Data Supplement for The Journal of Immunology: Volume 169, Issue 6, Page 3069
D'Avirro, et al.: Gene-Conversion-Like Sequence Transfers Between Transgenic Antibody V-Genes Are Independent of RAD54
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Supplemental Data
Fig. 1 – Sequences of PCR products from immunized mice in Fig. 4B that show transgene sequence transfers. Sequences of the R16.7 and 2B4 VDJ segments present in the VVCµ transgene are shown at top. Only those codons that exhibit mutations in cloned RT-PCR products relative to the R16.7 sequence are shown. Codon sequences are entirely shown for R16.7, only differences from the R16.7 sequence are shown for other sequences whereas identities are indicated by a dash. Differences due to sequence transfers are shown in lower-case letters whereas differences due to somatic hypermutation are shown in upper case. For each animal, sequences that appear to be related based on shared somatic hypermutations are grouped. Related sequences within an individual animal are likely derived from sister B cells that all share related sequence transfers. Thus, each group of related sequences would reflect a single sequence transfer event in one precursor cell that gives rise to a number of related sister B cells.
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