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The Journal of Immunology, 2000, 164: 3193-3199.
Copyright © 2000 by The American Association of Immunologists

Unprecedented Polymorphism of Mhc-DRB Region Configurations in Rhesus Macaques1

Gaby G. M. Doxiadis2, Nel Otting, Natasja G. de Groot, Riet Noort and Ronald E. Bontrop

Department of Immunobiology, Biomedical Primate Research Centre, Rijswijk, The Netherlands


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results and Discussion
 References
 
The rhesus macaque is an important model in preclinical transplantation research and for the study of chronic and infectious diseases, and so extensive knowledge of its MHC (MhcMamu) is needed. Nucleotide sequencing of exon 2 allowed the detection of 68 Mamu-DRB alleles. Although most alleles belong to loci/lineages that have human equivalents, identical Mhc-DRB alleles are not shared between humans and rhesus macaques. The number of -DRB genes present per haplotype can vary from two to seven in the rhesus macaque, whereas it ranges from one to four in humans. Within a panel of 210 rhesus macaques, 24 Mamu-DRB region configurations can be distinguished differing in the number and composition of loci. None of the Mamu-DRB region configurations has been described for any other species, and only one of them displays major allelic variation giving rise to a total of 33 Mamu-DRB haplotypes. In the human population, only five HLA-DRB region configurations were defined, which in contrast to the rhesus macaque exhibit extensive allelic polymorphism. In comparison with humans, the unprecedented polymorphism of the Mamu-DRB region configurations may reflect an alternative strategy of this primate species to cope with pathogens. Because of the Mamu-DRB diversity, nonhuman primate colonies used for immunological research should be thoroughly typed to facilitate proper interpretation of results. This approach will minimize as well the number of animals necessary to conduct experiments.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results and Discussion
 References
 
Rhesus macaques are used as preclinical models for human infectious diseases (AIDS) and chronic disorders (multiple sclerosis, rheumatoid arthritis) and for transplantation biology (1, 2, 3, 4, 5, 6, 7, 8, 9). The MHC gene products play a key role in adaptive immunology, and two classes are distinguished, namely, class I and class II. MHC class II gene products are normally expressed on white blood cells and act as cell surface receptors for processed peptides derived from an extracellular origin. MHC class II molecules play a pivotal role in T cell recognition of foreign and self-Ags. Activation of T cells, which recognize self-peptides in the context of MHC molecules, is thought to be a risk factor which may result in autoimmune diseases. For instance, experimental autoimmune encephalomyelitis in rhesus macaques, a model for the human disease multiple sclerosis, is known to be influenced by MHC class II region factors (4). Furthermore, a mismatch for allogeneic MHC class I and/or II molecules may lead to an accelerated allograft rejection or graft-vs-host disease after transplantation (10).

The HLA class II region is divided into -DP, -DQ, and -DR. The classical HLA-DR, -DQ, and -DP molecules are transmembrane heterodimers, composed of an {alpha}- and ß-chain subunit encoded by the A and B genes, respectively. The key feature of the MHC complex is its high degree of polymorphism. Most sequence variability is confined to exon 2 of the Mhc-DPB, -DQA, -DQB, and -DRB genes, whereas the most polymorphic region in humans is the HLA-DRB region with 271 alleles (11). Humans, apes, and Old World monkeys share most Mhc class II loci (12, 13, 14, 15) but also many lineages that predate the speciation of the contemporary living primates (16, 17). These high degrees of sequence similarity can have functional implications, as has been demonstrated by the presentation of peptides across a species barrier (18).

In the human population, five major -DRB region configurations are classified. These regions share an invariant HLA-DRA and a -DRB9 gene segment but differ in physical length and also in the composition of loci. Like humans, chimpanzees, gorillas, and rhesus macaques have variable numbers of Mhc-DRB loci per haplotype (19, 20, 21, 22, 23, 24). Previous analyses involving a limited number of consanguineous rhesus macaques, known to be homozygous for their MHC region, indicated that the number of Mamu-DRB loci per haplotype varies from two to six with up to three -DRB genes expressed (25). In the present study, we report on the number of Mamu-DRB region configurations and haplotypes that can be observed in the Biomedical Primate Research Centre’s (BPRC)3 breeding colony. In this context, a Mamu-DRB haplotype is defined as the combination of different alleles present in a cis configuration within the Mamu-DR region. Mhc-DRB haplotypes, which consist of the same number and content of -DRB genes, are called -DRB region configurations. BPRC’s breeding colony of rhesus macaques comprises monkeys mainly from India with a small number of animals originating from China and Burma. The colony has been pedigreed based on segregation of serologically defined Mamu-A, -B, and -DR Ags, and 10 Mamu-DR specificities have been defined (26). Subsequently, a large segment of the BPRC breeding colony has been analyzed by molecular techniques as denaturing gradient gel electrophoresis (DGGE) to screen for Mamu-DRB exon 2 variation (27). Animals that show unknown DGGE profiles were subjected to nucleotide sequence analysis. At present, 116 Mamu-DRB alleles have been characterized, and 68 of them are discussed in this study (14, 25, 27, 28, 29, 30). Most of the alleles belong to loci/lineages that are shared between humans and macaques (17). These are the Mhc-DRB1*03, -DRB1*10, -DRB1*04, and -DRB1*07 lineages and the Mhc-DRB loci -DRB3, -DRB4, -DRB5, and -DRB6. For some loci/lineages, no human equivalents have been identified. Those are designated by workshop (W) numbers, namely, -DRB*W1–DRB*W7, -DRB*W20, -DRB*W21, -DRB*W25–DRB*W28, and -DRB*W31. The W designation indicates that it is not yet known whether these lineages actually represent different loci. Extensive segregation studies of Mamu-DRB polymorphisms in pedigreed rhesus monkey families allowed the determination of a large number of Mamu-DRB region configurations and haplotypes.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results and Discussion
 References
 
Animals

In this study, 210 rhesus macaques belonging to the BPRC self-sustaining colony were analyzed. All animals were serologically typed for their Mamu-A, -B, and -DR Ags, and by pedigree analysis over four generations’ haplotypes could be determined. Selective breeding (consanguineous matings) permitted the establishment of a panel of monkeys fully homozygous for their Mamu-A, -B, and -DR Ags which were used as homozygous typing cells.

DNA isolation and PCR

Genomic DNA was extracted from heparinized blood samples or from immortalized B cells of the rhesus macaques. PCR of exon 2 of Mamu-DRB was conducted with generic primers as previously described (27) (N. Otting, N. G. de Groot, M. C, Noort, G. G. M. Doxiadis, and R. E. Bontrop, manuscript in preparation). For amplification with sequence-specific primers (SSP) of Mamu-DRB6 genes with the 62-bp deletion (25), the following primers were used with restriction sites for SalI and XbaI, respectively: (5'-MDRB6) 5'-TTG GTC GAC GCT AAG TGY GAS TGT CMT A-3' and (3'-MDRB6) 5'-CTC TCT AGA CCS RYA ATT GTA AYT CTG T-3'.

Denaturing gradient gel electrophoresis

Separation of Mamu-DRB alleles with DGGE was conducted as described, with minor modifications (27). Briefly, GC-clamped PCR products of DRB exon 2 were electrophoresed in a 9% acrylamide (acrylamide-bisacrylamide, 37.5:1) gel with a 40–65% parallel denaturing gradient of urea and formamide at a constant temperature of 57°C for ~3.5 h. When DNA fragments are electrophoresed through an increasing gradient of denaturants, the alleles dissociate at different rates because of sequence-specific differences in denaturation. As a consequence, each allele migrates at a unique rate through the gel. Alleles could be defined in a parallel run with already described banding patterns and/or in comparison with reamplified ssDNA clones of known content. Bands that could not be identified in the manner described above were cut out of the gel; then the DNA was eluted, reamplified, and subjected to direct sequencing (27).

Cloning and sequencing

M13 cloning and sequencing (31) as well as direct sequencing was achieved as described before on a Perkin-Elmer ABI Prism 310 genetic analyzer with the use of ABI PRISM dRhodamine Terminator Cycle Sequencing Ready Reaction Kit according to the manufacturer’s instructions.

Mamu-DRB6 typing by sequence-specific oligotyping (SSO) and sequencing

SSO was used to descriminate the Mamu-DRB6 alleles containing a 62-bp deletion (25) according to the method described before (32). The biotinylated oligonucleotides used were the following: Mamu-DRB6*0102 (triplet (tr) 47–52), 5'-TT CCA GGA GGT GAG GGA A-3'; Mamu-DRB6*0103 (tr 37–41), 5'-AA CCT GGT CTT CCA C-3'; Mamu-DRB6*0104A (tr 38–42), 5'-CT GCG CTA CAA CAG C-3'; Mamu-DRB6*0104B (insert), 5'-GG GAG GAG AAC CCT G-3'; Mamu-DRB6*0104/0105 (tr 53–57), 5'-CT GGGGTG GCC TGT C-3'; Mamu-DRB6*0105 (tr 39–44), 5'-CG CTT CCA CAG CGA CCT G-3'; Mamu-DRB6* 0106 (tr 49–53), 5'-GC GGT GAT GGA ACT G-3'; Mamu-DRB6*0107 (tr 21–25), 5'-TC TGA GCA GGT GCA G-3'; Mamu-DRB6*0115 (tr 32–36), 5'-CA TAA ACA GGA GGA G-3'. The biotinylated oligonucleotide detecting all Mamu-DRB6 alleles with deletion was 5'-CC TGT CAC AGA RTT ACA ATT-3'.

In the case of ambiguous SSO typing results, direct sequencing or M13 cloning and sequencing of the -DRB6-specific PCR products of exon 2 was performed as described above.

Nomenclature of Mamu-DRB alleles

The allocation of Mhc class II alleles to a certain lineage is based on the similarity of nucleotide sequence motifs within the first part of exon 2 and/or shared clustering in phylogenetic trees. Mamu-DRB alleles that cluster within lineages present in humans and macaques are depicted by the same lineage numbers. The last two or three digits are arbitrary and reflect the order in which the alleles were detected. Alleles with three digits after the lineage number differ from each other only by a synonymous substitution. Loci/lineages for which no apparent human equivalent has been identified are designated by workshop numbers. Alleles that do not cluster within any known equivalent as Mamu-DRB*W2601 and Mamu-DRB*W2701/02 receive a new lineage number.


   Results and Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
References
 
Mamu-DRB typing and molecular characterization

Serology. Alloantisera allow the serological definition of 13 Mamu-A, 13 Mamu-B, and 9 Mamu-DR specificities (15). The Mamu-DR specificities have been designated Mamu-DR1 to -DR8 and -DR101. Furthermore, another -DR group represents so-called serological "blank" specificities because the corresponding alloantisera are lacking. BPRC houses a self-sustaining colony of rhesus macaques, and four generations of animals have been born in captivity. The serological markers have been shown to segregate according to mendelian rules, and therefore haplotypes could be defined. Based on the combination of different serologically defined Mamu-A, -B, and -DR specificities, at least 264 haplotypes were observed within the breeding colony of ~800 animals.

DGGE. On the basis of experience in the HLA field, it is known that novel Mhc-DRB haplotypes are frequently detected in individuals that possess rare combinations of class I and class II seromarkers. For that reason, ~210 pedigreed monkeys with 190 different serologically defined haplotypes were selected to analyze Mamu-DRB region associated polymorphisms by means of DGGE. As in humans, the number of Mamu-DRB genes is not constant (25) and therefore Mamu-DRB DGGE profiles are complex (27). As reference markers, DNA from MHC homozygous typing cell lines (Fig. 1Go, lanes 2 and 4) was used in combination with DNA from animals with the same serotype (Fig. 1Go, lanes 1 and 10). This approach demonstrated that each Mamu-DR serotype represents multiple DGGE profiles. In the case of true Mamu-DR homozygous cell lines, the DGGE profile provides information on the number of Mamu-DRB genes present per haplotype (Fig. 1Go, lanes 2 and 4). If one analyzes Mamu-DR heterozygous animals, the number of Mamu-DRB genes present per haplotype can be deducted from the DGGE profile only if segregation analysis is performed or if DNA obtained from Mamu-DR reference cells is available. For only a few reference cell lines have particular DGGE profiles been matched with alleles (27). If a DGGE profile revealed an unknown pattern, the samples were subjected to sequence analysis to identify the Mamu-DRB alleles in question. In most cases, DGGE analysis and nucleotide sequencing provided unequivocal results. However, some alleles appear to be underrepresented in the PCR amplification and therefore may be missed in DGGE as well as in sequencing analysis. This is the case for some Mamu-DRB6 members (Mamu-DRB6*0102–Mamu-DRB6*0107) that share a deletion of 62 nucleotides spanning the position 181–242 (25). For those Mamu-DRB6 alleles, a specific PCR amplification was performed (SSP), and the PCR products were analyzed by either SSO or sequencing. An overview of the methodologies used to type the 68 Mamu-DRB alleles discussed in this study is given in Table IGo.



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  		FIGURE 1. PCR products of Mamu-DRB exon 2 separated by DGGE. PCR products of -DRB exon 2 of 10 different rhesus macaques separated in DGGE are shown in lanes 1–10. Lane 2, -DRB alleles -DRB6*0101,- DRB1*0309, -DRB*W201 of a Mamu-DR 3,3 homozygous typing cell; lane 4, -DRB alleles -DRB1*0303, -DRB1*1007 of a Mamu-DR1,1 homozygous typing cell.

 

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  		Table I. Overview of the technologies used for determination of the Mamu-DRB alleles

 
Analysis of Mamu-DRB haplotypes

From the 264 serologically defined Mamu-A, -B, and -DR haplotypes, 190 were tested for their -DRB content at the molecular level. A Mamu-DRB haplotype was defined when at least one of the following three criteria was met: 1) the rhesus macaque in question was serologically typed as Mamu-A, -B, and -DR homozygous and originates from consanguineous offspring (Fig. 1Go, lanes 2 and 4); 2) segregation of allelic polymorphism could be followed within a family; 3) the combination of -DRB alleles had been detected in several unrelated rhesus macaques, preferably with a shared serotype. An example is given in Fig. 1Go, where the -DRB DGGE pattern of three heterozygous rhesus macaques shown in lanes 1, 5, and 10 share the haplotype -DRB1*0303, -DRB1*1007 with the DR 1,1 homozygous monkey (Fig. 1Go, lane 4).

A total of 33 Mamu-DRB haplotypes can be differentiated which can be divided into 24 -DRB region configurations differing in their number and composition of loci. Table IIGo shows these Mamu-DR region configurations with their allelic haplotypes listed according to the number of Mamu-DRB genes detected. None of them is identical in its organization to an HLA-DRB equivalent. There are five region configurations comprising only two loci, whereas most of the -DRB region configurations consists of a combination of three or four -DRB genes. Only two -DRB region configurations could be observed with five -DRB loci, one with six and one with seven loci. By convention, in humans every HLA-DRB haplotype encodes one -DRB1 locus (11). This does not hold for the Mamu-DRB haplotypes. On one hand, there are several -DRB region configurations without a conventional -DRB1 locus, namely, groups 2c to 2e, 3f to 3h, 4f, 4g, and 5b. One of them, 2e, appears to have only two macaque-specific loci. On the other hand, there are -DRB groups, 2a, 3a, 3b, 4a, and 4b, encoding two Mamu-DRB1 genes which, however, belong to different lineages (Table IIGo). The Mamu-DRB1 loci/lineages are old entities predating the speciation of the contemporary living primates (17). As such, these loci may have been placed in a cis-configuration due to crossing-over effects (25).


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  		Table II. Mamu-DRB region configurations and their allelic haplotypes listed according to the number of loci present

 
Only one -DRB region configuration, 3a, displays extensive polymorphism, whereas all the others appear to be monomorphic or at best oligomorphic. Within the oligomorphic -DRB region configuration 3e, the haplotype -DRB1*0701, -DRB3*0405, -DRB5*0303 comes from rhesus macaques of Indian origin, whereas the allelic haplotype -DRB1*0702, -DRB3*0406, -DRB5*0305 is observed in a Chinese monkey (Table IIGo). Thus, allelic variation of -DRB haplotypes may reflect to a certain extent different origins of the rhesus macaques. Nevertheless, the -DRB region configuration, 3a, with a high degree of allelic variation and the invariant one, 3c, are both frequent in our colony. Therefore, the frequency of a given -DRB region configuration seems to be independent of its degree of allelic polymorphism.

The majority of -DRB region configurations share the presence of a Mamu-DRB6 gene (Table IIGo). Some of the apparently truncated -DRB regions without a -DRB6 gene (Table IIGo, 2a and 2e;) appear to be present in an extended configuration with an additional Mamu-DRB6 gene (Table IIGo, 3a and 3g;). The same holds for the -DRB region configurations 4a and 7, which are like 3a and 6, respectively, with a duplication of the -DRB6 gene.

The question arises whether all these genes are functional. For some loci, however, it is known that they encode gene products. In 2D gel electrophoresis the Mamu-DRB1*03, -DRB1*10, -DRB1*04, -DRB*W3, -DRB*W4, and -DRB*W5 were shown to encode DR molecules (17, 33). In vitro studies revealed that gene products of -DRB1*03, -DR1*04, and -DRB*W201 are able to present peptides to CD4+ cells, the latter being significant as restriction elements in cellular response to conserved regions of the HIV (18, 34). Mamu-DRB3, -DRB4, -DRB5, and -DRB*W101 gene products are also present on the cell surface and may have a role in the alloimmune proinflammatory cytokine response (35). These results indicate that at least 11 Mamu-DRB loci/lineages are important for the immune response.

Conservation of the Mhc-DRB6 pseudogene

The HLA- and Patr-DRB6 genes are considered to be pseudogenes because they lack exon 1 coding for the leader peptide of the corresponding gene product. Moreover, these genes share other features such as premature stop codons that are thought to interfere with proper translation. Some studies have documented, however, the presence of mRNA. This can be explained by the integration of a retroviral insert the long terminal repeat of which provides a promotor and a stretch of hydrophobic amino acids that could function as a leader (36). In addition, transcription and translation of human and chimpanzee -DRB6 genes could be demonstrated (37, 38). The Mamu-DRB6 exon 2 sequences are characterized by various characteristics such as inserts, stop codons, and deletions that would render it as a pseudogene. In this context, it is remarkable that nearly all of the 24 different Mamu-DRB groups harbor at least one -DRB6 gene locus. The Mhc-DRB6 gene has probably been a pseudogene >58 million years (39). This would be consistent with the finding that a high number of Mamu-DRB6 alleles have been identified. An explanation for the maintenance of the -DRB6 gene could be that its gene product has a completely different function from the classical MHC class II gene products. One such possibility would be, for instance, that Mhc-DRB6-encoded peptide segments can be bound by other MHC class I and/or II molecules and as such play a key role in thymic eduction. This would be consistent with the fact that translation has been documented. An alternative explanation is that the Mhc-DRB6 locus is flanked by genes, which are under strong positive selection.

Interspecies comparisons

The primate species whose DR region has been studied most extensively is the human, and five major HLA-DRB region configurations have been defined by molecular mapping studies (11). In humans, the number of Mhc-DRB genes varies from one to four depending on the -DRB region configuration (Fig. 2Go). In a previous communication, the Mamu-DR region was reported to be subject to contraction and expansion and seven -DRB region configurations were recorded (25). In rhesus macaques the number of -DRB genes appears to vary from two to seven per haplotype (Table IIGo). The detailed study of a much larger group of rhesus macaques allowed the definition of 24 Mamu-DRB region configurations (Fig. 2Go). No other primate species thoroughly investigated at the population level, such as humans, chimpanzees, and common marmosets, has been documented to possess such a level of variation at the DR region with regard to gene content and make-up. This number of 24 -DRB region configurations seems to be only the tip of the iceberg, because not all -DRB lineages described, for example -DRB*W28 and-DRB*W31, and only 68 -DRB alleles of 116 reported have been allocated to a certain haplotype. Furthermore, five other Mamu-DRB region configurations that apparently are not present in our breeding colony have been defined by another research group (40), bringing the total number of distinct Mamu-DRB region configurations up to 29. For our analyses, monkeys mainly of Indian origin were used. It is anticipated that more Mamu-DRB region configurations will be detected when animals from other geographic areas are studied. The question arises why rhesus macaques may exhibit much more polymorphism concerning their Mhc-DRB region configurations than other primate species. An explanation would be that the older a species is, the more complex the haplotype organization may be. The modern human species is considered to be <250,000 years old, whereas rhesus macaques diverged from other macaque species >700,000 years ago (41, 42). Based on these data, the time period to generate different -DRB region configurations was 3 times as long for the rhesus macaques as for humans. A second explanation is that distinct species utilize different strategies to cope with pathogens. The primate Mhc-DR region is unique with regard to its variation in gene numbers, and allelic variation adds an extra level of complexity. Some primate species such as humans have a low number of -DRB region configurations but display a high level of allelic heterogeneity, e.g., the HLA-DR53 region, comprising 39 alleles (11). Rhesus macaques, on the contrary, have a high number of -DRB region configurations, but allelic variation appears to be relatively low. With regard to allelic variation, the DR region of the chimpanzee seems to mimic the human situation, and until now nine DR region configurations have been detected. Hence, one would predict that primate species possessing limited or no polymorphism of -DRB region configurations would encode highly variable Mhc-DRB genes that are frequently exchanged by recombination. This has been documented for common marmosets, a New World monkey from the neotropical rain forests (32). In addition, the chicken has been reported to possess a "minimal essential Mhc" with two class II genes (43). These examples illustrate that evolutionary strategies for a functional Mhc-DR region are different between distinct species. It is remarkable that even within primate species different approaches are observed.



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  		FIGURE 2. Organization of the Mhc-DRB region configurations in humans and rhesus macaques. Shared loci/lineages are indicated by identical colors. In the case of HLA-DRB region configurations, the order of genes was determined by genomic mapping (17 ). In the case of the rhesus macaque, neither the exact order of genes nor whether the lineages represent different loci is known.

 
Furthermore, the present results have importance for the application of rhesus macaques in biomedical research, given that these primates are model species in many types of immunological experiments. This communication clearly illustrates the complexity of the Mamu-DR region, knowledge of which is important to facilitate the proper interpretation of the results of diverse experiments. MhcMamu typing is, for instance, needed to control the degree of MHC matching in transplantation studies and to determine restriction elements in vaccine and disease studies. Therefore, it is essential that concerted efforts will be made to obtain a comprehensive characterization of the entire Mamu region including classical and nonclassical Mhc genes.


   Acknowledgments
 
We thank Henk van Westbroek for preparation of the figure, Donna Devine for editing the manuscript, and Dr. Ilias Doxiadis for helpful discussion.


   Footnotes
 
1 This work was in part supported by Training and Mobility of Researchers Grant ERBFMGECT950024. Back

2 Address correspondence and reprint requests to Dr. G. G. M. Doxiadis, Department of Immunobiology, Biomedical Primate Research Centre, Lange Kleiweg 139, P.O. Box 3306, 2280 GH Rijswijk, The Netherlands. E-mail address: Back

3 Abbreviations used in this paper: BPRC, Biomedical Primate Research Centre; DGGE, denaturing gradient gel electrophoresis; SSP, sequence-specific primers; SSO, sequence-specific oligotyping; tr, triplet. Back

Received for publication October 6, 1999. Accepted for publication January 13, 2000.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References
 

  1. Heeney, J. L., C. van Els, P. de Vries, P. ten Haaft, N. Otting, W. Koornstra, J. Boes, R. Dubbes, H. Niphuis, M. Dings, et al 1994. Major histocompatibility complex class I-associated vaccine protection from simian immunodeficiency virus-infected peripheral blood cells. J. Exp. Med. 180:769.[Abstract/Free Full Text]
  2. Vogel, T. U., D. T. Evans, J. A. Urvater, D. H. O’Connor, A. L. Hughes, D. I. Watkins. 1999. Major histocompatibility complex class I genes in primates: co-evolution with pathogens. Immunol. Rev. 167:327.[Medline]
  3. Baskin, G. B., R. E. Bontrop, H. Niphuis, R. Noort, J. Rice, J. L. Heeney. 1997. Correlation of major histocompatibility complex with opportunistic infections in simian immunodeficiency virus-infected rhesus monkeys. Lab. Invest. 77:305.[Medline]
  4. Slierendregt, B. L., M. Hall, B. t Hart, N. Otting, J. Anholts, W. Verduin, F. Claas, M. Jonker, J. S. Lanchbury, R. E. Bontrop. 1995. Identification of an Mhc-DPB1 allele involved in susceptibility to experimental autoimmune encephalomyelitis in rhesus macaques. Int. Immunol. 7:1671.[Abstract/Free Full Text]
  5. Meinl, E., B. A. t Hart, R. E. Bontrop, R. M. Hoch, A. Iglesias, R. de Waal Malefyt, H. Fickenscher, I. Muller-Fleckenstein, B. Fleckenstein, H. Wekerle, et al 1995. Activation of a myelin basic protein-specific human T cell clone by antigen-presenting cells from rhesus monkeys. Int. Immunol. 7:1489.[Abstract/Free Full Text]
  6. Bakker, N. P., M. G. van Erck, N. Otting, N. M. Lardy, R. C. Noort, B. A. t’Hart, M. Jonker, R. E. Bontrop. 1992. Resistance to collagen-induced arthritis in a nonhuman primate species maps to the major histocompatibility complex class I region. J. Exp. Med. 175:933.[Abstract/Free Full Text]
  7. Bontrop, R. E., N. Otting, H. Niphuis, R. Noort, V. Teeuwsen, J. L. Heeney. 1996. The role of major histocompatibility complex polymorphisms on SIV infection in rhesus macaques. Immunol. Lett. 51:35.[Medline]
  8. Borleffs, J. C., R. L. Marquet, Z. de By-Aghai, W. van Vreeswijk, P. Neuhaus, H. Balner. 1982. Kidney transplantation in rhesus monkeys: matching for D/DR antigens, pretransplant blood transfusions, and immunological monitoring before transplantation. Transplantation 33:285.[Medline]
  9. Kirk, A. D., D. M. Harlan, N. N. Armstrong, T. A. Davis, Y. Dong, G. S. Gray, X. Hong, D. Thomas, Jr J. H. Fechner, S. J. Knechtle. 1997. CTLA4-Ig and anti-CD40 ligand prevent renal allograft rejection in primates. Proc. Natl. Acad. Sci. USA 94:8789.[Abstract/Free Full Text]
  10. Opelz, G.. 1988. Importance of HLA antigen splits for kidney transplant matching. Lancet 2:61.[Medline]
  11. Bodmer, J. G., S. G. Marsh, E. D. Albert, W. F. Bodmer, R. E. Bontrop, B. Dupont, H. A. Erlich, J. A. Hansen, B. Mach, W. R. Mayr, et al 1999. Nomenclature for factors of the HLA system, 1998. Eur J. Immunogenet. 26:81.[Medline]
  12. Klein, J., Y. Satta, N. Takahata, C. O’hUigin. 1993. Trans-specific Mhc polymorphism and the origin of species in primates. J. Med. Primatol. 22:57.[Medline]
  13. Figueroa, F., C. O’hUigin, H. Tichy, J. Klein. 1994. The origin of the primate Mhc-DRB genes and allelic lineages as deduced from the study of prosimians. J. Immunol. 152:4455.[Abstract]
  14. Gyllensten, U., M. Sundvall, I. Ezcurra, H. A. Erlich. 1991. Genetic diversity at class II DRB loci of the primate MHC. J. Immunol. 146:4368.[Abstract]
  15. Bontrop, R. E., N. Otting, B. L. Slierendregt, J. S. Lanchbury. 1995. Evolution of major histocompatibility complex polymorphisms and T-cell receptor diversity in primates. Immunol. Rev. 143:33.[Medline]
  16. Klein, J.. 1987. Origin of major histocompatibility complex polymorphism: the trans-species hypothesis. Hum. Immunol. 19:155.[Medline]
  17. Bontrop, R. E., N. Otting, N. G. de Groot, G. G. Doxiadis. 1999. Major histocompatibility complex class II polymorphisms in primates. Immunol. Rev. 167:339.[Medline]
  18. Geluk, A., D. G. Elferink, B. L. Slierendregt, K. E. van Meijgaarden, R. R. de Vries, T. H. Ottenhoff, R. E. Bontrop. 1993. Evolutionary conservation of major histocompatibility complex-DR/peptide/T cell interactions in primates. J. Exp. Med. 177:979.[Abstract/Free Full Text]
  19. Böhme, J., M. Andersson, G. Andersson, E. Moller, P. A. Peterson, L. Rask. 1985. HLA-DR ß genes vary in number between different DR specificities, whereas the number of DQ ß genes is constant. J. Immunol. 135:2149.[Abstract]
  20. Bontrop, R. E., D. G. Elferink, N. Otting, M. Jonker, R. R. de Vries. 1990. Major histocompatibility complex class II-restricted antigen presentation across a species barrier: conservation of restriction determinants in evolution. J. Exp. Med. 172:53.[Abstract/Free Full Text]
  21. Brändle, U., H. Ono, V. Vincek, D. Klein, M. Golubic, B. Grahovac, J. Klein. 1992. Trans-species evolution of Mhc-DRB haplotype polymorphism in primates: organization of DRB genes in the chimpanzee. Immunogenetics 36:39.[Medline]
  22. Slierendregt, B. L., R. E. Bontrop. 1994. Current knowledge on the major histocompatibility complex class II region in non-human primates. Eur. J. Immunogenet. 21:391.[Medline]
  23. Schönbach, C., V. Vincek, W. E. Mayer, M. Golubic, C. O’hUigin, J. Klein. 1993. Multiplication of Mhc-DRB5 loci in the orangutan: implications for the evolution of DRB haplotypes. Mamm. Genome 4:159.[Medline]
  24. Kenter, M., N. Otting, M. de Weers, J. Anholts, C. Reiter, M. Jonker, R. E. Bontrop. 1993. Mhc-DRB and -DQA1 nucleotide sequences of three lowland gorillas: implications for the evolution of primate Mhc class II haplotypes. Hum. Immunol. 36:205.[Medline]
  25. Slierendregt, B. L., N. Otting, N. van Besouw, M. Jonker, R. E. Bontrop. 1994. Expansion and contraction of rhesus macaque DRB regions by duplication and deletion. J. Immunol. 152:2298.[Abstract]
  26. Balner, H., J. J. Van Rood. 1971. Transplantation antigens in rhesus monkeys. Nature 232:121.
  27. Knapp, L. A., L. F. Cadavid, M. E. Eberle, S. J. Knechtle, R. E. Bontrop, D. I. Watkins. 1997. Identification of new Mamu-DRB alleles using DGGE and direct sequencing. Immunogenetics 45:171.[Medline]
  28. Slierendregt, B. L., J. T. van Noort, R. M. Bakas, N. Otting, M. Jonker, R. E. Bontrop. 1992. Evolutionary stability of transspecies major histocompatibility complex class II DRB lineages in humans and rhesus monkeys. Hum. Immunol. 35:29.[Medline]
  29. Lekutis, C., J. W. Shiver, M. A. Liu, N. L. Letvin. 1997. HIV-1 env DNA vaccine administered to rhesus monkeys elicits MHC class II-restricted CD4+ T helper cells that secrete IFN-{gamma} and TNF-{alpha}. J. Immunol. 158:4471.[Abstract]
  30. Paz-Artal, E., A. Corell, P. Varela, J. Martinez-Laso, E. Gomez-Casado, V. M. Fernandez-Soria, M. A. Moreno, A. Arnaiz-Villena. 1996. Primate DRB6 gene expression and evolution: a study in Macaca mulatta and Cercopithecus aethiops. Tissue Antigens 47:222.[Medline]
  31. Kenter, M., N. Otting, J. Anholts, M. Jonker, R. Schipper, R. E. Bontrop. 1992. Mhc-DRB diversity of the chimpanzee (Pan troglodytes). Immunogenetics 37:1.[Medline]
  32. Antunes, S. G., N. G. de Groot, H. Brok, G. Doxiadis, A. A. Menezes, N. Otting, R. E. Bontrop. 1998. The common marmoset: a new world primate species with limited Mhc class II variability. Proc. Natl. Acad. Sci. USA 95:11745.[Abstract/Free Full Text]
  33. Slierendregt, B. L., N. Otting, M. Jonker, R. E. Bontrop. 1994. Gel electrophoretic analysis of rhesus macaque major histocompatibility complex class II DR molecules. Hum. Immunol. 40:33.[Medline]
  34. Lekutis, C., N. L. Letvin. 1997. HIV-1 envelope-specific CD4+ T helper cells from simian/human immunodeficiency virus-infected rhesus monkeys recognize epitopes restricted by MHC class II DRB1*0406 and DRB*W201 molecules. J. Immunol. 159:2049.[Abstract]
  35. Lobashevsky, A. L., P. X. Wang, J. F. George, J. Contreras, J. Townsend, J. M. Thomas. 1998. DR non-B1 mismatches influence allogeneic MLR-induced TH1- or TH2-like cytokine responses in rhesus monkeys. Hum. Immunol. 59:363.[Medline]
  36. Mayer, W. E., C. O’hUigin, J. Klein. 1993. Resolution of the HLA-DRB6 puzzle: a case of grafting a de novo-generated exon on an existing gene. Proc. Natl. Acad. Sci. USA 90:10720.[Abstract/Free Full Text]
  37. Fernandez-Soria, V. M., P. Morales, M. J. Castro, B. Suarez, M. J. Recio, M. A. Moreno, E. Paz-Artal, A. Arnaiz-Villena. 1998. Transcription and weak expression of HLA-DRB6: a gene with anomalies in exon 1 and other regions. Immunogenetics 48:16.[Medline]
  38. Moreno-Pelayo, M. A., V. M. Fernandez-Soria, E. Paz-Artal, L. p. S. Ferre, M. Rosal, P. Morales, A. Arnaiz-Villena. 1999. Complete cDNA sequences of the DRB6 gene from humans and chimpanzees: a possible model of a stop codon readingthrough mechanism in primates. Immunogenetics 49:843.[Medline]
  39. Klein, J., C. O’hUigin. 1995. Class II B Mhc motifs in an evolutionary perspective. Immunol. Rev. 143:89.[Medline]
  40. Lobashevsky, A., J. P. Smith, J. Kasten-Jolly, H. Horton, L. Knapp, R. E. Bontrop, D. Watkins, J. Thomas. 1999. Identification of DRB alleles in rhesus monkeys using polymerase chain reaction-sequence-specific primers (PCR-SSP) amplification. Tissue Antigens 54:254.[Medline]
  41. Krings, M., A. Stone, R. W. Schmitz, H. Krainitzki, M. Stoneking, S. Pääbo. 1997. Neandertal DNA sequences and the origin of modern humans. Cell 90:19.[Medline]
  42. Hayasaka, K., K. Fujii, S. Horai. 1996. Molecular phylogeny of macaques: implications of nucleotide sequences from an 896-base pair region of mitochondrial DNA. Mol. Biol. Evol. 13:1044.[Abstract]
  43. Kaufman, J., J. Jacob, I. Shaw, B. Walker, S. Milne, S. Beck, J. Salomonsen. 1999. Gene organization determines evolution of function in the chicken MHC. Immunol. Rev. 167:101.[Medline]



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