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The Journal of Immunology, 1999, 163: 4091-4094.
Copyright © 1999 by The American Association of Immunologists

CUTTING EDGE

Cutting Edge: NKT Cell Development Is Selectively Impaired in Fyn- Deficient Mice

Gérard Eberl1, Bente Lowin-Kropf and H. Robson MacDonald

Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Epalinges, Switzerland


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results and Discussion
 References
 
Most NK1.1+ T (NKT) cells express a biased TCR{alpha}ß repertoire that is positively selected by the monomorphic MHC class I-like molecule CD1d. The development of CD1d-dependent NKT cells is thymus dependent but, in contrast to conventional T cells, requires positive selection by cells of hemopoietic origin. Here, we show that the Src protein tyrosine kinase Fyn is required for development of CD1d-dependent NKT cells but not for the development of conventional T cells. In contrast, another Src kinase, Lck, is required for the development of both NKT and T cells. Impaired NKT cell development in Fyn-deficient mice cannot be rescued by transgenic expression of CD8, which is believed to increase the avidity of CD1d recognition by NKT cells. Taken together, our data reveal a selective and nonredundant role for Fyn in NKT cell development.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results and Discussion
 References
 
Natural killer T (NKT) cells express a TCR{alpha}ß as well as phenotypic markers common to the NK cell lineage, including NK1.1 and IL-2Rß (CD122) (1, 2). Two types of NKT cells can be distinguished on the basis of their requirement for the monomorphic MHC class I-like molecule CD1d during development (3). The majority of NKT cells are CD1d dependent (4, 5, 6, 7), express a TCR repertoire biased to V{alpha}14 (8) and Vß8.2 (9, 10, 11), and segregate preferentially in thymus and liver (3). A second type of NKT cells is CD1d independent, expresses a diverse TCR repertoire, and is found mainly in spleen and bone marrow (3). A significant proportion of CD1d-independent NKT cells develop in the absence of the thymus, suggesting that the two types of NKT cells may belong to distinct cell lineages.

Protein tyrosine kinases of the Src family, including Fyn (p59fyn) and Lck (p56lck), are important components of proximal TCR/CD3 signaling (12). Fyn noncovalently associates with CD3{zeta}, but is not required for normal development of thymocytes and peripheral T cells (13, 14). In contrast, Lck associates with the cytoplasmic domains of CD4 and CD8{alpha} and is critical for both pre-TCR (15) and TCR (12) signaling. Indeed, Lck-deficient mice show a dramatic reduction in immature CD4+CD8+ thymocytes, no detectable single positive thymocytes, and a low number of peripheral T cells (16) .

While the signaling properties of Fyn and Lck have been extensively studied in conventional T cells, their role in NKT cell development and function is not clear. Interestingly, it has been reported that NKT cells do not develop in Fyn- and Lck-double deficient mice (17). Here, we tested whether Fyn and Lck, individually, are required for normal NKT cell development. Surprisingly, we find that the number of CD1d-dependent NKT cells is severely reduced in Fyn-deficient mice, whereas CD1d-independent NKT cells and conventional T cells are virtually unaffected. In Lck-deficient mice, only low numbers of both NKT and T cells can be found. These results demonstrate that Fyn plays a selective and nonredundant role in NKT cell development.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results and Discussion
 References
 
Mice

C57BL/6, Lck-deficient (Lck-/-), and CD8{alpha}ß-transgenic mice were purchased from Harlan/Netherlands (Zeist, The Netherlands), The Jackson Laboratory (Bar Harbor, ME), and Taconic (Germantown, NY), respectively. Fyn-deficient (Fyn-/-) mice were provided by Dr. Roger Perlmutter (Merck Research Laboratories, Rahway, NJ). All mutant mice had been back-crossed to C57BL/6 mice for several generations. CD8{alpha}ß-transgenic Fyn-/- or Lck-/- mice were derived at the Ludwig Institute from F2 intercrosses between CD8{alpha}ß-transgenic and Fyn-/- or Lck-/- mice or back-crosses between (CD8{alpha}ß-transgenic Fyn-/- or Lck-/-) F1 mice and Fyn-/- or Lck-/- mice. fyn typing was performed by PCR as described elsewhere (13), whereas Lck-/- mice were identified based on the severely decreased number of T cells in PBL as a consequence of Lck deficiency (16). All mice were used at 8–12 wk of age.

Cell preparation

Single-cell suspensions were prepared from liver, spleen, thymus, and bone marrow. Total liver cells were resuspended in a 40% isotonic Percoll solution (Pharmacia, Uppsala, Sweden) and underlaid with an 80% isotonic Percoll solution. Centrifugation for 20 min at 2000 rpm isolated the mononuclear cells at the 40–80% interface. The cells were washed twice with PBS containing 2% FCS. Spleen cells and bone marrow (femur, tibia) cells were resuspended in DMEM medium supplemented with 5% FCS and 1% HEPES and loaded onto 10-ml nylon wool columns that were preincubated overnight at 37°C with supplemented medium. The columns were incubated 45 min at 37°C and the cells, purified of B cells and monocytes, were harvested by washing the columns with 20 ml of supplemented medium. Thymocytes were resuspended in PBS containing 2% FCS together with a 1:10 dilution of B2A2 (anti-heat-stable Ag) hybridoma culture supernatants. After an incubation of 45 min at 4°C, the cells were washed and incubated for another 45 min at 37°C with an appropriate dilution of rabbit complement. The live mature (heat stable Ag-) thymocytes were isolated and washed twice.

Flow cytometry

A maximum of 1 x 106 cells were preincubated with 50 µl of 2.4G2 culture supernatant to block Fc{gamma} receptors. Cells were washed and incubated with the indicated mAb conjugates for 40 min in a total volume of 100 µl of PBS containing 2% FCS. Cells were washed and, if required, incubated with streptavidin conjugates for 20 min. After a further wash, cells were resuspended in PBS containing 2% FCS and analyzed on a FACScan flow cytometer (Becton Dickinson, San Jose, CA) for 3-color stainings or on a FACScalibur flow cytometer (Becton Dickinson) for 4-color stainings.

Antibodies

The following mAbs were purchased from PharMingen (San Diego, CA): FITC-, APC-, or biotin-conjugated anti-TCRß (H57-597), PE-conjugated anti-NK1.1 (PK136), FITC- or CyChrome-conjugated anti-CD4 (H129.19), and CyChrome-conjugated anti-CD8{alpha} (53-6.7). APC-conjugated streptavidin was purchased from Molecular Probes Europe (Leiden, The Netherlands). FITC-conjugated TCR Vß8.2 (F23.2) was prepared at the Ludwig Institute.


   Results and Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
References
 
We first determined the proportions of NKT (TCR{alpha}ß+ NK1.1+) and conventional T (TCR{alpha}ß+ NK1.1-) cells in several organs of C57BL/6 (wild-type), Fyn-deficient (Fyn-/-), and Lck-deficient (Lck-/-) mice. As reported previously (13, 14), the proportion of conventional T cells is similar in wild-type and Fyn-/- mice (Fig. 1GoA and data not shown). Surprisingly, the proportion of NKT cells is severely decreased in Fyn-/- mice, in particular in thymus and liver, and to a lesser extent in spleen and bone marrow (Fig. 1Go, A and C). The decrease of NKT cells in Fyn-/- mice is not the consequence of a lack of NK1.1 expression, because T cells found in Fyn-/- mice do not express a biased TCR repertoire (data not shown) as is the case for NKT cells in wild-type mice (9, 10, 11). In Lck-/- mice, the development of both NKT and conventional T cells is severely impaired (16, 17) (Fig. 1Go, A and C). These data indicate that Fyn plays a nonredundant role in NKT cell development, whereas Lck can apparently substitute for Fyn in T cell development (17, 18) .



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  		FIGURE 1. Impaired development of CD1d-dependent NKT cells in Fyn- or Lck-deficient mice. Thymus, liver, spleen, and bone marrow cells isolated from C57BL/6, Fyn-, or Lck-deficient mice were stained for TCR{alpha}ß, NK1.1, CD4, and CD8. A, Numbers indicate the proportion of NKT and T cells in liver. The experiment shown is representative of three independent experiments. B, Expression of CD4 and CD8 by liver NKT cells as gated in A. Numbers indicate the proportion of CD4+ or CD8+ NKT cells. The experiment shown is representative of three independent experiments. C, Absolute numbers of total NKT cells, CD4+ NKT cells, and CD8+ NKT cells in different organs of C57BL/6 ({blacksquare}), Fyn-deficient (), and Lck-deficient () mice. Data are the mean ± SD of three to five independent experiments.

 
Three NKT cell subsets can be defined on the basis of CD4/CD8 coreceptor expression, namely CD4+, CD4-CD8- (DN), and CD8+ NKT cells (1, 2). We have shown recently that most CD4+ NKT cells are CD1d dependent, whereas a significant proportion of DN NKT cells and most CD8+ NKT cells develop in the absence of CD1d (3). Moreover, CD1d-dependent NKT cells, and in particular CD4+ NKT cells, express a biased TCR repertoire, including a preferential usage of the Vß8.2 chain (3). In wild-type animals, a majority (~70%) of liver and thymus NKT cells express CD4 and very few express CD8 (~2%), whereas higher proportions of spleen and bone marrow NKT cells express CD8 (20–25%) or are DN (60–65%) (Fig. 1Go, B and C) (3). Interestingly, the subset composition of NKT cells is profoundly altered in Fyn-/- mice (Fig. 1Go, B and C). Indeed, the absolute number of CD4+ NKT cells is reduced ~20-fold in thymus and liver and 2- to 6-fold in spleen and bone marrow of Fyn-/- mice, whereas the number of CD8+ NKT cells is only marginally affected. Moreover, the overall proportion of Vß8.2+ NKT cells is significantly reduced in Fyn-/- mice (Fig. 2Go), even though residual CD4+ NKT cells still express a fully Vß8.2-biased TCR repertoire (Fig. 2GoB). Because Fyn-/- mice express normal levels of CD1d (data not shown), it is excluded that the absence of Fyn affects the development of CD1d-dependent NKT cells indirectly by regulating expression of CD1d. Together, these results show that the absence of Fyn selectively and severely impairs the development of CD1d-dependent (CD4+) NKT cells, but only marginally affects the development of CD1d-independent (CD8+) NKT cells. In Lck-/- mice, the few remaining NKT cells, found essentially in spleen and bone marrow, express CD8 (~40%) or are DN (50–60%) (Fig. 1Go, B and C) and do not express a biased TCR repertoire (Fig. 2Go).



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  		FIGURE 2. The TCR Vß repertoire of NKT cells in Fyn- or Lck-deficient mice. Thymus, liver, spleen, and bone marrow cells isolated from C57BL/6, Fyn-, or Lck-deficient mice were stained for TCR{alpha}ß, NK1.1, CD4, or CD8, and Vß8.2. A, Vß8.2 expression by liver NKT cells. Numbers indicate the proportion of Vß8.2+ cells among total NKT cells. The experiment shown is representative of three independent experiments. B, Percentage of Vß8.2+ cells among total NKT cells, CD4+ NKT cells, and CD8+ NKT cells in different organs of C57BL/6 ({blacksquare}), Fyn-deficient (), and Lck-deficient () mice. Numbers are the mean ± SD of three to five independent experiments. Low numbers of thymus CD8+ NKT cells and of CD4+ and CD8+ NKT cells in all organs of Lck-deficient mice did not permit an accurate estimation of Vß8.2 expression.

 
T cells from Fyn-/- mice have been shown in several instances to be hyporesponsive to Ag-, mitogen-, or Ab-mediated activation. Indeed, whereas peripheral T cells isolated from Fyn-/- mice respond normally to TCR-mediated activation, single positive (CD4+ or CD8+) thymocytes are refractile to stimulation (13, 14). Moreover, Utting et al. reported that single positive thymocytes and lymph node T cells isolated from Fyn-/- TCR-transgenic mice are hyporesponsive to stimulation by low-affinity or low-density cognate ligands (19). Finally, T cell lymphoproliferation and autoimmunity in lpr/lpr mice are suppressed in the absence of Fyn (20, 21). Taken together, these observations suggest that the absence of Fyn increases the avidity threshold required for T cell activation via the TCR. Therefore, it is reasonable to propose that Fyn may also increase the avidity threshold required for the development of CD1d-dependent NKT cells. As a consequence, NKT cell precursors expressing the semiinvariant TCR would fail to undergo positive selection in the absence of Fyn. Interestingly, Bendelac et al. have reported that a CD8{alpha}ß-transgene, expressed under the control of the CD2 promoter, prevents the development of CD1d-dependent NKT cells (defined as Vß8.2-biased CD44high thymocytes) (22). Therefore, it was suggested that CD8 expression is incompatible with the normal development of CD1d-dependent NKT cells, because CD8 interaction with CD1d (23) would lead to increased avidity of the semiinvariant TCR on NKT cell precursors for CD1d, ultimately resulting in negative selection (22). According to such a model, the absence of Fyn might be compensated by the presence of CD8 during development, leading to the appearance of a novel subset of CD8+ CD1d-dependent NKT cells in Fyn-/- mice. However, the absolute number of CD8+ NKT cells is not increased in Fyn-/- mice as compared with wild-type mice (Fig. 1GoC), and these cells do not express a Vß8.2-biased TCR repertoire in the absence of Fyn (Fig. 2GoB), as would be expected for CD1d-dependent NKT cells (3). Moreover, forced overexpression of a CD8{alpha}ß transgene in Fyn-/- mice does not rescue development of CD1d-dependent NKT cells (Fig. 3Go), and even inhibits development of the small number of CD1d-dependent NKT cells usually present in Fyn-/- mice (Fig. 3Go). Taken together, these data imply that physiological (or even elevated) levels of CD8 expression on NKT cells or their precursors does not set an avidity threshold appropriate for the development of CD1d-dependent NKT cells in the absence of Fyn. Clearly, other scenarios in which the expression of CD8 on NKT cell precursors may be qualitatively incompatible with the development of CD1d-dependent NKT cells remain to be tested.



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  		FIGURE 3. Failure to rescue CD1d-dependent NKT cell development in Fyn-deficient, CD8{alpha}ß-transgenic mice. Liver cells were isolated from wild-type, Fyn-deficient, and/or CD8{alpha}ß-transgenic littermate mice, and stained for TCR{alpha}ß, NK1.1, and Vß8.2. Upper panels indicate the proportion of total NKT or T cells in liver, and lower panels the proportion of Vß8.2+ cells among total liver NKT cells. Data are the mean ± SD of three independent experiments.

 
In addition to TCR-mediated signaling, Fyn is involved in signaling mediated by integrins, Thy-1, CD2, and IL receptors. It has been shown recently that ligation of ß1-integrins by components of the extracellular matrix induces association of Fyn with the integrin {alpha} subunit (24, 25) and with the focal adhesion kinase (26, 27), leading to activation of the Ras/Raf/mitogen-activated protein (MAP)/extracellular signal-related kinase (MEK)/MAP pathway and regulation of cell cycle progression. Nevertheless, NKT cells develop normally in mice expressing dominant negative mutants of Ras and/or MEK (28). Other surface receptors, including Thy-1 (29), the adhesion molecule CD2 (or LFA-2) (30, 31), and the IL receptors IL-2R/IL-15Rß (32, 33), IL-3R (34), IL-5R (35), and IL-7R (36, 37), activate and/or associate with Fyn upon binding of their cognate ligand. Several of these molecules have been shown to play a role in NKT cell development. Indeed, mice deficient for the common {gamma}-chain, a critical component of IL-2R, IL-4R, IL-7R, IL-9R, and IL-15R, have V{alpha}14+ (but NK1.1-) T cells in the thymus, but not in periphery, showing that development of CD1d-dependent NKT cells is incomplete (38). Moreover, IL-2R/IL-15Rß-/- mice have a marked defect in NKT cell development (39). Finally, even though the proportions of NKT cells are normal in IL-7-/- mice (but reduced 10-fold in terms of absolute numbers), they fail to produce cytokines upon stimulation (40). Therefore, the impaired development of CD1d-dependent NKT cells in Fyn-/- mice may reflect the requirement for TCR-independent pathways of activation mediated by adhesion molecules and/or IL receptors.

In conclusion, we have shown that the development of CD1d-dependent NKT cells is selectively impaired in Fyn-/- mice. Hence, Fyn may contribute to the maintenance of a signaling threshold that is critical for TCR-mediated positive selection of CD1d-dependent NKT cells, but not for selection of CD1d-independent NKT cells and conventional T cells. Alternatively (or in addition), Fyn may be involved in integrin, Thy-1, CD2, or IL receptor-mediated signaling that is critical for the development of CD1d-dependent NKT cells, but not for other subsets of T cells. Whatever the explanation, our data provide the first example of a signaling molecule that is required for NKT cell development, yet dispensable for development of both NK cells and conventional T cells.

Note added in proof.

Data complementery to those reported here have been obtained independently by Gadue et al. (41).


   Footnotes
 
1 Address correspondence and reprint requests to Dr. Gérard Eberl, Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Ch. des Boveresses 155, 1066 Epalinges, Switzerland. E-mail address: Back

Received for publication July 26, 1999. Accepted for publication August 9, 1999.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References
 

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J. A. Williams, J. M. Lumsden, X. Yu, L. Feigenbaum, J. Zhang, S. M. Steinberg, and R. J. Hodes
Regulation of Thymic NKT Cell Development by the B7-CD28 Costimulatory Pathway
J. Immunol., July 15, 2008; 181(2): 907 - 917.
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M. Felices and L. J. Berg
The Tec Kinases Itk and Rlk Regulate NKT Cell Maturation, Cytokine Production, and Survival
J. Immunol., March 1, 2008; 180(5): 3007 - 3018.
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 Proc. Natl. Acad. Sci. USAHome page
A. Veillette, S. Zhang, X. Shi, Z. Dong, D. Davidson, and M.-C. Zhong
SAP expression in T cells, not in B cells, is required for humoral immunity
PNAS, January 29, 2008; 105(4): 1273 - 1278.
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J. D. Wesley, M. S. Tessmer, C. Paget, F. Trottein, and L. Brossay
A Y Chromosome-Linked Factor Impairs NK T Development
J. Immunol., September 15, 2007; 179(6): 3480 - 3487.
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E. Hager, A. Hawwari, J. L. Matsuda, M. S. Krangel, and L. Gapin
Multiple Constraints at the Level of TCR{alpha} Rearrangement Impact V{alpha}14i NKT Cell Development
J. Immunol., August 15, 2007; 179(4): 2228 - 2234.
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C. Loh, Y.-C. Cai, G. Bonventi, G. Lajoie, R. MacLeod, and J. E. Wither
Dissociation of the Genetic Loci Leading to B1a and NKT Cell Expansions from Autoantibody Production and Renal Disease in B6 Mice with an Introgressed New Zealand Black Chromosome 4 Interval
J. Immunol., February 1, 2007; 178(3): 1608 - 1617.
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M. A. Jordan, J. M. Fletcher, D. Pellicci, and A. G. Baxter
Slamf1, the NKT Cell Control Gene Nkt1
J. Immunol., February 1, 2007; 178(3): 1618 - 1627.
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Y. Kunisaki, Y. Tanaka, T. Sanui, A. Inayoshi, M. Noda, T. Nakayama, M. Harada, M. Taniguchi, T. Sasazuki, and Y. Fukui
DOCK2 Is Required in T Cell Precursors for Development of V{alpha}14 NK T Cells.
J. Immunol., April 15, 2006; 176(8): 4640 - 4645.
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N. B. Martin-Cofreces, D. Sancho, E. Fernandez, M. Vicente-Manzanares, M. Gordon-Alonso, M. C. Montoya, F. Michel, O. Acuto, B. Alarcon, and F. Sanchez-Madrid
Role of Fyn in the Rearrangement of Tubulin Cytoskeleton Induced through TCR
J. Immunol., April 1, 2006; 176(7): 4201 - 4207.
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K. Benlagha, D. G. Wei, J. Veiga, L. Teyton, and A. Bendelac
Characterization of the early stages of thymic NKT cell development
J. Exp. Med., August 15, 2005; 202(4): 485 - 492.
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D. G. Wei, H. Lee, S.-H. Park, L. Beaudoin, L. Teyton, A. Lehuen, and A. Bendelac
Expansion and long-range differentiation of the NKT cell lineage in mice expressing CD1d exclusively on cortical thymocytes
J. Exp. Med., July 18, 2005; 202(2): 239 - 248.
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M. J. D. Wagner, S. Hussain, M. Mehan, J. M. Verdi, and T. L. Delovitch
A Defect in Lineage Fate Decision during Fetal Thymic Invariant NKT Cell Development May Regulate Susceptibility to Type 1 Diabetes
J. Immunol., June 1, 2005; 174(11): 6764 - 6771.
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 Proc. Natl. Acad. Sci. USAHome page
J. S. Bezbradica, T. Hill, A. K. Stanic, L. Van Kaer, and S. Joyce
Commitment toward the natural T (iNKT) cell lineage occurs at the CD4+8+ stage of thymic ontogeny
PNAS, April 5, 2005; 102(14): 5114 - 5119.
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C. Borowski and A. Bendelac
Signaling for NKT cell development: the SAP-FynT connection
J. Exp. Med., March 21, 2005; 201(6): 833 - 836.
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B. Chung, A. Aoukaty, J. Dutz, C. Terhorst, and R. Tan
Cutting Edge: Signaling Lymphocytic Activation Molecule-Associated Protein Controls NKT Cell Functions
J. Immunol., March 15, 2005; 174(6): 3153 - 3157.
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B. Pasquier, L. Yin, M.-C. Fondaneche, F. Relouzat, C. Bloch-Queyrat, N. Lambert, A. Fischer, G. de Saint-Basile, and S. Latour
Defective NKT cell development in mice and humans lacking the adapter SAP, the X-linked lymphoproliferative syndrome gene product
J. Exp. Med., March 7, 2005; 201(5): 695 - 701.
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T. A. Baldwin, K. A. Hogquist, and S. C. Jameson
The Fourth Way? Harnessing Aggressive Tendencies in the Thymus
J. Immunol., December 1, 2004; 173(11): 6515 - 6520.
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 Proc. Natl. Acad. Sci. USAHome page
K. Sugie, M.-S. Jeon, and H. M. Grey
Activation of naive CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling
PNAS, October 12, 2004; 101(41): 14859 - 14864.
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P. Gadue, L. Yin, S. Jain, and P. L. Stein
Restoration of NK T Cell Development in fyn-Mutant Mice by a TCR Reveals a Requirement for Fyn During Early NK T Cell Ontogeny
J. Immunol., May 15, 2004; 172(10): 6093 - 6100.
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A. K. Stanic, J. S. Bezbradica, J.-J. Park, N. Matsuki, A. L. Mora, L. Van Kaer, M. R. Boothby, and S. Joyce
NF-{kappa}B Controls Cell Fate Specification, Survival, and Molecular Differentiation of Immunoregulatory Natural T Lymphocytes
J. Immunol., February 15, 2004; 172(4): 2265 - 2273.
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H. Xu, T. Chun, A. Colmone, H. Nguyen, and C.-R. Wang
Expression of CD1d Under the Control of a MHC Class Ia Promoter Skews the Development of NKT Cells, But Not CD8+ T Cells
J. Immunol., October 15, 2003; 171(8): 4105 - 4112.
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N. Matsuki, A. K. Stanic, M. E. Embers, L. Van Kaer, L. Morel, and S. Joyce
Genetic Dissection of V{alpha}14J{alpha}18 Natural T Cell Number and Function in Autoimmune-Prone Mice
J. Immunol., June 1, 2003; 170(11): 5429 - 5437.
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T. Chun, M. J. Page, L. Gapin, J. L. Matsuda, H. Xu, H. Nguyen, H.-S. Kang, A. K. Stanic, S. Joyce, W. A. Koltun, et al.
CD1d-expressing Dendritic Cells but Not Thymic Epithelial Cells Can Mediate Negative Selection of NKT Cells
J. Exp. Med., April 7, 2003; 197(7): 907 - 918.
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K. L. Williams, A. J. Zullo, M. H. Kaplan, R. R. Brutkiewicz, C. D. Deppmann, C. Vinson, and E. J. Taparowsky
BATF Transgenic Mice Reveal a Role for Activator Protein-1 in NKT Cell Development
J. Immunol., March 1, 2003; 170(5): 2417 - 2426.
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P. Gadue and P. L. Stein
NK T Cell Precursors Exhibit Differential Cytokine Regulation and Require Itk for Efficient Maturation
J. Immunol., September 1, 2002; 169(5): 2397 - 2406.
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 Int ImmunolHome page
N. Baur, G. Nerz, A. Nil, and K. Eichmann
Expression and selection of productively rearranged TCR{beta} VDJ genes are sequentially regulated by CD3 signaling in the development of NK1.1+ {alpha}{beta} T cells
Int. Immunol., August 1, 2001; 13(8): 1031 - 1042.
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 Am. J. Respir. Cell Mol. Bio.Home page
E. M. Kudlacz, C. J. Andresen, M. Salafia, C. A. Whitney, B. Naclerio, and P. S. Changelian
Genetic Ablation of the src Kinase p59fynT Exacerbates Pulmonary Inflammation in an Allergic Mouse Model
Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 469 - 474.
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E. Assarsson, T. Kambayashi, J. K. Sandberg, S. Hong, M. Taniguchi, L. Van Kaer, H.-G. Ljunggren, and B. J. Chambers
CD8+ T Cells Rapidly Acquire NK1.1 and NK Cell-Associated Molecules Upon Stimulation In Vitro and In Vivo
J. Immunol., October 1, 2000; 165(7): 3673 - 3679.
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C. Viret, O. Lantz, X. He, A. Bendelac, and C. A. Janeway Jr.
A NK1.1+ Thymocyte-Derived TCR {beta}-Chain Transgene Promotes Positive Selection of Thymic NK1.1+ {alpha}{beta} T Cells
J. Immunol., September 15, 2000; 165(6): 3004 - 3014.
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D. Elewaut, L. Brossay, S. M. Santee, O. V. Naidenko, N. Burdin, H. De Winter, J. Matsuda, C. F. Ware, H. Cheroutre, and M. Kronenberg
Membrane Lymphotoxin Is Required for the Development of Different Subpopulations of NK T Cells
J. Immunol., July 15, 2000; 165(2): 671 - 679.
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D. G. Pellicci, K. J.L. Hammond, A. P. Uldrich, A. G. Baxter, M. J. Smyth, and D. I. Godfrey
A Natural Killer T (NKT) Cell Developmental Pathway Involving a Thymus-dependent NK1.1-CD4+ CD1d-dependent Precursor Stage
J. Exp. Med., March 25, 2002; 195(7): 835 - 844.
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