Visualization of Antigen Presentation by Actin-Mediated Targeting of Glycolipid-Enriched Membrane Domains to the Immune Synapse of B Cell APCs
J Immunol Gordy et al.
172: 2030
Files in this Data Supplement:
Supplemental Movie 1 (QuickTime, 294 KB)
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Real time measurement of targeting of GEM domains to the immune synapse of APCs.
Fluorescence (top) and DIC (bottom) images of an antigen-pulsed CH27 cell expressing L10-GFP as it forms an immune synapse with a D10 T cell. Each frame was collected 1 min apart.
Supplemental Movie 2 (QuickTime, 1490 KB)
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Real time measurement of L10-GFP mobility in an MβCD-treated CH27 cell.
FRAP experiment of an MβCD-treated CH27 cell expressing L10-GFP. Each post-bleach frame was collected 7 s apart.
Supplemental Movie 3 (QuickTime, 1586 KB)
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Real time measurement of L10-GFP mobility in the immune synapse of a CH27 cell.
FRAP measurement of an antigen-pulsed CH27 cell expressing L10-GFP and conjugated to a D10 T cell. Each post-bleach frame was collected 7 s apart.
Supplemental Movie 4 (QuickTime, 3470 KB)
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Real time measurement of exchange of L10-GFP between the immune synapse and remaining compartments of the CH27 cell. FLIP experiment of a CH27 cell expressing L10-GFP and conjugated to a D10 T cell. A region of the immune synapse was repeatedly photobleached with 5 s pulses of laser illumination.