Files in this Data Supplement:

• Supplemental Figure 1 (QuickTime Movie, 75KB) - Time-lapse imaging of a GEM-enriched membrane patch in a Jurkat T cell. Live cell imaging of a Jurkat cell expressing LckNT-GFP. Each frame was collected 20 sec apart.
• Supplemental Figure 2 (QuickTime Movie, 106KB) - Time-lapse imaging of a GEM-enriched membrane patch during T cell stimulation. Live cell imaging of a Jurkat cell expressing LckNT-GFP. The cell was stimulated with an OKT3-coated bead, and the experiment shows an LckNT-GFP-enriched patch migrating to a membrane cap that forms at the site of cell-bead contact. Each frame was collected 1 min apart.
• Supplemental Figure 3 (QuickTime Movie, 75KB) - Time-lapse imaging of diffusion of a GEM-enriched membrane patch in a T cell membrane cap. Live cell imaging of a Jurkat cell expressing LckNT-GFP. The experiment shows an LckNT-GFP patch migrating through the interface of the cell and an OKT3-coated bead. Each frame was collected 1 min apart.
• Supplemental Figure 4 (QuickTime Movie, 75KB) - Time-lapse imaging of diffusion of a GEM-enriched membrane patch in a stimulated D10 cell. Live cell imaging of a D10 T cell expressing L₁₀-GFP and stimulated with an antigen-pulsed CH27 cell. The frames were collected 10 s apart.