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,
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Departments of
*
Pathology,
Obstetrics and Gynecology,
Molecular Microbiology and Immunology, and
Oncology, Johns Hopkins Medical Institutions, Baltimore, MD 21205; and
¶ Department of Obstetrics and Gynecology, National Taiwan University Hospital, National Taiwan University, Taipei, Taiwan
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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One potential approach to enhance the spread of Ag is to use VP22, an HSV-1 protein that has demonstrated the remarkable property of intercellular transport and is capable of distributing protein to many surrounding cells (4). For example, VP22 has been linked to p53 (5) or thymidine kinase (6), facilitating the spread of linked protein to surrounding cells in vitro and the treatment of model tumors. Therefore, we investigated the novel use of VP22 linked to a model Ag, human papillomavirus (HPV)4 type 16 E7, in the context of a DNA vaccine and explored whether it led to the spread of linked Ag to surrounding cells and enhanced Ag-specific immune responses and antitumor effects.
We demonstrate that the linkage of VP22 to E7 led to a dramatic increase in the number of E7-specific CD8+ T cell precursors in vaccinated mice (around 50-fold) and converted a less effective DNA vaccine into one with significant potency against E7-expressing tumors. Furthermore, experiments with a nonspreading VP221–267 mutant failed to enhance vaccine potency, indicating that the property of intercellular spreading generated by VP22 was important for enhancing vaccine potency. These data indicated that the strategy of linking VP22 to a model Ag can dramatically improve the potency of naked DNA vaccines.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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We used pcDNA3 as an expression vector instead of a previously described pCMV-Neo-Bam vector (7, 8) that generates a stronger vector-related antitumor effect. For the generation of pcDNA3-VP22, VP22 was subcloned from pVP22/myc-His (Invitrogen, Carlsbad, CA) into the unique EcoRV and BamHI cloning sites of the pcDNA3.1(-) expression vector (Invitrogen) downstream of the cytomegalovirus promoter. The generation of pcDNA3-E7 has been described previously (9). For the generation of pcDNA3-VP22/E7, VP22 was subcloned from pcDNA3-VP22 into the unique EcoRV and BamHI cloning sites of the pcDNA3-E7. For the generation of pcDNA3-E7(E/B), which contains E7 with EcoRI and BamHI restriction sites on the flanking ends of E7, we first used PCR to amplify the E7 fragment with pcDNA3-E7 and a set of primers: 5'-ggggaattcatggagatacaccta-3' and 5'-ggtggatccttgagaacagatgg-3'. The amplified product was further cloned into the EcoRI/BamHI sites of pcDNA3. For the generation of pcDNA3-VP221–267/E7, a DNA fragment encoding VP221–267 was first amplified by PCR with pcDNA3-VP22 and a set of primers: 5'-gggtctagaatgacctctcgccgctccgt-3'and 5'-ggggaattcgtcctgcaccacgtctggat-3'. The amplified product was cloned into the XbaI/EcoRI cloning sites of pcDNA3-E7(E/B).
For the generation of pcDNA3-green fluorescent protein (GFP), a DNA fragment encoding GFP first was amplified by PCR with pEGFPN1 DNA (Clontech, Palo Alto, CA) and a set of primers: 5'-atcggatccatggtgagcaagggcgaggag-3'and 5'-gggaagctttacttgtacagctcgtccatg-3'. The amplified product was cloned into the BamHI/HindIII cloning sites of pcDNA3. For the generation of pcDNA3-VP22/GFP, VP22 was subcloned from pcDNA3-VP22 into the unique EcoRV and BamHI cloning sites of the pcDNA3-GFP. For construction of pcDNA3-E7/GFP, GFP was isolated from pcDNA3-GFP and cloned into BamHI/HindIII sites of pcDNA3-E7(E/B). For construction of VP22/E7/GFP, VP22 was amplified by PCR with pcDNA3-VP22 and a set of primers: 5'-gggtctagaatgacctctcgccgctccgt-3' and 5'-ggggaattcctcgacgggccgtctggggc-3', then cloned into XbaI/EcoRI sites of pcDNA3-E7/GFP. For construction of pcDNA3-VP221–267/E7/GFP, VP221–267 was isolated from pcDNA3-VP221–267 and cloned into XbaI/EcoRI sites of pcDNA3-E7/GFP.
For the generation of vaccinia, we used a protocol similar to one described previously (10). The generation of pSC11-E7 has been described previously (10). To generate pSC11-VP22/E7, VP22 was isolated from pcDNA3-VP22/E7 by NotI/Pmel and cloned into NotI/SmaI sites of pSC11 vector. To generate pSC11-VP22, VP22 was isolated from pcDNA3-VP22 by NotI/PmeI and cloned into NotI/SmaI sites of pSC11 vector.
For the generation of pcDNA3-TAT/E7, we synthesized complementary oligomers encoding MRKKRRQRRR (11, 12): 5'-ctagaatgtacggccgcaagaaacgccgccagcgccgccgcg-3' and 5'-aattcgcggcggcgctggcggcgtttcttgcggccgtacatt-3'. The oligomers were annealed and cloned into the XbaI/EcoRI sites of pcDNA3-E7(E/B). For the generation of pcDNA3-E7/MTS, we synthesized complementary oligomers encoding AAVLLPVLLAAP (13): 5'-gatccgcagccgttcttctccctgttcttcttgccgcacccta-3' and 5'-agcttagggtgcggcaagaagaacagggagaagaacggctgcg-3'. The oligomers were annealed and cloned into the BamHI/HindIII sites of pcDNA3-E7(E/B). For the generation of pcDNA3-AH/E7, we synthesized complementary oligomers encoding MRQIKIWFQNRRMKWKK (14): 5'-ctagaatgcgccaaatcaaaatctggttccagaatcgacgaatgaagtggaaaaaag-3' and 5'-aattcttttttccacttcattcgtcgattctggaaccagattttgatttggcgcatt-3'. The oligomers were annealed and cloned into the XbaI/EcoRI sites of pcDNA3-E7(E/B). The accuracy of all the DNA constructs was confirmed by sequencing.
DNA vaccination
Preparation of DNA-coated gold particles and gene gun particle-mediated DNA vaccination was performed with a helium-driven gene gun (Bio-Rad, Hercules, CA) according to a previously described protocol (9). DNA-coated gold particles (1 µg DNA/bullet) were delivered to the shaved abdominal region of C57BL/6 mice with a helium-driven gene gun (Bio-Rad) with a discharge pressure of 400 p.s.i.
Fluorescent microscopic examination
293 Db,Kb cells were used for an in vitro assay of GFP expression. 293 Db,Kb cells were provided kindly by Dr. J. C. Yang (National Cancer Institute, National Institutes of Health, Bethesda MD) (15). A total of 20 µg of E7/GFP, VP22/E7/GFP, or VP221–267/E7/GFP DNA was transfected into 5 x 106 293 Db,Kb cells with lipofectamine 2000 (Life Technologies, Rockville, MD). Cells were collected 48 h after transfection and fixed with 100% methanol for 10 min at room temperature. The glass slides were mounted with anti-fading medium, Mowiol 4-88 (Calbiochem, La Jolla, CA) and covered with coverslips. Samples were examined on a fluorescent microscope.
Immunohistochemical staining
Vaccinated mice were sacrificed 3 days after the pcDNA3-VP22/GFP or pcDNA3-GFP DNA vaccination. Skin was biopsied, fixed, embedded in paraffin, and cut into 5-µm sections. After deparaffinization and hydration, the slides were incubated with rabbit anti-GFP polyclonal Ab (1:200 dilution; Molecular Probes, Eugene, OR) followed by biotinylated goat anti-rabbit IgG (1:200 dilution) and avidin-biotin complex (1:100 dilution; Vector, Burlingame, CA). The slides were developed by adding diaminobenzidine substrate solution (Dako, Carpinteria, CA) and counterstained with Mayer’s hematoxylin. The stained slides then were dehydrated, mounted, and observed under a light microscope.
Intracellular cytokine staining
Cell surface marker staining of CD8 or CD4 and intracellular
cytokine staining for IFN-
and IL-4 as well as FACScan analysis were
performed under conditions described previously (9).
Before FACScan, splenocytes from naive or vaccinated groups of mice
were incubated for 20 h with either 1 µg/ml of E7 peptide (aa
49–57; Ref. 16) containing an MHC class I epitope for
detecting E7-specific CD8+ T cell precursors or
10 µg/ml of E7 peptide (aa 30–67; Ref. 17) containing
an MHC class II epitope for detecting E7-specific
CD4+ T cell precursors.
CTL assays
Splenocytes from vaccinated mice were harvested 2 wk after vaccination and cultured with E7 peptide (aa 49–57) containing an MHC class I epitope (16) at a concentration of 1 µg/ml in a total volume of 2 ml of RPMI 1640, supplemented with 10% (v/v) FBS, 50 U/ml penicillin and streptomycin, 2 mM L-glutamine, 1 mM sodium pyruvate, and 2 mM nonessential amino acids in a 24-well tissue culture plate for 6 days. Splenocytes served as effector cells, and TC-1 tumor cells (18) were used as target cells. CTL assays were performed in 96-well round-bottom plates as described by Corr et al. (19). Cytolysis was determined by quantitative measurements of lactate dehydrogenase (LDH) (19). CTL assays were performed with effector cells and targets cells (1 x 104 per well) mixed together at various ratios (1:1, 5:1, 15:1, and 45:1) in a final volume of 200 µl. After a 5-h incubation at 37°C, 50 µl of the cultured medium was collected to assess the amount of LDH in the cultured medium according to the manufacturer’s protocol of the CytoTox assay kits (Promega, Madison, WI). The percentage of specific lysis was calculated from the following equation: 100 x [(A - B)/(C - D)], where A is the reading of experimental-effector signal value, B is the effector spontaneous background signal value, C is maximum signal value from target cells, and D is the target spontaneous background signal value.
CTL assays with DNA-transfected or vaccinia-infected 293 Db,Kb cells as target cells
A total of 20 µg of pcDNA3 (no insert), VP22, E7, or VP22/E7 DNA were transfected into 5 x 106 293 Db,Kb cells with lipofectamine 2000 (Life Technologies). Transfected 293 Db,Kb cells were used as target cells, and an E7-specific CD8+ T cell line (20) served as effector cells. Untransfected 293 Db,Kb cells were used as a negative control. Cells were collected 16 h after transfection. Cytolysis was determined by quantitative measurements of LDH as described above. CTL assays were performed with effector cells and target cells (1 x 104 per well) mixed together at various ratios (1:1, 3:1, 9:1, and 27:1) in a final volume of 200 µl. After a 5-h incubation at 37°C, 50 µl of the cultured medium were collected to assess the amount of LDH in the cultured medium. The percentage of lysis was calculated as described above.
In a second CTL assay, vaccinia-infected 293 Db,Kb cells served as target cells. Briefly, 1 x 107 PFU of wild-type, VP22, E7, or VP22/E7 vaccinia was used to infect 1 x 106 293 Db,Kb cells. Cells were collected 16 h after infection. Cytolysis was determined by quantitative measurements of LDH as described above. CTL assays were performed with effector cells and targets cells (1 x 104 per well) mixed together at various ratios (1:1, 5:1, 15:1, and 45:1) in a final volume of 200 µl. After a 5-h incubation at 37°C, 50 µl of the cultured medium were collected to assess the amount of LDH in the cultured medium. The percentage of specific lysis was calculated as described above.
In vivo tumor protection experiment
For the tumor protection experiment, mice (five per group) were vaccinated via gene gun with 2 µg of pcDNA3 (no insert), VP22, E7, VP221–267/E7, or VP22/E7 DNA. One week later, mice were boosted with the same regimen as the first vaccination. One week after the last vaccination, mice were s.c. challenged with 5 x 104 cells/mouse TC-1 tumor cells in the right leg and then monitored twice a week.
In vivo tumor treatment experiment
Tumor cells and DNA vaccines were prepared as described earlier. Mice (five per group) were i.v. challenged with 1 x 104 cells/mouse TC-1 tumor cells via tail vein on day 0. Three days after challenge with TC-1 tumor cells, mice were administered 2 µg of pcDNA3 (no insert), VP22, E7, or VP22/E7 DNA via gene gun. One week later, these mice were boosted with the same regimen as the first vaccination. Mice were sacrificed and their lungs were explanted on day 21 after tumor challenge. The pulmonary nodules in each mouse were evaluated and counted by experimenters blinded to sample identity. Compared with the percentage of tumor-free mice in the s.c. tumor model, the mean number of tumor nodules in the pulmonary tumor model is a more sensitive indicator of the antitumor effect generated by each vaccine. The pulmonary tumor model may allow us to evaluate subtle differences in the antitumor effects that might not be appreciable by using our s.c. tumor model.
In vivo Ab depletion experiment
In vivo Ab depletions have been described previously (18). Briefly, mice were vaccinated with 2 µg VP22/E7 DNA via gene gun, boosted 1 wk later, and challenged with 5 x 104 cells/mouse TC-1 tumor cells s.c. Depletions were started 1 wk before tumor challenge. mAb GK1.5 was used for CD4 depletion, mAb 2.43 was used for CD8 depletion, and mAb PK136 was used for NK1.1 depletion. Depletion was terminated on day 63 after tumor challenge.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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A).
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B, a widespread distribution of positive
brown-staining was observed in the epidermis and skin appendices from
mice vaccinated with pcDNA3-VP22/GFP, with some positive staining of
nuclei in the epidermis. In contrast, only a few positive cells were
observed in the epidermis of pcDNA3-GFP-vaccinated mice. These results
suggested that the linkage of VP22 to protein led to enhanced
intercellular spreading of the linked protein. The observed increase in intercellular spreading of the marker protein within the epidermis raises the possibility of generating an increased number of APCs that present the linked protein because the epidermis is rich with Langerhans’ cells, the professional APC precursors. To further investigate whether such increased spreading can lead to enhanced Ag-specific T cell activities, we linked VP22 to a model Ag, HPV-16 E7, which is associated with a majority of cervical cancers. E7 is important in the induction and maintenance of cellular transformation and is coexpressed in most HPV-containing cervical cancers and their precursor lesions; therefore, it represents an ideal target for vaccine development (21).
The importance of CD8+ CTLs for the control of
viral infections and neoplasms has been demonstrated in several
preclinical models (9, 22). To determine whether
vaccination of mice with the pcDNA3-VP22/E7 DNA vaccine can enhance the
number of E7-specific CD8+ T cell precursors, we
performed intracellular cytokine staining on splenocytes from
vaccinated mice. As shown in Fig. 2A, mice vaccinated with
VP22/E7 DNA exhibited around a 50-fold increase in E7-specific
IFN-+ CD8+ T cell
precursors (576/3 x 105 splenocytes)
compared with mice vaccinated with wild-type E7 DNA (12/3 x
105 splenocytes). These results indicated that
the addition of VP22 to E7 significantly enhanced E7-specific
CD8+ T cell-mediated immune responses and that
fusion of E7 to VP22 was essential for this observed enhancement
because VP22 mixed with E7 (VP22 + E7 DNA, 14/3 x
105 splenocytes) did not generate a significant
increase in the number of CD8+ T cell precursors.
Furthermore, the linkage of irrelevant proteins (such as GFP) to E7 did
not generate enhancement of E7-specific CD8+ T
cell activity (data not shown).
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A, linkage
of the mutant VP221–267 to E7 failed to
significantly enhance the number of E7-specific
CD8+ T cell precursors in vaccinated mice
(17/3 x 105 splenocytes). These results
suggested that the property of intercellular spreading generated by
full-length VP22 was important for increasing the number of E7-specific
CD8+ T cell precursors.
Although addition of VP22 to E7 led to enhanced E7-specific
CD8+ T cell activities, we did not detect a
significant difference in the number of E7-specific IFN--secreting
CD4+ T cells (Fig. 2B) or
IL-4-secreting CD4+ T cells (data not shown)
among the various vaccination groups. By direct ELISA, we also detected
no significant difference in E7-specific Ab responses in the sera of
mice vaccinated with various DNA vaccines (data not shown).
Because CD8+ CTL activity has been implicated as
important for generating an antitumor effect, we used CTL assays to
study E7-specific CTL killing generated in mice vaccinated with various
DNA vaccines. We used splenocytes from mice vaccinated with various DNA
vaccines as effector cells and E7-expressing TC-1 tumor cells as target
cells. We observed the highest CTL activity in mice vaccinated with
VP22/E7 compared with mice vaccinated with pcDNA3 (no insert), VP22, or
E7 DNA (Fig. 3). Our data indicated that
CTL activity correlated with the frequency of E7-specific
CD8+ T cell precursors generated by each DNA
vaccine.
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A, 293
Db,Kb cells transfected
with VP22/E7 DNA displayed significantly higher percentages of specific
lysis at the 9:1 (22.8 ± 1.8% vs 6.2 ± 1.5%;
p < 0.001) and 27:1 (48.1 ± 2.4% vs 13.0
± 1.6%; p < 0.001) E:T ratios compared with cells
transfected with wild-type E7 DNA. Because intercellular spreading of
VP22/E7 may affect the results of this assay by generating more
E7-positive target cells, we performed a CTL assay with
vaccinia-infected target cells to alleviate this concern. Vaccinia have
been shown to infect nearly 100% of target cells. We used 293
Db,Kb cells infected with
wild-type E7, VP22, or VP22/E7 vaccinia as target cells. Our results
demonstrated that cells infected with VP22/E7 vaccinia generated a
higher percentage of lysis compared with cells infected with other
vaccinia (Fig. 4B). Taken together, our data from Fig. 4, A and B suggested that cells expressing VP22/E7
may present E7 Ag through the MHC class I pathway more efficiently than
cells expressing wild-type E7.
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A, 100% of mice receiving
the VP22/E7 DNA vaccine remained tumor-free 63 days after TC-1
challenge. In contrast, all of the unvaccinated mice and mice receiving
pcDNA3 (no insert), VP22, or wild-type E7 developed tumors within 14
days after tumor challenge. We also observed that fusion of E7 to VP22
was required for antitumor immunity, because VP22 mixed with E7 (VP22 +
E7 DNA) did not generate enhancement of tumor protection. Furthermore,
the linkage of the mutant VP221–267 to E7 failed
to generate a significant antitumor effect in vaccinated mice (Fig. 5A). These data suggested that the property of intercellular
spreading generated by linkage of full-length VP22 to E7 was important
for the observed enhancement of E7-specific tumor protection.
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B, mice vaccinated with VP22/E7 DNA exhibited the
lowest mean number of pulmonary nodules (0.75 ± 0.95) compared
with mice vaccinated with wild-type E7 DNA (32.6 ± 2.5) or VP22
DNA (13 ± 2.58; one-way ANOVA, p < 0.001).
Interestingly, vaccination with VP22 DNA generated a weak nonspecific
antitumor effect in the lung metastasis model. The results from the
tumor protection and treatment experiments indicated that linkage of
VP22 to E7 dramatically enhanced antitumor effects against the growth
of TC-1 tumors.
To determine the subset of lymphocytes that are important for the
antitumor effect generated by VP22/E7 DNA vaccine, we performed in vivo
Ab depletion experiments. As shown in Fig. 5C, all naive
mice and VP22/E7 DNA-vaccinated mice depleted of
CD8+ T cells grew tumors within 14 days after
tumor challenge. In contrast, all of the nondepleted mice and all of
the mice depleted of CD4+ T cells remained
tumor-free 63 days after tumor challenge, and 40% of NK1.1-depleted
mice grew tumors 6 wk after tumor injections. These results suggested
that CD8+ T cells but not
CD4+ T cells are essential for the antitumor
immunity generated by the VP22/E7 DNA vaccine. NK1.1 cells may also
contribute to the antitumor effect generated by the chimeric VP22/E7
DNA vaccine.
The success of the VP22/E7 DNA vaccine warrants the consideration of strategies that use proteins with trafficking properties. For example, HIV TAT protein (TAT) has been shown to have transcellular transport properties (11, 12). In addition, a peptide containing nine amino acids of TAT (RKKRRQRRR) conjugated with OVA was able to generate higher CTL activity in mice that were vaccinated with TAT-OVA compared with mice vaccinated with OVA (23). Another molecule with similar properties is the membrane-translocating sequence (MTS), which also has been shown to have transmembrane permeability. The amino acid residues accounting for this property have been narrowed down to 12 amino acids (AAVLLPVLLAAP; Ref. 13). Finally, the third helix of the Antennapedia homeodomain (AH) of the Drosophila also has been shown to have membrane translocation properties, and 16 amino acid residues have been shown to account for this effect (RQIKIWFQNRRMKWKK; Ref. 14). These studies suggest that chimeric proteins with these molecules also may result in an effect similar to that observed with VP22/E7.
Therefore, we have chimerically linked these molecules to E7 in a DNA
format and vaccinated mice with each construct to examine the
E7-specific immune responses generated by these vaccines in comparison
to VP22/E7 DNA. We performed intracellular cytokine staining for
E7-specific CD8+ T cell precursors with
splenocytes from vaccinated mice. As shown in Fig. 6, the linkage of the DNA sequence
encoding the characterized membrane translocation region of each of
these proteins to E7 did not generate as dramatic an enhancement of
E7-specific CD8+ T cell immune responses as we
had observed with VP22 linked to E7. These results indicated that
VP22/E7 DNA may have an unique property that is not shared with the
other constructs that we tested.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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In this study, we observed a dramatic increase in the number of
E7-specific CD8+ T cell precursors in mice
vaccinated with VP22/E7 (Fig. 2). One potential explanation for this
enhancement is that intradermal administration of VP22/E7 DNA can
introduce DNA directly into professional APCs of the skin
(24), allowing transfected APCs to directly present E7
through the MHC class I pathway. Another important reason for this
observed enhancement is that the linkage VP22 to E7 may facilitate the
spreading of Ag from VP22/E7 DNA-transfected cells to surrounding APCs,
increasing the number of APCs that present E7 through MHC class I
pathway. Finally, our data indicated that the linkage of VP22 to E7 is
capable of presenting the Ag more efficiently in transfected cells. All
of these factors may have played a role in enhancing the number of
E7-specific CD8+ T cell precursors observed in
vaccinated mice.
Another potential explanation for the observed enhancement of E7-specific CD8+ T cell immune responses in vivo may be the so-called "cross-priming effect", whereby release of chimeric VP22/E7 protein may lead to uptake and processing by dendritic cells (DCs) for presentation via the MHC-I restricted pathway (25). Previously, we observed that DCs pulsed with apoptotic cells expressing VP22/E7 (26) or cell lysates containing VP22/E7 fusion protein (C.-F.H., unpublished observation) were capable of presenting E7 Ag through the MHC class I pathway more efficiently than wild-type E7 counterparts. However, other studies have suggested that direct priming of CD8+ T cells by gene-transfected DCs may be a more important mechanism in gene gun-mediated DNA immunization than the cross-priming mechanism (27, 28). Thus, the extent of cross-priming in contributing to CD8+ T cell-mediated immune responses remains unclear and requires additional experiments to characterize its role in the observed enhancement of Ag-specific CD8+ T cell activity.
The property of intercellular spreading may be important for the
Ag-specific immune response and antitumor effect generated by VP22/E7
DNA. In this study, we tested a construct lacking the property of
intercellular spreading, VP221–267/E7 DNA, and
found that this vaccine was unable to generate significant enhancement
of E7-specific CD8+ T cell activity or protection
against TC-1 tumors. However, it is not clear whether the property of
intercellular spreading alone is sufficient to account for the
enhancement of the E7-specific CD8+ T cell immune
response. Our examination of certain molecules with trafficking
properties revealed that E7 linked with molecules derived from TAT,
MTS, or AH did not generate E7-specific CD8+ T
cell immune responses comparable to that generated by VP22/E7 DNA (Fig. 5). It is likely that the trafficking properties of these molecules are
not the same as those of VP22. It would be interesting to further
characterize the trafficking properties of these molecules and
correlate their ability to enhance immune responses.
Recently, two additional examples of proteins with purported
intercellular spreading properties have emerged, bovine herpesvirus
VP22 (BVP22) (29) and Marek’s disease virus VP22
(30, 31), both of which are VP22 homologues. Bovine
herpesvirus VP22 shares 
22% amino acid identity to human
herpesvirus VP22. A previous study found that BVP22 trafficking may be
more proficient than that of human VP22 after endogenous synthesis
(29). Marek’s disease virus VP22 shares 17% amino
acid identity to human herpesvirus VP22 (30) and may be
capable of intercellular transport after exogenous application
(31). Interestingly, mice vaccinated with DNA encoding
Marek’s disease virus VP22 linked to E7 exhibited enhanced E7-specific
CD8+ T cell activities compared with mice
vaccinated with wild-type E7 DNA (C.-F.H., unpublished observation). It
would be important to perform a head-to-head comparison of these
molecules in the future to identify the fusion construct that is most
potent at generating Ag-specific immunity and antitumor effects.
Recently, we also have tested the VP22 strategy in a naked Sindbis virus self-replicating RNA vector (26). We found that naked DNA and naked Sindbis virus RNA vector generated enhancement of E7-specific CD8+ T cell immune responses and an antitumor effect in vaccinated mice, although studies of the immune effector cells essential to the antitumor effect generated by these vectors revealed some interesting differences. Our studies demonstrated that CD8+ T cells were important for the antitumor effects generated by VP22/E7 vaccines with naked DNA or naked Sindbis virus RNA vector, whereas CD4+ T cells were not essential for the antitumor effect generated by either vaccine. Depletion of CD4+ T cells after treatment with naked VP22/E7 DNA or naked VP22/E7 Sindbis virus RNA did not lead to any significant loss in antitumor effect (26). In contrast, we found that NK cells were essential for the antitumor effect generated by the naked VP22/E7 Sindbis virus RNA vaccine but were not as important in the naked VP22/E7 DNA vaccine. Thus, different types of vaccine vectors encoding the same gene may activate different subsets of effector cells that are important to the antitumor effect.
The intercellular spreading strategy can potentially be used in conjunction with other strategies to further enhance vaccine potency. We previously have used Mycobacterium tuberculosis heat shock protein 70 (HSP70) linked to E7 to significantly enhance MHC class I presentation of E7 Ag to CD8+ T cells (9). Furthermore, we have developed an endosomal/lysosomal targeting strategy with E7 chimerically linked to a signal peptide (Sig) and the endosomal/lysosomal sorting signal (derived from lysosome associated membrane protein, LAMP-1) to significantly enhance MHC class II presentation of Ag to CD4+ T cells (10). Because these two approaches may act through a different mechanism than VP22/E7, the vaccine potency of HSP70 or Sig/E7/LAMP-1 vaccines may be further enhanced if the intercellular spreading strategy is also implemented in the vaccine design.
In summary, our findings illustrate the promise of enhancing vaccine potency by linkage of VP22 to Ag, allowing for increased intercellular spreading of Ag and enhanced Ag-specific CD8+ T cell activity leading to potent antitumor effects in vivo. This promising approach may facilitate future vaccine development for the control of cancers and infectious diseases.
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Acknowledgments |
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Footnotes |
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2 W.-F.C. and C.-F.H. contributed equally to this paper.
3 Address correspondence and reprint requests to Dr. T.-C. Wu, Department of Pathology, Johns Hopkins University School of Medicine, Ross Research Building, Room 659, 720 Rutland Avenue, Baltimore, MD 21205.
4 Abbreviations used in this paper: HPV, human papillomavirus; GFP, green fluorescent protein; LDH, lactacte dehydrogenase; DC, dendritic cell; TAT, HIV TAT protein; MTS, membrane-translocating sequence; AH, Antennapedia homeodomain; HSV, herpes simplex virus; LAMP, lysosome-associated membrane protein.
Received for publication November 13, 2000. Accepted for publication February 22, 2001.
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References |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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-transinducing factor (VP16) of HSV-1. Arch. Virol. 140:355.[Medline]
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S. Garg, A. E. Oran, H. Hon, and J. Jacob The Hybrid Cytomegalovirus Enhancer/Chicken {beta}-Actin Promoter along with Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances the Protective Efficacy of DNA Vaccines J. Immunol., July 1, 2004; 173(1): 550 - 558. [Abstract] [Full Text] [PDF]
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 K. Devaraj, M. L. Gillison, and T.-C. Wu DEVELOPMENT OF HPV VACCINES FOR HPV-ASSOCIATED HEAD AND NECK SQUAMOUS CELL CARCINOMA Critical Reviews in Oral Biology & Medicine, September 1, 2003; 14(5): 345 - 362. [Abstract] [Full Text] [PDF]
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C.-F. Hung, W.-F. Cheng, L. He, M. Ling, J. Juang, C.-T. Lin, and T-C. Wu Enhancing Major Histocompatibility Complex Class I Antigen Presentation by Targeting Antigen to Centrosomes Cancer Res., May 15, 2003; 63(10): 2393 - 2398. [Abstract] [Full Text] [PDF]
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C.-F. Hung, L. He, J. Juang, T.-J. Lin, M. Ling, and T.-C. Wu Improving DNA Vaccine Potency by Linking Marek's Disease Virus Type 1 VP22 to an Antigen J. Virol., February 22, 2002; 76(6): 2676 - 2682. [Abstract] [Full Text] [PDF]
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