Files in this Data Supplement:

• Materials and Methods (Microsoft Word, 70 KB)
• Table I (Microsoft Excel, 35 KB) - The abbreviation, full name, manufacturer, source, catalog number, dosage, and the stock solution protocol of single ligands used in B cell single ligand screen.
• Table II (Microsoft Excel, 11,754 KB) - The annotation table of the Agilent cDNA array.
• Table III (Microsoft Excel, 7,176 KB) - Log₂(Treated/0 h) values for 2937 differentially expressed features identified by SAM. The log₂(Treated/0 h) value was calculated by subtracting log₂(0 h/Spleen) from log₂(Treated/Spleen), where the log₂(Treated/Spleen) is the average of at least two out of three replicate log₂(Cy5/Cy3) measurements of cultured B cells and log₂(0 h/Spleen) the average of at least 6 out of the 26 replicate log₂(Cy5/Cy3) measurements of 0h B cells. For features that did not have at least two replicate measurements in cultured B cells or at least 6 replicate measurements in 0 h B cells, their log₂(Treated/0 h) value was set to blank
• Table IVa (Microsoft Excel, 9,889 KB)
• Table IVb (Microsoft Excel, 10,983 KB)
• Table IVc (Microsoft Excel, 10,955 KB)
• Table IVd (Microsoft Excel, 8,798 KB)
• Table IVe (Microsoft Excel, 3,332 KB) - Log₂(Treated/0 h) values for all array features.
  1. The log₂(Treated/0 h) values of B cells cultured with media (UNTR), 2MA, Anti-Ig, BAFF, BLC, BOM, CD27, CD40L.
  2. The log₂(Treated/0 h) values of B cells cultured with CGS, CpG, DIM, ELC, fMLP, IFNβ, IFNγ.
  3. The log₂(Treated/0 h) values of B cells cultured with IGF-1, IL-10, IL-4, LPA, LPS, LTB4, MIP3α, NEB.
  4. The log₂(Treated/0h) values of B cells cultured with NGFβ, NPY, PAF, PGE, SIP, SDF1α, SLC, TER.
  5. The log₂(Treated/0 h) values of B cells cultured with TGFβ and TNFα. The log₂(Treated/0 h) value was calculated by subtracting log₂(0 h/Spleen) from log₂(Treated/Spleen), where the log₂(Treated/Spleen) is the average of at least two out of three replicate log₂(Cy5/Cy3) measurements of cultured B cells and log₂(0 h/Spleen) the average of at least 6 out of the 26 replicate log₂(Cy5/Cy3) measurements of 0 h B cells. For features that did not have at least two replicate measurements in cultured B cells or at least 6 replicate measurements in 0 h B cells, their log₂(Treated/0 h) value was set to blank
• Table V (Microsoft Excel, 369 KB) - Expression fold-changes and GO designations of features showing similar temporal patterns of expression changes in response to Anti-Ig, CD40L, CpG, IL-4 and LPS. Only features changed expression at least 30% to each ligand were included. Features are listed in ascending order of their featureNumbers. The probe name, gene symbol, LocusLink, expression fold-changes¹ in response to Anti-Ig, CD40L, CpG, IL-4 and LPS, as well as the Go term designations of the features are included in the excel spreadsheets. Sheet 1 lists the features that increased expression with time; sheet 2 lists features that decreased expression with time; sheet 3 lists the features whose functions are related to protein synthesis
• Table VI (Microsoft Excel, 47 KB) - Expression fold-changes and GO designations of features of known genes showing unique expression patterns in response to IL-4. Features are listed in ascending order of their feature numbers. The probe name, gene symbol, LocusLink, expression fold-changes¹ in response to Anti-Ig, CD40L, CpG, IL-4 and LPS, as well as the Go term designations of the features are included.
• Table VII (Microsoft Excel, 91 KB) - Expression fold-changes and GO designations of features of known genes showing unique expression patterns in response to Anti-Ig. Features are listed in ascending order of their feature numbers. The probe name, gene symbol, LocusLink, expression fold-changes¹ in response to Anti-Ig, CD40L, CpG, IL-4 and LPS, as well as the Go term designations of the features are included.
• Table VIII (Microsoft Excel, 31 KB) - Expression fold-changes and GO designations of features of known genes showing unique expression patterns in response to CD40L. Features are listed in ascending order of their feature numbers. The probe name, gene symbol, LocusLink, expression fold-changes¹ in response to Anti-Ig, CD40L, CpG, IL-4 and LPS, as well as the Go term designations of the features are included.
  
  Reference
  1. Pfaffl, M. W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45.