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Department of Immunology, Paul-Ehrlich-Institute, Langen, Germany
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Abstract |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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Introduction |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Although AICD has been extensively studied over the past years, only a
few reports have analyzed the potential modulation of AICD by
coligation of other T cell surface molecules. For example, ligation of
CD28 has been demonstrated to inhibit subsequent apoptosis by TCR/CD3
ligation in murine and human T cells (9, 10). We have
reported that preligation of CD4 molecules on T cell clones with mAb or
gp120 of HIV significantly inhibits AICD (11). The
inhibition was associated with a diminished TCR-induced tyrosine
phosphorylation, reduced levels of CD3-inducible FasL mRNA and FasL
surface expression, and a block of TCR-stimulated production of IFN-
and IL-2.
In the present study, we obtained evidence for a biochemical link between CD4 engagement and negative regulation of TCR/CD3 stimulation and AICD. We identified the product of the pro- tooncogene Vav (12) and the Vav-associated multifunctional SH2 domain-containing leukocyte protein of 76 kDa (SLP-76; Refs. 13, 14, 15) as components of TCR/CD3 signaling cascade that are differentially phosphorylated upon TCR ligation in CD4-stimulated vs unstimulated T cell clones.
The three functional domains of SLP-76 mediate binding to
SH2-containing proteins, including Vav (acidic N terminus when
phosphorylated), to SH3-containing molecules such as Grb-2
(proline-rich central part), and tyrosine-phosphorylated molecules such
as SLAP-130 (=FYB) and pp62 (C-terminal SH2 domain) (15).
More recently, studies with SLP-deficient T cells and
SLP-76-/- mice revealed a profound role of this
adapter protein in T cell development and activation
(16, 17, 18). Thus, SLP-76 couples TCR-associated PTKs to
PLC-1-induced signaling cascades, and is absolutely required for
normal T cell development and function. Interestingly, the expression
of SLP-76 is restricted to T lymphocytes. In B cells, BLNK or SLP-65,
an adapter protein with a very similar overall structure, but a
relatively low homology to SLP-76, fulfills the same role in linking
the B cell receptor signals to PLC-1 activation (19, 20).
To our knowledge, a direct association of SLP-76 with the TCR/CD3/CD4-complex or the respective src-related kinases p56lck or p59fyn has not been demonstrated. We show that SLP-76 does not only interact with Grb-2 in a SH3-mediated fashion, but also directly, and selectively binds to SH3 domains of p56lck. With a peptide competition strategy, we map the binding site to a proline-rich stretch located between amino acids 185 and 194 of SLP-76, which is different from the sequence that has been shown to mediate Grb-2 binding. Most important, we are able to coprecipitate substantial amounts of p56lck with SLP-76 from lysates of untransfected Jurkat cells.
Our results suggest that upon ligation of CD4, crucial components of the TCR/CD3 signaling cascade (i.e., SLP-76 and Vav) are sequestered to the CD4-p56lck complex via SH3-mediated interactions. As a consequence, we observe a transient TCR/CD3 desensitization of anti-CD4-pretreated cells and provide a model to explain the reduced TCR-dependent cytokine production and AICD described before (11).
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Materials and Methods |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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The IL-2-dependent CD3+CD4+CD5+ T cell clone D894/25 has been described (3, 11). Clone cells were restimulated periodically with irradiated PBMC and EBV-transformed lymphoblastoid cell lines in the presence of PHA (0.5 µg/ml; Wellcome, Burgwedel, Germany). Three days after restimulation, the cells were washed extensively and further expanded in the presence of human rIL-2 (10 U/ml; EuroCetus, Frankfurt, Germany) for several days before use in the assays. Dead cells were removed by Ficoll gradient centrifugation when necessary. For biochemical analysis, we also used leukemic Jurkat cells (clone E6.1; American Type Culture Collection (ATCC), Manassas, VA) and PHA-stimulated peripheral blood T lymphocyte populations. To this end, PBMC were cultured with PHA (0.5 µg/ml) for 3 days and further expanded in rIL-2-containing medium. All cells were grown at 37°C in a humidified atmosphere with 6% CO2. Culture medium was RPMI 1640 with 10% (v/v) FBS (Biochrom, Berlin, Germany), antibiotics (penicillin at 100 U/ml and streptomycin at 100 µg/ml), L-glutamine (2 mM), and HEPES buffer solution (10 mM).
Antibodies and reagents
For stimulation of the TCR/CD3 complex, we used anti-CD3 mAb OKT3 (mouse IgG2a; Cilag, Sulzbach, Germany) or staphylococcal enterotoxin superantigens from Toxin Technologies (Sarasota, FL). Anti-CD3 mAb were cross-linked with rabbit anti-mouse IgG secondary Abs (Jackson ImmunoResearch, West Grove, PA) when indicated. For ligation or immunoprecipitation of CD4, mAb OKT4 (mouse IgG2b, hybridoma from ATCC) and 5F8 (mouse IgG1, generated in our laboratory) were used. Anti-CD5 mAb UCHT2 (provided by the Sixth International Leukocyte Typing Workshop) was utilized as a control in some experiments. The anti-SLP-76 sheep polyclonal antiserum and anti-pTyr mAb 4G10 were purchased from Upstate Biotechnology (Lake Placid, NY). Anti-c-Cbl (C-15, rabbit polyclonal antiserum), anti-Vav (C-12, rabbit polyclonal antiserum), and anti-Sam68 (7-1, mAb) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). The anti-p56lck antiserum was raised against a lck-specific peptide (residues 39–64) (22), and mAb 4/215 (IgG1) against the SH3lck fusion protein (this laboratory). Anti-GST mAb B11F8 (this laboratory) was used for far Western blotting with GST fusion proteins. For Western blotting with ECL detection (Amersham, Braunschweig, Germany), HRP-conjugated secondary reagents (anti-IgG) from Rockland (Gilbertsville, PA; rabbit anti-sheep) and Amersham (donkey anti-rabbit, rabbit anti-mouse) were used. The expression and purification of GST fusion proteins containing the SH2 and/or SH3 domains of p56lck and p59fyn(T) have been described elsewhere (21, 22).
T cell stimulation and preparation of cell lysates
To determine effects of CD4 ligation on TCR signals, 1.5 x 106 (total cell lysates) or 20–50 x 106 (precipitations) clone cells per sample were incubated in the absence or presence of anti-CD4 (or anti-CD5) mAb at 10 µg/ml for 1 h at 37°C. In other experiments, respective numbers of Jurkat cells were used without CD4 engagement. Before TCR stimulation, the cells were washed twice in RPMI with 2% FBS. A total of 50 µl of prewarmed Ab solution with mAb OKT3 at 10 µg/ml and cross-linking Abs at 1 µg/ml were added to the pellet, and incubation was performed at 37°C for the indicated intervals. Clone cells were also activated with SEA at 5 ng/ml. Stimulation was stopped by adding 1 ml of cold PBS, quick spin centrifugation, aspiration of the PBS/Ab supernatant, and immediate lysis in 30 µl of Brij96 (Sigma, Deisenhofen, Germany) or Nonidet P-40 (Fluka Chemie AG, Buchs, Switzerland) lysis buffer (1% (v/v) of detergent in 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, with protease and phosphatase inhibitors aprotinin (10 µg/ml), leupeptin (10 µg/ml), 1 mM PMSF, 1 mM sodium orthovanadate, 1 mM sodium pyrophosphate (all from Sigma), and 10 mM sodium fluoride (Fluka)). Lysates remained on ice for 15–30 min before centrifugation at 4°C and 14,000 rpm for 7 min. Supernatants were then transferred into fresh tubes for further analysis.
Immunoprecipitations, precipitations with fusion proteins, and Western blotting
For precipitation with fusion proteins or immunoprecipitation, supernatants were incubated for 90 min rotating at 4°C with 2–5 µg of the respective Ab or 20–50 µg of GST fusion protein. A total of 60 µl of a 50% slurry of protein A-Sepharose CL4B, protein G-Sepharose, or glutathione Sepharose 4B beads (Pharmacia, Piscataway, NJ) was added, and the samples were rotated for an additional 30 min. The beads were pelleted, washed thrice in cold lysis buffer, and boiled in sample buffer containing 2-ME. Total cell lysates boiled with an equal volume of sample buffer or precipitates were loaded onto SDS polyacrylamide gels. Separated proteins were transferred to nitrocellulose membranes (Hybond C-Extra; Amersham). Protein loading and efficiency of transfer were monitored with Ponceau S (Sigma). The blots were blocked with 5% BSA (Sigma) for 1 h, and proteins were analyzed with the indicated primary and secondary Abs or with fusion proteins, anti-GST mAb, and HRP-conjugated anti-mouse Ig, and ECL detection reagents.
Peptide competition assay
The peptides listed in Table I
were synthesized on an AMS 422 peptide synthesizer (Abimed, Langenfeld,
Germany). All peptides were dissolved in PBS and used at concentrations
specified in Results. For competition experiments, 20 µg
of the respective fusion protein on beads was incubated with peptide
for 10–15 min at 4°C with constant rotation. A total of 100 µl of
filtered cell lysates corresponding to 25–50 x
106 cells was then added, and incubation was
prolonged for 10 min. The beads were then washed extensively and
subjected to SDS-PAGE and Western blotting, as described. For ex vivo
peptide competition of the SLP-76/lck
interactions, filtered lysates of 400 x
106 Jurkat cells were incubated overnight,
rotating at 4°C with 2 mM of peptides, followed by SLP-76
immunoprecipitation and anti-lck immunoblot.
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Results |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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CD4+CD3+ T cells
(clone D894/25) were incubated for 1 h in the presence or absence
of anti-CD4 (OKT4) or anti-CD5 (UCHT2) mAbs before stimulation
with SEA for 2 or 5 min. As shown in Fig. 1A, a selective reduction of
the level of tyrosine phosphorylation of polypeptides with approximate
m.w. of 40–44, 48–50, 62–64, 76, 80, 95, and 145–150 kDa (arrows)
was observed in Nonidet P-40 lysates of CD4-treated cells. Preligation
of CD4 also inhibited SEA-induced cytokine production and AICD in
894/25 cells (11). Time-course experiments revealed that
CD4 ligation was most effective in reducing the subsequent
superantigen-induced phosphorylation when CD4 engagement was initiated
at least 30 min before the TCR stimulation (not shown). To identify
proteins that may be involved in the CD4-mediated down-regulation of
the SEA-induced TCR response, we first focused on the polypeptide of 76
kDa. Recently, the SH2 domain-containing leukocyte protein of 76 kDa
(SLP-76) has been described as an essential transducer of TCR signals
to IL-2 gene activation in concert with Vav, Grb-2, and ZAP-70
(23, 24, 25). We therefore reprobed the same blot shown in
Fig. 1A with an anti-SLP antiserum and visualized equal
amounts of SLP-76 protein loaded in each lane (Fig. 1B). The
overlay of the two films strongly suggested that the 76-kDa band in
fact represented SLP-76. Of note, CD5 ligation before TCR stimulation
did not alter TCR-initiated phosphorylation of the indicated proteins,
suggesting that the observed inhibition might be strictly dependent on
the activation of the CD4 molecule or the
CD4/p56lck complex.
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With the next set of experiments, the SEA-induced tyrosine
phosphorylation of SLP-76 and Vav was analyzed with or without
preceding engagement of CD4 by mAbs. Activation of these proteins
during TCR-stimulated cytokine production has been shown to be
associated with an increased tyrosine phosphorylation mediated by
ZAP-70 (23, 24, 25). We therefore immunoprecipitated SLP-76
and Vav, respectively, from unstimulated (-) or
superantigen-stimulated (+) clone cells that had been exposed or not to
anti-CD4 mAb OKT4 for 1 h and analyzed the tyrosine
phosphorylation of the blotted proteins with anti-pTyr mAb 4G10
(Fig. 2A). Both proteins were
tyrosine phosphorylated during a 3-min stimulation with SEA (see also
total cell lysates (TCL)), and the TCR-induced phosphorylation of both
proteins was almost completely blocked after exposure to anti-CD4
mAb before SEA stimulation. The protein content in precipitates of
untreated or CD4-treated cells was checked by reprobing the blot with
anti-Vav (B) or anti-SLP-76 (C)
Abs.
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From the experimental data shown so far, we hypothesized that Vav
and/or SLP-76 should be able to interact with both the
CD4/p56lck complex and the
TCR/CD3/p59fyn complex in a competitive manner.
Also, the respective interaction should be independent of tyrosine
phosphorylation. Given the structure of SLP-76 with three protein
interaction domains including the proline-rich central portion
(26, 27), we favored a potential SH3 association with
p56lck and p59fyn(T) in T
cells. Therefore, we investigated whether SLP-76 can be detected in
SH3-based precipitates from Nonidet P-40 lysates of T cells. For
experimental reasons, these studies were initially done with Jurkat
cells. However, similar results were obtained with clone cells and with
PHA-stimulated T cell lines. In these experiments, we detected a
phosphotyrosine-containing protein band in SH3 precipitates that
comigrated with immunoprecipitated SLP-76 and phosphorylated SLP-76 in
cell lysates from CD3-stimulated cells (Fig. 3A, arrow). As can also be
seen in A, we observed a preferential binding of SLP-76 to
isolated SH3 domains of p56lck as compared with
p59fyn(T). Again, we detected an increased
tyrosine phosphorylation of SLP-76 upon stimulation of Jurkat cells
with anti-CD3 mAb OKT3 (Fig. 3B, +). The presence of
equal amounts of protein was tested by reprobing the blot with
anti-SLP-76 antiserum (Fig. 3C). In addition, we were
able to verify a direct SH3-mediated association with
p56lck in a far Western approach utilizing the
GST-SH3lck fusion protein and an anti-GST mAb to detect
SH3-binding SLP-76 in TCL and anti-SLP-76 immunoprecipitates (Fig. 3D).
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SH3 binding is achieved by an interaction of proline-rich motifs
of the target protein with a specific binding pocket within the SH3
domain (28, 29). Although SH3 binding occurs independently
of tyrosine phosphorylation, it has been suggested that SH3-mediated
interactions provide a means to recruit substrates to the receptor/PTK
complexes. In fact, as shown in Fig. 4, a
rapid (on after 10–30 s) and transient (off after 10–15 min) tyrosine
phosphorylation of SLP-76 protein immunoprecipitated from lysates of
OKT3-stimulated Jurkat cells is seen (Fig. 4A).
GST-SH3lck fusion proteins precipitate phosphorylated and
nonphosphorylated SLP-76. The GST part of the fusion protein did not
yield any detectable protein (Fig. 4B). As has been reported
for other SH3-binding proteins, SLP-76 binding to the SH3 domain was
not significantly influenced by CD3-induced tyrosine phosphorylation.
Thus, SLP-76 protein levels were comparable in all
GST-SH3lck precipitates (Fig. 4B). Kinetics of
SLP-76 phosphorylation in SH3 precipitates was slightly different in
that a weak phosphorylation remained detectable even after 30 min of
OKT3 stimulation. Of note in this context, SLP-76 coprecipitated with a
number of yet unidentified tyrosine-phosphorylated proteins with
approximate m.w. of 42–44, 62–64, and 120 kDa under these
conditions.
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To further analyze the specificity of SLP-76 SH3 binding to Src
kinases, we compared protein levels precipitated from PHA-stimulated
PBMC with GST-SH3 fusion proteins of p56lck and
of p59fyn(T). As shown in Fig. 5, no SLP-76 protein was detected in
controls using protein A and protein G beads (c) or GST and
glutathione beads. GST-SH3lck precipitated SLP-76 more
efficiently than GST-SH3fyn, pointing to a preferential
association of SLP-76 to the CD4-associated PTK. Again, the amount of
SLP-76 was unchanged in precipitates from unstimulated (-) vs
OKT3-stimulated (+) cells.
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To identify potential docking regions of SLP-76 binding to SH3
domains of p56lck, we chose a peptide
competition strategy. To this end, a series of peptides was synthesized
according to the published SLP-76 and c-Cbl sequences (14, 30). SLP-76 peptides B-K contained proline residues
characteristic for putative SH3 binding sites (Table I
). In addition,
we used a control SLP-76 peptide (peptide A) without proline residues
and two peptides corresponding to potential SH3-binding regions within
c-Cbl (peptides L and M). When SH3 domains of
p56lck were preincubated as GST-fusion proteins
with the different peptides at a concentration of 1 mM, only peptides E
(QPPVPPQRPM, corresponding to amino acids 185–194 of SLP-76) and L
(LPPPPPPDRP, corresponding to amino acids 542–551 of c-Cbl)
efficiently competed for binding of SLP-76 protein from Jurkat cells
(Fig. 6A). Identical results
were obtained when clone cells or PHA-stimulated PBMC were used (not
shown). Interestingly, under these conditions, peptide E (of SLP-76)
also completely blocked SH3 binding to Sam68 (31, 32, 33, 34) and
c-Cbl, whereas peptide L markedly reduced the SH3/c-Cbl interaction,
but only mildly inhibited binding of Sam68 (Fig. 6A). This
result was confirmed by titration experiments, as shown for peptides E
and L in Fig. 6B. Peptide E inhibited precipitation of Sam68
and c-Cbl with SH3 domains of p56lck at a 4-fold
lower dose compared with peptide L. Of note in this context, peptide E
effectively competed off SLP-76 binding to SH3lck, but did
not significantly influence the association of SLP-76 with GST-Grb-2
fusion proteins. In addition, peptides E and L, but not peptide A,
covalently linked to activated Sepharose beads, in vitro bound
GST-SH3lck fusion protein, but not to GST alone (not
shown).
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Having established the association of SLP-76 and
p56lck in vitro, the next relevant
experiment was to show that the two proteins can be coprecipitated from
intact cells. Being aware of the difficulties of such experiments due
to the instablity of protein-protein complexes even under mild lysis
conditions, we chose the following strategy: Brij96 lysates from
50 x 106 unstimulated Jurkat cells were
subjected to immunoprecipitation with anti-lck mAb (Fig. 7A, lane 5) or
anti-lck antiserum (lane 6). For the
anti-SLP-76 immunoprecipitation (Fig. 7A, lane
7), we increased the cell number to 400 x
106. Protein G-bound immunocomplexes were
separated by SDS-PAGE on a 8.5% gel. Upon Western blotting of the
precipitated proteins, p56lck protein was
analyzed first with the anti-lck mAb (upper
panel) and reprobed with the polyclonal antiserum
(lower panel). As can be seen in Fig. 7, p56lck (and p60lck) could
be detected by this type of analysis with both the monoclonal and the
polyclonal anti-lck Abs not only in immunoprecipitates
formed by anti-lck reagents (lanes 5
and 6), but also in anti-SLP-76 immunocomplexes
(lane 7). This clearly established that the two
proteins, SLP-76 and p56lck, can be
coprecipitated as a fairly stable complex from unstimulated intact
cells. As shown in Fig. 7B, under similar conditions,
peptide E (lane 9), but not control peptide A
(lane 8), interfered with the coprecipitation of
p56lck (lane 5 or
7). Also, depletion of lysate from CD4/lck by
three rounds of anti-CD4 immunoprecipitation almost completely
reduced the amount of lck in the subsequent anti-SLP-76
immunoprecipitation.
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Discussion |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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We have previously observed that CD4 stimulation of cloned T cells with mAb or HIV-1 gp120 modified subsequent TCR signaling, resulting in a marked reduction of cytokine production and inhibition of AICD (11). This inhibition of TCR signaling was associated with reduced levels of tyrosine phosphorylation of a number of proteins detected in whole cell lysates (11). We now report that two PTK substrates implicated in TCR-mediated signal transduction, the product of the protooncogene vav (12, 43, 44, 45) and the associated SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) (14, 15, 16, 17, 18, 25, 26, 27), show this characteristic inhibition of TCR-inducible tyrosine phosphorylation in clone cells pretreated with anti-CD4 mAb. Our results demonstrate that CD4 ligation can prevent the formation of signal-transducing units around the TCR/CD3 complex leading to a desensitization of the Ag receptor.
Recently, Jabado and colleagues also pointed to a defective formation
of p21ras activity-regulating complexes,
including PLC-1, p120GAP, and other yet
unidentified proteins upon CD4 ligand binding (46). In
addition, Goldman et al. suggested that CD4 ligation may inhibit TCR
signaling by sequestering p56lck to the
actin-cytoskeleton (47). When they pretreated cells with
gp120 1–4 h before stimulation, TCR-directed PTK activation was
markedly reduced. Also, in gp120/anti-gp120-stimulated cells,
p56lck was found predominantly in association
with the cytoskeleton in the Nonidet P-40 detergent-insoluble
cytoskeletal, but not in the Nonidet P-40 detergent-soluble cytosolic
fractions (47).
With Vav and SLP-76, we link two other key players in TCR signal transduction to the CD4/p56lck complex. The multifunctional adapter protein SLP-76 was characterized as a molecule that is phosphorylated by ZAP-70 upon TCR stimulation and subsequently associates with SH2 domains of Vav via phosphotyrosyl-containing motifs in the N-terminal portion of SLP-76 (13, 23, 24, 25). The physical link between Vav and SLP-76 may also be differentially regulated by associated phosphatases including CD45 (48). In addition, a constitutive association of SLP-76 with Grb-2 SH3 domains has been reported, mediated by the proline-rich central part (14, 15). With its C-terminal SH2 domain, SLP-76 also reacts with phosphoproteins including SLAP-130 (=FYB) and pp62 (27, 49, 50).
Recent studies with SLP-76-deficient mice and SLP-76-deficient T cell
lines revealed a very profound role of the adapter protein in T cell
development and activation (16, 17, 18). It was shown that
SLP-76 couples TCR-associated PTKs to PLC-1-induced signaling
cascades and that it is absolutely required for normal T cell
development and function. Interestingly, the expression of SLP-76 is
restricted to hemopoietic cells of monocyte, granulocyte, and T
lymphocyte lineage (51), whereas in B cells, SLP-65 (or
BLNK), an adapter protein with a very similar overall domain structure
but fairly low homology, fulfills the same role in linking the B cell
receptor signals to PLC-1 activation (19, 20).
We show that besides the multiple interactions of SLP-76 that have been reported to date, the adapter protein also binds to SH3 domains of Src kinases, preferentially of p56lck. The amount of SLP-76 precipitated with SH3 domains of p56lck was comparable with the amount of protein precipitated with full-length Grb-2 fusion proteins (not shown). Using a peptide competition strategy, we mapped the putative binding region to a XPPXPPXXP motif corresponding to amino acids 185–194 of SLP-76. Interestingly, the SLP-76 peptide (QPPVPPQRPM) used for the competition experiments also completely blocked the SH3lck interaction with Sam68 and c-Cbl, indicating that in fact it tightly occupies the binding pocket of the SH3 domain. Also of note, in vitro SH3 binding to isolated domains of p59fyn was consistently weaker, indicating a preferential association with the SH3 domain of p56lck. Furthermore, peptide E effectively competed off SLP-76 binding to SH3lck, but did not significantly influence association with GST-Grb-2. Interestingly, according to the work by Motto et al. (15), the interaction of SLP-76 with either or both Grb-2 SH3 domains occurs via amino acids 225–244 of SLP-76, an area that does not contain an obvious SH3-binding motif.
Although a preferential binding of type 1 ligand motifs (R/KxxPxxP) to SH3 domains of Src kinases has been suggested, peptide E (and also peptide L) contains type II SH3-binding motifs (PxxPxR). However, the binding selectivity of Src-SH3 domains for type I or type II motifs seems to be far from exclusive. The reports by Yu et al. (29), Feng and colleagues (52), and Lim et al. (53) showed that the ligands share a common invariable PxxP core motif, and that peptide binding is mainly dependent on the critical salt bridging of the arginine residue of the peptide and the conserved aspartate at position 99 of the Src-family SH3 domain, with the residues in positions 3, 4, 6, and 7 of the peptide intercalating into the binding site and thus determining the binding orientation of the peptide. This was also confirmed by Morton and colleagues (54), who investigated ligand binding to the SH3 domain of Fyn. Based on the comparison of different peptides (including type I and type II) for SH3 binding, they suggested that SH3 domains bind to polyproline peptides in a promiscuous manner, although the ligand with the closest match to the class I consensus sequence bound with highest affinity and in the predicted orientation. Also, the NMR of the SH3 domain of lck (55) confirmed the previous suggestions that peptide ligands can bind in two different orientations to SH3 domains. This study also points to some unique features within the SH3 domain of Lck, which could help to explain the observed preference of SLP-76 for Lck compared with Fyn. In view of the specific regulation of SH3 binding by the critical arginine residue, it should also be mentioned that peptides D (aa 182–191) and E (aa 185 to 194) are overlapping peptides that share seven amino acids including the PxxP core. However, only the inhibitory peptide E contains an arginine residue in a position that determines the type II-binding motif PxxPxR.
Most important, we were able to demonstrate that p56lck can be coprecipitated in an immunocomplex formed by anti-SLP-76 Abs from unstimulated Jurkat cells, and that the coprecipitation can be abrogated by preincubation of the cell lysates with the competitor peptide E. This established that the phosphorylation-independent (SH3-mediated) association detected and analyzed in vitro is also operational in vivo in intact cells.
The SH3 domain-mediated interaction of SLP-76 with
p56lck may help to explain the observations
mentioned above (46, 47). Since we found a reduced
tyrosine phosphorylation of SLP-76 upon CD4 pretreatment, it seems
likely that CD4 ligation resulted in a spatial dissociation from its
kinase, ZAP-70. Thus, if p56lck is not available
for the phosphorylation of the 
-chains, ZAP-70 could not bind to the
TCR/CD3 complex to get activated. We are presently analyzing whether
the dissociation occurs due to sequestering
p56lck to the cytoskeleton, as has been
suggested by Goldman and colleagues (47), or by capping
mechanisms that have been described for other systems. In fact, the
influence of the CD4 proximity to the TCR/CD3 complex had been
correlated earlier to inhibitory or stimulatory signals provided by CD4
(56).
As a functional outcome of negative tuning of TCR responses by CD4 engagement, the selective activation of CD4-positive naive but not memory cells has been suggested as a consequence of MHC class II molecules on APC and CD4 ligation (57). In the context of thymocyte development, it was shown that CD4 engagement inhibited TCR expression and function in immature CD4+CD8+ thymocytes (58). The effects of other ligands of CD4, including envelope proteins of HIV and the recently cloned IL-16, seem to be manyfold and very much depending on the investigated system. For IL-16 as a natural ligand of CD4 (59, 60), a block of CD3-dependent lymphocyte activation and proliferation has been reported by Cruikshank and coworkers (61). In addition, IL-16 seems to play a role in the regulation of HIV-1 infection and/or replication (62, 63, 64). A protective effect of rIL-16 against AICD has also been described, although the underlying mechanism needs further studies (64). The preparation of IL-16 that we used in our previous study was ineffective in our system. Moreover, CD4 ligation in our hands affected CD95L but not CD95 expression (11), as has been suggested by others (64).
The multiple effects of cross-linking of CD4 by anti-CD4 mAb or gp120 of HIV on TCR-induced activation of resting vs preactivated cells have been extensively discussed in our previous report (11). They range from costimulation of resting T cells to proliferate (65, 66), priming of resting cells for AICD (67, 68, 69, 70) and direct induction of apoptosis (71, 72, 73, 74, 75, 76) to the inhibition of TCR-induced activation (46, 47, 77, 78, 79, 80), and AICD (11). Taken together, these studies suggest that the observed differences in the CD4/TCR cross-talk between resting and activated cells very much depend on the investigated T cell population. Overall, resting cells seem to be coactivated by CD4 engagement, whereas inhibitory effects of CD4 ligation have been mainly observed in preactivated populations. Although OKT4 does not bind to the gp120-binding region, it needs to be addressed in further studies whether the binding of different CD4 ligands or mAb to distinct functional epitopes within the CD4 molecule provokes different signals with regard to the inhibition of AICD (81).
In conclusion, SH3-mediated interactions of SLP-76 and associated
molecules with the CD4/p56lck complex play a
crucial role in negative signaling through the CD4 molecule. In
activated T cells, CD4 ligation may lead to a removal of essential
components of the TCR signaling complex and to a TCR desensitization
with reduced responses in terms of proliferation, cytokine production,
and AICD (see model in Fig. 8). Whether
the removal of SLP-76-associated proteins from the TCR/CD3 complex is
physical, e.g., due to a phosphorylation-independent translocation of
SLP-76/p56lck complexes to the cytoskeleton, is
presently under investigation.
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Acknowledgments |
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Footnotes |
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2 Address correspondence and reprint requests to Dr. Ottmar Janssen, Institute for Immunology, Christian-Albrechts-University, Brunswiker Strasse 4, D-24105 Kiel, Germany. E-mail address:
3 Abbreviations used in this paper: AICD, activation-induced cell death; FasL, Fas ligand; PLC, phospholipase C; PTK, protein tyrosine kinase; SEA, staphylococcal enterotoxin A superantigen; SH2 and SH3 domain, Src homology 2 and 3 domain, respectively; SLP-76, SH2 domain-containing leukocyte protein of 76 kDa; TCL, total cell lysate.
Received for publication February 24, 1999. Accepted for publication July 7, 1999.
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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  D. A. Rider, C. E.G. Havenith, R. de Ridder, J. Schuurman, C. Favre, J. C. Cooper, S. Walker, O. Baadsgaard, S. Marschner, J. G.J. vandeWinkel, et al. A Human CD4 Monoclonal Antibody for the Treatment of T-Cell Lymphoma Combines Inhibition of T-Cell Signaling by a Dual Mechanism with Potent Fc-Dependent Effector Activity Cancer Res., October 15, 2007; 67(20): 9945 - 9953. [Abstract] [Full Text] [PDF]
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 Y. Bogin, C. Ainey, D. Beach, and D. Yablonski SLP-76 mediates and maintains activation of the Tec family kinase ITK via the T cell antigen receptor-induced association between SLP-76 and ITK PNAS, April 17, 2007; 104(16): 6638 - 6643. |
) or
presence of anti-CD4 mAb OKT4. The cells were then pelleted, incubated
for 3 min in medium without (-) or with (+) SEA, and lysed in Brij96
lysis buffer. Immunoprecipitations of Vav and SLP-76 were performed
with 3 µg of the respective Ab and protein A- and protein G-Sepharose
beads (beads alone served as a control). Precipitated proteins were
separated by SDS-PAGE, and blotted to nitrocellulose. Blots were
developed with anti-pTyr mAb 4G10 (A) and reprobed
with anti-Vav (B) and anti-SLP-76
(C) Abs.
