Supplemental Data for :

Donnadieu et al. Differential Roles of Lck and Itk in T Cell Response to Antigen Recognition Revealed by Calcium Imaging and Electron Microscopy

These movies show the Ca²⁺ and morphological responses induced by APCs in T cells overexpressing kinase-defective forms of Lck and Itk. The level of [Ca²⁺]_i in T cells is displayed in color ranging from blue (50-100 nM) to red (600-800 nM).

Movie 1

Movie 1: Ag-induced morphological and Ca²⁺ responses in one wild-type T8.1 cell. Please note the small contact interface which occurs before the Ca²⁺ increase. Frame interval, 5 s.

Movie 2

Movie 2: High magnification of T8.1/Ag-pulsed APC interaction. Morphological changes are characterized by extended lamellipodia and intense membrane ruffling. Frame interval, 4 s.

Movie 3

Movie 3: Ca²⁺ and morphological responses of three Lck-KD cells interacting with Ag-pulsed APCs. Two of the three T cells do not respond. However, one T cell displays marked morphological and Ca²⁺ responses. Frame interval, 11 s.

Movie 4

Movie 4: Ca²⁺ and morphological responses of two Itk-KD cells interacting with Ag-pulsed APCs. One T cell displays a weak Ca²⁺ response despite extensive shape changes. Frame interval, 12 s.

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