Files in this Data Supplement:

• Data Supplement Movie 1 (QuickTime Movie, 2,718 KB) - Web movie 1 and 2 are 20 min records of wild type DC interacting with naïve CD4+ T cells (movie 1: immature DCs pulsed with 10 nM H-Y peptide; movie 2: mature DC pulsed with 10 nM H-Y peptide).
• Data Supplement Movie 2 (QuickTime Movie, 2,966 KB) - See caption above.
• Data Supplement Movie 3 (QuickTime Movie, 1,383 KB) - Web movie 3 shows a 30 min records of I-A^b deficient mature DCs interacting with naïve T cells. Images were collected every 10 sec starting at 1 min after addition of T cells. Acceleration factor: 60X.