The JI PBL Intereron Source
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Al-Daccak, R.
Right arrow Articles by Mourad, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Al-Daccak, R.
Right arrow Articles by Mourad, W.
The Journal of Immunology, 1998, 160: 225-232.
Copyright © 1998 by The American Association of Immunologists

Staphylococcal Enterotoxin D Is a Promiscuous Superantigen Offering Multiple Modes of Interactions With the MHC Class II Receptors1

Reem Al-Daccak2,*, Khalil Mehindate2,*, Farida Damdoumi*, Pierre Etongué-Mayer*, Helen Nilsson, Per Antonsson{dagger}, Michael Sundström{ddagger}, Mikael Dohlsten{dagger}, Rafick-Pierre Sékaly§ and Walid Mourad3,*

* Centre de Recherche en Rhumatologie Immunologie, Le Centre Hospitalier de l’Université Laval, Sainte-Foy, Quebec, Canada; {dagger} Pharmacia & Upjohn Research Center, Lund, Sweden; {ddagger} Pharmacia & Upjohn, Stockholm, Sweden; and § Laboratoire d’Immunology, Institut de Recherche Clinique de Montréal, Montreal, Quebec, Canada


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Dimerization of MHC class II molecules on the cell surface of human THP-1 monocytic cell line is a requirement for staphylococcal superantigen (SAG)-induced cytokine gene expression. The capacities of various SAG to induce this response are governed by their modes of interaction with MHC class II molecules. Staphylococcal enterotoxin A (SEA), with its two binding sites, dimerizes MHC class II molecules and subsequently induces cytokine gene expression in THP-1 cells. Here, we demonstrate that staphylococcal enterotoxin D (SED) and staphylococcal enterotoxin E (SEE) induce, similarly, IL-1ß and TNF-{alpha} gene expression in these cells. Using mutated toxins that lost their binding site with the MHC class II {alpha}- or ß-chain, we demonstrate that this response is also mediated by the dimerization of MHC class II molecules through two binding sites. Furthermore, SED forms Zn2+-dependent homodimers that allow multiple modes of MHC class II clustering, including ligation of {alpha}-chains ({alpha}/{alpha}), ß-chains (ß/ß), or the {alpha}- and ß-chains of two different class II molecules. The ß/ß interaction following Zn2+-dependent SED/SED homodimer formation seems to be mediated by the appearance of a novel binding site on SED that interacts with histidine 81 of the MHC class II ß-chain. The different modes of SED interactions also influence SED-induced T cell activation where simultaneous ligation of the {alpha}- and ß-chains is essential for optimal response. These various modes of SED binding may be used to preserve bivalency regardless of variability in the MHC class II {alpha}/ß/peptide complexes.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
Bacterial superantigens (SAGs)4 are a group of microbial products that have drastic effects on the immune system resulting from their high affinity interactions with the MHC class II molecules and the subsequent activation of T cells bearing particular TCR Vß element (1, 2, 3). Staphylococcus enterotoxins A, B, C1–3, D, and E (SEA to E) and the related toxin, the toxic shock syndrome (TSST-1) are among the most well-characterized SAGs. The structurally related toxins SEA, SED, and SEE share about 70 to 90% amino acid sequence homology, while these values decrease to 40 to 60% when compared with SEB, SECs, and TSST-1 (4). Despite this homology and the common binding to MHC class II molecules, staphylococcal SAGs differ in their affinities and their modes of interactions with their receptors.

Crystallographic and biochemical studies have revealed considerable information about the interaction of SAGs with the {alpha}- and ß-chains of class II molecules (5, 6, 7, 8, 9). SEB and TSST-1 both bind to the DR {alpha}-chain (8, 9); however, only TSST-1 extends over the peptide binding groove and partially interacts with bound peptides as well as the DR ß-chain (9). SEA bears two distinct, but cooperative, binding sites; the first is located in the C-terminal and contacts the DR ß-chain, whereas the second is located in the N-terminal and contacts the DR {alpha}-chain (10, 11). The binding of SEA to the ß-chain is Zn2+ dependent and displays high affinity for MHC class II molecules, whereas its binding to the {alpha}-chain is a low affinity and Zn2+ independent (11, 12). The crystal structure of SEA suggested strongly that one molecule of this toxin cannot interact with {alpha}- and ß-chains of the same class II molecule (13, 14), pointing out its binding to two different MHC class II molecules. Such binding may contribute to stabilize the TCR interaction that seems to be an absolute requirement for optimal SEA activity (15, 16). Indeed, further studies demonstrated that SAG-induced, T cell-independent cytokine gene expression in human monocytes and a monocytic cell line (THP-1) requires MHC class II dimerization (17, 18, 19). Thus, SEA is capable of inducing such a response (17, 18), whereas TSST-1 and SEB require cross-linking with bivalent specific Abs (18, 19). Engagement of CD40 on TSST-1- and SEB-stimulated monocytes or the addition of T lymphocytes can overcome this dimerization requirement (19, 20).

Based on the structural similarity of SEE, SED, and SEA, it is highly possible that SEE and SED share the same mode of interaction with class II molecules. The recent crystal structure indicated that SED has a unique property in forming Zn2+-dependent homodimers (SED/SED) in solution (21) that are coordinated by two Zn2+ ions. Each Zn2+ ion is coordinated by H218 from one SED molecule, and D182, H220, and D222 is coordinated from a second SED molecule. Whether this property influences the interactions of SED with class II molecules is not yet clear. In this study we address the above issues and demonstrate clearly that monomeric SED and SEE contain two MHC class II binding sites, have the capacity to dimerize class II molecules, and thus have a similar mode of interaction as SEA. Nevertheless, the SED/SED homodimer seems to be distinct from the other staphylococcal SAGs in its capacity to interact with two class II molecules in three different ways: 1) cross-linking of two {alpha}-chains ({alpha}/{alpha}), 2) cross-linking of two ß-chains (ß/ß), and 3) cross-linking of an {alpha}-chain and a ß-chain ({alpha}/ß). We also provide evidence that these different modes of interaction play a critical role in T cell activation.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Generation of recombinant SEA, SEE, and SED

SEA, SEE, and SED were cloned using PCR from crude DNA extract isolated from Staphyloccus aureus strains producing these enterotoxins. The translated part of the cDNA sequences of SEA and SEE were identical with published sequences (22, 23). The amino terminal of SEE, however, contains three additional amino acid residues (SEK). The SED cDNA sequence was identical with that previously reported (24), except for a single point mutation, C to G, substituting Pro84 to alanine. Direct sequence analysis of the PCR product revealed the same point mutation, indicating that this is a naturally occurring variant of SED. In vitro mutagenesis of SEA and SED was performed as previously described (10, 21). Three different SEA mutants were generated: 1) the aspartic acid at position 227 was substituted by alanine (SEAD227A); 2) the phenylalanine at position 47 was substituted by alanine (SEAF47A); and 3) double mutants in which F47 and D227 were substituted by alanines (SEAF47A/D227A). With respect to SED, we have generated four different mutants: 1) the phenylalanine at position 42 was substituted by alanine (SEDF42A); 2) the aspartic acid at position 182 was substituted by alanine (SEDD182A); 3) the histidine 218 was substituted by alanine (SEDH218A); and 4) the aspartic acid at position 222 was substituted by alanin (SEDD222A). The wild-type (wt) and mutated variants of SED are five amino acid residues shorter than SEA, similar to the published amino terminal sequence of SED (24), and are numbered accordingly. Escherichia coli expression and protein purification of wild-type and mutated variants of SEA, SEE, and SED were performed as previously described (10). The purity of these toxins was confirmed by SDS-PAGE followed by Coomassie blue or immunoblot with specific mAbs.

Cell lines

The THP-1 monocytic human cell line was obtained from American Type Culture Collection (Rockville, MD) and maintained in RPMI 1640 medium containing 10% FCS and antibiotics. This cell line expresses low levels of HLA-DR molecules, while it is completely negative for HLA-DQ and -DP (25). DAP-3 cells expressing HLA-DR1, HLA-DR1ß81A, or HLA-DR1{alpha}39A were previously described and maintained in MEM supplemented with 5% FCS and G418 (26). The murine CD4+ T cell hybridoma K25 that expresses Vß3 and responds to SED was maintained in RPMI supplemented with 10% FCS (27).

Northern blot analysis

Stimulation conditions for each experiment are detailed in the appropriate figure legends. RNA was purified using Trizol reagent (Life Technologies Products, Burlington, Canada) according to the manufacturer’s procedure, and 10 µg of RNA was loaded onto 1% agarose gels. The RNA was then transfered onto Hybond-N filter paper and was hybridized with random primer-labeled cDNA probes for IL-1ß and TNF-{alpha}. Equal loadings of RNA were confirmed by hybridization with the GAPDH cDNA probe. The mRNA hybridizing with the cDNA probes was visualized by autoradiography (28).

Western blot

Toxins were incubated with different concentrations of Zn2+ or EDTA in 0.1 HEPES buffer, pH 7.5, with 100 mM NaCl for 30 min at room temperature on a rotation platform. The cross-linking agent disuccinimidyl suberate (DSS; Pierce Chemcial Co., Rockford, IL) was added to a final concentration of 0.25 mM, and the mixtures were further incubated for 30 min. The reaction was stopped with SDS sample buffer, then samples were boiled and separated by electrophoresis on precast 12% Tris-glycine gels (Bio-Rad, Hercules, CA). The separated proteins were blotted onto nitrocellulose sheets, then incubated with a polyclonal rabbit anti-SED Abs (1 µg/ml). Blots were incubated with horseradish peroxidase-conjugated goat anti-rabbit (1/3000; Bio-Rad). ECL detection reagents and Hyperfilm ECL (Amersham, Life Science, Little Chalfont, U.K.) were used for detection.

Binding assays

The abilities of different toxins to bind HLA-DR molecules were assessed as previously described (26). Briefly, toxins were labeled with 125I, then 4 x 105 DAP-3 transfectants were incubated with 50 ng of 125I-labeled toxins in 200 µl of binding buffer (RPMI, 10 µM Zn2+, and 0.1% NaN3) for 3 h at 37°C. Cells were then pelleted through an oil cushion (84% silicon oil and 16% mineral oil), and their radioactivity in counts per minute was determined using a gamma counter.

T cell stimulation

DAP-3 transfectants were used as APCs to stimulate K25 T cell hybridoma with SEDwt or its mutants as previously described (26). Briefly, 2 x 104 APCs were incubated for 20 h in the presence of 8 x 104 T cells and various concentrations of toxins. Cocultures were performed in a final volume of 200 µl in 96-well flat-bottom plates at 37°C in 5% CO2. Stimulation was evaluated by the amount of IL-2 released by T cells in the coculture supernatants. Levels of IL-2 were determined by the ability of the coculture supernatant to support proliferation of the IL-2-dependent cell line CTLL2.


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
Activation of monocyte cytokine production by Zn2+-dependent staphylococcal enterotoxins is mediated by MHC class II {alpha}/ß-chain cross-linking

Certain MHC class II-mediated events can be induced by a monovalent ligand, while others, such as cytokine gene expression, require bivalent ligand that can trigger dimerization or even oligomerization of MHC class II molecules on the cell surface (17, 18, 29). Based on the structural homology among SEE, SED, and SEA, it is highly possible that SEE and SED act as bivalent cross-linkers of MHC class II molecules as described previously for SEA. This possibility was verified by analyzing the capacity of SEE and SED to induce IL-1ß and TNF-{alpha} in the THP-1 monocytic cell line. THP-1 cells were resuspended in FCS-free RPMI (Zn2+ free to ensure the absence of homodimeric form of SED) and stimulated with SED or SEE, and the induced responses were compared with those induced by different concentrations of SEA. Both toxins induced dose-dependent IL-1ß and TNF-{alpha} gene expression in THP-1 cells comparable to that induced by SEA (Fig. 1GoA). It is noteworthy that these toxins belong to the Zn2+-dependent staphylococcal superantigens, and the above experiment was conducted without supplementary ions; however, the Zn2+ that can be released from intracellular storage or the unstable Zn2+ on the MHC class II molecules seems to be sufficient to support their functional binding to their receptors (12). Accordingly, SEE- and SED-induced responses appear to be mediated by dimerization of class II molecules via their {alpha}/ß-chains.



View larger version (56K):
[in this window]
[in a new window]
  		FIGURE 1. Stimulation of the THP-1 monocytic cell line with SEA, SEE, and SED induces similar IL-1ß and TNF-{alpha} mRNA expression, which is completely inhibited by SEAF47A and SEAD227A pretreatment. A, Cells were washed twice with HBSS, resuspended in RPMI/serum-free medium (5 x 106 cells/ml), and stimulated with different concentrations (0.2, 1, or 5 µg/ml) of SEA, SEE, or SED for 1 h at 37°C. Cells were then harvested, RNA was extracted, and 10 µg of total RNA was loaded in each lane, electrophoresed, transferred to nitrocellulose filters, and hybridized with a probe for IL-1ß followed by TNF-{alpha} and the housekeeping gene GAPDH as a control for equal RNA loading. B, HBSS-washed cells were resuspended in RPMI/serum-free medium (5 x 106 cells/ml), pretreated for 30 min with medium alone or with 10 µg/ml of SEAF47A, SEAD227A, or SEAF47A/D227A, and then stimulated with 1 µg/ml of SEDwt or with SEEwt for 1 h at 37°C. The reaction was stopped, total RNA was purified, and the levels of IL-1ß and TNF-{alpha} mRNA were determined as described in A.

 
To confirm this mode of action, inhibition assays were performed using SEA mutants that can interact with a single MHC class II chain, either the {alpha}-chain (SEAD227A) or the ß-chain (SEAF47A). We have previously reported that stimulation of THP-1 with these mutants fails to induce IL-1ß and TNF-{alpha} expression, and their binding to the {alpha}- and ß-chains completely inhibits the SEAwt-induced response (18). Figure 1GoB shows that pretreatment of THP-1 cells with a 10-fold excess of SEAD227A or SEAF47A completely inhibited SED- and SEE-induced cytokine gene expression, whereas pretreatment with a double SEA mutant (SEAF47A/SEAD227A), which binds neither {alpha}- nor ß-chains, did not have any blocking effect. These data clearly indicate that blocking either class II {alpha}-chain or ß-chain leads to a complete loss of SED and SEE activities, supporting that both toxins cross-link an {alpha}-chain and a ß-chain of two class II molecules.

To further confirm this issue and to identify the residues involved in this response, we generated, by site-directed mutagenesis, SED mutants in which the N-terminal phenylalanine at position 42 (equivalent to SEAF47) was substituted by alanine (SEDF42A), or the C-terminal aspartic acid at position 222 (equivalent to SEAD227) was substituted by alanine (SEDD222A). Stimulation of THP-1 cells either with SEDF42A (expected to interact only with the ß-chain) or with SEDD222A (expected to interact only with the {alpha}-chain) in FCS- and Zn2+-free RPMI did not induce any detectable cytokine gene expression (Fig. 2GoA), confirming the implication of SEDF42 and SEDD222 in the interaction with class II molecules. Similar results were obtained with SEE mutated at the same residues (data not shown). When the SED mutants were used in inhibition assays, both SEDF42A and SEDD222A were able to abolish the capacity of SEAwt, SEEwt, and SEDwt to induce cytokine gene expression (Fig. 2GoB). Hence, the superantigenic activity, at least in inducing cytokine gene expression, of the three Zn2+-dependent staphylococcal superantigens is mediated by their interactions with the class II {alpha}-chain via F42 (or its equivalent depending on the SAG) and with the class II ß-chain via D222 (or its equivalent) that permit an {alpha}/ß cross-linking of these molecules.



View larger version (39K):
[in this window]
[in a new window]
  		FIGURE 2. Stimulation with SEDF42A and SEDD222A failed to induce IL-1ß and TNF-{alpha} mRNA expression in the THP-1 monocytic cell line, but completely inhibited SEAwt-, SEDwt-, and SEEwt-induced responses. A, Cells were washed twice with HBSS, resuspended in RPMI/serum-free medium (5 x 106 cells/ml), and stimulated with different concentrations (0.2, 1, and 5 µg/ml) of SEDwt, SEDF42A, or SEDD222A for 1 h at 37°C. B, HBSS-washed cells were resuspended in RPMI/serum-free medium (5 x 106 cells/ml), pretreated for 30 min with medium alone or with 10 µg/ml of SEDF42A or SEDD222A, and then stimulated with 1 µg/ml of SEAwt, SEDwt, or SEEwt for 1 h at 37°C. The reactions were stopped, and IL-1ß and TNF-{alpha} mRNA expressions were determined by Northern blot as described in Figure 1Go.

 
Interactions of SED/SED homodimer with MHC class II molecules

The recently elucidated SED crystal structure demonstrated the unique feature of SED (compared with SEA and SEE) in forming Zn2+-dependent homodimers (21). The SED dimerization, although reported with high Zn2+ concentrations, can be detected at concentrations as low as 1 µM. Since the residue D222 is implicated in SED functional binding to class II ß-chain and was described as essential for SED/SED dimer formation (21), it was interesting to analyze how these SED homodimers functionally interact with class II molecules. To this end, THP-1 cells were resuspended in FCS- and Zn2+-free RPMI, in RPMI supplemented with 1 µM Zn2+ (conditions that permit significant SED/SED dimer formation), or in RPMI supplemented with 5% FCS, then stimulated with SEDwt, SEDF42A, SEDD222A, SEDH218A, or SEDD182A. It is worth noting that substitution of any of the latter three residues by alanine completely abolishes SED/SED homodimer formation. Figure 3Go (A–C) demonstrates that under all the above experimental conditions, SEDwt, SEDH218A, and SEDD182A induce similar cytokine gene expression, suggesting that the activities of both SEDH218A and SEDD182A are mediated by {alpha}/ß class II cross-linking as monomeric SEDwt. On the other hand, SEDwt in the presence of Zn2+ forms its homodimers, and since the main SED functional residue of class II ß interactions is engaged in this dimer formation, it is highly likely that the SEDwt homodimers induce cytokine gene expression by cross-linking two {alpha}-chains of two different class II molecules. Unexpectedly, SEDD222A and SEDF42A induced significant IL-1ß and TNF-{alpha} gene expression in the presence of Zn2+ or 5% FCS (Fig. 3Go, B and C), whereas under the same conditions, SEAF47A, SEAD227A, SEEF47A, and SEED227A failed to induce any detectable response (data not shown). Since cross-linking of MHC class II molecules is a requirement for the SAG-induced response, these data indicate that both mutants are still interacting with two different MHC class II molecules.



View larger version (68K):
[in this window]
[in a new window]
  		FIGURE 3. Addition of Zn2+ leads to the formation of dimeric form of SEDF42A and SEDD222A and allows the induction of IL-1ß and TNF-{alpha} mRNA expression in the THP-1 monocytic cell line. Washed THP-1 cells were resuspended (5 x 106 cells/ml) in RPMI (A), RPMI containing 1 µM Zn2+ (B), or RPMI supplemented with 5% FCS (C) and then stimulated with two concentrations (0.5 or 2 µg/ml) of SEDwt, SEDF42A, SEDD222A, SEDH218A, or SEDD182A for 1 h at 37°C. Cells were harvested, RNA was extracted, and 10 µg of total RNA was loaded in each lane, electrophoresed, transferred to nitrocellulose filters, and hybridized with a probe for IL-1ß followed by TNF-{alpha} and the housekeeping gene GAPDH as a control for equal RNA loading.

 
To determine their mode of interaction along with that of SEDwt homodimers, we used inhibitory assays with the SEA mutants. THP-1 cells were preincubated with SEAF47A or SEAD227A for 30 min in the presence of 1 µM Zn2+, then were stimulated with SEDwt, SEDF42A, SEDD222A, SEDH218A, or SEDD182A. The SEDwt-induced response was abolished only by blocking both {alpha}- and ß-chains while blocking either chain was sufficient to abolish SEDH218A- and SEDD182A-induced responses (Fig. 4Go). Accordingly, it is clear that the cytokine-inducing activity of monomeric SED is mediated by {alpha}/ß cross-linking, whereas that of the homodimeric SED can be mediated by ß/ß and {alpha}/{alpha} interaction, confirming the three different modes of SED interaction previously suggested. SEDF42A-induced cytokine gene expression was inhibited only when the ß-chain was blocked, indicating clearly that this homodimeric mutant induces its response by ß/ß cross-linking. Since the D222 of this mutant is normally engaged in its dimer formation, it is highly possible that this mutant is interacting via a novel class II ß-chain binding site as a result of a Zn2+-induced SED/SED homodimer. The SEDD222A response was only abolished when THP-1 cells were treated with SEAD222A, which blocks the interaction with the {alpha}-chain; thus, it is probably interacting with two class II {alpha}-chains. However, for this to take place, SEDD222A has to be able to form homodimers. This possibility was investigated in a covalent cross-linking test where toxins (350 nM) were preincubated with 100 µM EDTA, 1 µM Zn2+ (under the same conditions as those used in the above experiments), or 100 µM for 30 min. The Western blot results on DSS cross-linked toxins (Fig. 5Go) demonstrate clearly that the presence of 1 µM Zn2+ is sufficient to induce significant dimerization of SEDwt. However, in the presence of neither high (100 µM) nor low (1 µM) concentrations of Zn2+ were we able to detect any dimer formation with SED mutants. Although the detection of SEDD222A dimers was not biochemically possible, it seems that this mutant forms dimers that can be functionally detectable. Possibly, the formation of functional dimers might be facilitated by prior binding of SED monomers to MHC class II molecules {alpha}-chains on the cell surface.



View larger version (32K):
[in this window]
[in a new window]
  		FIGURE 4. Modulation of SEDwt-, SEDF42A-, SEDD222A-, SEDH218A-, or SEDD182A-induced IL-1ß and TNF-{alpha} mRNA expression in the THP-1 cell line by pretreatment with SEAF47A and SEAD227A. Washed THP-1 cells were resuspended (5 x 106 cells/ml) in RPMI containing 1 µM Zn2+, pretreated with medium alone or with 10 µg/ml of SEAF47A, SEAD227A, or SEAF47A plus SEAD227A for 30 min, and then stimulated with 1 µg/ml of SEDwt (A), SEDF42A (B), SEDD222A (C), SEDD182A (D), or SEDH218A (E) for 1 h at 37°C. Cells were harvested, RNA was extracted, and 10 µg of total RNA was loaded in each lane, electrophoresed, transferred to nitrocellulose filters, and hybridized with a probe for IL-1ß followed by TNF-{alpha} and the housekeeping gene GAPDH as a control for equal RNA loading.

 


View larger version (55K):
[in this window]
[in a new window]
  		FIGURE 5. Western blot analysis of Zn+-induced SED dimerization. SEDs, in a concentration of 350 nM, were incubated with 100 µM EDTA (lane 1), 1 µM Zn2+ (lane 2), or 100 µM Zn2+ (lane 3) for 30 min. The cross-linking agent DSS was added, and toxins were incubated for an additional 30 min. Samples were separated by electrophoresis, transfered, and blotted with polyclonal rabbit anti-SED Ab.

 
SED/SED dimer interacts with histidine 81 on the ß-chain of HLA-DR1 via a novel binding site

Since D222 of SED is normally engaged in its dimer formation, we suggested above that Zn2+-induced SED/SED homodimer formation can lead to the appearance of a novel binding site on SED. Indeed, the ability of SEAF47A (which interacts with the ß-chain via H81) to inhibit the SEDF42A-induced response suggests that this novel SED binding site interacts with histidine 81 of the class II HLA-DR ß-chain. Hence, binding experiments were conducted in the presence of 10 µM Zn2+ (to permit maximal SED homodimer formation) using radiolabeled SEDwt, presenting both dimeric and monomeric forms, SEDH218A, presenting the monomeric form, SEA as a control, and DAP cells transfected with HLA-DR1 or HLA-DR1 ß81A. Figure 6Go demonstrates that both SED dimers and monomers bind significantly to DAP HLA-DR1-transfected cells. Substitution of histidine 81 by alanine completely abolished SED binding to class II, indicating that the SED/SED dimer that bears the novel binding site and the SED monomer bind to the ß-chain of HLA-DR through histidine 81. Similar results were obtained with SEA, while TSST-1 binding, reported to be mainly via the HLA-DR {alpha}-chain, was not affected by the presence or the absence of histidine 81.



View larger version (20K):
[in this window]
[in a new window]
  		FIGURE 6. Histidine 81 on the ß-chain plays a critical role in SED/SED homodimer binding. DAP-DR1 or DAP-DR1ßH81 cells (5 x 105 in RPMI supplemented with 10 µM Zn2+) were incubated with 20 ng of 125I-labeled toxins for 3 h, then toxins bound to the cells were separated from free ones and counted with a gamma counter.

 
Simultaneous ligation of class II {alpha}- and ß-chains is required for optimal activation of T cell clones by SED

The data provided above indicate that SED interacts with MHC class II molecules in different forms. In the following experiments we investigated the significance of these different modes of interaction on the activation of T lymphocytes. To this end we used a functional assay, IL-2 production, and the murine T cell hybridoma K25 that is known to respond to SED via its Vß3. Our results (Fig. 7Go) clearly indicate that SED can trigger the activation of this T cell hybridoma only when presented via {alpha}- and ß-chains of two different MHC class II molecules. Presenting SED by either {alpha}/{alpha} or ß/ß of MHC class II molecules failed to induce any significant IL-2 production by this hybridoma. Complementary experiments using DAP-3 cells transfected with HLA-DR1ß81A or HLA-DR1{alpha}39A showed the failure of the K25 clone to produce detectable IL-2 when stimulated with SEDwt, supporting the idea that interaction of SED with both chains of class II molecules is required for efficient T cell activation.



View larger version (18K):
[in this window]
[in a new window]
  		FIGURE 7. Simultaneous binding of SED to the {alpha}- and ß-chains of two different class II molecules is required for K25 T cell hybridoma activation. Presentation of SEDwt, SEDF42A, SEDD222A, and SEDH218A to the T cell hybridoma K25 (Vß3) by DAP cells expressing wild-type HLA-DR1 is shown. The stimulation response is expressed as units of IL-2.

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
The primary role of MHC class II molecules as receptors able to present the naturally occurring microbial products SAGs to T cells (1, 2, 3) has been extended to include the capacity of these molecules to act as signal transducers through which activation of class II-positive cells emerges (28). The panel of cytokines and cell activation accompanying the SAG/class II interaction proposed the implication of these events in the pathology of SAG-producing bacteria (30, 31, 32, 33, 34). Although the precise interaction sites between SAGs and class II have not been fully defined, structural and crystallographic studies have provided considerable information, at least for staphylococcal SAGs (8, 9, 10, 11, 12, 14, 26, 35, 36, 37, 38).

Staphylococcal SAGs can be divided into two major subfamilies based on their interactions with class II: a zinc-coordinated subfamily, including SEA, SEE, and SED, and a zinc-independent subfamily, including SEB and TSST-1. Given the high degree of similarity between the members of the zinc-coordinated subfamily, it was expected that these toxins bind class II with similar orientations. Our previous functional analysis of the SEA/class II interaction confirmed these data and strongly favored the idea that SEA forms a class II2SEA1 trimer configuration that permits dimerization of class II molecules and cell activation (18). In this report we provide functional data supporting the idea that SEE and SED also interact with class II via two distinct binding sites that probably allow dimerization of MHC class II molecules and subsequent cytokine gene expression. Indeed, the capacity of staphylococcal SAGs to induce cytokine gene expression in human monocytes seems to be governed by their capacity to dimerize or oligomerize these receptors.

Results obtained during recent years have given ample evidence that several cell surface receptors, including growth factors and cytokine receptors, are activated by ligand-induced dimerization or oligomerization and that this mechanism is of general applicability for the regulation of signal transduction (39). Cell activation can be obtained either by heterodimerization, as for Ile receptors, or by homodimerization, as for growth hormone receptors (40, 41, 42). SAG-induced activation of MHC class II-positive cells seems to be mostly mediated by a Zn2+-dependent receptor homodimerization. Interestingly, binding of human growth hormone to human prolactin receptor is also zinc coordinated, and tight binding of zinc occurs only in the presence of receptor and ligand (43). Hence, under normal physiologic conditions it is highly likely that SEA, SEE, and SED interact with class II and activate class II-positive cells by a mode of interaction similar to that reported hereby.

The crystal structure of SED revealed a unique feature of SED to form zinc-dependent SED/SED homodimers, which in the interface coordinates two Zn2+ by participation of amino acid residues D182, H220, and D222 from one molecule and H218 from the other (21). Our results implicates that this particular feature of SED allows its interaction with MHC class II molecules in three different ways: cross-linking of HLA-DR{alpha}/SED-SED/HLA-DR{alpha} or HLA-DRß/SED-SED/HLA-DRß forming tetrameric complexes or cross-linking of HLA-DR{alpha}/SED/HLA-DRß forming trimeric complexes. The SED residues implicated in Zn2+ coordination are at a homologous position as those previously described in SEA (13). In the SEA molecule, zinc coordination by these residues controls the formation of stable SEA/class II complexes. However, in SED these residues control both SED/SED homodimer formation and the interaction with MHC class II molecules. The relative contributions of individual SEA residues to Zn2+ coordination are in the order D227>H225>H187 (11). Our results indicate that the presence of D222 in SED is crucial for monomeric interaction with MHC class II, but is less important for the formation of SED/SED homodimer. In contrast, SEDD182 and SEDH218 have a minor or no role in regulating the Zn2+-dependent interaction with class II, but they are critical for the formation of SED/SED homodimer. The most surprising observation was the ability of the SED/SED homodimer to interact with two ß-chains of different class II molecules. It appears, therefore, that Zn2+-induced SED/SED homodimer formation can also lead to the appearance of a novel site on SED/SED that can interact with histidine 81 on the class II ß-chain. Although the SED residue D222 is more important for the interaction with the ß-chain than for SED/SED dimer formation, it is still critical for the exposure of the novel dimer binding site, at least under our experimental conditions (1 µM Zn2+). Alternatively, in our recent crystal studies we detected a second bound Zn2+ ion in SED located at the interdomain interface (21). Microcalorimetry demonstrated that binding of Zn2+ to this site required severalfold higher Zn2+ concentrations than that to the classical Zn2+ domain. Thus at high Zn2+ concentrations, it is possible that the second Zn2+ site can be occupied and consequently mediate binding with the class II ß-chain. Although the data presented in this paper do not support the interaction of monomeric SED with the ß-chain of MHC class II molecules via the novel binding site, one cannot rule out completely that addition of a high concentration of Zn2+ (>1 µM) may lead to the appearance of this novel binding site on monomeric SED. Studies are in progress to clarify these possibilities and to define and characterize the novel SED binding site. Together, these studies support the idea that SED has dynamic ways to interact with MHC class II molecules and can be considered, at present, among the most promiscuous known SAG.

What is the significance of the variety of multimeric binding modes between SAGs and class II receptors? Our present and previously reported results (18, 19) clearly demonstrate that the modes of interaction of staphylococcal SAGs with the class II molecules control their ability to activate MHC class II-positive cells. The major role of MHC class II molecules in SAG biology has been attributed to their role in the presentation of SAG to T lymphocytes (1). Presentation of SAGs to TCR can also be achieved by immobilization of SAG on solid phases (44), but this response has a much lower amplitude than that observed when SAGs are presented by MHC class II-positive cells. Indeed, presentation of SAG in the absence of class II activates only a fraction of TCR Vß families, whereas other TCR Vß families require MHC class II presentation (15). This effect may reflect a role for class II in stabilizing low affinity interactions with certain TCR Vß-chains. With respect to SEA, recent evidence indicates that binding of this toxin to the {alpha}- and ß-chains of class II molecules plays a critical role in positioning SEA for appropriate interaction with certain TCR Vß elements (16). Although SED can interact with class II molecules using three different modes ({alpha}/ß, {alpha}/{alpha}, and ß/ß), only SED bound to {alpha}- and ß-chains of two different class II molecules can induce significant activation of T cell hybridoma expressing the Vß3 element. Regardless of whether the same mode of interaction is required for SED recognition by other Vß elements is not yet clear, our data indicate that different modes of SED interaction can have a major role in T cell activation and point out the importance of toxin positioning in this response. The multiple binding modes between SAG and MHC class II will permit the formation of unique configurations that may allow the SAG to be recognized by certain TCR. Accordingly, it is possible that the T cell repertoire that is expanded by a given SAG is determined by distinct SAG/class II interactions. The induction of stimulatory cytokines, e.g., IL-1, during SAG/class II interactions may further contribute to amplify T cell responses. In the SAG system, monocyte cytokine production is induced following class II ligation, which is controlled, at least in part, by the topology of the SAG/class II interaction. In this context the interaction of SAG with class II will influence directly and indirectly the T cell and inflammatory responses.

Together, these data may contribute to clarify why a micro-organism such as S. aureus produces a variety of toxins that share the ability to induce certain effects in the host. The multiple binding modes between SAG and MHC class II may facilitate the survival of the micro-organism in a broad range of molecular environments by bypassing variability in the MHC/peptide complex and thus ensure its existence in various hosts and cell types. Increased understanding of how these toxins interact with cells of the immune system will permit an understanding of or further unravel their pathologic effects.


   Acknowledgments
 
The authors thank Dr. Kappler for providing the T cell hybridoma.


   Footnotes
 
1 This work was supported by grants to W.M. from the Medical Research Council and the Arthritis Society, scholarships from le Fonds de la Recherche en Santé du Québec (to W.M. and R.A.-D.), and a Medical Research Council scientist award (to R.P.S.). Back

2 The first two authors contributed equally to this work. Back

3 Address correspondence and reprint requests to Dr. Walid Mourad, Centre de recherche en Rhumatologie-Immunologie, Centre Hôspitalier de l’université Laval, Room T1–49, 2705 blvd. Laurier, Ste-Foy, Quebec, Canada G1V-4G2. Back

4 Abbreviations used in this paper: SAG, superantigen; SEA, staphylococcal enterotoxin A; SEB, staphylococcal enterotoxin B; SED, staphylococcal enterotoxin D; SEE, staphylococcal enterotoxin E; TSST-1, toxic shock syndrome toxin-1; wt, wild type; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DSS, disuccinimidyl suberate. Back

Received for publication July 9, 1997. Accepted for publication September 18, 1997.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

  1. Scherer, M. T., L. Igonatowicz, G. M. Winslow, J. W. Kappler, P. Marrack. 1993. Superantigens: bacterial and viral proteins that manipulate the immune system. Annu. Rev. Cell. Biol. 9:101.
  2. Scholl, P., A. Diez, W. Mourad, J. Parsonnet, R. S. Geha, T. Chatilla. 1989. Toxic shock syndrome toxin 1 binds to major histocompatibility complex class II molecules. Proc. Natl. Acad. Sci. USA 86:4210.[Abstract/Free Full Text]
  3. Fraser, J. D.. 1989. High-affinity binding of staphylococcal enterotoxin A and B to HLA-DR. Nature 339:221.[Medline]
  4. Thibodeau, J., R. P. Sékaly. 1995. Bacterial Superantigens: Structure, Function and Therapeutical Potential Springer, R. G. Landes Co., Georgetown.
  5. Dellabona, P., J. Peccoud, J. Kappler, C. Benoist, D. Mathis. 1990. Superantigens interact with MHC class II molecules outside of the antigen groove. Cell 62:1115.[Medline]
  6. Chintagumpala, M. M., J. A. Mollick, R. R. Rich. 1991. Staphylococcal toxins bind to different sites on HLA-DR. J. Immunol. 147:3876.[Abstract]
  7. Scholl, P. R., A. Deiz, R. S. Geha. 1989. Staphylococcal enterotoxin B and toxic shock syndrome toxin-1 bind to distinct sites on HLA-DR and HLA-DQ molecules. J. Immunol. 143:2583.[Abstract]
  8. Jardetzky, T. S., J. H. Brown, J. C. Gorga, L. J. Stern, R. G. Urban, Y.-I. Chi, C. Stauffacher, J. L. Strominger, D. C. Wiley. 1994. Three-dimensional structure of human class II histocompatibility molecule complexed with superantigen. Nature 368:711.[Medline]
  9. Kim, J., R. G. Urban, J. L. Strominger, D. C. Wiley. 1994. Crystallographic structure of toxic syndrome toxin-1 complexed with a human class II major histocompatibility molecule HLA-DR1. Science 266:1870.[Abstract/Free Full Text]
  10. Abrahmsen, L., M. Dohlsten, S. Segren, P. Bjork, E. Jonsson, T. Kalland. 1995. Characterization of two distinct MHC class II binding sites in the superantigen staphylococcal enterotoxin A. EMBO J. 14:2978.[Medline]
  11. Hudson, K. R., R. E. Tiedemann, R. G. Urban, S. C. Lowe, J. L. Strominger, J. D. Fraser. 1995. Staphylococcal enterotoxin A has two cooperative binding sites on major histocompatibility complex class II. J. Exp. Med. 182:711.[Abstract/Free Full Text]
  12. Fraser, J. D., R. G. Urban, J. L. Strominger, H. Robinson. 1992. Zinc regulates the function of two superantigens. Proc. Natl. Acad. Sci. USA 89:5507.[Abstract/Free Full Text]
  13. Schad, E. M., I. Zaitseva, V. N. Zaitsev, M. Dohlsten, T. Kalland, P. M. Schievert, D. H. Ohlendorf, L. A. Svensson. 1995. Crystal structure of the superantigen staphylococcal enterotoxin type A. EMBO J. 14:3292.[Medline]
  14. Ulrich, R. B., S. Bavari, M. A. Olson. 1995. Staphylococcal enterotoxins A and B share a common structural motif for binding class II major histocompatibility complex molecules. Nat. Struct. Biol. 2:554.[Medline]
  15. Newton, D. W., M. Dohlsten, C. Olsson, S. Segrén, K. E. A. Lundin, P. A. Lando, T. Kalland, M. Kotb. 1996. Mutations in the MHC class II binding domains of staphylococcal enterotoxin A differentially affect T cell receptor Vß specificity. J. Immunol. 157:3988.[Abstract]
  16. Thibodeau, J., M. Dohlesten, I. Cloutier, P. M. Lavoie, P. Bjork, F. Michel, C. Léveillé, W. Mourad, T. Kalland, R.-P. Sékaly. 1997. Molecular characterization and role in T cell activation of staphylococcal enterotoxin A binding to HLA-DR {alpha}-chain. J. Immunol. 158:3698.[Abstract]
  17. Tiedemann, R. E., J. D. Fraser. 1996. Cross-linking of MHC class II molecules by staphylococcal enterotoxin A is essential for antigen-presenting cell and T cell activation. J. Immunol. 157:3958.[Abstract]
  18. Mehindate, K., J. Thibodeau, M. Dohlsten, T. Kallad, R. Sekaly, W. Mourad. 1995. Cross-linking of major histocompatibility complex class II molecules by staphylococcal enterotoxin A superantigen is a requirement for inflammatory cytokine gene expression. J. Exp. Med. 182:1573.[Abstract/Free Full Text]
  19. Mehindate, K., R. Al-Daccak, F. Damdoumi, W. Mourad. 1996. Synergistic effect between CD40 and class II signals overcomes class II dimerization requirement in superantigen-induced cytokine gene expression. Eur. J. Immunol. 26:2075.[Medline]
  20. See, R. H., W. W. S. Kum, A. H. Chang, A. W. Chow. 1992. Induction of TNF and IL-1 by purified staphylococcal toxic shock syndrome toxin 1 requires the presence of both monocytes and T lymphocytes. Infect. Immun. 60:4957.[Abstract/Free Full Text]
  21. Sundström, M., L. Abrahmsén, P. Antonsson, M. Dohlsten. 1996. The crystal structure of staphylococcal enterotoxin type D reveals Zn2+ mediated homodimerisation. EMBO J. 15:6832.[Medline]
  22. Betley, M. J., J. J. Mekalanos. 1988. Nucleotide sequence of the type A staphylococcal enterotoxin gene. J. Bacteriol. 170:34.[Abstract/Free Full Text]
  23. Couch, J. L., M. T. Soltis, M. J. Betley. 1988. Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene. J. Bacteriol. 170:2954.[Abstract/Free Full Text]
  24. Bayles, K. W., J. J. Iandolo. 1989. Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D. J. Bacteriol. 171:4799.[Abstract/Free Full Text]
  25. Yunis, J. J., H. Band, F. Bonneville, E. J. Yunis. 1989. Differential expression of MHC class II antigens in myelomonocytic leukemia cell lines. Blood 73:931.[Abstract/Free Full Text]
  26. Thibodeau, J., I. Cloutier, P. M. Lavoie, N. Labrecque, W. Mourad, T. Jardetzky, R. P. Sékaly. 1994. Subsets of HLA-DR1 molecules defined by SEB and TSST-1 binding. Science 266:1874.[Abstract/Free Full Text]
  27. Herman, A., G. Croteau, R.-P. Sékaly, J. Kappler, P. Marrack. 1990. HLA-DR alleles differ in their ability to present staphylococcal enterotoxins to T cells. J. Exp. Med. 127:709.
  28. Sambrook, J., E. F. Fritsch, T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  29. Aoudjit, F., R. Al-Daccak, C. Léveille, W. Mourad. 1995. Signaling via MHC class II molecules: its effects on the immune system and its possible mechanisms. J. Thibodeau, and R. P. Sékaly, eds. Bacterial Superantigens: Structure, Function and Therapeutical Potential 147. Springer, R. G. Landes Co., Georgetown.
  30. Mourad, W., K. Mehindate, T. Schall, S. McColl. 1992. Engagement of Major histocompatibility complex class II molecules by superantigens induces inflammatory cytokine gene expression in human rheumatoid fibroblast-like synoviocytes. J. Exp. Med. 175:613.[Abstract/Free Full Text]
  31. Mehindate, K., R. Al-Daccak, J. Dayer, B. P. Kennedy, C. Kris, P. E. Poubelle, W. Mourad. 1995. Signalling via MHC class II induces PGE2-dependent interstitial collagenase gene expression in human fibroblast-like synoviocytes. J. Immunol. 22:3570.
  32. Al-Daccak, R., B. Damaj, P. H. Naccache, W. Mourad. 1995. Superantigens initiate CD4+ T cell/B cell interactions leading to early activation and proliferation of B cells. Eur. J. Immunol. 25:2539.[Medline]
  33. Kotzin, B. L., D. Y. Leung, J. Kappler, P. Marrack. 1993. Superantigens and their potential role in human disease. Adv. Immunol. 19:207.
  34. Uchiyama, T., X.-J. Yan, K. Imanishi, J. Yagi. 1994. Mechanism of T cell activation by the superantigens and their role in the pathogenesis of infectious diseases. Microbiol. Immunol. 38:245.[Medline]
  35. Tiedemann, R. E., R. J. Urban, J. L. Strominger, J. D. Fraser. 1995. Isolation of HLA-DR1.(staphylococcal enterotoxin A)2 trimers in solution. Proc. Natl. Acad. Sci. USA 92:12156.[Abstract/Free Full Text]
  36. Karp, D. R., E. O. Long. 1992. Identification of HLA DRß chain residues critical for binding staphylococcal enterotoxin A and E. J. Exp. Med. 175:415.[Abstract/Free Full Text]
  37. Bonin, A. V., S. Ehrlich, G. Malcherek, B. Fleischer. 1995. Major histocompatibility complex II-associated peptides determine the binding of the superantigen toxic shock syndrome toxin-1. Eur. J. Immunol. 25:2894.[Medline]
  38. Kozono, H., D. Parker, J. White, P. Marrack, J. Kappler. 1995. Multiple binding sites for bacterial superantigens on soluble class II MHC molecules. Immunity 3:187.[Medline]
  39. Heldin, C. H.. 1995. Dimerization of cell surface receptors in signal transduction. Cell 80:213.[Medline]
  40. Cunningham, B. C., M. Ultsch, A. M. de Vos, M. G. Mulkerrin, K. R. Clausner, J. A. Wells. 1991. Dimerization of the extracellular domain of the human growth hormone receptor by a single hormone molecule. Science 254:821.[Abstract/Free Full Text]
  41. de Vos, A. M., M. Ultsch, A. A. Kossiakoff. 1992. Human growth hormone and extracellular domain of its receptor: crystal structure of the complex. Science 255:306.[Abstract/Free Full Text]
  42. Ultsch, M., A. M. de Vos, A. A. Kossiakoff. 1991. Crystals of the complex between human growth hormone and the extracellular domain of its receptor. J. Mol. Biol. 22:865.
  43. Cunnigham, B. C., S. Bass, G. Fuh, J. A. Wells. 1990. Zinc mediation of the binding of human growth hormone of the human prolactin receptor. Science 250:1709.[Abstract/Free Full Text]
  44. Hamad, A. R. A., A. Herman, P. Marrack, J. Kappler. 1994. Monoclonal antibodies defining functional sites on the toxin superantigen staphylococcal enterotoxin B. J. Exp. Med. 180:615.[Abstract/Free Full Text]



This article has been cited by other articles:


Home page
 J. Immunol.Home page
N. Pumphrey, A. Vuidepot, B. Jakobsen, G. Forsberg, B. Walse, and K. Lindkvist-Petersson
Cutting Edge: Evidence of Direct TCR {alpha}-Chain Interaction with Superantigen
J. Immunol., September 1, 2007; 179(5): 2700 - 2704.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
D. D. Pless, G. Ruthel, E. K. Reinke, R. G. Ulrich, and S. Bavari
Persistence of Zinc-Binding Bacterial Superantigens at the Surface of Antigen-Presenting Cells Contributes to the Extreme Potency of These Superantigens as T-Cell Activators
Infect. Immun., September 1, 2005; 73(9): 5358 - 5366.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. V. Geisbrecht, B. Y. Hamaoka, B. Perman, A. Zemla, and D. J. Leahy
The Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens
J. Biol. Chem., April 29, 2005; 280(17): 17243 - 17250.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. C. Papageorgiou, M. D. Baker, J. D. McLeod, S. K. Goda, C. N. Manzotti, D. M. Sansom, H. S. Tranter, and K. R. Acharya
Identification of a Secondary Zinc-binding Site in Staphylococcal Enterotoxin C2: IMPLICATIONS FOR SUPERANTIGEN RECOGNITION
J. Biol. Chem., January 9, 2004; 279(2): 1297 - 1303.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-A. Langlois, Y. E. Fakhry, and W. Mourad
Zinc-binding Sites in the N Terminus of Mycoplasma arthritidis-derived Mitogen Permit the Dimer Formation Required for High Affinity Binding to HLA-DR and for T Cell Activation
J. Biol. Chem., June 13, 2003; 278(25): 22309 - 22315.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Petersson, H. Pettersson, N. J. Skartved, B. Walse, and G. Forsberg
Staphylococcal Enterotoxin H Induces V{alpha}-Specific Expansion of T Cells
J. Immunol., April 15, 2003; 170(8): 4148 - 4154.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
J.-G. Castaigne, W. Guo, C. Leveille, D. Charron, and R. Al-Daccak
A CD18-dependent protein kinase C {beta}-mediated alternative cell death pathway of activated monocytes
Int. Immunol., September 1, 2002; 14(9): 1003 - 1014.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. V. Golovkina, Y. Agafonova, D. Kazansky, and A. Chervonsky
Diverse Repertoire of the MHC Class II-Peptide Complexes Is Required for Presentation of Viral Superantigens
J. Immunol., February 15, 2001; 166(4): 2244 - 2250.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Nilsson, P. Bjork, M. Dohlsten, and P. Antonsson
Staphylococcal Enterotoxin H Displays Unique MHC Class II-Binding Properties
J. Immunol., December 15, 1999; 163(12): 6686 - 6693.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Al-Daccak, R.
Right arrow Articles by Mourad, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation