed with effector T cells generated by IVS as responders and Mel526 cells as stimulators. T cells coincubated with mDC consistently gave a significantly higher number of spots than T cells coincubated with iDC (Fig. 3). In addition, effector T cells generated with iDC spontaneously released IFN-, giving a very high background in ELISPOT assays, whereas T cells primed with mDC gave only few background spots (Fig. 3). Thus, priming only with mDC resulted in the generation of MA-specific effector cells. These results are compatible with the hypothesis that the DC maturation process, which includes up-regulation of the APM components, is of key importance for subsequent productive interactions of mDC with T cells.
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production in MA-specific CTL. In contrast, pulsing of iDC with the gp100209–217 peptide followed by maturation resulted in only modest up-regulation of APM component expression, and these DC mediated a lower response than DC pulsed with the melanoma cell lysate in ELISPOT assays (Fig. 4). In aggregate, these data suggest that up-regulated APM component expression correlates with mDC abilities to prime precursor T cells as well as present MA epitopes to MA-specific CTL.
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We next asked whether APM component expression in iDC and mDC generated from the patients’ PBMC was comparable with that seen in iDC and mDC generated from ND’s PBMC. Comparison of the fluorescence intensity (in MESF units) for all individual APM components showed similar expression in iDC obtained from patients with cancer and ND (Fig. 5). Changes in APM component expression during maturation of DC obtained from patients with cancer were also measured and found to be up-regulated in mDC vs iDC. The largest maturation-related increases were observed in the expression of Delta, LMP-2, TAP-1, calnexin, and HLA class I Ags (Fig. 5, A–D). However, these increases were modest compared with those seen with DC of ND (Fig. 5E). Furthermore, in some patients with metastatic melanoma, monocyte-derived DC failed to up-regulate APM component expression on exposure of iDC to the cytokine maturation mixture for 18–24 h. When pulsed with a gp100209–217 peptide and used as stimulators in ELISPOT assays, these DC gave 1 log fewer spots than similarly matured and pulsed DC obtained from ND (data not shown).
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To study the effects of tumor cells on APM component expression in DC, iDC obtained from 6 ND were coincubated with HNC tumor cell lines (PCI-4B and PCI-38) in Transwell plates. At the end of a 24-h incubation at 37°C, DC were tested for APM component expression. As shown in Table VII, percentages of DC expressing MB1, LMP-2, LMP-7, LMP-10, and ERp57 proteins were consistently decreased on coincubation with tumor cells. In contrast, no significant change was detected in the percentage of DC expressing Delta, calnexin, calreticulin, and tapasin. HLA class I and class II Ag expression was also reduced in iDC incubated with tumor cells (data not shown).
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Functional defects in DC after coincubation with tumor cells
Additional experiments tested whether APM component down-regulation observed in DC coincubated with tumor cells was associated with an impairment of their Ag-processing capability. To this end, iDC were incubated with a tumor cell supernatant for 18 h, pulsed with a Mel526 cell lysate, and matured in the presence of cytokines. Control iDC, which had not been incubated with tumor cell supernatant, were pulsed with the same Mel526 cell lysate, incubated in medium alone, and matured. The mDC were then cocultured with autologous PBMC for 7 or 14 days. T cells generated in the control or experimental cocultures were tested in ELISPOT assays for IFN- production in the presence of Mel526 cells as stimulators. As shown in Fig. 6, when DC exposed to tumor cell supernatants were used for IVS, they generated T cells which were unresponsive to Mel526 cells. In contrast, DC not treated with tumor cell supernatants induced T cells that responded to Mel526 targets by IFN- production, giving a high number of spots. This response was HLA class I-restricted, because it was inhibited by mAb W6/32 (Fig. 6).
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Although adoptive transfers of DC in the form of vaccines are being used with increasing frequency in patients with cancer, to the best of our knowledge, no information is available about expression of individual APM components in DC used for therapy. Although it has been acknowledged that the maturation step in a mix of cytokines is important for the quality of DC-driven immune responses (19), the molecular changes associated with these functional phenomena have remained unknown. We have demonstrated that DC maturation allows for a better expression of APM components in these cells. Thus, mDC were shown to express higher levels of APM components and in a greater proportion of cells than iDC. In parallel in vitro experiments, these mDC were shown to generate superior TAA-specific T cell responses, as measured in ELISPOT assays.
The second DC-related issue in the therapeutic use of autologous antitumor vaccines concerns the quality of DC generated ex vivo from monocytes obtained from patients with cancer. In view of reports in the literature describing defective dendropoiesis or tumor-induced apoptosis of DC in patients with cancer (14, 15), this is a legitimate concern. For this reason, we compared expression of APM components in iDC and mDC generated ex vivo from monocytes of patients with various malignancies and found that it is suppressed relative to that seen in DC of ND. APM component expression was generally not up-regulated during maturation in cytokines to the levels observed in mDC obtained from ND. In some patients with melanoma, it was not up-regulated at all, suggesting a need for a stronger maturation signal. This is an important observation, which focuses attention on the composition of the cytokine mix used for ex vivo DC maturation. As recently shown by Kalinski and colleagues (19), ex vivo polarization of DC in appropriately selected mix of cytokines determines the quality of T cell responses induced ex vivo and, presumably, in vivo. The addition of IFN-, IFN-, and poly(IC) to the conventional maturation mixture (20) appears to induce higher expression of APM components in DC of patients with cancer.
Finally, we investigated ex vivo effects of tumor cells on APM component expression in human DC and on the functional potential of the DC to generate TAA-specific effector T cells. Dysfunction of DC, including defects in Ag processing/presentation, might provide tumor cells with a mechanism of escape from the host’s immune system. For example, down-regulation of proteins responsible for processing and/or presentation of TAA could impair the generation of TAA-specific immune responses (21, 22). Although various tumor cells probably have the ability to process Ags, it has now been accepted that DC are largely responsible for processing and cross-presentation to T cells of TA internalized in the form of apoptotic/necrotic tumor cells or Ag-Ab complexes (23).
Using tumor cell lines established by us and previously shown to exert inhibitory ex vivo effects on human DC (PCI-4B and PCI-38; our unpublished data), we demonstrated that in a Transwell system, these tumor cells down-regulated expression of several APM components in iDC. In addition, functions of these DC coincubated with tumor cells were impaired. When DC coincubated with tumor cells were used for ex vivo generation of TA-specific T cells, the cultures contained a significantly lower frequency of such T cells than the control cultures. These results show that human tumor cells or their supernatants have profound effects on the Ag-processing function of DC and on their ability to prime T cells. It is likely that similar mechanisms operate in vivo. Therefore, adoptive transfers of DC to patients with cancer should be combined with cytokines able to up-regulate expression of APM components in DC and to protect these APC from tumor-induced dysfunction.
The data reported here provide important insights into the crucial significance of the Ag-processing pathway in human DC for the generation and maintenance of TAA-specific immune responses. They also indicate that tumors can exert strongly inhibitory effects on DC and suggest that in the context of biotherapies with DC, it is necessary to focus attention on the quality of Ag-processing functions in adoptively transferred DC. Lastly, the association between APM component down-regulation and functional defects of DC suggests that these components could serve as molecular markers to monitor the quality of DC preparations used in active specific immunotherapy of patients with cancer. If additional studies in a large number of patients show an association between APM component down-regulation in DC and defects in the induction of TAA-specific immune responses, monitoring of APM component expression in DC preparations may be used for evaluation of their functional quality.
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Footnotes |
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1 This work was supported by U.S. Public Health Service Grants CA-67108 (to S.F.), CA-82016 (to T.L.W.), DE-13918 (to T.L.W.), and DE-12321 (to T.L.W.) from the National Institutes of Health. 
2 Address correspondence and reprint requests to Dr. Theresa L. Whiteside, University of Pittsburgh Cancer Institute Research Pavilion at the Hillman Cancer Center, 5117 Centre Avenue, Suite 1.27, Pittsburgh, PA 15213-1863. E-mail address: whitesidetl{at}msx.upmc.edu
3 Abbreviations used in this paper: DC, dendritic cell; iDC, immature DC; mDC, mature DC; APM, Ag-processing machinery; HNC, head and neck cancer; TAA, tumor-associated Ag; ER, endoplasmic reticulum; 2m, 2-microglobulin; MESF, molecular equivalents of soluble fluorochrome; IVS, in vitro sensitization; poly(IC), polyinosinic-polycytidylic acid.
Received for publication January 14, 2004. Accepted for publication May 10, 2004.
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References |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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-mediated coordinated transcriptional regulation of the human TAP1 and LMP2 genes in human renal cell carcinoma. Clin. Cancer Res. 3:573.[Abstract]
proteasome regulator but little increase in the steady-state content of immunoproteaseome during dendritic cell maturation. Eur. J. Immunol. 31:3271.[Medline]
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