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* National Eye Institute and
National Cancer Institute, National Institutes of Health, Bethesda, MD 20892; and
Medical Genome Center, John Curtin School of Medical Research, Canberra, Australia
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Abstract |
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A-crystallin promoter develop tolerance due to thymic expression of minuscule amounts of HEL. To further address the mechanisms by which this tolerance develops, we mated these two lines of Tg mice with the 3A9 line of HEL-specific TCR Tg mice, to produce double-Tg mice. Both lines of double-Tg mice showed deletion of HEL-specific T cells, demonstrated by reduction in numbers of these cells in the thymus and periphery, as well as by reduced proliferative response to HEL in vitro. In addition, the actual deletional process in thymi of the double-Tg mice was visualized in situ by the TUNEL assay and measured by binding of Annexin V. Notably, the apoptosis localized mainly in the thymic medulla, in line with the finding that the populations showing deletion and increased Annexin V binding consisted mainly of single- and double-positive thymocytes. Interestingly, the thymic deletional effect of sHEL was superior to that of mHEL in contrast to the opposite differential tolerogenic effects of these HEL forms on B cells specific to this Ag. Analysis of bone marrow chimeras indicates that both forms of HEL are produced by irradiation-resistant thymic stromal cells and the data suggest that sHEL is more effective in deleting 3A9 T cells due mainly to its higher accessibility to cross-presentation by dendritic APC. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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The negative selection process is responsible for the elimination of self-reactive lymphocytes. Because the number of T cells specific to any self-Ag is normally too low to allow examination of the deletion process, the investigation of negative selection has been conducted on lymphocyte populations responsive to superantigens (1, 2) or on homogeneous populations of T cells with transgenic (Tg)3 TCR (2, 3, 4). Maturing thymocytes that express TCR with high affinity to self-Ags undergo apoptosis upon exposure to their specific target Ag (2, 3, 4, 5, 6), a process that takes place mainly in the thymic medulla and at the corticomedullary junction (4, 5, 7). Recent studies, in particular by Kyewski’s group, have identified three populations of thymic cells that present self-Ags in this process. These populations include medullary and, to a lesser extent, cortical epithelial cells and hematogenous dendritic cells (8, 9). Of particular interest are the medullary epithelial cells that were found to express numerous peripheral tissue-specific Ags, such as insulin or myelin proteolipid protein, and therefore play a crucial role in preventing autoimmune processes against these tissues (8, 9).
To investigate immunotolerance toward ocular-specific Ags, we have generated Tg mice that express a foreign Ag, hen egg lysozyme (HEL), under control of the lens A-crystallin promoter (10). These mice develop tolerance against HEL that is attributed to thymic expression of minuscule amounts of this Ag (10). Mating these HEL-Tg mice with Tg mice that express HEL-specific TCR on their T cells (designated "3A9" (11)) created double-Tg mice that allow further investigation of the process by which thymic deletion of HEL-specific T cells occurs. HEL is expressed transgenically in two forms, soluble (sHEL) and membrane-bound (mHEL) (12, 13) and studies by Goodnow et al. (12) have shown that the two forms differ remarkably in their tolerogenic effects on HEL-specific B cells. Whereas Tg expression of sHEL induced merely anergy in the HEL-specific B cell population (12), expression of mHEL produced tolerance by actually deleting these B cells (13). A more recent study by Goodnow’s group (14) and data collected in the present study show, however, that unlike with the B cell population, both forms of HEL produced deletion of T cells. Previous studies assessing T cell recognition of mHEL and sHEL have used Tg mice in which the two forms of HEL were expressed under different promoters (14). In our current study, however, we expressed both forms of HEL under control of the same promoter and, therefore, we could compare tolerance induction between the two forms of the Ag. We show here that sHEL is superior to mHEL in its capacity to delete HEL-specific T cells. The superiority of the soluble form of HEL in inducing immunotolerance was established by several parameters. Moreover, findings recorded here support the notion that Ag presentation by thymic stromal cells is not highly effective for tolerance induction and that cross-presentation is a major mechanism in thymic deletion.
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Materials and Methods |
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mHEL-Tg or sHEL-Tg mice in which the transgene is expressed under the A-crystallin promoter were generated as detailed elsewhere (10) and maintained in our facility on the FVB/N background. HEL-specific TCR Tg mice, on the B10.BR background, designated 3A9 (11), were a generous gift from Dr. M. Davis (Stanford University, Stanford, CA). Mice from each of the two HEL-Tg lines were mated with 3A9 mice to produce (FVB/N x B10.BR)F1 hybrids, expressing no transgene (wild type (WT), either one of the three transgenes (3A9, mHEL, or sHEL), or two transgenes ("mHEL double Tg" or "sHEL double Tg"). Only such F1 hybrid mice were used in all experiments of the present study.
Bone marrow chimeric mice were generated by irradiating recipient mice, as indicated, with 9.5 Gy, followed by i.v. injection with 5 x 106 donor bone marrow cells as indicated. Cells from the chimeric mice were examined 6 wk later.
The mice were housed in a pathogen-free facility and all manipulations were conducted in compliance with the National Institutes of Health Resolution on the Use of Animals in Research.
HEL measurement
Spleens from Ig-HEL-Tg mice, expressing IgM and IgD Abs/receptors for HEL (12), were minced through a cell strainer. The RBC were lysed using an ammonium chloride based solution. Then 0.5 x 106 cells were incubated for 30 min at 4°C with 50 µl of diluted blood serum or PBS extracts of eyes from 6-wk-old mice of the two Tg lines. The cells were washed three times and stained with anti-B220-FITC and HY-9-Tricolor conjugate (15), which specifically binds HEL at a different nonoverlapping epitope from the Ig-HEL transgene. Quantification was performed relative to a standard curve generated by measuring the level of soluble HEL binding. Specificity of HEL binding was assessed on splenocytes from non-Tg mice. Samples were acquired on a FACScan cytometer (BD Biosciences, Mountain View, CA) using CellQuest and analyzed on FlowJo software.
Quantitative RT-PCR analysis
First-strand synthesis was performed as described previously (16) using thymi of mHEL-Tg or sHEL-Tg mice, either untreated or 4 days following whole-body irradiation with 6.0 Gy. A negative control reaction without reverse transcriptase was performed for each RNA sample. RNA samples were normalized to 18S RNA using a TaqMan Ribosomal RNA Control Reagents kit (Applied Biosystems, Foster City, CA). Real-time PCR was performed on an ABI 7700 (Applied Biosystems) or ICycler iQ Real Time (Bio-Rad, Hercules, CA) Sequence Detection System with HEL-specific primers and probe: HEL forward primer, 5'-CGTGCTCAGCCCTGCTG-3'; HEL reverse primer, 5'-GCCGTTTCCATCGCTGAC-3'; and HEL hybridization probe, 6FAM-CGAGCGTGAACTGCGCGAAGAAGTAMRA. PCR primers and probes for GAPDH are described in the TaqMan Rodent GAPDH Control Reagents kit (Applied Biosystems). PCR parameters are as recommended for the TaqMan Universal PCR master mix kit (Applied Biosystems). Triplicate samples of 10-fold serial dilutions of cesium chloride-banded HEL plasmid cDNA were assayed and used to construct the standard curves.
Flow cytometry analysis
Directly labeled Abs specific for CD4, CD8, and Thy1.2 were purchased from BD PharMingen (San Diego, CA). The clonotype-specific Ab 1G12 that recognizes 3A9 T cells was a generous gift from Dr. E. Unanue (Washington University, St. Louis, MO). Single-cell suspensions were prepared and analyzed as previously described (17). Analysis of splenocytes and thymocytes from Tg mice was performed on a FACScan cytometer (BD Biosciences). Ten thousand events in a live gate were acquired although all ungated events were saved for later analysis.
Lymphocyte proliferation assay
Spleen or lymph node cells were collected and tested for their proliferative response against different HEL concentrations as described elsewhere (18), with the exception that the cultures were incubated for a total of 72 h. IL-2 (Chiron, Emeryville, CA) was added to certain cultures, as indicated, at 100 U/ml. Certain cultures were made of mixtures of naive splenocytes of WT mice and 3A9 mice as indicated.
Cellular proliferation assay via dye dilution
Spleen cells were washed extensively in PBS to remove residual protein, then 107 cells/ml were incubated in 1 µM CFSE (Molecular Probes, Eugene, OR) for 8 min at room temperature as previously described (19). Labeling reaction was quenched with an equal volume of FBS and the cells were centrifuged and washed three times in complete tissue culture medium consisting of 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin, 20 mM HEPES, 100 µM nonessential amino acids, 1 mM sodium pyruvate, and 25 µM 2-ME in RPMI 1640 (BioWhittaker, Walkersville, MD). CFSE-labeled cells were diluted in complete tissue culture medium and were cultured at 4 x 105 in 96-well U-bottom plates in the absence or presence of titrated HEL. Cultures were harvested after 72 h of incubation at 37°C in 5% CO2, stained with biotinylated 1G12 Ab and PE-labeled CD4 Ab for 30 min at 4°C, washed three times in FACS buffer (0.2% BSA, 0.01% sodium azide in HBSS without phenol red), and then incubated with avidin-CyChrome (BD PharMingen) for 10 min, followed by extensive washing in FACS buffer. CD4+ 1G12high and CD4+Ig12int cells were then gated and analyzed for dye dilution peaks. Cells were analyzed on a FACScan cytometer (BD Biosciences). Ten thousand events in a live gate were acquired although all ungated events were saved for later analysis.
Detection of apoptotic thymocytes ex vivo
Single-cell suspensions were resuspended in FACS buffer, then 1 x 106 cells were stained with the desired directly conjugated Ab for 30 min at 4°C, washed three times in FACS buffer, and then incubated with avidin-CyChrome (BD PharMingen) for 10 min, followed by extensive washing in FACS buffer. Cells were analyzed on a FACScan cytometer (BD Biosciences). Ten thousand events in a live gate were acquired although all ungated events were saved for later analysis. To detect apoptotic thymocytes, cells were then resuspended in annexin-binding buffer (BD PharMingen) immediately before analysis and labeled with Annexin V-PE per the manufacturer’s instructions (BD PharMingen) with the exception that Annexin V was diluted 1/5 rather than used undiluted.
TUNEL assay
The level of in situ apoptosis in mouse thymi was evaluated by the Trevigen Apoptotic Cell System (TACS) using the TACS 2 TdT Blue Label in situ Apoptosis Detection kit (Trevigen, Gaithersburg, MD). Briefly, Formalin-fixed, paraffin-embedded sections of mouse thymi were deparaffinized in xylene, then hydrated in graded ethanol concentrations. The protein was digested using cytopore (Trevigen) and then endogenase peroxide was blocked with 2% H2O2. The tissue was equilibrated with labeling buffer, then incubated with the labeling reaction mix for 45 min. The labeling reaction was terminated with stop buffer (Trevigen), then the sections were incubated with streptavidin-HRP conjugate. The sections were stained with Blue Label, followed by Red Counterstain C. The sections were dehydrated in graded ethanol, followed by xylene, then mounted with Permount (Fisher Scientific, Fairlawn, NJ). The number of apoptotic cells were counted in five different fields at x40 magnification, in a masked fashion, then averaged. Two to five mice were examined for each tested group.
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Results |
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Fig. 1 shows sections of eyes of sHEL- and mHEL-Tg mice. Whereas the sHEL-Tg mouse eyes exhibit normal morphology (Fig. 1A), eyes of the mHEL-Tg mice are dystrophic and much smaller in size, with disruption of the lens fibers and distortion of the lens capsule (Fig. 1C). No inflammation was detected, however, in eyes of either one of these single Tg mice.
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A-crystallin promoter, the main depot of HEL was found in the lens of these mice. Measurements of HEL in eyes and serum of the two lines of HEL Tg are summarized in Table I. The total HEL amount per eye was moderately higher in the mHEL-Tg mice than in the sHEL-Tg mice (232 vs 162 ng/eye), but the HEL concentration per milligram total protein was remarkably higher in the mHEL-Tg mice (545 vs 63 ng HEL/mg protein), since eyes of mHEL-Tg mice are dystrophic and contain much smaller amounts of total protein than eyes of sHEL-Tg mice (0.42 and 2.67 mg/eye, respectively). The two HEL-Tg lines also differed in their serum HEL content. Interestingly, in contrast to their lower HEL expression in the lens and intact lens capsule, sHEL-Tg mice had measurable amount of HEL in their serum, whereas no serum HEL was detected in the mHEL mice (Table I).
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A-crystallin promoter express the transgene in the thymus as well (10). Since the HEL protein levels are below the assay’s threshold, the presence of HEL in the thymus of the two HEL-Tg mouse lines was assessed according to the level of the corresponding HEL mRNA molecules using the real-time quantitative PCR assay. As seen in Fig. 2, the level of HEL mRNA transcripts in the mHEL-Tg mice was higher than that in the thymi of the sHEL-Tg mice by >50%. It should be noted that standard curves generated from the HEL cDNA dilution series showed excellent linearity, indicating a precise, quantitative relationship between cDNA copy number and fluorescence signal intensity within the dynamic range of the assay (data not shown).
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Double-Tg mice, created by mating the TCR-Tg 3A9 mice with either mHEL or sHEL single Tg mice, developed inflammatory ocular changes. These changes included in sHEL double-Tg mice moderate accumulation of inflammatory cells in the limbus, as well as perivascular cellular infiltration in the retina (Fig. 1B), seen in some mice of this group. Remarkably, more severe changes were seen in the mHEL double-Tg mice, with intense cellular infiltration in the limbus, retina. and vitreous as well as severe retinal edema (Fig. 1D). The histological changes in both groups of double-Tg mice peaked between 1 and 3 mo of age and gradually decreased thereafter.
Double-Tg mice resembled their corresponding parental HEL-Tg mice in their HEL levels (data not shown). As detailed below, the expression of HEL in the double-Tg mice affected profoundly their T cell populations.
Deletion of HEL-specific T cells in sHEL and mHEL double-Tg mice
To characterize the fate of HEL-specific T cells in the HEL double-Tg mice, we assessed their spleen content of 3A9 cells using the 1G12 clonotypic Ab. Spleen cells were gated for Thy1.2 expression, then assessed for expression of 1G12 and CD4. The number of 1G12-positive cells recovered in these mice was determined from total splenic cell recovery by the percentage of 1G12-positive cells. Both sHEL and mHEL double-Tg mice exhibited significant losses of CD4+ "clonotype high" (1G12high) cells (quadrant R3) compared with 3A9 controls (Fig. 3A). Interestingly, sHEL double-Tg mice also exhibited loss of CD4+ "clonotype intermediate" (1G12int) cells (quadrant R2) as well, whereas numbers of these cells were comparable between mHEL double-Tg and 3A9 mice. These findings demonstrate that elimination of clonotype-expressing cells is more complete in sHEL double-Tg mice than in mHEL double-Tg mice, with both high and low 1G12+ cells decreased in sHEL double-Tg mice, whereas deletion in the mHEL double-Tg mice is less complete, allowing cells with low expression of 3A9 TCR to escape deletion.
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Decreased lymphocyte responses to HEL in double-Tg mice
Naive lymphocytes of 3A9 mice proliferate vigorously upon stimulation with HEL in culture. Lymphocytes from the double-Tg mice also responded to HEL, but with lower intensity. As shown in Fig. 4, the reduced response was exhibited by lymphocytes from both lines of double-Tg mice, but the reduction was particularly clear in the sHEL double-Tg mice. Also of note is the observation that the reduced responsiveness became in general more apparent with age (Fig. 4). It is particularly interesting that the reduced response of the double-Tg mice was mostly pronounced at the lower range of HEL concentrations; the lowest HEL concentrations to stimulate mHEL or sHEL double-Tg lymphocytes were higher than those of 3A9 cells by at least 1 or 2 orders of magnitude, respectively.
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These two observations recorded in Fig. 5 thus support the notion that the lowered response of the double-Tg mice is because of the deletion of high-affinity clones from the repertoire.
The potential role of anergy in the reduced response to HEL of double-Tg mice was examined by adding exogenous IL-2 to HEL-stimulated cultures; anergic cells do respond in the presence of exogenous IL-2 (20). As seen in Fig. 6, a moderate enhancement of the response to HEL was repeatedly seen in IL-2-supplemented cultures of the sHEL double-Tg mice, but not in those of the mHEL double-Tg or the 3A9 controls. This finding suggests that the reduced response to HEL in sHEL double-Tg mice is partially attributable to development of anergy to HEL in these mice, but not in mHEL double-Tg mice.
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To test whether the loss of cells in thymi of double-Tg mice is because of deletion, we examined the thymic population of these mice for the binding of Annexin V, a marker for dying cells (21). Levels of Annexin V+ cells were elevated in both lines of double-Tg mice over those observed in 3A9 mice (Fig. 7A, "total" cells). Numbers of Annexin V+ cells in 3A9 thymi resembled those in WT mice or in single HEL-Tg mice (data not shown). Further analysis of apoptosis in thymocyte subsets revealed a dramatic increase in apoptosis in CD4 SP thymocytes from the sHEL double-Tg mice and a lower but substantial increase in apoptosis of these cells among the mHEL double-Tg mice. The percentage of Annexin V+ thymocytes also increased in DP thymocytes, with higher levels present in sHEL double-Tg mice as compared with mHEL double-Tg mice. Notably, no difference in apoptosis levels were observed in the double-negative population compared with 3A9 controls. These results demonstrate that, indeed, clonal deletion is ongoing in thymi of the double-Tg mice as early as the DP stage in differentiation and is more prevalent in the transgenics with sHEL than in mHEL.
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The TUNEL method that identifies apoptotic cells (22) provided us with an additional approach to evaluate the level of thymic apoptosis and, importantly, enabled us to localize the thymic areas in which the enhanced apoptosis takes place. Fig. 7C demonstrates typical intense TUNEL reaction in the thymus of sHEL double-Tg mice. The elevated apoptosis in thymi of double-Tg mice was quantified by counting TUNEL-positive cells in thymus sections of the three mouse lines collected at three different ages. The data, summarized in Fig. 7B, show that the apoptosis level in the 3A9 control mice was profoundly lower than in the double-Tg mice and that cell death in sHEL double-Tg thymi was higher than that in the mHEL double-Tg mice. Of particular interest is the observation that apoptotic cells in thymi of the double-Tg mice localized mainly in the medulla (Fig. 7, C and D), unlike the even distribution of the lower numbers of apoptotic cells throughout the thymus in 3A9 mice (data not shown). The selective localization of apoptotic cells in the medulla of double-Tg mice is further underscored by our finding that the enhanced apoptosis in 3A9 mice injected systemically with HEL localized mainly in the thymic cortex (Fig. 7E).
HEL is produced by thymic stromal cells
One possible explanation for the surprising ability of the sHEL transgenics to develop more extensive tolerance than mHEL transgenics could be that the APC that presents HEL is different when the HEL is soluble vs membrane bound. Thymic epithelial cells were shown to be the producers of Ags specific to peripheral organs (8, 9) and it has been proposed that Ags they release are captured by bone marrow-derived dendritic cells, the highly potent inducers of tolerance via clonal deletion (23, 24). To determine the cellular source of HEL in thymi of HEL-Tg mice, bone marrow chimeras were created in which bone marrow from the two lines of double-Tg mice and from 3A9 controls were transferred into groups of lethally irradiated WT recipients, while bone marrow from 3A9 donors was injected into irradiated sHEL-Tg or mHEL-Tg mice.
Analysis of splenic T cells, 6 wk after reconstitution (Fig. 8A), revealed a decrease in the recovery of CD4+ clonotypichigh cells (quadrant R3) when HEL transgenics served as the recipients (Fig. 8A). Moreover, deletion of these cells was notably more effective in 3A9
sHEL Tg (1.1 x 106 cells) than in 3A9mHEL Tg (20.8 x 106 cells). On the other hand, the recovery profiles of clonotypichigh cells when either one of the double-Tg mice was used as bone marrow donors (54.3 x 106 and 34.2 x 106 cells, respectively) resembled those in recipients of 3A9 donors (46.5 x 106). This pattern of differences among the various bone marrow chimeras was also observed when 1G12 levels were measured on spleen cells of these mice (Fig. 8B). No significant differences in cell numbers were observed in either the CD4+1G12low (quadrant R2) or CD4-1G12+ (quadrant R1) subpopulations of the different chimeras (Fig. 8A).
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The production of HEL by radiation-resistant stromal cells was further demonstrated by measuring HEL mRNA in thymi of mHEL and sHEL mice 4 days following irradiation with 6.0 Gy using real-time PCR. The numbers of copies per cell were 234 ± 44 and 125 ± 22 in mHEL and sHEL mouse thymi, respectively; namely, approximately four times higher than those measured in thymi of the corresponding intact mice, as recorded in Fig. 2. It is also noteworthy that in line with the observation with thymi of intact mice, the mRNA values are significantly higher in mHEL-Tg than in sHEL-Tg mice.
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Discussion |
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It is noteworthy that low levels of serum HEL were detected in sHEL-Tg mice but not in mHEL-Tg mice (Table I). Although it is possible that serum HEL contributed to the deletion of T cells in sHEL double-Tg mice, it is unlikely that the effect was substantial; extensive deletion of 1G12+ cells was found in the thymus of these mice (Fig. 3B), along with intense apoptosis in the medulla (Fig. 7C), where the serum HEL is not effective (Fig. 7E). Our assumption is also in line with the data of Klein et al. (25) showing that tolerance to a neo-self-Ag is determined mainly by thymic deletion, irrespective of differing serum levels of the Ag.
In addition to deletion of HEL-specific T cells, sHEL double-Tg mice also exhibited a moderate level of T cell anergy (Fig. 6). No lymphocyte anergy was detected in mHEL double-Tg mice (Fig. 6), suggesting that the form of the Ag determines the induction of anergy. This notion is also in line with a similar observation made by Akkaraju et al. (14). It remains to be determined as to whether anergy induction occurs thymically or peripherally in the sHEL double-Tg mice.
Actual deletion of HEL-specific T cells in the thymus was visualized and measured in this study by the TUNEL- and Annexin V-binding assays; it is assumed that the increased apoptosis seen in double-Tg mice is because of programmed death of maturing thymocytes expressing the 3A9 TCR upon their exposure to HEL. Indeed, a good correlation is seen in the present study between the level of elevated apoptosis in thymi of the two lines of double-Tg mice (Fig. 7, A and B) and the reduction in numbers of cells stained with the 3A9 clonotypic 1G12 Ab (Fig. 3), as well as the decrease in the lymphocyte responsiveness to HEL of these mouse lines (Fig. 4).
The TUNEL assay also provides information concerning the location at which clonal deletion of T cells takes place. In line with observations by Wack et al. (26) and Douek et al. (27), the elevated apoptosis in both lines of double-Tg mice localized mainly in the thymic medulla and the cortex/medulla junction. This observation is also in accord with reports by Kyewski, Hanahan, Pugliese, and their coworkers that medullary cells in thymi of mice (9, 28) or humans (29) express various tissue-specific or neo-self-Ags. It is of note that, unlike in double-Tg mice, the elevated apoptosis in single-Tg 3A9 mice following a bolus injection of HEL localized mainly in the thymic cortex (Fig. 7E), a finding in line with the observation by Wack et al. (26). As shown by Martin and Bevan (30), the massive apoptosis in thymic cortex of systemically injected 3A9 mice could be attributed to both Ag-specific deletion of thymocytes and the toxic effect of cytokines released by activated lymphocytes in the periphery. Taken together, these observations indicate that clonal-specific deletion is mediated by HEL synthesized in situ rather than by circulating Ag.
The selective location of the apoptotic process in the thymic medulla is also confirmed by our finding (Fig. 7A) that binding of Annexin V, an indicator for dying cells (21), was observed mainly in SP thymocytes, cells that are mostly located in the medulla. The observation that Annexin V binding was also seen in DP thymocytes (Fig. 7A) suggests that apoptosis also took place in the deep layers of the cortex.
Analysis of the proliferative response of spleen cells from the different Tg mouse lines further established the deletion of HEL-specific T cells in the double-Tg mice and the superior deletional process in the sHEL-Tg mice. Our data also confirm the notion (2, 3, 4, 5) that the negative selection mechanism eliminates mostly the thymocytes that express TCR with high affinity/avidity. The selective deletion was indicated by the skewed response toward higher HEL concentrations in cultures of double-Tg lymphocytes (Fig. 4) and was further verified by our findings using the CFSE dilution technique (Fig. 5B) and cultures of mixed cells from Tg and WT mice (Fig. 5A). Both assays demonstrated that lymphocytes from double-Tg mice failed to respond mostly against low HEL concentrations.
It is noteworthy that a correlation is seen in this study between the loss of lymphocyte response to low HEL concentrations in culture (Figs. 4 and 5) and the loss of 1G12high T cells in the double-Tg mice (Fig. 3A), apparently attributable to selective thymic deletion. Assuming that Tg TCR molecules are homogeneous in their secondary and even tertiary structures, we propose, therefore, that the negative selection of thymocytes with Tg TCR deletes selectively cells with high-density TCR in a process analogous to deletion of thymocytes with high affinity in WT animals. It is further assumed that the 1G12int thymocytes also express endogenous TCR.
Double-Tg mice were found in this study to develop ocular inflammation, characterized mainly by cellular accumulation of lymphoid cells, in and around blood vessels (Fig. 1, B and D), as well as retinal edema in the mHEL double-Tg mice (Fig. 1D). It is of note that local accumulation of lymphocytes and mild inflammation were observed by Akkaraju et al. (14) in double-Tg mice expressing HEL in the pancreas or thyroid. Our finding that the pathological changes were more severe in mHEL double-Tg mice (Fig. 1) could be explained by both the larger numbers of HEL-specific T cells in these mice than in the sHEL double-Tg mice and the phenotypic ocular changes only in mHEL-Tg mice. These changes include dystrophy and release of HEL from the lens (Fig. 1C and Ref.10) that facilitate the immunopathological process (31). Additional studies are in progress to define the features characterizing the inflammatory ocular changes and the cells accumulating in the affected mouse eyes.
Experiments with bone marrow chimeras have provided additional useful information concerning the deletion process. In accord with other reports, using various Ags and experimental approaches (23, 24, 25, 32), we found that both forms of HEL are expressed by radiation-resistant stromal thymus cells, but not by bone marrow-derived cells (at least not at levels capable of inducing deletion). The involvement of thymic dendritic cells and macrophages in clonal deletion has been controversial (33, 34, 35). Several publications have shown that thymic dendritic cells or macrophages do express peripheral Ags (29, 33, 34), but their capacity to induce deletion apparently depends on the cellular location of the Ag. Klein et al. (36) have shown that thymic hemopoietic cells are highly capable of deleting specific Th lymphocytes, but only when the Ag was introduced to the thymus via the blood stream (36). Moreover, data of Oukka et al. (37) indicate that bone marrow-derived thymic cells were incapable of causing deletion even when expressing transgenically the target Ag in their nuclei. This observation thus suggests that bone marrow-derived cells are incapable of presenting intracellular Ag via the MHC class II endogenous pathway.
Since dendritic cells are the most potent APC, it has been suggested and experimentally shown that these cells participate in the thymic deletion process by the mechanism of cross-presentation, i.e., dendritic cells present efficiently Ags produced by stromal epithelium (see Ref.23). Cross-presentation does not take place in all systems however, as demonstrated in the study of Klein et al. (36). Data reported in the present study may therefore shed new light on this issue by showing the importance of the biophysical properties of the Ag. We thus propose that the superior tolerogenicity of sHEL derives from its being efficiently cross-presented by thymic dendritic cells following release from the medullary epithelial cells. mHEL, on the other hand, is assumed to be presented to the T lymphocytes mainly by the epithelial cells that are less efficient APC than dendritic cells. The capacity of epithelial cells to autonomously present Ag and induce tolerance was elegantly demonstrated by Oukka et al. (37).
In conclusion, the present study compared for the first time the capacity of soluble and membrane-bound forms of an Ag, HEL, to induce thymic deletion and T cell tolerance. sHEL was found to be superior to mHEL by all parameters employed, testing the actual deletion of HEL-specific T cells as well as its outcome, i.e., the reduction in numbers of HEL-specific T cells and in responsiveness to the Ag. This observation is remarkable in view of the finding that the membrane-bound form of HEL is more effective than the soluble form in inducing tolerance and deletion of B cells. Both forms of HEL are produced by thymic stromal cells and the superiority of sHEL in deleting T cells supports the model in which negative selection achieves high efficiency when the self-Ag is transferred from thymic epithelial cells to the highly potent APC, the bone marrow-derived dendritic cells.
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Acknowledgments |
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Footnotes |
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2 Address correspondence and reprint requests to Dr. Igal Gery, National Eye Institute, National Institutes of Health, Building 10, Room 10N112, Bethesda, MD 20892-1857. E-mail address: igery{at}helix.nih.gov
3 Abbreviations used in this paper: Tg, transgenic; HEL, hen egg lysozyme; mHEL, membrane-bound HEL; sHEL. soluble HEL; WT, wild type; SP, single positive; DP, double positive.
Received for publication July 15, 2002. Accepted for publication February 3, 2003.
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References |
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peptide-I-Ab complex by isolated thymic stromal cells. Int. Immunol. 6:1949.This article has been cited by other articles:
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C. A. Garcia, K. R. Prabakar, J. Diez, Z. A. Cao, G. Allende, M. Zeller, R. Dogra, A. Mendez, E. Rosenkranz, U. Dahl, et al. Dendritic Cells in Human Thymus and Periphery Display a Proinsulin Epitope in a Transcription-Dependent, Capture-Independent Fashion J. Immunol., August 15, 2005; 175(4): 2111 - 2122. [Abstract] [Full Text] [PDF]
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 H. Takase, C.-R. Yu, R. M. Mahdi, D. C. Douek, G. B. DiRusso, F. M. Midgley, R. Dogra, G. Allende, E. Rosenkranz, A. Pugliese, et al. Thymic expression of peripheral tissue antigens in humans: a remarkable variability among individuals Int. Immunol., August 1, 2005; 17(8): 1131 - 1140. [Abstract] [Full Text] [PDF]
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 K. Matsumoto, G. R. Leggatt, J. Zhong, X. Liu, R. L. de Kluyver, T. Peters, G. J. P. Fernando, A. Liem, P. F. Lambert, and I. H. Frazer Impaired Antigen Presentation and Effectiveness of Combined Active/Passive Immunotherapy for Epithelial Tumors J Natl Cancer Inst, November 3, 2004; 96(21): 1611 - 1619. [Abstract] [Full Text] [PDF]
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A. Liston, D. H.D. Gray, S. Lesage, A. L. Fletcher, J. Wilson, K. E. Webster, H. S. Scott, R. L. Boyd, L. Peltonen, and C. C. Goodnow Gene Dosage-limiting Role of Aire in Thymic Expression, Clonal Deletion, and Organ-specific Autoimmunity J. Exp. Med., October 18, 2004; 200(8): 1015 - 1026. [Abstract] [Full Text] [PDF]
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A. M. Gallegos and M. J. Bevan Central Tolerance to Tissue-specific Antigens Mediated by Direct and Indirect Antigen Presentation J. Exp. Med., October 18, 2004; 200(8): 1039 - 1049. [Abstract] [Full Text] [PDF]
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P. Sinha, H. H. Chi, H. R. Kim, B. E. Clausen, B. Pederson, E. E. Sercarz, I. Forster, and K. D. Moudgil Mouse Lysozyme-M Knockout Mice Reveal How the Self-Determinant Hierarchy Shapes the T Cell Repertoire against This Circulating Self Antigen in Wild-Type Mice J. Immunol., August 1, 2004; 173(3): 1763 - 1771. [Abstract] [Full Text] [PDF]
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 D.-I. Ham, S. J. Kim, J. Chen, B. P. Vistica, R. N. Fariss, R. S. Lee, E. F. Wawrousek, H. Takase, C.-R. Yu, C. E. Egwuagu, et al. Central Immunotolerance in Transgenic Mice Expressing a Foreign Antigen under Control of the Rhodopsin Promoter Invest. Ophthalmol. Vis. Sci., March 1, 2004; 45(3): 857 - 862. [Abstract] [Full Text] [PDF]
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 P. N. Pichurin, O. Pichurina, R. C. Marians, C.-R. Chen, Terry. F. Davies, B. Rapoport, and S. M. McLachlan Thyrotropin Receptor Knockout Mice: Studies on Immunological Tolerance to a Major Thyroid Autoantigen Endocrinology, March 1, 2004; 145(3): 1294 - 1301. [Abstract] [Full Text] [PDF]
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cpm ± SEM of responses of three mice of each mouse line, at each tested age, in three different experiments.
