Chemoattractant Signals and ₂ Integrin Occupancy at Apical Endothelial Contacts Combine with Shear Stress Signals to Promote Transendothelial Neutrophil Migration

Files in this Data Supplement:

• Figure S1 (PDF, 165 KB) - Fig. S1 depicts ultra-thin cross sections of neutrophils interacting with PAF-presenting TNF-α-activated HUVEC under shear stress conditions as shown in Fig. 8A. Bar = 1μm.
• Video 1 (QuickTime, 2,492 KB)
• Video 2 (QuickTime, 2,495 KB) - Videos 1 and 2 depict time lapse recordings of neutrophils interacting with PAF-presenting non-activated HUVEC under shear-free and shear stress conditions, respectively, as described in Fig. 1.
• Video 3 (QuickTime, 2,227 KB) - Video 3 depicts a neutrophil (in the middle of the field) locomoting towards the endothelial border. Note the sudden conversion of the rear of this neutrophil into a leading edge and the transmigration of this new edge right through an endothelial cell (EC) body.
• Video 4 (QuickTime, 2,944 KB) - Video 4 shows neutrophil TEM through a singular EC within a non-confluent monolayer. Videos depict field segments recorded at 1 frame/sec.
• Figure S2 (TIF, 589 KB) - Fig. S2 depicts one frame of video 4 in which the EC border and nucleus are marked; the arrow indicates an apically adherent neutrophil about to transmigrate through the endothelial cell. The time (in min) elapsed in the different videos is shown at the top.