Files in this Data Supplement:

• Movie 1 (Microsoft AVI Video, 2,057 KB) - SDF-1 induces cellular deformations in primary CD4+ T cells.
  CD4+ T cells nucleoporated with GFP-actin were plated in HBSS buffer supplemented with 0.5% FCS on poly-lysine-coated glass coverslips mounted on Petri dishes (MatTek, Ashland, MA). Acquisition of a Z-stack (1 μm sections) was performed at 37°C every 10 s during 17 min on a spinning-disk confocal microscope. SDF-1 (30 nM) was added when indicated. A projection image of the stack is shown.
• Movie 2 (Microsoft AVI Video, 2,358 KB) - gp120 induces cellular deformations in primary CD4+ T cells.
  GFP-actin-expressing CD4+ T cells were plated on poly-lysine-coated glass coverslips mounted on Petri dishes. Acquisition of a Z-stack (1 μm sections) was performed at 37°C every 10 s during 20 min on a spinning-disk confocal microscope. Gp120 (200 nM) was added when indicated. A projection image of the stack is shown.