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Studies on Nucleoprotein Determinants for Systemic Lupus Erythematosus Serum¹

From the Department of Pharmacology, Tufts University School of Medicine, Boston, Massachusetts

Abstract

Serum of a patient with systemic lupus erythematosus (SLE) was examined in quantitative C' fixation with deoxyribonucleic acid (DNA), nucleoprotein and a variety of histone-DNA complexes. At least two distinct determinants were found for this serum. Some of the antibodies reacted with a structure which was best formed by histone and native DNA, in a weight ratio of 3:1 or higher. Inhibition and absorption experiments indicated that native DNA itself contributed a major part of the antigenic determinant. Moderate aggregation may have been important in forming this structure, but extensive aggregation reduced its reactivity. Of the histone fractions, the lysine rich F1(A) was most effective in forming the antigen. A second type of determinant, which was best formed by the arginine rich F2a and denatured DNA, was not inhibited by free DNA, but was inhibited by excess of the free histone fraction.

Footnotes

¹ This work was performed under Grant GB-5606 from the National Science Foundation.

² Present address: Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts.