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Tumor-Derived TGF- Reduces the Efficacy of Dendritic Cell/Tumor Fusion Vaccine¹

Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor, Michigan 48109

	Abstract

Top Abstract Introduction Materials and Methods Results Discussion References

 
Dendritic cell (DC)-based antitumor vaccine is a novel cancer immunotherapy that is promising for reducing cancer-related mortality. However, results from early clinical trials were suboptimal. A possible explanation is that many tumors secrete immunosuppressive factors such as TGF-, which may hamper host immune response to DC vaccine. In this study, we demonstrated that TGF- produced by tumors significantly reduced the potency of DC/tumor fusion vaccines. TGF--secreting (CT26-TGF-) stable mouse colon cancer cell lines were generated using a retroviral vector expressing TGF-. A non-TGF--secreting (CT26-neo) cell line was generated using an empty retroviral vector. The efficacies of DC/tumor fusion vaccines were assessed in vitro and in vivo. DC/CT26-TGF- fusion cells failed to induce a strong T cell proliferative response in vitro, mainly due to the effect of TGF- on T cell responsiveness rather than DC stimulatory capability. Animals vaccinated with DC/CT26-TGF- fusion vaccine had lower tumor-specific CTL activity and had significantly lower survival after tumor challenge as compared with animals immunized with DC/CT26-neo hybrids (45 vs 77%, p < 0.05). Ex vivo exposure of DCs to TGF- did not appear to lessen the efficacy of DC vaccine. These data suggest that tumor-derived TGF- reduces the efficacy of DC/tumor fusion vaccine via an in vivo mechanism. Neutralization of TGF- produced by the fusion cells may enhance the effectiveness of DC-based immunotherapy.

	Introduction

Top Abstract Introduction Materials and Methods Results Discussion References

 
The use of tumors as immunogens to induce antitumor immunity has become a major focus of cancer research. The dendritic cell (DC)³-based antitumor vaccine has emerged as a promising cancer immunotherapy with proven clinical efficacy (1, 2, 3, 4, 5). DCs are specialized APCs that can induce both the generation and proliferation of specific CTLs and Th cells through Ag presentation via MHC class I and class II molecules, respectively (6, 7, 8, 9, 10).

Considering the expected emergence of Ag-loss variants and the fact that most tumor Ags are not well-defined, DC-based vaccines often are generated using whole tumor-derived material (e.g., live tumors or tumor lysates). The fusion of tumor cells and DCs has been shown to induce strong immunologic responses (1, 11, 12), especially in animal studies; preliminary results from early clinical trials, however, have been disappointing. In one study, 60% of patients treated with DC/renal cell tumor fusion cells did not respond (1).

One disadvantage of using whole tumor substrate is that it may contain immunosuppressive factors that reduce vaccine efficacy. It has been documented that many tumor cell lines constitutively produce TGF-, IL-10, or PGE-E₂ (13, 14, 15, 16, 17). In vitro studies have shown that these molecules inhibit NK cell- or lymphokine-activated killer cell-mediated cytotoxicity, or they inhibit IL-2 production (13, 18). Furthermore, TGF- has been shown to inhibit CTL generation (19). The failure of the immune system to recognize and destroy tumors may in part result from the suppression of immune function by these factors (20). Whether immunosuppressive factors secreted by tumors also paralyze the induction of antitumor immunity by DC vaccines, leading to treatment failure, is not known.

Previous studies of the effect of TGF- on DCs have been inconclusive (21). Although one study showed that in vitro DC generation required TGF- (22), other studies have shown that TGF- can block DC maturation (23). Yet another study suggested that TGF- reversibly regulates chemotaxis of DCs by regulating chemokine receptor expression (24). DCs infected with an adenoviral vector expressing TGF- and IL-10 were found to induce partial immune tolerance; however, TGF- alone did not (25). A recent study showed that TGF- did not significantly impair the T cell stimulatory capacity of DCs in vitro (26).

To demonstrate the effect of TGF- on DC antitumor vaccines, we generated a stable TGF--secreting tumor cell line using a retroviral vector encoding the constitutively active form of TGF-. A non-TGF--secreting parental tumor cell line generated from the backbone vector was used as the control. Fusion vaccines generated separately using these two cell lines were evaluated in vitro and in vivo. We demonstrated that tumor-derived TGF- reduced the ability of the fusion vaccine to stimulate T cell proliferation in vitro, inhibited the induction of the CTL response in vivo and weakened the protective ability of the DC vaccine against tumor challenge. To our knowledge, this is the first study to elucidate the inhibitory effect of tumor-derived immunosuppressive factors on DC/tumor fusion vaccines. These results suggest that blocking tumor-derived immunosuppressive factors may improve the clinical efficacy of DC vaccines.

	Materials and Methods

Top Abstract Introduction Materials and Methods Results Discussion References

 
Mice

Female BALB/c mice aged 6–8 wk were purchased from The Jackson Laboratory (Bar Harbor, ME) and housed at the Animal Maintenance Facility at the University of Michigan Health System (Ann Arbor, MI). Experiments were conducted at age 10–14 wk.

Media and cytokines

Complete medium (CM) consisted of RPMI 1640 with 10% heat-inactivated FCS, 2 mM glutamine, 100 U/ml penicillin, and 100 µg/ml streptomycin. CTL medium consisted of CM with 2-ME (Life Technologies, Grand Island, NY). Recombinant cytokines (R&D Systems, Minneapolis, MN) were diluted in CM: mouse GM-CSF (10 ng/ml), mouse IL-4 (10 ng/ml), human IL-2 (20 U/ml).

Tumor cell lines

CT26, a mouse colon adenocarcinoma cell line (27), and Renca, a mouse renal epithelial adenocarcinoma cell line (27), both grown in BALB/c mice, were propagated in CM.

Generation of a TGF--expressing CT26 cell line

A CT26 cell line stably transfected to express a constitutively active form of TGF-1 (CT26-TGF-) was generated using a retrovirus that expressed TGF- and a neomycin-resistant gene, kindly provided by Dr. G. Nabel (Vaccine Research Center, National Institutes of Health, Bethesda, MD) (28). A control CT26 cell line (CT26-neo) was generated similarly using the retrovirus backbone containing the neomycin-resistant gene alone.

Generation of bone marrow-derived DCs

Erythrocyte-depleted murine bone marrow cells were cultured in CM with 10 ng/ml GM-CSF and 10 ng/ml IL-4 at 1 x 10⁶ cells/ml, as described (29). On day 6, nonadherent DCs were harvested by forceful pipetting and enriched by metrizamide centrifugation gradients (14.5% by weight) (Sigma-Aldrich, St. Louis, MO). The low density interface containing the DCs was collected by gentle pipette aspiration. The recovered DCs were washed twice with RPMI 1640 and cultured in CM with GM-CSF and IL-4.

DC/tumor fusion cell preparation

Bone marrow-derived DCs were fused with tumor cells at a DC-tumor ratio of 2:1 using 50% polyethylene glycol (PEG; m.w. 1450)/DMSO solution (Sigma-Aldrich), as described (28). Briefly, the DC/tumor cell suspension was washed twice with RPMI 1640 prewarmed to 37°C. PEG (50%, 1 ml) was added over 1 min and the suspension was stirred gently for 1–2 min. Prewarmed RPMI 1640 (1 ml) was then added over 1 min and the suspension was stirred. An additional 3 ml of RPMI 1640 was added over 3 min, after which 10 ml of RPMI 1640 was added slowly. After a 5-min incubation at 37°C, the resultant cell mixture was pelleted and grown overnight in CM with GM-CSF and IL-4.

Determination of fusion efficiency

DCs were incubated with Mouse Fc Block (BD PharMingen, San Diego, CA) 1 µg/100 ml for 20 min at 4°C followed by labeling with R-PE-conjugated anti-mouse CD11c (BD PharMingen) or PE-conjugated IgG isotype control (BD PharMingen). Tumor cells (CT26-neo or CT26-TGF-) were labeled with a PKH67-Green Fluorescent Cell Linker kit (Sigma-Aldrich) (30). DCs and tumor cells were then fused with 50% PEG as described above. The fusion efficiency was determined by counting the percent of double-stained cells using Coulter Epics XL Cytometer (Hialeah, FL). The number of intact fused cells following fusion was assessed by the trypan-blue exclusion test.

TGF- production by fusion cells

DC/tumor fusion cells were sorted using Coulter Elite ESP Sorter (Coulter) and cultured at 1.5 x 10⁵ cells/ml for 48 h in CM supplemented with IL-4 and GM-CSF. Bioactive TGF- secretion was determined by ELISA without acidification of the samples.

Tumor lysate-pulsed DC preparation

DCs were pulsed with freeze-thawed tumor lysates at a DC-tumor ratio of 1:3, as described (31). CT26-TGF- pulsed DCs and CT26-neo pulsed DCs were harvested and washed two times after an 18-h incubation.

Induction of proliferative T cell responses in vitro

Irradiated (16,000 rad) DC/tumor fusion cells were cocultured with naive syngeneic BALB/c splenocytes (1 x 10⁴ cells) for 48 h. Alternatively, before fusion with DCs, tumors were pretreated with mitomycin C (Sigma-Aldrich) instead of radiation. Tritiated deoxythymidine (1 µCi/well; Amersham Biosciences, Piscataway, NJ) was added to each microtiter well and the plates were incubated for 24 h. At completion, the plates were harvested and the radioactivity was measured using a scintillation counter. Responses were reported as mean cpm ± SEM from duplicate samples.

Animal studies

BALB/c mice were vaccinated twice (i.p.) on days 0 and 14 with DC/CT26-TGF- fusion cells, DC/CT26-neo fusion cells, CT26-TGF--pulsed DCs, or CT26-neo-pulsed DCs (5 x 10⁵ cells per injection). To test the efficacy of the vaccines, the animals were challenged with a lethal dose of CT26 (2 x 10⁶ cells) on day 21. Animals were checked for tumors twice weekly, and tumor growth and survival times were recorded. Tumors were measured with a pair of Vernier calipers twice weekly and size was calculated according to the following equation: volume = b² x a/2 where a is the longer diameter of the tumor and b the shorter diameter.

CTL assays

T cells enriched by nylon wool column separation were tested for cytolytic activity in a standard 6-h ⁵¹Cr-release assay. CTLs (15 x 10⁶ cells) were stimulated with irradiated tumor (12,000 rad) at a T cell-tumor ratio of 20:1 in CTL medium for 5 days. IL-2 (20 U/ml) was added on day 2. Target cells (1 x 10⁶ cells) were incubated at 37°C with ⁵¹Cr (50 µCi) (Amersham Biosciences) in 100 µl of CTL medium for 1 to 2 h, washed twice with RPMI 1640, and resuspended in CTL medium at 1 x 10⁵ cells/ml. Target cells labeled with ⁵¹Cr (1 x 10⁴ cells/100 µl) were placed in each well of 96-well plates, and 100 µl of effector T cells for each dilution was added. Plates were incubated for 6 h at 37°C. The supernatant from each well was harvested and the amount of ⁵¹Cr radioactivity released was measured in a gamma counter. The percentage of specific lysis was calculated.

Serum TGF- concentration in animals that received a single immunization

Animals were anesthetized and whole blood was collected from tail veins 6 and 14 days after a single immunization with 5 x 10⁵ DC/CT26-TGF- fusion cells or DC/CT26-neo fusion cells. Time points earlier than 6 days (day 1 and 3) were obtained in a separate experiment. Serum samples were acidified with 1 M HCl (pH 2.0) as described (32), incubated at 4°C for 1 h, and neutralized with 1 M NaOH (pH 7.0) shortly before use. TGF- concentration in each sample was determined using a standard ELISA (R&D Systems).

Statistical analysis

Unpaired Student’s t test analyses were performed using GraphPad Prism version 3.00 for Windows (GraphPad Software, San Diego CA).

	Results

Top Abstract Introduction Materials and Methods Results Discussion References

 
Generation of TGF--secreting tumor cell line

To demonstrate the effect of TGF- on the DC-tumor fusion vaccine, CT26 tumor cells were genetically modified to overexpress TGF-. The successful generation of CT26-TGF- was confirmed by detecting the TGF- level in the tumor cell supernatant (Fig. 1). CT26-TGF- cells secreted 10 times more TGF- than CT26-neo cells (11 ng/ml vs 0.4 ng/ml). The enhanced TGF- expression of CT26-TGF- is comparable to some murine and human cancer cell lines known to spontaneously secrete high TGF- levels (20, 33).

View larger version (9K): [in this window] [in a new window]   FIGURE 1. TGF- expression of CT26-TGF- and CT26-neo cells. Tumor cells, CT26-TGF- or CT26-neo, were seeded at 5 x 105 cells/ml in a 6-well plate and cultured for 48 h in CM. Supernatants were collected and acidified as described in Materials and Methods. TGF- concentration in each supernatant was determined by ELISA. Results are representative of three independent experiments.

DC vaccine derived from TGF--secreting tumor continue to secrete TGF-

To generated DC/tumor fusion vaccines, CT26-neo or CT26-TGF- were fused with DC cells as described in Materials and Methods. To determine the fusion efficiency, DCs were stained with PE-conjugated anti-mouse CD11c and tumor cells were labeled with a PKH67-Green Fluorescent Cell Linker kit as previously described (30). The fusion efficiency was determined by the percent of double-stained cells using FACS analysis (Fig. 2). The mean fusion efficiency was 21.3% (range 17–25.3%). This is consistent with the reported fusion efficiencies of 12.5–30% (34, 35, 36). We did not observe significant variation in the fusion efficiency between DC/CT26-neo and DC/CT26-TGF-. Approximately 50% of the fused cells were viable per trypan blue exclusion test (data not shown). To confirm that DC/CT26-TGF- fusion cells continued to secrete bioactive TGF-, flow sorted DC/CT26-TGF- fusion cells were cultured for 48 h and the supernatants were analyzed by ELISA. Because CT26-TGF- tumor cells constitutively secrete the active form of TGF-, the supernatants were not acidified; therefore, only tumor-derived TGF- was detected and not inactive TGF- produced by DCs. The supernatant from a cell culture containing only DCs served as the control. The concentrations of bioactive TGF- were 356 ± 5.35 pg/ml for sorted DC/CT26-TGF- fusion cells and were undetectable for DC alone (Fig. 2D). These results clearly demonstrated that DC/CT26-TGF- fusion cells continue to secrete TGF-.

View larger version (37K): [in this window] [in a new window]   FIGURE 2. Determination of fusion efficiency and TGF- secretion by DC/tumor fusion cells. A, FACS analysis showing CT26-TGF- tumor cells labeled with PKH67-green fluorescent dye before PEG treatment. B, DCs labeled with PE-conjugated anti-CD11c before fusion. C, PEG effectively fused DC and CT26-TGF- fusion cells. The fusion efficiency was determined by the percent of double-stained cells (25.7%). D, DC/CT26-TGF- fusion cells secrete bioactive TGF-. Fusion cells were sorted by a FACS sorter and 1.5 x 105 fusion cells/ml were cultured for 48 h. ELISA was performed on nonacidified supernatants to detect tumor-derived TGF-. The supernatant from DC culture (1.5 x 105 cells/ml) was included as the control.

DC vaccine derived from TGF--secreting tumor is less effective in inducing T cell proliferation in vitro

The inhibitory effect of tumor-derived TGF- on the DC vaccine was first demonstrated in vitro. We investigated the capability of DC/CT26-TGF- fusion cells and DC/CT26-neo fusion cells to prime naive syngeneic T cells by measuring the T cell proliferative response. Stimulation of naive syngeneic BALB/c T cells with DC/CT26-TGF- fusion cells failed to induce a significant proliferative response (Fig. 3). The failure of T cells to respond to DC/TGF- fusion cells in vitro suggests that tumor-derived TGF- may either reduce the ability of DCs to present Ag to T cells or directly suppress T cell proliferation without affecting DC.

View larger version (20K): [in this window] [in a new window]   FIGURE 3. DC/CT26-TGF- fusion cells failed to induce a strong T cell proliferation response in vitro. Naive T cells (2 x 105 cells/well) were cocultured with different numbers of irradiated DC/tumor fusion cells (DC/CT26-neo or DC/CT26-TGF-) and incubated for 48 h. T cell proliferation was determined by [3H]thymidine assay and expressed as proliferation index, defined as the ratio of the measured radioactivity in wells with T cells stimulated with DC/tumor fusion cells over that of T cell alone. No significant proliferative activity was detected in T cells alone or fusion cells alone (data not shown). Results are representative of two independent experiments.

Exposure of DCs to TGF- ex vivo did not affect DC T cell stimulatory capacity

To determine whether tumor-secreted TGF- affects the functional capacity of DCs, we cultured DC/CT26-neo fusion cells overnight with supernatants from DC/CT26-TGF- fusion cells and assessed the ability of the fusion cells to stimulate T cells. Overnight ex vivo exposure of DC/CT26-neo fusion cells to TGF- did not reduce the ability of fusion cells to stimulate T cell proliferation (Fig. 4). This finding is consistent with the report that exposure to TGF- does not inhibit the ability of DCs to present Ag in vitro (26).

View larger version (13K): [in this window] [in a new window]   FIGURE 4. Stimulatory effect of DC/CT26-neo fusion cells was unaffected by overnight culture in supernatant of DC/CT26-TGF-. DC/CT26-neo fusion cells were cultured in an overnight supernatant of DC/CT26-TGF- fusion cell culture. T cell proliferation assays were performed using [3H]thymidine and proliferative activity was expressed as cpm. Left panel, Three dilutions of DC/CT26-neo fusion cells (102, 103, and 104) were cocultured with naive T cells (104 cells). Right panel, Similarly, DC/CT26-neo fusion cells incubated overnight with supernatant containing tumor-derived TGF- were cocultured with naive T cells. Results are representative of two independent experiments.

DC vaccine derived from TGF--secreting tumor is less effective in inducing CTL activity in vivo

To determine the in vivo effect of tumor-derived TGF- on DC vaccine, animals were immunized with a single dose of DC/CT26-neo fusion cells or DC/CT26-TGF- fusion cells. The ability of the DC/tumor fusion vaccine to induce tumor-specific CTL activities was assessed by CTL assay. All three mice vaccinated with DC/CT26-neo fusion cells developed a stronger CTL response compared with the response in three of three mice vaccinated with DC/CT26-TGF- fusion cells (Fig. 5).

View larger version (20K): [in this window] [in a new window]   FIGURE 5. Induction of CTL response in vivo was reduced with DC/CT26-TGF- fusion vaccine. BALB/c mice (n = 3) were immunized once on day 0 with 5 x 105 DC/CT26-neo or DC/CT26-TGF- fusion cells. T cells obtained from the spleen on day 14 were stimulated with irradiated CT26 for 5 days. 51Cr-labeled CT26 cells were cocultured with stimulated CTL for 6 h at the indicated E:T ratios. CTL activity was expressed as the percent of cells killed. Each line on the graph represents an individual mouse.

DCs fused with TGF--secreting tumor cells are less effective in protecting mice from tumor challenge

The protective immunity induced by DC/tumor fusion cells was assessed in vivo by challenging mice with a lethal dose of CT26 after immunization. BALB/c mice were vaccinated with DC/CT26-TGF- fusion cells or DC/CT26-neo fusion cells (5 x 10⁵ cells) on days 0 and 14. An inoculation of CT26 (2 x 10⁶ cells, s.c.) was administered on day 21. Higher tumor incidence and lower survival rates occurred in mice immunized with DC/CT26-TGF- fusion cells, compared with those immunized with DC/CT26-neo fusion cells, p < 0.05 (Figs. 6 and 7, and Table I). All surviving animals remained tumor-free beyond 1 year.

View larger version (17K): [in this window] [in a new window]   FIGURE 6. Higher tumor incidence in mice immunized with DC/CT26-TGF- fusion vaccine. Mice (n = 22) were immunized twice with DC/CT26-TGF- or DC/CT26-neo fusion cells on days 0 and 14, and inoculated with CT26 (2 x 106 cells) on day 21. Tumor development was monitored and recorded as positive growth if tumor size was >5 mm in its largest diameter. Tumor incidence was significantly higher in the DC/CT26-TGF- group as compared with the DC/CT26-neo group (*, p < 0.05).

View larger version (17K): [in this window] [in a new window]   FIGURE 7. Lower survival rates in mice immunized with DC/CT26-TGF- fusion vaccine. BALB/c mice received two immunizations of either DC/CT26-neo fusion cells (n = 22) or DC/CT26-TGF- fusion cells (n = 22), as described in Fig. 6, or mice (n = 10) were not vaccinated. Animals were sacrificed once tumor size reached 15 mm in its largest diameter. Survival at 100 days after tumor challenge was significantly lower in the DC/CT26-TGF- group compared with the DC/CT26-neo group (*, p < 0.05).

Decreased efficacy of DC/CT26-TGF- fusion cell vaccine is not due to higher serum TGF- level

To better understand how tumor-derived TGF- reduces the ability of DC/CT26-TGF- fusion cells to protect against tumor challenge, we compared the serum TGF- level of mice immunized with DC/CT26-TGF- fusion cells to that of mice immunized with DC/CT26-neo fusion cells. A single vaccination was administered on day 0 and serum TGF- levels were compared on days 1, 3, 6, and 14. No significant difference in the total serum TGF- level was detected between the two groups for all four time points (Fig. 8 and data not shown). Because CT26-TGF- tumor cells constitutively secrete the active form of TGF-, further tests of nonacidified serum samples for the active form of TGF- were performed. We found no detectable levels of tumor-derived TGF- (data not shown). These results suggest that TGF- secreted from DC/CT26-TGF- fusion cells probably exerts its effect locally rather than systemically.

View larger version (20K): [in this window] [in a new window]   FIGURE 8. Serum TGF- levels in mice immunized with DC/tumor fusion vaccine. BALB/c mice (n = 3 in each group) were immunized once with DC/CT26-neo fusion cells or DC/CT26-TGF- fusion cells as described in Fig. 5. Sera collected on days 1 and 3 were analyzed by ELISA to determine TGF- levels. No significant difference was found between the two groups.

Exposure of DCs to TGF- ex vivo did not affect DC vaccine efficacy

The ability to generate tumor lysate-pulsed DC vaccines offers an opportunity to study the consequence of ex vivo TGF- exposure on the efficacy of DC vaccine. DCs were pulsed with the tumor lysate of either CT26-TGF-neo or CT26-TGF- overnight. The cells from each group were then washed to remove tumor-derived TGF- and used to immunize mice (two doses separated by 14 days). Seven days after the second vaccination, animals were challenged with a lethal dose of CT26. The posttumor challenge survival rates between the two groups were similar, suggesting that overnight exposure of DCs to TGF- ex vivo did not affect the ability of DCs to induce tumor-rejecting CTL activities (Table I). Interestingly, our results, although not statistically significant, also revealed that the DC/CT26-neo fusion vaccine tends to be more effective than the CT26-neo lysate-pulsed DC vaccine in preventing tumor growth (77.3 vs 41.2; p > 0.05).

	Discussion

Top Abstract Introduction Materials and Methods Results Discussion References

 
The potential of DC-based vaccines to eradicate unresectable tumors has stimulated intense investigation. Most studies have focused on improving vaccine efficacy using adjunct treatment or the expression of cytokines and costimulatory molecules (37, 38, 39, 40, 41, 42, 43, 44, 45, 46). Little is known about the impact of tumor-derived immunosuppressive factors on the efficacy of the DC vaccine. Considering that many tumors express immunosuppressive factors and that DC/tumor fusion vaccines are being used in clinical settings, we addressed the question of whether tumor-derived immunosuppressive factors could limit the efficacy of the DC vaccine. We observed that the tumor-specific CTL response induced by DCs fused with TGF--expressing tumor was decreased both in vitro and in vivo. Furthermore, protection against tumor challenge was significantly compromised suggesting that tumor-derived immunosuppressive factors can significantly reduce the efficacy of the DC/tumor fusion vaccine.

The findings that TGF- in tumor lysate did not significantly affect DC vaccine efficacy suggest that overnight exposure of DCs to TGF- in vitro does not reduce DC Ag-presenting capability. This was further supported by the finding that culturing DC/CT26-neo fusion cells with supernatant from the DC/CT26-TGF- fusion cell culture did not affect the ability of DC/CT26-neo fusion cells to simulate T cell proliferation in vitro (Fig. 4). Thus, the most likely mechanism for the reduced efficacy of the DC/CT26-TGF- fusion vaccine is the in vivo effect of TGF- on the responsiveness of T cells to DC priming. Failure to detect increased TGF- levels in the sera of mice immunized with the DC/CT26-TGF- fusion vaccine further suggests that the amount of TGF- in the microenvironment of lymphoid organs where DCs interact with lymphocytes may be critical. These results are consistent with the finding that transgenic mice that express a dominant-negative TGF- receptor on T cells are capable of rejecting tumor challenge (47). Therefore, strategies to neutralize the tumor-derived TGF- secreted by the DC/tumor fusion vaccine may further enhance the cellular immunity of DC-primed T cells to eradicate tumors.

Because TGF- not only suppresses immunity but also inhibits tumor proliferation, blocking TGF- systemically may lead to accelerated tumor growth. Neutralization of TGF- in the microenvironment of lymphoid organs may reverse immune suppression without blocking the inhibitory effect of TGF- on tumorigenesis. This can be achieved by genetically engineering DCs to express a secreted form of the dominant-negative TGF- receptor, blocking not only tumor-derived TGF-, but also host-derived TGF- in the microenvironment.

In summary, our study demonstrates that tumor-derived immunosuppressive factors can significantly reduce the efficacy of DC-based tumor vaccines. Our results also suggest that, in addition to the use of immunostimulatory factors, the blocking of tumor-derived immunosuppressive molecules may optimize the effectiveness of DC vaccines in tumor therapy. These results have important clinical implications for DC-based cancer therapy.

	Acknowledgments

 
We thank Gwo-Hsiao Chen and Chris Kirk for technical support of the DC isolation techniques.

	Footnotes

 
¹ This study was partially supported by National Institutes of Health Grant CA87943-01 and Glaxo Institute of Digestive Health.

² Address correspondence and reprint requests to Dr. Jian-Jun Chen, Division of Gastroenterology, Department of Internal Medicine, University of Michigan Health System, 4510C Medical Science Research Building 1, 1150 West Medical Center Drive, Ann Arbor, Michigan 48109-0650. E-mail address: jjjchen{at}umich.edu

³ Abbreviations used in this paper: DC, dendritic cell; CM, complete medium; PEG, polyethylene glycol.

Received for publication July 23, 2002. Accepted for publication January 27, 2003.

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Top Abstract Introduction Materials and Methods Results Discussion References

 

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