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The Journal of Immunology, 2000, 164: 3950-3954.
Copyright © 2000 by The American Association of Immunologists

CUTTING EDGE

Cutting Edge: Increased Expression of Bcl-2 in Antigen-Specific Memory CD8+ T Cells1

Jason M. Grayson, Allan J. Zajac2, John D. Altman and Rafi Ahmed3

Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Bcl-2 plays a critical role in regulating cell survival and apoptosis. We examined Bcl-2 expression in virus-specific CD8 T cells during the expansion, death, and memory phases of the T cell response following infection of mice with lymphocytic choriomeningitis virus (LCMV). Naive CD8 T cells expressed a basal level of Bcl-2 that was down-regulated in effector CD8 T cells just before the death phase. Bcl-2 levels remained low during the death phase but surviving memory CD8 T cells expressed higher levels of Bcl-2 than naive cells. These changes were shown to occur in LCMV TCR transgenic cells as well as virus-specific CD8 T cells in C57BL/6 and BALB/c mice identified by MHC class I tetramers. In all instances, memory CD8 T cells expressed higher levels of Bcl-2, suggesting that increased Bcl-2 expression plays a role in the long-term maintenance of memory CD8 T cells in vivo.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
It is now well established that memory T cells can persist for extended periods in vivo (1, 2) The pool of memory T cells can remain remarkably stable and this constancy in numbers is maintained by a balance between cell survival, apoptosis, and proliferation (3, 4, 5). The mechanisms that regulate the life span of memory T cells are not understood at the molecular level. We have begun to address this question by examining expression of molecules that play a role in cell survival and apoptosis.

One of the most widely studied regulators of cell death is the Bcl-2 superfamily. This family is composed of more than 15 family members in humans, mice, and worms. Some members of the family are proapoptotic (Bad, Bax, Bid) while others (Bcl-2 and Bcl-xL) exhibit anti-apoptotic functions (6). At any given time, a cell’s susceptibility to apoptosis is determined by a ratio of the pro- to anti-apoptotic factors. The prototypic member of this gene family is Bcl-2. Originally identified as a translocation in B cell lymphoma (7), overexpression of Bcl-2 provides protection against cell death by preventing release of cytochrome c after mitochondrial damage (8).

Several studies have shown that Bcl-2 plays a role in T cell survival. The most compelling evidence comes from the observation that Bcl-2-/- mice exhibit rapid loss of peripheral T cells (9, 10). Also, it has been shown that activated T cells that exhibit increased susceptibility to apoptosis in vitro express lower levels of Bcl-2 (11). However, very little is known about Bcl-2 expression in Ag-specific T cells in vivo. In this study, we have examined the expression of Bcl-2 in CD8 T cells as they progress through the various stages of the immune response (naive to effector to memory) following infection of mice with lymphocytic choriomeningitis virus (LCMV).4 Using a transgenic T cell system and MHC class I tetramers to identify virus-specific CD8 T cells in normal mice, we show that memory CD8 T cells express higher levels of Bcl-2 than naive T cells. This finding suggests that Bcl-2 may play a role in the long-term survival of memory CD8 T cells.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Virus infection and mice

Six- to 8-wk-old female BALB/c or C57BL/6 mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Mice were infected with 2 x 105 PFU of LCMV Armstrong strain i.p. and used at the indicated time points. Virus stocks were grown and quantitated as described previously (12).

Priming of transgenic cells

Priming of P14 transgenic cells has been described before (13). The LCMV TCR P14 transgenic mice (B6 x 129) were obtained from The Jackson Laboratory and then backcrossed to C57BL/6 at Emory University. The transgenic mice used in this study were backcrossed six to eight times. Briefly 2 x 106 naive P14 splenocytes were injected i.v. into naive C57BL/6 hosts. Four hours after transfer, mice were infected with 2 x 105 PFU of LCMV Armstrong i.p. and used at the indicated time points.

Flow cytometry and FACS analysis

Preparation of cells and staining has been described previously (1).

Intracellular staining for Bcl-2

Cells (2 x 106) were surface stained as described above. After washing unbound Ab and MHC class I tetramer, the cells were subjected to intracellular staining for Bcl-2 using the CytoFix/Cytoperm kit according to the manufacturer’s instructions (PharMingen, San Diego, CA). For intracellular Bcl-2 staining, FITC-conjugated hamster anti-mouse Bcl-2 Ab (clone 3F11) or its isotype control Ab (hamster IgG) (PharMingen) was used.

Preparation of H-2Db and H-2Ld tetramers

The construction and purification of LdNP118-126, DbGP33-41, DbNP396-404, and DbGP276-86 MHC class I tetramers have been described previously (1).


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
To determine the level of Bcl-2 expression, we performed flow cytometric analysis using P14 transgenic CD8+ T cells. These CD8 T cells express a transgenic TCR specific for the LCMV epitope GP33-41 and allow a comparison of Ag-specific naive, effector, and memory CD8+ T cells. Fig. 1Go shows that naive Ag-specific CD8+ T cells had moderate levels of Bcl-2 that decreased in effector cells isolated 8 days postinfection. In addition to decreased levels of Bcl-2, effector cells contained decreased CD8 expression. The decreases in these molecules do not represent a global decrease in staining as effector cells express increased levels of CD44, CD11a, and CD43 (1B11) and intracellular IFN-{gamma} (data not shown). Memory cells (79 days postinfection) contained higher levels of Bcl-2 than those found in naive or effector cells.



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  		FIGURE 1. Bcl-2 expression in transgenic CD8+ T cells during an immune response. A total of 2 x 106 splenocytes was transferred from naive P14 transgenic mice into nonirradiated naive C57BL/6 hosts. Four hours after transfer, mice were infected with LCMV-Armstrong and used at the indicated time points. Spleen cells were double stained with anti-CD8{alpha} and DbGP33-41. The Bcl-2 levels of the gated populations are plotted in histogram format, with the mean fluorescence intensity indicated by the value in the upper right-hand corner of the plot. The Bcl-2 levels of naive CD8+ T cells are overlaid on each histogram as the dotted line.

 
In addition to examining Bcl-2 expression in a monoclonal response, we examined it during the CD8 response in BALB/c mice at various times postinfection. Fig. 2GoA shows that naive CD8+, LFA-1low T cells expressed a basal level of Bcl-2. Following infection with LCMV, Bcl-2 expression was essentially unchanged in Ag-specific cells at 5 days postinfection. However, at the peak of the response on day 8 postinfection (i.e., peak before the death phase), Bcl-2 levels were clearly lower in the Ag-specific population. These changes were observed in four independent experiments. Bcl-2 levels remained lower in virus-specific CD8 T cells during the death phase (days 8–20). The day 13 time point is shown in Fig. 2GoA. Memory cells that survived this death phase expressed higher levels of Bcl-2. Similar increased levels of Bcl-2 protein were observed in Ag-specific memory CD8 T cells isolated from pooled lymph nodes and PBLs (Fig. 2GoB).



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  		FIGURE 2. Bcl-2 levels in Ag-specific CD8 T cells identified by MHC class I tetramers. A, Spleen cells from naive and LCMV-infected (days 5, 8, 13, and 70 post infection) mice were double stained with anti-CD8{alpha} and either anti-CD11a/LFA-1 (naive) or MHC class I tetramer LdNP118-126 (infected). B, PBL and lymph node cells from immune mice (120 days postinfection) were incubated with anti-CD8{alpha} and LdNP118-126. The Bcl-2 levels of the gated populations are plotted in histogram format, with the mean fluorescence intensity indicated by the value in the upper right-hand corner of the plot. The Bcl-2 levels of naive CD8+ T cells are overlaid on each histogram as the dotted line.

 
In BALB/c mice, the majority of the antiviral CD8 T cell response is specific for one immunodominant epitope (NP118-126). We also tracked how Bcl-2 expression changes in C57BL/6 mice that make sizable CD8 T cell responses to three different LCMV epitopes. Ag-specific CD8 T cells were examined (Fig. 3Go) on day 8 (effector) and day 186 (memory) postinfection with LCMV. Using MHC class I tetramers, we examined Bcl-2 levels in cells specific for the Db-restricted epitopes GP33-41, NP396-404, and GP276-86. Ag-specific effector cells for all three epitopes showed remarkably similar decreases in Bcl-2 expression compared with naive CD8+CD44low cells. More important, memory cells for all three epitopes contained higher levels of Bcl-2 compared with naive CD8 T cells. Our observations of cells with different epitope specificities demonstrate that high Bcl-2 levels are a generalized feature of CD8 memory cells.



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  		FIGURE 3. Analysis of Bcl-2 expression among CD8 T cells of different Ag specificities identified by MHC class I tetramers. Spleen cells were isolated from C57BL/6 mice at days 8 and 186 postinfection with LCMV. Cells were stained with anti-CD8{alpha} and either DbGP33-41, DbNP396-404, or DbGP276-86 tetramer. The Bcl-2 levels of the gated populations are plotted in histogram format, with the mean fluorescence intensity indicated by the value in the upper right-hand corner of the plot. Additionally, the Bcl-2 levels of naive (CD8+CD44low) T cells are overlaid on each histogram as the dotted line.

 
Since Ag-specific memory CD8+ T cells contained higher levels of Bcl-2 than naive cells, this prompted us to examine Bcl-2 levels in total CD44high memory phenotype cells. Fig. 4Go confirms that memory phenotype (CD8+CD44high) CD8 cells of undefined specificity contain higher levels of Bcl-2 than naive phenotype cells (CD8+CD44low). Interestingly, this was not a characteristic of CD4 T cells and CD4+CD44high cells exhibited no difference in their Bcl-2 levels when compared with CD4+CD44low cells.



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  		FIGURE 4. Bcl-2 is expressed at high levels in memory phenotype CD8+, but not CD4+ T cells. Spleen cells from naive C57BL/6 stained with anti-CD44 and anti-CD8{alpha} or anti-CD4. The Bcl-2 levels of the gated populations are plotted in histogram format.

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
In this study, we tracked expression of Bcl-2 through all phases of a CD8 T cell response: activation/expansion, contraction, and memory phases. We found that naive CD8 T cells express a basal level of Bcl-2 that does not decrease in early effector cells (5 days postinfection) during the expansion phase. However, Ag-specific cells at 8 days postinfection (at the peak of the expansion phase and at the beginning of the death phase) express lower levels of Bcl-2. Throughout the death phase Bcl-2 remains low in Ag-specific CD8 T cells. Surviving memory cells contain higher levels of Bcl-2 than naive cells. These findings were observed in both monoclonal and polyclonal CD8 responses. Additionally, they were observed in multiple LCMV epitopes in both BALB/c and C57BL/6 mice.

What are the implications of higher Bcl-2 expression in CD8 memory T cells? To understand what Bcl-2 may be doing, it is critical to note some differences between memory and naive cells. Memory cells survive for extended periods in vivo and exhibit a higher rate of homeostatic proliferation than naive cells (3). Little is known about the rate of progression through the phases of the cell cycle in these cells. Previous studies of Bcl-2 overexpression have shown that higher levels of Bcl-2 correlate with an extended G1 phase (14). Additionally, Ag-induced cell death in vitro has been demonstrated to occur from a late G1 phase cell cycle checkpoint that can be blocked by expression of p16INK4a or by inactivation of the retinoblastoma tumor suppressor protein (pRb) (15). The Bcl-2 levels in memory cells could increase G1 phase, allowing the cell to repair damage accrued over its long life span and then to rapidly progress through the rest of the cycle. The higher rate of homeostatic proliferation observed in memory cells may simply be a consequence of increased survival of the G1 phase transition. The increased levels of Bcl-2 found in memory cells may also influence survival of these cells after they encounter Ag upon reinfection. The higher levels of Bcl-2 may lead to a diminished death phase after secondary infection, resulting in a net increase of memory cells.

Our studies confirm and extend previous findings of Bcl-2 expression in activated T cells in vitro and in vivo. Activation of T cells in vitro with strong mitogenic stimuli and IL-2 results in slightly increased levels of Bcl-2 (11, 16, 17). In LCMV infection, we only observe decreases in Bcl-2 after prolonged expansion and proliferation. Ag-specific cells 5 days postinfection contain basal levels of Bcl-2 but with further proliferation contain low levels by 8 days postinfection. This maintenance of basal levels of Bcl-2 after early TCR activation makes teleological sense. If Bcl-2 decreased immediately after TCR activation, then cells would be deleted and a chronic infection would ensue. Additionally, our findings extend observations made in CD8 T cells from EBV and HIV patients (18, 19). CD8 T cells from patients generally had Bcl-2 levels lower than those found in uninfected controls. In these studies, the cells are of undefined specificity but they exhibit lower levels of Bcl-2 similar to Ag-specific cells at the peak of LCMV infection.

We also addressed differences between naive and memory phenotype cells of undefined specificity for both CD8 and CD4 cells. CD8 cells of a memory phenotype (CD8+CD44high) contained higher levels of Bcl-2 than the naive phenotype (CD8+CD44low), mirroring our observations with Ag-specific memory cells. Examination of CD4 naive and memory phenotype (CD4+CD44low vs CD4+CD44high) revealed no major differences between these two populations in Bcl-2 expression. This is consistent with the findings of Garcia et al. (20). Using transgenic CD4 T cells (specific to C5 complement protein), they monitored a CD4 response in vivo and found that Bcl-2 levels decrease in effector T cells and then return to normal levels in memory CD4 T cells (similar to those seen in naive cells). The difference in Bcl-2 expression between memory CD4 and CD8 T cells can be potentially explained through two mechanisms. Bcl-2 may be more of a critical survival factor for CD8 than CD4 T cells. Null mutants of the Bcl-2 gene lose most of their lymphocytes by the fourth week of life. Additionally, lymphocytes from these animals show impaired survival in vitro. Both of these defects are manifested more prominently in CD8 than in CD4 T cells (10). Another possible reason for the discrepancy in Bcl-2 levels is differences in cycling rate. As mentioned earlier, Bcl-2 is expressed more highly in Ag-specific memory than naive CD8 T cells. Memory CD8 T cells also have a higher rate of homeostatic proliferation than memory CD4 cells (K. Murali-Krishna and R. Ahmed, unpublished observations), suggesting a potential link between division rate in vivo and Bcl-2 expression. However, it should be noted that definitive analysis of Bcl-2 levels in Ag-specific memory CD4+ T cells will require the use of MHC class II tetramers to directly identify the Ag-specific cells.

Apoptosis is a complex multifactorial process controlled by interactions between pro- and anti-apoptotic genes. In this study, we have focused on the expression of one of the most important genes controlling apoptosis but future studies are needed to understand the roles of other genes such as Bcl-x, Bad, and Bax in controlling CD8 responses. We observed that Ag-specific memory cells contain increased Bcl-2, although effector cells are Bcl-2low. Understanding the mechanism by which a population of cells expressing high levels of Bcl-2 (memory) is generated from cells with low levels (effector) is a current endeavor in our laboratory. In addition, determining whether Bcl-2 is necessary for the generation and maintenance of CD8 memory T cells will require the generation of chimeras with CD8 T cells that lack Bcl-2. Finally, further studies are needed to determine whether the higher levels of Bcl-2 found in memory cells renders them more resistant than naive cells to apoptotic stimuli.


   Footnotes
 
1 This research was supported by National Institutes of Health Grants AI30048 (to R.A.) and NS21496 (to R. A.). A.J.Z. was supported by Damon Runyon Foundation postdoctoral fellowship 1DRG1421. J.M.G. was supported by National Research Service Award 1F32AI0249-01A1. Back

2 Current address: Department of Microbiology, University of Alabama, Birmingham, AL 35294. Back

3 Address correspondence and reprint requests to Dr. Rafi Ahmed, Emory University School of Medicine, G211 Rollins Research Building, 1510 Clifton Road, Atlanta, GA 30322. E-mail address: Back

4 Abbreviation used in this paper: LCMV, lymphocytic choriomeningitis virus. Back

Received for publication January 7, 2000. Accepted for publication February 17, 2000.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

  1. Murali-Krishna, K., J. D. Altman, M. Suresh, D. J. Sourdive, A. J. Zajac, J. D. Miller, J. Slansky, R. Ahmed. 1998. Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection. Immunity 8:177.[Medline]
  2. Dutton, R. W., L. M. Bradley, S. L. Swain. 1998. T cell memory. Annu. Rev. Immunol. 16:201.[Medline]
  3. Murali-Krishna, K., L. L. Lau, S. Sambhara, F. Lemonnier, J. Altman, R. Ahmed. 1999. Persistence of memory CD8 T cells in MHC class I-deficient mice. Science 286:1377.[Abstract/Free Full Text]
  4. Tanchot, C., F. A. Lemonnier, B. Perarnau, A. A. Freitas, B. Rocha. 1997. Differential requirements for survival and proliferation of CD8 naive or memory T cells. Science 276:2057.[Abstract/Free Full Text]
  5. Tough, D. F., J. Sprent. 1994. Turnover of naive- and memory-phenotype T cells. J. Exp. Med. 179:1127.[Abstract/Free Full Text]
  6. Adams, J. M., S. Cory. 1998. The Bcl-2 protein family: arbiters of cell survival. Science 281:1322.[Abstract/Free Full Text]
  7. Cleary, M. L., S. D. Smith, J. Sklar. 1986. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl- 2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell 47:19.[Medline]
  8. Yang, J., X. Liu, K. Bhalla, C. N. Kim, A. M. Ibrado, J. Cai, T. I. Peng, D. P. Jones, X. Wang. 1997. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science 275:1129.[Abstract/Free Full Text]
  9. Veis, D. J., C. M. Sorenson, J. R. Shutter, S. J. Korsmeyer. 1993. Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair. Cell 75:229.[Medline]
  10. Nakayama, K., I. Negishi, K. Kuida, H. Sawa, D. Y. Loh. 1994. Targeted disruption of Bcl-2 {alpha}ß in mice: occurrence of gray hair, polycystic kidney disease, and lymphocytopenia. Proc. Natl. Acad. Sci. USA 91:3700.[Abstract/Free Full Text]
  11. Broome, H. E., C. M. Dargan, S. Krajewski, J. C. Reed. 1995. Expression of Bcl-2, Bcl-x, and Bax after T cell activation and IL-2 withdrawal. J. Immunol. 155:2311.[Abstract]
  12. Ahmed, R., A. Salmi, L. D. Butler, J. M. Chiller, M. B. Oldstone. 1984. Selection of genetic variants of lymphocytic choriomeningitis virus in spleens of persistently infected mice: role in suppression of cytotoxic T lymphocyte response and viral persistence. J. Exp. Med. 160:521.[Abstract/Free Full Text]
  13. Zimmerman, C., K. Brduscha-Riem, C. Blaser, R. M. Zinkernagel, H. Pircher. 1996. Visualization, characterization, and turnover of CD8+ memory T cells in virus-infected hosts. J. Exp. Med. 183:1367.[Abstract/Free Full Text]
  14. Mazel, S., D. Burtrum, H. T. Petrie. 1996. Regulation of cell division cycle progression by bcl-2 expression: a potential mechanism for inhibition of programmed cell death. J. Exp. Med. 183:2219.[Abstract/Free Full Text]
  15. Lissy, N. A., L. F. Van Dyk, M. Becker-Hapak, A. Vocero-Akbani, J. H. Mendler, S. F. Dowdy. 1998. TCR antigen-induced cell death occurs from a late G1 phase cell cycle check point. Immunity 8:57.[Medline]
  16. Mueller, D. L., S. Seiffert, W. Fang, T. W. Behrens. 1996. Differential regulation of bcl-2 and bcl-x by CD3, CD28, and the IL-2 receptor in cloned CD4+ helper T cells: a model for the long-term survival of memory cells. J. Immunol. 156:1764.[Abstract]
  17. Shinohara, S., T. Sawada, Y. Nishioka, S. Tohma, T. Kisaki, T. Inoue, K. Ando, M. Ikeda, H. Fujii, K. Ito. 1995. Differential expression of Fas antigen and Bcl-2 protein on CD4+ T cells, CD8+ T cells, and monocytes. Cell Immunol. 163:303.[Medline]
  18. Akbar, A. N., N. Borthwick, M. Salmon, W. Gombert, M. Bofill, N. Shamsadeen, D. Pilling, S. Pett, J. E. Grundy, G. Janossy. 1993. The significance of low bcl-2 expression by CD45RO T cells in normal individuals and patients with acute viral infections: the role of apoptosis in T cell memory. J. Exp. Med. 178:427.[Abstract/Free Full Text]
  19. Cossarizza, A., C. Mussini, N. Mongiardo, V. Borghi, A. Sabbatini, B. De Rienzo, C. Franceschi. 1997. Mitochondria alterations and dramatic tendency to undergo apoptosis in peripheral blood lymphocytes during acute HIV syndrome. AIDS 11:19.[Medline]
  20. Garcia, S., J. DiSanto, B. Stockinger. 1999. Following the development of CD4 T cell response in vivo: from activation to memory formation. Immunity 11:163.[Medline]



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 J. Virol.Home page
J. J. Zaunders, S. Ip, M. L. Munier, D. E. Kaufmann, K. Suzuki, C. Brereton, S. C. Sasson, N. Seddiki, K. Koelsch, A. Landay, et al.
Infection of CD127+ (Interleukin-7 Receptor+) CD4+ Cells and Overexpression of CTLA-4 Are Linked to Loss of Antigen-Specific CD4 T Cells during Primary Human Immunodeficiency Virus Type 1 Infection.
J. Virol., October 1, 2006; 80(20): 10162 - 10172.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
P. Guillaume, P. Baumgaertner, G. S. Angelov, D. Speiser, and I. F. Luescher
Fluorescence-Activated Cell Sorting and Cloning of Bona Fide CD8+ CTL with Reversible MHC-Peptide and Antibody Fab' Conjugates
J. Immunol., September 15, 2006; 177(6): 3903 - 3912.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. M. Grayson, A. E. Weant, B. C. Holbrook, and D. Hildeman
Role of Bim in Regulating CD8+ T-Cell Responses during Chronic Viral Infection.
J. Virol., September 1, 2006; 80(17): 8627 - 8638.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H.-G. Zhang, C. Liu, K. Su, S. Yu, L. Zhang, S. Zhang, J. Wang, X. Cao, W. Grizzle, and R. P. Kimberly
A Membrane Form of TNF-{alpha} Presented by Exosomes Delays T Cell Activation-Induced Cell Death.
J. Immunol., June 15, 2006; 176(12): 7385 - 7393.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Bianchi, S. Gasser, A. Trumpp, and H. R. MacDonald
c-Myc acts downstream of IL-15 in the regulation of memory CD8 T-cell homeostasis
Blood, May 15, 2006; 107(10): 3992 - 3999.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Su, D. S. Chang, S. S. Gambhir, and J. Braun
Monitoring the Antitumor Response of Naive and Memory CD8 T Cells in RAG1-/- Mice by Positron-Emission Tomography
J. Immunol., April 1, 2006; 176(7): 4459 - 4467.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Migliaccio, P. M. S. Alves, P. Romero, and N. Rufer
Distinct Mechanisms Control Human Naive and Antigen-Experienced CD8+ T Lymphocyte Proliferation
J. Immunol., February 15, 2006; 176(4): 2173 - 2182.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Homann, W. Dummer, T. Wolfe, E. Rodrigo, A. N. Theofilopoulos, M. B. A. Oldstone, and M. G. von Herrath
Lack of Intrinsic CTLA-4 Expression Has Minimal Effect on Regulation of Antiviral T-Cell Immunity
J. Virol., January 1, 2006; 80(1): 270 - 280.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Bosco, F. Agenes, and R. Ceredig
Effects of Increasing IL-7 Availability on Lymphocytes during and after Lymphopenia-Induced Proliferation
J. Immunol., July 1, 2005; 175(1): 162 - 170.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. R. Sloma, M. J. Hansen, A. A. MacDougall, V. P. Van Keulen, R. B. Jenkins, and L. R. Pease
A Class I Transgene Reveals Regulatory Events on Chromosome 1 Marking Peripheral T Cell Differentiation and Memory
J. Immunol., June 15, 2005; 174(12): 7564 - 7572.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Cebecauer, P. Guillaume, P. Hozak, S. Mark, H. Everett, P. Schneider, and I. F. Luescher
Soluble MHC-Peptide Complexes Induce Rapid Death of CD8+ CTL
J. Immunol., June 1, 2005; 174(11): 6809 - 6819.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Zhang and Y.-W. He
The Antiapoptotic Protein Bcl-xL Is Dispensable for the Development of Effector and Memory T Lymphocytes
J. Immunol., June 1, 2005; 174(11): 6967 - 6973.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. C. Straathof, M. A. Pule, P. Yotnda, G. Dotti, E. F. Vanin, M. K. Brenner, H. E. Heslop, D. M. Spencer, and C. M. Rooney
An inducible caspase 9 safety switch for T-cell therapy
Blood, June 1, 2005; 105(11): 4247 - 4254.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Takahashi, M. G. V. Hanson, H. R. Norell, A. M. Havelka, K. Kono, K.-J. Malmberg, and R. V. R. Kiessling
Preferential Cell Death of CD8+ Effector Memory (CCR7-CD45RA-) T Cells by Hydrogen Peroxide-Induced Oxidative Stress
J. Immunol., May 15, 2005; 174(10): 6080 - 6087.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. S. Patke and D. L. Farber
Modulation of Memory CD4 T Cell Function and Survival Potential by Altering the Strength of the Recall Stimulus
J. Immunol., May 1, 2005; 174(9): 5433 - 5443.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
J. K. Whitmire, J. T. Tan, and J. L. Whitton
Interferon-{gamma} acts directly on CD8+ T cells to increase their abundance during virus infection
J. Exp. Med., April 4, 2005; 201(7): 1053 - 1059.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Bosque, J. Pardo, M{a} J. Martinez-Lorenzo, M. Iturralde, I. Marzo, A. Pineiro, M{a} A. Alava, J. Naval, and A. Anel
Down-regulation of normal human T cell blast activation: roles of APO2L/TRAIL, FasL, and c- FLIP, Bim, or Bcl-x isoform expression
J. Leukoc. Biol., April 1, 2005; 77(4): 568 - 578.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Charo, S. E. Finkelstein, N. Grewal, N. P. Restifo, P. F. Robbins, and S. A. Rosenberg
Bcl-2 Overexpression Enhances Tumor-Specific T-Cell Survival
Cancer Res., March 1, 2005; 65(5): 2001 - 2008.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Z. Dai, I. W. Nasr, M. Reel, S. Deng, L. Diggs, C. P. Larsen, D. M. Rothstein, and F. G. Lakkis
Impaired Recall of CD8 Memory T Cells in Immunologically Privileged Tissue
J. Immunol., February 1, 2005; 174(3): 1165 - 1170.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Giroux and F. Denis
CD1d-unrestricted human NKT cells release chemokines upon Fas engagement
Blood, January 15, 2005; 105(2): 703 - 710.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Suresh, A. Singh, and C. Fischer
Role of Tumor Necrosis Factor Receptors in Regulating CD8 T-Cell Responses during Acute Lymphocytic Choriomeningitis Virus Infection
J. Virol., January 1, 2005; 79(1): 202 - 213.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Ramakrishna, S. A. Stohlman, R. A. Atkinson, D. R. Hinton, and C. C. Bergmann
Differential Regulation of Primary and Secondary CD8+ T Cells in the Central Nervous System
J. Immunol., November 15, 2004; 173(10): 6265 - 6273.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. J. Wherry, D. L. Barber, S. M. Kaech, J. N. Blattman, and R. Ahmed
Antigen-independent memory CD8 T cells do not develop during chronic viral infection
PNAS, November 9, 2004; 101(45): 16004 - 16009.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Roychowdhury, K. F. May Jr., K. S. Tzou, T. Lin, D. Bhatt, A. G. Freud, M. Guimond, A. K. Ferketich, Y. Liu, and M. A. Caligiuri
Failed Adoptive Immunotherapy with Tumor-Specific T Cells: Reversal with Low-Dose Interleukin 15 but not Low-Dose Interleukin 2
Cancer Res., November 1, 2004; 64(21): 8062 - 8067.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Sabbagh, S. M. Kaech, M. Bourbonniere, M. Woo, L. Y. Cohen, E. K. Haddad, N. Labrecque, R. Ahmed, and R.-P. Sekaly
The Selective Increase in Caspase-3 Expression in Effector but Not Memory T Cells Allows Susceptibility to Apoptosis
J. Immunol., November 1, 2004; 173(9): 5425 - 5433.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. A. Corbin and J. T. Harty
Duration of Infection and Antigen Display Have Minimal Influence on the Kinetics of the CD4+ T Cell Response to Listeria monocytogenes Infection
J. Immunol., November 1, 2004; 173(9): 5679 - 5687.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. G. Laniewski and J. M. Grayson
Antioxidant Treatment Reduces Expansion and Contraction of Antigen-Specific CD8+ T Cells during Primary but Not Secondary Viral Infection
J. Virol., October 15, 2004; 78(20): 11246 - 11257.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
X. Z. Wang, M. A. Brehm, and R. M. Welsh
Preapoptotic Phenotype of Viral Epitope-Specific CD8 T Cells Precludes Memory Development and Is an Intrinsic Property of the Epitope
J. Immunol., October 15, 2004; 173(8): 5138 - 5147.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Dondi, G. Roue, V. J. Yuste, S. A. Susin, and S. Pellegrini
A Dual Role of IFN-{alpha} in the Balance between Proliferation and Death of Human CD4+ T Lymphocytes during Primary Response
J. Immunol., September 15, 2004; 173(6): 3740 - 3747.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. J. Obar, S. G. Crist, E. K. Leung, and E. J. Usherwood
IL-15-Independent Proliferative Renewal of Memory CD8+ T Cells in Latent Gammaherpesvirus Infection
J. Immunol., August 15, 2004; 173(4): 2705 - 2714.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. H. Robbins, M. S. Tessmer, T. Mikayama, and L. Brossay
Expansion and Contraction of the NK Cell Compartment in Response to Murine Cytomegalovirus Infection
J. Immunol., July 1, 2004; 173(1): 259 - 266.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. C. Lenz, S. K. Kurz, E. Lemmens, S. P. Schoenberger, J. Sprent, M. B. A. Oldstone, and D. Homann
IL-7 regulates basal homeostatic proliferation of antiviral CD4+T cell memory
PNAS, June 22, 2004; 101(25): 9357 - 9362.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. J. Wherry and R. Ahmed
Memory CD8 T-Cell Differentiation during Viral Infection
J. Virol., June 1, 2004; 78(11): 5535 - 5545.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Zhu, B. J. Swanson, M. Wang, D. A. Hildeman, B. C. Schaefer, X. Liu, H. Suzuki, K. Mihara, J. Kappler, and P. Marrack
Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells
PNAS, May 18, 2004; 101(20): 7681 - 7686.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Dooms, E. Kahn, B. Knoechel, and A. K. Abbas
IL-2 Induces a Competitive Survival Advantage in T Lymphocytes
J. Immunol., May 15, 2004; 172(10): 5973 - 5979.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Th. den Boer, G. J. D. van Mierlo, M. F. Fransen, C. J. M. Melief, R. Offringa, and R. E. M. Toes
The Tumoricidal Activity of Memory CD8+ T Cells Is Hampered by Persistent Systemic Antigen, but Full Functional Capacity Is Regained in an Antigen-Free Environment
J. Immunol., May 15, 2004; 172(10): 6074 - 6079.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
P. Pandiyan, D. Gartner, O. Soezeri, A. Radbruch, K. Schulze-Osthoff, and M. C. Brunner-Weinzierl
CD152 (CTLA-4) Determines the Unequal Resistance of Th1 and Th2 Cells against Activation-induced Cell Death by a Mechanism Requiring PI3 Kinase Function
J. Exp. Med., March 15, 2004; 199(6): 831 - 842.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Tewari, J. Sacha, X. Gao, and M. Suresh
Effect of Chronic Viral Infection on Epitope Selection, Cytokine Production, and Surface Phenotype of CD8 T Cells and the Role of IFN-{gamma} Receptor in Immune Regulation
J. Immunol., February 1, 2004; 172(3): 1491 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
E. L. Oleszak, J. R. Chang, H. Friedman, C. D. Katsetos, and C. D. Platsoucas
Theiler's Virus Infection: a Model for Multiple Sclerosis
Clin. Microbiol. Rev., January 1, 2004; 17(1): 174 - 207.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. V. D. Soares, F. J. Plunkett, C. S. Verbeke, J. E. Cook, J. M. Faint, L. L. Belaramani, J. M. Fletcher, N. Hammerschmitt, M. Rustin, W. Bergler, et al.
Integration of apoptosis and telomere erosion in virus-specific CD8+ T cells from blood and tonsils during primary infection
Blood, January 1, 2004; 103(1): 162 - 167.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ilangumaran, S. Ramanathan, J. La Rose, P. Poussier, and R. Rottapel
Suppressor of Cytokine Signaling 1 Regulates IL-15 Receptor Signaling in CD8+CD44high Memory T Lymphocytes
J. Immunol., September 1, 2003; 171(5): 2435 - 2445.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Grayson, N. G. Laniewski, J. G. Lanier, and R. Ahmed
Mitochondrial Potential and Reactive Oxygen Intermediates in Antigen-Specific CD8+ T Cells During Viral Infection
J. Immunol., May 1, 2003; 170(9): 4745 - 4751.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. M. Kerksiek, A. Ploss, I. Leiner, D. H. Busch, and E. G. Pamer
H2-M3-Restricted Memory T Cells: Persistence and Activation Without Expansion
J. Immunol., February 15, 2003; 170(4): 1862 - 1869.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Kelly, R. Spolski, K. Imada, J. Bollenbacher, S. Lee, and W. J. Leonard
A Role for Stat5 in CD8+ T Cell Homeostasis
J. Immunol., January 1, 2003; 170(1): 210 - 217.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Rubinstein, A. N. Kadima, M. L. Salem, C. L. Nguyen, W. E. Gillanders, and D. J. Cole
Systemic Administration of IL-15 Augments the Antigen-Specific Primary CD8+ T Cell Response Following Vaccination with Peptide-Pulsed Dendritic Cells
J. Immunol., November 1, 2002; 169(9): 4928 - 4935.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H.-H. Xue, P. E. Kovanen, C. A. Pise-Masison, M. Berg, M. F. Radovich, J. N. Brady, and W. J. Leonard
IL-2 negatively regulates IL-7 receptor alpha chain expression in activated T lymphocytes
PNAS, October 15, 2002; 99(21): 13759 - 13764.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Pantenburg, F. Heinzel, L. Das, P. S. Heeger, and A. Valujskikh
T Cells Primed by Leishmania major Infection Cross-React with Alloantigens and Alter the Course of Allograft Rejection
J. Immunol., October 1, 2002; 169(7): 3686 - 3693.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Grayson, L. E. Harrington, J. G. Lanier, E. J. Wherry, and R. Ahmed
Differential Sensitivity of Naive and Memory CD8+ T Cells to Apoptosis in Vivo
J. Immunol., October 1, 2002; 169(7): 3760 - 3770.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. J. Dunne, J. M. Faint, N. H. Gudgeon, J. M. Fletcher, F. J. Plunkett, M. V. D. Soares, A. D. Hislop, N. E. Annels, A. B. Rickinson, M. Salmon, et al.
Epstein-Barr virus-specific CD8+ T cells that re-express CD45RA are apoptosis-resistant memory cells that retain replicative potential
Blood, July 18, 2002; 100(3): 933 - 940.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y. Nakamoto, S. Kaneko, and K. Kobayashi
Increased susceptibility to apoptosis and attenuated Bcl-2 expression in T lymphocytes and monocytes from patients with advanced chronic hepatitis C
J. Leukoc. Biol., July 1, 2002; 72(1): 49 - 55.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. M. Onami, L. E. Harrington, M. A. Williams, M. Galvan, C. P. Larsen, T. C. Pearson, N. Manjunath, L. G. Baum, B. D. Pearce, and R. Ahmed
Dynamic Regulation of T Cell Immunity by CD43
J. Immunol., June 15, 2002; 168(12): 6022 - 6031.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. P. Davenport, C. Fazou, A. J. McMichael, and M. F. C. Callan
Clonal Selection, Clonal Senescence, and Clonal Succession: The Evolution of the T Cell Response to Infection with a Persistent Virus
J. Immunol., April 1, 2002; 168(7): 3309 - 3317.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
V. Appay, L. Papagno, C. A. Spina, P. Hansasuta, A. King, L. Jones, G. S. Ogg, S. Little, A. J. McMichael, D. D. Richman, et al.
Dynamics of T Cell Responses in HIV Infection
J. Immunol., April 1, 2002; 168(7): 3660 - 3666.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Harbertson, E. Biederman, K. E. Bennett, R. M. Kondrack, and L. M. Bradley
Withdrawal of Stimulation May Initiate the Transition of Effector to Memory CD4 Cells
J. Immunol., February 1, 2002; 168(3): 1095 - 1102.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Yajima, H. Nishimura, R. Ishimitsu, T. Watase, D. H. Busch, E. G. Pamer, H. Kuwano, and Y. Yoshikai
Overexpression of IL-15 In Vivo Increases Antigen-Driven Memory CD8+ T Cells Following a Microbe Exposure
J. Immunol., February 1, 2002; 168(3): 1198 - 1203.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
X. Zhang, H. Fujii, H. Kishimoto, E. LeRoy, C. D. Surh, and J. Sprent
Aging Leads to Disturbed Homeostasis of Memory Phenotype CD8+ Cells
J. Exp. Med., January 28, 2002; 195(3): 283 - 293.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Zhou, R. Ou, L. Huang, and D. Moskophidis
Critical Role for Perforin-, Fas/FasL-, and TNFR1-Mediated Cytotoxic Pathways in Down-Regulation of Antigen-Specific T Cells during Persistent Viral Infection
J. Virol., January 15, 2002; 76(2): 829 - 840.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T.-S. Wu, J.-M. Lee, Y.-G. Lai, J.-C. Hsu, C.-Y. Tsai, Y.-H. Lee, and N.-S. Liao
Reduced Expression of Bcl-2 in CD8+ T Cells Deficient in the IL-15 Receptor {alpha}-Chain
J. Immunol., January 15, 2002; 168(2): 705 - 712.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Szmania, A. Galloway, M. Bruorton, P. Musk, G. Aubert, A. Arthur, H. Pyle, N. Hensel, N. Ta, L. Lamb Jr, et al.
Isolation and expansion of cytomegalovirus-specific cytotoxic T lymphocytes to clinical scale from a single blood draw using dendritic cells and HLA-tetramers
Blood, August 1, 2001; 98(3): 505 - 512.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Ohteki, C. Maki, and S. Koyasu
Overexpression of Bcl-2 Differentially Restores Development of Thymus-Derived CD4-8+ T Cells and Intestinal Intraepithelial T Cells in IFN-Regulatory Factor-1-Deficient Mice
J. Immunol., June 1, 2001; 166(11): 6509 - 6513.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Y. Choi, Y. Yoshimura, G. J. Christianson, T. J. Sproule, S. Malarkannan, N. Shastri, S. Joyce, and D. C. Roopenian
Quantitative Analysis of the Immune Response to Mouse Non-MHC Transplantation Antigens In Vivo: The H60 Histocompatibility Antigen Dominates Over All Others
J. Immunol., April 1, 2001; 166(7): 4370 - 4379.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. T. Young, M. Uhrberg, J. H. Phillips, L. L. Lanier, and P. Parham
Differential Expression of Leukocyte Receptor Complex-Encoded Ig-Like Receptors Correlates with the Transition from Effector to Memory CTL
J. Immunol., March 15, 2001; 166(6): 3933 - 3941.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. J. Plunkett, M. V. D. Soares, N. Annels, A. Hislop, K. Ivory, M. Lowdell, M. Salmon, A. Rickinson, and A. N. Akbar
The flow cytometric analysis of telomere length in antigen-specific CD8+ T cells during acute Epstein-Barr virus infection
Blood, February 1, 2001; 97(3): 700 - 707.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Grayson, K. Murali-Krishna, J. D. Altman, and R. Ahmed
Gene Expression in Antigen-Specific CD8+ T Cells During Viral Infection
J. Immunol., January 15, 2001; 166(2): 795 - 799.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Holtappels, M.-F. Pahl-Seibert, D. Thomas, and M. J. Reddehase
Enrichment of Immediate-Early 1 (m123/pp89) Peptide-Specific CD8 T Cells in a Pulmonary CD62Llo Memory-Effector Cell Pool during Latent Murine Cytomegalovirus Infection of the Lungs
J. Virol., December 15, 2000; 74(24): 11495 - 11503.
[Abstract] [Full Text]

Home page
 BloodHome page
P. Shankar, M. Russo, B. Harnisch, M. Patterson, P. Skolnik, and J. Lieberman
Impaired function of circulating HIV-specific CD8+ T cells in chronic human immunodeficiency virus infection
Blood, November 1, 2000; 96(9): 3094 - 3101.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
A. D. Hislop, N. E. Annels, N. H. Gudgeon, A. M. Leese, and A. B. Rickinson
Epitope-specific Evolution of Human CD8+ T Cell Responses from Primary to Persistent Phases of Epstein-Barr Virus Infection
J. Exp. Med., April 1, 2002; 195(7): 893 - 905.
[Abstract] [Full Text] [PDF]

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