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Hemolytic Plaque Formation by Mouse Spleen Cells Producing Antibodies against Rabbit Immunoglobulin G¹

From the Department of Pathology and Hygiene, College of Veterinary Medicine, University of Illinois, Urbana, Illinois

Abstract

Sheep erythrocytes sensitized with the immunoglobulin G (IgG) fraction of commercial rabbit anti-sheep hemolysin were not hemolyzed directly by complement, but were hemolyzed after treatment with anti-rabbit globulin and complement. Erythrocytes so sensitized were plated in nonanticomplementary agar, which also contained spleen cells from mice immunized with rabbit IgG. After incubation and addition of complement, hemolytic plaques were found around single spleen cells producing antibodies specific for rabbit IgG. The primary and secondary responses of mice to both aggregate-free and heat-aggregated rabbit IgG are described.

Footnotes

These studies were supported by Contract Nonr-1834(37) (NR 106–509) with the Office of Naval Research, Department of the Navy, and by Contract COO-1628-3 with the United States Atomic Energy Commission.