Files in this Data Supplement:

• Video 1 (QuickTime, 1,038 KB)
• Video 2 (QuickTime, 3,543 KB) -
  Video 1 and 2: Time-lapse movie of 2 day old Lm-infected PMN. Freshly isolated PMN were co-incubated with Lm promastigotes at a 1:5 ratio for 3 hours. Extracellular parasites were removed. 42 h after infection time-lapse movies were made. For video 1, infected PMN were adhered to object slides on a 1% poly-L lysine (Sigma-Aldrich, Germany) coating. Time-lapse video1 was recorded 1 frame per 5 sec and played back 10 frames per sec (time lapse factor is 50). For video 2 we used uncoated object slides and recorded 5 frames per sec and played back 20 frames per sec (time lapse factor is 4). In both videos, intracellular Leishmania are highly motile and the flagella are clearly visible.
• Video 3 (QuickTime, 3,963 KB) - Video 3: Time-lapse movie of a macrophage phagocytosing an infected PMN. Co incubation as described in Fig 3C, time-lapse video 3 was recorded 1 frames per 3 sec and played back 6 frames per sec (time-lapse factor is 18). The sequence start shows an infected PMN (with moving intracellular parasite) on top of a macrophage. The PMN is internalized, the parasite remains intact (moving) and the macrophage moves to the next infected PMN (containing 3 moving parasites).