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The Journal of Immunology, 2002, 168: 5920-5927.
Copyright © 2002 by The American Association of Immunologists

The Human T Cell Response to Myelin Oligodendrocyte Glycoprotein: A Multiple Sclerosis Family-Based Study1

Niklas K. U. Koehler*, Claude P. Genain*, Barbara Giesser{dagger} and Stephen L. Hauser*

* Department of Neurology, University of California, San Francisco, CA 94143; and {dagger} Department of Neurology, University of Arizona, Tucson, AZ 85724


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Myelin oligodendrocyte glycoprotein (MOG) is an encephalitogenic myelin protein and a likely autoantigen in human multiple sclerosis (MS). In this work, we describe the fine specificity and cytokine profile of T cell clones (TCC) directed against MOG in three nuclear families, comprised of four individuals affected with MS and their HLA-identical siblings. TCC were generated from PBMC by limiting dilution against a mixture of eleven 20-mer overlapping peptides corresponding to the encephalitogenic extracellular domain of human MOG (aa 1–120). The frequency of MOG peptide-reactive T cells was surprisingly high (range, 1:400 to 1:3,000) and, unexpectedly, cloning efficiencies were highest at low seeding densities of 102 or 103 PBMC per well. A total of 235 MOG peptide-reactive TCC were produced, all of which were CD4+CD8-TCR{alpha}{beta}+TCR{gamma}{delta}-. All 11 MOG peptides were recognized by the TCC, and different epitopes of MOG appeared to be immunodominant in the HLA-identical siblings. The patterns of cytokine secretion by TCC from single individuals were generally similar. The healthy individuals exhibited Th2-, Th0-, and T regulatory cell 1-like cytokine profiles, whereas TCC from one sibling with MS had a striking Th1-like phenotype, producing high levels of IFN-{gamma} and TNF-{alpha}, and low IL-4 levels. Thus, MOG-reactive T cells appear to constitute an important part of the natural T cell repertoire, a finding that could contribute to the development of autoimmunity to this protein.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
Multiple sclerosis (MS)3 is an autoimmune disease thought to be caused by a concerted attack of T cells, B cells, and macrophages against myelin components of the CNS (1, 2). The immune responses against the quantitatively major myelin proteins, specifically myelin basic protein (MBP) and proteolipid protein, have been well characterized, both in animal models (experimental allergic encephalomyelitis) and in human MS. MBP appears to be a critical T cell autoantigen in MS (2); however, because MBP is expressed in central and peripheral nervous system myelin, additional factors must be present to explain the specificity of MS for CNS white matter. Myelin oligodendrocyte glycoprotein (MOG) is a minor myelin protein that is exclusively expressed in the CNS and is located on the extracellular membrane of oligodendrocytes, their processes, and the outermost myelin lamellae (3, 4, 5, 6, 7). In several animal species, most notably non-human primates, MOG is highly encephalitogenic and induces a primary demyelinating disease that closely mimics human MS (8, 9, 10, 11, 12, 13, 14). Large concentric areas of macrophage infiltration, autoantibody deposition, and vesicular demyelination are characteristic of MOG-induced experimental allergic encephalomyelitis (15, 16). In humans, autoimmunity against MOG also appears to play a causative role in the pathogenesis of MS. MOG-specific autoantibodies have been detected in situ in actively demyelinating MS lesions (15). High levels of reactivity of PBMC against MOG (17, 18, 19), high precursor frequencies of MOG-reactive T cells in serum and cerebrospinal fluid (CSF), and CSF autoantibodies (20, 21, 22) have also been associated with MS. In this study, a limiting dilution technique was used to generate MOG-reactive T cell clones (TCC) from haploidentical siblings belonging to MS-prone families. The fine specificity and cytokine patterns of the TCC were also analyzed. Our data indicate that MOG peptide-reactive T cells occur with surprisingly high frequency in the normal T cell repertoire and raise the possibility that individual differences exist in the response to MOG that may be relevant to the pathogenesis of MS.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Subjects

Individuals from three MS families with multiple affected members were studied (Table IGo). Family I consisted of two unaffected parents (I-Uf and I-Um), two daughters (I-Ms1 and I-Us1) discordant for MS, and two sons (I-Ms2 and I-Us2) also discordant for MS. The affected daughter (I-Ms1) was treated with IFN-{beta}-1b, while the affected son (I-Ms2) was untreated. Families II and III consisted of a sibling pair discordant for MS (II-Ms1 and II-Us1; III-Ms1 and III-Us2); in each, the affected individual was untreated (Table IGo). All affected individuals had relapsing-remitting, clinically definite MS (23). HLA typing was performed by a PCR method as previously described (23). All siblings within a given family were HLA-haploidentical.


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  		Table I. Summary of TCC reactive to MOG

 
Antigens

For T cell cloning and epitope mapping studies, a series of eleven 20-mer peptides overlapping by 10 aa and corresponding to the entire sequence of the extracellular domain of human MOG (aa 1–120) were synthesized using standard F-moc chemistry and purified >95% as verified by HPLC and mass spectrometry (Research Genetics, Huntsville, AL). In addition to the peptides, 82 positively reacting TCC from family I were tested for reactivity against recombinant proteins corresponding to the extracellular domains of recombinant human (rh)MOG and recombinant rat (rr)MOG, produced in Escherichia coli and purified as described previously (24). The purity of these recombinant proteins was confirmed by SDS-PAGE and silver staining. For 26 MOG-reactive TCC from family I, fine mapping of epitopes was performed using a panel of multiple 12-mer peptides overlapping by 1 aa (Chiron Mimotopes, San Diego, CA).

Establishment of MOG peptide-reactive TCC

PBMC were isolated from freshly drawn heparinized blood by Ficoll density centrifugation (Pharmacia Biotech, Uppsala, Sweden). PBMC were incubated at a cell density of 4 x 106/ml in AIM-V medium (Life Technologies, Gaithersburg, MD) for 3 days in a bulk culture with a mixture of the 11 synthetic MOG peptides, at a final concentration of 10 µg/ml for each peptide, using a 50-ml tissue culture flask (Nunc, Roskilde, Denmark). After 3 days, the cells were harvested by resuspending, washed twice in RPMI 1640 to remove dead cells and Ag, and counted. Viable cells were seeded in AIMV medium (Life Technologies) in the presence of rIL-2 (10 U/ml) and IL-4 (4 U/ml) (both from Life Technologies) at densities of 102, 103, 104, and 105 cells/well using 96-well round-bottom plates (Costar, Cambridge, MA). A total of 105 irradiated autologous PBMC per well were added to the three lower densities. Wells (120 or 96) were plated per dilution for each individual (Table IGo). After 3 days, 100 µl of the IL-2/IL-4-containing medium was removed, 100 µl of fresh growth medium was added, and the cells were cultured for an additional 3 days. The cells were then rested in AIM-V medium for 4–6 days, depending on their state of activation as assessed by visual inspection. APCs always consisted of freshly isolated autologous irradiated (4000 rad) PBMC that had been incubated before irradiation for at least 1 h with the MOG peptide mixture at 10 µg/ml in a 50-ml tissue culture flask at a cell density of 2 x 106/ml, at 37°C in a CO2 incubator. Two days after addition of APC to the cultured T cells, the AIM-V medium was changed with IL-2/IL-4-containing medium. Every 2 wk the T cells were restimulated. With each restimulation all wells were tested for MOG peptide reactivity by a proliferation assay. TCC were expanded in 24-well plates (Costar) or 50-ml tissue culture flasks (Nunc) by repeated restimulations and the addition of 10% T cell growth factor (Cellular Products, Buffalo, NY) to obtain the needed cell numbers for FACS analysis, epitope mapping, and cytokine analysis.

Proliferation assays

Reactivity of fresh PBMC or TCC in response to rhMOG and rrMOG, the MOG peptide mixture, and individual MOG peptides was tested in short-term proliferation assays. Either 104 T cells/well or 105 PBMC/well were plated in 96-well round-bottom plates (PBMC were plated in triplicate for each Ag). T cells received Ag pulsed or unpulsed APC (control wells) and were incubated for 48 h. As described above, APC were fresh autologous PBMC that had been pulsed with the MOG peptide mixture and then irradiated. PBMC received Ag at a concentration of 10 µg/ml or no Ag (control wells) and were incubated for 72 h. A total of 0.5 µCi [3H]thymidine was then added to each well, and after an additional 14 h the cells were harvested on a glass filter mat using a 96-well harvester (Inotech, Wohlen, Switzerland). Radioactivity was measured in a scintillation counter (Betaplate; Wallac, Turku, Finland). Ag-specific proliferation was expressed as a stimulation index, calculated as the ratio of [3H] incorporation in Ag-stimulated and unstimulated PBMC or T cells. Only MOG-containing wells with counts >500 were analyzed. A TCC well was considered as MOG peptide-reactive if at least four proliferation assays in succession showed a positive proliferative response (stimulation index, >2). The optimal concentration of Ag used in this study was established in preliminary experiments using a range of 2–100 µg/ml. Frequencies of MOG peptide-reactive TCC were calculated by dividing the number of reactive wells by the total number of PBMC plated at each dilution.

Phenotype of cells

CD4, CD8, CD45, TCR{alpha}{beta}, and TCR{gamma}{delta} expression were determined with a FACScan (BD Biosciences, Mountain View, CA), using the mAbs CD4 FITC, CD8 PE, CD45 PE-Cy5, pan-TCR{alpha}{beta} FITC, and pan-TCR{gamma}{delta} PE (all from Immunotech, Marseilles, France), according to the manufacturer’s instructions. A PE-labeled isotype control was used to exclude nonspecific binding.

Cytokine assays and analysis of cytokine patterns

Cytokine concentrations (measured in picograms per milliliter) of cell culture supernatants from 92 MOG peptide-reactive TCC from family I were determined by ELISA. The same pooled supernatant of each TCC was used in all cytokine assays. The following cytokines were measured: IFN-{gamma}, TNF-{alpha}, IL-10, IL-6, IL-4 (all from BioSource International, Camarillo, CA), and TGF-{beta}1 (R&D Systems, Minneapolis, MN). T cells were rested for 4–6 days, washed, and resuspended in AIM-V. A total of 105 T cells/well of each clone were stimulated with 5 x 105 Ag pulsed APC/well in 250 µl AIM-V/well, using a 96-well round-bottom plate. MOG peptide-pulsed APC were prepared as described above. Supernatants were harvested after 2 days and stored at -70°C until use. The ELISA were performed according to the manufacturer’s instructions. The measured cytokine concentrations were corrected against background cytokine secretion from the APC by subtracting cytokine concentrations produced from APC alone incubated with MOG peptide.

The detection limits of the cytokine kits were as follows: human IFN-{gamma}, <4 pg/ml; human TNF-{alpha}, <1 pg/ml; human IL-4, <2 pg/ml; human IL-6, <2 pg/ml; human IL-10, <5 pg/ml; and TGF-{beta}1, <5 pg/ml. Cytokine levels below the detectable concentration limit were set at that limit. Cytokine ratios were calculated by dividing IFN-{gamma} or TNF-{alpha} (both Th1 cytokines) by IL-4 (Th2 cytokine) concentrations.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
Phenotype and epitope specificity of MOG-reactive T cells

The subjects, their disease status and HLA-DR haplotype, the number of seeded wells for the four tested dilutions, the total number of generated MOG peptide-reactive TCC, the immunodominant MOG peptides, the number of TCC against the dominant peptide, and the estimated frequency of MOG peptide-reactive T cells are summarized in Table IGo. The best cloning efficiencies were observed at 102 or 103 PBMC/well. At 104 and 105 PBMC/well, the number of generated TCC gradually decreased in all examined individuals (Fig. 1Go). Thus, we observed that the initial seeding density of cells dramatically influenced the estimate by limiting dilution of their frequency in peripheral blood.



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  		FIGURE 1. Frequencies of MOG peptide-reactive TCC at the four tested seeding densities. Unexpectedly, the best cloning efficiencies were observed at 102 and 103 PBMC/well. The number of generated TCC markedly decreased with higher cell concentrations. For the individuals in family I, no TCC could be generated at 105 PBMC/well.

 
A total of 235 MOG peptide-reactive TCC were generated. IFN-{beta} appeared to inhibit the generation of reactive TCC; only four TCC were generated from patient I-Ms1 and each of these lost MOG peptide reactivity in culture. In additional experiments with PBMC from three other IFN-{beta}-treated MS patients, we were able to generate a total of only three MOG-reactive TCC in one individual from whom 120 wells were seeded at 104 cells/well (data not shown). All TCC were CD4+CD8-CD45+TCR{alpha}{beta}+TCR{gamma}{delta}- by FACS analysis.

The TCC were tested in proliferation assays for their reactivity against the individual MOG peptides, rhMOG and rrMOG. All 11 MOG peptides were recognized by the TCC. Three MOG-reactive wells from I-Uf, as well as one well each from I-Ms2 and I-Us2 seeded at 102 or 103 PBMC/well, reproducibly recognized four or more MOG peptides. Despite the identical DR/DQ haplotype of the siblings in each of the three families, the TCC from each individual revealed a distinct repertoire of reactivity against MOG peptides (Table IGo and Fig. 2Go). Dominant patterns for the haploidentical siblings can be summarized as follows: in family I, 23 of 45 (51%) TCC from I-Ms2 recognized aa 11–30, 36 of 72 (50%) TCC from I-Us2 recognized aa 21–40, and 5 of 19 (26%) TCC from I-Us1 recognized aa 31–50; in family II, 8 of 26 (31%) TCC from II-Ms1 recognized aa 71–90 and 7 of 17 TCC from II-Us1 (41%) recognized aa 1–20; and in family III, 10 of 22 (46%) TCC from III-Ms1 recognized aa 41–60 and 6 of 13 (46%) TCC from III-Us2 recognized aa 101–120. Thus, it is evident that no single linear peptide of MOG was immunodominant in peripheral blood T cells derived from HLA-identical individuals.



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  		FIGURE 2. Percentage of TCC of all MOG peptide-reactive TCC from each individual directed against single MOG peptides. Despite the haploidentical status of siblings in each of the families, a different immunodominant MOG peptide was identified in each individual.

 
The pattern of reactivity of TCC against MOG peptides was also highly sensitive to the seeding density (Fig. 3Go). For individual I-Us2, at 102 PBMC/well the TCC equally recognized aa 11–30 and 21–40; however, TCC specific to aa 11–30 were fewer if seeding of PBMC occurred at 103 cells/well, and diminished further at 104 PBMC/well. At these higher seeding densities, aa 21–40 appeared to become immunodominant. By contrast, TCC from I-Ms2 dominantly recognized aa 11–30 at all plating densities. These data highlight an additional previously unrecognized variable in T cell repertoire analysis determined by limiting dilution, namely the sensitivity of the method to the culture conditions.



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  		FIGURE 3. Percentage of TCC (of all MOG peptide-reactive TCC from each individual) from I-Ms2 and I-Us2 directed against MOG peptides aa 11–30 and aa 21–40 at the different seeding densities. The seeding density (PBMC per well), the total number of TCC at that seeding density, and the number of TCC to aa 11–30 and 21–40 at that density are shown. TCC from the healthy individual I-Us2, generated at 102 PBMC/well, reacted equally against aa 11–30 and 21–40; however, at higher seeding densities the TCC predominantly recognized aa 21–40. In contrast, TCC from the affected brother I-Ms2 tended to react against aa 21–40 at all densities.

 
The epitope specificities of 26 TCC from the three haploidentical siblings in family I, reactive to MOG peptides aa 11–30, 31–40, and 31–50, were more finely mapped in proliferation assays using individual 12-mer MOG peptides overlapping by 1 aa each (Fig. 4Go). Sixteen TCC from I-Ms2 and I-Us2, specific to aa 11–30, exhibited identical peptide reactivity, delineating a fine specificity to aa 13–24. Seven TCC from I-Us2 and two TCC from I-Usl, reactive to aa 21–40, had fine specificities to aa 30–40 and aa 23–32, respectively. One TCC from I-Us1, reactive to aa 31–50, had a fine specificity to aa 36–46. Thus, immunodominant responses to some MOG epitopes with identical fine specificity could be identified both within individuals and across some, but not all, HLA-identical subjects.



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  		FIGURE 4. Fine epitope mapping of TCC reactive to the MOG peptides aa 11–30, 21–40, and 31–50, using synthetic 12-mer MOG peptides overlapping by 1 aa. TCC of the DR/DQ haploidentical siblings from family I, I-Ms2, I-Us1, and I-Us2, were analyzed. Reactive MOG peptides are shaded, and suitable target epitopes are framed. TCC specific to aa 11–30 from I-Ms2 and I-Us2, and TCC specific to aa 21–40 from I-Us2, delineated identical fine specificities to aa 13–24 and 30–40, respectively. Numbers at the left identify individual overlapping peptides.

 
Not all MOG peptide-reactive TCC were also reactive against the extracellular domain of whole MOG. A total of 82 TCC from family I were tested for their reactivity to rhMOG and rrMOG. Of these, 37 TCC (45%) showed a positive proliferative response to rhMOG and 43 TCC (52%) showed a positive proliferative response to rrMOG. The frequency of peptide-reactive clones that also recognized rhMOG was variable: 12 of 20 TCC (60%) from I-Ms2 and I-Us2, specific to MOG aa 11–30, and 3 of 4 TCC (75%) from I-Us1 specific to aa 31–50; however, only 1 of 13 TCC (8%) from I-Us2 reactive to MOG aa 21–40 and 1 of 5 TCC (20%) from I-Uf specific to aa 71–90 reacted to rhMOG.

No primary proliferative responses to MOG were detected in any subject when freshly isolated PBMC were plated at 105 cells/well and cultured in the presence of the MOG peptide mixture, rhMOG, or rrMOG in triplicate short-term (86-h) proliferation assays (data not shown).

Cytokine production

None of the MOG peptide-reactive TCC secreted TGF-{beta} above background levels produced by the APC. Besides TGF-{beta}, only IL-6 was produced in low amounts by the APC, and in all analyses IL-6 production of the TCC was corrected against background levels of secretion by APC alone. Cytokine concentrations (measured in picograms per milliliter) for TNF-{alpha}, IFN-{gamma}, IL-4, IL-6, and IL-10 in supernatants of individual MOG peptide-reactive TCC are illustrated in Fig. 5Go, and Th1 (IFN-{gamma}, TNF-{alpha}):Th2 (IL-4) ratios are shown in Fig. 6Go.



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  		FIGURE 5. Cytokine concentrations in supernatants of the MOG-reactive TCC from family I. TCC specific to aa 11–30 (lightly shaded) and 21–40 (darkly shaded) are indicated. High levels of Th1 cytokines were secreted by TCC from MS patient I-Ms2; by contrast, TCC from the healthy individuals I-Us2, I-Us1, and I-Uf, had Th2-, Th0-, and Tr1-like phenotypes, respectively. *, Cytokine level below the detection limit of the assay (see Materials and Methods for details).

 


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  		FIGURE 6. TNF-{alpha}:IL-4 and IFN-{gamma}:IL-4 ratios of MOG peptide-reactive TCC from family I. TCC specific to aa 11–30 (lightly shaded) and aa 21–40 (darkly shaded) are indicated. A strong Th1-like cytokine pattern is apparent for the TCC derived from patient I-Ms2.

 
In this analysis, IFN-{gamma} and TNF-{alpha}{beta} were considered to be produced by Th1 cells, IL-4 by Th2 cells, and IL-6 and IL-10 by both subsets, but at lower levels by Th1 cells (25, 26). Th0 cells produce both IL-4 and IFN-{gamma}; TGF-{beta} has been ascribed to Th3 regulatory cells (27) and IL-10 to T regulatory cell 1 (Tr1) T cells (28, 29, 30). MOG peptide-reactive TCC from family I exhibited Th1-, Th2-, Th0-, and Tr1-like cytokine profiles. Compared with the unaffected individuals, TCC from patient I-Ms2 exhibited a striking Th1-like phenotype, with very high TNF-{alpha} levels, high levels of IFN-{gamma} and IL-6, and very low IL-4 levels. TCC from I-Us2 produced a Th2-like cytokine pattern with high levels of IL-4 and IL-6 and low levels of IFN-{gamma} and TNF-{alpha}. TCC from I-Us1 exhibited a Th0-like pattern, secreting high levels of IFN-{gamma}, IL-4, and IL-10, but low levels of TNF-{alpha} and IL-6. TCC from I-Uf had a Tr1-like cytokine profile, producing high levels of IL-10, but otherwise low amounts of all other cytokines. Thus, the cytokine production of MOG-specific TCC differed between individuals, and TCC generated from a single individual tended to display a homogeneous cytokine profile. To determine whether the cytokine profiles of MOG peptide-reactive TCC were stable in vitro, the levels of IFN-{gamma} and IL-4 were measured serially from 16 TCC from I-Us1 and I-Uf. For each TCC, four measurements were obtained over a 10-wk period, beginning with the second and ending with the fifth restimulation. No shift in cytokine profile was observed for any of the clones (data not shown).


   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
These studies were undertaken because of emerging data implicating MOG, a CNS-specific myelin protein, as an autoantigen in human MS (15). We found that the frequency of TCC against MOG peptides was remarkably high, up to 1:444 in the healthy sibling I-Us2. Notably, the best cloning efficiencies were observed at the lowest seeding densities, e.g., 102 or 103 PBMC/well. These data are similar to our analysis of the characteristics of the circulating T cell repertoire to MOG in naive, unimmunized marmosets (46). Taken together, these findings clearly demonstrate that MOG-reactive T cells are highly prevalent in the peripheral T cell compartment. Although no individual in the current study demonstrated reactivity to rMOG in short-term proliferation assays of freshly isolated PBMC, previous studies have demonstrated a high level of proliferation against MOG in some MS patients (17, 18) and a high precursor T cell frequency in peripheral blood and CSF (1:7299 and 1:450, respectively) enumerated by measuring IFN-{gamma} in response to stimulation with MOG (20, 22). However, in our experience, proliferative responses of whole PBMC to rhMOG or rrMOG are equally prevalent in MS and control individuals (19).

In contrast to MOG, relatively low frequencies of MBP-reactive T cells, generally in the range of 10-5–10-6, have been described by traditional limiting dilution methods in MS patients (31, 32, 68). A similar low frequency has been reported against another quantitatively major myelin protein, proteolipid protein (32). Using different approaches, such as those based upon detection of a known MBP-specific TCR chain or by cloning in the presence of exogenous IL-2, much higher estimates of MBP-reactive T cells, in the range of 10-2–10-3, have been reported (33, 34). The use of synthetic peptides, in contrast to recombinant or native protein, is known to further increase both the number and diversity of generated MBP-reactive TCC (35, 36, 37).

In the normal repertoire, the finding of a high frequency of T cells reactive against some myelin Ags suggests that homeostatic mechanisms must be active to prevent autoimmune demyelination in the absence of provoking triggers. It is well established that MBP- and MOG-reactive T cells present in naive animals are potentially encephalitogenic following activation in vitro and adoptive transfer (38, 39, 40). The regulatory mechanisms responsible for containment of autoreactive T cells are not fully defined; however, possibilities include anti-idiotypic suppressor cells (41, 42, 43, 44) and self-MHC-reactive T cells (28, 29, 30). Anti-idiotypic suppressor cells were found to contribute to prevention of spontaneous autoimmunity in double-transgenic mice engineered to express encephalitogenic TCR molecules (44). Self-MHC-reactive T cells proliferated together with Ag-specific T cells following stimulation with tetanus toxin in vitro to display a regulatory (Tr1) cytokine phenotype producing IL-10 and to suppress T cell proliferation and Ig production (28, 29, 30). These cells appear to form an important part of the T cell repertoire and have been found at frequencies >1:50 by single T cell cloning in a healthy human subject (45). Lower estimates of MOG-reactive T cells at higher seeding densities might be explained by the presence of MHC-reactive cells that suppressed the growth of MOG-reactive T cells when cultured at high cell concentrations. The presence of such regulatory T cell populations in the peripheral blood of healthy individuals could provide an effective means to control expansion of MOG-reactive T cells.

Little is known of the fine specificity of the T cell response to MOG in humans or in patients with MS. In this study, all 11 MOG peptides were recognized by the TCC, and different peptides were found to be immunodominant in HLA-identical siblings. Observed epitope dominance was also critically influenced by the initial seeding density of PBMC. As the MHC and exogenous events are generally considered to be the principal factors that shape the T cell repertoire, the current in vitro observations highlight the additional importance of the immunological microenvironment as a determinant of the repertoire.

Some of the predominant epitopes of MOG detected in this study contain peptides that experimentally have been shown to be encephalitogenic (46, 47, 48, 49, 50, 69, 70). These findings underscore the potential importance of TCC specific to aa 11–30 (aa 13–24), which also exhibited a strong Th1-like cytokine profile in the patient I-Ms2.

Notably, a diverse repertoire of epitopes within the extracellular domain of MOG were recognized by TCC, both within individuals and between HLA-identical siblings. In rodents with acute autoimmune disease, the repertoire of pathogenic autoreactive T cells may recognize an extremely limited array of protein epitopes to the immunizing Ag or tissue, and determinant spreading to a wider range of epitopes may develop over time (51, 52, 53). In humans, as in other outbred species (46), the response to large protein Ags appears to be far more diverse than in rodents, although dominant epitopes may be identified in association with some HLA haplotypes (54). Even in monozygotic twins, the allospecific T cell repertoire was found to differ between individuals (55, 56). Whereas heterozygosity at MHC loci is one likely contributor to diversity of epitope recognition in outbred individuals, these findings strongly suggest that epigenetic factors represent the predominant influence on the circulating T cell repertoire.

Even if the frequency and epitope specificity of autoreactive T cells are similar between individuals with MS and controls, it is possible that their patterns of cytokine secretion differ. In rodents, the production of TNF-{alpha} and lymphotoxin correlated with encephalogenicity of MBP-specific TCC (57). High levels of TNF-{alpha} have been identified in MS brain lesions (58), and some reports indicate that levels of Th1 cytokines produced by PBMC (59, 60, 61, 62), by CSF (63), or by myelin-reactive TCC or lines (35, 64) have also been associated with disease activity in MS. One analysis of cytokine secretion of 30 MBP-specific TCC revealed elevated IFN-{gamma}:IL-4 ratios in MS patients (65). By contrast, other studies failed to demonstrate clear differences in cytokine profiles of MBP-reactive TCC or T cell lines between patients and controls (66, 71).

We found that many MOG peptide-reactive TCC exhibited cytokine profiles with different admixtures of Th1- and Th2-type cytokines, supporting the concept that a strict separation of T cells in the Th1 and Th2 phenotype may not be applicable to humans (65, 72). The cytokine production of TCC differed between the individuals, but TCC derived from one individual exhibited a rather homogenous cytokine profile. We observed Th2-, Th0-, and Tr1-like cytokine patterns in the healthy individuals and a striking Th1-like cytokine profile in the affected sibling I-Ms2. Longitudinal analysis of IFN-{gamma}:IL-4 ratios of individual MOG peptide-reactive TCC during the culture period indicated that the cytokine profiles of the TCC in vitro remained stable. Finally, no correlation between the cytokine patterns and epitope specificities of MOG-reactive TCC could be identified, analogous to previous observations of MBP-specific TCC.

In summary, we provide evidence that MOG peptide-reactive T cells occur with extraordinary high frequency, both in MS patients and in their healthy siblings. Therapy with IFN-{beta} appeared to reduce the number of MOG peptide-reactive T cells that could be generated. We also show that the dominant epitopes recognized by MOG peptide-reactive TCC differ in HLA-identical siblings, suggesting that epigenetic factors, or non-HLA genes, play a major role in shaping the T cell repertoire to this Ag. Although these results are consistent with a possible role for T cell responses directed against MOG in the pathogenesis of MS, no clear disease-specific changes in the T cell frequency or repertoire could be identified. This conclusion is similar to that reached by Lindert et al. (67) in a study of five unrelated MS patients and controls who were not HLA-matched. However, a striking Th1-like cytokine profile was present in one of our MS patients, leaving open the possibility that an enhanced proinflammatory T cell response, directed against MOG, may be present in a subset of MS patients and could correlate with the presence of MOG-mediated tissue damage in situ.


   Acknowledgments
 
We thank Scott Mayfield and Janeen Islar for technical assistance. We also acknowledge, with special thanks, the individuals who generously donated blood for these studies and endured the discomfort of repeated phlebotomies.


   Footnotes
 
1 N.K.U.K. was a postdoctoral fellow of the Deutsche Forschungsgemeinschaft (KO 1719/1-1). C.P.G. was a Harry Weaver Neuroscience Scholar of the National Multiple Sclerosis Society. Back

2 Address correspondence and reprint requests to Dr. Stephen L. Hauser, Department of Neurology, University of California, San Francisco, CA 94143. E-mail address: hauser{at}itsa.ucsf.edu Back

3 Abbreviations used in this paper: MS, multiple sclerosis; TCC, T cell clone; MBP, myelin basic protein; MOG, myelin oligodendrocyte glycoprotein; rh, recombinant human; rr, recombinant rat; Tr1, T regulatory cell 1; CSF, cerebrospinal fluid. Back

Received for publication April 16, 2001. Accepted for publication March 25, 2002.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

  1. Steinman, L.. 1996. Multiple sclerosis: a coordinated immunological attack against myelin in the central nervous system. Cell 85:299.[Medline]
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