Data Supplement for The Journal of Immunology: Volume 169, Issue 6, Page 2818
Martin, et al.: Susceptibility to Psoriatic Arthritis: Influence of Activating Killer Immunoglobulin-like Receptor Genes in the Absence of Specific HLA-C Alleles
This item has the following additional materials available:
PDF File - Supplemental Table 1.
Supplemental Table 1. KIR Genotyping Primers
20-50ng DNA was amplified in 20µl containing
200µM dNTP, 100-500nM of specific primer (see
supplemental Table 1), 100nM of internal control primer,
2mM MgCl2, 67mM TRIS, 16.6mM
(NH4)2SO4, and 0.5U
Taq polymerase. Cycling was carried out in a
9700 thermal cycler (PE Applied Biosystems) under the
following conditions: 1 min at 96°C, 5cycles of
96°C for 25 s, 65°C for 45 s, 72°C for 30 s;
21 cycles of 96°C for 25 s, 60°C for 45 s,
72°C for 30 s; 5 cycles of 96°C for 25 s,
55°C for 1 min, 72°C for 2 min, followed by a
final extension step for 10 min at 72°C. PCR
products were electrophoresed in 1.5% agarose gels
containing ethidium bromide, and predicted size products
were visualized under ultraviolet light.
Prepared by: the Data Supplement Manager
