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Sites of Synthesis of Serum Proteins

I. Serum Proteins Produced by Macrophages in Vitro¹

From the Department of Pathology, New York University School of Medicine, New York, New York

Abstract

Various cell types from adult rats, including thoracic duct cells, peripheral blood leukocytes, fractions of peritoneal exudate cells rich in mast cells, eosinophils or macrophages (monocytes), were examined for their ability to incorporate C¹⁴-amino acid into serum proteins. Autoradiography of immunoelectrophoretic patterns prepared with concentrated culture fluids demonstrated that macrophages, isolated by means of their adherence to glass, were much more active than any other cell type in the production of _1C and transferrin.

Peritoneal and lung macrophages from various other species, including mice, rabbits and guinea pigs, invariably produced _1C, and in the case of primates also _1E. The role of macrophages in the production by various tissues of serum proteins associated with the complement system was discussed.

Rats and mice were the only species studied in which transferrin was produced by lymphoid tissue and peritoneal macrophages.

Footnotes

These studies were supported by Grant AI-03076 from the United States Public Health Service. This work was submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (V.J.S.), New York University, 1966.

² Fellow of the United States Public Health Service, National Institute of General Medical Sciences (5-F1-GM-20043).

³ Recipient of Research Career Development Award GM-K3-15,522 from the United States Public Health Service.