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The Journal of Immunology, 1967, 99: 849-858.
Copyright © 1967 by The American Association of Immunologists, Inc.
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Antigenic Structure of {gamma}E-Gobulin and Reaginic Antibody1

Kimishige Ishizaka, Teruko Ishizaka and William D. Terry

From the Children's Asthma Research Institute and Hospital, Denver, Colorado, and The Immunology Branch, National Cancer Institute, The National Institutes of Health, Bethesda, Maryland

Abstract

Both {kappa} and {lambda}-chain antigenic determinants were detected in {gamma}E-globulin by radioimmunoelectrophoresis. In addition, {gamma}E-globulin has antigenic determinants which are lacking in other immunoglobulins of the four known classes, i.e., {gamma}G, {gamma}A, {gamma}M and {gamma}D as well as in {gamma}A- and {gamma}G-subclasses. The protein did not contain the major antigenic determinants of the four known classes of immunoglobulins. The results indicate that {gamma}E-globulin represents a distinct class of immunoglobulin.

Reaginic activity in atopic patients' sera was precipitated by anti-{gamma}E-antibody but not by either {gamma}M-anti-{gamma}M-antibody complexes or {gamma}G-antibody complexes containing {gamma}G2-, {gamma}G3- and {gamma}G4-globulins. The distribution of reaginic activity in chromatographic fractions did not parallel that of {gamma}G2- or {gamma}G3-globulin. Passive sensitization of human skin with reaginic antibody was not blocked by any of the myeloma proteins of known classes or subclasses, whereas a fraction containing {gamma}E-globulin had blocking activity. The results indicate that reaginic antibody activity is associated with {gamma}E-globulin.

Footnotes

This work was supported mostly by Research Grant AI-04985 from the United States Public Health Service and partly by Research Grant GB-4646 from the National Science Foundation. The paper was presented before the 51st annual meeting of the American Association of Immunologists, Chicago, Ill., April 16 to 21, 1967.






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