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* National Cancer Institute, National Institutes of Health, Bethesda, MD 20892;
Howard Hughes Medical Institute-National Institutes of Health, Research Scholars Program, Bethesda, MD 20815; and
Department of Biochemistry and Molecular Biology, The George Washington University School of Medicine and Health Sciences, Washington, D.C. 20037
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Abstract |
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, release of perforin, and activation of caspase-3 in target cells, was confined to Ag-expressing tumor. We thus conclude that CD8+ T cell-mediated destruction of tumor is the result of specific T cell triggering at the tumor site. The ability to induce ubiquitous homing and specific tumor destruction may be important in the case of noninflammatory metastatic tumor foci. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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4
7 integrin is reportedly involved in T cell homing to the gut (5), but it has been difficult to show such specificity for homing to other peripheral tissues (6, 7). The homing of Ag-experienced lymphocytes may initially be nonspecific, because they forage for their cognate Ag. When T cells come into contact with their specific Ag, they may be retained in Ag-containing tissue (8). Adoptive transfer of activated T cells, followed by specific stimulation, can result in in vivo destruction of tumor in mice and humans (9, 10), but how these cells infiltrate tumor tissue remains unknown. We have previously analyzed the trafficking of antitumor T cells in patients with melanoma using 111In-labeled lymphocytes and whole-body gamma camera imaging (2). We found 111In in liver, spleen, and lungs as well as in metastatic deposits, but it could not be determined whether live 111In-labeled T cells specifically trafficked to these locations, or the 111In nuclide had lodged at these locations as a result of the death, phagocytosis, or target specificity of the adoptively transferred T cells (2).
To experimentally examine T cell trafficking into tumors, we used a recently described murine model, termed pmel-1, which expresses a transgenic CD8+ TCR and is reactive against the melanoma/melanocyte Ag, gp10025–33. Adoptive transfer of these cells along with a vaccine encoding the altered peptide ligand and administration of exogenous IL-2 result in the regression of established s.c. B16 melanoma in C57BL/6n mice (9). We examined the role of T cell activation in tumor infiltration and determined the kinetics and specificity of T cell trafficking into tumor that expressed or did not express the target Ag.
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Materials and Methods |
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Pmel-1 TCR, pmel-1/GFP+, pmel-1/Thy1.1+ transgenic mice (9) and C57BL/6n mice were bred and housed at the National Institutes of Health according to the guidelines of the animal care and use committee at National Institutes of Health. Pmel-1/Thy1.1+ transgenic mice were deposited at The Jackson Laboratory (strain 5023; Bar Harbor, ME). Transgenes were confirmed by PCR analysis for the pmel-1 TCR - and -chains, at 100 pM of each of the following: TCR sense, 5'-GGT CCT GTG GCT CCA GTT TAA T-3'; TCR antisense, 5'-CTG CTT AAC CTG TCC CTC ATG T-3'; TCR sense, 5'-CTG GGC AGT GTT CTG TCT CC-3'; TCR antisense, 5'-ACC ATG GTC ATC CAA CAC AG-3'; and gold Taq (Applied Biosystems, Branchburg, NJ), with 1x PCR buffer, 3 mM MgCl, and 10 mM dNTPs. PCR was performed at 94°C for 13 min, then (94°C for 1 min, 61°C for 1 min, 72°C for 1.5 min) for 35 cycles and at 72°C for 15 min. Both (H-2b) B16, a gp100+ spontaneous murine melanoma, and methylcholanthrene 205 (MCA-205)4 were obtained from the National Cancer Institute tumor repository. Tumors were maintained in complete medium (CM) comprised of RPMI 1640 with 10% heat-inactivated FBS (Biofluids, Rockville, MD).
RNA preparation and RT-PCR
B16 and MCA-205 tumors were lysed, and RNA was isolated using the RNeasy Mini kit (Qiagen, Valencia, CA). cDNA was obtained using random hexamer primers and the SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen Life Technologies, Carlsbad, CA). The quantities of gp100 and -actin mRNAs were evaluated by PCR primers using intron-spanning primers (11, 12) at 25, 30, and 35 cycles and digital capture.
In vitro activation and cytokine release
Pmel-1 splenocytes from transgenic mice were cultured for 1 wk as previously described (9). For cytokine release assays, 1 x 105 T cells were cocultured in CM with 1 x 105 B16, MCA-205, or C57BL/6n splenocytes that were pulsed with or without 1 µM human gp10025–33 peptide and washed twice. Supernatants were collected after 24 h and tested using a mouse IFN ELISA kit (Endogen, Rockford, IL).
Adoptive cell transfer, vaccination, and cytokine administration
Pmel-1 transgenics or C57BL/6n mice at 6–12 wk of age were implanted s.c. with 2–5 x 105 B16 melanoma cells and/or 2–5 x 105 MCA-205 colon carcinoma on opposing flanks and in vivo stimulated as described previously (9). Briefly, at the time of T cell transfer, mice (n = 5 for each group) were injected i.v. with 2 x 107 PFU of recombinant fowlpox encoding human gp100 and injected i.p. with 6 x 106 IU of recombinant human IL-2 in PBS (Chiron, Emeryville, CA) twice daily for a total of five or six doses. To induce lymphopenia, C57BL/6n tumor-bearing mice were sublethally irradiated with 5 Gy before adoptive transfer of in vitro-activated pmel-1 splenocytes (1–2 x 106 CD8+V13+ T cells) and in vivo stimulation (9). Mice were randomized, and tumors were measured in a blinded fashion using digital calipers. The products of the perpendicular diameters are presented as the mean ± SEM.
Organ collection and cytofluorometric analysis of adoptively transferred cells
On the days indicated, mice were bled by tail vein into heparin-containing microcentrifuge tubes, killed, and perfused with heparin in HBSS or PBS (1 U/ml; injection of 10–15 ml in the left ventricle of the heart with rupture of the right ventricle), and their organs were harvested and homogenized into a single-cell suspension using the rubber end of a 3-cc syringe and a 40-µm filter cup. Cells were labeled with the following mAbs (BD Pharmingen, San Diego, CA): FITC-conjugated anti-V13 (MR12-3), FITC- or PE-conjugated anti-Thy1.1 (HIS51), PE-conjugated anti-CD44 (IM7), PE-conjugated anti-CD62L (MEL-14), and CyChrome- or allophycocyanin-conjugated anti-CD8 (53-6.7). Samples were analyzed using a FACSCalibur flow cytometer and WinMDI V2.8 software (Dr. J. Trotter; http://facs.scripps.edu). Samples were enumerated using trypan blue exclusion or with a cell counter (Guava Technologies, Hayward, CA). The absolute number of pmel-1 T cells was calculated by multiplying the absolute cell count by the total percentage of pmel-1 T cells. For enumeration of pmel-1 in B16 and MCA-205, tumors of equal size (
20 mm2 in area) were homogenized and stained as previously described. For intracellular staining, tumors were homogenized, then pulsed with 1 µM human gp10025–33 and Golgi-Stop (BD Pharmingen) for 3.5 h, stained extracellularly, permeabilized using the Cytofix/Cytoperm kit (BD Biosciences), and stained with allophycocyanin-conjugated anti-IFN- mAbs (XMG1.2).
BrdU incorporation
Four days after treatment, mice were injected i.p with 1–1.5 mg of BrdU (Sigma-Aldrich, St. Louis, MO) for 2 h, then bled, killed, and perfused. Their organs were harvested, homogenized, and stained extracellularly for pmel-1. Cells were permeabilized using Cytofix/Cytoperm (BD Biosciences), treated with DNase I (Sigma-Aldrich) for 1 h at 37°C, then stained with FITC-, PE-, or allophycocyanin-conjugated anti-BrdU (BD Biosciences), and analyzed using FACS.
Confocal microscopy
Organs were harvested, imbedded in optimal cutting temperature compound (4583; Sakura Finetek, Torrance, CA), frozen at –80°C, and sectioned using a cryostat (Molecular Histology, Gaithersburg, MD). Frozen sections were treated and stained with FITC-conjugated anti-Thy1.1, FITC-conjugated anti-V13, PE-conjugated anti-perforin (H-315; Santa Cruz Biotechnologies, Santa Cruz, CA), and active caspase-3 (C95-605; BD Biosciences) mAbs, then secondarily stained with Texas Red-conjugated, anti-goat, anti-rabbit (sc-2780; Santa Cruz Biotechnology). Images were obtained using a Leica TCS spectrophotometer confocal inverted microscope (NCI Core Fluorescence Imaging Facility, Bethesda, MD).
Statistics
Statistics were determined using factorial or repeated measure ANOVA (StatView statistical software; Abacus Concepts, Berkeley, CA). Differences were considered significant at p < 0.05.
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Results |
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We explored whether T cells with tumor specificity were able to traffic to an Ag-expressing tumor site. We hypothesized that tumor activation of T cell precursors with specificity for tumor-associated Ags could trigger the extravasation and accumulation of T cells in the tumor. To assess whether tumor alone could activate tumor-specific CD8+T cells, we used a TCR-transgenic mouse, called pmel-1, which was developed in our laboratory. Pmel-1-transgenic mice express a TCR specific for the unmutated melanoma/melanocyte-associated Ag, gp100 (9).
We implanted the B16 mouse melanoma on transgenic mice and evaluated the activation of resident antitumor T cells in the spleen. As previously described, unmanipulated pmel-1-transgenic splenocytes maintained a basal (Fig. 1A), but variable (data not shown), level of CD44 and CD62L expression, indicative of a relatively naive phenotype and similar to that in naive C57BL/6n mice. In the data shown in Fig. 1A, these distinctions were pronounced in the tumor-bearing transgenic mice. Pmel-1 T cell expression of CD44 (an activation marker) increased from 32% in unvaccinated mice to 81% in vaccinated transgenic mice. Likewise, the expression of CD62L (which moves in the opposite direction (i.e., lower) with Ag stimulation) on pmel-1 T cells decreased from 60% in unvaccinated mice to 9% in vaccinated mice (9). Interestingly, the presence of the B16 tumor alone did not activate transgenic cells. Pmel-1 T cells could be activated, as defined by up-regulation of CD44 and down-regulation of CD62L, after IL-2 administration and immunization with recombinant fowlpox (rFPVhgp100) encoding an altered form of the target epitope, gp10025–33, as previously described (9).
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13+ T cells in a representative experiment (Fig. 1B). These data are consistent with the hypothesis that the Ag-specific vaccine, but not the B16 tumor, is responsible for the activation of pmel-1 T cells in spleen and their accumulation in tumor. As seen previously, tumor growth was unimpeded in unmanipulated pmel-1-transgenic mice, similar to that in wild-type littermates (9) (Fig. 1C). We extended our earlier findings by showing that pmel-1-transgenic mice that received rFPVhgp100 and IL-2 underwent tumor regression.
From these data it appeared that the presence of tumor alone did not activate naive pmel-1 T cells, nor did naive cells infiltrate into the tumor regardless of their abundance. Conversely, pmel-1 T cells appeared in the tumor in large numbers after activation, and this intensive T cell infiltration correlated with tumor destruction.
Role of target Ag expression in T cell trafficking
We studied the presence of T cells in tumor and specifically addressed what role the target Ag played in T cell migration. We used a tumor, MCA-205, that does not express mRNA for the target Ag, gp100 (Fig. 2A), and is not recognized by activated pmel-1 T cells unless pulsed with gp10025–33 peptide (Fig. 2B). To study the specificity of these cells in vivo, we implanted B16 and MCA-205 in the same mouse, on opposite flanks. We then treated these mice bearing two 7-day-old tumors using adoptive transfer of activated pmel-1 T cells, rFPVhgp100, and IL-2 (9). Adoptively transferred and in vivo-stimulated pmel-1 cells exhibited exquisite specificity for the in vivo destruction of Ag-expressing B16 tumor, but not Ag-negative MCA-205 (Fig. 2C).
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Using confocal microscopy and Ab staining for V13, we directly observed pmel-1 T cell infiltration into both tumors after intravascular flushing 5 days after adoptive transfer and in vivo stimulation (Fig. 3B). There were approximately equal numbers of pmel-1 T cells found in the B16 and MCA-205 tumors (Fig. 3B, lower panels). To more precisely enumerate the cells, we resected tumors of approximately equal size, then harvested and homogenized them into a single-cell suspension (Fig. 3C). Although the difference in the total number of pmel-1 found in tumor in treated vs untreated mice was significant (p < 0.05), there was no significant difference between the total pmel-1 in B16 or MCA-205 (p = 0.52) in treated mice.
It thus appears that in vivo-stimulated pmel-1 T cells infiltrate many organs regardless of whether they express the target Ag. Yet how do the cells achieve such wide distribution? We next determined whether the infiltration of pmel-1 T cell into all tissues was the result of proliferation in these tissues or of proliferation in one tissue and subsequent migration.
Pmel-1 T cells proliferate in multiple tissues
To determine where tumor-specific CD8+ cells divide, we used the thymidine analog BrdU. This reagent is incorporated into the DNA of actively dividing cells and is detectable by FACS using a specific Ab-staining method. Four days after adoptive cell transfer, mice received a 2-h pulse of BrdU given i.p., then were bled and perfused, and organs were harvested and homogenized. Cells were stained for the expression of CD8, V13, and BrdU.
After the administration of vaccine and IL-2, rapidly proliferating pmel-1 cells were found virtually everywhere, in lymph node, spleen, liver, and lung, but to a lesser extent in blood and kidney (Fig. 4A). Pmel-1 T cells transferred alone did not exhibit a profound proliferation and were not found in kidney (not shown). In a similar experiment, activated pmel-1 proliferated similarly in the Ag-expressing B16 and in the Ag-null MCA-205 (Fig. 4B). Our findings suggest that these highly activated CD8+ tumor/self-reactive T cells not only infiltrate nonspecifically, but also proliferate indiscriminately in multiple organs after stimulation. We explored why trafficking and proliferation were ubiquitous, but tumor destruction was specific.
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To investigate the requirements for specific tumor destruction, we assessed intracellular IFN- production by pmel-1 T cells in gp100+ and gp100– tumors in vivo. We found that pmel-1 T cells in the Ag-positive B16 tumor, but not those in the Ag-null MCA-205 tumor (Fig. 5A) or in spleen and lymph node (data not shown), up-regulate intracellular IFN-. IFN- is important in viral clearance and MHC up-regulation on target tissue (16), but may not be indicative of T cell-mediated tissue destruction. We chose to examine perforin release, which is more directly associated with T cell-mediated tissue destruction.
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13+ pmel-1 cells in both tumors, but we only saw perforin staining in B16, not MCA-205, tumor (Fig. 5B) or spleen (data not shown). The downstream effect of perforin and granzymes includes the activation of caspases, in particular caspase 3 (18). With the transfer of in vivo-stimulated pmel-1, we observed staining of active caspase 3 in B16 (cover), but not MCA-205 (Fig. 5C) or spleen (data not shown). B16 from untreated mice did not stain for active caspase 3. |
Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Although the egress of activated CD8+ T cells into the tumor is not Ag specific (7), this infiltration may be required for tumor destruction (2, 3). Thus, workers have attempted to enhance T cell migration to the tumor. In an effort to circumvent the need for trafficking, direct intra-arterial infusion of T cells into the tumor can be successful (19), but is impractical in the case of widely metastatic disease. In nonaccessible tumor sites, induced expression of adhesion molecules, such as chemokine receptors, integrins, or selectins, may enhance T cell migration to the periphery (20, 21, 22). The induction of CXCR2 or CXCR4 into T cells may augment T cell targeting to melanoma or leukemias derived from marrow stromal cells that express their respective ligands, CXCL1 or stromal cell-derived factor-1 (23, 24). Other approaches include modulation of the tumor environment to induce T cell infiltration (25). However, tumor-reactive T cell migration to the tumor alone does not reliably result in tumor regression (26, 27, 28). This may be due to the tumor’s inability to efficiently activate tumor-reactive T cells (29, 30, 31, 32), although this may not be the case in all tumors (33). Thus, a potent T cell activation may be required to mount an effective immune response (14, 34).
Exogenous cytokine administration and, in some cases, Ag in the context of danger signals have been shown to stimulate adoptively transferred tumor-reactive T cells and enable them to induce tumor regression (1, 9, 28, 35). Transfer of these cells into a lymphopenic environment may accentuate these results (our unpublished observations) (10). The mechanisms involved in this tumor destruction have not been clearly delineated. Although it has been demonstrated that secondary stimulation of CD8+ T cells results in rapid proliferation, a peak, and subsequent contraction in lymphoid organs (15), little has been shown in peripheral organs. In the current study we found that in vivo stimulation of tumor-reactive T cells not only induces similar kinetics in spleen, blood, and lymph nodes, but also extends into multiple peripheral tissues. This rapid kinetic curve appears to occur simultaneously in all tissues, countering the idea of lymphoid proliferation and then peripheral migration. In addition, we found that several days after stimulation, tumor-reactive T cells were actively dividing in both Ag-positive and negative tumors. This phenomenon is in congruence with several recent studies demonstrating T cell programming and proliferation after a single antigenic exposure (36, 37). It is interesting to note that active T cell proliferation in the blood was not observed; perhaps some property of or in the peripheral tissues, but not the blood, is necessary for induction into S phase.
Although the migration and proliferation of these in vivo stimulated T cells are not tissue specific, tumor recognition and destruction are specific. IFN- is a reliable indicator of CD8+ T cell-specific target recognition (38, 39) that induces the up-regulation of MHC I on B16 (16). Furthermore, cytolytic molecules, such as perforin, can be observed in B16, but not MCA-205, treated with in vivo-stimulated pmel-1 T cells. The release of perforin in conjunction with granzymes by tumor-reactive T cells induces an apoptotic cascade in target tumor (40), mediating tumor cell death. This tumor killing is visualized by the staining of active caspase 3 in B16 infiltrated with pmel-1 T cells, but not in MCA-205, even in the presence of pmel-1 T cells.
The ability to raise large numbers of tumor-reactive T cells and induce ubiquitous T cell migration and the specific killing of tumor cells by T cells may have important clinical implications. The current vaccination strategies available can induce large quantities of tumor Ag-specific T cells, but these vaccinations do not reliably induce tumor regression (41). An alternative strategy, based on adoptive T cell transfer, involves the ex vivo expansion of large quantities of tumor-reactive T cells. This methodology obviates the requirement for continuous in vivo vaccination and enables the possibility of pretransfer lymphodepletion, which may enhance T cell-based immunotherapies. Furthermore, an increase in cell number simplifies the logistics involved in genetic modification of T cells with TCRs against tumor Ags (42) or with homing markers (23, 24).
Tumor-homing strategies are currently being explored in our laboratory and elsewhere, but the molecular bases of directing T cells specifically to tumor metastases remain largely unknown. As we have reported in the present manuscript, the in vivo stimulation of adoptively transferred cell circumvents the need for specific targeting, because cells migrate indiscriminately and ubiquitously. In the case of metastatic disease, tumor can infiltrate multiple organ systems. The ability to induce ubiquitous T cell infiltration, while maintaining specific-tumor killing, may be beneficial in the treatment of metastatic, noninflammatory tumor foci.
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Acknowledgments |
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Footnotes |
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1 This study was performed in partial fulfillment of a Ph.D. in Biochemistry (to D.C.P.) at The George Washington University, Washington, D.C. 
2 Address correspondence and reprint requests to Douglas C. Palmer, National Cancer Institute, National Institutes of Health, Clinical Research Center, Room 3W 35816, Bethesda, MD 20892-1201. E-mail address: palmerd{at}mail.nih.gov
3 D.C.P. and S.B. contributed equally to this work.
4 Abbreviations used in this paper: MCA, methylcholanthrene; CM, complete medium; rFPV, recombinant fowlpox.
Received for publication June 8, 2004. Accepted for publication October 11, 2004.
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References |
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-actin transposed into a BAM5 sequence: insertion has created 13 base-pair direct repeats. Nucleic Acids Res. 13:3031.-facilitated rejection of murine B16 melanomas. J. Immunol. 161:897.. Eur. J. Immunol. 32:2117.[Medline]
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