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LETTERS TO THE EDITOR |
Hybridon, Inc. Cambridge, MA 02139
Equils et al. (1) reported implication of simultaneous activation of Toll-like receptors (TLRs) on HIV replication. When spleen cells from HIV-1 transgenic mice were exposed to agonists of TLR2, TLR4, and TLR9, p24 production was induced. The agonist of TLR9 used in this study was bacterial CpG DNA.
In retrospect, these experimental observations could explain the consistent virus load increases found in plasma of HIV-1 infected individuals in Phase 1 dose-escalation trials conducted with GEM91 a few years ago.
GEM91, an antisense oligodeoxynucleotide phosphorothioate (5'-CTC TCG CAC CCA TCT CTC TCC TTC T-3') complementary to gag gene of HIV-1, showed potent inhibition of HIV-1 replication in cell cultures (2). In clinical trials, GEM91 was dosed by continuous i.v. infusion for 8 days in a blinded dose-escalation study. Plasma HIV-1 virus load measurements, expressed as mean log10 copies per milliliter by the bDNA method, were compared to pretreatment values for the six GEM91-treated subjects in each of the four highest dose groups. Results for the three placebo-treated subjects in each group were pooled. Plasma bDNA for placebo-treated subjects remained within 0.13 log10 of pretreatment baseline while mean plasma bDNA increased at 4 and 8 days in all four treated groups. By 6 days after the end of GEM91 infusions, the bDNA was again similar in GEM91 and placebo-treated subjects (Fig. 1).
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A second-generation antisense oligonucleotide (GEM92) was built on a truncated GEM91 sequence and the CpG motif was modified to neutralize its immunostimulatory activity. GEM92 has shown an improved safety profile in preclinical studies (3).
Based on the present evidence, it is likely that TRL9-activated immune responses to the CpG motif in GEM91 were responsible for the observed increases in viral load, and that CpG motif in oligonucleotide phosphorothioates is biologically active. Presently several antisense phosphorothioate oligodeoxynucleotides containing CpG motif are in human clinical trials for various diseases and indications.
References
Pediatric Infectious Diseases Steven Spielberg Pediatric Research Center Burns and Allen Research Institute Cedars-Sinai Medical Center University of California, Los Angeles School of Medicine Los Angeles, CA 90048
The Toll-like receptor (TLR) family mediates innate immune responses to pathogen-associated molecular patterns (PAMP) that are conserved molecular patterns produced by pathogens but not by their host. Bacterial DNA acts as a PAMP by virtue of a 20-fold greater frequency of unmethylated CG dinucleotides found in microbial DNA versus vertebrate DNA (R1 ). In addition, synthetic oligodeoxynucleotides (ODN) containing the proper CpG-DNA motif mimic the immunostimulatory effects of bacterial DNA (R2 ).
Genetic complementation studies using 293 cells and experiments using TLR9-deficient mice show that TLR9 is the receptor for bacterial CpG DNA (R3 R4 ). TLR9 response is CpG motif-dependent as the methylation of CpG DNA or inversion of the CpG ablates the responsiveness (R4 ). TLR9 also confers species-specific CpG response, and human PBMC respond best to GTCGTT motifs.
In the correspondence above, Drs. Agarwal and Martin show that GEM91, an antisense oligodeoxynucleotide phosphorothioate (5'-CTC TCG CAC CCA TCT CTC TCC TTC T-3') complementary to gag gene of HIV has similarities to CpG DNA, and possibly activates the cells through TLR9 to induce HIV replication. Aside from the presence of TCG, this sequence is quite pyrimidine rich, and would be effective through the TLR9 pathway at activating mouse and human immune cells (A. M. Krieg (University of Iowa, Iowa City, IA), personal communication).
Because of the ability of CpG-DNA to activate human dendritic cells and B cells (R5 R6 ) and induce Th1 response (R7 ) it is included in the design of newly developed vaccines. Our observation that CpG DNA induces HIV replication through TLR9 (R8 ), and Drs. Agarwal and Martin’s results suggest that CpG-DNA-like motifs included in the newly developed vaccines may have deleterious effects and in fact induce HIV replication. However the biological significance of these transient increases in HIV replication is yet to be determined.
References
This article has been cited by other articles:
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  A. Bafica, C. A. Scanga, M. Schito, D. Chaussabel, and A. Sher Influence of Coinfecting Pathogens on HIV Expression: Evidence for a Role of Toll-Like Receptors J. Immunol., June 15, 2004; 172(12): 7229 - 7234. [Abstract] [Full Text] [PDF]
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 C. Scheller, A. Ullrich, K. McPherson, B. Hefele, J. Knoferle, S. Lamla, A. R. M. Olbrich, H. Stocker, K. Arasteh, V. t. Meulen, et al. CpG Oligodeoxynucleotides Activate HIV Replication in Latently Infected Human T Cells J. Biol. Chem., May 21, 2004; 279(21): 21897 - 21902. [Abstract] [Full Text] [PDF]
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