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Department of Medicine, University of Wales College of Medicine, Cardiff, Wales, United Kingdom;
Immunology Unit, Glaxo-Wellcome Medicines Research Centre, Stevenage, Hertfordshire, United Kingdom; and
Department of Immunology, University Hospital Utrecht, Utrecht, The Netherlands
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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subunit of phosphatidylinositol 3-kinase and the
immunoreceptor tyrosine-based inhibitory motif-containing receptors,
leukocyte-associated Ig-like receptor-1 (LAIR-1) and programmed death-1
(PD-1), were identified. Coimmunoprecipitation studies demonstrated
that the exclusive phosphotyrosine-containing protein associated with
SHP-1 in Jurkat T cells under physiological conditions is LAIR-1.
Significantly, this interaction is constitutive and was detected only
in the membrane-enriched fraction of cell lysates. Ligand engagement of
the SH2 domains of SHP-1 is a prerequisite to activation of the enzyme,
and, consistent with an association with LAIR-1, SHP-1 was found to be
constitutively active in unstimulated Jurkat T cells. Importantly, a
constitutive interaction between LAIR-1 and SHP-1 was also detected in
human primary T cells. These results illustrate the sustained
recruitment and activation of SHP-1 at the plasma membrane of resting
human T cells by an inhibitory receptor. We propose that this mechanism
may exert a constitutive negative regulatory role upon T cell
signaling. |
Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Due to the high degree of sequence conservation between SHP-1 and SHP-2, it is likely that SHP-1 PTP activity is regulated in a similar manner to SHP-2 (6), minimally requiring the engagement of the amino-terminal SH2 domain by phosphotyrosine-containing ligands. A number of candidate ligands for the SH2 domains of SHP-1 within T cells have been proposed, including the CD3 invariant chains (7), p95Vav (8), ZAP-70 (9), and CD5 (7, 10). However, SHP-1 preferentially binds phosphorylated immunoreceptor tyrosine-based inhibitory motifs (ITIMs) defined by the minimal consensus sequence I/V/LXYXXL/V (11) found in the cytosolic domains of the inhibitory receptor superfamily of proteins (12). Following tyrosine phosphorylation, ITIM-containing receptors recruit SHP-1 and negatively regulate cell signaling in a number of hemopoietic cell types where they inhibit cell-mediated cytotoxicity, cytokine secretion, and proliferation. Therefore, it is likely that ITIM-containing receptors are also important for SHP-1 recruitment and activation in T cells. Indeed, a number of ITIM-containing receptors are expressed in the T cell lineage including two members of the Ig superfamily, PD-1 (13, 14) and leukocyte-associated Ig-like receptor-1 (LAIR-1) (15).
Studies in Jurkat T cells have highlighted the potential for SHP-1 to negatively regulate TCR signaling. The introduction into Jurkat T cells of a chimera consisting of the CD8 extracellular and transmembrane regions and the intracytoplasmic domain of the killer cell Ig-like receptor (KIR), NKB1, leads to a significant decrease in NF-AT/AP-1 reporter activity. This inhibitory effect is dependent on SHP-1 recruitment (16). In addition, the hyperresponsive TCR phenotype of Jurkat T cells over-expressing a catalytically inactive form of SHP-1 encoding intact SH2 domains has further demonstrated that SHP-1 is activated by a physiological ligand in Jurkat cells (9). However, the identity of the physiological ligand(s) responsible for activating SHP-1 in Jurkat T cells has not been established.
It has been proposed that tyrosine phosphorylated ZAP-70 (9) or CD5 (7, 10) may satisfy the requirement for SHP-1 SH2 domain engagement and activation in Jurkat T cells. The results presented here now suggest that constitutively, tyrosine-phosphorylated LAIR-1 may in fact be the physiological ligand that allows the recruitment and activation of SHP-1 in Jurkat T cells. In addition, LAIR-1 is found constitutively associated with SHP-1 in human primary T cells. Together, these results suggest that the complex of LAIR-1 and SHP-1 may contribute to a basal threshold for TCR signaling in human T cells.
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Yeast vector, pYTH9, and yeast strain, Ylck, were as described in Ref. 17 . Amino acids 1–222 of SHP-1 containing the two SH2 domains were cloned in frame into pYTH9, which contains amino acids 1–147 (the binding domain) of Gal4 protein to generate the bait construct pYTH9/BDSHP1SH2. Strain Ylck/BDSHP1SH2 was generated by integration of pYTH9/BDSHP1SH2 at the trp1 locus of Ylck. Transformants were cloned by restreaking for single clones on selective media. Recombinant yeast were maintained on selective media. Amino acids 728–864 of CD22 were cloned in-frame downstream of the activation domain of Gal4 in the pACT2 vector (Clontech Laboratories, Palo Alto, CA) to serve as a positive control.
All yeast manipulations were performed according to standard yeast
genetic protocols. Competent yeast cells were prepared and transformed
using the lithium acetate/Tris-EDTA/polyethylene glycol protocol
(17). The cDNA library was donated by M. Suda of
GlaxoNippon (Tsukuba, Japan). The cDNA was generated from
poly(A)+ RNA extracted from a mixture of
nonactivated and
12-O-tetradecanoylphorbol-13-acetate-activated H9 3D8 T
cells and synthesized using an oligo(dT) primer and random primers.
cDNA was unidirectionally cloned between the EcoRI and
XhoI sites of pACT2. cDNA library screening was performed
using Ylck/BDSHP1SH2. Approximately 1 x
1010 cells were transformed with 50 µg of H9
library DNA and plated onto minimal agar containing yeast nitrogen
base, 2% glucose, and 5 mM 3-amino-1,2,4-triazole, a competitive
inhibitor of the HIS3 gene, one of the reporter genes in this strain.
Transformation plates were incubated at 30°C for 10–14 days. Large
colonies were selected from these plates and streaked onto smaller
plates with or without methionine but supplemented with histidine.
These were left to grow for 3 days and then filter-lifted and screened
for protein-protein interactions using the LacZ reporter
(17). -Galactosidase activity was monitored using a
freeze-thaw fracture assay. Positive clones were deemed to be those
yeast that only turned blue on media lacking methionine. Library
plasmid DNA was recovered from yeast by enzymatic disruption of the
cell wall by treatment with Zymolyase-100T (ICN Biomedicals, Costa
Mesa, CA), alkaline lysis extraction, and amplification in
Max-Efficiency DH5-
Escherichia coli (Life Technologies,
Paisley, U.K.). Plasmids were re-introduced into the screening strain
or Ylck with the Gal4 binding domain alone to confirm specificity.
Sequence data was generated using ABI FS-Taq automated
sequencing protocols (PE Applied Biosystems, Foster City, CA). GST
SHP-1 plasmids R30K, R132K, and R30K/R132K (18) formed the
templates for PCR to generate SHP-1SH2 domain (amino acids 1–222)
encoding fragments with the appropriate mutations. These fragments were
cloned into the episomal yeast expression vector, pAS-CYH2
(19), transformed together with the individual pACT clones
into Ylck, and assayed as described above.
Cells
Human leukemic Jurkat T cell line E6.1 was obtained from the European Collection of Animal Cell Cultures (Salisbury, U.K.). The mouse IL-2-dependent T cell line CTLL-2 was kindly provided by Dr. Doreen Cantrell (Imperial Cancer Research Fund, London, U.K.). Jurkat and CTLL-2 T cells were maintained in RPMI 1640 supplemented with 10% FCS, 2 mM L-glutamine, 100 IU/ml penicillin, and 100 µg/ml streptomycin with the addition of 360 IU/ml rIL-2 (Chiron, Harefield, U.K.) for CTLL-2. Human blood was obtained from the blood transfusion service, and PBMCs were isolated by Ficoll-Hypaque density gradient centrifugation. T lymphoblasts were generated by stimulating PBMCs for 72 h at 37°C with 2 µg/ml PHA in complete RPMI 1640 medium supplemented with 360 IU/ml rIL-2. The cells were washed thoroughly and cultured for a further 48 h in complete RPMI 1640 medium supplemented with IL-2.
Abs and reagents
The mouse mAb, UCHT-1 (IgG1), used for activation of human T
cells, was raised to the extracellular domain of the human CD3
invariant chain (20) and was kindly provided by Dr. Doreen
Cantrell (Imperial Cancer Research Fund). Rabbit antiserum to the SH2
domains of SHP-1 was as previously described (1). The mAb
against LAIR-1 (DX26, IgG1) was as described (15). The
bi-specific F(ab')2, CD3 x CD28 (OKT3
x 9.3) Ab was kindly provided by Prof. Martin Glennie (Tenovus
Research Laboratory, University of Southampton, Southampton, U.K.).
Goat antiserum to CD3 and rabbit antiserum to p42/44
mitogen-activated protein kinase (MAPK) were purchased from Santa Cruz
Biotechnology (Santa Cruz, CA) and New England Biolabs (Beverly, MA),
respectively. The SHP-2 mAb was purchased from BD Transduction
Laboratories (Franklin Lakes, NJ), and the
p56lck and anti-phosphotyrosine (4G10) mAbs
were purchased from Upstate Biotechnology (Lake Placid, NY). GST fusion
proteins encoding the amino- and carboxyl-SH2 domains of SHP-1 were as
described in Ref. 1 . GST fusion proteins encoding the
R30K, R132K, and R30K/R132K mutations in the SH2 domains of SHP-1 were
kindly provided by Dr. Taolin Yi (Cleveland Clinic Foundation Research
Institute, Cleveland, OH) (18).
Subcellular fractionation
A total of 0.5–1.0 x 108 Jurkat T
cells were left unstimulated or stimulated with either 10 µg/ml
UCHT-1 for 3 min at 37°C or with 200 µM pervanadate (PV) for 10 min
at 37°C. Following stimulation, cells were pelleted and resuspended
in 2 ml ice-cold hypotonic lysis buffer (10 mM Tris-HCl (pH 7.5), 1 mM
MgCl2, 1 mM sodium orthovanadate) containing
protease inhibitors (10 µg/ml aprotinin, 10 µg/ml leupeptin, 10
µg/ml pepstatin, 1 mM EDTA, 1 mM PMSF) added immediately before use.
After 30 min incubation on ice, lysis was facilitated by Dounce
homogenization for 12 strokes on ice, and lysates were clarified to
remove nuclear material and intact cells. Supernatants were transferred
into ultracentrifugation tubes (Beckman Coulter, Fullerton, CA), and
fractionation was performed at 100,000 x g for 30 min
at 4°C (TL-100 Ultracentrifuge; Beckman Coulter). The supernatant
containing the soluble (S100) fraction was removed, and NaCl and
Nonidet P-40 were added to a final concentration of 150 mM and 0.5%,
respectively. The pellet constituting the particulate (P100) fraction
was solubilized in 1 ml Nonidet P-40 lysis buffer containing protease
inhibitors. Total cell lysates (
1–3 x
106 cell equivalents) were prepared for SDS-PAGE
by the addition of 6x Laemmli reducing buffer.
Detergent lysis
A total of 5.0 x 107 T lymphoblasts were left unstimulated or stimulated with either 10 µg/ml UCHT-1 for 3 min at 37°C or with 200 µM PV for 10 min at 37°C. Following stimulation, cells were centrifuged and lysed in 1 ml ice-cold 0.5% Nonidet P-40 lysis buffer (150 mM NaCl, 50 mM Tris-HCl (pH 8.0), 25 mM NaF, 1 mM sodium orthovanadate, 0.5% Nonidet P-40) containing protease inhibitors as above.
Immunoprecipitation and immunoblot analysis
Prepared cell lysates were incubated by continuous inversion for 1 h at 4°C with either purified monoclonal or polyclonal Abs. Immune complexes were trapped by rotating at 4°C for a further hour with the addition of protein A or protein G Sepharose slurry. GST fusion proteins immobilized on glutathione-agarose beads were similarly incubated with lysates for 90 min at a concentration of 20 µg/ml. Captured complexes were pelleted by centrifugation and washed in 0.5% Nonidet P-40 lysis buffer an additional two times. Following washes, beads were resuspended in 2x Laemmli reducing buffer or 2x Laemmli nonreducing buffer for samples to be immunoblotted with the anti-LAIR-1 mAb, DX26. Immunoprecipitations and cell lysates were resolved by 12% SDS-PAGE, transferred onto polyvinylidene difluoride membranes (Millipore, Bedford, MA), and probed with specific Abs followed by HRP-conjugated secondary Abs. Proteins were detected by ECL (Amersham Pharmacia Biotech, Piscataway, NJ). Blots were stripped for 30 min at 55°C (100 mM 2-ME, 2% SDS, 62.5 mM Tris-HCl (pH 6.8)) before reprobing.
PTP activity assay
SHP-1 was immunoprecipitated from the particulate (P100) and cytosolic (S100) fractions of unstimulated or CD3-stimulated Jurkat T cells as described above. Immunoprecipitates were assayed for PTP activity using 32P-labeled Raytide as described in Ref. 1 . PTP activities in the immunoprecipitates were normalized by performing densitometry on the amount of SHP-1 detected in parallel anti-SHP-1 immunoprecipitations. Normalizations were further confirmed by performing serial dilutions of the SHP-1 immunoprecipitations from the S100 fraction before anti-SHP-1 immunoblotting.
Analysis of detergent-resistant membrane domains
A total of 1 x 108 Jurkat T cells were left untreated or treated with 0.2% saponin for 10 min at 4°C and lysed on ice for 30 min in 1 ml 1% Triton X-100 in MNE buffer (25 mm MES (pH 6.5), 150 mM NaCl, 5 mM EDTA-containing protease inhibitors). The lysates were Dounce homogenized as above, mixed with 1 ml 80% sucrose in MNE buffer, and transferred to an ultracentrifuge tube. The sample was overlaid sequentially with 2 ml 30% and 1 ml 5% sucrose in MNE buffer and centrifuged for 18 h at 4°C at 200,000 x g. Then, 0.4-ml fractions were collected from the top of the gradient and solubilized in 6x Laemmli nonreducing buffer.
IL-2 release assay
Jurkat T cells were stimulated in triplicate on 96-well flat-bottom plates at 2 x 105 cells/well with a titration of bi-specific F(ab')2 CD3 x CD28 Ab in the presence of LAIR-1 (DX26) or control IgG1Ab (5 µg/ml) cross-linked with a rabbit anti-mouse Ab (10 µg/ml). After 6 h, the supernatants were harvested. For a measure of IL-2 production, extensively washed CTLL-2 cells were added to diluted supernatants (1:2) and incubated for 40 h with [3H]thymidine (1 µCi/well) present for the last 16 h of culture before harvesting, and the incorporated radioactivity was assessed.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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The identities of phosphotyrosine-containing proteins capable of
interacting with the SH2 domains of SHP-1 in T cells have not been
readily forthcoming. Therefore, we modified a known cDNA cloning
strategy, the yeast two-hybrid, to create a yeast tri-hybrid
(17) to identify, from T cells, those phosphotyrosine
proteins capable of interacting with the SH2 domains of SHP-1. A stable
yeast strain, Ylck/BDSHP1SH2, was generated expressing both the active
form of the protein tyrosine kinase lck under a regulatable promoter
and a chimeric cDNA encoding the tandem SH2 domains (amino acids
1–222) (1) of SHP-1 fused to the DNA binding domain of
Gal4. The regulatable expression of lck in the recombinant yeast via
the MET3 promoter allowed for the identification of interactions that
were dependent on the tyrosine phosphorylation of either bait or prey
proteins (17). The validity of the yeast tri-hybrid system
for identifying ligands of the SH2 domains of SHP-1 was established
using CD22 as a positive control (results not shown). Ylck/BDSHP1SH2
was transformed with a cDNA library derived from the H9 T cell line.
The cDNAs from positive clones were isolated and sequenced, and three
proteins were identified (Fig. 1
). They
were PD-1 (one clone encoding amino acids 226–289 and encompassing
part of the intracellular domain and only one ITIM of the two possible
ITIMs contained in this protein), LAIR-1 (one clone encoding amino
acids 56–288 and encompassing most of the extracellular domain, the
transmembrane domain, and all of the intracellular region including
both ITIMs of this protein), and the -type 85-kDa subunit (p85)
of phosphatidylinositol 3-kinase (two clones encoding amino acids
64–729 and 321–729, with no consensus ITIM motifs contained within
these proteins).
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). In addition, as demonstrated in
yeast strain Ylck/BDSHP1SH2R30K, an intact amino-terminal SH2 domain of
SHP-1 appeared to be critically required for the physical association
of the SHP-1 (amino acids 1–222) chimera with LAIR-1 and p85 but
not PD-1 (Fig. 1). Catalytically active SHP-1 is constitutively associated with the membrane fraction of Jurkat T cells
Having demonstrated that the SH2 domains of SHP-1 could interact
with the intracytoplasmic domains of LAIR-1 and PD-1 and with p85 in
yeast, we were interested in establishing whether any of these
interactions could be detected in T cells. First, LAIR-1 and PD-1 are
transmembrane receptors, and we reasoned that any physiological
associations with SHP-1 would be occurring predominantly at the cell
membrane. Therefore, Jurkat T cells were subjected to subcellular
fractionation to generate membrane (P100) and cytosolic (S100)
fractions. The validity of the fractionation procedure was evaluated by
immunoblotting with anti-CD3 and MAPK Abs (Fig. 2A). As previously reported
for other leukocytes, over 90% of SHP-1 was localized to the cytosolic
fraction (22). However, 5–10% of SHP-1 was found
constitutively associated with the P100 fraction in Jurkat T cells
(Fig. 2A). The amount of SHP-1 in the P100 fraction did not
significantly alter upon anti-CD3 or PV treatment (Fig. 2A). To examine the catalytic activity of SHP-1 in the P100
fraction of Jurkat T cells, SHP-1 was immunoprecipitated from the P100
and S100 fractions of unstimulated or anti-CD3-stimulated Jurkat T
cells, and the PTP activity was measured (Fig. 2B). The
catalytic activity of SHP-1 in the P100 fraction was found to be 2- to
3-fold higher than that of SHP-1 in the S100 fraction. It is believed
that the PTP activity of SHP-1 is liberated through the occupation of
the amino-terminal SH2 domain by phosphotyrosine-containing ligands.
Therefore, we sought to identify the phosphotyrosine-containing
protein(s) responsible for activating SHP-1 within the membrane
fraction.
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SHP-1 was immunoprecipitated from the P100 and S100 fractions of
unstimulated anti-CD3 or PV-stimulated Jurkat T cells, and the
immunoprecipitates were analyzed by anti-phosphotyrosine
immunoblotting (Fig. 3). Within the P100
fractions, SHP-1 only coimmunoprecipitated a 42-kDa
phosphotyrosine-containing protein (pp42). The 26-kDa
phosphotyrosine-containing protein found in the P100 fraction likely
represents CD3 that is immunoprecipitated by the stimulating Ab
because it was detected in both control and anti-SHP-1
immunoprecipitations following anti-CD3 activation. Furthermore,
the preclearing of the lysates resulted in the disappearance of the
26-kDa phosphotyrosine-containing protein (results not shown). No
phosphotyrosine proteins were found to coimmunoprecipitate with SHP-1
in the S100 fraction, although SHP-1 itself was constitutively tyrosine
phosphorylated in this fraction (Fig. 3, and results not shown).
Significantly, SHP-1 was constitutively associated with pp42 in the
P100 fraction of unstimulated Jurkat T cells (Fig. 3). However,
anti-CD3 stimulation resulted in an apparent decrease in the
tyrosine phosphorylation of pp42 associated with SHP-1 in the P100
fraction. Parallel immunoprecipitations with SHP-1 and SHP-2 antisera
from the P100 fraction demonstrated the constitutive association of
pp42 with SHP-1 but not SHP-2 (Fig. 4A). The SH2 domains of SHP-1
mediated the association of pp42 with SHP-1. Lysates from the
particulate fraction of unstimulated Jurkat T cells were absorbed onto
GST or a GST fusion protein encoding the tandem SH2 domains of SHP-1.
The tandem SH2 domains of SHP-1 (Fig. 4B) specifically
absorbed pp42.
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The SDS-PAGE migration properties of pp42 associating with SHP-1
in Jurkat T cells resembled that of the ITIM-containing receptor,
LAIR-1. The identity of pp42 as LAIR-1 was confirmed by immunoblotting
SHP-1 immunoprecipitations from P100 fractions with LAIR-1 antisera
(Fig. 5A). Immunoprecipitation
of LAIR-1 followed by anti-phosphotyrosine immunoblotting confirmed
that LAIR-1 was constitutively tyrosine phosphorylated in the membrane
fraction of unstimulated Jurkat T cells (Fig. 5B). In
contrast to the decrease in the tyrosine phosphorylation of pp42
associated with SHP-1 (Fig. 3), anti-CD3 treatment of Jurkat T
cells resulted in no change in the amount of LAIR-1 associated with
SHP-1 (Fig. 5A). In addition, anti-CD3 stimulation had
no effect on the basal level of tyrosine phosphorylation of LAIR-1
(Fig. 5B). In parallel experiments, stimulation of Jurkat T
cells with soluble anti-CD3 (UCHT-1) could be demonstrated to
result in an extensive tyrosine phosphorylation of the CD3 chains and
downstream signaling mediators (results not shown).
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To understand the mechanism of how LAIR-1 is constitutively
tyrosine phosphorylated, we hypothesized that LAIR-1 could be localized
in a specialized subcompartment in the membrane that allowed it access
to an active protein tyrosine kinase under basal conditions. One method
for separation of membrane subcompartments based on their
glycosphingolipid and cholesterol content is sucrose density gradient
fractionation following lysis of cells in Triton X-100 at 4°C
(23). Saponin treatment can be used to identify
cholesterol-enriched membrane fractions by depleting cholesterol and
rendering them soluble in Triton X-100 (24). Jurkat T
cells were left untreated or treated with saponin, extracted with
Triton X-100, and fractionated on a sucrose gradient (Fig. 6). Among the 12 fractions collected,
fractions 2 and 3 contained the classic low-sucrose-density
detergent-insoluble glycosphingolipid-enriched domain
(25), as demonstrated by the localization of
p56lck in these fractions (26).
Fractions 8–12 from the gradient represented the Triton X-100
soluble fractions. Interestingly, LAIR-1 was found excluded from
the low-sucrose-density,detergent-insoluble
glycosphingolipid-enriched domain-containing membrane
compartment, but was found localized in part to a saponin-sensitive,
intermediate-sucrose-density membrane compartment (fraction 7) (Fig. 6). As a negative control, CD45 localized to the high-sucrose-density
membrane compartment (27) that remained insensitive to
saponin treatment.
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LAIR-1 possesses a single Ig-like domain in its extracellular
region (15), suggesting the existence of a cell surface
ligand that would potentially regulate the localization of LAIR-1 in
the membrane. A ligand for LAIR-1 could potentially either cluster
LAIR-1 away from the TCR or recruit LAIR-1 to the TCR, thereby lowering
or raising the threshold for TCR signaling, respectively. In addition,
a ligand could regulate the tyrosine phosphorylation of LAIR-1 and
consequently determine SHP-1 recruitment to the cell membrane. In the
absence of an identified ligand for LAIR-1, we used the anti-LAIR-1
mAb, DX26 (15), to cross-link LAIR-1, in conjunction with
a soluble bi-specific Ab directed against CD3 and CD28, and examined
the effects on IL-2 production. No significant differences in IL-2
production in response to CD3 and CD28 cross-linking were detected as a
result of the simultaneous cross-linking of LAIR-1 on Jurkat T cells
(Fig. 7). The direct co-cross-linking of
LAIR-1 and CD3 also had no effect on IL-2 production in Jurkat T cells
(results not shown).
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Having detected a constitutive interaction between SHP-1 and
LAIR-1 in human transformed T cells, we investigated whether a similar
association occurred in human primary T cells. In this regard, LAIR-1
was immunoprecipitated from T lymphoblasts with the anti-LAIR-1
mAb, DX26 (15), and was demonstrated to be constitutively
associated with SHP-1 (Fig. 8). Anti-CD3
stimulation resulted in a detectable increase in the amount of SHP-1
found associated with LAIR-1 (Fig. 8). No SHP-1 could be detected
coimmunoprecipitating with an isotype-matched control for DX26 (result
not shown). In contrast to Jurkat T cells, the basal and
anti-CD3-stimulated level of tyrosine phosphorylation of LAIR-1 was
below a level of detection in the T lymphoblasts, and only upon PV
treatment did LAIR-1 tyrosine phosphorylation become apparent. As with
Jurkat T cells, anti-LAIR-1 cross-linking with the mAb DX26 on T
lymphoblasts had no effect on the basal LAIR-1/SHP-1 association
(results not shown).
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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does not possess any
ITIMs, and so the molecular basis of the interaction between p85 and
the SH2 domains of SHP-1 in the yeast requires further analysis. Having demonstrated that PD-1, LAIR-1, and p85 can interact with the SH2 domains of SHP-1 in yeast, it was important to demonstrate physiological interactions in T cells. We initially focused on the transformed T cell line Jurkat because functional data implicating SHP-1 in regulating thresholds of TCR activation have been obtained using this line (9). However, we were unable to detect significant coimmunoprecipitation between SHP-1 and any phosphotyrosine-containing proteins in unstimulated or anti-CD3-stimulated Jurkat cell detergent lysates. Therefore, although phosphotyrosine-containing ligand(s) of SHP-1 must exist in Jurkat T cells in lieu of previously described functional data (9), these ligand-SHP-1 complexes must occur at very low levels.
We detected no interaction between tyrosine-phosphorylated p85 or
and SHP-1 in Jurkat T cells under any conditions. Therefore, the
physiological significance of the interaction of the SH2 domains of
SHP-1 and tyrosine-phosphorylated p85 in the yeast remains to be
determined. Likewise, we were unable to detect an interaction of SHP-1
and a tyrosine-phosphorylated molecule of the approximate molecular
mass of PD-1 (31 kDa) in Jurkat T cells (29). For this
reason, PD-1 is unlikely to be the ligand implicated in the activation
of SHP-1 in Jurkat T cells. Instead, the major physiological
phosphotyrosine-containing ligand of SHP-1 in Jurkat T cells is
identified as LAIR-1. Significantly, this interaction was demonstrated
to be constitutive, and therefore it is likely that the constitutive
LAIR-1 interaction with SHP-1 accounts for the hyperresponsiveness of
TCR signaling following the introduction of catalytically inactive
SHP-1 into Jurkat T cells (9). Importantly, the
constitutive interaction of LAIR-1 and SHP-1 is not an exclusive
feature of Jurkat T cells, but can also be detected in primary human T
cells.
The effects of anti-CD3 stimulation on the interaction of LAIR-1 and SHP-1 in Jurkat T cells and T lymphoblasts were distinct. In Jurkat T cells, the triggering of CD3 led to no increases in the basal association of LAIR-1 and SHP-1. Paradoxically, the tyrosine phosphorylation of LAIR-1 that specifically coimmunoprecipitated with SHP-1 was reproducibly decreased in multiple experiments following anti-CD3-mediated activation. This decrease in phosphorylation of LAIR-1 appears to be limited to that bound to SHP-1 because no detectable change in total LAIR-1 phosphorylation occurs. It is possible that anti-CD3 stimulation induces a dephosphorylation of one of the ITIMs of LAIR-1 that is associated with SHP-1. However, it is conceivable that although both ITIM motifs in LAIR-1 may be necessary for the initial recruitment of SHP-1, once SHP-1 has bound LAIR-1, the dephosphorylation of one of the ITIMs on LAIR-1 may not cause a significant loss of SHP-1 binding. In contrast, the triggering of CD3 on T lymphoblasts results in a small increase in the amount of SHP-1 associated with LAIR-1, despite our inability to detect increases in the phosphotyrosine content of LAIR-1. We assume that the basal difference in the intensity of LAIR-1 tyrosine phosphorylation in Jurkat vs primary T cells is a consequence of a higher protein tyrosine kinase activity in the transformed T cells. Nevertheless, the key phenomenon of a constitutive association between LAIR-1 and SHP-1 is conserved in both primary and transformed T cells.
The constitutive tyrosine phosphorylation of LAIR-1 in human T cells illustrates a recurrent feature of ITIM-containing receptors. Both CD22 (30) on primary splenic B cells, and paired Ig-like receptor B on bone marrow-derived macrophages (31, 32) and primary splenic B cells (33) have been demonstrated to be constitutively tyrosine phosphorylated. A low level of constitutive tyrosine phosphorylation would enable ITIM-containing receptors to contribute to the establishment of basal thresholds of activation. The examination of thymocytes and peripheral T cells from SHP-1-deficient mice has provided substantial evidence to implicate SHP-1 in regulating the TCR signaling thresholds that govern T cell development and the activation of mature T cells (3, 4, 5, 7, 34). However, the molecular basis for SHP-1 activation in mouse thymocytes and peripheral T cells still remains to be fully elucidated. With regard to the constitutive activation of SHP-1 human T cells, it is particularly intriguing that the phenotype of motheaten lymph node T cells expressing the transgenic TCR, F5, suggests that SHP-1 is constitutively active in resting primary lymph node T cells (4). Thus far, there have been no reports of a mouse homologue of LAIR-1, and hence it is possible that one or more additional members of the family of ITIM-containing receptors are responsible for SHP-1 activation in mouse thymocytes and peripheral T cells.
Src-like kinases have been implicated in the PV-mediated phosphorylation of LAIR-1 in Jurkat T cells (35) and may presumably mediate the basal tyrosine phosphorylation of LAIR-1. Interestingly, p56lck isolated from low-sucrose-density membrane fractions has been demonstrated to possess low kinase activity (27), although full TCR signaling function nevertheless requires the S-acylation of p56lck (36). Therefore, it is conceivable that active p56lck may be in a region of the membrane corresponding to the saponin-sensitive, intermediate-sucrose-density membrane fraction. Furthermore, the localization of a subpool of LAIR-1 to the intermediate-sucrose-density membrane compartment in unstimulated Jurkat may thereby expose it to active p56lck.
The basal phosphorylation of LAIR-1 leading to constitutive activation of SHP-1 suggests that SHP-1 may dephosphorylate the TCR complex or a downstream component of the TCR signaling pathway, thereby contributing to the generation of a basal threshold of activation of T cells. However, it is not apparent how a LAIR-1-SHP-1 complex would gain access to the TCR or TCR signaling pathway. Nevertheless, it would be predicted from the results with catalytically inactive SHP-1 (9) that a LAIR-1-SHP-1 complex is able to influence the outcome of TCR signaling without a requirement for specific coengagement of LAIR-1 and TCR. Similarly, the introduction of catalytically inactive SHP-1 by transfection into mouse T cell hybridoma lines (3, 9) or by transgenesis into primary thymocytes (5) results in an increased sensitivity to anti-CD3 stimulation, without a requirement for the coengagement of any additional receptors. In addition, expression of a HLA-A2/SHP-1 chimera in Jurkat T cells requires no specific coligation with the TCR to be able to negatively affect anti-CD3-triggered signaling (37). Finally, the MHC class I engagement of a KIR on a CD4+ T cell clone expressing a MHC class II-restricted TCR is sufficient to regulate cytokine production elicited by bacterial superantigen (38). Taken together, these results suggest that the recruitment of SHP-1 to the plasma membrane, without a direct physical juxtaposition to the TCR, may be sufficient to influence the outcome of TCR signaling.
LAIR-1 possesses a single Ig-like domain in its extracellular region, suggesting the possible existence of a cell surface ligand. In an attempt to mimic the potential effects of LAIR-1 ligand engagement, independent cross-linking of LAIR-1 or simultaneous co-cross-linking with CD3 on Jurkat T cells was performed. Under both conditions, anti-CD3-induced production of IL-2 was unaffected. Furthermore, in keeping with these functional observations, the direct cross-linking of LAIR-1 with DX26 on Jurkat or primary T cells resulted in no increase in either the phosphotyrosine content of LAIR-1 or SHP-1 recruitment. However, the recent demonstration that treatment of Jurkat T cells with a polyclonal serum directed against LAIR-1 results in a large increase in the phosphotyrosine content of LAIR-1 (35) still leaves open the possibility that the physiological ligand of LAIR-1 may perform a similar function in vivo. The direct engagement of LAIR-1 by its ligand would potentially lead to increased tyrosine phosphorylation of the receptor and SHP-1 recruitment and a subsequent further raising of the TCR-signaling threshold. We believe the discrepancies in the ability of the different anti-LAIR-1 Abs to induce the tyrosine phosphorylation of LAIR-1 may be attributed to the particular epitopes recognized by these Abs.
Our finding of a constitutive interaction between LAIR-1 and SHP-1 in human T cells provides an opportunity to evaluate the importance of LAIR-1 to SHP-1 activation in terms of the capacity of SHP-1 to regulate thresholds of TCR signaling. Furthermore, it will stimulate a dissection of the molecular requirements for LAIR-1 tyrosine phosphorylation and inhibitory function in human T cells.
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2 Present address: Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, MO 63110.
3 Address correspondence and reprint requests to Dr. R. James Matthews, Department of Medicine, Tenovus Building, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XX, Wales, U.K.
4 Abbreviations used in this paper: SH2, Src homology 2; SHP, SH2 domain-containing protein tyrosine phosphatase; PTP, protein tyrosine phosphatase; LAIR-1, leukocyte-associated Ig-like receptor-1; ITIM, immunoreceptor tyrosine-based inhibitory motif; KIR, killer cell Ig-like receptor; MAPK, mitogen-activated protein kinase; PV, pervanadate.
Received for publication June 22, 2000. Accepted for publication November 9, 2000.
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  L. Meyaard The inhibitory collagen receptor LAIR-1 (CD305) J. Leukoc. Biol., April 1, 2008; 83(4): 799 - 803. [Abstract] [Full Text] [PDF]
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 |
|
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|
|
|
|
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|
|
|
|
 |
|
 A. Verbrugge, T. d. Ruiter, H. Clevers, and L. Meyaard Differential contribution of the immunoreceptor tyrosine-based inhibitory motifs of human leukocyte-associated Ig-like receptor-1 to inhibitory function and phosphatase recruitment Int. Immunol., November 1, 2003; 15(11): 1349 - 1358. [Abstract] [Full Text] [PDF]
|
|
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|
 |
|
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I. Miller, G. Hatzivassiliou, G. Cattoretti, C. Mendelsohn, and R. Dalla-Favera IRTAs: a new family of immunoglobulinlike receptors differentially expressed in B cells Blood, April 15, 2002; 99(8): 2662 - 2669. [Abstract] [Full Text] [PDF]
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